Growth differentiation factor8 (GDF8) is a member of the transforming growth factor-β that has been identified as a strong physiological regulator. Overall of the current studies, the GDF8 is detected in oviduct fluid and uterus which led us to suggest that the GDF8 may effect on preimplantation embryonic development and act paracrine role to correlate with successful late-blastocyst implantation in in vivo. The purpose of this study is the effects of GDF8 on porcine parthenogenesis (PA) embryo development during in vitro culture (IVC). We were investigated the effect of GDF8 supplement during PA embryo IVC by cleavage and blastocyst formation rate and patterning analysis. Data were analyzed by on way ANOVA, followed by Tukey’s range test. Respectively 0.2, 2 and 20 ng/mL of GDF8 were added during IVC followed experiment design as control, 0.2, 2, and 20 GDF8 supplement groups. After 48h of embryo culture time, no significant difference was observed on cleavage rate from the different concentration (0, 0.2, 2, and 20 ng/ml) of GDF8 supplement groups (65.7%, 66.0%, 66.3%, and 65.8%, respectively). After 120h of embryo culture time, the 0.2 and 2 group showed significantly (p<0.05) higher blastocyst formation rate than control (40.4% and 36.4% VS 40.4%, respectively). In embryo developmental pattern analysis, the 0.2 ng/ml GDF8 supplement groups showed significantly higher (p<0.05) 2-3 cell cleavage- and early blastocyst pattern compared with control (12.0% and 10.4% VS 6.6% and 6.2%, respectively). However there are no significantly different pattern was observed in other groups. In conclusion, the 0.2 ng/ml of GDF8 supplementation during porcine PA embryo IVC significantly changed embryonic developmental patterns. However there are further studies are required such as analysis of blastocyst total number, specific gene transcription pattern, and ICM/TE rate to make clarify and support the conclusion.

본 연구는 서울시내에서 시판중인 식육에서 E. faecalis 를 분리하고 이 균들의 항생제 내성 패턴, 항생제 유출 펌 프 유전자 및 병독성 유전자의 분포를 분석하였다. 총 277 개의 식육시료에서 93균주의 E. faecalis 를 분리하였다. 이 균주들의 항생제 내성비율은 ampicillin에는 35.5%, chloramphenicol에 6.4%, ciprofloxacin에 4.3%, eryhtromycin 에 18.3%, quinupristin-dalfopristin에 76.3%, tetracycline에 45.2%의 내성이었으며 levofloxacin, teiconplanin 및 vancomycin에는 모든 균이 감수성이었다. 약물 유출펌프인 MFS 타입의 eme(A)와 ABC 타입의 efr(A)유전자는 모든 균주 (100%)에서 확인되었으며 efr(B)는 98.9%, lsa는 91.4%의 균주에서 확인되었다. 병독성 인자인 gel(E)는 68.8%, ace 는 90.3%, asa1는 47.3%, efaA는 91.4%, esp는 12.9%의 균주에서 확인되었다. 본 연구는 시판 식육에서 분리한 지 표 미생물의 하나인 E. faecalis의 항생제 내성, 약물유출 펌프 및 병독서 유전자의 분포를 분석한 연구로 지속적인 모니터링을 하여야 할 것으로 사료된다.

한국에서 말라리아는 1993년에 다시 등장했으며, 이후 증감을 반복하며 공간적 변화를 거듭하여 왔다. 지리 공간의 인문·자연·지리적 요인들은 복잡하게 상호작용하여 매개 모기의 생태와 말라리아 발생에 영향을 미친다. 본 연구는 말라리아의 발생 양상을 이해하고 이를 조절하는 데 기여하고자 그 공간적 패턴을 분석하였다. 본 연구는 Getis-Ord의 Gi* 와 평균 중심점을 이용하여 2001년부터 2014년 사이 말라리아 발생의 공간적 분포를 탐색적으로 분석하여, 그 시공간적 변화를 파악하였다. 또한 일반화 포아송 회귀모형을 이용하여 2001년, 2014년 말라리아에 영향을 미치는 인문·환경 요인을 비교·분석하고, 군사분계선으로부터의 거리 변수를 이용하여 그 공간적 자기상관을 설명하고자 하였다. 그 결과, 소 두수, 표준공시지가, 성비, 아파트 비율, 군사분계선 거리, 기후 요소, 논 비율이 말라리아 발생에 영향을 미치는 유의한 변수로 확인되었다.

Riptortus pedestris is a polyphagous species and an important pest of soybean. We investigated the phenology of R. pedestris at 14 locations - Jeonju, Naju, Goheung, Namhae, Milyang, Gunwi, Andong and 7 areas of Jeju. Commercial pheromone monitoring traps were used. The traps were baited with lures containing a 1:5:1 ratio of myristyl isobutyrate, (E)-2-hexenyl (E)-2 hexenoate and (E)-2-hexenyl (Z)-3-hexenoate. There was difference the first adult catch and nymph emergence among locations. To develop spring emergence distribution pattern, we used the trap data from each location. Spring emergence by sampling date was cumulated and analyzed relative to the Julian date using Weibull distribution function.

We analyzed the variation in occurrence time and intensity of attacks by the rice stem borer (Chilo suppressalis Walker) on several crops. We found similar occurrence patterns of C. suppressalis in different crops. Overwintering larvae showed no difference in pupal period and weight compared to the non-overwintering ones. However, the larval period was reduced to 19.1 days in Miscanthus field, in contrast to that in the paddy fields. Efficiency of the emergence trap was confirmed to be greater than that of the delta trap for capturing C. suppressalis adults. It is necessary to adjust the control period because of the advancement in occurring time of C. suppressalis in recent years. The larvae of C. suppressalis experience favorable environmental conditions for overwintering in Miscanthus fields. The results of this study provide important inputs for the development of environment-friendly control methods.

The adult activity of M. alternatus caught in a pheromone trap showed a bimodal form consisted of the first peak in mid to late June and the second peak in mid to late September in Jeju area, Korea. The two peaks were separated apparently between mid and late August, showing a valley. The pine trees without oleoresin flow were abundant during early July to early August, and declined thereafter, which did not match with the valley of adult activity curve. Thus, the hypothesis that dying pine trees attract much strongly M. alternatus adults than that of pheromone lures may not fully explain the bimodal pattern, because the first adult activity peak occurred during the peak period of dying pine trees and it declined with decreasing dying pine trees. The accumulated degree-days showed that the emergence of the 2nd generation adults could occur before the second peak when the diapause ecology of M. alternatus was not considered. The voltinism of M. alternatus can affect basically the control strategy of this pest. Consequently, further studies are required to find out clearly the voltinism of M. alternatus in Korea.

Spodoptera litura (Fabricius, 1775) is considered as a serious agricultural pest, which damaged to the many crops including sweet potato (Ipomoea batatas). We investigated weekly occurrence of the Spodoptera litura (Fabricius) using pheromone trap at six study fields of three regions, Jeonnam, Korea. A total of 41,895 individuals were collected from 18th July to 23th September in 2013; Yeongamgun was collected the highest individuals (17,519), and following the Muan-gun (12,709) and Haenamgun (11,667). The seasonal occurrence of S. Litura was increased from July to September, and peak occurrence timing was in late-September. There were a positive correlation between field area and number of individuals (P=0.02, r=0.89), and a negative correlation between field area and density (p=0.02, r=-0.89). As a PCA (principal component analysis) result, adults occurrence pattern were closely related to the temperature and relative humidity during larva stage.

As an indicator of skin health, acidified skin surface pH ranging from 5 to 7 is crucial for maintaining skin barrier. In this study, we evaluated the relationship between skin pH and dietary pattern (DP) as well as nutrient or food intake in 48 healthy middle aged adults. Skin pH was measured in the skin surface of the inner arm, and blood lipid profile was analyzed. Dietary intake data were obtained using 1 day 24 hour recall method, and DP was extracted using factor analysis. Results revealed that skin pH ranged from 5.15 to 6.88 in all subjects. There was no significant difference in skin pH between males and females. When subjects were grouped by tertile of skin pH, the food intake of fruit, and the nutrient intake of omega 6 fatty acid, potassium, vitamin A, vitamin C, β-carotene, and riboflavin in the first tertile group with skin pH ranging from 5.15 to 5.68 were significantly higher than in the third tertile group with skin pH ranging from 6.26 to 6.88. There was no difference in blood lipid profile between the first and the third tertile group. Among 5 DP extracted by factor analysis, DP5 characterized by a high intake of nuts and fruits as well as a low intake of beverages and alcohol was inversely correlated with skin pH after adjusting for gender and age. DP5 was positively correlated with nutrient intake of carbohydrate, fiber, potassium, iron, vitamin A, vitamin C, β-carotene, thiamine, and riboflavin but negatively correlated with sodium after adjusting for gender, age, smoking, and energy intake. Therefore, acidified skin pH could be maintained by these DP and nutrients.

The study concentrates on the status of ecological environment development in Shenyang, uses the remote sensing images and social statistics in different periods as the data base, establishes a dynamic monitoring system and model of urbanization and ecological security. Through the identification and quantitative analysis of ecological security dynamic monitoring information, with the support of spatial information technology, we have formed a key technology system which combines ecological security warning with ecological security structure optimization. Moreover, the spatial database of ecological key elements in Shenyang city was constructed. The vertical pattern, horizontal pattern and ecological function of urban ecological system is organically combined, which provides the basic data for the layout and regulation of green space and water system. With these efforts, a key technology system is formed, including the warning of urban ecological security- the space regulation of economic development- the optimization of urban ecological security pattern - the regulation of urban elements' spatial layout. The research results provide scientific and accurate data base for ecological planning and regulation in Shenyang City , carry out effective ecological security monitoring and management for the development of Shenyang, protect the ecological integrity of the system as well as the healthy development of the economy in Shenyang City during the urbanization process .

The purpose of this study is to inquire original design and character of dancheong in Yeongnamnu Pavilion which features unusual portraits of twisting dragons and four heavenly creatures. Its artistic value and originality can be found in the portraits of four heavenly creatures which are painted on the interior seonjayeon(fan shape rafter) and in the unique design of crossbeam meoricho(flower decorations on each side of pillars). Yeongnamnu’s crossbeam meoricho is janggu-meoricho type(meoricho with hourglass figure) with full-shape lotus and half-shape flower decorations. And it can be said that, dragon portrait painted on the border of lotus and flower decorations in green and yellow is a very unique style of dancheong, for the reason that it has scarcely been used before and ever since. The portrait of four heavenly creatures painted on each corner of seonjayeon is also found to be unique in design, for the reason that the design has rarely been used throughout history, with only two exceptions in mural tombs of Goguryeo and folding screen in Injeongjeon Hall of Changdeokgung Palace . With its unique and authentic feature along with its rarity in number, the portrait of four heavenly creatures painted on Yeongnamnu can be considered as quite symbolic and important.

OBJECTIVES: The objective of this study is to detect road cavities using multi-channel 3D ground penetrating radar (GPR) tests owned by the Seoul Metropolitan Government. METHODS: Ground-penetrating radar tests were conducted on 204 road-cavity test sections, and the GPR signal patterns were analyzed to classify signal shape, amplitude, and phase change. RESULTS : The shapes of the GPR signals of road-cavity sections were circular or ellipsoidal in the plane image of the 3D GPR results. However, in the longitudinal or transverse direction, the signals showed mostly unsymmetrical (or symmetrical in some cases) parabolic shapes. The amplitude of the GPR signals reflected from road cavities was stronger than that from other media. No particular pattern of the amplitude was found because of nonuniform medium and utilities nearby. In many cases where road cavities extended to the bottom of the asphalt concrete layer, the signal phase was reversed. However, no reversed signal was found in subbase, subgrade, or deeper locations. CONCLUSIONS: For detecting road cavities, the results of the GPR signal-pattern analysis can be applied. In general, GPR signals on road cavity-sections had unsymmetrical hyperbolic shape, relatively stronger amplitude, and reversed phase. Owing to the uncertainties of underground materials, utilities, and road cavities, GPR signal interpretation was difficult. To perform quantitative analysis for road cavity detection, additional GPR tests and signal pattern analysis need to be conducted.

Graphene has shown exceptional properties for high performance devices due to its high carrier mobility. Of particular interest is the potential use of graphene nanoribbons as field-effect transistors. Herein, we introduce a facile approach to the fabrication of graphene nanoribbon (GNR) arrays with ~200 nm width using nanoimprint lithography (NIL), which is a simple and robust method for patterning with high fidelity over a large area. To realize a 2D material-based device, we integrated the graphene nanoribbon arrays in field effect transistors (GNR-FETs) using conventional lithography and metallization on highly-doped Si/SiO2 substrate. Consequently, we observed an enhancement of the performance of the GNRtransistors compared to that of the micro-ribbon graphene transistors. Besides this, using a transfer printing process on a flexible polymeric substrate, we demonstrated graphene-silicon junction structures that use CVD grown graphene as flexible electrodes for Si based transistors.

본 연구는 주남저수지를 대상으로 빠르게 확산되고 있는 연꽃군락의 분포와 고니류의 활동범위를 파악하여 연꽃군락 의 확산과 고니류 월동지 이용패턴의 관계를 확인하고자 수행하였다. 연꽃의 확산 시기와 더불어 전반적인 낙동강 하구 일원의 고니류 개체수 변화추이를 살펴본 결과 연꽃의 확산과 주남저수지 월동개체수의 관련성은 뚜렷하지 않았 다. 주남저수지 내 연꽃군락의 분포는 2013년에 비해 2014년도에 수면 대비 비율이 13.2%에서 19%로 확산이 지속되었 으며, 고니류 개체는 연꽃군락 내부와 경계부 100m이내와 외부로 구분하였을 때 1차(2013년 12월 17일) 조사는 내부가 외부보다 약 3.1배 높은 밀도로 관찰되었으며, 3차(2014년 1월 29일)는 약 5.5배, 4차(2014년 12월 3일)에는 약 7.5배로 모두 연꽃군락 내부 관찰밀도가 매우 높았다. 2014년 1월 12일 2차조사는 탐방객이 집중하는 휴일 조사로 이러한 경향은 나타나지 않았다. 이는 고니류의 먹이활동 가능지역과 연꽃군락의 적정 생육공간이 일반적으로 수심 1m 남짓한 유사지역이라는 데에서 기인할 수도 있다. 그러나 연꽃군락의 증가가 고니류의 개체수 감소로 이어지지 않고 오히려 연꽃군락 중가와 함께 고니류가 지속적으로 증가하는 것으로 보았을 때 최소한 상충관계로 작용하지는 않음을 확인할 수 있었다.

Clematis patens는 미나리아재비과 으아리속에 속하는 다년 생 숙근초로 꽃잎화한 꽃받침을 가지고 있는 점이 특징이며 흰색, 분홍색, 자주색, 보라색, 등 다양한 화색이 존재한다. 본 연구에서는 C. patens ‘The President’에서 flavonoid 생합성 경로에 관여하는 유전자 중 delphinidin 계열 anthocyanin 색 소 합성을 유도하는 F3'5'H(ClF3'5'H)를 분리 동정하였으며, 화색 및 발달 단계별 ClF3'5'H 유전자의 발현 패턴을 분석하 였다. 또한 클레마티스 화색별 anthocyanin의 함량을 알아보 았다. 그 결과, 기존 보고된 Aconitum carmichaelii의 F3'5'H 유전자의 아미노산 서열과 79% 일치하여 높은 상동성을 갖는 것을 확인하였으며, ClF3'5'H 유전자의 발현 패턴은 흰색을 띠 는 클레마티스에서는 ClF3'5'H 유전자의 발현량이 많지는 않 았으나 다른 발달 단계에 비해 완전 개화한 단계에서 발현이 강했다. 분홍색을 띠는 클레마티스에서는 모든 발달 단계에서 ClF3'5'H 유전자의 발현이 검출되지 않았으며, 자주색과 보라 색을 띠는 클레마티스에서는 개화 직전 및 완전 개화 단계에 서 발현하였다. 이 결과로부터 클레마티스의 다양한 화색과 클 레마티스의 F3'5'H 유전자 발현과의 상관관계가 시사되었다.