갈대의 성숙종자로부터 최적 조직배양조건을 확립하기 위하여 종자로부터 캘러스 유도 및 식물체 재분화 체계를 확립하였다. 종자로부터 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 1.0 mg/L 2,4-D와 0.5 mg/L BA가 첨가된 MS 배지에서 가장 높은 빈도로 캘러스가 유도되었다. 캘러스로부터 식물체 재분화는 cytokinin류인 BA와 kinetin이 첨가되지 않은 MS 배지에서 59.6%로 높은 재분화 효율을 나타내었

This study aims to provide preliminary review for standardization of methods for evaluating reduction performance of pollution-reducing building materials and to select commercially available pollution-reducing building materials for assessing their current status. The ISO 16000-23 and -24 standards were used as references for standardizing the test methods. The sub test categories—stability of the supply air concentration, type of the supply air, and supply air concentration—which were not included in the standards were selected and conducted for the purpose of this study. Five (5) wall materials and 2 ceiling materials were tested for formaldehyde reduction performance and 3 wall materials were tested for toluene reduction performance. The study included experimental review of the essential test criteria such as stability of the supply air, internal stability of the chamber, and recovery rate. The samples tested exhibited some reduction performance for formaldehyde but little reduction performance for toluene.

본 연구는 낙동강 하구역의 사주지형 변화를 예측하기 위한 기초적 연구로서 하천유량의 변화에 따른 부유사의 물질수지에 대하여 검토하였다. 낙동강 하구역의 부유사 물질수지는 갈수기시 60,708 kg/day가 유입하고, 홍수기는 306,892 kg/day가 외해를 통하여 유출하는 것으로 산정되었다. 낙동강 하구역은 전반적으로 갈수기 및 평수기에 부유사의 유입, 홍수기에 부유사의 유출이 반복 되지만, 사주지형 주변에서는 부유사의 유입과 유출 거동이 복잡하게 나타나 장기 지형변동 모니터링이 필요한 것으로 나타났다.

Bacillus thuringiensis (Bt) is a bacterial biopesticide against insect pests, mainly lepidopterans. Spodoptera exigua and Plutella xylostella exhibit significant decreases in Bt susceptibility in late larval instars. To enhance Bt pathogenicity, we used a mixture treatment of Bt and other bacterial metabolites which possessed significant immunosuppressive activities. Mixtures of Bt with culture broths of Xenorhabdus nematophila (Xn) or Photorhabdus temperata ssp. temperata (Ptt) significantly enhanced the Bt pathogenicity against late larval instars. Different ratios of Bt to bacterial culture broth had significant pathogenicities against last instar P. xylostella and S. exigua. Five compounds identified from the bacterial culture broth also enhanced Bt pathogenicity. After determining the optimal ratios, the mixture was applied to cabbage infested by late ins tar P. xylostella or S. exigua in greenhouse conditions. A mixture of Bt and Xn culture broth killed 100% of both insect pests when it was sprayed twice, while Bt alone killed less than 80% or 60% of P. xylostella and S. exigua, respectively. Other Bt mixtures, including Ptt culture broth or bacterial metabolites, also significantly increased pathogenicity in the semi-field assays. These results demonstrated that the Bt mixtures collectively names 'Bt-Plus' can be developed into potent biopesticides to increase the efficacy of Bt.

Odors were produced in the reactor where contained the swine excreta sampled from the pigery and were analyzed the major odor materials based on the odor contribution index. The container hoggery where six pens were separated was set up in S-myun, Gyeonggi-do, Korea: one was control group, the other were experimental group. Sixty weaners were raised and their excreta were sampled every week to the test of the effect of feed type probiotics. Some probiotics showed very effective deodorizing activity during the whole experimental periods. All products had positive effects on the reducing odor intensities after 4 to 5 week periods. The odor contribution analysis represented the main odor compounds were trimethylamine and volatile sulfur compounds such as hydrogen sulfide, mercaptane, and dimethyl sulfide.

Though food safety efforts of the government has been increasing, but the health hazards resulting from food does not stop and thus increases consumers' anxiety and distrust of government. For more reliable safety management, developing analysis and intake measurement methods and strengthening regulatory control with strict criteria is necessary. In addition, as not only the known hazards by now, but also unknown but obvious hazards are simultaneously ongoing in current environments, the unified risk management and health assessment for food, health food and medicines from the media is critical. As risks and damage to health may show up after a long time after exposure,preventive measures and strategies for safety control is also needed.

This study was performed to develop new anti-obesity agents from medicinal plants for functional food industry. We prepared lipase inhibitor-containing ethanol extracts from Desmodium oxyphyllu DC. and its nutritional characteristics, stability of temperature and pH and physiological functionalities were investigated. Crude lipid content and curde protein content of ethanol extract from Desmodium oxyphyllum DC. were 26.5% and 5.7%, respectively and its asparagine content was 4, 860 ㎎/100 g, dry basis. The ethanol extracts were also stable from pH 3.0 to pH 9.0 and below 80℃. The ethanol extract were showed high lipase inhibitory activity of 74.2% and acetylcholinesterase inhibitory activity of 68.4%.

In this study was analyzed substances that high contribute to odor strength degree which emitted from the painting, the plating, the casting, the rubber manufacturing, and the used oil refining facilities in Gwangju Hanam industrial complex. In order to this, we analyzed two types of nitrogen compounds, five types of sulfur compounds, thirteen types of the aldehyde, and ten types of volatile organic compounds discharged from an outlet for antipollution facilities. The results are as following high contribution rate sustances to odor strenth was ordered butylaldehyde (73.8%) ＞ acetaldehyde (17.7%) ＞ propionaldehyde (4.9%) at painting facilities. At plating facilities, it was ordered Acetaldehyde (59.0%) ＞ ammonia (19.1) ＞ hydrogen sulfide (13.1%). At casting facilities, it was ordered Hydrogen sulfide (65.9%) ＞ acetaldehyde (17.2%) ＞ sulfur dioxide(5.2%). At rubber manufacturing facilities, it was ordered i-valeraldehyde (35.0%) ＞ butylaldehyde (32.0%) ＞ n-valeraldehyde (13.3%) and at used oil refinery facilities, it was ordered Acetaldehyde (36.8%)＞ butylaldehyde (33.6%) ＞ sulfur dioxide (14.5%).

This study is conducted to screen compounds affecting ruminal fermentation under in vitro incubation. Saponin, chitosan, metformin, zinc acetate, zinc chloride, zinc oxide, zinc sulfate, ascorbic acid, oil-coated ascorbic acid, nicotinic acid, eastern herb cocktail, charcoal extract and garlic sources (lyophilized and extract) were added to rumen culture fluid at 1.25% of substrate (100% timothy) volume at 24 h incubation. pH, total gas, ammonia, VFAs were measured. Ascorbic acid increased total gas production indicating fermentation level. All zinc compounds significantly decreased (p<0.05) although zinc sulfate increased propionate of volatile fatty acid (p<0.05). In following experiment, ascorbic acid, oil-coated ascorbic acid, garlic lyophilized, herb cocktail and zinc sulfate were added to rumen culture fluid at 2.5% of substrate with 3, 6, 12 and 24 h incubation. Zinc sulfate decreased both ruminal fermentation and VFAs production but ascorbic acid enhanced total gas production. Ascorbic acid increased fermentation regardless of supplement concentration although excessive zinc sulfate decreased fermentation. These results suggest that optimal level of trace compounds might affect ruminal fermentation in ruminant.

도로에서의 강우유출수 내 포함된 오염물은 주요한 비점오염원으로 간주되고 있으며 비점오염물질 저감을 위하여 많은 대책이 적용되고 있다. 도로청소에 의한 방법 또한 오염물의 강우유출에 의한 배출양을 감소시킬 수 있는 대책 중의 하나로 인식되는 추세에 있다. 본 연구에서는 도로청소 시 수거된 토사입자들의 특성을 파악하고 퇴적토사에 함유된 오염물질의 농도를 분석하였다. 분석결과를 바탕으로 하여 도로 구간별로 도로청소에 의하여 저감할 수 있는 오염물의 양을 평가하였다. 도로청소를 통하여 수거된 퇴적토사는 모래질로 분석되었으며 세립토 비중은 낮은 결과를 보였다. 퇴적토사 내 오염물의 양은 토양환경기준을 초과하지 않았으나 청소작업시 물을 살수함으로써 토사에 포함된 오염물이 세척되어 침출수로 미리 배출되는 것에 기인한다고 판단된다. 두 도로구역에 대하여 오염물 농도, 발생 퇴적토사량, 오염물 원단위를 적용하여 저감할 수 있는 오염물 양을 평가한 결과, ○○지역에 대하여 TSS 31.4% 및 △△지역에서는 TSS 7.7%의 TSS 저감량이 도출되었다. 중금속과 같은 타 오염물의 경우 훨씬 낮은 저감량이 평가되었으나 이는 물의 살수로 인하여 퇴적토사 내 오염물이 침출수로 배출된 것에 기인한다고 판단된다. 보다 정확하게 도로청소에 의하여 저감할 수 있는 오염물의 양을 산정하기 위하여 보다 자세하고 체계적인 연구가 필요하다고 판단된다.

초임계 CO2유체를 이용하여 미강 중 표면활성물질을 추출하고 추출물의 표면활성능을 최적화하는 추출 조건을 반응표면분석법을 통해 조사하고자 하였다. 추출수율은 독립변수인 압력, 온도, 보조용매량이 많을수록 높았으며, 보조용매량이 추출수율에 가장 큰 영향을 주었다. 회귀분석을 통해서 얻은 최적 추출 조건은 추출압력 330 bar, 추출온도 65oC, 보조용매량 250 g이었다. 표면활성능 지표인 계면장력은 추출압력과 추출온도가 증가할수록 그리고 보조용매량이 높을수록 낮았으며, 추출수율과 마찬가지로 보조용매량이 계면장력에 가장 큰 영향을 주었지만 추출압력과 추출온도 등의 변수에 의한 영향은 비교적 적었다. 회귀분석을 통해서 얻은 최적 추출 조건(낮은 계면장력)은 추출압력 350 bar, 추출온도 65oC, 보조용매량 50 g이었다. 또한 D-optimal design을 통해 얻은 실험 결과를 바탕으로 회귀분석을 하였을 때 예측모델식은 실제 측정값과 비교해 높은 유의성을 나타내는 것으로 판단되었다. 보조용매량이 많을수록 극성 물질이 더 많이 추출되어 낮은 계면장력 값을 예상하였지만 실제 측정 결과 보조용매량이 가장 낮은 조건인 50 g에서 계면장력은 가장 낮게 측정되었다. 이의 규명을 위하여 TLC 및 HPLC 분석을 통한 추출물에 대한 성분 조사, 추출물을 이용한 유화액 제조, 유화액 특성 평가 등 추가 실험이 필요한 것으로 사료되었다.

The aim of study was to investigate polluted amounts 16 PAHs in railroad(waste railway ties) area, factory area and land-fill area. All of the samples were analyzed by GC-MSD(SIM mode), and the recovery range, detection limit and standard deviation obtained by this experiment were 73.88 ~ 94.75%, 0.009 ~ 2.252 μg/kg and 1.861 ~ 12.373, respectively. The concentrations of total PAHs(t-PAHs) and total carcinogenic PAHs(t-PAHcarc) in soils of three area were in the range of 12.54 ~ 3274.95 μg/kg on a wet weight basis with a mean value of 499.8 μg/kg and 0 ~122.77 μg/kg with a mean value of 20.16 μg/kg, respectively. The correlation between t-PAHs and t-PAHcarc appeared very high in railroad(waste railway ties) area (R2 = 0.8301), factory area (R2 = 0.9217) except land-fill area(R2 = 0.3782), indicated that t-PAHcarc concentration increases in proportion with t-PAHs.