The mu opioid receptor (MOR) has been regarded as the main site of interaction with analgesics in major clinical use, particularly morphine. The repressor element-1 silencing transcription factor (REST) functions as a transcriptional repressor of neuronal genes in non-neuronal cells. However, it is expressed in certain mature neurons, suggesting that it may have complex and novel roles. In addition, the interactions between MOR and REST and their functions remain unclear. In this study, we examined the effects of morphine on the expression of REST mRNA and protein in human neuroblastoma NMB cells to investigate the roles of REST induced by MOR activation in neuronal cells. To determine the effects of morphine on REST expression, we performed RT-PCR, real-time quantitative RT-PCR, western blot analysis and radioligand binding assays in NMB cells. By RTPCR and real-time quantitative RT-PCR, the expression of REST was found to be unchanged by either the MOR agonist morphine or the MOR specific antagonist CTOP. By western blot, morphine was shown to significantly inhibit the expression of REST, but this suppression was completely blocked by treatment with CTOP. In the radioligand binding assay, the overexpression of REST led to an increased opioid ligand binding activity of endogenous MOR in the NMB cells. These results together suggest that morphine inhibits the expression of REST in human neuroblastoma cells through a post-transcriptional regulatory mechanism mediated through MOR.

Few studies have evaluated the apoptosis-inducing efficacy of NaF on cancer cells in vitro but there has been no previous investigation of the apoptotic effects of NaF on human oral squamous cell carcinoma cells. In this study, we have investigated the mechanisms underlying the apoptotic response to NaF treatment in the YD9 human squamous cell carcinoma cell line. The viability of YD9 cells and their growth inhibition were assessed by MTT and clonogenic assays, respectively. Hoechst staining, DNA electrophoresis and TUNEL staining were conducted to detect apoptosis. YD9 cells were treated with NaF, and western blotting, immunocytochemistry, confocal microscopy, FACScan flow cytometry, and MMP and proteasome activity assays were performed sequentially. The NaF treatment resulted in a time- and dose-dependent decrease in YD9 cell viability, a dose-dependent inhibition of cell growth, and the induction of apoptotic cell death. The apoptotic response of these cells was manifested by nuclear condensation, DNA fragmentation, the reduction of MMP and proteasome activity, a decreased DNA content, the release of cytochrome c into the cytosol, the translocation of AIF and DFF40 (CAD) into the nucleus, a significant shift of the Bax/Bcl-2 ratio, and the activation of caspase-9, caspase-3, PARP, Lamin A/C and DFF45 (ICAD). Furthermore, NaF treatment resulted in the downregulation of G1 cell cyclerelated proteins, and upregulation of p53 and the Cdk inhibitor p27KIP1. Taken collectively, our present findings demonstrate that NaF strongly inhibits YD9 cell proliferation by modulating the expression of G1 cell cycle-related proteins and inducing apoptosis via mitochondrial and caspase pathways.

여름철 에너지 절약, 온실가스 줄이기, 직장인의 건강증진 등을 위한 쿨맵시 캠페인에 대하여 범국민 인식 및 실천의 필요를 바탕으로, 본 연구는 착의실험을 통해 클맵시 권장복장 착용시의 생리적 반응 및 주관적 감각에 대해 분석하였다. 1차 실험은 두 복장, 즉 일반복장, 쿨맵시 권장복장에 대한 생리적 반응의 측정으로서, 두 환경기온 25℃와 27℃, 상대습도 50%R.H.에서 20대 성인남성 4명을 대상으로 실험을 행하였다. 일반복장은 긴팔 셔츠 정장바지 차림이었고 쿨맵시 권장복장은 넥타이 없이 반팔셔츠에 정장바지 차림이었다. 피험자는 30분간의 안정기를 가진 후 60분 동안 실험을 실시하였는데 사무실 작업과 유사한 컴퓨터 워드작업을 행하였고, 피부온, 직장온, 의복하 습도, 발한량, 온열감, 습윤감, 쾌적감 등을 측정하였다. 대부분의 반응에서 25℃ 일반복장의 경우와 27℃ 쿨맵시복장의 경우가 유사한 결과를 나타내고 우수한 것으로 나타났다. 25℃ 쿨맵시복장의 경우에는 저강도 작업이 지속되면 직장온의 저하가 우려되었으며 27℃ 일반복장에서는 고 평균 피부온, 고 발한량, 높은 온열감 등을 보였다. 2차 실험은 일반복장을 착용한 채 환경온도를 점진적으로 하강시키면서 권장 여름철 냉방온인 27℃에서 쿨맵시 권장복장을 착용한 경우의 피부온도를 발현시키는 실내 환경온도를 찾는 것이었다. 그 결과 권장복장 경우의 피부온을 나타내려면 일반복장의 경우에는 환경온을 2℃를 더 낮추어야만 하였다. 여름철 실내 환경온을 27℃로 높이고 쿨맵시 권장복장을 착용하는 것이 사무실의 장시간 저강도 작업 하에서는 피부온, 주관적 온열감이 우수하였다.

Korean logistics industry have been focused on transportation business. However, with the expansion of the electronic commerce and on-line shopping, delivery service is now dramatically growing. Despite the expansion of logistics market, the domestic logistics industry have significant structural problems such as low productivity comparing with the advanced countries, relatively high cost and shortage of human resources and lack of professionalism of people in the industry. Logistics companies reallocate employees, use subcontractors, expand consignment and training the employees to overcome the labor shortages but it has some limits. In recognition of the importance of labor in the logistics industry, financial support and investment have increased. Logistics companies tend to hire consultants, set up logistics department or R&D center in order to establish highly productive logistics process and system so it is viewed that there will be considerable demands of human resources in the logistics industry. This study indicates implications and development direction of human resources in the logistics industry by looking into prospect and characteristic of the industry, employment status, training programs and qualification requirements.

The origin of squamous cell components in salivary gland tumor has been not yet clarified in detail. The squamous cell differentiation from adenocarcinoma has been reported in various carcinoma by HPV transfection in vitro. The adenocarcinoma cells adjacent to the squamous cell carcinoma components were positive for HPV. This is thought to indicate that after adenocarcinoma cells are transfected with HPV, they undergo morphological changes, and that squamous cell differentiation follows. The purpose of this study were to examine the effects of HPV-16 E6/E7 gene transfection into SGT cell line from human salivary gland adenocarcinoma, and to study the relation between the E6/E7 gene and squamous differentiation. Plasmid pBR322 containing HPV-16 was transfected into cultured SGT cell line using lipofectin method. Hygromycin was used as a selection marker. The presence of HPV E6/E7, transglutaminase 1, and involucrin mRNAs and protein in E6/E7 gene transfected cells was investigated by RT-PCR and immunoslot blot method. The apoptosis index was analysed by flow cytometry. The growth rate of E6/E7 gene transfected cells was reduced. E6/E7 transfected SGT cells increased apoptosis index. Involucrin and TGase I mRNAs by the squamous cell differentiation was most conspicuous in the E6/E7 gene transfected cell compared with non transfected cells. Squamous cell differentiation demonstrated in the transfectedSGT cell line, which expressed E6/E7 fusion gene mRNA.E6/E7 gene transfected cells showed squamous cell differentiation, expressing involucrin and TGase 1 protein by immunoslot blotting. The transfected SGT cell which expressed E6/E7 gene mRNA showed the squamous cell differentiation particularly clearly, and apoptosis was also demonstrated. It suggested that E6/E7 gene transfection into human salivary gland adenocarcinoma cells might induce clear squamous cell differentiation and contribute to study the pathogenesis of human salivary gland adenocarcinoma.

Over the years, scientists have developed many test methods to evaluate the efficacy of skin care products. The needs for objective assessment have stimulated to develop instruments that are capable of reliably monitoring some parameters in evaluating skin conditions. The beauty is evaluated as a measure of smoothness of skin surface. Quantitative size measurements of skin pores is also important concept to evaluate the their conditions. The purpose of this paper is to measure the temperature change of skin and the size of pores in the skin. The pore sizes were changed by its varying skin temperature. They were decreased by applying a essence which is contained with propellant and contents.

Oral squamous cell carcinoma (SCC) is one of the leading causes of cancer mortality worldwide. It is generally thought that adjuvant chemotherapy provides modest prolongation of survival in various carcinoma. Docetaxel (Taxotere, TXT) play a significant role in the treatment of various solid tumors of epithelial origin. CsA (immunosuppressive drug) was widely used as adjunct for the treatment of cancer. Thus, it is important to pursue the apoptosis of IHOK and oral SCC induced by TXT combined with CsA related to the pathogenesis of oral SCC. But TXT combined CsA effect on IHOK and oral SCC remains unclear. After cultured IHOK and HN 22 oral squamous cell carcinoma cell line treated by 10 nM TXT and 1 μM, and caspase inhobitor, respectively, apoptosis index, cytochrome c and caspase-3 -8, -9 mRNA expression by RT-PCR, and procaspase-3 protein amount by immunoslot blotting was prepared. The purpose of this study were to examine the TXT-induced apoptosis pathway via caspase activation by CsA enhancement, and to apply these results to an effective therapeutic treatment plan for oral SCC by TXT combined CsA . 10 nM TXT showed about 60%, 55% celluar apoptosis of IHOK and HN 22, cell line, respectively, while CsA alone did not induce apoptosis in IHOK and HN 22 cell line. 1 μM CsA combined with 10 nM TXT increased apoptosis in IHOK and HN 22 cell line through caspase-3 and cytochrome c mRNA expression, while could not effect on caspase-8 and -9. Caspase inhibitor suppressed apoptosis of IHOK and HN 22 cell line induced by a combination of 1 μM CsA and 10 nM TXT. Immnoslot blotting showed procaspase-3 activation by a combination 1 μM CsA and 10 nM TXT, while caspase inhibitor inhibited activation. It suggested that a combination of CsA and TXT might induce increased apoptosis of IHOK and HN 22 oral squamous cell carcinoma cell line through caspase-3 activation. This treatment with a combination of TXT and CsA may be an effective therapeutic strategy for oral squamous cell carcinoma

Periodontal disease is a major oral disorder and comprises a group of infections that lead to inflammation of the gingiva and the destruction of periodontal tissues. PPARy plays an important role in the regulation of several metabolic pathways and has recently been implicated in inflammatory response pathways. However, its effects on periodontal inflammation have yet to be clarified. In our current study, we evaluated the anti-inflammatory effects of PPARy on periodontal disease. Human gingival fibroblasts (HGFs) treated with lipopolysaccharide (LPS) showed high levels of intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), matrix metalloproteinase-2 (MMP-2), and -9 (MMP-9). Moreover, these cells also showed upregulated activities for extracellular signal regulated kinase (ERK1/2), inducible nitric oxide synthase (iNOS) and cyclooxygnase-2. However, cells treated with Ad/PPARy and rosiglitazone in same culture system showed reduced ICAM-1, VCAM-1, MMP-2, -9 and COX-2. Finally, the anti-inflammatory effects of PPARy appear to be mediated via the suppression of the ERK1/2 pathway and consequent inhibition of NF-kB translocation. Our present findings thus suggest that PPARy indeed has a pivotal role in gingival inflammation and may be a putative molecular target for future therapeutic strategies to control chronic periodontal disease.

The purpose of this study was to determine the effects of heat appli˗ cation on the immune activities of the human body. To exam, further˗ more, the immune effect from the healthy volunteer(male:15, female:15) by monitoring changes of immune substances such as various leukocytes[total white blood cell(WBC), eosinophil, neutrophil, basophil, monocyte, and lymphocyte], a comparative study with warm water immersion(40.8±0.3℃) and infrared(250W) was carried out. The plasma analysis showed that the count of white blood cell, eosinophil, and neutrophil were elevated in warm water immersion- or infrared˗ stimulated group compared with control group. However, the count of basophil was decreased in both warm water immersion- and infrared-stimulated group than control group. Therefore, these results suggest that the thermostimulation improved immune activity.

본 연구의 목적은 높이맵 기반의 액체 시뮬레이션을 이용하여 인간 친화적으로 표현하기 위한 감성 컨텐츠 제작 에 있다. 액체를 인간 친화적으로 표현하는 기존 연구들은 액체 표면을 표현하는데 몇 가지 문제점이 있다. 이러한 문 제를 해결하기 위해 본 연구는 액체의 광학적 특징을 이용한 새로운 액체의 카툰 렌더링 기법을 제안한다. 본 연구 에서 제안하는 액체의 카툰 렌더링은 반사와 굴절을 독립적으로 표현한 채색 단계와 액체의 경계선과 파형선을 표현 하는 선화 단계를 거쳐 표현된다. 그 결과 인간 친화적으로 표현된 액체의 표면을 실시간으로 감성 컨텐츠로 제작할 수 있다.

Many studies propose that dysfunction of mitochondrial proton-translocating NADH-ubiquinone oxidoreductase (complex I) is associated with neurodegenerative disorders, such as Parkinson's disease and Huntington's disease. Mammalian mitochondrial proton-translocating NADH-quinone oxidoreductase (complex I) consists of at least 46 different subunits. In contrast, the NDI1 gene of Saccharomyces cerevisiae is a single subunit rotenone-insensitive NADH-quinone oxidoreductase that is located on the matrix side of the inner mitochondrial membrane. With a recombinant adeno-associated virus vector carrying the NDI1 gene (rAAV-NDI1) as the gene delivery method, we were able to attain high transduction efficiencies even in the human epithelial cervical cancer cells that are difficult to transfect by lipofection or calcium phosphate precipitation methods. Using a rAAV-NDI1, we demonstrated that the Ndi1 enzyme is successfully expressed in HeLa cells. The expressed Ndi1 enzyme was recognized to be localized in mitochondria by confocal immunofluorescence microscopic analyses and immunoblotting. Using digitonin-permeabilized cells, it was shown that the NADH oxidase activity of the NDI1-transduced HeLa cells were not affected by rotenone which is inhibitor of complex I, but was inhibited by flavone and antimycin A. The NDI1-transduced cells were able to grow in media containing rotenone. In contrast, control cells that did not receive the NDI1 gene failed to survive. In particular, in the NDI1-transduced cells, the yeast enzyme becomes integrated into the human respiratory chain. It is concluded that the NDI1 gene provides a potentially useful tool for gene therapy of mitochondrial diseases caused by complex I deficiency.

본 연구는 터널이 갖는 다양한 특성 가운데 운전자의 심리적 안정성과 쾌적성 등 경관을 구성하는 설계요인과 운전자의 감성적 요인간의 관계를 파악하여, 터널경관에 대한 평가방법을 제시하고, 이를 위하여 터널경관을 구성하는 물리적인 구성요소와 운전자의 감성인지 변화와의 관계를 파악하여 경관적 환경을 조성할 수 있는 방안을 제시하는데 연구의 목적이 있다. 터널경관에 대한 감성인지특성을 파악하기 위한 LISREL 모형을 구축한 결과 터널경관 영향요인은 터널경관 구성요소 조화에 의한 감성 이미지인 것으로 분석되었다. 이러한 감성이미지는 설계요인과 개인속성에 의하여 설명며, 설계요인의 영향이 개인속성의 영향보다 크게 나타났다. 개인 속성과 설계요인 모두의 관계는 (+)의 관계로 나타났고 "안정성"과 "변화성"간에는 서로 밀접한 상관관계가 있는 것으로 분석되었다. 이때, "안전성" 요인이 "변화성" 요인보다 경관인지도에 좀 더 영향을 미치는 것으로 분석되어, 경관 평가 시 운전자는 기본적으로 "안전성"을 중시하나 "변화성"에 대하여도 요구하고 있는 것으로 분석되었다.

This study examined the effects of red light generated from a light emitting diode (LED) upon proliferation and mitochondrial stress in human gingival fibroblasts (hGFs). Cells were exposed to LED-generated red light at a clinically relevant intensity and distance with a 610-630 nm wavelength for various times (0-48 min). At different exposure times, cells were processed for the analysis of succinate dehydrogenase (SDH) activity, proliferation, mitochondrial membrane potential (MMP) and cytotoxicity. Cell cycle progression was also investigated by flow cytometry after staining with propidium iodide. Red light exposure was found to inhibit SDH activity and DNA synthesis in hGFs in a time-dependent manner. Light exposure also reduced the MMP levels in these cells and this was closely associated with a G0/G1 arrest. In contrast, exposure of hGFs to red light for 48 min led to a dramatic loss of MMP with an attendant increase in cytotoxicity. These findings demonstrate that LED-generated red light may cause mitochondrial stress and growth inhibition in hGFs during tooth whitening therapy, depending on the length of the exposure.

Human mesenchymal stem cell (hMSCs) isolated from human adult bone marrow have self-renewal capacity and can differentiate into multiple cell types in vitro and in vivo. A number of studies have now demonstrated that MSCs can differentiate into various neuronal populations. Due to their autologous characteristics, replacement therapy using MSCs is considered to be safe and does not involve immunological complications. The basic helix-loop-helix (bHLH) transcription factor Olig2 is necessary for the specification of both oligodendrocytes and motor neurons during vertebrate embryogenesis. To develop an efficient method for inducing neuronal differentiation from MSCs, we attempted to optimize the culture conditions and combination with Olig2 gene overexpression. We observed neuron-like morphological changes in the hMSCs under these induction conditions and examined neuronal marker expression in these cells by RTPCR and immunocytochemistry. Our data demonstrate that the combination of Olig2 overexpression and neuron-specific conditioned medium facilitates the neuronal differentiation of hMSCs in vitro. These results will advance the development of an efficient stem cell-mediated cell therapy for human neurodegenerative diseases.

HCI 분야에서 사용성은 시스템의 객관적인 사용성에 초점을 둔 것에서 점차 사용자들이 시스템을 사용하면서 느끼는 주관적인 경험을 중시하는 개념으로 확장, 변화하고 있다. 오늘날 대부분의 사람들은 휴대전화를 소지하고 사용한다. 휴대전화와 같이 인간과의 상호작용 빈도가 높은 인터페이스에서 보다 긍정적인 사용자의 주관적 경험을 유발하는 것은 중요하다고 볼 수 있다. 본 연구에서는 감정을 표현하는 인터페이스가 인간에게 보다 긍정적인 사용자 경험을 유발할 것이라는 가설 하에 감정을 표현하는 인간 목소리를 통해 청각적 피드백을 제공하는 휴대전화 프로토타입(prototype)을 제작한 후, 감정을 표현하기에 적절하지 않은 기계음을 통해 청각피드백을 제공하는 휴대전화 조건과의 비교를 통해 어떠한 조건에서 사용자들이 보다 더 긍정적인 사용자 경험을 느끼는지, 어떠한 조건을 더 선호하는지에 대해 알아보았다. 구체적으로, 참가자들은 4가지 종류의 휴대전화 프로토타입(청각적 피드백이 없는 무음 조건, 사람의 목소리를 통해 청각적 피드백을 제시하는 조건, 기계음을 통해 청각적 피드백을 제시하는 조건, 기계음과 사람의 목소리를 모두 제공하는 혼합음 조건)을 경험한 후, 그에 대한 경험적 사용성(재미, 흥미, 불쾌감), 유희적 측면(HQ) 그리고 선호도를 평정하였다. 결과적으로, 사용자들은 사람의 목소리를 통해 정서를 표현하는 조건의 휴대전화에 대해 청각적 피드백을 제공하지 않는 휴대전화나 기계음을 통해 청각적 피드백을 제시하는 휴대전화와 비교해서 상대적으로 높은 지각적 재미와 유희(HQ)를 느끼는 것으로 드러났다. 하지만 선호도는 다른 조건에 비해 낮은 수준의 평정치를 보이는 것으로 나타났다.