간행물
한국응용곤충학회 학술대회논문집
- 발행기관 한국응용곤충학회
- 자료유형 학술대회
- 간기 반년간
- 수록기간 2000 ~ 2024
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 495DDC 595
권호리스트/논문검색
한국응용곤충학회 50주년 기념 국제 심포지엄 (2011년 5월) 233건
41.
2011.05
구독 인증기관·개인회원 무료
The two-spotted spider mite, Tetranychus urticae Koch, is an economically important pest of crops of plant grown in the field or greenhouse worldwide. It has recently become a serious problem because of the continuous use of acaricides resulting in resistance among spider mite population. Thus, there is a need to find alternative control measures to suppress spider mite populations. In this study, we report the screening result of pathogenic fungi for the control of spider mite. Initial screenings were performed using 352 isolates of putative pathogenic fungi from Korea soils. As results, 11 strains of acaropathogenic fungi were isolated from 8 cadavers of spider mite supporting fungal conidiation. These isolated were identified as four isolates of Beauveria bassiana (6, 2R-3-3-1, 2R-4-5, 2R-4-7), two isolates of Metarhizium anisopliae (4-2, 2-2), one isolate of Clonostachys rosea 5-2, one isolate of Lecanicillium attenuatum 4-1, one isolate of Pochonia suchlasporia 2R-3-1, one isolate of Aspergillus flavus 7 and one isolate of Isaria lilacinus 2R-4-6 by microscopic examination and genetic sequencing of the ITS region. Based on the screening results, eleven isolates were tested for their virulence against adult spider mites. All fungal isolates were pathogenic to spider mite but mortality varied with isolates. These acaropathogenic fungi may be useful to develop eco-friendly acaricide to control two-spotted spider mite.
42.
2011.05
구독 인증기관·개인회원 무료
Methyl bromide (MB)는 대상범위가 넓고 속효성을 가지고 있어 세계적으로 널 리 사용되어 왔으나 오존층 파괴물질로 등록이 되어 대체 훈증제 개발이 시급한 상황이다. MB 대체 훈증제로 ethyl formate와 Eco2fume (PH3 2% + CO2 98%, PH3)의 월동형 점박이응애에 대한 살충효과와 단감에 대한 약해를 조사하였다. 월 동형 점박이응애는 경남 거창군의 사과 과수원에서 채집하였다. 훈증농도는 EF는 5.8~34.2 mg/L, PH3는 0.1~1.5 mg/L이었으며 훈증시간은 EF는 6 시간, PH3는 24 시간으로 두 약제 모두 5±1 ℃에서 처리하였다. EF의 경우 LD50과 LD99값은 각각 66.3, 148 mg h/L 이었다. PH3 경우 LD50과 LD99값은 각각 6.5, 57.8 mg h/L이었 다. 100% 사충률을 얻기기 위해서 EF는 148 mg h/L 이상, PH3는 57.8 mg h/L 이 상의 CT값이 필요하였다. EF와 PH3 훈증으로 인한 단감의 약해(경도, 당도, 부패 율 등)는 없었다.
43.
2011.05
구독 인증기관·개인회원 무료
파리목 곤충의 청각기관은 더듬이의 존스턴 기관으로 알려져 있어, 소리에 대한 아메리카잎굴파리 더듬이의 반응을 EAG를 이용하여 조사하였다. 순음(사인파)과 아메리카잎굴파리 및 다른 3종의 구애노래를 절제된 아메리카잎굴파리 머리에 노 출시키고 EAG를 통하여 반응을 관찰하였다. 순음의 경우 20 Hz의 저주파에서만 EAG 반응이 관찰되었다. 구애노래의 경우 검정발노랑굴파리(Cryptonevra inquilina) 와 일본의 Lipara japonica 구애노래에 대한 반응이 나타났으나 아메리카잎굴파 리 수컷의 구애노래에 대한 암컷의 EAG 반응은 나타나지 않았다. 조사된 음파에 대한 반응행동을 관찰하기 위해 2차원 궤적 추적을 추가적으로 실행하였다. 기록 된 궤적은 x축 궤적의 평균값을 비교하여 소리에 대한 유인효과를 조사하였고, 궤 적의 정보 엔트로피를 이용하여 활동성 차이를 분석하였다. x축 평균값은 소리에 대한 차이가 없어, 소리에 대한 아메리카잎굴파리의 유인효과는 관찰하지 못 하였 다. 처녀 수컷과 암컷, 교미한 수컷에서는 소리를 노출시켰을 때 정보 엔트로피 값 이 감소하여 활동성이 차이가 남을 알 수 있었으나 교미한 암컷에서는 활동성의 차이가 없었다.
44.
2011.05
구독 인증기관·개인회원 무료
The American house dust mite, Dermatophagoides farinae Hughes, is the most important factor of allergic diseases, such as atopic dermatitis, rhinitis, and asthma. The protein-denaturing activity of nerolidol (1), chrysin (2), and spathulenol (3) identified in the Brazilian propolis against D. farinae was evaluated using SDS-PAGE and dot-blot immunoassay. Results were compared with those of the currently available dust mite protein-denaturing agent tannic acid. SDS-PAGE showed that application of test compounds and tannic acid (100 μg each) caused complete disappearance of D. farinae protein bands. In a dot-blot immunoassay, test compounds and tannic acid (100 μg each) strongly inhibited the IgE-binding reactivity to D. farinae protein of a highly mite-sensitive asthmatic patient. The Brazilian propolis constituents described merit further study as potential dust mite-allergen denaturants for protection from humans from various diseases caused by house dust mites.
45.
2011.05
구독 인증기관·개인회원 무료
Bursaphelenchus xylophilus is known to be a major pathogen of the pine wilt disease (PWD). The pathogenecity of PWD is considered to be related to cell wall-degrading enzymes such as endoglucanases, expansins and pectate lyases (PELs). Two PELs, Bx-PEL1 and Bx-PEL2 are known to be expressed in B. xylophilus and regarded as a putative pathogenic factor. Recently, we developed stage-specific expressed tag library of B. xylophilus and identified a novel Bx-PEL3. We cloned Bx-PEL3 gene with RT-PCR, which showed high similarity to previously reported Bx-PELs. Phylogenetic analysis revealed that PEL3 was much closer to Bx-PELs than any other PELs. PEL3 has a conserved intron site as found in other Bx-PELs in the genomic DNA. Quantitative real-time PCR analysis revealed that Bx-PEL1 and Bx-PEL2 were more predominant in B. xylophilus than the Bx-PEL3. Recombinant Bx-PEL3 showed the activity for polgalacturonic acid and its physical conditions such as PH and Ca2+concentration for optimized activity were 9.0 and 0.5 mM, respectively. The localization of PEL3 transcript is the anterior body of B. xylophilus, near the esophageal gland. Taken together, these results suggest that a novel PEL3 gene is biochemically functional and can play a role as a putative pathogenic factor like other PELs.
46.
2011.05
구독 인증기관·개인회원 무료
Sung Min Bae, Bit Na Rae Yun, Jun Beom Lee, Hee Jung Kim, Tae Young Shin, Jae Bang Choi, Yeon Ho Je, Byung Rae Jin, Soo Dong Woo
Polyhedrin is the major component of the nuclear viral occlusions produced during replication of the baculovirus Autographa californica multicapsid nuclear polyhedrosis virus (AcMNPV). To enhance the expression level of baculovirus vector system, we constructed several fusion vectors using various fragments of the polyhedrin. The polyhedrin fragments were genetically fused to the enhanced green fluorescent protein (eGFP) under the control of polyhedrin promoter, and their expressions were analyzed in Sf21 insect cells. Expression of the fusion protein was identified by SDS-PAGE and Western blot analysis using anti-GFP and anti-Polyhedrin. The expression level of eGFP was markedly increased by the fusion of partial polyhedrin. Also, the fluorescence intensity of fusion proteins was higher than that of non-fusion protein. Confocal laser scanning microscopy demonstrated that fusion proteins were localized to the cytosol or nucleus of insect cells. In additional, the glycoprotein E2 (gE2) of classical swine fever virus (CSFV) expressed by the these vectors was dramatically increased and its immunogenicity was proofed using experimental animal guinea pigs that were immunized with the partial polyhedrin containing gE2. This study provides a new option for the higher expression of useful foreign recombinant protein by using the partial polyhedrin in BEVS.
47.
2011.05
구독 인증기관·개인회원 무료
The Wolbachia bacterium is known to induce reproductive anomalies in various insect taxa such as cytoplasmic incompatibility, feminization, male killing and parthenogenesis. It is hypothesized that the degree of reproductive anomalies is dependent on the bacterial infection density. In this study, we attempted to test the hypothesis using the tiny egg wasp, Trichogramma kaykai that has served as the model system of parthenogenesis where an unfertilized egg develops into a female due to the bacterial infection. So far this is only found in haplodiploid organisms. The results show that 1) as mothers aged, they started producing male offspring, 2) the sex ratio was negatively correlated with the bacterial infection density, 3) female offspring were more than six times heavily infected with Wolbachia than male offspring in the species. In conclusion, female offspring production, parthenogenesis, is as a function of the Wolbachia bacterial density in this species.
48.
2011.05
구독 인증기관·개인회원 무료
The four genetically distinct isolates have been identified previously from Bombyx mori nucleopolyhedroviruses (BmNPVs) isolated in Korea. To further understand the complex of viruses infecting Bombyx mori, the genome of BmNPV-K1 and K4 strains was completely sequenced and analyzed in comparison with the genome of other sequenced baculoviruses including previously reported BmNPV. BmNPV-K1 consisted of 127,542 bp and 133 open reading frames (ORFs) of 150 nucleotides or longer with minimal overlap have been identified. In contrast, BmNPV-K4 consisted of 128,615 bp and 134 open reading frames (ORFs). Although gene arrangement is virtually identical, the genome of BmNPV-K4 is 1,073 bp longer than BmNPV-K1. This was related to the more existence of bro genes in BmNPV-K4. To investigate the relationship between BmNPV-K1 and K4, phylogenetic analysis with each member of the paired ORFs was performed. The sequence data suggest that BmNPVK1 and BmNPV-K4 are closely related but have diverged and evolved into two separate strains. This was study to identify highly related but separately evolving viruses in the same insect host and geographic location. We are currently comparing the differences of these BmNPV genomes to elucidate characteristics of each virus.
49.
2011.05
구독 인증기관·개인회원 무료
Light brown apple moth, Epiphyas postvittana, is a significant horticultural pest native to Australia, and currently with a limited global distribution. However it can tolerate very heterogeneous climatic and vegetation conditions and has recently invaded California with considerable consequences for US international and domestic trade. By comparing the climatic conditions of its native (Australia) and long-established (New Zealand) ranges to the rest of the world using CLIMEX, it was suggested that E. postvittana has potential to establish mainly in countries in Central and South America, southern Africa, west Europe and South-east Asia. However, the predicted global distribution of E. postvittana using a new multiple-species-distribution model system suggested that there are additional climatically suitable areas around the world where this species could potentially survive and establish. Our study provides basic but important information for further assessment of the establishment capacity of this species in new habitats, wihch will provide the knowledge required to make science-based decisions in biosecurity.
50.
2011.05
구독 인증기관·개인회원 무료
경남의 대표적 습지인 주남저수지 중 주남저수지(3개지점), 동판저수지(3개지 점), 산남저수지(3개지점) 및 인공연못(전체)을 대상으로 2010년 5월부터 10월까 지 육상곤충의 다양성과 군집특성을 조사하였다. 총 12목 77과 273종이 조사되었 는데, 나비목이 61종, 딱정벌레목이 54종, 노린재목이 34종이었다. 개체수는 딱정 벌레목이 1450개체로 가장 많았고 그 다음이 잠자리목 1274개체, 나비목 878개체 순이었다. 조사지점별로는 주남저수지 169종, 동판저수지 185종, 산남저수지 154 종이 조사되어 비슷한 양상을 보였으나 인공연못에서는 86종이 조사되었다. 전지 점을 종합해 볼 때 동판저수지와 산남저수지에서 우점종인 일본잎벌레가 우점종 이었는데, 이는 수생식물인 마름이 기주이기 때문에 일본잎벌레가 습지환경에 잘 적응했기 때문으로 생각된다. 곤충 군집 분석 결과 우점도지수(DI)는 인공연못이 0.25로 가장 높고 주남저수지가 0.08로 가장 낮았다. 다양도지수(H')는 주남저수 지가 4.48, 동판저수지가 4.44, 산남저수지가 4.28, 인공연못이 3.87로 모두 높았 다. 한편 지점별 우점도지수, 다양도지수, 균등도는 월별로 큰 차이를 보이지 않았 는데 이는 주남저수지가 계절적으로도 곤충이 지속적으로 출현하는 안정된 곤충 상을 갖고 있음을 의미한다고 할 수 있다.
51.
2011.05
구독 인증기관·개인회원 무료
The study was conducted to investigate the spring emergence pattern of G. molesta and to forecast the emerging time of overwintering G. molesta on tree fruit orchards. G. molesta is one of major insect pests on fruit trees in Korea. The host range of G. molesta includes many economically important tree fruit plants such as apple, pear, peach and plum. The overwintering G. molesta emerge from late March as an adult lay eggs on the shoot of peach or fruits of apple, plum and peach. Therefore, it is important to understand the biofix and to forecast the emerging peak period of overwintering G. molesta for establishing the pest management strategy. The pheromone trap of G. molesta has been utilized to monitor the population density in apple orchard. The commercial stick trap (GreenAgroTech) and lure (Z8-12:AC, E8-12:Ac, Z8-12:OH, 95:5:1) was set to monitor the population density of G. molesta on each place (56 different fruit orchards). The record of temperature was received from meteorological center close to monitoring orchards. The parameters for forecasting the emerging time and peak period of overwintering G. molesta were calculated from the results of Yang et al (1997 and 2001). Although the estimated biofix of G. molesta was not fitted well, the peak period of overwintering G. molesta was explained by linear regression model. The spring emergence pattern of G. molesta was presented differently related to host plant and geographical location. The peak period of G. molesta at the same mornitoring county was presented differently according to host plant. The synchronization between host plant and G. molesta may be studied to figure out the spring emerging time of overwintering G. molesta.
52.
2011.05
구독 인증기관·개인회원 무료
오늘날 통계적 사고와 통계적 기법의 활용은 학문분야에 있어 보편화되고 있는 추세이다. 또한, 국내 학술지가 국제 학술지로 격상되고 있는 현재, 논문에서의 통 계 기법 사용의 적정성을 점검해 볼 필요가 있을 것이다. 본 논문에서는 응용곤충 학 관련 학술논문집간 통계적 기법의 사용빈도를 비교해봄으로써 국내논문의 통 계 기법 사용의 현재 위치에 대해 고찰하고, 국내논문이 나아갈 방향에 대해 모색 해보았다. 본 연구에는 통계 빈도에 대한 조사를 비교하는 데에는 2005년에서 2009년까지 5년간 국내·외 학술논문에 기재된 논문 대상으로 Fisher's exact test를 사용하였다. 우선 국내와 일본 학술논문집간 비교 결과, 2007년까지는 통계적 처 리기법의 사용빈도에서 유의한 차이를 보였으나 그 이후에는 유의한 차이가 나타 나지 않았다. 국내와 네덜란드의 학술논문집간 비교에서는 2009년을 제외하고는 통계적 처리기법의 사용빈도에서 모두 차이가 있는 것으로 나왔다. 참고적으로 일 본과 네덜란드의 비교에서는 통계적 처리기법의 사용빈도에서 차이가 없었다. 이 러한 결과를 토대로, 국내 학술논문집에서의 통계적 기법의 사용빈도가 점차 국외 학술논문집들과 비슷해지는 과정에 있다는 것을 알 수 있었다. 따라서 논문에서의 통계적 기법을 사용하는 것에 더욱 신중하고 노력을 기울일 필요가 있겠다.
53.
2011.05
구독 인증기관·개인회원 무료
The α-proteobacterium Wolbachia pipientis infects a wide range of arthropods and filarial nematodes. Wolbachia is maternally inherited and is known to induce reproductive anomalies such as cytoplasmic incompatibility, feminization, male killing and parthenogenesis induction (PI). Trichogramma kaykai is a tiny wasp that parasitizes on lepidopteran eggs. When a female of the wasp is infected with PI-Wolbachia, the female produces female offspring via gamete duplication without the aid of sperm. As she ages, however, the fraction of male offspring increases. In this study, we investigated the effect of host genetic background on the expression of sex ratio between isofemale lines. Virgin females of six isofemale lines were allowed to lay eggs individually for 10 days. There was the positive relationship between female age and the offspring sex ratio. Furthermore, the sex ratio was significantly different among isofemale lines, implying that the host genetic background had an effect on the sex ratio. Based on the results, evolution of symbiosis in terms of sex ratio and future experimental design are discussed.
54.
2011.05
구독 인증기관·개인회원 무료
Species of root knot nematodes, Meloidogyne spp. were identified through the PCR amplification and sequence analysis of mitochondrial DNA (mtDNA) region between COII and lrRNA genes. Root knot nematodes were extracted from 5 plant samples [kiwi (Japan), rhododendron (Japan), cornus (Japan), ficus (China) and jasmine (Vietnam)] that were collected from Plant Quarantine Station in Korea. At first, species was identified using morphological characters, such as the length and shape of stylet, the tail length and the perineal patterns. Secondary, single individuals of either female and juvenile collected from plant tissues were used for PCR and further sequencing analysis. The results showed that cornus, jasmine and ficus plants were infected by M. incognita, and kiwi and rhododendron plants were infected by M. interolobii.
55.
2011.05
구독 인증기관·개인회원 무료
The tribe Phylini (Miridae: Phylinae) is revised in the Korean Peninsula. A total of 65 species in 20 genera are confirmed to exist in this territory. Five genera and 20 species are recognized for the first time in Korea, including a new genus and 6 new species: Gen. nov. sp. nov.1; Psallus sp. nov.1, P. sp. nov.2, and P. sp. nov.3; and Moissonia sp. nov.1 and M. sp. nov.2. Herein, checklist for the Korean Peninsula is reconstructed with its geographical distribution, and short discussion.
56.
2011.05
구독 인증기관·개인회원 무료
Tetranychid mites are one of the most diverse group including at least 1,200 species in the world. Species identification is difficult due to the small size and similar morphology within the group. We collected 17 species of spider mites from various host plants in different regions of Korea and determined species identity by the comparison of morphological characters and nucleotide sequences of internal transcribed spacer 2 (ITS2) and the cytochrome oxidase subunit I (COI). In addition, we report three new species that were firstly identified in Korea. Amphitetranychus quercivorus (Ehara and Gotoh) was collected from Mongolian oak plant in Daegu, Schizotetranychus miscanthi Saito was from the common reed plant in Ulleungdo, and S. cercidiphylli Ehara was from Bamboo plant in Jeju. Morphological identification of three species were similar with those of Japanese samples, but the ITS2 and COI sequences of A. quercivorus and the COI sequence of S. miscanthi were different with Japanese species at the rates of 1/419, 2/331 and 3/332 nucleotides, repsectively. S. cercidiphylli can be identified by the aedeagus shape of males but we firstly sequenced ITS2 and COI of this species. Our results can be used for the identification of spider mites which are important in plant quarantine.
57.
2011.05
구독 인증기관·개인회원 무료
Two complete mitochondrial genomes of the tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuoidea) and the rice leaf roller, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae), were sequenced. Each 15,388 bp and 15,368 bp-long genome contained both the lepidopteran specific gene arrangement that differ from the most common arrangement of insects by the movement of tRNAMet to a position 5’-upstream of tRNAIle. Neither of the species have typical COI start codon. Instead, the CGA (arginine) sequence that is commonly present in other lepidopterans was also found both in S. litura and C. medinalis. The evolutionary rates among 13 protein-coding genes (PCGs) in Lepidoptera showed ATP8 the highest, whereas COI the lowest. The high A+T-content, which is characteristic of mitochondrial genome was well reflected in the two lepidopteran mitochondrial genomes: higher frequency of A/T-rich codons, severe A/T bias in 3rd codon position, and extremely high A/T content in the A+T-rich region. Because insect mitochondrial genomes harbor biased nucleotide and resultantly biased amino acid sequences, phylogenetic inference is often misled by them. Although each recoded and unrecoded datasets for nucleotide sequences and amino acid sequences of PCGs provided overall identical topology, regardless of recoded scheme, each nucleotide and amino acid dataset provided difference in the status of Macrolepidoptera, providing a monophyletic group by amino acid dataset, whereas non-monophyletic group by nucleotide dataset.
58.
2011.05
구독 인증기관·개인회원 무료
The phylogenetic relationships among the Nymphalidae (Lepidoptera: Papilionoidea) have been controversial. The present study sequenced approximately 1,099 bp from cytochrome oxidase subunit I (COI), 1,336 ~ 1,551 bp from 16S ribosomal RNA (16S rRNA), and 1,066 bp from elongation factor-1 alpha (EF-1α) in 80 species belonging to seven subfamilies (Linmenitidinae, Heliconiinae, Nymphalinae, Apaturinae, Libytheinae, Satyrinae, and Danainae) of Nymphalidae, along with those of six lycaenid species as outgroups. The average base compositions for the three genes (COI, 16S rRNA, and EF-1α) are as follows: A (30.6%, 38.8%, and 25.8%), G (14.7, 5.2%, and 23.6%), T (39.8%, 45.2%, and 23.4%), and C (14.9%, 10.8%, and 27.3%). This result shows the A/T bias in the mitochondrial genes, but not for the nuclear EF-1α. Between the two mitochondrial genes, the 16S rRNA gene evidenced a significantly higher A/T content than was detected in the COI gene. These sequences were subjected to phylogenetic reconstruction via Bayesian Inference (BI) and Maximum Likelihood (ML) algorithms. Both analyses concordantly supported the subfamilial relationships of (((((Linmenitidinae + Heliconiinae) + (Nymphalinae + Apaturinae)) + Libytheinae) + Satyrinae) + Danainae), along with highly supported monophyletics of tribes within subfamilies. This result is largely consistent with a previous study performed with a large sequence information and morphological characters, except for the position of Libytheinae, which was suggested to be the basal lineage of Nymphalidae.
59.
2011.05
구독 인증기관·개인회원 무료
In traditional taxonomy on the family Cantharidae, color pattern of the body and the structure of the male genitalia have been often used as diagnostic characters in identification of the specific level. However, these characters caused the difficulty in identifying the female in case a species was described only by male specimens or has the several color types among individuals. In this study, we attempted to evaluate the species reality of Asiopodabrus fragiliformis which was often difficult to be identified due to individual variation in color pattern and lack of information of female, through searching for new morphological diagnostic characters as well as DNA barcoding analysis, including their closely relative species from Russia and Japan. The results showed that A. fragiliformis was represented as three clusters strongly supported by high value of boots trap (>99%) and over 3% branch length. The pairwise distances between species of Asiopodabrus were detected larger, ranged from 3.4–9.5%, than the intragroup distance ranged from 0–2.9% indicating presence of a barcoding gap. And then, the three clusters were respectively determined as A. fragiliformis, A. kurvatovi and a new species through the analysis of morphology and COI gene. Therefore, we suggest that the species delineation on polymorphic species and the female specimens of closely resembling species would be more exactly and effectively determined if DNA barcoding and the traditional taxonomy are used as complementary methods for identification.
60.
2011.05
구독 인증기관·개인회원 무료
범부전나비(Rapala caerulea)와 울릉범부전나비(Rapala arata)는 형태적 유사 성이 높은 종들로 일반적으로 날개 윗면의 색으로 구분되어 왔다. 또한, 지리적 분 포 특성으로 볼 때 범부전나비는 중국 중부, 북동부에서 한반도(울릉도, 제주도 제 외)까지 서식하는 반면, 울릉범부전나비는 극동러시아, 중국 동북부, 한국의 울릉 도와 제주도, 일본까지 출현한다. 두 종의 분포가 겹치는 한국에서는 날개의 체색 만으로는 두 종의 진단이 모호하므로 뒷날개 밑면 후연의 점무늬로 구분한다. 즉, 2개의 흑점무늬를 갖는 종은 범부전나비, 4개의 흑점무늬를 띤 종은 울릉범부전나 비로 구분하고 있다. 하지만, 두 종 각각의 일부 개체에서는 흑점무늬의 배열에서 중간형이 지속적으로 출현하는 것이 확인되어왔다. 이처럼 두 종간의 형태적 진단 형질의 부분적 중복으로 인한 모호성을 극복하기 위하여 COI유전자를 이용하여 두 종의 지역집단(한국 3개 집단, 일본 1개 집단) 26개체에 대한 DNA 바코딩 분 석을 실시하였다. 하지만 그 결과로 분석된 26개체에 대한 COI 서열 차이는 0.0-0.3%로 극히 작았을 뿐 이었다. 특히, 0.2-0.3%의 서열변이를 나타낸 범부전 나비의 경상북도 울진산 2개체의 경우도 1개의 염기만이 차이가 있었을 뿐 나머지 서열은 모두 똑같은 것으로 나타났다. 따라서 2종 간에 barcoding gap을 형성할 만 큼의 차이는 전혀 없었으며, 두 종은 동일종의 집단으로서 집단 간 변이도 크지 않 았던 것으로 해석되었다. 이에 따라서 향후 형태형질의 추가적 분석을 통해 종합 분류를 수행하면서 동물이명 처리가 필요할 것으로 판단되었다.
