현재의 문화 교육이 지니는 근본적인 문제는 과거와 현재, 자국과 타국의 문화를 이분법적으로 바라 보고 개별적 정보 위주의 접근 방식을 벗어나지 못하고 있다는 점이다. 이 논문의 목표는 한국의 전통 문화에 대한 반성적 성찰을 통해 바람직한 문화 교육의 방향을 모색하려는 것이다. 이를 위해 인문학적 인 관점, 비교문화적인 관점, 통시적인 관점을 적용하여 문화와 예술을 아우르는 종합적인 안목을 확보 하고자 하였다. 그리고 상호 문화 역량과 문화 수용 능력을 제고하기 위하여 문화 현상 간의 비교를 시도하였다. 본론에서는 두 단계로 나누어 논의를 진행하였다. 첫째, 한국 영웅 서사의 연원과 현대적 활용 가능성을 모색함으로써 총괄적 논의를 위한 단서를 마련하였다. 전통 서사의 문화적 의미와 미학 적 원리에 대한 재인식을 통해 인류의 문화가 보편성의 차원에서 상호 소통할 수 있음을 확인하였다. 둘째, 현대 디지털콘텐츠에 활용된 문화 요소들을 중심으로 한국인의 생활과 예술 분야에 나타나는 문화적 정체성과 시대적 변용 양상을 고찰하였다. 성숙한 문화는 다양성의 가치를 인정하는 것에서 출발하며, 문화 요소 전반을 관통할 수 있는 감식 능력과 소통 능력이 요구된다는 점을 확인하였다. 바람직한 문화 교육은 창의적인 미래 문화를 이끌어 갈 수 있는 능력을 길러 주는 방향으로 이루어져야 할 것이다.

The IMO’S 72nd MEPC meeting proposed the goal of reducing greenhouse gas emissions by up to 50% by 2050. Thus, various eco-friendly fuels are proposed as alternatives, but there are also various issues that need to be tackled, such as storage stability and supply system issues in a special environment a ship has. Therefore, in this study, the possibility of reducing greenhouse gases was analyzed by applying MGO as an alternative to boilers operated with HFO, a Bunker-C series. As a result, the exhaust gas temperature decreased by about 11.54% from 316.9℃ to 280.3℃, and the amount of oxygen content increased by about 0.38% from 6.27% to 6.65%. It can be seen that carbon monoxide can be reduced by about 45.28% by simply converting fuel from 45.29 ppm to 24.78 ppm, and carbon dioxide, which is a typical greenhouse gas, can be reduced by about 0.49% from HFO by 11.08% to MGO by 10.59%. This means that some greenhouse gas reduction is possible only by shifting between ship fuels that satisfy ISO-8217, but since there are limitations to achieving strong carbon neutrality proposed by IMO, it will be necessary to actively utilize the use of various alternative fuels in the future.

This study aimed to evaluate the nutritional composition of various edible and medicinal mushrooms and assess their potential as alternative food sources in response to increased meat consumption, which poses environmental and health risks. The crude protein, crude fat, carbohydrate, dietary fiber, and vitamin C contents of 17 edible and medicinal mushroom species, including Flammulina velutipes, Pleurotus ostreatus, Pleurotus eryngii, Agaricus bisporus, Lentinula edodes, and Wolfiporia extensa, were systematically analyzed. Pleurotus ostreatus‘Suhan 2’ and P. eryngii‘No. 2’ showed high crude protein and carbohydrate contents, suggesting excellent potential as alternative protein and energy sources. Flammulina velutipesvarieties ‘TO22’ and ‘Aram’ exhibited relatively high crude fat contents, whereas P. eryngii‘No. 2’ and P. ostreatus‘Suhan 2’ had high vitamin C levels, suggesting a beneficial role in immune enhancement. In contrast, the medicinal mushroom W. extensa had relatively low protein and dietary fiber contents, limiting its potential as an alternative protein source compared to the other edible mushrooms. Nonetheless, it may provide other health benefits. The present study provides foundational data for the utilization of mushrooms as sustainable alternative food resources, supporting efforts to mitigate environmental impacts of meat production and improve dietary health.

The rapid increase in global meat consumption, driven by population growth and economic development, has intensified concerns about the sustainability of protein supply. Moreover, conventional livestock production significantly affects the environment and human health, further underscoring the need for alternative protein sources. Alternative proteins, including those derived from plants, mycelium, and insects, as well as cultured meat, offer sustainable and nutritionally viable substitutes for traditional animal-based proteins. Among these, mushroom-based alternative proteins demonstrate exceptional environmental, nutritional, and functional advantages. In particular, mushroom-derived proteins can closely replicate meatlike tastes and textures, positioning them as promising candidates in the rapidly growing alternative protein market. In this review, we provide a comprehensive analysis of recent advancements in the production of mushroom-based alternative protein, focusing specifically on mycelium cultivation techniques, edible mushroom applications, and production process innovations. Furthermore, we evaluate the nutritional composition, bioactive compounds, and associated health benefits, along with economic feasibility and consumer acceptance, all supported by empirical evidence. We extensively discuss technological innovations aimed at optimizing texture and reducing production costs. Finally, we propose concrete policy recommendations for fostering sustainable growth in the alternative protein industry, emphasizing regulatory frameworks, research investments, and consumer education strategies.

Fetal Bovine Serum (FBS) plays a crucial role in animal cell culture; however, the increasing number of bovine fetuses used and sacrificed solely for FBS collection has raised ethical concerns globally. The welfare of fetuses during FBS blood collection has become a key focus of debate among animal welfare and ethics organizations worldwide. Previous studies indicate that heat-inactivated coelomic fluid (HI-CF) from the earthworm Perionyx excavatus may serve as a viable FBS alternative in adherent cell cultures. This study evaluates the potential of HI-CF as an FBS substitute during the in vitro maturation (IVM) stage of bovine embryo culture, with a focus on improving developmental rate through antioxidation effects. In this study, 2% HI-CF was incorporated into IVM media, assessing its impact on cell growth, differentiation, and the expression of genes related to antioxidation. The group of 2% of HI-CF exhibited a trend toward increased cleavage and blastocyst development rates compared to the control group. Although antioxidant genes such as NRF2 and GSR showed no statistically significant differences between the control and treatment groups, a trend toward increased expression was observed. Conversely, GPX1 displayed a trend of decreased expression. Notably, IGF1 and NQO1 were significant upregulated (p < 0.05) in the 2% HI-CF group. Additionally, oocytes stained with H2DCFDA showed a significantly reduced ROS levels (p < 0.05) in the 2% HI-CF group compared with controls. These findings suggest that HI-CF's antioxidative effects support enhanced cell growth and blastocyst development rate, surpassing those observed with FBS. Consequently, HI-CF shows promise as an effective alternative to FBS in vitro maturation of bovine oocytes.

This study was conducted to confirm whether domestic fruit tree pruning residues can replace sawdust, which is the main ingredient of mushroom medium. The five types of fruit tree pruning residues collected were from apples, pears, peaches, grapes, and citrus. The basic components of these residues were analyzed. The pH ranged from 5.2 to 7.0, the Total carbon(T-C) ranged from 46.0% to 47.2%, the Total nitrogen(T-N) ranged from 0.5% to 0.9%, and the moisture content ranged from 12.4% to 14.2%, which was identified as an appropriate range for growing mushrooms. In order to confirm the possibility of mycelial growth of oyster mushroom "Suhan", column tests were conducted based on the conventional medium(poplar sawdust 49.5: cotton seed hull 27.3: beet pulp 12.7: cotton seed meal 10.5, v/v). As a result of incubation at 25°C for 28 days, grape branches showed the fastest growth at 143 mm compared to the control, which showed 135 mm. The yield per bottle was comparable, with grape branches(134g/bottle) and control(139g/bottle). Additionally, the quality of the fruiting bodies was comparable across all Processed lots. It means grape branches can be used as alternative sawdust materal. In the future, it is expected that using by-products as substitutes for carbon sources and main ingredients will help reduce farm production costs and protect the environment.

This study examined domestic consumption trends in the development of mushroom-based alternative food products. It found that 85.5% of consumers were aware of alternative foods, with a higher recognition rate among younger age groups and variations based on household composition and monthly income. Additionally, 70% of consumers familiar with alternative foods had tried them. Among those who had not, taste (16%) and purchasing challenges (15%) were the primary barriers. Minced meat was the most commonly consumed alternative (25%), while dumplings (7%) and burgers (1%) were less popular. Notably, dumplings and burgers showed the highest potential for development using mushroom-based ingredients. These findings provide valuable foundational data for advancing mushroom-based alternative food products.

Recently, active research in Korea and worldwide has begun to focus on gene function and cultivar development using gene editing tools. This research, in addition to studies on edible mushroom, aims to enhance the physical and biochemical characteristics of mushrooms for applications in materials and substance production. For these studies, efficient isolation of protoplasts from the target mushroom is critical. However, several commercial cell wall-lysing enzyme cocktails, including Novozyme234, Glucanex, and Lysing enzymes, have recently been discontinued. In this study, we aimed to identify alternative enzyme systems to replace the discontinued cell wall-lysing enzymes for stable isolation of protoplasts from Ganoderma lucidum. To select an optimal osmotic buffer, enzyme function in 0.6 and 1.2 M Sorbitol, Sucrose, Mannitol, and KCl was assessed. The effect of reaction time was also evaluated. Protoplast isolation efficiency of each alternative enzyme was tested using lysing enzymes from Trichoderma harzianum, Chimax-N, and Yatalase, either individually or in combination. This matrix of studies identified enzymes and optimal conditions that could replace the discontinued lysing enzymes.

The alternative food market has attracted much attention due to concerns about climate change, increasing consumer awareness of value consumption, and the development of the Food-Tech industry. This study aimed to analyze the factors that drive consumers to purchase alternative food products and identify the mechanisms that can induce consumers to continue buying. We surveyed 1,200 consumers and estimated data using a logit model. The results showed that the presence of vegetarians in the household, environmental concerns, a vegetable-oriented diet, and a mixed diet of meat and vegetables were positively associated with purchasing plant-based alternatives. Of particular interest was the non-linear relationship between respondents’ age and their purchasing experience with plant-based options, with the likelihood of purchasing alternatives increasing with age from the mid-50s onward. These findings suggest that in addition to the growing number of consumers who share the environmental value, which leads to increased interest in and purchase of plant-based alternatives, alternative foods are also health-oriented and meet the needs of older consumers, who are becoming an increasingly important segment of the super-aged society, suggesting the potential for continued growth in the alternative food market.

이 연구는 최근 민주주의 위기 원인을 자유주의가 신자유주의로 극단 화되고 민주주의가 포퓰리즘으로 급진화함에 따른 자유민주주의의 해체 상황으로 파악하며, 이로 인해 대안적 정치원리로 공화주의가 부상하고 있음을 주장한다. 흔히 공화주의는 민주주의와 동일시되며, 개인을 강조 하는 자유주의에 대한 대안으로 이해되고는 한다. 그러나 공화주의에 대 한 20세기 후반부터의 연구는 자유주의의 부작용은 물론 민주주의의 한 계까지 극복할 수 있는 공화주의 사상의 가능성에 주목해 왔다. 특히 냉 전 해체 이후 자유민주주의에 내재한 자유주의와 민주주의 사이의 갈등 이 심화됨에 따라 공화주의는 본격적인 대안적 정치사상으로 주목받기 시작했다. 이처럼 본 연구는 기존의 공화주의 관련 연구들이 공화주의 자체의 내재적 가치에 집중하는 것과는 달리, 최근 공화주의의 부상을 자유주의의 극단화와 민주주의의 급진화라는 역사적 구조 속에서 파악하 려 한다. 이와 함께 서구 고대로부터 기원한 공화주의를 현재화하기 위 한 혁신의 과제들을 검토하고 대안적 사상으로서 공화주의의 함의를 고 찰한다.

Background: The small intestine plays a crucial role in animals in maintaining homeostasis as well as a series of physiological events such as nutrient uptake and immune function to improve productivity. Research on intestinal organoids has recently garnered interest, aiming to study various functions of the intestinal epithelium as a potential alternative to an in vivo system. These technologies have created new possibilities and opportunities for substituting animals for testing with an in vitro model. Methods: Here, we report the establishment and characterisation of intestinal organoids derived from jejunum tissues of adult pigs. Intestinal crypts, including intestinal stem cells from the jejunum tissue of adult pigs (10 months old), were sequentially isolated and cultivated over several passages without losing their proliferation and differentiation using the scaffold-based and three-dimensional method, which indicated the recapitulating capacity. Results: Porcine jejunum-derived intestinal organoids showed the specific expression of several genes related to intestinal stem cells and the epithelium. Furthermore, they showed high permeability when exposed to FITC-dextran 4 kDa, representing a barrier function similar to that of in vivo tissues. Collectively, these results demonstrate the efficient cultivation and characteristics of porcine jejunum-derived intestinal organoids. Conclusions: In this study, using a 3D culture system, we successfully established porcine jejunum-derived intestinal organoids. They show potential for various applications, such as for nutrient absorption as an in vitro model of the intestinal epithelium fused with organ-on-a-chip technology to improve productivity in animal biotechnology in future studies.

MEPC 80차 회의에서 IMO는 더욱 강화된 온실가스 감축 전략을 제시하였다. 기존의 72차 회의에서 제시하였던 초기전략보다 구체적이고 강화된 감축 전략이다. IMO는 2050년 무렵까지 국제 해운으로부터 온실가스 배출을 ‘Net Zero’에 도달하도록 전략을 세웠다. 이 논문에서는 대표적인 친환경 연료로 구분되는 LNG, 수소, 메탄올, 암모니아의 위험도 평가를 진행하였다. 전문가들의 설문조사를 통한 결과를 퍼지 기법을 적용하여 주관적인 모호성을 해결하였다. 또한 TOPSIS 기법을 통해서 퍼지의 긍정적인 해와 부정적인 해를 도출하였 다. 이를 통해서 Vertex 방법을 이용하여 대체 연료의 근접계수 값을 최종적으로 구하여 결정하였다. 그 결과, 메탄올, LNG, 수소, 암모니 아 순으로 선호하였다. 이 연구는 제안된 접근 방식이 대체 연료를 결정을 위한 집단 의사결정 방법으로 이용할 수 있음을 보여준다.