간행물

한국응용곤충학회 학술대회논문집

권호리스트/논문검색
이 간행물 논문 검색

권호

2019 Spring International Conference of KSAE (2019년 4월) 299

Poster Presentation : Pest Control

181.
2019.04 구독 인증기관·개인회원 무료
과일 초파리(Drosophila melanogaster)는 실온 조건에서 한 주기가 12일 전후로, 세대가 짧고 번식력이 높아 일상생활 에서 다양한 피해를 주고 있다. 초파리 방제를 위해 살충제제 형태도 개발되어 있으나 주로 주방이나 음식물 근처에 발생하므로 약제 활용보다 살충 성분을 포함하지 않은 트랩 제품을 사용하는 것이 현 소비 트렌드에 적합한 것으로 보인다. 소비량이 높은 실온 과일 중, 바나나의 초파리 유인도를 확인하고 실질적으로 초파리 트랩 제품의 효능을 검정하기 위해 특정 공간 내(1.5x1.5x1.5 m)에서 초파리 유인도를 확인하였다. 국내 및 해외에 유통 중인 초파리 트랩 제품은 단독 평가 시 바나나 반 송이를 기준으로 과일과 동등한 유인 효과를 나타냈다. 그러나 과일과 함께 평가를 진행하는 경우, 과일에 유도된 초파리에 비하여 제품이 초파리를 유인하는 효과는 현저히 낮은 것으로 확인되었다. 본 결과를 바탕으로, 향후 초파리 트랩 제품 개발 시 실생활에 대한 환경을 상정하여 시험법을 개발할 필요가 있다.
182.
2019.04 구독 인증기관·개인회원 무료
The greater rice weevils, Sitophilus zeamais, are one of the devastating insect pests of stored cereals. Since they reside in stored product, mainly food, synthetic insecticides are not favorable choice to control them, as which botanical pesticides can be alternatives in this particular situation. In the present study, contact and fumigant activity of twenty-three plant essential oils against the adults S. zeamais were examined. To identify the chemical compositions of potent essential oils, Gas chromatography-mass spectrometry was performed. Interestingly, essential oils which showed great contact toxicity did not always produce fumigant toxicity accordingly. Among the twenty-three oils assessed, seventeen oils displayed acute contact toxicity and only six oils exhibited fumigant effect. Cinnamon oil was the most toxic in both fumigant and contact assays (LC50 = 10.02 μL/L air, 0.04 μL/cm2, respectively). Cinnamaldehyde in cinnamon oil and terpinen-4-ol in tea tree and majoram oils were identified as the most abundant components, respectively.
183.
2019.04 구독 인증기관·개인회원 무료
Trialeurodes vaporariorum is an important greenhouse pest worldwide. We compared the efficacy of a new strain of Beauveria bassiana (ARP14) with a commercialized one (GHA) against 2nd instar and 4th instar of T. vaporarioum. Those nymphs were exposed to the fungus in a concentration of 1×108 conidia/mL by leaf dipping method. There was no difference in mycosis rates at any observation time between the two strains in both 2nd (100.0%) and 4th instar (100.0%). Lethal median time (LT50) wasn't different between the two strains in both 2nd and 4th instar. In conclusion, the new B. bassiana strain (ARP14) would be a good candidate of mycoinsecticide for whitefly management.
184.
2019.04 구독 인증기관·개인회원 무료
Minute pirate bug, Orius minutus (L.), is a native predator of many small insects such as aphid, scale, thrips, and mites in Korea. Seven chemical pesticides with lower ecotoxicity being used to control those pests were evaluated for acute toxicity against adult female O. minutus in glass scintillation vial assay. In the 1st batch experiment with three pesticides, flonicamid and buprofezin were least toxic whereas in the 2nd batch experiment with four pesticides spirotetramat and spiromesifen showed least toxicity. The corrected mortality of buprofezin and flonicamid became 100% on 55 and 47 h of exposure in 1st batch and that of spirotetramat and spiromesifen became 100% on 75 and 71 h of exposure in 2nd batch, respectively. In conclusion, flonicamid, buprofezin, spirotetramat, and spiromesifen was least toxic among the seven pesticides tested against adult female of O. minutus.
185.
2019.04 구독 인증기관·개인회원 무료
국내 농작물에 발생하는 주요 해충인 진딧물류의 방제를 위해 기생성 천적을 활용하여 생물적 방제를 하는 방법이 사용되고 있다. 그러나 농작물에 국부적으로 진딧물이 대량 발생할 경우에는 화학적 방제를 사용하는 것이 효과적이다. 따라서 진딧물류의 효율적인 방제를 위해 진딧물 천적에 영향이 적은 저독성 약제를 선발하여 생물적, 화학적 방제의 혼합사용 방안을 마련하고자 한다. 이를 위해 기생성 천적인 B. communis 머미에 대하여 진딧물 등록약제인 스트레이트 외 31가지의 약제를 처리하여 저독성 약제를 선발하였다. 상온에서 B. communis 머미를 사각 페트리디쉬(72×72×100mm, SPL 310075)에 10개씩 넣고, 대상 약제를 추천배수로 희석한 후 분무법으로 살포하였 다. 처리 후 1, 3, 5일 후 B. communis 머미에서 우화한 성충수를 조사하였으며, 3반복으로 실시하였다. 아타라 외 4가지 약제는 머미의 우화율이 50% 이하였으나 나머지 26개 약제는 50% 이상의 우화율이 나타났다.
186.
2019.04 구독 인증기관·개인회원 무료
This study was conducted to survey the insect pests on 30 varieties of Hibiscus syriacus. Insect pests were investigated on May, July and September in Suwon and Busan, respectively. In addition, change of insect pests on H. syriacus in Seoul was weekly investigated from May to September. As a result, 11 species 116 individuals were observed in Suwon, and 10 species of them were classified as insect pests. Also, 10 species 496 individuals were observed in Busan, and 7 species of them were classified as insect pests. In Seoul, 12 species 181 individuals were observed. Abundance and species richness of insect pests were depend of the study regions but not by H. syriacus varieties. Aphis gossypii was dominant in early May but they were dramatically disappeared since middle May. Other insect pests such as Rehimena surusalis, Haritalodes derogate, and Plautia stali were occurred from July to september.
187.
2019.04 구독 인증기관·개인회원 무료
This study was conducted to investigate the effect of attractant mixture on capture efficacy on Platypus koryoensis, an insect vector of oak wilt disease in Korea. We compared three treatments, such as Citral:EtOH(5:95), EtOH, and no attractant. In order to compare the attractants, Lindgren multi-funnel traps were installed in three oak forests in the Incheon Grand Park. A total of 18 traps was installed, and traps were replaced biweekly. A total of 236, 85, and 25 individuals were collected in Citral:EtOH, EtOH, and control, respectively. Although a mixture of Citral:EtOH seems more effective in attracting P. koryoensis, number of individuals of P. koryoensis caught by attractants was different according to study sites.
188.
2019.04 구독 인증기관·개인회원 무료
Solenopsis geminata has been found in South Korea, suggesting a risk of its invasion has been increased by rapid climate change. This situation requires species distribution modeling to predict possibility of Solenopsis geminata introduction, but information necessary for performing it is very limited. In this study, we developed a map for global distribution of Solenopsis geminata so that the map can be used for future species distribution modeling. Also, as the first step to assess Solenopsis geminata introduction, climatic similarity between its origin (Puerto Rico) and major cities in South Korea was compared. We used ArcMap (version 10.0) for creating the distribution map by obtaining current habitat from public database, and CLIMEX was used to compare climates based on CMI value. The result showed that climates were not similar as indicated by CMI less than 0.52, suggesting the risk of intial introduction is low under the current climatic condition. However, it should be noted that climatic similarity did not consider biological characteristics of Solenopsis geminata and climate change. Thus, the next study will be devoted to climatic suitability simultaneously considers meteorological data, distribution and biological information.
189.
2019.04 구독 인증기관·개인회원 무료
Quarantine Pest Research Facility (QPRF) had been built in Animal and Plant Quarantine Agency for study on exotic pests at 2018. This facility was constructed to prevent effective containment for preventing accidental escape of insects. We tested a developmental and susceptibility of pesticides (Fluxametamide, FLX and Hydramethylnon, HMT) on beet armyworm, Spodoptera exigua for testing containment level of QPRF. S. exigua showed 22.5 days at 28±1℃ and 26.5 days at 25±1℃ with 60±5% RH for entire life cycle. LC50 of FLX and HMT to 5th instar larvae for 24h revealed as lower than 1ppm and 100ppm, respectively. Also, there is no release of adults in QPRF and confirmed by sex pheromone trap during winter season.
190.
2019.04 구독 인증기관·개인회원 무료
The red imported fire ant (Solenopsis invicta) is categorized into the world’s 100 worst invasive alien species by the International Union for Conservation of Nature. Accordingly, the prediction of potential distribution of the red imported fire ant is demanded in order to provide the possibility of dispersion into new sites, and to identify vulnerable regions to be attacked. In general, species distribution model can predict potential distribution of a specific species, but most of them have used air temperature as the most important variable. However, red imported fire ant has life cycle under ground, requiring soil temperature for more reliable prediction of potential habitats. For this reason, this study was to insert soil temperature into CLIMEX, and to evaluate potential distribution of the red imported fire ant.
191.
2019.04 구독 인증기관·개인회원 무료
Japanese pine sawyer, Monochamus alternatus, is the main pest that mediates pine wilt nematode, Bursaphelenchus xylophilus, that causes serious damage to pine forests. In this study, we studied the strategy to control M. alternatus using entomopathogenic fungi. The fungi were collected from soil by an insect-baiting method and two fungal isolates (Metarhizium anisopliae JEF-197 and JEF-279) showed high virulence against M. alternatus. The Metarhizium isolates were evaluated for insecticidal activity against M. alternatus by spray treatment on live pine trees and wintering trees, and the M. anisopliae JEF-197 showed high insecticidal activity. In addition, the interaction of fungi and M. alternatus were analyzed by RNA-seq. This result can contribute to the development of insect control agents using entomopathogenic fungi.
192.
2019.04 구독 인증기관·개인회원 무료
In order to investigate novel insect growth regulators (IGRs) from actinomycetes, 363 isolates of actinomycetes were tested for their IGR activities. Among them, Streptomyces sp. AN120537 showed the highest juvenile hormone antagonist (JHAN) activity and significant insecticidal activities against larvae of Aedes albopictus and Plutella xylostella. In addition, the dead larvae showed morphological deformities such as contraction of body segments or pigmentation of all body parts. Through liquid chromatography and bioassay-guided fractionation, 7 IGR compounds were identified from the crude hexane extract of AN120537. These results suggested that Streptomyces sp. AN120537 could be useful resources for development of eco-friendly insecticidal agents.
193.
2019.04 구독 인증기관·개인회원 무료
Mosquitoes are medically important insect pests that act as vectors of various diseases when they feed on humans. Insect growth regulators (IGRs) could become an effective alternative to control mosquitoes because of their high specificity and relatively low toxicity to environment. Recently, we have developed high-throughput juvenile hormone antagonist (JHAN) screening system based on yeast-two hybrid assay. Actinomycetes have been reported to produce various bioactive compounds including insect growth regulators (IGRs). In this study, culture filtrates and mycelia extracts of 2,875 actinomycetes isolates screened for their IGR activities and mosquitocidal activities. Among 75 culture filtrates and 17 mycelia extracts with JHAN activities, 19 culture filtrate and 3 mycelia extracts showed high level of mosquitocidal activities against 3rd instar larvae of Aedes albopictus. These results suggested that secondary metabolites of actinomycetes could be used as environmentally benign mosquitocidal agents.
194.
2019.04 구독 인증기관·개인회원 무료
The global biopesticide market was estimated to become about 4% of the total crop protection market in 2015, mainly due to variability of their efficacy, narrow spectrum or difficulties in long-term storage. Therefore, many people focus on overcoming these issues as a big trend. Suggested solutions include the investigation of synergy between microorganisms, the use of genetic engineering, improving the pesticide life shelf, etc. As a result, biopesticides market has grown by more than 17% over the last decade. In this context and aiming to develop new entomopathogenic fungi–based pest management tools, we constructed a fungal library by isolating insect pathogenic fungi from soil. A total of 581 isolates belonging to 35 species were isolated and characterized. Beauveria bassiana was the most abundant, representing 38.55% of the total strains, followed by Metharizium anisopliae (22.55%) and bubillosa (8.6). …% of the total isolates were highly virulent against Tenebrio molitor killing most of the treated insects in 2 to 3 days.
195.
2019.04 구독 인증기관·개인회원 무료
The Ryanodine Receptor in wild type Drosophila melanogaster has an amino acid substitution which is known to cause Chlorantraniliprole resistance in Pluteall xylostella. Even though we have reported that two Chlorantraniliprole resistant Drosophila strains have elevated total esterase activities, our report does not fully explain the significantly increased resistant ratios in two Chlorantraniliprole resistant Drosophila strains. Thus, we further analyzed alteration of reactive oxygen species and mitochondria activities in two Chlorantraniliprole resistant Drosophila strains. Our result suggested that Chlorantraniliprole resistance development in Drosophila requires alteration of various signal transduction pathways.
196.
2019.04 구독 인증기관·개인회원 무료
The effect of fumigation on the phosphine-susceptible and -resistant strains in the T. castaneum was evaluated using phosphine, ethyl formate, and combination with phosphine and ethyl formate (phosphine+ethyl formate). The Lethal Concentration Time (LCT)50 analysis of susceptible strains, late larva showed that phosphine (0.13 mg·h / L), ethyl formate (80.91 mg·h / L), and phosphine + ethyl formate (19.36 mg·h / L). The LCT50 of adult was 0.05 mg·h / L, 68.58 mg·h / L and 17.84 mg·h / L when treated with phosphine, ethyl formate, and phosphine + ethyl formate. The LCT50 of resistant strains, late larva was found to 33.32 mg·h / L of phosphine, 113.46 mg·h / L of ethyl formate and 129.85 mg·h / L of phosphine + ethyl formate, and the LCT50 of adult was 55.71 mg·h / L of phosphine, 85.39 mg·h / L, phosphine + ethyl formate 85.83 mg·h / L. The treatment of three fumigants (phosphine, ehtyl formate, and phosphine+ethyl formate) showed the possibility of controlling against T. castaneum of phosphine-susceptible and –resistant strains.
197.
2019.04 구독 인증기관·개인회원 무료
Melon thrips, Thrips palmi Karny (Thysanoptera: Thripidae) is a worldwide polyphagous pest. The management of this pest have mainly relied on chemical agents. However, the overuse is harmful to the environment and results in insects resistance. Therefore, alternative eco-friendly control methods that have different mode of action, such as biological control, are necessary to overcome the current issue. In this study, we isolated entomopathogenic fungi from Korean soil, and characterized them via morphological and molecular techniques and pathogenicity assay against Tenebrio molitor larvae. The isolated fungi were screened for virulence against T. palmi under laboratory conditions and the results were used to establish a thrips-pathogenic fungal library. The highly virulent isolates were selected and further characterized for optimum culture conditions and application as biopesticide in the field.
198.
2019.04 구독 인증기관·개인회원 무료
본 연구에서는 주요 발생지인 구례 지역의 산수유, 때죽나무를 중심으로 2018년 8월에서 9월 사이에 갈색날개매미충 월동 난괴를 채집하여 날개매미충알벌(phanuromyia spp.)의 온도별(15℃, 20℃, 25℃, 30℃) 발육기간, 수명 및 우화 후 15일간의 총 산란수를 조사하였다. 날개매미충알벌의 발육기간은 25℃에서 알 2.6일, 유충 5.4일, 번데기 15.0일, 30℃에서 알 1.9일, 유충 7.6일, 번데기 13.7일이 소요되었다. 날개매미충알벌 성충의 온도별 수명은 15℃에서 암컷 77.4일, 수컷 66.6일, 20℃에서 암컷 49.0일, 수컷 44.0일, 25℃에서 암컷 27.6일 수컷 28.4일, 30℃에서 암컷 18.0일, 수컷 14.0일로 암수 모두 사육온도가 높아질수록 수명이 짧아졌으며 15℃와 30℃에서만 암수 수명의 차이가 있었다. 또한 온도별 총 산란 수는 15℃를 제외한 나머지 세 가지 사육온도에서 산란 수 차이는 없었으나 25℃에서 평균 23.2개로 가장 높았다.
199.
2019.04 구독 인증기관·개인회원 무료
Spotted wing drosophila, Drosophila suzukii, causes damage on several fruits include strawberry in Korea. D. suzukii lays egg inside fruit surface, and this behavior makes D. suzukii hard to detect, so many countries designated D. suzukii as a quarantine pest. Recently, Australian government demand methyl bromide fumigation on Korean export strawberry to prevent D. suzukii invasion. In this study, we tested methyl bromide to control D. suzukii, and also tested separate and concurrent treatments of methyl bromide to evaluate phytotoxicity on strawberry. When 40 g/m3 of methyl bromide was treated for 3 hours, all stages of D. suzukii were completely controlled. When treated separate and concurrent with cold temperature treatments of methyl bromide, there’s no specific phytotoxicity on strawberry. This result indicates that separate and concurrent treatments of methyl bromide do not cause phytotoxic effect on strawberry and methyl bromide is effective to control D. suzukii.
200.
2019.04 구독 인증기관·개인회원 무료
Monochamus alternatus which is major vector for Bursaphelenchus xylophilus is main pest for pine trees. Moreover, their larvae mainly feed on the phloem of the host during the growth period, and this process makes host debilitate. The reason why the epidemiological investigation of that damage caused by Monochamus alternatus is difficult is that the adult and the larva remains very rarely in the host. Even if the larvae remain in the host, it is not easy to distinguish them from other cerambycidae coleopterans without expertise. For the above reason, we introduce the method of field epidemiological investigation for Monochamus alternatus using the remaining larva frass in the host with Recombinase Polymerase Amplification (RPA) method.