본 연구는 아메리카동애등에(Hermetia illucens)의 대량 사육을 위하여 남은음식물 사료의 최적 수분 함량과 적정 사육 밀도를 조사하였다. 실 험에서는 500 g의 습식 사료의 수분 함량을 각각 30%, 50%, 70%, 90%로 조정한 후, 3,500마리의 3령 유충을 7일 동안 사육하였다. 그 결과, 90% 수분 함량에서 평균 유충 무게는 7.19 g으로 측정되었고, 70% 수분 함량에서 가장 높은 무게인 10.54 g이 나타났다. 반면, 50%와 30% 수분 함량에 서는 유충 생육이 저조한 결과가 나타났다. 적절 사육 밀도를 조사를 위해 100,000, 150,000, 200,000, 250,000 마리의 3령 유충을 70% 수분함량 의 100 kg 습식 사료에 7일 동안 처리하였다. 그 결과, 250,000 마리에서 총 유충 생산량이 22.79 kg으로 가장 높게 나타났으나, 150,000, 200,000, 250,000 마리 밀도 간에는 유의미한 차이가 없었다. 100마리당 평균 무게는 100,000 마리에서 16.90 g, 150,000 마리에서 15.20 g, 200,000 마리 에서 11.30 g, 250,000 마리에서 9.50 g으로 확인되었으며, 100,000 마리와 150,000 마리 밀도 간에는 유의미한 차이가 없었다. 결론적으로, 남은 음식물 사료인 습식사료 100 kg를 급여할 경우 수분 함량을 70%로 유지하고 사육 밀도를 15만 마리로 설정하는 것이 가장 효과적임을 확인하였다.

To determine the differences in food quality between fish fed a low fish meal diet containing black soldier fly (Hermetia illucens) instead of fish meal and those fed a general fish meal diet, we analyzed the approximate components, mineral content, amino acid composition, fatty acid composition, color, and texture of Flounder (Paralichthys olivaceus). The analysis of approximate components showed no difference between the two samples, except for moisture content. Mineral contents were measured in the order of K, P, Na, and Mg in both samples, with no difference except for phosphorus (P). A total of 16 amino acids were detected in both samples, with no significant differences in their composition. Additionally, 17 types of free amino acids were identified, with no significant differences between the two samples. The fatty acid composition consisted of 13 fatty acids, with palmitic acid, DHA, and oleic acid being the most prominent, although slight differences in content were observed. The color and texture also showed no differences between the two samples. Overall, there were no significant differences in chemical components or physical characteristics, so it was judged to be insignificant in terms of food science.

Supplementing feed components is becoming increasingly difficult for various reasons, including increased shipping costs, decreased capture efficiency, and global warming. For this reason, much research has been conducted globally to find alternative protein sources. The black soldier fly (BSF; Hermetia illucens) is an important feed item for nutritional accumulation and has environmental consequences. Few studies have been conducted to determine the nutritional level of the substrate, but most of these studies have involved modifying the substrate material. The aim of this study was to determine the ideal crude protein(CP) content of a substrate for BSF rearing. The experimental treatment was separated into three CP levels (low, 13.5%; medium, 20%; high, 26.5%) in the substrate. The results showed that the BSF weight (6.46%) and protein conversion ratio (1.13%) increased as the substrate CP level increased (p<0.05). In addition, protein (14.38%) and lipid (13.12%) yields were significantly increased (p<0.05). The BSF CP level increased as the substrate CP level increased (p<0.05). CP levels in the substrate upregulated the levels of certain fatty acids, essential amino acids, and non-essential amino acids (p<0.05). In conclusion, the potential values of BSF as feed were improved as the CP level of the substrate increased; However, no difference was found between medium and high CP levels in the rearing substrate. These results suggested that the substrate CP level should be at the medium level for optimal product quality. In conclusion, BSF quality is influenced by substrate nutrient levels, necessitating the development of specialized substrates for effective rearing.

For sustainable livestock industry, black soldier fly as eco-friendly animal protein source is spotlighted for swine and poultry. However, most of black soldier fly is reared in dirty condition like food waste. In this study, we investigated the effects of fiber level on the growth performance and nutrient composition of black soldier fly reared in specific substrate. Treatments were divided in two as Low (fiber level: 7.1%), and High (fiber level: 14.2%) with a total of 50,000 eggs each. The rearing box had a dimension with 1064 × 507 × 320 cm (length × width × height). Black soldier fly survival rate (72.24 vs. 76.48) and substrate consumption (77.43 vs. 80.37) were greater in High than Low. However, bioconversion (10.59 vs. 9.80) and protein conversion (8.97 vs. 8.00) were increased in Low than High. Gross energy was higher in High compared with Low (4,133 vs. 4,208). Some of essential amino acid (Isoleucine, leucine, phenylalanine, threonine, tryptophan) were significantly higher in High compared with Low, and non-essential amino acid (alanine, aspartate, glycine, proline, serine, tyrosine) were higher in High compared with Low. Acids such as C12:0, C14:0, C15:0, and C20:1 were higher in High compared with Low, while C18:4 was higher in Low compared with High. As a result, black soldier fly reared in high fiber substrate might be a preferred option for rearing and producing a reliable animal protein source for monogastric animal.

Black soldier fly (BSF, Hermetia illucens) has been noted as an excellent feed ingredient. However, there is limited information on rearing and processing technology. Thus, this study was conducted to evaluate the substrates for rearing BSF and the optimal processing method for BSF performance. Study separated as 2 experiment, BSF rearing and drying method(Exp 1.) and EP-processing method(exp 2.). During the study, 30 clutches were reared, with 10 per substrate. Three substrates, namely food waste (FW), tofu by-product (TF), and vegetable waste with two drying methods, namely hot air dry (AD) and microwave dry (MW) at expanding (EP) ratios of 5:5 and 7:3, were examined by evaluating their rearing performance, nutrient contents, in vitro digestibility and lipid oxidation stability during storage (0, 14 and 28 days). In experiment 1, the rearing substrates and drying methods were evaluated. Compared with that of conventional methods (FW, AD), the TF substrates and MW method showed higher dry matter contents (3.43%) and in vitro digestibility (1.62%) but lower ether extract contents(3.53%; p<0.05). However, the malondialdehyde (MDA) concentration under MW treatments decreased during storage (5.77%, 4.69% and 3.24%; p<0.05). In experiment 2, compared with that of the 7:3 EP-BSF ratio, the 5:5 EP-BSF ratio showed higher in vitro digestibility (2.70%) and lower MDA concentration during storage (19.19%, 7.96% and 6.42%; p<0.05). In conclusion, the optimal conditions for BSF rearing and ensuring product quality were TF substrates, MW methods and a 5:5 corn:BSF ratio. Therefore, the optimal conditions for producing EP-BSF can present an excellent feed ingredient alternative for swine feed.

Black Soldier Fly Larvae (Hermetia illucens, BSFL) is an environmental purification insect utilized as an animal feed source due to its high protein content. BSFL need to be dried to preserve quality, and the optimal drying method should be cost-effective. In this study, various drying methods (hot air (HD), freeze (FD), vacuum (VD), and combined-drying (CD)) were applied to BSFL, and then BSFL powders were characterized. Crude protein content was highest in HD and lowest in CD, and there was no significant difference in crude fat content (p>0.05). In the measurement of the total amino acid content of BSFL, glutamic acid and aspartic acid were the highest regardless of the drying method. The total amino acid content was the highest in HD and the lowest in CD. Total polyphenol content was higher in VD and FD than in HD and CD. Also, hydrogen peroxide and DPPH radical scavenging showed similar results. In the antioxidant measurement results, VD and FD showed higher antioxidant capacity, but considering cost-effectiveness, HD was the most available method for drying BSFL.

This study investigated the quality characteristics of emulsion-type chicken breast sausages containing black soldier fly larvae (BSFL) powder. The chicken breast and BSFL powder were added at various mixing ratios (C:chicken breast 56% and BSFL powder 0%, 1%:chicken breast 55% and BSFL powder 1%, 2%:chicken breast 54% and BSFL powder 2%, 3%:chicken breast 53% and BSFL powder 3%). The pH, moisture content, and water holding capacity of the sausages increased with the powder amount. In the instrumental color evaluation, the L* and b* values were significantly different among the samples (p<0.05). However, there was no significant difference in a* value (P>0.05) with the amount of powder added. Volatile basic nitrogen and acid value tended to increase with the amount of BSFL powder. The total phenolic content tended to increase, but the value of IC50 according to DPPH radical scavenging decreased as the amount of powder increased. This study concluded that BSFL powder can be used in meat products processing as a substitute for meat protein and may also improve antioxidant activity.

이 연구에서 채집된 아메리카동애등에의 성충은 원산지의 성충과 그 크기가 비슷했다. 체형에서 가장 큰 차이점을 보이는 부분은 더듬이 바깥쪽 (antenna 2) 길이이며, 그 다음으로는 머리넓이 (head width), 복부넓이 (abdomen width) 등의 순으로 나타났다. 이 연구결과는 유기물 분해와 분변토 생산의 우수개체를 선발하는데 기초자료가 될 것이다. 국내 51개 조사지역 중, 22개 지역에서 아메리카동애등에가 출현했으며 위경도 및 고도 등 지리적 특성보다는 유기물의 존재에 더 영향을 받는 것으로 생각된다. 이 곤충의 발생현황은 유기성 폐자원의 선순환 구조를 파악할 수 있는 지표로 사용될 수 있을 것이다.

외래생물인 아메리카동애등에는 뛰어난 유기물분해능으로 주목받아왔다. 이에 우리는 한국내 분포상과 유전적 다양성을 알아보기 위해 전국적으로 채집하여 미토콘드리아의 사이토크롬 C 산화효소 1 (CO1) 유전자를 이용해 집단유전학분석을 실시하였다. 그 결과 이 곤충은 한국에 성공적으로 정착한 것으로 보이며 10개의 미토콘드리아 유전자형이 있었고 개체군 간 분화가 뚜렷했다. 이 결과는 국내정착한 아메리카동애등에가 특정 유전자형의 모계만이 도입된 후 지역 간 분산이 제한적임을 나타내는 것으로, 이는 이들의 단거리 비행 경향에 기인한 것으로 추정된다.

아메리카동애등에(Hermetia illucens) 유충 및 번데기를 대량사육하여 가축사료로 활용하기위해 성충사육상과 산란처의 산란조건을 비교 조사하였다. 성충사육상은 재질에 관계없이 가로, 세로, 높이 2 m 이상의 사육상에서 주당 성충 20,000개체를 투입하여 2일간 채란한 난괴수가 평균 628.0 ~ 648.0개 이었고 1.8 ~ 1 m의 사육상에서는 86.7 ~ 418.7 난괴로 적었다. 산란처를 골판지로 사용하였을 때 41.0 ± 37.7난괴를 산란하였고 플로랄폼에는 326.6 ± 68.4난괴, 녹색테이프를 붙인 골판지는 135.2 ± 41.5난괴를 산란하여 녹색의 산란처를 선호하는 것으로 조사되었다. 골판지에 플로랄폼을 덧씌운 산란처에는 평균 57.7개로 플로랄폼의 64.7개와 유사하게 산란하였다. 산란처의 산란홀 직경은 4 ㎜에서 평균 48.2난괴를 산란하여 직경 6 ㎜의 29.0난괴보다 많았다.

아메리카동애등에(Hermetia illucens)를 대량사육하기위해 발육 기간 동안 음식물건조사료를 주성분으로한 먹이로 조성을 달리하여 사육하였다. 먹이조성비율은 산란된 알의 부화유도배지, 부화유충 1, 2단계 먹이로 서로 다르게 제작하였다. 부화유도배지는 육성돈사료와 물을 4 : 6의 비율로 혼합하고 상부에 밀기울을 3 ㎝ 두께로 덮었다. 부화유충 1단계먹이는 음식물건조사료(50%), 맥주박(40%), 밀기울(9%)에 당밀과 발효액을 각각 0.5%씩 혼합하였고 부화유충 2단계먹이는 음식물건 조사료(99%)에 당밀과 발효액을 0.5%씩 혼합하였다. 부화유충먹이의 함수율은 약 60%로 제작하였다. 부화유도배지에서 동애등에유충은 부화 후 3일간 사육한 후 유충 1단계 먹이로 7 ~ 8일간 사육하였고 2단계 먹이로 6 ~ 7일간 사육하였다. 사육한 종령 및 번데기는 수확하기 전 2일 동안 먹이를 공급하지 않았다. 동애등에유충과 번데기를 수확하기위해 진동채로 분변을 분리하여 수확하였다.