To investigate the differential expression of genes by 635nm LEDs irradiation in arachidonic acid-treated human gingival fibroblasts, cDNA microarray was carried out. Human gingival fibroblasts were primary cultured and arachidonic acid was treated to induce inflammation. 635nm of wave length was used for LEDs irradiation. The experimental group was categorized into four group ; control, only LEDs irradiation group, only arachidonic acid-treated group and arachidonic acid-treated with LEDs irradiation group. The expression of 8,078 genes were increased and the expression of 7,103 genes were decreased in only LEDs irradiation group. For arachidonic acid-treated with LEDs irradiation group, the expression of 6,815 genes were increased, while the expression of 8,031 genes were decreased comparing with only arachidonic acid-treated group. IL-13alpha2 receptor was the most expressed gene in LEDs irradiation group comparing with control, followed by MMP3. Genes which the most down regulated was BIRC3 in LEDs irradiation group. PLAB genes was the most up-regulated in arachidonic acid treated with LEDs irradation group, followed by ranked RARRES1. Considering the classification by cell function, genes associated with signal transduction were the most affected by LEDs irradiation, followed by the genes associated with nucleoside, nucleotide and nucleic acid metabolism. In arachidonic acid treated with LEDs irradiation, genes associated with signal transduction and protein metabolism were affected. Taken together, LEDs irradiation could affect various biological process and could identify many genes related to LEDs irradiation, which could be used for clinical application.

Bacillus thuringiensis with selected high toxicities against tobacco cutworm, Spodoptera litura were isolated from domestic soils. When being observed under a phase-contrast microscope, the insecticidal crystal proteins were showed a bipyramidal crystal types. New CAB 109 isolate was identified to B. thuringiensis subsp. aizawai in the H serotype. As a results of insecticidal activities between CAB 109 isolate and 3 existing ready-made products against 3rd larva of S. litura, CAB 109 isolate showed 100% mortality with spore concentration (1.3×10⁷ cfu/㎖). It was a very high insecticidal activity compared with a existing ready-made B. t. products. LD₅₀ values of CAB 109 isolate was 9.78×10⁵, 6.87×10⁶ and 1.83×10⁷ cfu/㎖ spore concentration against 2nd, 3rd and 4th larva of S. litura, respectively. Unlike Plutella xyfostella, S. litura was slowly died after application up to 7 days. The weight of S. litura larva applied with CAB 109 isolate were 6-7 times less than controlled group. Even though it didn"t die, it did not grow into next larva. The result observed with scanning electron microscope was that CAB 109 isolate of B. t. aizawai formed a typical bipyramidal crystal protein type. Otherwise, when CAB 109 isolate was examined with SDS-PAGE and with trypsin, there was no difference between CAB 109 strain and ready-made products of B. thuringiensis.

Many kinds of environment friendly agricultural materials (EFAMs) were used for the plant protection, furthermore they support the growth of crops in the greenhouses and the kindly environment friendly farming. Natural enemies might be used for control of insect pest with EFAMs at the same space and time. For testing the toxicity of EFAMs against to natural enemies, 10 EFAMs were selected and tested in small greenhouses and farm"s fields. In case of Harmonia axyridis larva, there was no predacious activity against cotton aphids in the block with EFAMMo L, EFAMPE D, EFAMME G and EFAMAE A, otherwise, aphid population was rapidly decreased in the control block without EFAMs. Both of Trialeurodes vaporariorum and Encarsia formosa population were slightly decreased in the block with EFAMs. There were significantly decreased in the block with IEFAM C, FEFAM D, EFAMSM Band EFAMPE D compared with control area. Especially, there was significantly maintained a very low population, both T. vaporariorum and E. formosa in the block with EFAMMo C. The parasitized ratio of Aphidius colemani is also very low in the block with EFAMs compared with control area. In the block with IEFAM C, FEFAM D, EFAMPE D and EFAMMo L, there were significantly different with control area in the number of mummies. Otherwise, in case of Diglyphus isaea and Liriomyza trifolii, there was no significantly different between blocks with and without EFAMs. In the block with EFAMMo C, Tetranychus urticae and Phytoseiulus persimilis populations were significantly maintained a very low level. However, the population of T. urticae was increased, but P. persimilis was decreased in the block with EFAMMo L.

Methanol extracts of 35 plant species in 20 families were tested for their insecticidal activities and repellent effects against Nilaparvata lugens female adult by topical application and spray methods. The insecticidal activities and repellent effects of various plant species and parts were different. The methanol extracts from stem of Garcinia xanthochymus, Senecio scandens and Phytolacca americana, seedcoat of Ginkgo biloba, and leaf + stem of Ailanthus altissima and Catalpa ovata showed potent insecticidal activities against N. lugens. Specially, the G. biloba extract exhibited higher than 90% mortality against N. lugens at a concentration of 4,000 ppm. Repellent effects of plant extracts obtained from whole plant of Daucus carota, fruit of Semecarpus anacardium, leaf + stem of C. ovata and Wisteria sinensis were active, and potent. Also, the plant extracts that are potent in insecticidal activity not necessarily have high repellent activity showing no significant corelation between the two activities.

For the development of friendly environmental control of the beet armyworm, Spodoptera exigua that is too hard to control in the field, 25 insecticides were chosen from 58 registered to the beet armyworm, and bioassayed. There are 12 insecticides with neurotoxical activities, 10 with insect growth regulators and 3 Bacillus thuringiensis products. Among 12 insecticides with neurotoxical activities, mortality of S. exigua was 100% with emamectin benzoate (EC) and indoxacarb (WP) within 3 and 5 days after application, respectively. Otherwise, WG and SC of indoxacarb, Indoxacarb + etofenprox (WP) and pyridalyl (EW) were showed up to 91%. Methoxyfenozide + spinosad (SC) was better than any other insect growth regulator as 100% mortality within 3 days after application. And methoxyfenozide (WP), tebufenozide (WP) and methoxyfenozide (SC) were 92% by 5 days. However, 3 kinds of B. thuringiensis products were showed under 35% mortality within 5 days from first spray.

환경이 조절되는 폐쇄형 식물생장시스템에서 플러그 셀 크기(105공, 162공, 288공)와 일장(8/16, 12/12, 16/8hr, 낮/밤) 처리를 각각 달리하여 20일간 생산된 감자 플러그 묘 '수미'와 대지'의 생육과 수량에 미치는 영향을 알아보고자 실험을 수행하였다. 정식 7주째 '수미'의 플러그 셀 크기와 일장처리는 생육과 상대생장률에 차이를 주었다. '수미'의 주당 괴경 중은 셀 크기와 일장이 증가함에 따라 증가하였다. '대지'의 괴경 중은 105공 셀 크기에서 높았으며, 일장 처리에 차이를 보였다. 90일 재배 후, '수미'의 괴경 중(258.9~471.9g/주)은 105공, 162공 처리와 16/8 hr 일장 처리에서 괴경 중이 높았다. '대지'의 괴경중 (278.2~428.0g/주)은 105공 처리에서 높았으나 일장처리 간에는 차이가 없었다. 주당 괴경 수는 '수미'가 2.9~6.9개, '대지' 2.2~3.6개로 플러그 셀 크기와 일장 처리에 따른 유의성은 없었다. 80g 이상의 괴경 크기 비율이 '수미'는 32~50.9%, '대지'는 41.0~56.7%로, 두 품종 모두 288공 셀 크기에서는 감소하였고, 일장 처리 간에는 품종에 따른 차이를 보여 '수미'는 일장이 증가함에 따라 괴경 수가 증가하는 경향을 보였으나, '대지'는 일장 처리에 따른 차이를 보이지 않았다. 이상의 결과, 초기 묘 소질이 감자 생육 및 괴경 중에 영향을 줌으로써 플러그 묘를 이용한 감자 생산에 적합한 플러그 셀 크기는 162공 이하, 12시간 이상의 일장이 필요하리라 판단되었다.

This study was conducted to investigate the seasonal occurrence of chestnut weevil, Curculio sikkimensis. The chestnuts infected by the weevils were collected from Gongju, Buyeo, and Cheongyang in Chungnam Province, Republic of Korea. Investigation was focus on the escape period of chestnut weevil larvae from the fruits, invading time and vertical distribution in soil, survival rates of larvae during overwintering, emergence period, emergence rate, and sex ratio and longevity of adults. For precocious species, larvae escaped the chestnut from the mid-September to the early-October, middle species and slow species, were escaped from the late-September to the mid-October and from the early-October to the mid-November. After escaping from the chestnut fruits all of the larvae burrowed into the soil within 35 minutes. Overwintering larvae inhabited in the range of 0~48 ㎝ from the soil surface and highly distributed in the range of 18~36 ㎝. The 74.1% of wintering chamber were distributed within 18~36 ㎝ from the surface. Survival rates observed were 38.0% in 1st year, 16.0% in 2nd years, and 2% in 3rd years, respectively. Seasonal occurrence period of C. sikkimensis was from the early-Aug. to early-Oct. and the optimal occurrence period was the early and mid-Sept. Emergence rate decreased to 8.4% in 1st year, 3.6% in 2nd years, and 0.8% in 3rd years, respectively. Sex ratio was showed in the range of 0.51-0.55. It is female biased ratio. Longevity of adults was 9.9 days for female and 8.9 days for male.

도로변에는 다양한 부정형의 구조물들이 방호되지 않은 채 노출되어 있어서 통행차량에 큰 위험요소가 되고 있다. 이러한 부정형 구조물을 효과적으로 방호하는 수단으로 구조물 앞에 에너지흡수형 모듈을 적층구조로 쌓는 방법이 있다. 본 논문은 EPS블록으로 구성된 모듈타입의 충격흡수장치를 차량과 충격흡수장치간의 에너지 평형원리를 이용하여 해석하는 방법을 소개하고 0.9ton-500km/h, 0.9ton-60km/h and 0.9ton-70km/h의 충돌조건에 대한 수치 예제로 설명하였다. 이 방법은 최대가속도, 충돌변형에 걸리는 시간 모든 모듈에 대한 변형이 완료되기 전에 차량의 완전한 정지여부 등에 대한 예측을 가능하게 하지만, 모듈 수만큼의 매우 듬성듬성한 속도 및 가속도 데이터를 주기 때문에 RA와 OIV같은 안전지수를 구하기 위해서는 보간법을 이용한 데이터 수의 확대가 필요하다. 선형 및 스플라인 보간법을 이용하여 안전도를 분석하고 결과를 비교 분석하였다.

형상이 일정하지 않은 구조에 충돌하는 차량의 탑승자 안전을 확보하기 위해서 그 구조물의 앞에 공간이 허용하는 한도의 깊이만큼 충격을 흡수하는 재료로 만든 모듈을 쌓아두는 방법을 생각할 수 있다. 충격흡수모듈로 사용되기 위해서는 재료가 충분한 에너지 흡수능력을 가져야 하고 동시에 탑승자의 안전을 확보할 수 있어야 한다. 본 논문에서는 에너지 흡수능력과 더불어 탑승자의 안전을 보장하기 위하여 충격흡수재료가 가져야 할 조건을 설명하고 auard-Guard 시스템 모듈, 샌드백, 재활용 타이어, 지오컨테이너, 지오셀 그리고 EPS 블록에 대한 정적압축실험을 실시하여 그 결과를 분석하였다. 이로부터 30kg/m3의 밀도를 갖는 EPS 블록이 쿠션모듈로 사용되기 적합한 재료임을 보였다. 한편 시속 35.6km/h까지의 충돌조건으로 Drop Test를 실시하여 EPS블록의 정적특성과 동적특성간 큰 차이가 없음을 보여 주었으며 쿠션모듈로서의 성질을 개선시키기 위한 방안으로 EPS 블록에 공극을 두는 방안을 제안하고 공극이 있는 EPS블록에 대하여 Drop Test를 실시하여 EPS 블록을 이용한 충격흡수시설의 설계에 필요한 재료적 특성치를 제시하였다.

In spray pyrolysis, the effects of the preparation temperature, flow rate of the carrier gas and concentration of the spray solution on characteristics such as the morphology, size, and emission intensity of Ca8Mg(SiO4)4Cl2:Eu2+ phosphor powders under long-wavelength ultraviolet light were investigated. The phosphor powders obtained post-treatment had a range of micron sizes with regular morphologies. However, the composition, crystal structure and photoluminescence intensity of the phosphor powders were affected by the preparation conditions of the precursor powders. The Ca8Mg(SiO4)4Cl2:Eu2+ phosphor powders prepared at temperatures that were lower and higher than 700˚C had low photoluminescence intensities due to deficiencies related to the of Cl component. The phosphor powders with the deficient Cl component had impurity peaks of Ca2SiO4. The optimum flow rates of the carrier gas in the preparation of the Ca8Mg(SiO4)4Cl2:Eu2+ phosphor powders with high photoluminescence intensities and regular morphologies were between 40 and 60 l/minute. Phosphor powders prepared from a spray solution above 0.5 M had regular morphologies and high photoluminescence intensities.

본 논문에서는 프리스트레스 콘크리트(PSC) 거더교의 긴장력 손실을 예측하기 위한 진동기반 모니터링 체계를 제안하였다. 제안한 체계는 긴장력 손실 경보 단계와 긴장력 손실 정도를 평가하는 단계로 구성하였다. 먼저, 긴장력 손실 경보를 위해 두 위치에서 취득된 주파수 응답의 변화를 사용하여 긴장력 손실의 발생을 모니터링하는 새로운 전역적 손상경보기법을 제안하였다. 제안된 기법은 응답신호의 파워스펙트럼만을 이용하기 때문에 별도의 모드해석과정 없이 실시간으로 손상경보가 가능하다. 다음으로, 긴장력 손실 정도를 평가하기 위하여 고유진동수의 변화로부터 긴장력의 상대적인 손실 정도를 평가할 수 있는 긴장력 손실 예측 기법을 선정하였다. 제안된 체계의 유용성을 축소 모형 PSC 거더에 대한 실험을 통해 평가하였다.

본 연구는 2005년부터 2007년까지 파주, 강화도, 이천, 시화호, 충주 등 5개 지역에 서식하는 수리부엉이 9쌍의 둥지를 정기적으로 촬영, 관찰하여 번식생태와 교미행동을 연구하였다. 한배산란수는 1~4개로 평균 2.77개(n=15)였으며, 부화성공률은 약 71%(n=34)였고, 이소성공률은 약 83%(n=23)였다. 수리부엉이는 산란이 끝난 후에도 암수간의 교미행동이 지속되며, 새끼들이 둥지를 떠나는 시기인 부화 후 7~8주까지 교미행동을 계속한다는 것을 확인하였다. 이러한 수정 목적 이외의 교미 행동은 암컷과 수컷 모두의 필요에 의해서 이루어지는 것으로써 '부부관계(pair-bond)'의 확인, 유지, 강화를 통해 새끼를 안정적으로 키우기 위한 것으로 해석된다.

본 연구에서는 볼트의 변형을 고려한 강재 조립 합성보의 휨거동에 대한 해석기법 및 결과가 제시되었다. 볼트의 변형, 합성효과 및 접촉면의 마찰이 합성보의 휨거동에 끼치는 영향을 파악하였다. 볼트의 변형이 합성보의 휨거동에 끼치는 영향을 고려하기 위하여, 구조해석 프로그램인 ABAQUS의 Nonlinear Spring요소를 사용하였으며 볼트의 변형을 고려하지 않은 결과와 비교하였다. 유한요소 모델에 의해서 처짐, 휨응력, 전단응력이 계산되었으며 이런 결과는 완전 비합성보, 부분 합성보 및 완전 합성보의 해석 값과 비교되었다. 해석결과 합성보의 거동은 강재의 마찰보다 볼트의 갯수로 표현되는 합성률에 크게 영향을 받았다. 특히 합성률이 50%이상이 되면 볼트의 변형을 고려한 합성보의 휨거동은 완전합성보와 유사하게 나타났다.

Ni-20Cr-20Fe-5Nb alloy with or without was manufactured by mechanical alloying process and consolidated by spark plasma sintering (SPS). The grain size of the alloy with was smaller than that of alloy without which results from the effect of suppressing grain growth. The tensile strength at room temperature was increased by the addition of but decreased abruptly at temperature above . It seems to result from the change of deformation mechanism due to fine grain size, that is, grain boundary sliding is predominant at above while internal dislocation movement is predominant at below . After conventional heat treatment process of solution treatment and aging, a small amount of phase was formed in Ni-20Cr-20Fe-5Nb alloy while a large amount of was formed in Inconel 718 in the previous report. This is due to exhaustion of Nb content by the formation of NbC during consolidation.