본 연구는 국내육성 수국 ‘Morning Star’와 ‘Pink Ari’에 있어서 삽목배지 및 삽목시기가 발근에 미치는 영향을 조사하 기 위해 수행하였다. 삽목배지 실험에서 코코피트, 펄라이트, 피트모스 단용과 코코피트+코코칩을 혼용 처리하였으며, 7월 15일에 ‘Morning Star’의 정아에서 삽수를 채취하여 삽목하 였다. 삽목시기 실험은 4월, 6월, 8월에 정아를 채취하여 삽 목하였다. 삽목배지로써 코코피트와 코코칩의 혼용 배지는 발 근에 가장 부정적인 영향을 미쳤다. 다른 배지들은 발근율과 뿌리길이에서 통계적인 차이가 없었으나, 펄라이트와 피트모 스보다 코코피트에서 뿌리수가 더 많았다. 따라서 국내육성 수국 ‘Morning Star’의 삽목은 코코피트를 배지로 사용하는 것이 발근에 가장 효과적인 것으로 나타났다. 삽목시기에 관 한 실험에서, ‘Morning Star’의 발근율은 삽목시기와 상관없 이 83~90%로 통계적인 유의성이 없었으나, 뿌리수와 생체 중은 4월과 8월보다 6월에 삽목했을 때, 가장 증가하였다. 그 리고, 신초의 길이, 엽수 등의 줄기의 생육도 6월에 삽목한 처 리에서 가장 좋았다. ‘Pink Ari’의 삽목시기 실험에서 발근율 은 4월 삽목보다 6월과 8월 삽목 처리에서 높았고, 뿌리수와 뿌리 생체중은 6월 삽목처리에서 각각 61.4개와 0.31g으로 가장 양호하였다. 또한 엽수와 신초의 길이 등 지상부 생육도 전반적으로 4월과 8월 삽목보다 6월 삽목처리에서 가장 좋았 다. 따라서, 국내육성 수국 ‘Morning Star’와 ‘Pink Ari’는 4 월과 8월보다 6월에 삽목하는 것이 발근과 지상부 생육에 효 과적인 것으로 나타났다.

본 연구는 장미 ‘Natal Briar’와 ‘Deep Purple’의 기내 대 량증식의 조건을 알아보고자 마디배양시에 신초의 형성과 생 육에 미치는 NAA와 cytokinin류의 처리 효과를 조사하였다. ‘Natal Briar’는 2.0과 5.0mg·L-1 BA의 고농도 처리는 신초 형성을 억제하였으며, 0.5mg·L-1 처리에서 신초수 3.7개, 복 엽수 7.1개, 엽수 24.6개로 다른 처리들보다 가장 많았다. Kinetin은 0.5mg·L-1의 저농도 처리보다 2.0과 5.0mg·L-1 의 고농도 처리에서 신초가 더 많이 형성되었다. TDZ는 BA 와 kinetin보다 전반적으로 신초의 수와 길이, 복엽수, 엽수 등에서 부정적으로 작용했으며, 특히 2.0과 5.0mg·L-1의 고 농도 처리에서 기형적인 잎들이 형성되었다. 그리고, NAA는 처리농도가 높아질수록 callus가 형성되었으며, 신초형성은 억제하였다. ‘Deep Purple’은 ‘Natal Briar’의 결과와 유사 하게 나타났는데, kinetin과 BA 처리가 TDZ 처리보다 신초 의 형성이 양호하였으며, 다른 처리들보다 0.5mg·L-1 BA 처 리에서 신초수, 복엽수와 엽수가 가장 많아 신초의 형성과 생 육에 가장 효과적이었다. 그리고, NAA 단용과 0.5mg·L-1 BA 와 혼용처리에서도 NAA의 농도가 높아질수록 대조구보다 신 초형성이 억제되었으며, 2.0과 5.0mg·L-1 NAA 처리에서 callus가 형성되었다. BA 0.5mg·L-1의 농도에서 형성된 ‘Natal Briar’와 ‘Deep Purple’의 신초는 1/2 MS 배지에서 발근되었다. 따라서 장미 ‘Natal Briar’와 ‘Deep Purple’는 마디배양시 0.5mg·L-1 BA가 첨가된 MS 배지에서 배양을 하 는 것이 신초 형성과 생육에 가장 효과적이었다.

본 연구는 대한민국 울릉도 특산식물인 추산쑥부쟁이(Aster × chusanensis Y.S.Lim, Hyun, Y.D.Kim & H.C.Shin)의 기내 대량증식체계 확립을 위해 시토키닌 계열 식물생장조절 제를 첨가하여 캘러스 및 신초 형성 조건을 구명하고자 수행 하였다. 실험결과, 추산쑥부쟁이의 줄기 절편체에서 캘러스 와 신초가 형성되었다. 캘러스 형성률은 TDZ 3.0mg·L-1처 리구에서 88.9%로 가장 높았고 BAP 3.0mg·L-1와 Zeatin 3.0mg·L-1 처리구는 66.7%, 2iP 3.0mg·L-1 처리구에서는 11.1%였다. 신초 형성률은 모든 처리구에서 77% 이상이고 특히 TDZ처리구와 Zeatin 처리구에서 100%였다. 그러나 TDZ처리구에서는 형성된 신초가 부풀어 오르거나, 잎에 과수 성이 나타나는 등 형태적 기형이 나타났다. Zeatin처리구에 서 발생한 신초는 정상적인 형태를 나타났다. 잎 절편체에서 는 캘러스, 신초가 발생하지 않았으며 절편체가 탈색하며 고 사하는 것을 확인하였다. 종합적으로 추산쑥부쟁이의 캘러 스 및 신초 형성을 위해서는 줄기 절편체를 배양 재료로 이 용하는 것이 효과적으로 확인되었다. 캘러스 유도에는 TDZ 3.0mg·L-1처리가 가장 효과적이었고, 추후 NAA와 같은 옥신 을 함께 첨가하여 증식 효과를 분석하는 연구가 필요할 것이 다. 또한, 신초 유도에도 TDZ 3.0mg·L-1 처리가 효과적이었 으나 장기간, 고농도로 사용할 경우 형태적인 기형이 발생할 수 있어 정상적인 식물생산에 부적합한 것으로 확인되었다. 따라서 형태적 기형이 발생하지 않은 아데닌 유도체형 시토키 닌인 Zeatin을 활용하는 것을 추천한다. 본 연구는 울릉도 특 산식물인 추산쑥부쟁이의 기내 대량증식과 생태계 유지에 필 요한 종 공급을 위한 대량증식 체계 구축을 위한 자료로 활용 할 수 있을 것이다.

Pinus densiflora is a fixed-growth coniferous species that elongates its shoot once a year and finishes growing in early summer. However, it may produce additional shoots in the same year in response to external stimuli, called abnormal shoot growth. This study investigated the effects of open-field summer warming and drought on the abnormal shoot growth of P. densiflora seedlings. In March 2022, two factorial combinations were constructed, including two temperature treatments (control and 4°C increase) and two precipitation treatments (control and drought), with five replicates for each combination. The temperature treatment was performed for 87 days from May 14 to August 8, 2022, and the precipitation treatment was performed for 33 days from May 14 to June 15, blocking 100% of the ambient rainfall. The abnormal shoot occurrence rate and leaf unfolding stages were measured in November, and the shoot and root collar diameter growth rates were calculated by comparing the seedling height and root collar diameter measured in August (after the cessation of treatment) and October (after the end of growing period) with the initial values (i.e., May 2022). The abnormal shoot occurrence rate significantly increased under the warming treatment, showing a 410.6% increase in the warming plots (38.4%) compared to the control plots (7.5%). There was no significant difference in the shoot and root collar diameter growth rate regarding warming and drought treatments. Abnormal shoots may have been affected by high temperatures by inducing early transition to the next ontogenetic stage.

기계적 자극은 식물체의 형태적 변화를 유도하는 것으로 알 려져 있다. 본 연구에서는 기계적 자극을 적용한 토마토의 육 묘기 동안의 생육 변화를 확인하기 위해 수행되었다. 브러싱 처리가 기계적 자극으로 이용되었으며, 아크릴이 부착된 이 송장치를 이용하여 2시간 간격으로 파종 후 10일 이후부터 처 리를 진행하였다. 브러싱 처리 동안 생육 변화를 확인하기 위 해 3일 간격으로 생육을 조사하였다. 초장과 엽면적은 브러싱 처리에서 대조구에 비해 감소하는 결과를 보였으며 지상부의 생체중과 건물중은 대조구와 유사한 값을 보였다. 총 근장과 지하부 표면적은 브러싱 처리에서 대조구에 비해 증가하였으며, 지하부 부피는 대조구와 브러싱 처리가 유사한 값을 보였 다. 결론적으로 토마토 공정묘에 브러싱 처리는 지상부의 생 육 감소를 유도하였으며 지하부의 형태적 발달을 증가시키는 것으로 판단된다.

Some plant pathogenic bacteria species are environmentally high-risk organisms that have a negative impact on agricultural production. Experiments with these pathogens in a biosafety laboratory require safety protocols to prevent contamination from these pathogens. In this work, we investigated the efficacy of using UV-C irradiation for the purpose of sterilizing an important plant pathogenic bacterium, Erwinia pyrifoliae, in a laboratory setting. For the test, the pathogen (1.71 × 108 CFU/ml) was inoculated on the surface of Potato Dextrose Agar (PDA) and the inoculated media were placed on a work surface in a biosafety cabinet (Class 2 Type A1) as well as on three different surfaces located within the laboratory: a laboratory bench, a laboratory bench shelf, and the floor. All the surfaces where the media were placed were in range of the UV-C beam projected by the UV lamp installed in the ceiling of the BSL 2 Class biosafety laboratory. Measurements of the reduction rate of bacteria under UV-C irradiation were conducted at different time intervals: after 10 minutes, 30 minutes, 1 hour, 2 hours, and 3 hours, respectively. The reduction rate of bacteria ranged from 90% to 99% after 10min irradiation, from 97.8% to 100% after 30 minutes of irradiation, from 99.1% to 100% after 1 hour of irradiation, and from 99.99% to 100% after 2 hours of irradiation. After 3 hours of irradiation, the pathogen was completely killed in all the test conditions. In the cases of the laboratory bench and the shelf of the laboratory bench, the effectiveness of UV-C irradiation differed slightly between the site where the bacteria located vertically under the lamp and the site where the bacteria were located 1 meter away horizontally from the site under of the lamp.

본 연구는 종자의 높은 유지 함량으로 인해 식･약용 및 바이오에너지 원료 소재로 활용 가치가 높은 문관과(Xanthoceras sorbifolium)의 부정아 유도를 통한 기내 재분화 기술을 개발하기 위해 수행되었다. 미성숙 종자 절편을 0.5 mg l-1 6-benzylaminopurine (BA)이 포함된 MS 배지에 치상하였을 때 부정아 유도율은 68.6%로 나타났으나, 성숙 종자 절편을 이용하였을 때 상대적으로 매우 낮은 비율(2.86%)로 유도되었다. 부정아 유도를 위해 싸이토키닌류의 종류를 달리하여 치상하였을 때 thidazuron (TDZ)과 BA가 포함된 배지에서 70% 이상이 유도되었다. 유도된 부정아의 증식을 위해서 절간을 BA와 TDZ이 혼용 처리된 배지에 치상하였을 때 최대 94.29%까지 신초 유도율이 증가되는 것을 확인하였다. 이 후 유도된 신초는 정상적인 발근과정을 거쳐 식물체로 재분화되었다. 본 연구에서 제시된 문관과의 부정아 유도 방법을 이용하여 안정적인 종묘확보에 활용될 수 있을 것이다.

Bolboschoenus planiculmis has been acknowledged as a key species in whooper swans (Cygnus cygnus) habitat by providing food for this migratory waterfowl. B. planiculmis wetlands are being degraded by water shortages and salinization caused by anthropogenic activities and climate changes. In 2004, the distribution of B. planiculmis in the tidal flats of the Nakdong Estuary was 2,475,568 m2, and in 2021, the distribution area was 798,731 m2, which decreased by 32.3%. In order to restore the degraded B. planiculmis wetlands, shoot transplantation and seed sowing were tentatively used in three places with different salinity and water levels. The average density per unit area in September at the optimal growth period after planting were A (fresh water level 50 cm) 58±15.65 m-2, B (brackish water level 0~5 cm) 188±63.83 m-2, C (brackish water level 0 cm or less) 188±45.13 m-2. The tubers were observed as A 0 g dw m-2, B 25.32±2.94 g dw m-2, and C 13.39±0.91 g dw m-2. Tubers were distributed in the soil, with only 3.0% at the 10~20 cm depth but 97.0% at the 0~10 cm depth. In contrast, the germination rate of B. planiculmis seeds was observed to be 0%. Results of this study provide technical support for the restoration of B. planiculmis wetland and the improvement in the quality of whooper swans habitat.

Chinese cabbage or pakchoi (Brassica Rapa subsp. Chinensis) is one of the most popular vegetables in Korea. It is grown and consumed throughout the year because it has many benefits. This study reports on the growth performance of pakchoi grown in the hydroponic system using a Food Juke Box (FJB) under different light compositions. The chlorophyll content (CC), root fresh weight (RFW), and shoot fresh weight (SFW) were measured. Further, various vegetation indices (VIs), such as modified chlorophyll absorption in reflectance index (MCARI1), normalized difference vegetation index (NDVI), optimized soil-adjusted vegetation index (OSAVI), and structure insensitive pigment index (SIPI), were employed to predict SFW. The study results indicated a significant increase in the CC of pakchoi with different light treatments compared with the control. The CC was highest under treatment with 70% white light, 20% blue light, and 10% red light (T1) than under treatment with 50% white light, 30% blue light, and 20% red light (T2). All used VIs showed significant differences with different light combination treatments. There was no significant change in RFW and SFW between the control and T1. However, a significant reduction in RFW and SFW was observed in T2 compared with the control. A comparison between T1 and T2 demonstrated that RFW and SFW were increased by 23% and 25%, respectively, compared with those in T2. The correlation result showed that SFW had a significant positive correlation with RFW (0.76***). Further, a significant negative correlation was observed with OSAVI (−0.25*), MCARI1 (−0.54***), and CC (−0.19*). In conclusion, our findings implied that different light combinations in pakchoi affected the photosynthetic pigments although they did not improve SFW. This research will pave the way for pakchoi production in hydroponics using smart farming FJB. This will further promote plant development, especially for domestic consumption, and help fulfill the growing demand for leafy vegetables.

프로문학이 갖는 정치성은 어떤 스펙트럼을 보여줄 수 있을까? 蔣光慈는 기존의 평가(혁명+연애의 클리셰)와는 달리 색다른 소재와 콘셉트를 시도하여 프로문학의 地境을 넓혀간 작가임을 기억할 필요가 있다. 존재의 외연을 확장하는 일. 감정의 파 동이 일어나는, 가장 내밀한 실존으로의 침잠. 이 모순된 방향의 움직임이 蔣光慈의 서사에서는 동시에 포착된다. 蔣光慈는 혁명의 파고를 관통하면서 첨예한 아이덴티티의 ‘경계’를 경험했던 문제적 인물들을 그려낸다. 주류 서사와 비주류 서사가 공존 하는 蔣光慈의 세계는 좌익문학사에서 독특한 위치를 점한다. 무엇보다 혁명의 관습 과 윤리가 힘을 잃는 내면의 깊은 곳, 그곳에서 마주하게 되는 공허함은 혁명 서사의 圖式을 뛰어넘는 어떤 울림을 선사한다. 본고는 그 울림과 함께 ‘이데올로기’를 초월하는 ‘개체’의 존립 가능성을 蔣光慈의 『菊芬』 속에서 탐색해 보고자 한다.

The evergreen oak tree, Quercus myrsinaefolia Blume, is not only economically important for wood, medicine, landscape trees, etc., but also becoming more important in terms of ecology due to climate change. However, asexual reproduction was difficult, so this study was conducted to establish the optimum conditions for micropropagation by shoot multiplication. The surface sterilized seeds of Q. myrsinaefolia were successfully germinated in WPM basal medium. BAP (1.0 mg/L) treatment was most effective for inducing multiple shoots. The highest induction rates of adventitious roots from the multiple shoots was shown in the treatment of 1.0 mg/L NAA. Both MS and WPM medium were most effective for growth of multiplied plantlets. For ex vitro acclimatization, the survival rates of multiplied plantlets were 100% in vermiculite and commercial soil. The results of this study can be used for proliferation and supply, and establishment of ex situ conservation of Q. myrsinaefolia elite trees.

Plant regeneration from stems and leaves was carried out for the growth of biomass, hybrid poplar(Populus alba x P. glandulosa)clone, which has various uses for plant purification. Callus was well induced when stem and leaf tissues were cultured in 1.0 mg/L 2,4-D containing MS medium. Shoot regeneration was best induced by zeatin among growth regulators, and Plant regeneration was more regenerated in leaf-derived callus than in stem-derived callus. The growth of regenerated shoots at high and low concentrations of zeatin was similar to that of the control at low concentrations. As the concentration of growth regulators increased, the growth of regenerated shoots showed a big difference among individuals. Hybrid poplar showed color variation of plant stem in medium containing high concentration of growth regulator. Regenerated individuals were in vitro rooted in MS medium containing 0.5 mg/L IBA after 2 weeks of culture. and transferred to the greenhouse for acclimatization. The study is believed to be widely used for the induction of in vitro variants through organogenesis.

This study was conducted to determine the applicability of the shoot growth model to cut roses grown at high temperatures. Two cultivars of cut roses, ‘Antique Curl’ and ‘Beast’, were cultivated in growth chambers set to night/day temperatures of 24/20°C for the control group and 32/28°C for the treatment group. The shoot growth model, which resulted in high coefficients of determination (R2 = 0.80 and 0.62, respectively, for ‘Antique Curl’ and ‘Beast’), was a good predictor of the decrease in the growth of rose shoots for both treatment groups. Although the model produced good results for both the control and treatment groups of ‘Antique Curl’, the accuracy of the model can be improved by using the modified leaf area at the harvest stage. From these results, the model was confirmed to perform well for predicting a decrease in productiveness and quality in cut roses cultivated during summer under high temperatures.

An efficient method for in vitro propagation and growth of the wild garlic(Allium ochotense Prokh.) was established. Bulbs of wild garlic were collected from Ullung Island, Korea, and the growth pattern of plantlets on various culture media was observed. High growth of shoot was obtained on LP, NN and N6 medium. The growth media supplemented with 3%(w/v) sucrose was found optimal for shoot growth. After 10 weeks multiple shoots were observed in the plantlets growing on the medium containing 1.0 mg/l of zeatine and 0.1 mg/l NAA. Roots were induced directly at the base of the shoot in all treatments. The medium with 2.0 mg/l of IBA proved to be the best rooting medium. The studies of this kind may be used to develop strategies for large-scale propagation of elite wild garlic.

Dianthus caryophyllus (carnation) is a globally important ornamental plant. Tissue culture techniques have been used for the commercial production of carnation; however, the micropropagation of carnation has been impeded due to the occurrence of hyperhydricity during the shoot multiplication process or micropropagation stage 2. In the present study, the effects of different concentrations of rare earth elements on the reduction of hyperhydricity in micropropagated carnation were investigated. Nodal explants of D. caryophyllus ‘13827’ were cultured on Murashige and Skoog medium containing 1.0 mg·L-1 6-benzyladenine and 0.5 mg·L-1 indole-3-acetic acid with 3.0% (w/v) sucrose and 0.8% (w/v) agar (control medium). The medium was supplemented with lanthanum nitrate, La(NO3)3, cerium nitrate, Ce(NO3)3, or neodymium chloride, NdCl3 at a concentration of 0.05, 0.1, or 0.15 mM. Hyperhydricity was observed in 68.9% of the cultures produced on the control medium. The lowest percentage (42.2%) of hyperhydricity was observed in plants propagated on the medium supplemented with 0.05 mM Ce(NO3)3. Furthermore, the soluble protein concentration was higher in both the non-hyperhydric and hyperhydric plants produced on the medium supplemented with 0.05 mM Ce(NO3)3, whereas the activity of catalase, guaiacol peroxidase, and ascorbate peroxidase was lower in comparison with the media lacking Ce3+. The results of this study suggest that supplementation of the culture medium with 0.05 mM Ce(NO3)3 alleviates oxidative stress and reduces hyperhydricity in carnation during the adventitious shoot multiplication stage.

This study examined the acute toxicity of Vital-Shoot containing reduced glutathione in male and female ICR mice. Mice were intraperitoneally injected the drug at dose levels of 0, 250, 500, 1,000 and 2,000 mg/kg body weight (BW) for single-dose toxicity test. There were no statistical differences in BW changes between the control and all treated groups. Based on hematological and blood biochemical analyses, the drug did not affect all parameters. In addition, markers for liver and kidney functions did not meaningfully change in all treated groups. Since there were no adverse effects from the drug in a single-dose intraperitoneal toxicity test, it was concluded that the lethal dose 50 (LD50) of Vital-Shoot is estimated to be >2,000 mg/kg BW.