The scientific questions arising from the ability of certain species, but not others, to massively regenerate their bodies are among the most fascinating and challenging confronting modern cell and developmental biologists today. The tremendous implications of this research area for human medicine and tissue engineering are obvious. Yet many other animals exhibit robust regenerative capabilities, including “lower” vertebrates such as amphibians, and invertebrates such as echinoderms, flatworms and annelids. In the extreme case, some species can reproduce vegetatively indefinitely. Such animals must contain the operational equivalent of immortal, totipotent somatic stem cells. From invertebrates to the higher vertebrates, their metabolic pathway, developmental regulatory genes, and intercellular signaling pathways are evolutionary conserved. With these, study on regeneration is an ingenious, powerful model system for studying the post-embryonic development and innate immunity mechanisms. Here, I will discuss the processes of immunemediated gut injury and regeneration using annelid regeneration model system.

Insect cuticular melanization is regulated by the prophenoloxidase (proPO)- activating system, which is also involved in the innate immune reaction. Here, we demonstrate how the differentiation of the proPO-activating system is regulated toward a cuticular melanization or innate immunity function in silkworm (Bombyx mori) pupae. Our results indicate that the differential and spatial regulation of key components, such as the proPO-activating factor, tyrosine hydroxylase, and porPOs, primes the proPO-activating system for either cuticular melanization or innate immunity. This dual strategy for cuticular melanization in development and innate immunity upon infection demonstrates a two-pronged defense mechanism that is mediated by the priming of the proPO system.

L. rhamnosus GG 유산균은 1983년 건강한 성인의 장관에서 분리된 이래 인체의 수많은 건강효과에 관한 연구들이 진행되었다. 전세계의 연구자들이 이 프로바이오틱 유산균에 관심을 갖게 되었고, 인체에 대한 다양한 임상시험과 흥미로운 연구들이 진행되었다. 지난 20여 년간 프로바 이오틱 시장이 급격히 성장했음에도 불구하고, L. rhamnosus GG는 아직도 세계에서 가장 연구가 많이 진행된 유산균이다. 비록 in vitro와 실험적 연구들을 인체와 직접적으로 관련시킬 수는 없지만, 위의 연구들을 통해 L. rhamnosus GG가 장관에서 병원성 미생물을 억제하고, 이들이 혈관이나 세포조직으로 침투하는 것을 억제하며, 시스템적인 또는 국소적인 면역반응을 증강시키고, 감염 억제와 감염으로부터의 회복을 빠르게 하는 등의 역할을 하고 있음을 알 수 있다.

This study analyzed effects of different sound treatments in frequencies and intensities on digestion and immune physiological processes of the beet armyworm, Spodoptera exigua larvae. Without effect on egg hatch, sound treatments with 100-5,000 ㎐ at 95 ㏈ suppressed feeding behavior and inhibited a digestive enzyme activity. In addition, two dimensional electrophoresis of midgut luminal proteins indicated a marked difference of the sound-treated larvae. In response to 5,000 ㎐ at 95 ㏈, larvae showed a significant decrease in hemocyte nodule formation against fungal challenge along with significant suppression in phospholipase A₂ activity in hemocyte and plasma. With increase of sound frequencies, the treated larvae showed an enhanced susceptibility to insecticides. Such sound frequency effect was significantly modulated with different sound intensities. These results suggest that sound treatment may give adverse stress to physiological processes of S. exigua larvae and may be applied to a nonchemical insect pest control.

This research focuses on the study about the mugwort moxibustion and the manufacture of moxacautery instrument, which has been highly regarded in terms of alternative medicine and preventive medicine. The research has its purpose to study moxacautery instrument, which the far infrared ray, magnetic force, essential amino acids, good quality of protein, and vegetable fat, can be absorbed into the system of through the skin. The technical background of the research offers the characteristics of the far infrared ray, relationship between the far infrared ray and the human body, the role of the main body of moxacautery instrument, the function of the cover, and the features of the undercover. The research also shows the manufacture process of moxacautery instrument and natural nano materials based on mineral and bamboo charcoal. In the light of the excellence of mugwort moxibustion there is not much need to explain the way of using moxacautery instrument and treatment coverage. This research suggests the coverage of mugwort moxibustion- neurosis, metabolism, malnutrition, endocrine diseases, respiratory diseases, tuberculous diseases, digestive diseases, circulatory diseases, malignant[vicious] tumor, urinary diseases. The research also suggests three spot on the body important for moxacautery to strengthen the immune function of the body and seven spot on the body important for moxacautery to keep health and to treat the body.

Background : Cirsium setidens is a perennial wild herb that belongs to the Asteraceae family. It grows in the mountainous region of Gangwon-do in Korea and is also called gondre. The bioconversion technology applied in this study has the same meaning as biosynthesis, biocatalyst, etc., and refers to a technique for producing desired products from precursors using enzymatic functions of microorganisms. Therefore, useful microorganisms having immunological activity were selected and Cirsium setidens fermentation products were prepared by applying bioconversion technology. And fermented product extracts were prepared to consider as a good sources of natural immune enhancement and functional food ingredients. Methods and Results : Lactobacillus fermentum, Lactobacillus plantarum, Saccharomyces cerevisiae, Weissella cibaria, and Lactobacillus plantarum were used as isolates from traditional foods. The fermentation product was set to a condition that the inherent physical properties did not change and did not generate a unique odor during fermentation. Cirsium setidens was fermented at 37℃ for 24 hours. And the fermented material was sterilized at 9 0℃ for 1 hour and then dried at 70℃ and pulverized. The contents of pectolinarin and pectolinarigenin, which are non - glycosides, were analyzed before and after fermentation using HPLC. Also NO production was measured in RAW264.7 cells after extract treatment at various concentrations using Griess reagent kit . The content of pectolinarin was increased in fermented Crisium setidens before fermentation, but the content of pectolinarigenin was increased after fermentation. In addition, the water extract of the fermented material accelerated the NO production compared to the pre - fermented material. Conclusion : As a result, relatively high immunostimulating effect were observed in dried Crisium setidens after fermentation, and it was confirmed that it could be a ingredient material for health functional food.

본 연구는 키토산올리고당의 음수 내 급여가 따른 육계의 혈액성상, 면역력 및 항산화효소계에 미치는 영향을 알아보기 위해 1일령 Arbor Acre종 약 2만 8천수를 대조구와 키토산 음수 급여구로 나누어 5주 동안 실시하였다. 육계의 혈액 성상에서 Glucose의 농도는 대조구보다 키토산올리고당 음수 급여구가 유의적으로 감소하였으나, Triglyceride, Total cholesterol, HDL-cholesterol 및 LDL-cholesterol 농도에는 대조구와키토산올리고당 음수 급여구간에 차이를 보이지 않았고 간 손상에 대한 인자인 GOT농도는 시험구간에 차이가 없었지만 GPT농도는 대조구보다 키토산올리고당 음수 급여구가 유의적으로 감소하였다(P<0.05). 혈액 내 면역글로블린 농도 중, IgG 농도는 대조구와 키토산올리고당 음수 급여구간에 차이가 없었으나, IgM 농도는 대조구보다 키토산올리고당 음수 급여구가 유의적으로 증가하였다(P<0.05). 항산화력과 SOD의 농도는 시험구간에 차이가 없었으며, Catalase와 Glutathione peroxidase의 농도는 대조구보다 키토산올리고당 음수 급여구가 유의적으로 증가하였다(P<0.05). 따라서, 키토올리고당의 음수 급여로 인해 육계의 당대사, 면역기능 및 1차 항산화 효소계의 활성을 증가시켜 육계의 강건성에 영향을 미칠 수 있음을 보여주었다.

In the era of systems biology, plant biologists approach any given phenomena, that they have great interests, from different perspectives. Among them, both epigenomic and epigenetic studies give us new insights into plant immune response as well as development. In plants, recognition of invading pathogenic microorganisms by pattern recognition receptor and race-specific resistance protein activates diverse cellular responses to defend plants against pathogen infection. One of well-known immune responses is the transcriptional reprogramming occurring when pathogen infects plant. Chromatin remodeling caused by change of histone marks and replacement of histone variants affects gene expression that is important for immunity. We are focused on unveiling epigenomic and epigenetic regulatory mechanisms of plant immunity. To address these questions, we have collected knockout mutant plants whose genes might be related to histone modifications, and identified several enhanced-immune (eni) mutants and immune-defective (imd) mutants. Here, we will introduce one of mutants showing enhanced disease resistance (EDR) in response to the infection of Pseudomonas. Thus we named it eni2. Both the growth of virulent bacteria, not avirulent derivatives of Pseudomonas syringae, and symptom development were effectively inhibited in the eni2 mutants, compared with those seen in wild type. Unlike to well-known EDR-type mutants, the levels of salicylic acid in the eni2 mutant plants were not different from those in wild type. Thus we suggests a few plausible scenarios about role of ENI2 in plant immune response. To examine these possibilities, we are monitoring transcriptional reprogramming occurring in eni2 mutants through RNA-seq analysis. On the other hand, we have recently initiated the study of tomato small RNAome in order to discover immune-related small RNAs from leaves infected with Pseudomonas syringae via next-generation sequencing technology. Here we present the recent progress in this study.

Disease is one of the significant factors to damage for the crop productivity, including rice. Although there are many methods to avoid from several diseases such as chemical pesticides and biological treatments, it has been appreciated that the most economical and environmentally effective method of disease control is application of resistance genes. A survey (Dardick & Ronald, 2006) reported that plant kinome has a small number of non-RD kinase (nRDK) (4-29% of total kinase), all known or predicted pattern recognition receptors (PRRs) fall into the class. We here introduce a strategy to identify rice resistance genes that are probably encoding PRRs. We selected 130 nRDK genes by combinational analysis of QTL and bioinformatics, 61 of rice mutant lines of 130 candidates inoculated by Xanthomonas oryzae pv. oryzae (Xoo) and Magnaporthe grisea. (M. grisea), and disease progression was monitored. Lesion lengths of the activation mutant lines for nRDK-08 and nRDK-18 genes reduced more than 34% compared to wild type of rice (Dongjin) and other mutant lines. The nRDK-03 and nRDK-17 gene activation rice line had remarkably smaller lesion lengths by M. grisea infection. Our results suggest that a reverse genetic approach using bioinformatics and T-DNA tagging system successfully identified nRDK genes conferring a resistance against Xoo and M. grisea.

현재 자동차나 선박등에 사용하는 직류모터 속도 제어용 스위칭 소자는 스위칭 과전압으로 인하여 때때로 손상되거나 오작동을 일으키기쉽다. 본논문은 dc 모터 제어용 반도체 스위칭소자의 내성특성을 개선한 것이다. 이를 위하여, 먼저 저전압 보호소자인 비리스터나 제너 다이오드 등 가운데에서 스위칭 과전압에 대해 내성을 갖는 이상적인 소자를 선택하기 위해 과전압 보호자의 등가회로를 분석 하였다. 나아가서 국제규정에서 정의된 의사 과전압 파형의 적절한 선택, 스위칭 소자의 손상과정과 손상원인을 규명하고, 순시과전압 전류의 변화에 따른 각각의 임피던스 조건을 고려하여 직류모터 제어용 반도체 스위칭 소자의 내성특성 개선하였다.

The method of Electromagnetic Wave Immunity Test is continuously endeavored by IEC and CISPR as international unification, but it has not been established yet. The main reason is why it is difficult to obtain the measurement method and normalization of threshold to find out the complicated parameters for Electromagnetic Wave immunity Test. So far, thus, the Electromagnetic Wave Immunity Test is carried out case by case as the electromagnetic environment, which is set up Electromagnetic Wave Immunity Test. This study has developed an algorithm and measurement software suitable for IEC1000-4 Regulations, where a ready-made Amplifier, and Signal Generator have been used and an interconnecting Interface Board has been constructed for connecting with the other apparatuses, i.e., RF Switch, Power Meter, Field Sensor, Pre-Amp., etc. Therefore, we have achieved the aim to develop an automated software using OOP method and the measurement system.