산화적 스트레스는 세포 및 조직 손상을 통해 피부의 탄력 및 보습 기능 저하, 피부 노화 촉진 을 비롯한 다양한 피부질환을 일으킨다. 본 연구의 목적은 인간 피부각질세포 (HaCaT keratinocyte)에서 산화적 스트레스에 대한 붉은 토끼풀 추출물의 효능을 검토하여, 피부에 효과적으로 사용할 수 있는 기능 성 소재로서의 활용 여부를 확인하고자 하였다. 본 연구에서는 붉은 토끼풀 추출물이 인간 피부각질세포에 서 산화적 스트레스에 따른 세포사를 억제시키는 것을 확인하여, 이를 조절하는 보호기전을 규명하였다. 이는 붉은 토끼풀 추출물이 Caspase-3 비활성, 세포사 촉진단백질 Bax 발현 억제, 세포생존 촉진단백질 Bcl-2 발현 증가 및 MAPK 신호전달계 단백질의 인산화 억제를 통해 H2O2에 의해 유도된 산화적 스트레 스를 보호할 수 있다는 것을 확인하였다. 따라서 붉은 토끼풀 추출물은 피부의 산화적 손상을 감소시키는 유용한 소재로 평가되며, 이는 피부보호 및 미용을 위한 다양한 제품 및 산업에 활용 가능성이 높은 것으로 판단된다.

This study was carried out to determine the sprouting period of early and mid-season varieties, which includes ‘Atlantic’, ‘Chubaek’, and ‘Superior’, during the summer storage period in a semi-underground warehouse without cooling system. And also it was investigated the effect of chlorpropham [Propan-2-yl N-(3-chlorophenyl)carbamate, CIPC] treatment on the sprouting inhibition for the varieties. This study was conducted to figure out a sprout inhibitory effect when CIPC was applied to 1kg of the potato tubers at concentrations of 10 mg and 20 mg which are lower than the treatment concentrations of ca 30 mg prescribed by the positive list system (PLS). The internal temperature of the warehouse used in this experiment was lowered by 5°C or more than the outside temperature. The difference between the lowest and highest temperature during the experiment throughout the day was 5°C. It showed the effect of reducing to 1/2 of the difference in outdoor temperature. As for the sprouting of potatoes, the extremely early variety ‘Chubaek’ sprouts appeared at the 6th week of storage of control and it was the fastest sprouting potato among the control groups of the varieties. Sprouting began to appear in the Superior at the 6th week of storage, while the ‘Atlantic’ sprouted at the 8th week of storage. The appearance of sprouts was suppressed in all treatment groups of ‘Atlantic’ and ‘Superior’ varieties in CIPC treatments. Sprouts were observed in all treatment groups of ‘Chubaek’ after the 7th week, but the elongations of the sprouts in tubers were completely inhibited until the 8th week of storage. ‘Atlantic’ and ‘Superior’ seemed to have a sprouting inhibitory effect even with a low CIPC concentration of 10 mg·kg-1, with the exception of extremely early variety ‘Chubaek’ that breaks out of the dormancy quickly. Although weight loss occurred continuously during storage, it was minor loss of 0.7-1.6%. There was no consistent trend for changes of the loss in the varieties and CIPC treatments. Most common pathological disorder was the dry rot during the experiment, but only few were affected. The use of the tubers treated at 18°C and 90% RH for 10 days and the rack of refrigeration system which lead to lack of convection seemed to have suppressed the spread of pathogens.

Potato dry rot is one of the potato storage diseases caused by Fusarium species and is a representative pathological disorder that induced post-harvest loss during storage. Chlorpropham treatment for sprouting inhibition is mainly used for room temperature storage of potatoes for processing. In this study, the inhibitory effect of chlorpropham on Fusarium-induced dry rot of potato ‘Dano’. To investigate the mycelial growth rate of the dry rot fungus (Fusarium solani Appel & Wollenw), mycelial growth was investigated in a chlorpropham (5.0, 50.4, 503.8, and 5,038 ppm) and prochloraz (0.1, 1.0, 10.0, and 100.0 ppm) medium containing F. oxysporum mycelia. Mycelia were more inhibited as the concentration of chlorpropham and prochloraz increased during incubation at 20°C, and the inhibition rate was 98.2% and 100% when treated with 503.8 ppm of chlorpropham and 10ppm of prochloraz in 14 days, respectively. Potato Dano tubers inoculated with F. oxysporum were dipped in chlorpropham (5.0, 50.4, and 503.8 ppm) and prochloraz (100 ppm) to investigate the effect of preventing dry rot during cold storage at 20°C and 4°C in vivo. The disease diameter of potatoes stored at room temperature (about 20°C) was reduced to 13.0 mm in the prochloraz 100 ppm teatment, and 10.7 mm in the chlorpropham 50.4 ppm treatment compared to 13.7 mm in the control tuber at 70 days of storage. The disease progression in all treatments including control was similar with no statistically significant difference at 4°C air temperature. From the results of this study, it is considered that treatment with 50.4 ppm of chlorpropham after harvest will be useful for suppressing dry rot of stored potatoes.

With rising concerns about pesticide spray drift by aerial application, this study attempt to evaluate aerodynamic property and collection efficiency of spray drift according to the leaf area index (LAI) of crop for preventing undesirable pesticide contamination by the spray-drift tunnel experiment. The collection efficiency of the plant with ‘Low’ LAI was measured at 16.13% at a wind speed of 1 m·s-1. As the wind speed increased to 2 m·s-1, the collection efficiency of plant with the same LAI level increased 1.80 times higher to 29.06%. For the ‘Medium’ level LAI, the collection efficiency was 24.42% and 43.06% at wind speed of 1 m·s-1 and 2 m·s-1, respectively. For the ‘High’ level LAI, it also increased 1.24 times higher as the wind speed increased. The measured results indicated that the collection of spray droplets by leaves were increased with LAI and wind speed. This also implied that dense leaves would have more advantages for preventing the drift of airborne spray droplets. Aerodynamic properties also tended to increase as the LAI increased, and the regression analysis of quadric equation and power law equation showed high explanatory of 0.96－0.99.

수많은 함정용 채프들은 폭발에 의해 확산되어 채프운을 형성하며, 채프운은 허위 레이더 반사 단면적을 생성하여 적의 레이더를 기만한다. 본 논문에서는 전산유체역학-이산요소법 단방향 연동 기법을 기반으로 공기 중에 분포하는 함정용 채프운의 시공간 분포 를 해석하는 수치적 프레임워크를 구축하고 바람의 방향과 속도, 채프 카트리지의 초기 각도와 폭발 압력이 채프운 분포에 미치는 영 향을 분석하였다. 채프운의 확산은 폭발에 의한 방사형 확산, 난류와 충돌에 의한 전 방향 확산, 낙하 속도 차이에 의한 중력 방향 확산 과 같이 세 단계로 구분되는 것을 확인하였다. 바람은 채프운의 평균 위치를 이동시켰으며, 항력에 의한 확산 효과는 나타나지 않았다. 카트리지 초기 각도에 따라 폭발에 의한 방사형 확산 방향이 달라졌으며, 각도가 지면과 수직에 가까울수록 더 넓게 확산되었다. 폭발 압력이 증가할수록 채프운은 더 넓게 확산되었으나 중력 방향으로는 분포 차이가 작았다.

This study was conducted to apply with an air duct for the cooling and a utilizing cultivating method that uses the fruiting node and the defoliation to the high-temperature vertical and hydroponic cultivation of the oriental melon. The lower fruiting node (LF) was to remove all third vines generated from 5 nodes of a secondary vine. The higher fruiting node (HF) was fruiting on the third vine generated from a first node of the third vine. The direction of the stem string; upward (UW), downward (DW). Four treatment conditions were applied with the LF-UW, LF-DW, HF-UW (control), and HF-DW. The leaf age of melon leaves was measured for photosynthesis at 3 days intervals, and the fruit characteristic was conducted on 79 fruits in each treatment. The photosynthesis rate steadily increased after leaf development, reaching 20.8 μmol CO2·m-2·s-1 on the 10 days, gradually increasing to 21.3 μmol CO2·m-2·s-1 on the 19 days, and reaching 23.4 μmol CO2·m-2·s-1 on the 32 days. After that, it lowered to 16.8 μmol CO2·m-2·s-1 on the 38 days and dropped significantly to 7.6 μmol CO2·m-2·s-1 on the 47 days. As a result of the fruit characteristics by fruiting nodes, the treatments of the fruit length was 12.6-13.4 cm, respectively, which was significant, and the fruit width was 7.9- 8.6 cm, respectively, was not significant. The soluble content ranged from 12.9 to 15.7°Brix, and the significance of all treatments, and higher than of LF-DW and HF-UW. The photosynthesis rate of melon leaves was good until 32 days after leaf development, but after that, the rate decreased. As for fruit quality, it was conformed that melons can be cultivated at the LF because the fruit enlargement and soluble content dose not decrease even when set at the LF. Results indicated that those can be used for LF and defoliation in the development of vertical and hydroponic cultivation method in high-temperature season.

Since delivery food has become a new dietary culture, this study examines consumer awareness through big data analysis. We present the direction of delivery food for healthy eating culture and identify the current state of consumer awareness. Resources for big data analysis were mainly articles written by consumers on various websites; the collection period was divided into before and after COVID-19. Results of the big data analysis revealed that before COVID-19, delivery food was recognized as a limited product as a meal concept, but after COVID-19, it was recognized as a new shopping list and a new product for home parties. This study concludes by suggesting a new direction for healthy eating culture.

Sarcomatoid carcinoma is rarely diagnosed as gallbladder cancer. Its aggressive nature, due to the characteristics of both sarcoma and carcinoma, results in a poor prognosis. We report a case of gallbladder sarcomatoid carcinoma in an 82-year-old male who was referred to our hospital for evaluation of gallbladder cancer observed on abdominopelvic computed tomography. The characteristics of the cancer were not confirmed after several imaging modalities. The surgically resected tumor was positive for both cytokeratin and vimentin as revealed via immunohistochemical staining, and a sarcomatoid carcinoma was finally diagnosed. The role of chemotherapy has not yet been identified. Therefore, radiation therapy is planned to reduce the risk of recurrence.

목적 : 본 연구는 무릎관절 치환술 환자를 위한 작업치료 임상 가이드라인을 체계적으로 개발하고자 하였다. 전 세계적으로 무릎관절 치환술을 받은 환자들의 회복과 지역사회 복귀에 있어 작업치료의 역할이 매우 중요하다는 문헌이 보고되고 있으나, 우리나라에서는 관절 치환술 환자를 위한 작업치료가 거의 제공되지 않는다는 현실이 있다. 본 연구에서는 무릎관절 치환술 환자를 위한 근거기반 작업치료 중재를 확인하고 이것을 가이드라인을 통해 제안하고자 한다. 연구방법 : 가이드라인 개발 방법은 영국 작업치료사협회의 ｢Practice guideline development manual(4th edition)｣에서 소개한 절차를 따랐다. Patient/Population, Intervention, Comparison and Outcomes(PICO) 임상 질문 설정은 이해관계자의 의견을 수렴하여 한국의 보건·의료제도를 고려하여 설정하고 이를 바탕으로 체계적 고찰을 진행하였다. 데이터베이스에서 총 7,241건의 논문을 추출하였으며 논문의 질 평가 및 비평적 읽기를 통해 최종 66편의 근거 논문을 선정하였다. 논문의 중재를 바탕으로 가이드라인 내용을 구성하고 권고 강도는 Grading of Recommendations, Assessment, Development, and Evaluations(GRADE) approach 체계를 활용하여 [권고]와 [제안]으로 구분하였다. 결과 : 무릎관절 치환술 환자를 위한 작업치료 임상 가이드라인 권고 내용은 ‘환자 중심’과 ‘서비스 제공 중심’으로 범주화하였다. ‘환자 중심’범주에는 ‘일상생활 활동기능 향상’, ‘정신건강의 안정(불안, 우울 등)’, ‘환자 만족도 증가’, ‘지역사회복귀 향상’을, ‘서비스 제공 중심’범주에는 ‘작업치료사를 포함한 다학제 재활팀 구성’과 ‘작업치료 를 통한 병원 입원 기간 단축’을 위한 내용을 포함하였다. 결론 : 무릎관절 치환술 환자를 위한 작업치료 임상 가이드라인은 수술환자들의 안정적인 지역사회 복귀와 입원 기간 단축에 기여할 것이다. 또한 정형외과 작업치료사 역량 강화를 위한 교육자료로 활용될 수 있으며 의료비 용 감소를 위한 정책 마련에 근거 자료가 될 것이다.

The predator-prey interaction in freshwater ecosystems is a crucial area in the ecological study field and one of example to find such interaction is to investigate stomach contents. However, traditional method through visual inspection often induce misidentification, as it depends critically on intactness of physically visible data. In this study, we utilized Next-Generations Sequencing (NGS) technology to test the applicability stomach content analysis and overcome such limitation. NGS was applied to analyze the stomach contents of the Hemibarbus labeo, Tachysurus fulvidraco, and Plecoglossus altivelis collected in the lower part of Nakdong River. As a result, T. fulvidraco had a higher number of Animalia operational taxonomic units (OTUs) intake rate than H. labeo. At the same time, P. altivelis had higher number of Plantae OTUs intake rate than T. fulvidraco and higher Protozoa OTUs intake rate than H. labeo respectively. Therefore, NGS technology application enable to overcome traditional method’s limitation and discover hidden interspecific interaction which can further be used in appropriate habitat assessment.

목적 : 본 연구는 신체적 기능장애인을 대상으로 장애수용, 자아존중감, 우울, 인권이 삶의 만족도에 미치 는 영향을 알아보고자 하였다. 연구방법 : 한국장애인개발원의 장애인삶 2차 패널조사에 참여한 6,121명 중 신체기능 장애인 1,780명의 데이 터를 추출하여 일반적인 특성, 장애수용, 자아존중감, 우울, 인권 항목을 분석하였다. 자료는 기술 통계, 피어슨 상관분석, 다중회귀분석을 실시하여 분석하였다. 결과 : 신체기능 장애인의 장애수용, 자아존중감, 우울, 인권과 전반적인 삶의 만족도는 유의미한 상관관계 를 보였다. 우울감이 낮을수록, 장애수용과 자아존중감, 인권이 높을수록 삶의 만족도에 긍정적인 영향을 보였다. 장애수용, 자아존중감, 우울, 인권이 전반적인 삶의 만족도에 미치는 영향은 41%의 설명력을 보 였다. 결론 : 신체적 기능장애인의 긍정적인 삶의 만족도를 위하여 장애인 자신의 긍정적 장애수용, 지역사회의 심리정서적 지원과 함께 비장애인들의 장애인식에 따른 관점변화가 이루어질 필요가 있다.

Cyanobacterial resting cells, such as akinetes, are important seed cells for cyanobacteria’s early development and bloom. Due to their importance, various methods have been attempted to isolate resting cells present in the sediment. Ludox is a solution mainly used for cell separation in marine sediments, but finding an accurate method for use in freshwater is difficult. This study compared the two most commonly used Ludox methods (direct sediment treatment and sediment distilled water suspension treatment). Furthermore, we proposed a highly efficient method for isolating cyanobacterial resting cells and eDNA amplification from freshwater sediments. Most of the resting cells found in the sediment were akinete to the Nostocale and were similar to those of Dolichospermum, Cylindrospermum, and Aphanizomenon. Twenty times more akinetes were found in the conical tube column using the sediment that had no treatment than in the sample treated by suspending the sediment in distilled water. Akinete separated through Ludox were mainly spread over the upper and lower layers in the column rather than concentrated at a specific depth in the column layer. The mibC, Geo, and 16S rDNA genes were successfully amplified using the sediment directly in the sample. However, the amplification products of all genes were not found in the sample in which the sediment was suspended in distilled water. Therefore, 5 g to 10 g of sediment is used without pretreatment when isolating cyanobacterial resting cells from freshwater sediment. Cell isolation and gene amplification efficiency are high when four times the volume of Ludox is added. The Ludox treatment method presented in this study isolates cyanobacterial resting cells in freshwater sediment, and the same efficiency may not appear in other biotas. Therefore, to apply Ludox to the separation of other biotas, it is necessary to conduct a pre-experiment to determine the sediment pretreatment method and the water layer where the target organism exists.

목적 : 본 연구는 신체활동 참여와 도구적 일상생활활동(Instrumental Activities of Daily Living; IADL) 자립도 사이의 인지기능의 매개효과를 조사하였다. 연구방법 : 국민건강보험공단의 노인코호트 데이터베이스 자료를 활용하여 건강보험 및 의료급여 자격을 유지하고 있는 만 60세 이상의 노인 약 600만 명 중 신체활동 설문에 참여한 3,980명의 자료를 활용하 였다. 신체활동과 IADL 사이의 인지기능의 매개효과를 확인하기 위해 경로분석을 시행하였다. 연구결과 : 신체활동에 참여하는 노인의 평균 연령은 81.6세였으며, 여성이 72.74%였다. 인지기능의 부분매개 효과는 고강도 운동에서 68%로 IADL의 자립도에 가장 크게 나타났다(β = 0.07, 95% Confidence Interval; CI = 0.05, 0.09). 다음으로 중등도 운동은 66%(β = 0.08, 95% CI = 0.06, 0.10), 걷기 운동은 52%(β = 0.12, 95% CI = 0.11, 0.14) 순으로 나타났다. 신체활동과 IADL 자립도 사이의 직접효과는 걷기 운동에서 48%(β = 0.11, 95% CI = 0.09, 0.14)로 가장 크게 나타났으며 중등도 운동은 35%(β = 0.04, 95% CI = 0.02, 0.07), 고강도 운동은 32%(β = 0.03, 95% CI = 0.01, 0.06) 순으로 나타났다. 결론 : 본 연구를 통해 신체활동과 IADL의 관계에 인지기능의 매개효과가 있음을 확인할 수 있었다. 노인 의 건강하고 활동적인 노화를 위해 신체활동과 인지기능을 결합한 복합운동 프로그램이 고려되어야 한다.