The acrosome cap allows sperm to penetrate the egg membrane and produce male pronuclei within female chicken eggs, facilitating successful fertilization. Given this, it is important to establish practical methods for evaluating the integrity of the acrosome cap and thus the quality of the rooster’s sperm. There are several established methods for evaluating the acrosomes of mammalian sperm, but none of these methods are suitable for evaluating the acrosome status of rooster spermatozoa. Therefore, a simplified method for evaluating the rooster acrosome is needed. Here we evaluated the usefulness of CBB (coomassie brilliant blue) staining of the acrosome at concentrations of 0.04%, 0.08%, and 0.3% CBB solutions. Our data revealed a clear staining pattern for intact acrosome caps at 0.04% and 0.08% CBB but not at 0.3% CBB. This protocol revealed differences in acrosome integrity between fresh and frozen rooster sperm smears suggesting that CBB staining may facilitate easier semen evaluation in roosters. This protocol allows for the accurate differential staining of acrosome cap in rooster spermatozoa.
보호지역 중 국립공원은 생물다양성이 풍부한 곳으로 다른 곳에 비해 인간에게 제공되는 생태계서비스 혜택이 높은 편이다. 이러한 국립공원의 가치를 객관적이고 과학적인 데이터를 기반으로 관리하기 위해 생태계서비스 평가가 활용되 고 있다. 생태계서비스는 공급, 조절, 문화, 지지의 4가지 서비스로 분류되며, 본 연구에서는 지지서비스 중 서식지질을 평가하였다. 서식지질 평가는 InVEST의 Habitat Quality 모델을 활용하였으며, 선행연구 분석 및 국내 보호지역 실정을 반영하여 인자별 민감도 및 서식지질 초기값 계수를 재설정하였다. 한라산국립공원을 제외한 21개 국립공원의 서식지 질을 분석하고 지도화하였다. 서식지질은 0과 1사이의 값으로 나타나며, 1에 가까울수록 자연성이 높은 것으로 평가한 다. 서식지질 분석결과 설악산·태백산국립공원(0.90), 지리산·오대산국립공원(0.89), 소백산국립공원(0.88) 순으로 높 게 나타났다. 해안-해상형 국립공원을 제외한 18개 국립공원의 면적과 서식지질을 비교한 결과 면적이 넓을수록 전반적 으로 서식지질이 높게 나타났다. 용도지구별 서식지질을 비교한 결과 공원자연보존지구, 공원자연환경지구, 공원문화 유산지구, 공원마을지구 순으로 서식지질이 높게 나타났다. 서식지질 분석과 용도지구별 법적인 규제를 함께 고려하였 을 때, 인위적인 행위가 제한될수록 서식지질은 높아지는 것으로 판단된다. 본 연구는 국내 보호지역 상황에 맞게 매개변수를 조정하여 21개 전 국립공원을 대상으로 서식지질 분석을 실시한 것에 의의가 있다. 적확한 수치와 지도화를 통해 직관적으로 파악이 용이하며, 향후 보호지역의 개발 및 보전에 관련한 정책 결정에 유용할 것으로 기대된다.
In this study, a study was conducted to improve the reliability of the valve by developing a valve leakage and reliability measurement system designed to secure the high quality and reliability of the butterfly valve. The system measuring the torque required for valve opening and closing operation, and was configured to operate after multiple opening and closing of the valve to check for leakage of the valve. Finally, a system that can perform efficient work in terms of productivity was developed by enabling leak inspection, torque measurement, and reliability inspection through one integrated system.
본 연구는 플라스틱 재료를 사용한 유공관 암거의 품질성능시험을 통해 간척지 농지 범용화에 적용가능 여부를 판단하였다. 직경 30mm, 50mm, 55mm의 폴리에틸렌 재질의 유공관을 사용하였으며, 품질성능평가 항목은 밀도시험, 인장강도시험, 충격강도시험, 투수시험을 실시하였다. 밀도시험결과는 모든 시험편에서 유공관 품질기준인 밀도 0.94g/㎤이상의 결과를 보였으며, 인장강도 시험은 직경 30mm와 직경 55mm는 24.5MPa이상으로 품질기준을 만족하지만, 직경 50mm 유공관에서 24.4MPa로 품질기준인 24.5MPa에 미흡하므로 보강이 필요한 것으로 나타났다. 충격강도시험은 모든 시험편에서 파괴도 5미만으로 품질기준을 만족하는 결과를 보였으며, 내면에도 이상이 없는 것으로 분석되었다. 투수시험 결과는 모든 시험편에서 품질기준인 0.1㎝/sec 이하를 만족하는 결과를 보였다. 본 연구에 적용한 주름유공관은 간척지 범용농지 에 지하배수암거로 사용이 가능한 것으로 평가되었다. 하지만 제품의 공장생산 시 품질관리가 미흡할 소지가 있으므로 현장반입 전 사전품질 관리가 필요하다고 판단된다.
This study aimed to assess the pollution level in 13 crowded subway stations in an effort to understand the spatial and seasonal factors of Indoor Air Quality. The main measured items were particulate pollutants such as PM10 and PM2.5 and gaseous pollutants such as CO2, HCHO, Rn, TVOC, BTEX, and Styrene at concourses and platforms in the summer and winter periods. The influence of the draught created by the movement of the train was classified into lateral and island platforms, and the concentrations of PM by location (entrance, middle, and end) were statistically compared and analyzed. As a result, the concentrations of PM were confirmed high in the order of Platform > Concourse > Ambient air. In particular, in the case of platform PM10, the frequency exceeding the standard value (100 μg/m3) was 38.5% and the maximum concentration was 196.2 μg/m3. All gaseous pollutants were at lower levels than the standard, and the factors affecting CO2 and Rn were identified as the number of users and geological characteristics, respectively. The principal component analysis (PCA) demonstrated that PM was found to be a major indicator of the air quality management of subway stations. In particular, the concentrations at entrance and end areas in the lateral platform were about 1.4 times higher with regard to PM10 than in the middle area, and about 1.9 times higher with regard to PM2.5 due to the effect created by the draught produced by the movement of the train. Therefore, in order to manage PM in the platform area, a specialized management plan for places with particularly high PM concentration within the platform area is required. In addition, it is necessary to evaluate the effect created by the draught produced by train movement when selecting locations for measuring indoor air quality.
Building Equipment field is getting important recently which satisfies the requirement for pleasant living environment. But, the noise generated in the machinery room often violates the pleasant living environment of residents in complicated form of airborne noise and soild sound, its seriousness is increasing so that damage of noise can take large percentage of environmental complaints. So, soundproofing and insulation of sound which can minimize such noise and effective countermeasure in case of civil appeal are required, but research materials regarding building equipment are definitely insufficient domestically. In this respect, this study used Zwicker parameter which is applied by psychological and acoustic reactions according to sound quality, analyzed sound quality of 32 kinds of building equipment used in machine room, and analyzed correlation between dB(A) and PSIL which are items of Zwicker parameter and noise valuation based on that. As the results, if valuates altogether loudness and unbiased annoyance which have high correlation with other existing suggested valuating methods, it is considered that it can be utilized as basic data for more reasonable and accurate grasping characteristics of noise from building equipment and for building effective countermeasure in case of civil appeal.
In this study, spray-dried double emulsion loaded bromelain was applied to Japanese Spanish mackerel fillets to develop fish-type, elderly-friendly foods. Japanese Spanish mackerel, treated with spray-dried double emulsions, were stored at 4oC for 24 h. For the characterization of emulsion particles, the particle size, ζ-potential, and morphology were determined. The color, TBARS, VBN, TMA, hardness, and adhesiveness of fish fillets treated by emulsion were measured for physicochemical properties. All the particle sizes of spray-dried emulsion were decreased while their double emulsion structure was maintained. Spray-dried emulsion reduced the color difference of fish fillets and did not catalyze lipid oxidation and protein decomposition. Values of TBA, VBN, and TMA of all fish did not exceed 0.5 mg MDA/kg, 30 mg/100 g, and 10 mg/100 g, respectively, which are considered edible limits regardless of bromelain or emulsion application. Bromelain modified fish fillets' hardness and adhesiveness to be suitable for elderly food, although it was encapsulated in the emulsion. Thus, spray-dried fish oil W/O/W double emulsion-loaded bromelain can be applied as a fish tenderizer, and this study’s results can be utilized to develop elderly-friendly food.
PURPOSES : This paper presents a quality evaluation of Korean foamed asphalt, which uses the maximum expansion ratio and half-life method. The maximum expansion ratio and half-life method are used to determine the optimum water content to produce a foamed asphalt mixture. The foamed asphalt mixture according to determine an optimum water content with this method; the mixture quality was compared with hot mix asphalt mixture.
METHODS : For the foamed asphalt mixed design, the water content was determined in addition to the Marshall mixing design method. The water content was determined using the ratio of the maximum to minimum volume and the time for the volume to decrease to half of the maximum volume. We conducted stability, indirect tensile strength, tensile strength ratio, dynamic immersion, and absorption rate tests to compare the foamed and hot mixed asphalt mixtures. RESULTS : The foam asphalt mixture exhibited less performance reduction due to temperature change than the hot mixed asphalt mixture. Most of the two mixture types exhibited similar performance. In addition, both mixtures should use an anti-stripping agent to improve water resistance.
CONCLUSIONS : As a result of the laboratory test, the foamed asphalt mixture was able to ensure a similar performance to the hot-mixed asphalt mixture.
In this study, the effect of improving indoor air quality according to the installation of plants was evaluated in classrooms where students spend much time. The purpose was to prepare sustainable and eco-friendly measures to improve the indoor air quality of school classrooms. A middle school in Bucheon was selected as an experiment subject, and IAQ monitoring equipment based on IoT was installed to monitor indoor air quality. After measuring the basic background concentration, plants and air purifiers were installed and the effects of improving indoor air quality using plants and air purifiers were evaluated based on the collected big data. As a result of evaluating the effects of indoor air quality improvement on the installation of plants and air purifiers, the reduction rates of PM10 and PM2.5 in descending order were plant- and air purifier- installed classes, air purifier-installed classes, and plant-installed classes. CO2 levels were reduced in the classrooms with only plants, and increased in two classrooms with air purifiers. The increase in CO2 concentration in the classrooms with plants and air purifiers was lower than in those with only air purifiers.
Companies struggle to make their best products with high quality and service at a competitive price in global markets. However, customer needs and requirements keep changing with a variety of situations. Companies that face the changes can not stay the same and make an effort to adapt themselves to new circumstances. They would probably review the overall management system that is currently implementing to improve management efficiency. Among other things, quality might be considered to be a crucial element if they are manufacturing industries to be sustained in global markets. KSA (Korean Standards Association) is a government- affiliated organization under the Ministry of Trade, Infrastructure, and Energy. It is a Korean standards provider for quality and service industry. KSA confers national commendations for organizations, quality circles, artisans, QCEC (Quality Competitive Excellent Company), and the most honorable KNQA (Korean National Quality Award) every year. KSA established KNQA on the basis of Malcom Baldrige National Quality Award, Deming Prize, and European Quality Award. Research on quality awards shows that there are many similarities in the framework. Although KSA summarizes two factors for 13 evaluation indicators in the quality competitive excellent model of QCEC, the categorization is ambiguous to explain them according to earlier studies. We performed a deep analysis of foreign quality awards and background for KNQA and QCEC. We conducted a content analysis of KNQA and QCEC and matched evaluation items that were closely related. We proposed a quality competitiveness model with three factors, Technology, System, and Tools, summarizing 13 evaluation indicators in QCEC. Based on audit data for six years from 2012 to 2017 we carried out a confirmatory factor analysis for the proposed model by examining the model validity and fitness.
Worldwide plant market keeps maintaining steady growth rate and along with this trend, domestic plant market and its contractors also maintain such growing tendency. However, in spite of its external growth, win-win growth of domestic material industry that occupies the biggest share in plant industry cost portion is extremely marginal in reality. Domestic plant material suppliers are required to increase awareness of domestic material brand by securing quality and reliability of international standard through improvement of design quality superior to that of overseas material suppliers. Improvement of design quality of plant material becomes an essential element, not an option, for survival of domestic plant industry and its suppliers. Under this background, in this study, priority and importance by each evaluation index was analyzed by materializing plant design stage through survey of experts and defining evaluation index by each design stage and based on this analysis result, evaluation index of stage-gate based decision-making process that may improve design quality of plant material was suggested. It is considered that by utilizing evaluation index of stage-gate based decision-making process being suggested in this study, effective and efficient decision-making of project decision-makers would be enabled and it would be contributory to improve design quality of plant material.
When producing rice products, it is very important to select suitable raw materials. Therefore, in this study, the quality characteristics of 16 rice cultivars were quantified to determine the criteria for evaluating the machinability of raw rice. The L value, which can affect the color of porridge prepared from rice, was the greatest for Hwaseonchalbyeo (84.17). The water-binding capacity, related to water interaction, was high in Hyangcheola (113.2%), and water solubility was high in Shingil (22.3%). Dodamssal (42.3%, 70.7 RVU) and Hwaseonchalbyeo (4.7%, 27.8 RVU) showed lower final viscosity compared to the cultivars in which the amylose content was medium groups (16.4~21.2%, 173.6~277.2 RVU). Specifically, cultivars with high or low amylose content had a low viscosity. The characteristics of the distribution of raw rice quality data were confirmed through 11 histograms. Furthermore, amylose content vs. water solubility, water solubility vs. peak viscosity, and peak viscosity vs. final viscosity showed high correlations (r=0.542, -0.569, and 0.836 respectively, p<0.01), and clear cultivar discrimination by the standard error of the mean (0.765~10.811). In conclusion, amylose content, water solubility, and peak viscosity were considered the most suitable characteristics for the quality evaluation of raw rice.
본 연구에서는 건조 고춧가루의 가공 및 저장라인에서 구리의 이용가능성을 조사하였다. 구리와 스테인리스강 표면과의 접촉을 통한 고춧가루의 색도 변화를 평가한 결과, 55oC에서 2시간 구리 판에 처리한 시료의 명도가 다소 증가하였다. 구리와 스테인리스강 접촉에 의한 고춧가루 내 미생물 저해는 중온성의 호기성 세균보다 곰팡이 및 효모의 저해에 더 효과적이었고, 55oC에서 2시간 구리 접촉에 의해 곰팡이와 효모의 수는 0.64 log 감소하여 유의적인 효과를 나타내었다. 산화방지 활성은 구리와 스테인리스 강 접촉 고춧가루의 DPPH 라디칼 소거활성은 감소한 반면 ABTS 라디칼 소거활성은 증가하였다. 본 연구는 구리 또는 구리와 스테인리스강 등의 합금을 이용하여 고춧가 루를 포함한 다양한 분말 식품의 가공 및 저장 과정에 활용할 수 있는 가능성을 제시하고 있다.
This study examined the dietary behaviors and dietary quality of high school students according to the number of times they skipped breakfast. A total of 474 high school students (225 boys and 249 girls) residing in Incheon participated in this survey. The subjects were divided into the three groups according to the frequency of eating breakfast; Regualr (eating breakfast everyday, n=226), Irregualar (eating breakfast 1~6 times/week, n=143, and Skipping (skipping breakfast everyday, n=105). The dietary quality was assessed using a nutrient quotient for adolescents (NQ-A). A higher monthly allowance and a higher rate of working mothers were significantly associated with a higher frequency of skipping breakfast. A higher intake frequency of processed beverages, and street food was significantly associated with a higher frequency of skipping breakfast. Students who had a higher rate of breakfast skipping had significantly lower intake frequency of fruits, white milk, bean and bean products, and fish. The high frequency of skipping breakfast among high school students was associated with lower dietary quality, as illustrated by the low total NQ-A score and sub group score including balance, environment, and practice. This information can inform efforts to provide nutrition education program to promote breakfast consumption and guidelines to students who skipped breakfast frequently.