The purpose of this study is to examine the effects of gait training using functional electrical stimulation on the improvement of hemiplegic patients' functions for balance and gait velocity. The subjects of the experiment were determined to be 10 each hemiplegic patients who had been diagnosed with stroke or brain damage six months or longer earlier assigned to an experimental group and a control group respectively. The subjects were evaluated before the experiment using Tetrax and 10M gait tests, received gait training five times a week for four weeks using functional electrical stimulation and were evaluated after the experiment in the same method as used in the evaluation before the experiment. In order to examine differences between the experimental group that received gait training using functional electrical stimulation and the control group that was treated by functional electrical stimulation and received gait training thereafter, differences between before and after the experiment were analyzed using paired sample t-tests and differences in changes after the experiment between the experimental group and the control group were analyzed using independent sample t-tests in order to compare the two groups with each other. Experimental results showed significant differences in weight bearing, balance and gait velocity between before and after the experiment in the experimental group(p<.05). In the control group, whereas weight bearing and gait velocity did not show any significant difference between before and after the experiment(p>.05), balance showed significant differences(p<.05). Weight bearing, balance and gait velocity change rates showed significant differences between the experimental group and the control group(p<.05). In conclusion, it was indicated that gait training using functional electrical stimulation is effective for enhancing stroke patients' weight bearing rates, balance abilities and gait velocity.

Immuno-MemBlot is a technique for detecting, analyzing, and identifying proteins, similar to the Western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular or slot templates directly onto the membrane. Recently we developed a new Immuno-MemBlot (IMB) method applying immunoreactions and coloring procedures directly in the wells of MemBlot apparatus, which were connected by canals to perform drainage for reagent application and buffer irrigation. This IMB method was designed to get theimmunoblot results more rapidly and clearly than the previous immunoblot ones. This study is aimed to evaluate the analytical accuracy of IMB using different biological assay. In the sensitivity test of IMB the monoclonal antibody can clearly detect the 30 ng (about 12 pM) of Mucocidin peptide (35 mer), and is also available to detect at least 10 ng (about 4 pM) of Mucocidin peptide (35 mer). The IMB was effective in the quantitative analysis of methothrexate (MTX) assay for cellular apoptosis. And more, this IMB is useful to screen large number of specific samples with ease and accuracy in a short time. In the screenings for the presence of Mucocidin in saliva the quantitative comparison is conspicuous among 48 persons depend on the different conditions ofgender, drinking and smoking habits, and oral diseases. Therefore, it is presumed that, even though the target proteins were partly degraded, a specific epitope can be detected if a monoclonal antibody was still reactive. Conclusively, these data suggest that the IMB can be useful in the primary qualitativeand quantitative analysis of proteins in various fluids, i.e., blood, saliva, tear, urine, etc.

Aspergillus niger CAD 1의 β-galactosidase를 알긴산나트륨에 고정화하여 갈락토올리고당을 생산하였다. 고정화 β-galactosidase의 열 안정성은 20∼45℃ 및 pH 안정성은 4.0∼5.5를 나타내었다. 고정화 효소의 활성화 에너지는 13, 400cal/mole이었다. 알긴산나트륨에 0.12unit/g의 β-galactosidase가 고정화 되었을 때 20%의 유당을 함유한 치즈 유청에 대한 갈락토올리고당의 수율은 45℃에서 반응 72시간 후 18%를 나타내었다. 10회 재사용하여도 고정화 β-galactosidase는 87%의 활성을 유지하였다.

교통사고로 인한 척추골절 환자 S75에서 손상부위에 따른 진단방사선학 및 보존적 치료 소견을 임상 문헌과 함께 고찰해 본 결과 다음과 같은 결론을 얻었다. 1. 흉요추 좌측방 단순필름에서 L1 요추에 설상변형 정도가 약 10%인 쐐기형 압박골절이 확인되었다. 2. L1 요추의 CT 촬영 결과 추체부 전면에 궁형의 불규칙한 골 절편을 관찰할 수 있었으며, T12∼L1는 구조상 손상빈도가 높은 부위로 밝혀졌으며, Denis의 골절형태는 중간주와 후방주가 잘 보존된 전방주의 다발성 압박골절로 분류되었고, Cobb의 척추 만각증은 없었으며, Frankel의 신경손상 분류형태는 E형이었다. 3. 정형외과적 치료방법은 비수술 요법의 보존적 치료를 원칙으로 하였고, 수분·전해질, 영양 및 혈장제 대용으로 공급하는 수액과 함께 비스테로이드성 항염진통제, 근육 이완제를 투여하면서 철저한 침상안정을 유도하였다. 4. 수상후 3주째는 폴리에틸렌 back corset를 착용하고 조기재활을 시도하였으며, 수상 8주 후에도 경도의 불편감이 남아 있었으나, 추시 관찰한 결과 일정기간이 지나면서 점차 척추는 안정성을 찾게 되었다.

Nepal産인 Nepal pseudo ginseng(NPG)의 大根과 小根 2종에 있어서 물추출 성분과 ethanol 침전성분이 암독소(Toxohormone-L)가 유도하는 지방분해에 미치는 영향을 관찰하고자 본 연구를 시도하였다. 지방질 분해인자로 알려진 독소호르몬-L은 Sarcoma180痛생쥐의 복수증액으로부터 부분정제하여 이용하였다. 암독소가 유도하는 지방분해 저해능은 반응의 최종농도가 500 및 1, 000 dalton 일 때 大根의 NPG은 ethanol 침전성분(평균 55.5%)이 물추출 성분보다 평균 1.37배 더 높았으나 小根의 NPG는 반대로 물추출 성분(평균 55.5%)이 평균 1.14배 더 높았다. 한편 대근의 NPG의 ethanol 침전성분이 지방분해에 미치는 저해능(47.6%)을 최종 반응농도가 1, 000㎍/㎖일 때 고려홍삼의 ethanol 침전성분(79.8%)에 비교하면 약 60%에 불과하였다.

This study aims to assess forest healing programs to middle-aged people in Korea with metabolic syndrome as a method to control the syndrome through prevention and health improvement rather than treatment. In order to develop healing programs in the urban forests for metabolic syndrome patients, environment condition of the forests and moods of participants were compared. Thermal environments and the concentration of phytoncides were analyzed by the site. Saneum Healing Forest had a lower temperature but a higher humidity than Seoul Forest. Seoul Forest had higher PMV and PPD levels than Saneum Healing Forest, providing patients with freshness. This seems to be due to the seasonal factor of autumn. As for the total emissions of phytoncide, mountain forest generated more than urban forest. Nine components out of investigated twenty turned out to be generated more in the urban forests. The atmospheric composition of phytoncides, volatile organic compounds that are released from vegetation, was analyzed at both sites. Profile of Mood States (POMS) was measured before and after the healing program. The POMS suggested that forest environments reduce stress and increase comfort, calm, and feelings of refreshment. The tendency towards positive mood state in the forest recommend that middle-aged Metabolic syndrome patients participate in healing programs in the forests.

Background : Bitter gourd (Momordica charantia) is a vegetable with pantropical distribution and contains the active ingredient, such as momordicine and charantin. Moreover, bitter gourd has been reported to exhibit antidiabetic, anticancer, cardiovascular-protective and antioxidant effects. But, the distinctive bitter taste of bitter gourd was not suitable for food preference. This study was conducted to evaluate the improvement effects of bitter taste of bitter gourd with natural fermentation and roasting process. Methods and Results : Bitter gourd fruits were obtained from Headeulnyeokae Co., Ltd.(Gang-Jin 59253, Repubilc Korea). Fruits of bitter gourd were prepared by natural fermentation process for 4h at 25℃. After natural fermentation, the fruits were dried in hot-air dryer for 15h at 49-55℃. The dried fruits were roasted in 500 g batches at 120℃ for 10 min in the electric rotisserie oven. After roasting, fruit pieces were extracted with hot water and cold water. One of them was cool-type, and the other was hot-type bitter gourd tea. In the sensory evaluation, hot tea and cold tea showed the high scores on color, flavor and overall acceptability. Conclusion : These mean that roasted bitter gourd had less bitter, and it could be utilized widely as drink and food material.