흰꿀풀은 꿀풀(Prunella vulgaris var. lilacina)의 근연종 중 하나로 꽃 색깔은 흰색이다. 자주색 꽃이 피는 꿀풀에 비해 드물다. 원산지는 한국으로, 어린순은 나물로 먹으며 꽃차례 째 그늘에 말린 하고초(夏枯草)는 임질·결핵·소염·이뇨·종기 등에 쓰이는 약재이다. 꽃은 꿀이 많아 밀원식물로도 쓴다. 섬초롱꽃은 경상북도 울릉군에 서식하고 있는 쌍떡잎식물 초롱꽃목 초롱꽃과의 여러해살이풀로 일반적인 초롱꽃이 백색을 꽃을 피우는 반면 섬초롱꽃은 연한 자주색을 꽃을 피운다. 우리나라 울릉도에만 살고 있는 특산식물로 밝혀졌다. 전초를 자반풍령초라고 하며, 청열, 해독, 지통의 효능이 있고 인후염과 두통을 치료하는데 사용하였다. 흰꿀풀과 섬초롱꽃의 내염성을 조사하고자 PK 비료처리구에 NaCl을 처리하여 생육을 비교한 결과 흰꿀풀의 경우 엽수의 감소가 뚜렷하였고, 섬초롱꽃은 엽수의 감소는 없었으나 전반적인 생육감소 현상이 나타났다. 앞으로 생리적 변화 양상을 추가 실험할 예정이다.

산국은 개국화라고도 하며, 산국 꽃은 진정·해독·소종 등의 효능이 있어 두통과 어지럼증에 사용한다. 관상용으로 심으며 어린순은 나물로 먹는다. 구절초는 구일초(九日草), 선모초(仙母草), 고봉(苦蓬)이라고도 불린다. 꽃이삭에 1.1%, 잎과 줄기에 0.01%의 정유가 있으며 전초는 알칼로이드 반응을 나타낸다. 항균성이 있고, 민간요법으로는 전초와 꽃 이삭을 해열, 폐렴, 기관지염, 기침, 감기, 인후염증, 방광질병, 무월경, 고혈압 치료 등에 사용했다. 본 연구는 산국과 포천구절초의 질소형태별 생육습성을 조사하여 자생식물의 활용도를 증진시키기 위하여 실시하였다. 배양액은 Hoagland 배양액을 변형 처리하였으며, 질소비료의 형태를 NO3-N, NH4-N, NO3-N/NH4-N 혼용처리구로 구분하였다. 비닐하우스에서 EBB 시스템을 이용하여 재배하였으며, 60일 후 초장, 엽수, 엽장, 엽폭을 측정하였고, 엽록소 함량을 조사하였다. 산국과 포천구절초 모두 초장, 엽장, 엽폭의 경우 NO3-N와 NH4-N 혼합처리구에서 가장 많았으며, NH4-N처리구의 엽수감소가 뚜렷하였다. 엽록소 함량은 산국은 처리 간 큰 차이가 없었으나 포천 구절초는 NO3-N, NH4-N, 혼합처리구 순으로 증가하였다.

꽃범의꼬리와 접시꽃을 공시재료로 하여 질소형태별 생육습성을 조사하여 자생식물의 활용도를 증진시키기 위한 기초조사를 실시하였다. 배양액은 Hoagland 배양액을 변형 처리하였으며, 질소비료의 형태를 NO3-N, NH4-N, NO3-N/NH4-N 혼용처리구로 구분하였다. 비닐하우스에서 EBB 시스템을 이용하여 재배하였고, 60일 후 초장, 엽수, 엽장, 엽폭을 측정하였고, 엽록소 함량을 조사하였다. 꽃범의꼬리는 초장, 엽장, 엽폭, 엽수 모두 NO3-N와 NH4-N 혼합처리구에서 가장 많았으며, NO3-N보다 NH4-N처리구가 초장 및 엽록소 함량이 높았으나 엽수는 더 적었다. 접시꽃은 NH4-N처리구의 생육이 현저히 감소하였으나 엽록소 함량은 큰 차이는 없었다.

Effects of nitrogen (N), phosphorous (P) and potassium (K) on the shoot and bulb growth of wild garlic (Allium victorialis var. platyphyllum) were studied by adopting in vitro culture. These macronutrients influenced the growth of both the shoot and bulb of garlic depending upon their application doses. A minimum of 3% potassium nitrate (KNO3) as a source of nitrogen was found to be critical for shoot elongation while higher concentrations were inhibitory. Garlic bulb growth was profuse on the usual KNO3 strength and sucrose (7%), followed by KNO3 (9.4 mM) supplement. On providing 41.22 mM ammonium nitrate (NH4NO3) as nitrogen source highest shoot growth was observed while 82.45 mM NH4NO3 as a source of nitrogen supported high bulb growth. With regard to potassium a good shoot growth was observed in medium that contained 0.31 mM KH2PO4 and 3% sucrose, while bulb growth was high on 2.5 mM KH2PO4 and 7% sucrose. These experiments may thus direct the development of excellent growth conditions for the commercial production of edible wild garlic.

This study was conducted to examine effects of plant growth regulators and light resources on the formation of multiple shoot and plant regeneration of Hovenia dulcis var. koreana Nakai. Stem and shoot tip were cultured on MS medium or WPM supplemented with various plant growth regulators. At the single treatment, the highest shoot formation was obtained when stem explants were cultured on WPM supplemented with kinetin 1.0mg·L-1. MS medium containing NAA 0.1 and TDZ 0.1mg·L-1 gave the best results for shoot induction rate and shoot growth in combination treatments. Of the BAP and kinetin tested, BAP 0.5mg·L-1 on WPM was found to be more effective for shoot growth from shoot tip. Under white fluorescent light treatment, shoot growth was much higher than blue, red LED treatments. Root induction from in vitro growth of plantlet was the best on WPM supplemented with 1.0mg·L-1 IBA. The results suggest that selection of plant growth regulators and light resources could be important factor to achieve an efficient in vitro growth.

An experiment was conducted to find out the effect of brushing treatment during cultivation on the postharvest quality of the baby leaf vegetable, specifically tah tasai Chinese cabbage (Brassica campestris var. narinosa). The effect of mechanical brushing during cultivation on the postharvest quality was determined in terms of the quality changes in weight loss, gas partial pressure, leaf color, and appearance during storage using a 30-㎛-thick polypropylene film at 16℃. The brushing treatment included brushing with A4 paper back and forth 50 times a day. The study revealed that the growths on the brushing-treated plant group were less than those on the control group. The structure of the leaf tissue of the brushing-treated plant also tended to be less compact than that of the non-treated plant. The brushing treatment resulted in less growth and denser plant tissues as well as in differences in the gas O2 consumption and CO2 accumulation after packaging. For the gas partial pressure, the O2 consumption and CO2 accumulation of the brushing-treated plant tended to be less than those of the non-treated plant. There were no differences, however, between the brushing-treated plant and control groups in the SPAD value and appearance. The study results also suggested that after packaging, the effects of the brushing treatment during cultivation on the quality of the tah tasai Chinese cabbage baby leaf vegetable was not significant. As such, it is recommended that effective post-harvest methods of improving the product quality of the baby leaf vegetable be further investigated.

The effect of the packing methods for enhancing the shelf life and improving the postharvest quality of the tah tasai Chinese cabbage baby leaf vegetable was studied during storage. Fresh baby leaf vegetables were packed in four commercial packaging types: (1) a non-perforated bag with a 0.03-mm oriented polypropylene (OPP) film; (2) a perforated bag with 1.0-mm-diameter holes on an OPP film; (3) a 0.40-mm polyethylene terephthalate (PET) container with a hinged lid; and (4) an expanded polystyrene (EPS) tray wrapped with a 0.02-mm polyvinyl chloride (PVC) film. The quality parameters, such as the weight loss, moisture content change, color difference, and appearance of the baby leaf vegetables were investigated. The baby leaf vegetables in the PET container and in the non-perforated OPP film bag showed relatively low weight loss, high moisture content, and good external appearance compared to those in the EPS tray and in the perforated OPP film bag during limited storage periods, at 16oC.ThePETcontaineralsoprotectedthebabyleafvegetablesfromphysicaldamage.Thestudyresultswillenabletheselectionofabetterpackagingsystemforextendingthefreshnessandincreasingthemarketabilityofbabyleafvegetables.

Investigation on oriental melon was carried out for 30 minutes starting at 7 PM every day from March 21 to May 24 to find out the effect of light emitting diode on seedling quality, grafting, growth and flowering of oriental melon. According to the result of the investigation, plant height was longer in Blue, Infrared, Red+Blue and Red treatment and leaf number was higher in Blue, Red+Blue and Infrared treatment than those of control. No big difference was identified between control and Yellow, Green, Ultraviolet treatments. Grafting rate was high in Green, Red+Blue and Green treatment. The number of flower evef}' week in control was nine, the number was almost 1 higher in White and Ultraviolet A treatments, but it was 1 to 4 lower in the rest of treatments. The number of female flowers of control was 10, however, it was 21 in Infrared treatment, 17 in White, 15 in Ultraviolet, 13 in Red+lnfrared, 12 in Blue and Red+Blue, 11 in Yellow and 8 in Green.

This experiment was carried out in order to collect the basic data on the standardization of the manufacturing process of Rehmannia glutinosa Libosch. var. purpurea Makino drying. By the drying methods of R. glutinosa, the content of water, inorganic components, reducing sugar, catalpol and benzo[α]pyrene were investigated. The water content was 15.6~17.2% when R. glutinosa was dried by cold-warm air moisture absorption drying method (CAMAD) at 60℃ during 6 days. Among of the inorganic components of R. glutinosa the K content was the most followed by P, Na, Ca and Mg. The reducing sugar content of R. glutinosa by the hot air drying method (HAD) was much more than that by the CAMAD. The catalpol content of R. glutinosa was not different by the drying temperature when it was dried by the CAMAD. The catalpol content of the large size tuber (about 50.0 g/unit) showed a tendency to increase from 60℃ until 70℃ drying temperature, but that of the small size tuber(about 4.0 g/unit) was decreased as being a trend as the drying temperature high when R. glutinosa was dried by the HAD, But the catalpol content R. glutinosa had a tendency to drop significantly at drying temperature above 80℃. The benzo[α]pyrene content was little detected when R. glutinosa was dried by both the SLD and the CAMAD, and the sampling by the HAD indicated within the scope of 5 μg/kg which was the scope to regulate by Korean food and drug administration. In conclusion, it seemed that an appropriate drying temperature of R. glutinosa by the CAMAD and the HAD was about 60℃ and about 70℃, respectively, when we consider the catalpol content and benzo[α]pyrene detection in the manufacturing process of drying R. glutinosa.

The potential antioxidant activities of different fractions from Prunella vulgaris var. lilacina were assayed in vitro. Among several fractions, n-BuOH fraction showed the highest 1,1-di[henyl-2-picrylhydrazyl (DPPH) free radical scavenging (IC50=0.50μg/mL). The results of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay showed the concentration dependency and n-BuOH fraction appeared a better result than the other fractions at the same concentrati on in this study. Moreover the total phenol and flavonoid contents of n-BuOH fraction contained the highest level. Additionally, correlation analysis indicated a high correlation between the antiradical activity and the total phenolic and flavonoid contents (p 〈 0.001). It suggests that n-BuOH fraction obtained from the 70% EtOH crude extract of Prunella vulgaris var. lilacina has wide potential for use as a source of antioxidant material.

적색 과육 '홍양' 품종에서 차등발현하는 유전자를 찾기 위하여 mirror orientation selection (MOS)과 결합된 suppression subtractive hybridization (SSH) 실험을 수행하였다. 그 결과, 288개의 cDNA clone을 확보하였으며, colony PCR을 통해 192개의 positive clone을 선발하였고, 이들을 sequencing하였다. NCBI/Genbank 데이터베이스의 BLAST 검색를

본 연구는 멀칭재료와 재식방법에 따른 shallot의 생육에 미치는 영향을 조사하였다. 멀칭비닐 종류별 시험에서 투명 PE필름 처리구에서 흑색 PE필름보다 엽수, 초장, 엽초장, 엽초경이 더 좋았고, 상품수량도 21%나 증수되었다. 그러나 구의 크기별 분포에서는 유의적인 차이가 없었다. Shallot의 재식밀도별 수량은 120 cm 5조(10 a당 20,833주)에서 1,332 kg으로 120 cm 2조(10 a당 12,500주)에서보다 1.7배가 증수되어 밀식재배가 유리하였다. 따라서 shallot의 재배는 투명 PE필름 멀칭에 휴폭 120 cm에 5조가 좋을 것으로 사료된다.

To characterize phytonutrients, the seeds of 12 purple Perilla (Perilla frutescens var. crispa) accessions collected from Korea and Japan were used for quantitative analysis of tocopherols, phytosterols, squalene and fatty acids. The average tocopherol, squalene and phytosterols contents were 12.2 mg 100g-1 , 3.99 mg 100g-1 and 77.20 mg 100g-1 , respectively. Among 4 tocopherol (T) isomers (α -T, β -T, ~gamma -T, and δ -T), ~gamma -T was present in the highest quantity (11.03 mg 100g-1 ) with the least variation (CV = 13.7%), while β -T was present in lowest quantity (0.25 mg 1100g-1 ). Compared to campesterol (4.36 mg 100g-1 ) and stigmasterol (13.32 mg 100g-1 ), β -sitosterol exhibited higher quantity (59.51 mg 100g-1 ) with 9.5% of variation. The major fatty acids were unsaturated fatty acids such as linolenic (61.5%), linoleic (17.3%), and oleic (9.9%) acids compared to saturated ones: palmitic (7.6%) and stearic (3.7%) acids. When Korean and Japanese accession were compared, almost no difference in content could be observed, while more variation as evaluated by CV (%) could be observed in Japanese accession in most phytonutrients suggesting wider genetic variation of purple Perilla in Japan. Presence of all above-mentioned phytonutrient compounds strongly suggested health beneficial value of purple Perilla seeds.

The demand of packaged baby leaves has been increased for its convenient use as fresh-cut produce. This investigation was aimed to explore the effects of different sanitizers on the quality parameters of 'Tah Tasai' Chinese cabbage (Brassica campestris var. narinosa) baby leaves. Thirteen days old baby leaves were harvested and washed in tap water (TW), 100 ppm chlorine solution (Cl), 2 ppm ozonated water (), 15 ppm chlorine dioxide solution () and washing with 0.2% citric acid solution followed by 50% ethanol spray (CA+Et). The samples were then packaged in 50 polyethylene bags and stored at for 10 days. Off-odor of packaged baby leaves was not detected during storage. There was no significant difference in color parameters among the treatments. Samples treated with showed substantially higher electrolyte leakage throughout the storage. This treatment also rendered a higher accumulation of in the packages. Samples treated with Cl and CA+Et maintained good overall visual quality with higher scores compared to that of and . Although Cl treatment showed lower number of total aerobic count at the beginning of storage, citric acid in combination with ethanol treatment was more effective until the end of storage. The combined treatment also showed comparatively lower coliform plate count. This result indicates that citric acid wash followed by ethanol spray could be an alternative to chlorine for environment friendly sanitization of baby leaves.

합성항산화제의 독성과 부작용으로 인한 천연물유래 항산화제에 대한 관심과 연구개발이 활발한 가운데 추출물 단계에서 ORAC assay를 통하여 우수한 항산화 활성을 가지는 쉬땅나무(Sorbaria sorbfolia var. stellipila Max.) 열매의 생물학적 생리활성 성분을 분리, 동정하기 위하여 국내에 자생하는 쉬땅나무의 열매를 채집하여 80% MeOH로 추출한 후 일반적인 용매분획법에 의해 분획한 분획물과 추출물을 대상으로 ORAC assay를 실시한 결과 80% MeOH추출물과 EtOAc, n-BuOH분획에서 천연항산화제인 trolox보다 우수한 활성이 나타났으며, 이중 가장 우수한 활성을 나타낸 EtOAc 분획물로 부터 각종 chromatography 기법을 통하여 분리, 정제한 결과 flavonoid계열의 화합물인 catechin을 단리 하였다. 단리 되어진 catechin은 쉬땅나무의 열매에서는 처음으로 본 연구에서 보고되는 성분으로 ORAC assay에서 대조군인 trolox에 비해 높진 않지만 유사한 활성이 나타나, vitamine E와 유사한 항산화 활성을 나타내는 것으로 보여지며, 따라서, 이 성분 외에 다양한 항산화 활성 성분들의 복합작용으로 인하여 쉬땅나무열매 추출물 및 분획물이 vitamine E에 비하여 3배 이상의 높은 항산화 활성이 나타나고 있음을 시사하는 바이다. 현재까지 쉬땅나무에 대한 식물학적 성분연구 및 유효활성 성분연구가 다른 천연물에 비하여 상대적으로 활발하게 이루어지지 않았으나, flavonoid계 화합물이 TLC확인 시험을 통하여 확인한 바 다양하게 존재하고 있어, 향후 이와 유사한 다양한 flavonoid계열의 화합물을 분리하여, 우수한 항산화 활성을 갖는 천연물의약품의 기초자료 제공 등 항산화 성분연구에 많은 기여를 할 수 있을 것으로 판단된다.

This study was carried out to investigate whether Chrysanthermum zawadskii var. latilobum Kitamura (C. zawadskii) extracts has an inhibitory effect against the mutagenicity by cigarette smoke condensates (CSC). C. zawadskii was extracted with 70% ethanol and the yield was 18.5%. We further fractioned 70% ethanol extract sequentially to diethylether, chloroform, dichloromethane, and aqueous water, and gained the yield of 17.5%, 5.6%, 5.8%, 32.8% and 35.5%, respectively. In the Ames test, there was no mutagenic effect of crude extract and its solvent fractions up to 2 mg/plate toward Salmonella typhimurium TA 98 with or without S-9 mix metabolic activations. On the contrary, the crude extract showed an inhibitory activity against the mutagenicity of CSC in the presence of S-9 mix metabolic activation. Diethyl ether layer among five solvent fractions showed the highest inhibitory activity. The inhibitory activity of diethyl ether fraction was also increased in a dose-dependent manner and the inhibitory rate was about 97.7% at the concentration of 1 mg/plate. In this study, we conclude that crude extract of C. zawadskii itself is potentially safe for mutagenicity, and the diethyl ether fraction has an inhibitory effect against the mutagenicity of CSC.

This experiment was held to identify the germination feature of Papaver radicatum var. pseudoradicatum which has high economical value. Seeds were collected in the Mt. Changbai in China. To eliminate dormancy of the seeds, chilling treatment were carried out in the 5 ℃ refrigerator. 30 days, 60 days, 90 days, 120 days of chilling treatments were taken. After being Treated, seeds were under 15, 20, 25 ℃ of temperature and light, dark conditions respectively to see the differences in germination. Within many features, germination ratio of seeds and germination period were investigated. Also, features related to early growth were checked through the closer look into acrospire, growth of cotyledon of young plant. Over 30 days of chilling treatment was all effective. Especially, 120 days of chilling treatment was most effective. The seeds germinated well in the light conditions. 120 days of chilling treatment, 15 ℃, light conditions were found to be most effective conditions as 54.5% of the seeds germinated. The germination period was 4.29 days. 30 days after germinating, length of the aerial part had been 1.90 cm. But after 45 days, the length grew rapidly to 4.58 cm. Fresh weight of scales plant was also increased dramatically after 45 days. Therefore, transporting the plant 45 days after seeding judged to be good.

The investigation was made about distribution and ecological characteristics of Rhododendron mucronulatum var. mucronulatum, R. dauricum and R. schlippenbachii on Mt. Changbai in China. The Ericaceae plants are known to be highly ornamental and easy to cultivate. The results of investigations are like followings. The plant species diversity was the highest in habitats of R. mucronulatum var. mucronulatum, followed by those of R. schlippenbachii and R. dauricum. R. mucronulatum var. mucronulatum community was found in the northwest exposure areas of 300m in altitude. Corylus heterophylla, Carex humilis var. nana, Atractylodes japonica species also grew in the R. mucronulatum var. mucronulatum community. R. dauricum communities were widely distributed in the west exposure areas of 1226-1860m above sea level. R. schlippenbachii populations were found in areas of under 300m above sea level, which has dry soil. But humidity of air was relatively high in the place. Artemisia japonica, Lespedeza bicolor and Dactylis glomerata were found around the habitats of R. schlippenbachii. Phalanx type of plants were dominant near the habitats of Ericaceae plants as 61%. Further, there appeared higher plant life totalling to 72 taxa comprising 54 species, 16 varieties and 2 forma with 31 families and 59 genuses at the whole survey area. Among these, Compositae plants excellent in resistance to environment was surveyed the most, accounting for 30.0%.

새로운 노지 월동작물과 수출유망 작물을 개발하기 위하여 1995년도에 shallot을 도입하였다. 적응력 시험을 수행하는 과정에서 구 비대기의 고온에 의한 식물체의 조기 고사현상 및 습해 발생으로 수량이 점차 감소되었다. 또한 영양번식체로 세대를 진전시키는데 기인한 종구의 퇴화현상과 같은 문제점이 발생하였다. 이러한 문제점을 해결하기 위하여 연도별 생산성과 지대별 적응성에 따른 shallot의 재배방법을 개발하기 위해 수행하였다. 연도별 생산성은 1995년에는 평균 구중이 18.7 g, 10a당 수량은 4,113 kg이었다. 재배 2차년(1996년)의 평균 구중은 25.8 g으로 무거워졌으나 수량은 10a당 2,013 kg으로 48.9%로 낮아졌다. 재배 3차년의 평균 구중과 10a당 수량은 1996년의 결과와 유사한 경향을 보였다. 재배 4차년(1998년)의 평균 구중은 21.1 g으로 증가하였으나 수량은 10a당 1,246 kg로 도입 당시의 30.3% 수준으로 크게 낮아졌다. Shallot의 고랭지, 준고랭지, 평야지, 해안 지대의 지대별로 적응력을 시험한 결과 식물체 생육은 월동 전 후 및 생육성기인 4월까지는 해안 지대에서 생육이 왕성하였다. 하지만 수확기인 7월에 가까워질수록 준고랭지가 해안 지대에 비해 수량이 57%가 많았다.