캐비어에 대한 수요가 증가하면서 캐비어 종류의 진위 여부를 확인 할 수 있는 분석법 마련이 필요해졌다. 본 연 구에서는 캐비어 종류을 나누는 철갑상어 종 특이 KASP 마커를 개발하였고 모니터링을 통해 국내 유통중인 불법 캐비어 제품을 확인하고자 하였다. 본 연구에서는 16S ribosomal RNA gene, cytochrome b gene, cytochrome c oxidase subunit I gene, cytochrome c oxidase subunit II gene, NADH dehydrogenase subunit 5 gene에서 철갑상어 종 특이적인 SNP를 선정하고 이를 표적하는 KASP 마커 11종을 개발하였다. 개발한 KASP 마커를 이용하여 한국 의 온라인 마켓에서 유통중인 캐비어 10종을 모니터링 분 석한 결과 2개의 제품에서 제품에 표기된 철갑상어 종과 다른 것으로 확인하였으며 두 제품 모두 실제보다 더 비 싼 캐비어로 둔갑하여 판매한 것으로 확인되었다. 본 연 구를 통해 제작한 KASP 분석방법으로 유통되는 캐비어 종류 분류가 가능하였고 모니터링을 통해 국내 유통되고 있는 불법 캐비어 제품도 확인하였다. 이에 따라 본 연구 에서 개발한 SNP기반 KASP 분석법으로 불법 캐비어 제 품 유통 근절에 기여 할 수 있을 것으로 기대한다.

The purpose of this study is to analyze the correlation between ecotoxicity and water quality items using Daphnia magna in public sewage treatment plant process and to obtain operational data to control ecotoxicity through research on removal efficiency. The average value of ecotoxicity was 1.39 TU in the influent, 1.50 TU in the grit chamber, and 0.84 TU in the primary settling tank and it was found that most organic matters, nitrogen, and phosphorus were removed through biological treatment in the bioreactor. Using Pearson’s correlation analysis, the positive correlation was confirmed in the order of ecotoxicity and water quality items TOC, BOD, T-N, NH3-N, SS, EC, and Cu. As a result of conducting a multilinear regression analysis with items representing positive correlation as independent variables, the regression model was found to be statistically significant, and the explanatory power of the regression model was about 81.6%. TOC was found to have a significant effect on ecotoxicity with B=0.009 (p<.001) and Cu with B=16.670 (p<.001), and since the B sign is positive (+), an increase of 1 in TOC increases the value of ecotoxicity by 0.009 and an increase in Cu by 1 increases the value of ecotoxicity by 16.670. TOC (β=0.789, p<.001) and Cu (β=0.209, p<.001) were found to have a significant positive effect on ecotoxicity. TOC and Cu have a great effect on ecotoxicity in the sewage treatment plant process, and it is judged that TOC and Cu should be considered preferentially and controlled in order to efficiently control ecotoxicity.

모기류는 흡혈을 통해 원충, 바이러스, 사상충 등 다양한 병원체를 보유하며 말라리아, 일본뇌염, 웨스트나일열, 뎅기열 등을 사람에게 매 개하는 위해성이 있는 위생해충이다. 이번 연구에서는 해외유입 모기류 감시를 위해 경상남도 고성군에 스마트 고공포집기를 설치하여 2022년 부터 2023년까지 모기류들을 모니터링하였다. 조사기간 동안, 총 3속 5종 43개체가 채집되었으며, 이중 삼일열말라리아를 매개하는 벨렌얼룩날 개모기(Anopheles belenrae)를 경남 지역에서 처음으로 발생함을 확인하였다. 본 연구에서는 스마트 고공포집기를 통해 해외에서 유입가능한 모 기류에 대한 실시간 모니터링이 가능함을 확인하였다.

This study was conducted to find a way to improve quality by observing changes in quality and microbial communities according to whether corn silage was treated with additives and the storage period, and to utilize them as basic research results. The experimental design was performed by 2˟4 factor desigh, and the untreated (CON), and the additive inoculated (ADD) silage were stored and fermented for 30 (TH), 60 (ST), 90 (NT), and 120 (OHT) days, with each condition repeated 3 times. There was no change in the nutrient content of corn silage according to additive treatment and storage period (p>0.05). However, the change in DM and the increase in the relative proportions of lactic acid content and Lactobacillales according to the storage period (p<0.05) indicate that continuous fermentation progressed until OHT days of fermentation. Enterobacterales (33.0%), Flavobacteriales (14.4%), Sphingobacteriales (12.7%), Burkholderiales (9.28%) and Pseudomonadales (6.18%) dominated before fermentation of corn silage, but after fermentation, the diversity of microorganisms decreased sharply due to the dominance of Lactobacillales (69.4%) and Bacillales (11.5%), Eubacteriales (7.59%). Therefore, silage maintained good fermentation quality with or without microbial additives throughout all fermentation periods, but considering the persistence of fermentation even in long-term storage and the aerobic stability, it would be advantageous to use microbial additives.

The experiment was conducted to determine the changes in seed productivity of Italian ryegrass (Lolium multiflorum Lam.) according to nitrogen fertilization levels in the southern region of Korea. Italian ryegrass (IRG) variety 'Green Call' was sown in the fall of 2021 in Jinju, Gyeongsangnam-do. The experiment consisted of three nitrogen fertilizer levels (100, 120, and 140 N kg/ha) with three replications using a randomized complete block design. Harvesting was done approximately 30 days after heading on May 18th. There was no difference in heading date among treatments, which occurred on April 18th. The longest IRG was observed in the 140 N kg/ha treatment, but there was no significant difference. No significant differences were observed in lodging, disease resistance, and cold tolerance among treatments, but lodging was severe in all treatments. The length of the spike averaged 44.95 cm, with no difference among treatments, and the number of seeds per spike was highest in the 120 N kg/ha treatment. Seed yield increased with increasing nitrogen fertilizer levels, averaging 3,707 kg/ha (as-fed basis). DM content of seed and straw averaged 76.95% and 62.19%, respectively, with no significant differences among treatments. The remaining straw after harvesting averaged 6,525 kg/ha on a dry matter basis, with the highest value observed in the 140 N kg/ha treatment. Overall, considering the results, the optimal nitrogen fertilizer application rate for seed production of Italian ryegrass in the southern region when sown in autumn was found to be 120 N kg/ha.

이 연구는 금융교육 표준안 활용 경험이 있는 초등교사의 금융교육 표준안 활용의 어려움을 기술 및 분석한 후 금융교육 표준안 개정에 대한 시사점을 제언하고자 수행되었다. 연구를 수행하기 위해 A 연구회의 금융교육 교육과정 개발에 참여한 초등교사 14명을 연구 참여자 로 선정하였고, 심층면담을 통해 자료를 수집한 후 질적 분석을 수행하였다. 분석을 수행한 결과 교사들이 경험한 금융교육 표준안 사용의 어려움을 네 가지 범주로 분류하였고 이는 다음과 같다. 첫째, 교사들은 초등학생의 수준이 고려되지 않은 금융교육 표준안의 단일한 성취기준에 의해 어려움을 겪었다. 둘째, 교사들은 성취기준 진술 방식으로 인해 자율성이 제한되는 경험을 했다. 셋째, 교사들은 금융교육 표준안의 일부 교수학습 방법 및 유의사항 에서 실질적인 도움을 받지 못해 어려움을 겪었다. 넷째, 교사들은 수업을 설계하면서 성취 기준 내용의 중첩으로 인해 어려움을 겪었다. 이를 토대로 본 연구에서는 향후 금융교육 표준안 개정에 대한 시사점을 제언하였다.

We analyzed risk factors of coastal gillnet fishers during fishing process and considered work safety measures to reduce safety accidents during fishing using a UWB (ultra wideband) based positioning system. The static position accuracy of the UWB based positioning system was 45 cm. When entering a port, there is a risk of falling overboard. When casting a net, there was a risk of falling overboard due to being hit by fishing gear or guards, or getting caught in a buoy line or sinker line. When hauling a net, there is a risk of getting caught between fishing gear and net hauler, and the risk of musculoskeletal disorders due to repetitive work over a long period of time. Most safety accidents during work on fishing boats are blamed on human errors of the fisher and skipper, but safety accidents occur due to a mixture of mechanical and equipment factors, work and environmental factors, and management factors in addition to human errors. Therefore, the 4E were presented as countermeasures against the 4M, which are causes of safety accidents, and the proposed measures were used to identify risk factors for operation process, comply with work safety rules, and ensure the wearing of personal protective equipments. We need to reduce safety accidents during work by making it part of our daily routine. These research results can be used in the future for optimal placement of fishing gear and fishing nets in other coastal industries where safety accidents occur frequently.

Recently, domestic fishing production of Japanese horse mackerel has been continuously decreasing. To achieve sustainable fishing of this species, it is essential to acquire its target strength (TS) for accurate biomass estimation and to study its ecological characteristics. To date, there has been no TS research using a broadband echosounder targeting Japanese horse mackerel. In this study, for the first time, we synchronized an underwater camera with a broadband frequency (nominal center frequency of 200 kHz, range: 160-260 kHz) to measure the TS according to the body size (16.8-35.5 cm) and swimming angle of the species. The relationship between Japanese horse mackerel length and body weight showed a general tendency for body weight to increase as length increased. The pattern of the frequency spectra (average values) by body length exhibited a similar trend regardless of body length, with no significant fluctuations in frequency observed. The lowest TS value was observed at 243 kHz while the highest TS values were recorded at 180 and 257.5 kHz. The frequency spectra for the swimming angles appeared to be flat at angles of –5, 0, 30, 60, 75, and 80° while detecting more general trends of frequency spectra for swimming angle proved challenging. The results of this study can serve as fundamental data for Japanese horse mackerel biomass estimation and ecological research.

This study investigated the species composition and community structure of aquatic organisms captured using coastal beam trawling in Gomso Bay, Jeollabuk-do, from January to December 2022. Throughout the experimental period, a total of 20,246 individuals belonging to 94 marine species were captured, with a combined biomass of 602,828 g. Fish exhibited the highest abundance, comprising 56 species, followed by crustacea (21 species), bivalvia (8 species), cephalopoda (5 species), gastropoda (3 species), and holothuroidea (1 species). The dominant species was Leiognathus nuchalis, constituting 14.0% of the total individuals, followed by Portunus trituberculatus at 12.1%, Oratosquilla oratoria at 10.4%, Crangon hakodatei at 9.9%, and Metapenaeus joyneri at 7.9%. The diversity index ranged from 1.72 to 2.55, with the lowest diversity observed in March and the highest in July. Cluster analysis based on species composition of the 27 most common species showed that aquatic organisms were divided into three groups: spring and summer organisms (Group A) and summer organisms (Group B) and autumn and winter organisms (Group C).