Pleurotus species are the most consumed and cultivated mushrooms in Korea. Although oyster mushrooms (P. ostreatus) can be cultivated automatically, their storability is slightly lower than that of king oyster mushrooms (P. eryngii) and winter mushrooms (Flammulina velutipes); therefore, the export proportion of oyster mushrooms is very low. Since Korean mushrooms are highly preferred across Southeast Asian, the export of oyster mushrooms in the form of complete substrates is expected to be more promising than that of fresh mushroom. Here, 1 and 2.5 kg complete substrates of P. ostreatus ‘Soltari’ and P. sajor-caju ‘Sambok’ were prepared and stored at different temperature from 0 to 15°C for 10 days. Thereafter, the formation of fruiting bodies was induced. Since the 2.5 kg complete substrates required 70 days of incubation, their mycelia were at an advanced age and their fruiting bodies did not grown normally. When 70%-incubated complete substrates were stored at 5–10°C, the growth was faster and more uniform and stable fruiting bodies were formed. Export test of complete substrates to Vietnam using distribution containers set at 0°C and 15°C revealed that the growth period was shortened by 1–2 days when the distribution containers were set at 15°C and the yield of ‘Soltari’ increased by approximately 10%. In addition, even though the yield of ‘Sambok’ was similar between treatments at 0°C and 15°C, the quality of fruiting bodies from 15°C-distributed complete substrates was much better than that of those from 0°C-distributed substrates.

This study was carried out to develop techniques for thelog cultivation of Pleurotus pulmonarius. Soil landfill cultivation of the in plastic container boxes containing yield per log thanbut there was no difference in the yield from both spawn Hyangsan” variety. In the caseof soil landfill cultivation in a shaded vinyl house, an Ailanthus tree gave a higher yield than that usingpoplar tree, and the yield of the Hosan” variety washigher than that of the Hyangsan” variety. With regard to proper tree species selection, willow and cherry trees were good for both the Hosan” and “Hyangsan” variet.

Oyster mushrooms including of P. ostreatus, P. eryngii, P. pulmonarius and P. cornucopiae are one of the famous mushrooms for foods in Korea. RAPD were carried out using 14 of oligoprimers to analyze the phylogenetic relationship among 57 strains of 32 Pleurotus species. Most of species formed the minimum clade with strains within species and was divided respectively species. Therefore clade was separated well in accordance species. Pleurotus species formed again clade to be added in close related to other species, and were discriminated by sixteen clades with each representative species including high similarity groups. Sixteen clades were composed representative species according to each clade. There were clade I of P. pulmonarius(P. sajor-caju, P. opuntiae, P. sapidus), clade II of P. eryngii(P. fuscus var. ferulae, P. fossulatus), clade III of P. ostreatus(P. ostreatus var. columbinus, P. spodoleucus, P. floridanus), clade IV of P. florida, clade V of P. djamor(P. flabellatus, P. incarnates, P. salmoneo-stramineus), clade VII of P. populinus(P. subareolatus), clade VIII of P. cystidiosus(P. cystidiosus var. formosensis), clade X of P. dryinus(P. dryinus var. pometi), clade XIV of P. cornucopiae(P. citrinopilieatus, P. euosmus), and clade XV of P. australis. These species were representative species each clades. Five species, P. ulmarius(clade VI), P. griseus(clade IX), P. calyptratus(clade XI), P. lampas(clade XII), P. smithii(clade XIII)and P. serotinus (clade XVI) were used each one strain in analysis, so they were clustered other groups.

Pleurotus has increased rapidly production and consumption because of highly nutritional value, natural healthy food and so on. The basic studies for Pleurotus need for development of mushroom industry. This study was to investigate the cultural characteristics among 15 strains of 6 species and to analyze their phylogenetic relationships. The cultural characteristics were investigated by mycelial growth activity at different media, temperature and pH. The optimum media for mycelial growth were YM and MCM in most species. The optimum temperature for mycelial growth were 25˚C and 30˚C. The optimum pH for mycelial growth were widly range from pH 5.1 to 7.4. Through the RFLP (restriction fragment length polymorphism) of IGS (intergenic spacer) I region in ribosomal DNA, it was analyzed phylogeny of interspecies and intraspecies. Each species was discriminated well as isolates within each species formed clade to be distinguished other species. P. florida was highly similar to P. floridanus, and P. flabellatus was P. cornucopiae. P. fuscus var. ferulae was highly similar to P. eryngii but discriminated different species in analysis of RFLP of IGS I region and showed different characteristics in mycelial culture. RFLP of IGS I region was useful of studying phylogenetic relationships of species and population.

천연 억새 1년, 2년, 3년생의 화학성분 분석결과 인산은증가하는 반면 칼슘과 칼리는 낮아지는 경향이었고 총 질소는 2년생 억새에서 증가하였고, 총 탄소는 3년생 억새에서 급격히 증가하는 경향으로 버섯배지의 이용성이 높게 평가되었다. 억새의 발효조건은 미강20%를 혼합할 경우 발효는 5일부터 5일간에 지속되고 이때 온도는 50-55oC로 유지되어 고온성 미생물 군이 형성됨을 알 수 있었고 처리효과 구명은 미강20% 첨가발효배지가 무처리억새배지보다 수량이 60% 향상되었다. 느타리봉지재배의억새배지는 포플러톱밥50%의 포플러톱밥 20%를 억새발효배지 20%로 대체한 혼합배지인 포플러톱밥30%, 억새발효배지20%, 비트펄프30%, 면실박20% 배지를 개발하였다.

In this study, the anti-oxidant, anti-tumorigenic, anti-hypertensive, anti-thrombic, anti-diabetic, and anti- inflammatory properties of 18 different species of genus Pleurotus were investigated. In addition, the amino acid, β-glucan, and polyphenol contents were also measured. The β-glucan and polyphenol contents were the highest in Pleurotus cornucopiae var. citrinopileatus (yellow Pleurotus) of all species. The yellow Pleurotus also exhibited the highest physiological activity as assessed by the DPPH IC, Cytotoxic activity, ACE activity and so on. To confirm the mechanism for the physiological activity of the yellow Pleurotus, we performed further examinations within ICR mice. The yellow Pleurotus reduced glucose, triglyceride and total cholesterol levels in the ICR mice blood for 4 weeks after feeding, and also significantly lowered both GOT and GPT levels. Taken together, our data indicates the yellow pleurotus is a promising functional food ingredient.