Red onion cultivar ‘Eumjinara’ with mild taste and early maturation was developed in 2013 by the Allium vegetable crop research team, National Institute Crop Science, Rural development Administration (RDA). ‘Eumjinara’ was originally selected from red onion breeding line, ‘IS1115’, It was preliminarily selected and designated ‘Mon21-31-3’ and advanced yield trial was performed from 2008 to 2009. Regional yield trial test was conducted in six regions from 2010 to 2012. Foliage morphology and leaf color were similar to those of a check cultivar, ‘Cheonjujeok’, but its shape was comparatively broad ovate. Foliage of ‘Eumjinara’ lodged seven days earlier than check cultivar on May 31 and the average bulb weight (234±49.1 g/bulb) was also higher than that of check cultivar. The new cultivar was relatively resistant to clod hardness and downy mildew infection in the field condition. Average yield of ‘Eumjinara’ in three–year the regional yield trials was 6,999 kg/10a. The total soluble solid contents and quercetin concentrations were 41.43 μg/g FW and 556.11 μg/g FW, respectively.

양파의 관행 수확과 저장 방식을 개선하여 벌크 상태로 수확하여 수송, 하역, 보관 및 저장할 수 있도록 톤백 및 와이어철제파렛트를 개발한 후 관행저장 방식과 벌크저장 후 저장성을 비교하고 그에 따른 노동투입시간 및 소요비용을 비교하여 양파의 산지물류비 절감 효과를 비교 분석하고자 하였다. 본 연구를 통해 개발된 톤백이 관행 톤백보다 길이방향 인장강도가 16% 높았으며, 개발한 와이어철제파렛트가 관행철제파렛트보다 약 10% 더 저장가능하고 차량 적재 효율도 2배 이상 향상되었다. 개발된 500 kg용 와이어 철제파렛트의 벌크저장과 관행 그물망 저장의 감모율에는 차이가 없었으나, 1,000 kg 이상 많은 양을 저장할 경우 와이어철제파렛트 벌크저장은 관행저장보다 감모율이 3.7% 높아 적합하지 않는 것으로 나타났다. 톤백을 이용한 벌크 수확이 관행 망 수확보다 노동시간이 50.1% 그리고 총 투입비용은 46.1% 감소할 수 있는 것으로 나타났다. 와이어철제파렛트를 적용한 벌크저장이 관행저장보다 총 저장비용이 28.8% 감소하였으며, 2016년 양파생산량 (1,298,749톤)의 30%를 와이어철제파렛트 벌크저장으로 대체할 경우 연간 183억 원을 절감할 것으로 추정된다. 따라서 현재 산지업체들의 와이어철제파렛트를 조기 도입하여 정착시키면 전국적으로 노동력 및 비용 절감에 상당한 효과가 있을 것으로 판단된다.

The objective of this study was to investigate the effect of hot water extract of Welsh onion root (HEWO) on growth of lactic acid bacteria and fermentative characteristics of yogurt. The physiochemical characteristics of HEWO such as pH, soluble solid, reducing sugar, total polyphenol content and DPPH radical scavenging activity were studied. The lactic bacterial count in brain heart infusion (BHI) broth with HEWO was about 1 log cycle higher than in control for 24 h at 37℃. The pH of yogurt prepared with HEWO (WY100) and 50% HEWO (WY50) was gradually decreased significantly but increased the viscosity of yogurt with increasing HEWO concentration during fermentation. The viable cells of lactic acid bacteria after fermentation for 24 h were 8.03 (control), 8.77 (WY50), 8.84 (WY100) log CFU/mL, respectively. The DPPH radical scavenging activity of yogurt increased with increasing HEWO concentration. Sensory quality of yogurt prepared with HEWO was higher than that of control. The pH and lactic acid bacteria of all tested yogurts decreased during storage for 10 days at 4℃ but lactic bacterial count of yogurt prepared with HEWO maintained 108 CFU/mL during storage. These results indicated the potential use of HEWO as a valuable resource to improve fermentation and functionality of yogurt.

본 연구는 과열증기처리 기술을 양파에 적용하여 불쾌취 를 저감화하고, 이를 통해 고품질 양파 가공품 개발 및 소재 화에 활용하기 위한 기초 자료를 확보하고자 과열증기처리 에 따른 양파의 휘발성 물질, 불쾌취 유발 물질과 항산화활 성, 조직감, 관능적 특성 등 품질특성을 분석하였다. 과열증 기처리에 의해 휘발성 향기성분 및 불쾌취와 매운맛의 지표 물질이 저감화됨을 확인하였다. 항산화활성 및 총 페놀성 화합물 함량은 다소 증가하는 경향을 보였으며, hardness, chewiness 등은 확연하게 감소하는 것으로 나타났다. 양파 추출액의 총 가용성 고형분 함량은 처리에 의해 전체적으로 증가하였으며 처리에 따라 기호도 점수가 높아지는것을 확인하였다. 결과적으로 과열증기 처리가 양파의 불쾌취 저감화에 효과적임을 확인할 수 있었다. 본 연구결과는 과 열증기 기술을 적용한 양파의 불쾌취 저감화 및 이를 통한 다양한 고품질 가공품 개발 및 소재화를 위한 중요한 기초 자료로 활용될 수 있으리라 판단되며, 과열증기오븐 처리 를 통해 불쾌취를 조절함과 동시에 생리활성능을 지니는 향기 성분 및 유용성분의 감소는 최소화하는 추가적인 연구 가 필요할 것으로 사료된다.

Male sterile line in onion is essential to the development of hybrid varieties. Onion male sterile line 'Wonye30005' was developed by Bioenergy Crop Research Center, NICS, RDA in South Korea in 2010. Lodging date of 'Wonye30005' is May 15th and it belongs to the early maturing type in Korea. We found the male sterile plants in ‘Hamasodachi’, early maturing open-pollinated variety, and the male-sterile plants were cross-pollinated with male fertile line, ‘M330’, the population of F1 showed 100% male sterility and the F1 plants were subsequently backcrossed for five generations. 'Wonye30005' was numbered as ‘MMS77’ in 2009 and the corresponding male parent is called MMSB77. 'Wonye30005' has round bulb shape (Bulb index, 97), cold resistance, and excellent storage quality and average bulb weight is 253g. Flowering date is May 25th and flower bud size is 84 mm in full bloom. The number and length of flower stalks per bulb are 4 and 134 cm, respectively. 'Wonye30005' has been filed in Korea Seed and Variety Service in 2010. Overall, 'Wonye30005' is promising male sterile line for early maturing type hybrid seed production and breeding program. (no 4673)

본 연구에서는 기능성 부여와 저장성이 증진된 국수를 개발하고자 양파분말을 국수에 첨가(0, 3, 5, 10%)한 후 품질특성과 저장성을 조사하였다. 양파 생면의 수분은 양 파분말 첨가량의 증가에 따른 차이는 없었으나 pH는 감소 하였다. 양파 생면의 조리 후 중량과 부피, 수분흡수율은 대조구와 양파분말 첨가군 간에 차이가 없었다. 색의 경우 생면은 양파분말 첨가량이 증가할수록 L값과 a값은 감소하 고 b값은 증가하였으나 조리면은 큰 차이를 나타내지 않았 다. 생면의 총 폴리페놀 함량과 DPPH radical 소거능은 양파 분말 첨가량이 증가함에 따라 증가하였으며 조리후에는 함량이 전체적으로 감소하였으나 생면과 동일한 경향을 나타내었다. 양파 조리면의 조직감의 경우, 경도와 씹힘성 은 대조구가 양파 첨가군에 비해 높게 나타났으나 탄력성은 대조구와 양파분말 첨가군 간에 차이가 없었다. 생면을 1 0℃에서 12일간 저장하면서 총균수를 측정한 결과 양파분 말 첨가군은 대조구에 비하여 저장성이 최소 6일 이상 연장 될 수 있었으며 그 중 양파분말 5% 첨가구가 가장 효과적인 것으로 간주되었다. 양파 조리면의 관능검사 결과 외관, 맛, 냄새, 조직감 및 전체적인 기호도는 대조구와 양말분말 첨가군 간에 차이가 없었다. 따라서 본 연구결과로 볼 때 양파분말을 국수에 5% 첨가함으로서 기능성 부여와 저장 성이 증진된 국수를 개발할 수 있으리라 생각된다.

For efficient introgression of the downy mildew resistance gene from a resistant cultivar into domestic breeding lines, molecular markers used for marker-assisted backcrossing (MAB) were developed in onion (Allium cepa L.). The resistance gene (Pd) was originally introgressed from a wild species, A. roylei, by interspecific hybridization, and the resistant gene was known to be positioned at the end of chromosome 3. Therefore, cDNA sequences of loci located at the ends of chromosome 3 of two linkage maps were obtained from a transcriptome database. Primer pairs were designed on exon sequences of eight loci. Among them, the PCR products of the i25255 locus showed length polymorphism between A. roylei and onions, and both large and small-sized PCR products were observed in the resistant cultivar. Sequence analysis showed that a 67-bp indel existed in the intron sequences. Based on this indel polymorphism, a simple PCR marker, designated DMR1, was developed. Analysis of diverse onion accessions showed that no accessions contained the A. roylei-specific marker genotype except for the resistant cultivar. These results indicated that the DMR1 marker was successfully tagging the A. roylei fragment harboring the downy mildew resistance gene, and the resistant cultivar was heterozygous for the resistance gene. After further analysis of multiple loci positioned at chromosome 3, a range of the A. roylei fragment introgressed in the resistant cultivar was determined in two linkage maps. On the basis of the range of the A. roylei fragment, three molecular markers used for recombinant selection in MAB were also developed.

Bulb onion (Allium cepa) is one of the second most widely cultivated and consumed vegetable crops in the world. During winter where the temperature can be as low, plant could get cold injury and limit the production of bulb onion. However, the genomic resources available for bulb onion are still very limited. To date, no studies about heritably durable cold and freezing tolerance were carried out in bulb onion genotypes using high-throughput sequencing technology was applied. We sequenced cold (2°C) freezing (-5 and -15°C) treated and control (25°C) samples of contrasting genotypes of A. cepa lines and obtained 4,52,194,370 total high quality reads. After de novo assembly reads were assembled into 54,047 genes finally generated with an average length of 1,331 bp. Based on the similarity search aligning all genes with known public non-redundant (NR) database, including Swiss-prot, KEGG and COG. Differentially expressed genes (DEGs) were investigated using FPKM method. Overall, 92,862 genes were differentially regulated in all libraries were identified. Additionally, increase our understanding of the DEGs, we performed GO and KEGG pathway enrichment analyses. Based on FDR<=0.01 value in cold freezing tolerant line candidate genes were selected and discussed. Finally 25 candidate genes were examined using qRT-PCR were differentially regulated and known to be associated with cold and freezing stresses. Moreover, in silico prediction of putative molecular marker 4,437 SSRs and 6,076 SNPs. Our study is the first to provide the transcriptome sequence resource of Allium spp., for cold and freezing stress. We identified large set of genes to determine its DEGs profile under cold and freezing condition using two different genotypes. These data provides a valuable resource of genetic and genomic studies of Allium spp.

Onion and other Allium vegetables have been valued since antiquity for their pungent flavor and aroma. Modern science has confirmed traditional benefits that the organosulfur compounds that impart flavor also confer significant human health benefits such as reduced blood clotting and antimicrobial properties. Glucose, fructose and sucrose comprises majority of onion bulb dry matter content. The sugars, pyruvic acid accumulation and transcript level of some transcription factors involved in the biosynthesis of high sugars and pyruvic acid. These profiles were compared with two different lines 36101 (early) and 36122(Late) of bulb onion (Allium cepa L.) growing under drought and photoperiod condition using High Performance Liquid Chromatography (HPLC) and Quantitative real time PCR using FT genes. We identified the gene AcFT4 was responsible for early and late bulb intiation in the onion lines. The cultivar lines 36101and 36122 were used to identify potential genes controlling pungency and sugar. The comparative analysis of two lines showed significant positive phenotypic and genetic correlations. Sugar and pungency profile showed significant difference between two lines. FT gene expression and pungency level was high in onion lines during drought stress. In this study, we proposed the biochemical characterization of two line and genes involved in the bulb formation were also studied. There is a correlation between sugars and pungency level during the drought stress. These results could be presumably used as useful information to obtain onion varieties rich in sugars and pungency.

추출방법에 따른 양파껍질 추출물의 품질 특성 및 생리활성 특성을 비교하였다. 환류냉각추출의 수율이 13.21%로 가장 높았고, 폴리페놀과 플라보노이드 함량은 가압가 열추출이 가장 높게 나타났다. DPPH 라디칼 소거능 및 ABTS 라디칼 소거능 또한 가압가열추출이 가장 높게 측정되었다. α-Glucosidase 저해 효과는 80.24%로 환류냉각추출이 가장 높은 저해 효과를 나타내었고, ACE 저해 효과는 27.05%로 저온고압추출이 가장 높았다. 아질산염 소거활성은 가압가열추출이 33.97%로 가장 높았고 다음으로 환류냉각추출이 32.47%로 높은 활성을 나타내었다. Ferrous ionchelating 효과에서는 저온고압추출이 54.73%로 가장 높은활성을 나타내었다. 이러한 결과를 종합해 볼 때 가압가열추출이 소재 활용가치가 높을 것으로 사료되며 천연 항산화제 및 기능성 증진을 위한 소재로 이용 가능할 것으로 판단된다.

본 시험은 유기재배에 적합한 양파 품종 선발을 통해 유기재배 시 발생할 수 있는 문제점을 보완하기 위해 수행되었다. 농가 선호도, 육성계통, 육종회사, 고정종, 교배종, 국내종, 외국종 등을 고려하여 15개 품종을 선발하여 시험을 수행하였다. 각 품종들은 저온생장성, 동해 저항성, 추대, 분구 등 생리장해에 대한 저항성, 수확기 도복률 및 상품 수량 등 5개 영역에서 평가되었다. 교배종에서는 e-조은, 카타마루, 팝, 아트, 신기한 등이, 교배종에서는 창녕대고, 서울대고, 천주중고 등이 유기재배에 적합한 품종으로 선발되었다. 비록 적합한 품종으로 선발되지는 못했지만, 선파워는 구 특성에서 좋은 결과를 보였다. 고정종, 교배종 간에는 저온생장성(엽초경), 수확기 도복률(5월 24일)을 제외한 전 영역에서, 국내종, 외국종 간에는 월동 후 결주율을 제외한 전 영역에서 유의한 차이가 발견되지 않았다.

양파 웅성불임 계통 ‘원예30006’은 2000년에 중생계통인 ‘F2-6’집단에서 웅성불임개체를 선발하여 자식계통인 ‘HMB’를 화분친으로 하여 교배하였다. 2002년에 유지계통을 선발하고 2010년까지 5회 여교잡을 하여 계통의 안정성을 확인한 후 ‘MMS66’으로 명명하고 2010년에 직무육성신품종 심사를 거쳐 2011년에 품종보호출원 하였다. ‘원예30006’의 초형은 직립이며 중생종으로 도복기는 5월 25일, 초장 56 cm, 엽수 8매, 엽초경 12.4 mm로 초세가 중간정도이며 엽초경이 가늘다. 구중은 184 g의 중구형에 속하며, 구형지수 99로 원형이다. 저장성과 내한성이 강하고 노균병에 다소 저항성이 있다. 채종모구의 개화기는 6월 4일, 화경수 4개, 화경장 115 cm, 화구폭 86 mm이며 웅성불임 발현이 안정적이다.

Flavonols as a major kind of plant secondary metabolites are known for health-promoting compounds in onions (Allium cepa L.). The objectives of this study are to determine profiles of flavonol glycosides in different 75 onion accessions. A total of five flavonols (quercetin 3,4'- diglucoside, Q34'diG; quercetin 3-glucoside, Q3G; quercetin 4'-glucoside, Q4'G; isorhamnetin 4'-glucoside, I4'G; quercetin, Q) were identified from onion accessions. In positive ion mode using LC-ESI-MS, individual flavonols were confirmed from one and two glycosylation binding with aglycone such as quercetin and isorhamnetin. Total flavonol contents were distributed in white onion (range of 0.18-6.47 mg/g DW) and purple onion accessions (range of 2.39-6.47 mg/g), respectively. The mean of flavonol contents in purple onion (4.41 mg/g) showed 1.4-fold higher than white onion (3.23 mg/g). The Q34'diG and Q4'G were considered as the major compounds of flavonol glycosides in onion accessions.

Fluorescence in situ hybridization (FISH) is a powerful tool for the detection of DNA sequences in the specific region of the chromosomes. As well as for the integrated physical mapping, FISH karyotype analysis has to be preceded. The detailed karyotypes of two onion cultivars, which are resources for onion genome sequencing project (‘Eumginara’ and ‘Sinsunhwang’), were constructed based on triple color fluorescence in situ hybridization (FISH) using 5S rDNA, 45S rDNA, and tandem repeat sequence. All used our materials showed 2n=2x=16 with x=8 as basic chromosome number. 5S rDNAs were located on 4 loci in one pair of interstitial region of short arm chromosome in both onion cultivars. Two pairs of 45S rDNAs were positioned in distal region of short arm chromosome in ‘Eumginara’. Otherwise 5 loci of 45S rDNAs were located in distal region of two pairs of short arm chromosome in ‘Sinsunhwang’. Among them, two signals of 45S rDNAs were co-localized in distal part of short arm and long arm chromosome, respectively. In case of tandem repeat sequence was detected on telomeric region of 8 pairs of chromosomes except on 45S ribosomal DNA sites. These results will provide a valuable background for physical mapping and help to further more understand the genome sequencing project in Allium cepa.

Bulb onion (Allium cepa), which belongs to the family Amaryllidaceae, is one of the oldest vegetative crops known to humans. Despite its high economic value, only a few reports are available on the use of molecular markers in genetic diversity analysis of Allium cepa for its improvement. Molecular genetic markers have been widely used as powerful tools for analyzing the plant genome. In particular, Microsatellites or simple sequence repeats (SSRs) markers are tandem repeats of one to six bp in length and have been proven to be the most powerful polymerase chain reaction (PCR)-based DNA markers in plant diversity analysis. In this study, the genomic DNA was isolated from different Allium cepa lines. The ESTs and gDNA sequences of onion were collected from National Center for Biotechnology information. The SSRs with two to five motifs over a length of 12 bp, were identified using SSRIT (Gramene) software. The PCR products of 100 to 350 bp in length containing SSRs, primers was designed using Primer3 with lengths of 20 to 24 bp and a melting temperature of 60℃. The SSR markers with high polymorphism-information content (PIC) levels was useful for collecting progeny with high genetic homogeneity for onion breeding, and to obtain representative marker sets for genetic tests. The SSR Finder program and the developed SSR markers could be a useful resource for genetic diversity and purity testing in onion.

Onion(Allium cepa L.) is one of the most important crops to the fresh vegetable spices and the food processing industry in Korea. To evaluate genetic variation in onion, major agricultural characteristics and biochemical characteristics such as flowering day, harvest day, brix and so on. Phenolic compound compositions were analyzed for 44 accessions. Accessions in white color was about 92% which is the largest one followed by standard criteria. The sweetness of juice ranged from 6.1 to 19.3(in brix), and Brix range of 117 accessions was from 10.1 to 14.0. High performance liquid chromatography(HPLC) was used to identify the phenolic profile and quantify phenolic content in bulbs: quercetin, quercetin 7, 4’-diglucoside, quercetin 3-glucoside and quercetin 4’-glucoside were detected as major components. The total Quercetin and Quercetin glycoside content ranged between 123.59 and 1155.84 mg/100mg bulb freeze dried weight. The quercetin contents was up to 16.7mg/100g, and 7.41mg/100g in average. It is expected that the result of this study can be used for breeding more competitive species with respect to contents in functional chemicals.

Onion (Allium cepa L.) is one of the richest sources of flavonoids in human diet. Onion peel contains over 20times more quercetin than onion flesh. In this study, we studied the effects of onion peel water extract (OPE) on the bloodlipid profiles in mice. The onion peel extracts was extracted with hot water. The experimental groups were divided with 3groups (n=6) of ICR male mice: normal diet+distilled water (NC), high-fat diet+distilled water (HF), high-fatdiet+onion peel water extract 20㎎/㎏ (OPE-20). The oral administration was conducted daily. The experimental periodwas 7 weeks. Onion peel water extract showed higher concentration of polyphenol gallic acid and anti-oxidant trolox equiv-alent than the ethanol extract. The body weight gain and food efficiency ratio was significantly lower in the OPE-20 group ascompared with HF group (p<0.05). The epididymal fat and retroperitoneal fat showed significantly lower weights and sizesin the OPE-20 group as compared with HF group (p<0.05). The serum levels of total cholesterol, LDL cholesterol and trig-lyceride were significantly lower in the OPE-20 group as compared with HF group (p<0.05). The OPE-20 group showedhigher HDL cholesterol concentration than HF group (p<0.05). Atherogenic index was ignificantly lower in as comparedwith HF group (p<0.05). The serum levels of glucose, GOT and GPT were significantly lower in the OPE-20 group as com-pared with HF group (p<0.05). In these results, we suggests that onion peel water extracts supplementation can reduces theserum lipid components and improves the lipid metabolism in hyperlipidemic mice induced with a high-fat diet.

양파를 첨가한 청국장 제조 가능성을 평가하고, 제조된 시료들을 대상으로 항산화능을 비교하였다. 먼저 양파의 적정 첨가량을 판단하기 위해 노랑콩과 검정콩 각각의 중량을 기준으로 0, 5, 10, 20, 30%가 되도록 양파를 첨가하여 청국장을 제조한 후 관능평가를 실시하였다. 그 결과, 노랑콩과 검정콩 청국장 모두 20% 양파 첨가 청국장이 높은 기호도를 나타냈다. 그래서 20%의 양파를 첨가하여 제조한 청국장을 대상으로 특정성분의 함량 및 항산화능을 비교ㆍ평가하였다. 그 결과, 양파를 첨가하여 제조한 청국장이 첨가하지 않은 청국장보다 유의하게 더 높은 총 페놀성 및 flavonoid 함량을 나타냈다. 그리고 DPPH radicalscavenging 활성평가에 있어서도 양파를 첨가하여 제조한 청국장이 첨가하지 않고 제조한 청국장보다 더 높은 radical-scavenging 활성을 나타냈다. 또 쥐 혈장 산화에 따른 CE-OOH 생성 억제능 평가에 있어서도 양파 첨가 청국장 추출물을 투여한 쥐혈장이 양파를 첨가하지 않은 청국장 추출물을 투여한 쥐 혈장에 비해 더 높은 산화 억제능을 나타냈다. 이상의 결과들은 양파에 다량 함유된 quercetin을 포함한 flavonoid들과 발효과정 중 생성되는 다양한 페놀성 화합물들에 의한 효과라 판단된다. 본 연구결과가 국산 양파의 가공시장확대 및 청국장 소비의 저변확대를 위한 기초 자료로 활용되길 기대한다.