The aim of this study was to investigate what protein(s) of porcine epididymal fluid (pEF) are able to enhance the nuclear maturation of porcine germinal vesicle (GV) oocytes in vitro. Proteins of pEF were fractionated by affinity, ion exchange, and gel filtration chromatography. Porcine cumulus-oocytes complexes (COC) from follicles were cultured in tissue culture medium (TCM 199) containing various fractions obtained by chromatography. Porcine COCs were also cultured in TCM 199 containing various meiosis inhibitors and pEF. After 24 or 48 h culture, oocytes were examined for evidence of GV breakdown, metaphase I, anaphase-telophase I, and metaphase II. When porcine COCs were cultured in the medium with meiosis inhibitor such as, dibutyryl cAMP (dbcAMP) and forskolin (Fo), more than 80% of oocytes were unable to resume meiosis. However, porcine COCs supplemented with pEF were able to overcome the inhibitory effect of dbcAMP and Fo. Maturation rate of oocytes was significantly (p<0.05) increased in the media supplemented with cationic protein(s) during in vitro maturation than in those with anionic protein(s) (44.1% vs 20.0%). When oocytes were cultured in the TCM 199 with fractions obtained by gel filtration, the maturation rate of oocytes was significantly (p<0.05) higher in fraction 11 containing 18 kDa than other fractions. The present study suggests that 1) dbcAMP and Fo prevent the spontaneous maturation of oocyte after isolation from follicles, and that pEF contain a substance(s) that improves meiosis resumption in vitro of porcine COCs, 2) cationic 18 kDa protein(s) are responsible for promotion of MⅡ stage.

본 논문에서는 유체 시뮬레이션을 이용한 후광 효과(halo)를 제안한다. 후광 효과는 주로 캐릭터의 강조를 목적으로 사용된다. 제안 방법은 기존의 2차원 이미지 편집 도구를 이용한 후광 효과 제작 방식과 유사하다. 그러나 2차원 이미 지에 가우시안 블러 효과를 적용하는 방식이 아닌, 3차원 캐릭터의 외곽선을 유체 방정식을 이용하여 확산시킨다는 점에서 차별성을 갖는다. 즉, 후광 효과에 유체와 같은 움직임의 부여가 가능하다. 실시간 계산 효율성을 위해서 3차 원 형상에서 실루엣 외곽선을 검출한 후 평면에 투영하고 2차원 유체 방정식을 적용하는 간접적인 방식을 취한다. 2 차원 결과 영상에 오목렌즈와 같은 이펙트를 추가하여 왜곡과 과장에 의한 입체감 있는 후광 효과가 가능하다. 제안 하는 방법은 매 프레임마다 캐릭터의 외곽선으로부터 새로운 후광 효과를 생성하기 때문에 게임과 같은 실시간 애니 메이션에 더욱 효과적으로 응용될 수 있다.

Diesel particulate matter is one of the major harmful emissions under strict regulations. The exhaust after-treatment devices are added to the diesel engines as stricter regulations are introduced worldwide. In the present study, new system of smoke reduction of diesel engine is proposed. This new system is using suction equipment and fiber glass filter included moisture for capture smoke. For verification of new system experiments are performed at diesel engine. As a result it is founded that smoke is decreased.

Recently, the Ranque-Hilsch vortex tube is widely used for the local cooler of industrial equipment for special purpose. Although many studies on energy extraction in the vortex tube using air as the working fluid have been made so far, a few experimental studies treated solid particles extraction for incompressible fluid. So, an experimental study for the solid particles extraction in the vortex tube(Ranque-Hilsch vortex tube) using the water which is essentially an incompressible fluid is presented. The experiments were carried out with various cold end orifice diameter ratios ranging from 0.25 to 0.70, the input pressure ranging from 1 to 3MPa was considered. The emphasis was given to examine the effect of geometry factors of vortex tube at working fluid(water) for solid particles(Al2O3) extraction. The optimum geometry factor and inlet pressure for the maximum solid particles extraction was found that the smaller cold end orifice diameter ratio and the higher inlet pressure in experimental condition increase.

본 연구는 고흥 남성리 갯벌양식장의 구조물 설치 및 양식장의 공간 배치를 위한 기초자료로 대상해역의 지형구조 및 해수 순환특성을 조사하였다. 관측결과 남성리 갯벌양식장의 경사도는 약 1도 미만으로 매우 평판한 것으로 나타났으며, 남성리 갯벌양식장 의 지형구조를 파악하기 위하여 비디오영상 자료를 이용하였다. 조사결과 남성리 양식장의 시설물은 굴패각 망태 또는 사석의 축제 형 식으로 총 5가지의 구조형식을 가지는 것으로 조사되었으며, 양식장은 지형구조상 노출 시간이 총 181분으로 약 3시간 동안 대기에 노 출되었으며, 침수된 시간은 약 434분으로 약 7시간에 해당되었다. 조사된 영상자료는 수치해도의 자료와 서로 보완하여 양식장의 지형 특성을 파악하는데 사용되었다. 대상해역은 나로도에 의하여 외해와 차폐된 해역으로 해수유동 수치실험을 통한 해수순환 특성은 북측 으로 정암면과 나로도 사이의 수로와 남측의 남성리와 나로도 사이의 좁은 수로를 따라 남-북방향의 주흐름을 가지는 것으로 조사되었 으며, 나로대교가 위치한 갯벌양식장 해역 전면의 수로에서 최대 1.5 m/s 의 흐름을 보이는 것으로 조사되었다.

This study investigated whether orthodontic force influences the production of osteoprotegerin (OPG) and receptor activator of nuclear factor-kappa B ligand (RANKL) in vivo, both of which are affected by cortical activation. Mechanical force was applied to the maxillary premolars of orthodontic patients by fitting the transpalatal arch prior to cortical activation of the gingival tissue. Gingival crevicular fluid (GCF) samples were then collected from each patient using paper strips before and after 1, 3, 7 or 14 days of treatment. The OPG and RANKL levels in the GCF were determined by enzyme-linked immunosorbent assays. The levels of OPG were significantly increased after 1 day of fitting the appliance and decreased to basal levels at 3 days after fitting. In contrast, the RANKL levels were dramatically decreased at 1 day after fitting, but recovered to those of the untreated control at 3 days after the force application. The force-mediated changes in the OPG and RANKL levels of the GCF were unaffected by cortical activation during these experimental periods. Collectively, these results suggest that an acute and severe change between the OPG and RANKL levels plays an important role in stimulating the cellular responses required for alveolar bone remodeling by orthodontic treatment.

The aim of this study is to investigate the effect of porcine epididymal fluid (pEF) on in vitro-maturation and subsequent fertilization of porcine follicular oocytes. Porcine cumulus-oocytes complexes retrieved from antral follicles were cultured in tissue culture medium (TCM)-199 supplemented with pEF of different concentrations. At 48 h after culture, development of oocytes to germinal vesicle (GV) breakdown, metaphase I, anaphase-telophase I, and metaphase II were examined. Significant (p<0.05) increase in the proportion of oocytes developed to MII stage was observed in oocytes cultured in pEF-containing TCM-199 than in oocytes cultured in pEF-free TCM-199 (46.2% vs 16.7%), which was a dose-dependent manner. Subsequently, the proportion of monospermic fertilization were significantly (p<0.05) increased in oocytes cultured in the TCM supplemented with pEF than those cultured in pEF-free TCM-199 (51.0% vs 24.1%). In the second series of experiment, the percentage of MII oocytes was significantly (p<0.05) increased after exposure of oocytes to pEF during the first 22 h period of culture than after exposure of oocytes to pEF during the next 24 h of culture, while no significant difference in the percentage of monospermy was observed. The results of this study demonstrate that pEF contains at least enhancing component(s) for nuclear maturation.

This study was conducted to examine the effects of human follicular fluid and gonadotropin (FSH+HCG+rhEGF) on in vitro maturation, fertilization and development of human immature oocytes. Cumulus-oocyte complexes (COCs) were collected following for in vitro fertilization and embryo transfer (IVF-ET) cycles of the patients. At the time of oocytes collection, oocytes were classified into MII, MI and GV in accordance with their appearance (MII: Fully mature oocyte at metaphase II of meiosis; MI: Nearly mature oocytes at metaphase I of meiosis; GV: Immature oocytes at prophase I of meiosis). After controlled ovarian stimulation using gonadotropin(FSH) and human chorionic gonadotropin (HCG) in 70 ICSI cycles, 158 MI to MII matured oocytes were intracytoplasmic sperm injection (ICSI) h after in vitro culture and 553 MII oocytes were ICSI after denudation. The aspirated MI and GV oocytes were cultured in culture medium containing 10% (v/v) serum protein substitute (SPS), 10% (v/v) human follicular fluid (hFF) and 10% (v/v) serum protein substitute (SPS)+1 IU/ml FSH+10 IU/ml HCG+10 ng/ml recombinant human epidermal growth factor (rhEGF). The maturation rate of immature oocytes was similar among the three group. When maturation medium was supplemented with 10% SPS, 10% hFF or gonadotropins, the fertilization rate of in vitro matured oocytes was higher in 10% SPS (80.0%), but there was no statistical significance (78.2%; hFF, 76.9%; gonadotropin, p>0.05). The development rate of human embryos developed to cells were not significant difference in the medium containing SPS, hFF and gonadotropins (65.6%, 65.9% and 66.7%). The results of these study suggest that human follicular fluid and gonadotropins supplemented in the culture medium was not effected on the in vitro maturation, fertilization and development of human immature oocytes.

This paper presents a novel single-step method to prepare the Ag nanometallic particle dispersed fluid (nanofluid) by electrical explosion of wire in liquid, deionized water (DI water). X-ray diffraction (XRD), field emission scanning electron microscope (FE-SEM) and transmission electron microscope (TEM) were used to investigate the characteristics of the Ag nanofluids. Zeta potential was also used to measure the dispersion properties of the as-prepared Ag nanofluid. Pure Ag phase was detected in the nanofluids using water. FE-SEM analysis shows that the size of the particles formed in DI water was about 88 nm and Zeta potential value was about -43.68 without any physical and chemical treatments. Thermal conductivity of the as-prepared Ag particle dispersed nanofluid shows much higher value than that of pure DI water.

Three-dimensional, statistical-mechanical formulations of problems are usually untractable analytically, and therefore they are commonly solved numerically. However, their one-dimensional counterparts are always to be solved analytically. In general analytical solutions sheds more insights to the problems than numerical solutions. Hence, solutions of one-dimensional problems may provide key properties to the problems, when they are extended to three dimensions. In this article, thermodynamic properties of one-dimensional fluid comprising molecules of rigid rods are analyzed statistical-mechanically. Molecules of rigid rods are characterized with repulsive or excluded volume effect. It is observed that this feature is well reflected in thermodynamic functions such as Helmholtz free energy. volumetric equation of state. chemical potential, entropy, etc.

The paper reports on the prediction of turbulent heat transfer in flows between parallel plates with wall transpiration. The elliptic blending second moment closure for turbulent stresses and the GGDH model for turbulent heat fluxes are employed to predict the turbulent flow and heat transfer. The numerical results by the adopted models are directly compared to the DNS data and the measurements to assess the performance of the model predictions. The predictions show correctly the effect of deceleration and acceleration of the flow caused by the transpiration, and the anisotropy of the turbulence structure is augmented towards downstream by the fluid injection. The turbulence structure and heat transfer characteristics of transpired flows are well captured by the present turbulence and heat flux models.

옥천습곡대의 서남부지역에서 분포하는 쥬라기 화강암류의 석영내에 존재하는 아문 미세균열과 유체표유물을 분석하여 이 지역에 작용한 고응력장을 해석하였다. 연구지역에서 나타나는 아문 미세균열의 방향성은 전체적으로 N30˚W의 방향이 가장 우세하며 N70˚W의 방향도 나타난다. 연구지역의 아문 미세균열 생성온도는 380-550˚C 범위를 보이며, 이들 아문 미세균열은 약 166-200Ma의 기간 동안 형성되었을 것으로 추정된다. 아문 미세균열의 방향성을 통한 고응력장의 작용 방향과 유체포유물에 의한 아문 미세균열의 형성시기를 비교하여 볼 때, 연구지역 내에서 발달하는 화강암질암체는 NNW-SSE와 WNW-ESE 방향의 최대수평주응력인 고응력장이 쥬라기 초기에서 쥬라기 중기 기간 동안 작용하였을 것으로 사료된다.

The aim of this study was to investigate what components of porcine epididymal fluid (pEF) influences the nuclear maturation of porcine germinal vesicle oocytes. Porcine cumulus-oocytes complexes from follicles were cultured in TCM 199 containing pEF. After 48 h cultures, oocytes were examined for evidence of GV breakdown, metaphase I, anaphase-telophase I, and metaphase II. Maturation rate of oocytes was significantly increased in media supplemented with 10% pEF during in vitro maturation (IVM) than in those without pEF. When lipid component of pEF was removed by treating n-heptane, no significant difference was observed in maturation of oocytes between n-heptane treatrment and intact pEF group. However, the proportion of oocytes reaching at metaphase II (M II) was significantly (p<0.05) decreased in the oocytes cultured in media containing trypsin-treated pEF compared to those in media with intact pEF. When porcine GV oocytes were matured in the medium supplemented with intact pEF or pEF heated at 56'C and 97'C, rates of oocytes remained at GV stage were 11.7%, 29.4% and 42.0%, respectively. However, there were no difference in proportion of oocytes reaching at MII stage among intact pEF group and 56'C group. Present study suggests that 1) pEF contains an enhancing component(s) for nuclear maturation in vitro of oocytes, 2) protein(s) of pEF may be capable to promote nuclear maturation in vitro, and 3) enhancing component for nuclear maturation may consist of two factors, which are responsible for germinal vesicle breakdown (GVBD) and promotion of MII stage.