Stearoyl-CoA desaturase (SCD1) gene plays important role in fatty acid composition. In order to find marker-assisted selection (MAS) for improving the economic trait, this study was performed to identify the 878T>C single nucleotide polymorphism (SNP) on SCD1 gene. Three genotypes (CC, CT, and TT) were detected in 878T>C SNP of SCD1 gene from 103 Hanwoo population by polymorphism chain reaction-restriction fragment length polymorphism (PCR-RFLP) and economic traits were analyzed by general linear model. The frequency of allele C and T was 0.534 and 0.466, also the genotype frequency of CC, CT and TT was 0.252, 0.563, and 0.184, respectively in the Hanwoo population. The TT genotype of SCD1 gene showed a significantly higher measures (p<0.05) of carcass weight (CW) than CT, CC genotype. No significant association was detected between genotype and other economic trait (marbling, backfat thickness, and longissimus muscle area) in this study. The results revealed that SCD1 gene 878T>C SNP could be useful for effective MAS to increase the economic quality in Hanwoo population.

This study was carried out to investigate artificial insemination (AI) failure status and frozen semen characteristics in Korean proven bulls‘ number (KPN) semen used for AI of Hanwoo cows in Gangwon East region (Gangneung, Donghae, Taebaek, Samcheok, Sokcho, Yangyang, Goseong). Among semen used for AI, AI failure rate showed lowest at KPN506 (27.6%), whereas highest at KPN593 (77.2%). Correlations of AI failure in between Korean proven bulls semen and cows was 0.2941, which means that AI failure rate of Korean proven bulls semen may have respectable effect on reproduction of Hanwoo cow. In addition, present study was conducted to investigate spermatozoal viability rate, ruptured acrosome rate and active mitochondria in frozen Korean proven bulls semen with flow cytometry. The semen of KPN593 showed significantly (p<0.05) higher viability rate in KPN593 (30.49%) than that in KPN637 (37.34%). Furthermore, percentage of ruptured acrosome was lower in KPN637 as 21.37% than in KPN637 (21.37%), but it was not statistically significant. In conclusion, these results indicate that choice of Korean proven bulls semen may correlate positively with conception rate in Hanwoo cow. Therefore, KPN with high AI failure rate might be avoid to increase conception rate and characteristics of frozen semen might be evaluated before its use for AI.

Myopalladin (MYPN) is an important expression gene associated with regulation of Z-line structure in muscle and maintains sarcomeric integrity. In this study, we investigated the association between MYPN A1795G SNP (single nucleotide polymorphism) and carcass traits (LMA, longissimus muscle area; CW, carcass weight; BF, backfat thickness; MS, marbling score) in Korean cattle. The MYPN A1795G SNP was genotyped in 212 steers and analyzed the associations with carcass traits by PCR-RFLP (Restriction fragment length polymorphism) method. The allele frequencies were 0.566 for G allele and 0.434 for A allele. And the genotype frequencies of GG, GA, and AA genotype were 32.1%, 49%, and 18.9%, respectively. Association analysis indicated that the A1795G SNP of MYPN gene showed a significant association with LMA (p<0.05). The steers with GG genotype had higher LMA than those with the genotypes AA. But no significant associations were observed in other carcass traits (CW, BF, MS). The steers with the GG genotype showed higher CW and BF than those with the genotypes AA and GA. These results suggest that the A1795G SNP of the MYPN gene is associated with LMA and may be useful for candidate marker-assisted selection to increase the levels of LMA in Korean cattle.

The aim of this study was to analyze the reproduction and breeding status of 1655 Hanwoo (Korean cattle) cows raised in Gangwon East area (Gangneung, Taebaek, Donghae, Yangyang, Samcheok, Sokcho, and Goseong). The average age in months and the parity of Hanwoo was 43.6 and 1.7 respectively. Registration status of the cows were 42.0% (pedigree), 30.2% advanced), 20.0% (fundamental), and 4.0% (non-registry). Korean proven bull's number (KPN) 517 and KPN 588 were preferred 25.8%, 16.2% respectively for artificial insemination (AI) of the cows. Appearance rate of over 1st meat quality grade in the offsprings obtained from AI of top 6 KPNs was 90% which was above national average (slaughters only 77.81%, total 63.12). Results indicated genetic influence the KPN for meat quality grade of their offspring. Strong correleation between AI failure rate of mother cows and their offspring cows has been observed. KPN information, meat quality grade, and AI failure percentage would be quite useful factors to understand their relationship in further improvement of Hanwoo in Gangwon East area.

장대터널은 터널이 갖는 고유 특성으로 인하여 운전자의 주행안정성이 저하되고 사고위험성이 높기 때문에 신규 터널 설계시 사전에 충분한 검토가 필요하다. 특히 터널 주행시 나타나는 안정적인 속도유지 곤란과 졸음운전 가능성의 증가, 사고발생 시 높은 치사율 및 운전자가 경험하는 주관적인 불안감 등으로 인하여 설계 단계부터 시공에 이르기까지 교통심리학 및 인간공학적인 측면에서 숙고가 요구된다. 본 연구에서는 차량시뮬레이터를 이용하여 10km 이상의 장대터널(고속국도 60호선 춘천~양양간 건설공사 제14공구 내인제터널) 기본 설계안에 대한 운전자의 주행안정성을 평가한 후, 터널주행에 따른 문제점들을 파악하여 사고위험성이 높을 것으로 추정되는 위험구간 및 개선방안 등을 제시하였다. 실험 결과, 양양 및 춘천 방면 모두 도로선형이 바뀌는 변곡점을 전후하여 주행안정성이 저하되고 운전부하가 높아 위험구간으로 드러났다. 마지막으로 본 연구의 제한점 및 시사점에 대해 논의하였다.

In this paper, double texturization of multi crystalline silicon solar cells was studied with laser and reactive ion etching (RIE). In the case of multi crystalline silicon wafers, chemical etching has problems in producing a uniform surface texture. Thus various etching methods such as laser and dry texturization have been studied for multi crystalline silicon wafers. In this study, laser texturization with an Nd:YVO4 green laser was performed first to get the proper hole spacing and 300μm was found to be the most proper value. Laser texturization on crystalline silicon wafers was followed by damage removal in acid solution and RIE to achieve double texturization. This study showed that double texturization on multi crystalline silicon wafers with laser firing and RIE resulted in lower reflectance, higher quantum yield and better efficiency than that process without RIE. However, RIE formed sharp structures on the silicon wafer surfaces, which resulted in 0.8% decrease of fill factor at solar cell characterization. While chemical etching makes it difficult to obtain a uniform surface texture for multi crystalline silicon solar cells, the process of double texturization with laser and RIE yields a uniform surface structure, diminished reflectance, and improved efficiency. This finding lays the foundation for the study of low-cost, high efficiency multi crystalline silicon solar cells.

There are four (yellow, brindle, black, black in Jeju) kinds of native cattle in Korea. But only twelve hundred Korean brindle cattle (KBC) are remaining in limited areas of Korea and the genetic lineage, diversity, polymorphisms of KBC has not been identified. To analysis genetic polymorphism of KBC, 33 KBC were characterized using 11 microsatellite markers. Size of microsatellite marker was decided using Gene Mapper software after analysis ABI 3130XL. The average of allele numbers of KBC was 6.7 in this study, but that of Hanwoo was 10.0 in our previous report. The observed and expected heterozygosities of KBC were 0.719 and 0.738 but those of Hanwoo were 0.751 and 0.760 in our previous report. Also polymorphism information content (PIC) values were average 0.690 in KBC but 0.725 in Hanwoo. These results demonstrate that genetic polymorphism of KBC have decreased because the population was limited.

In this study, genotyping was executed by using 11 microsatellite markers (BM1824, BM2113, ETH10, ETH225, ETH3, INRA23, SPS115, TGLA122, TGLA227, TGLA53, and TGLA126) for diversity of 214 Hanwoo cows in Hoengseong area. Each marker's size and number of allele, observed heterozygosity, expected total heterozygosity, and polymorphism information content were analyzed by 11 Microsatellite marker. The average of size range was detected from 150.9 to 174.9 in Hanwoo cows of Hoengseong. The number of average allele was 10.0 that is similar to the average of Kangwon Hanwoo, which is known as 10.5 in the previous report. The average were 0.751 for observed heterozygosity, 0.760 for expected total heterozygosity, 0.725 for polymorphism information content, respectively. These results were similar to previous studies in Kangwon Hanwoo, National Hanwoo and Korean Proven Bulls. This study is expected to contribute for genetic improvement of Hanwoo cows in Hoengseong as a popular brand.

법적 보호종인 붉은점모시나비의 복원을 위한 모델 시스템 개발을 위해 붉은점모시나비와 생리･생태가 유사한 모시나비의 연중 계대사육법을 확립하고자 이 연구를 수행하였다. 모시나비의 알의 휴면타파를 위해 생리적 특성을 조사한 결과, 알의 호흡량은 산란 후 9일째부터 증가하기 시작하여 전-유충이 형성되는 14～15일째에 최대치를 나타내고 그 후 호흡량은 급격히 감소하며 20일째 이후 거의 변화가 없었다. 또한 알의 경과일수에 따른 단백질 패턴도 휴면 전(산란 후 14～18일째)과 휴면 후(산란 후 20, 25일째)가 서로 다름을 확인하였다. 이상의 결과를 토대로 저온처리에 의한 휴면타파 실험 결과, 저온처리 시기는 산란 후 전-유충이 형성되는 14일째보다는 17일, 18일째에 처리했을 때 부화율이 90%이상으로 높았다. 저온처리 기간은 90일이 소요되며, 온도는 5℃보다 2.5℃에서 부화율이 20%에서 25%로 다소 높아졌다. 부화된 유충은 산괴불주머니를 기주로 실내에서 사육한 결과, 15℃에서는 사육이 불가능하며, 25℃에서는 유충기간은 18.6일로 1령은 4일, 2령은 3.4일, 3령은 3.1일, 4령은 3.1일, 5령은 4.4일이었다. 용기간은 8.9일이었으며 용화율이 98%, 우화율은 90%이었다. 산란성 조사에서는 평균 산란수는 56개이었으며 최대 산란수는 93개이었다.