본 연구는 식물공장에서 아쿠아포닉스와 수경재배에서 재배된 딸기의 무기양분 흡수율, 생육, 수량을 비 교하고자 수행되었다. 양어는 비단잉어(Cyprinus carpio) 12마리를 수조(W 0.7m × L 1.5m × H 0.45m, 472.5L)에 367.5L 물을 채운 후 입식하였고 5.44kg·m-3 밀도로 사육하였다. 딸기 모종 30개체는 펄라이트를 채운 포트에 정식 하여 아쿠아포닉스 시스템 베드(W 0.7m × L 1.5m × H 0.22m)에 장착하였고, 모종 30개체는 네트포트에 정식한 후 담액수경(DFT)시스템 베드(W 0.7m × L 1.5m × H 0.22m)의 아크릴판(140 cm × 60 cm, Ø80 mm)에 장착하였다. 재 배기간 동안 아쿠아포닉스 수조액의 pH와 EC는 각각 4.3－6.9, 0.32－1.14dS·m-1 수준이었고, 수경재배는 각각 5.1 －7.5, 1.0－1.8dS·m-1이었다. 아쿠아포닉스 수조액의 NO3-N와 NH4-N 농도는 수경재배보다 각각 약 3.6, 2.2 me·L-1 높았다. P, Ca, Mg, S 농도는 수경재배보다 각각 약 0.76, 3.1, 0.8, 0.9me·L-1 높았으며, K와 Fe는 각각 약 0.8me·L-1, 0.5mg·L-1 낮았다. 딸기 잎의 무기이온 함량은 두 재배 처리 간 유의차가 없었으며 엽내 K 함량은 적정 범위를 보였 다. 정식 후 58과 98일 사이에 아쿠아포닉스에서 재배된 딸기의 T-N와 P 흡수율은 수경재배보다 각각 1.5, 1.9배 높 았고 K 흡수율은 유의차가 없었다. 개체당 과실수는 아쿠아포닉스에서 수경재배보다 유의하게 많았으며, 상품과 생체중, 건물중, 과실의 과장과 과폭은 수경재배에서 아쿠아포닉스보다 높았다. 결과를 종합하면, 아쿠아포닉스에 서는 수조액의 물고기 배설물과 먹이 잔여물에 의한 고체 입자의 비료성분을 지속적으로 가용하여 활용한다는 것 을 알 수 있다.

Along with other heavy metals, arsenic (As) is one among the substances most harmful to living organisms including humans. Owing to its morphological similarity to phosphorus, the uptake of As is influenced by photosynthesis and the phosphorus uptake pathway. In this study, we varied arsenic exposure and light intensity during nutrient solution cultivation of lettuce (Lactuca sativa L.) to determine the effect of these two factors on arsenic uptake, lettuce growth, and electron transfer in photosystem II. In the treatment exposed to 30 μmol L-1 of arsenic, the shoot arsenic concentration increased from 4.73 mg kg-1 to 18.97 mg kg-1 as the light intensity increased from 22 to 122 μmol m-2 s-1. The water content and ET2o/RC of the shoots were not affected by arsenic at low light intensity; however, at optimal light intensity, they decreased progressively with arsenic exposure. Increased light intensity stimulated the growth of plant roots and shoots; contrarily, the difference in growth decreased as the concentration of As exposure increased. The results of this study suggest that the effect of As on plant growth is dependent on light intensity; in particular, an increase in light intensity can increase the uptake of As, thereby affecting plant growth and As toxicity.

Crassulacean acid metabolism (CAM) plants use surplus CO2 generated by cooling and heating at night when ventilation is not needed in a greenhouse. Schlumbergera truncata ‘Pink Dew’ is a multi–flowering cactus that needs more phylloclades for high–quality production. This study examined photosynthetic characteristics by the phylloclade levels of S. truncata in a growth chamber and a greenhouse for use of night CO2 enrichment. The CO2 uptake rate of the S. truncata’s top phylloclade in a growth chamber exhibited a C3 pattern, and the second phylloclade exhibited a C3 –CAM pattern. The CO2 uptake rate of the top phylloclade in a greenhouse showed a negative value both day and night, but those of the second phylloclade exhibited a CAM pattern. The stomatal conductance and water–use efficiency (WUE) of S. truncata at both the top and second phylloclades were higher in a growth chamber than in a greenhouse. The WUE of S. truncata in a growth chamber and a greenhouse was higher at the second phylloclade, which is a CAM pattern compared with those of the top phylloclade. The daily total net CO2 uptake of S. truncata was higher in a growth chamber than in a greenhouse. The daily total net CO2 uptake of S. truncata at the second phylloclade had the highest value of 155 mmol·m–2·d–1 in a growth chamber. The night total CO2 uptake of S. truncata at the second phylloclade was 3–fold higher in a growth chamber than in a greenhouse. S. truncata’s second phylloclade exhibited a CAM pattern that uptake CO2 at night, and the second phylloclade, was more mature than the top phylloclade. A multi–flowering cactus S. truncata ‘Pink Dew’ efficiently uptake night surplus CO2 in the proper environmental condition with matured phylloclade.

Arsenic (As) uptake and accumulation from agricultural soil to rice vary depending on the soil environmental conditions such as soil pH, redox potential, clay content, and organic matter (OM) content. Therefore, these factors are important in predicting changes in the uptake and accumulation of As in rice plants. Here, we studied the chemical properties of As-contaminated and/or rice straw compost (RSC)-treated soils, the growth responses of RSC-applied rice plants under As-contaminated soils, the changes in As content of soil, and the relationship between As uptake and accumulation from the RSC-treated soils to the rice organs under As-contaminated soils. Rice plants were cultivated in 30 mg kg-1 As-contaminated soils under three RSC treatments: 0 (control), 12, and 24 Mg ha-1. No significant differences were indicated in the chemical properties of pre-experimental (before transplanting rice seedling) soils, with the exception of EC, OM, and available P2O5. As the treatment of RSC under 30 mg kg-1 As-contaminated soils increased, EC, OM, and available P2O5 increased proportionally in soil. Increased soil RSC under As-contaminated soils increased shoot dry weight of rice plants at harvesting stage. As content in roots increased proportionally with RSC content, whereas As content in shoots decreased under As-contaminated soil at all stages of rice plants. Nevertheless, As accumulation were significantly decreased in both roots and shoots of RSC-treated rice plants than those in the plants treated without RSC. These results indicate that the use of RSC can mitigate As phytotoxicity and reduce As accumulation in rice plants under As-contaminated soils. Therefore, RSC can potentially be applied to As-contaminated soil for safe crop and forage rice production.

This study aims to investigate the effects of exogenous succinic acid (SCA) on Brucella (B.) abortus infection in macrophage RAW 264.7 cells and ICR mice. Firstly, the in vitro experiment was conducted by MTT cytotoxicity and bacterial internalization assay to evaluate the uptake of B. abortus into macrophage cells. Two non-cytotoxic concentrations of SCA demonstrated attenuated invasion of Brucella into macrophages at 30 and 45 min post- infection (pi). Secondly, ICR mice were treated with SCA and infected with B. abortus. On day-14 pi, spleen and blood serum were collected to evaluate the bacterial burden and total spleen weight as well as the production of cytokine/chemokine, respectively. The results showed that SCA treatment promoted bacterial growth and reduced the total spleen weight in mice. Furthermore, SCA treatment increased the level of IL-10 cytokine in the sera, while dampening the production of MCP-1 chemokine compared to the control. The results of bacterial load in spleen and spleen weight together with cytokine/chemokine production profile in the sera indicated that SCA induced the host anti-inflammatory response which is beneficial for the survival of Brucella. Therefore, these findings suggest that SCA contributed to host immunity against Brucella infection and the emerging potential topic-immunometabolism should be invested for further investigations.

Biological phosphorus removal is accomplished by exposing PAO(phosphorus accumulating organisms) to anaerobic-aerobic conversion conditions. In the anaerobic condition, PAO synthesize PHB(polyhydroxybutyrate) and simultaneously hydrolysis of poly-p resulting phosphorus(Pi) release. In aerobic condition, PAO uptake phosphorus(Pi) more than they have released. In this study, cyanobacteria Synechococcus sp., which is known to be able to synthesize PHB like PAO, was exposed to anaerobic-aerobic conversion. If Synechococcus sp. can remove excess phosphorus by the same mechanism as PAO, synergistic effects can occur through photosynthesis. Moreover, Synechococcus sp. is known to be capable of synthesizing PHB using inorganic carbon as well as organic carbon, so even if the available capacity of organic carbon decreases, it was expected to show stable phosphorus removal efficiency. In 6 hours of anaerobic condition, phosphorus release occurred in both inorganic and organic carbon conditions but SPRR(specific phosphorus release rate) of both conditions was 10 mg-P/g-MLSS/day, which was significantly lower than that of PAO. When converting to aerobic conditions, SPUR(specific phosphorus uptake rate) was about 9 mg-P/g-MLSS/day in both conditions, showing a higher uptake rate than the control condition showing SPUR of 6.4 mg-P/g-MLSS/day. But there was no difference in terms of the total amount of removal. According to this study, at least, it seems to be inappropriate to apply Synechococcus sp. to luxury uptake process for phosphorus removal.

The purpose of this study was to investigate the toxic effects of zinc in collembolan Paronychiurus kimi at the individual (survival and juvenile production) and population (population growth and age structure) levels after 28 days of exposure in artificially spiked soil. These toxic effects were interpreted in conjunction with the internal zinc concentrations in P. kimi. The EC50 value for juvenile production based on the total zinc concentration was 457 mg Zn kg-1 dry soil, while the LC50 value for adult survival and ri=0 value for population growth were within the same order of magnitude (2,623 and 1,637 mg Zn kg-1 dry soil, respectively). Significant differences in adult survival, juvenile production, and population growth compared with the control group were found at concentrations of 1,500, 375, and 375 mg Zn kg-1 dry or higher, respectively, whereas significant differences in the age structure, determined by the proportion of each age group in the population, were observed in all treatment groups. It appeared that the internal zinc level in P. kimi was regulated to some extent at soil zinc concentrations of ≤375 mg Zn kg-1 dry soil, but not at high soil zinc concentrations. These results indicate that, despite zinc being regulated by P. kimi, excess zinc exceeding the regulatory capacity of P. kimi can trigger changes in the responses at the individual and population levels. Given that population dynamics are affected not only by individual level but also by population level endpoints, it is concluded that the toxic effects of pollutants should be assessed at various levels.

본 연구는 담녹조강 Tetraselmis suecica와 T. tetrathele의 인산염 흡수에 미치는 발광다이오드의 파장의 영향을 조사하였다. 파장은 청색 발광다이오드(LED; 450 nm), 황색 LED(590 nm), 적색 LED(630 nm) 그리고 형광램프(control)이며, 조도는 100 μmol m-2 s-1로 주사하였 다. T. suecica와 T. tetrathele의 최대흡수속도(ρmax)는 적색 LED에서 6.35 pmol cell-1 hr-1와 9.85 pmol cell-1 hr-1로 나타났으며, 반포화농도(Ks)는 9.43 μM와 21.2 μM로 나타났다. 이는 적색 LED 아래에서 T. suecica와 T. tetrathele가 다른 파장보다 영양염에 대한 친화성이 높다는 것으로 의미한다. 따라서 경제성 및 생산성 향상을 위한 Tetraselmis 배양시스템(광배양기)의 최적 광원은 낮은 영양염 상태에서도 높은 생장속도를 보이는 적색 LED가 적합할 것으로 판단된다.

배양액의 농도 조건과 품종별 양수분 흡수특성을 구명하여 장기 수경재배를 위한 기초자료를 획득하고자 연구를 수행하였다. 시험 품종으로 토마토 대과종으로는 적색계인 ‘대프니스’와 도색계인 ‘수퍼도태랑’ 소과종으로는 ‘미니찰’ 품종을 이용하였다. 담액재배하였으며 배양액의 EC를 1.0dS·m-1, 2.0dS·m-1, 3.0dS·m-1, 4.0dS·m-1로 다르게 공급하였다. 배양액의 EC가 높은 처리에서 초기에는 엽면적, 생체중이 감소하였으며 염류장해가 발생하면서 생육(초장, 엽면적, 경경, 생체중)이 불량해졌다. 배양액의 EC가 높을수록 수분흡수가 적었다. 수분흡수량은 1차에서는 품종별 차이가 뚜렷하지 않았으나 2차 조사에서는 ‘대프니스’가 EC 2.0dS·m-1 이상에서도 수분흡수가 크게 감소하지 않았으나 ‘수퍼도태랑’은 높은 EC 처리에서 수분 흡수가 감소하였다. 배양액의 EC가 낮은 처리에서 무기이온의 흡수는 N, P, K는 급액농도 보다 높게 흡수된 반면에 Ca, Mg, S는 흡수율이 낮았다. 배양액의 EC가 높은 처리에서는 대부분의 이온이 초기 투입농도의 50% 이하로 흡수되었다. 따라서 EC가 낮은 처리가 높은 처리 보다 흡수되고 남은 배양액의 이온간 불균형이 심하였다. 품종 간에는 ‘대프니스’가 저농도에서 흡수량이 많고 고농도에서는 흡수량이 적어 불량한 양분조건에서 양분을 효율적으로 이용하는 품종이었 으나 과잉 흡수된 양분으로 인한 장해 증상은 가장 심하게 나타내었다.

Magnetic resonance imaging (MRI) technique has recently been applied in agriculture and food science. It has potential to visualize internal structure of foods in nondestructive way and provides spatial information which could make reconstruct of three-dimension. MRI system also has great advantage for tracking water in beans or seeds. This study intends to analyze the changes of water distribution of coffee beans during water up-taking process. Samples used in the experiment were ‘Robusta’ coffee beans (Vietnam G1, Scr18 polish). A 30mm diameter plastic disposable test tube was used to hold bean during water up-take process. The oil column was placed next to the tube to get and to use as a reference signal. The magnetic resonance imaging system which installed at Institution for Agricultural Machinery & ICT Convergence in Chonbuk National University was used to obtain magnetic resonance (MR) image data sets. 30 images are included in each image data sets with ‘Axial’ image plane direction. In experiment, we used ‘Gradient Echo 3D’ pulse sequence. The water uptake process was performed for 150 minutes and acquired image data sets in every 10 minutes. This study showed some coffee beans which extracted as region of interest (ROI) from each images to study the water uptake process.

This study was performed to investigate how changing the period of light and dark influences the vegetative growth and the photosynthesis of Doritaenopsis Queen Beer ‘Mantefon’. Clones of Dtps. Queen Beer ‘Mantefon’ at 4-month-old stage were grown in a closed-plant factory system with four different light/dark cycles; 06/06 h, 08/08 h, 10/10 h, and 12/12 h. Temperature and relative humidity were set at 28oC and 80%, respectively, with a photosynthetic photon flux density of 160 ± 10 μmol·m-2·s-1. Repetitive measurements showed that the leaf length and the leaf width were the longest under 12/12 h closely followed by 10/10 h. The fresh weight and the dry weight of leaves and roots were the heaviest at 10/10 h treated samples. Different CO2 uptake patterns were observed from different light/dark cycles. Under 10/10 h and 12/12 h treatments, the CO2 uptake started at early dark period. When the light/dark cycles were shortened to 06/06 h and 08/08 h, the CO2 uptake started at the middle of dark period. Total CO2 uptake amounts were the highest under 12/12 h treatment followed by 10/10 h, 06/06 h, and 08/08 h treatments. Quantitative measurements showed that the vegetative growths under 10/10 h treatment were comparable with that of 12/12 h treatment. These studies indicated that manipulating light/dark can modify the photosynthesis patterns and vegetative growth of Dtps. Queen Beer ‘Mantefon’, resulting in the reduction of the production period.

산림을 통한 대기 중 이산화탄소 고정은 온실가스를 감축 시키는 비용 효과적인 수단으로 알려져 있다. 산림의 조성 및 경영, 산림전용 방지, 목질 바이오매스의 순환 이용 등을 통해 탄소흡수량 증진사업을 진행할 수 있으며, 이는 사회 공헌활동이나 탄소마케팅, 대중의 기후변화 인식 확대 등에 활용되고 있다. 국내에서도 기업과 국가기관의 숲가꾸기와 같은 사회공헌활동을 시각화하고, 해외의 자발적 탄소시장 과 연계하며, 국내 배출권 거래제도의 도입 시 온실가스 배 출 의무감축량의 상쇄 수단으로 활용하기 위한 목적으로 산림 탄소상쇄 프로그램 이용에 대한 관심이 높아지고 있 다. 국내기업인 유한킴벌리는 다양한 사회공헌활동을 진행 하고 있으며, 2016년 환경보호 시민단체인 (사)생명의숲 국 민운동과 함께 충주를 대상으로 공존숲 프로젝트를 진행하 였다. 공존숲은 숲과 사람의 공존을 비전으로 설정하고, 기 존의 숲에서 더 나은 숲으로 복원 유도 및 기후변화에 대응 하기 위한 산림탄소상쇄 숲 조성을 목표로 하고 있다. 따라 서 본 연구는 충주 공존숲을 대상으로 도시림의 탄소흡수량 증진을 위한 관리계획을 제시하고자 한다. 연구는 충청북도 충주시 주덕읍 대곡리 산71-1, 산71-67 (총면적 647,306.4㎡)에 위치한 산림을 대상으로 진행되었 으며, 현존식생도 조사, 산림군락조사 등을 진행하여 대상 지 산림 특성을 우선 파악하고, 조사된 산림 특성과 이용계 획을 기초로 하여 기후변화 대응을 위한 산림탄소상쇄 숲 조성 계획을 수립하였다. 충주국유림관리소에서 제공받은 기존 소반을 현존식생을 바탕으로 통합, 세분하여 총 8개로 구획을 구분하였으며, 수종별 지위지수를 산정, 이를 이용 해 산출한 수피포함 수간재적과 기타 계수를 적용하여 탄소 저장량을 산출하였다. 사업 대상지에 산림경영 사업을 시행하지 않았을 경우 탄소경영 사업을 통해 인정받을 수 있는 실질 탄소흡수량을 베이스라인 흡수량(배출량)이라 하며, 기준벌기령에 따른 수확벌채와 그 후 재조림되는 상황을 고려하였다. 법정 벌 기령에 의해 벌채가 이루어지면, 누적된 탄소저장량이 일부 혹은 전부 소실되며, 소실된 이후 재조림에 의해 탄소저장 량이 다시 증가하게 된다. Climate Action Reserve(2012) 방법에 따른 10년간에 베이스라인 흡수량은 3,692tC로 산 정되었다. 사업 대상지에 산림경영 사업이 시행될 경우의 흡수량을 프로젝트 흡수량이라 하며, 벌기령을 연장하고 생 육이 불량한 곳에 수종을 조림하고, 산림을 보전하는 등 탄 소저장량을 증가시키는 경우이다. 시간이 흐를수록 총 탄소 저장량은 꾸준히 증가하게 된다. 대상지 이용 계획 및 경관 향상을 목적으로 하여 대상지 상황과 경관에 따른 각 구획 별 주요수종에 대한 벌기령 연장과 보식을 통해 사업수행 10년간 유지 및 보전할 수 있는 탄소흡수량을 산정하였다. 기존 현존식생 유지관리 및 수종 보식에 따른 산림탄소상쇄 숲 조성사업을 진행하게 될 경우 프로젝트 흡수량은 1년차 에 4,148tC, 5년차에 4,933tC, 10년차에 5,663tC로 산정되 었다. 프로젝트 흡수량에서 베이스라인 흡수량을 제외한 10 년간 이산화탄소 순흡수량은 7,228tCO2(연간 723tC)으로 산정되었다. 대상지 이용 계획에 타당하도록 기존 현존식생 유지, 수 종 보식 등을 진행하여 산림탄소상쇄 숲을 조성하였을 때 기존 산림을 유지하는 것보다 약 2,000tC 가량 산림탄소 흡수량이 증가하게 된다. 기업은 탄소상쇄 숲 조성을 포함 한 새로운 사회공헌형 활동을 통해 지역의 산림을 보다 나 은 산림으로 조성하며, 지역 주민은 이를 통해 생태계서비 스를 제공받고 기후변화 및 산림에 대한 인식 증진에 영향 을 받게 된다. 따라서 충주 공존숲은 산림탄소상쇄 숲 조성 을 통해 산림 복원 및 기후변화 대응에 있어 새로운 도시림 관리계획 방안을 제시할 수 있을 것으로 사료된다.

The objective of this study was to investigate the effects of combinations of tofu paste and non-starch polysaccharides (NSP) on the oil uptake reduction (OTR) of deep-fat fried cake doughnuts. OTR agents were tofu paste (from grinding tofu with deionized water, followed by passage through a 60 mesh sieve), and five neutral and nine anionic NSPs. A control doughnut (without tofu paste or NSP), tofu doughnut (with tofu paste) and NSP-tofu doughnut (with tofu paste and NSP) were prepared. The moisture and total lipid (TL) content, cross-section image, color characteristic, and specific volume were measured. The tofu and NSP-tofu doughnuts exhibited higher moisture and lower TL content than the control. OTR was 10.8% for the tofu doughnut, and between 13.2% and 41.2% for the NSP-tofu doughnut. The highest OTR (41.2%) was found in the NSP-tofu doughnut with a combination of tofu paste and sodium alginate (NaA). The specific volume of the NSP-tofu doughnuts with combinations of tofu paste with NaA (2.5 mL/g), locust bean gum (2.5 mL/g), and κ-carrageenan (2.4 mL/g) was very close to that of the control (2.6 mL/g). Considering the OTR and specific volume of doughnuts, the combination of tofu paste and NaA would be most effective in reducing the oil uptake of doughnuts during deep-fat frying.

This study investigated the effect of high concentration of free ammonia on microalgal growth and substrate removal by applying real wastewater nitrogen ratio. To test of this, the conditions of free ammonia 1, 3, 6, 9, 12, 15 mg-N/L are compared. After 3 days of incubation, algal growth of Chlorella vulgaris and carbon removal rate are respectively lower in the reactors of FA 12, 15 mg-N/L compared to the others. This indicates that the high concentration of free ammonia, in this case, above 12 mg-N/L, has negative effect on algal growth and metabolic activity. Also, high concentration of free ammonia causes the proton imbalance, ammonium accumulation in algae and has toxicity for these reasons. So, we have to consider free ammonia in applying the microalgae to wastewater treatment system by the way of diluting wastewater or controlling pH and temperature.