Beet armyworm (BAW), Spodoptera exigua (Hübner), is a polyphagous lepidopteran pest mainly distributed in tropical and subtropical regions. BAW are known to migrate long distances to find suitable host plants and environmental condition. The possibility of its overwintering in temperate regions has been suspected. This study was conducted for investigating overwintering and phenology of BAW using by sex pheromone trap in Jeju during 2007 to 2011. BAW was able to overwinter at pupae stage regardless of temperature of winter season in Jeju. The times of BAW caught first on traps were different by surveyed years - early March in 2007, middle March in 2008 and 2011 and early April in 2009 and 2010. The times of BAW caught on trap tended to be more dependent on temperature variation during on November and December than on mean temperature of winter season. The peak season of adults of the overwintering generation was early to middle of May. The results of seasonal periodicity of BAW monitored by sex pheromone trap during 2009 and 2010 indicated that five generations were passed in Jeju. The peak season of 1st generation was on middle of April, 2nd generation on middle of July, 3rd generation on early of August, 4th generation on late of August and 5th generation on middle of September. There were similar patterns by different above sea levels except the season of overwintering generation adults occurrence (March to May).

Proteomics may help to detect subtle pollution-related changes, such as responses to mixture pollution at low concentrations, where clear signs of toxicity are absent. Also proteomics provide potential in the discovery of new sensitive biomarkers for environmental pollution. We utilized SELDI-TOF MS (surface enhanced laser desorption. / ionization time-of-flight mass spectrometry) to analyze the proteomic profile of Heterocypris incongruens exposed to several heavy metals (lead, mercury, copper, cadmium and chromium) and pesticides (emamectin benzoate, endosulfan, cypermethrin, mancozeb and paraquat dichloride). Several highly significant biomarkers were selected to make a model of classification analysis. data sets obtained from H. incongruens exposed to pollutants were investigated for differential protein expression by SELDI-TOF MS and decision tree classification. Decision tree model was developed with training set, and then validated with test set from profiling data of H. incongruens. Machine learning techniques provide a promising approach to process the information from mass spectrometry data. Even thought the identification of protein would be ideal, class discrimination does not need it. In the future, this decision tree model would be validated with various levels of pollutants to apply field samples.

This study was conducted to examine the potential of surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to screen Tetranychus urticae resistance to pyridaben and dicofol. T. urticae is one of the most important pests in greenhouse and orchard, and huge expense is needed to control because of its strong resistance to acaricides. Consequently speedy and accurate monitoring of acaricidal resistance is the key factor of IPM for T. urticae. SELDI-TOF MS is a novel approach to biomarker discovery that combines two powerful techniques: chromatography and mass spectrometry. It can provide a rapid protein expression profile of acaricidal sensitive and resistant T. urticae. In this study we had different protein and peptide patterns between sensitive and resistant strains to pyridaben and dicofol. In the future this results could be a useful data to develop a good monitoring tool of site and host specific mite resistance to various acaricides.

Metabolomics aims at the comprehensive, qualitative and quantitative analysis of wide arrays of endogenous metabolites in biological samples. It has shown particular promise in the area of toxicology and drug development, functional genomics, system biology and clinical diagnosis. In this study, analytical technique of MS instrument with high resolution mass measurement, such as time-of-flight (TOF) was validated for the purpose of investigation of amino acids, sugars and fatty acids. Rat urine and serum samples were extracted by selected each solvent (50% acetonitrile, 100% acetonitrile, acetone, methanol, water, ether) extraction method. We determined the optimized liquid chromatography/time-of-flight mass spectrometry (LC/TOFMS) system and selected appropriated columns, mobile phases, fragment energy and collision energy, which could search 17 metabolites. The spectral data collected from LC/TOFMS were tested by ANOVA. Obtained with the use of LC/TOFMS technique, our results indicated that (1) MS and MS/MS parameters were optimized and most abundant product ion of each metabolite were selected to be monitorized; (2) with design of experiment analysis, methanol yielded the optimal extraction efficiency. Therefore, the results of this study are expected to be useful in the endogenous metabolite fields according to validated SOP for endogenous amino acids, sugars and fatty acids.

Bacillus thuringiensis (B. t.) strains are important microorganism because they produced a large amount of δ-endotoxin protein per bacterial cell and their toxins are highly toxic to Lepidoptera, Coleoptera, and Diptera depending on B. t. To date, more than a hundred Cry proteins have been identified and classified into 195 holotypes, based on the amino acid sequence identity. The Cry proteins or cry genes from the Korean native B. t. isolates in this study were not identified yet. The electrospray ionization of quadrupole time of flight mass spectrometry (ESI Q-TOF MS) was used to get the internal amino acid sequences of the endotoxin-spore culture mixtures of B. t. isolates, for which polymerase chain reaction (PCR) techniques were unable to detect the cognate genes. Most of Cry proteins seperated, excized, and extracted from the one dimensional - polyacrylamide gel electrophoresis (1D-PAGE), instead of 2D-PAGE, were matched with protein databases using MS-MASCOT search program. The internal amino acid sequences which were submitted to protein BLAST (basic local alignment search tool) had partially homology with the Cry protein databases. Hence, present data strongly suggest that the de novo amino acid sequencing and ESI Q-TOF/MS analysis along with MASCOT search could be used as a simple and rapid method for detection of novel Cry toxins from B. t. isolates and identification of B. t. isolates.

기존의 전문성 연구들은 참가자들에게 일상적인 과제를 수행하게 함으로써 전문가와 초보자들의 차이를 알아보고자 하였다. 하지만, 본 연구는 일상적 비행 상황에 익숙해진 조종사들이 이전에 경험해보지 못한 새로운 비행 상황에서도 유연하게 대처할 수 있는지 알아보고자 하였다. 즉, 조종사의 숙련정도에 따라 정상적 및 비정상적 상황에서의 상황인식 능력이 차이가 나는지 관찰하였고 추가적으로, 전문성이 자극 제시 시간에 따른 수행변화에 영향을 주는지 알아보았다. 이를 위해 42명의 조종사들을 전문성의 정도에 따라 초보자와 전문가 집단으로 나누고 이들의 수행이 상황의 특수성 및 자극 제시 시간에 따라 어떻게 달라지는지 알아보았다. 연구를 위하여 조종실 계기판을 모방한 동영상이 제작되었는데, 동영상에 보여지는 계기판들이 모두 정상으로 움직이는 정상적 상황과 이들 중 하나의 계기가 오작동을 일으키는 비정상 상황이 연출되었다. 또한 상황을 지각하기 위한 시간의 차이가 전문성에 따라 다른 효과를 가져오는지 살펴보기 위해서 계기판 상황이 오랫동안 제시되는 긴 제시 조건과 상대적으로 짧게 제시되는 조건으로 조작되었다. 실험 결과, 정상적인 상황에서는 초보자와 전문가 집단 사이의 상황 인식이 유의한 차이가 없었으나, 비정상적인 상황에서는 전문가 집단의 상황인식이 초보자 집단보다 유의하게 높은 것으로 나타났다. 또한, 비행 상황의 제시 시간이 짧아졌을 때 조종사들의 전반적인 상황인식 정확도가 떨어졌지만, 전문가와 초보자의 수행차이는 변함없이 초보자 집단에 비해 전문가 집단의 높은 수행이 유지되었다. 비행 상황의 특수성과 제시 시간의 상호작용에 따라 초보자와 전문가의 상황인식은 어떻게 달라지는지 보았을 때, 비행 상황의 제시 시간이 길고 짧음에 관계없이 정상적 상황에서는 초보자와 전문가 집단의 상황인식 차이가 나타나지 않았으나, 비정상적 상황에서는 전문가 집단의 상황인식 정확도가 초보자 집단 보다 유의하게 높은 것으로 나타났다. 전반적으로 볼 때, 비정상적 상황은 전문가 집단의 수행에 미치는 영향에는 큰 영향을 미치니 못하나 초보자 집단의 수행에는 현저한 영향을 미치는 것으로 나타났다.

조종사의 비행수행능력과 자존감이 비행비교 상황에서 위험행동에 미치는 영향을 알아보기 위해 비행교육과정 조종사 48명을 대상으로 시뮬레이터 상황실험을 실시하였다. 피험자간 변인으로서 비행수행능력(비행등수)과 자존감 척도로 각각의 높고 낮은 그룹으로 나누어 안전규칙상 착륙이 허가되지 않는 활주로 접근 시뮬레이션 상황을 수행하게 하였다. 접근 상황은 피험자 내 설계로 세 번에 걸쳐 측정하였고, 동일한 각각의 상황실험 수행 직전에 실험참가자에게 다른 사람들의 수행결과를 조작(긍정비교, 부정비교, 통제)하여 제시하였다. 실험참가자의 위험행동은 접근한 고도를 통하여 측정하였다. 실험 결과 각 제시 조건에 따라 위험행동이 유의미하게 차이가 나타났고, 각 비교조건에서의 위험행동 경향은 비행수행능력과 자존감의 수준에 따라 달라지는 상호작용 효과가 나타났다. 비행수행능력이 낮은 집단은 비교조건들에서 통제조건과 달리 위험한 행동을 하는 것으로 나타났고 비행수행능력이 높은 집단은 부정비교조건에서 다른 조건들과 달리 위험을 회피하는 경향을 보였다. 자존감이 높은 집단은 통제조건에 비해 비교조건들에서 유의미한 위험행동을 보인 반면, 자존감이 낮은 집단은 다른 조건들에 비해 부정적 비교조건에서 유의미한 위험회피행동을 보였다.

This study was conducted with respect to the causal factors revealed through the investigation of the recent airlines aircraft crash accident which occurred while aircraft was on the climb-out or on the final approach. This study also highlighted the importance of flight deviation and exceedance occurrences in consideration of Flight Operational Quality Assurance Program(FOQA). Twenty airline pilots participated in the flight experiment to perform ten(10) sets of simulated approaches and landings. As a result, Twelve(12) kinds of deviation events were discovered. In this respect, The FOQA program must be fully implemented to prevent any flight safety incident under the auspices of the Korea domestic aviation community as well.

Imago"s flight activities of Protaetia brevitarsis were from mid June to early September, and those of Allomyrina dichotoma were from mid June to late August. These activities were mainly influenced by amount and duration of rain. They were more active when there was small amount and short period of rain in a year. Distribution depth of the 3<SUP>rd</SUP> larvae of P. brevitarsis were not affected to the temperature. But A. dichotoma were sensitively react to the temperature, almost larvae were near the surface when high temperature. The distribution of P. brevitarsis was not affected by humidity, but most of A. dichotoma were near the surface when high humidity. Larvae of P. brevitarsis usually ate their dead individuals. When we supplied as food the pork, chicken, fresh mackerel, bread, apple, and pear. They took all of these food but larvae of A. dichotoma took only bread. Two species were lived in similar environment but those feeding habit was very different.

Correlations between in-flight particle, splat and coating microstructure of thermally sprayed Ni20Cr were investigated. Flame spray and arc spray systems were employed for spraying Ni20Cr powder and Ni20Cr wire, respectively. The results showed that the arc spray process produced a broader size distribution for both in-flight particles and splats compared to flame process. Flower-like splat morphology was obtained from the arc spray whereas a pancake-like splat was obtained by flame spray. Ni20Cr coating sprayed by arc process had a denser microstructure, lower porosity and better adhesion at the interface.

NiTiZrSiSn bulk metallic glass powder was produced using inert gas atomization and then was sprayed onto a SS 41 mild steel substrate using the kinetic spraying process. Through this study, the effects of thermal energy of in-flight particle and crystallization degree by powder preheating temperature were evaluated. The deformation behavior of bulk metallic glass is very interesting and it is largely dependent on the temperature. The crystalline phase formation at impact interface was dependent on the in-flight particle temperature. In addition, variations in the impact behavior need to be considered at high strain rate and in-flight particle temperature.