Recently microplastic (MP) biofilm is being attracted as an important environmental issue because it can act as a pollutant carrier in aqueous system. Therefore, this study investigated the MP biofilm communities originated from freshwater. The results showed the bacterial community structure of MP biofilm was distinctively different from the freshwater regardless of biofilm-forming condition and MP type. For MP biofilm communities exposed to raw freshwater, Solimonas variicoloris-like microbe, Frigidibacter albus-like microbe, Nitrospirillum amazonense-like microbe, and Pseudochroococcus couteii-like microbe became abundant, while Acinetobacter johnsonii, Macellibacteroides fermentans, and Sedimentibacter acidaminivorans-like microbe were found as major bacteria for MP biofilm communities exposed to organic rich condition. The results of this study suggest that the unique freshwater biofilm community could be formed on the MP surface.

Ammonia (NH3) is a basic gas in the atmosphere and is known to play an important role in producing adverse health and environmental effects. Atmospheric NH3 causes stunted livestock growth, decreased visibility, and induces lung diseases when high concentrations occur. In addition, atmospheric NH3 reacts with acidic species (sulfuric acid, nitric acid, etc.) and produces secondary inorganic aerosol. In this study, the NH3 concentration and ventilation of Rooms 1 to 3 inside a sow facility were measured during the period from March 25 to May 31, 2021. It was difficult to conduct long-term field experiments at housing where pigs are raised. However, in order to improve the accuracy and reliability of the data, repeated experiments were conducted in three pig rooms in the same environment. The average concentration of NH3 in Rooms 1 to 3 was measured to be 7.6 ± 2.7 ppm, 8.2 ± 2.8 ppm, and 8.2 ± 2.7 ppm, respectively. The average internal temperatures were 21.0 oC, 21.2 °C, and 21.8 °C, and the internal humidity was 49.3%, 49.2%, and 49.2%, respectively. The ventilation per pig in Rooms 1 to 3 was measured as 60.4m3/hour∙pig, 62.5m3/hour∙pig, and 64.9m3/hour∙pig, respectively. At this time, NH3 emissions from Rooms 1 to 3 were found to be 6.9 ± 0.8 g/day∙pig, 7.9 ± 1.5 g/day∙pig, and 8.2 ± 1.3 g/day∙pig, respectively. As a result of the correlation analysis, the NH3 concentration was analyzed as producing a negative correlation between the ventilation (r=-0.73) and the internal temperature (r=-0.60) increase. Finally, as a result of calculating the national NH3 emission factor, the NH3 emission of one sow room in spring was 7.7 ± 1.4 g/day∙pig, and the NH3 emission of one year was 2.8 kg/ year∙pig.

Somatic cell nuclear transfer (SCNT) in pigs has been used as a very important tool to produce transgenic for the pharmaceutical protein, xenotransplantation, and disease model and basic research of cloned animals. However, the production efficiency of SCNT embryos is very low in pigs and miniature pigs. The type of donor cell is an important factor influencing the production efficiency of these cloned pigs. Here, we investigated the developmental efficiency of SCNT embryos to blastocysts and full term development using fetal fibroblasts (FF) and mesenchymal stem cells (MSCs) to identify a suitable cell type as donor cell. We isolated each MSCs and FF from the femoral region and fetus. Cultured donor cell was injected into matured embryos for cloning. After that, we transferred cloned embryos into surrogate mothers. In term of in vitro development, the SCNT embryos that used MSCs had significantly higher in cleavage rates than those of FF (81.5% vs. 72%) (p<0.05), but the blastocyst formation rates and apoptotic cell ratio was similar (15.1%, 6.18% vs. 20.8%, 9.32%). After embryo transferred to surrogates, nine and nineteen clone piglets were obtained from the MSCs and FF group, respectively, without significant differences in pregnancy and birth rate (50%, 40% vs. 52.3%, 45.4%) (p>0.05). Moreover, there was no significant difference in the corpus hemorrhagicum numbers of ovary, according to pregnancy, abortion, and delivery of surrogate mothers between MSCs and FF groups. Therefore, the MSCs and FF are useful donor cells for production of clone piglets through SCNT, and can be used as important basic data for improving the efficiency of production of transgenic clone pigs in the future.

Global warming has a major impact on the Earth’s precipitation and temperature fluctuations, and significantly affects the habitats and biodiversity of many species. Although the number of alien plants newly introduced in South Korea has recently increased due to the increasing frequency of international exchanges and climate change, studies on how climate change affects the distribution of these alien plants are lacking. This study predicts changes in the distribution of suitable habitats according to RCPs climate change scenarios using the current distribution of the invasive alien plant Conyza sumatrensis and bioclimatic variables. C. sumatrensis has a limited distribution in the southern part of South Korea. Isothermality (bio03), the max temperature of the warmest month (bio05), and the mean temperature of the driest quarter (bio09) were found to influence the distribution of C. sumatrensis. In the future, the suitable habitat for C. sumatrensis is projected to increase under RCP 4.5 and RCP 8.5 climate change scenarios. Changes in the distribution of alien plants can have a significant impact on the survival of native plants and cause ecosystem disturbance. Therefore, studies on changing distribution of invasive species according to climate change scenarios can provide useful information required to plan conservation strategies and restoration plans for various ecosystems.

Metal-organic frameworks (MOFs) are widely used in various fields because they make it easy to control porous structures according to combinations of metal ions and organic linkers. In addition, ZIF (zeolitic imidazolate framework), a type of MOF, is made up of transition metal ions such as Co2+ or Zn2+ and linkers such as imidazole or imidazole derivatives. ZIF- 67, composed of Co2+ and 2-methyl imidazole, exhibits both chemical stability and catalytic activity. Recently, due to increasing need for energy technology and carbon-neutral policies, catalysis applications have attracted tremendous research attention. Moreover, demand is increasing for material development in the electrocatalytic water splitting and metal-air battery fields; there is also a need for bifunctional catalysts capable of both oxidation/reduction reactions. This review summarizes recent progress of bifunctional catalysts for electrocatalytic water splitting and metal-air batteries using ZIF-67. In particular, the field is classified into areas of thermal decomposition, introduction of heterogeneous elements, and complex formation with carbonbased materials or polyacrylonitrile. This review also focuses on synthetic methods and performance evaluation.

농업기계는 다양한 환경과 작물을 대상으로 작업을 수행하기 때문에 농업기계의 설계와 개발은 실 작업 부하를 고려하여 수행되어야 한다. 따라서 본 연구에서는 식용 옥수수 수확기의 실 작업 부하를 계측하기 위해 식용 옥수수 수확 시험을 수행하였다. 또한, 계측된 부하 데이터를 이용하여 수확기 내 기어박스 요소들의 강도 및 수명을 평가하기 위한 부하 스펙트럼을 구축하였다. 부하 스펙트럼은 기어박스의 구성요소 중 기어와 베어링에 적용 가능한 하중 지속 분포와 기어와 베어링을 제외한 축, 하우징, 캐리어 등에 적용 가능한 부하 스펙트럼으로 나누어 구축하였다. 본 연구를 통해 구축된 하중 지속 분포 및 부하 스펙트럼은 식용 옥수수 수확기의 기어박스 강도 및 수명 평가뿐만 아니라, 기어박스의 설계 하중으로도 활용이 가능할 것으로 보인다.

N-nitrosodimethylamine (NDMA) is a potent carcinogen that is frequently detected nitrosamine from water chloramination. This study investigated the occurrence of NDMA and its potential precursor, ranitidine (RNT), in four wastewater treatment plants (WWTPs). Additionally, the effects of chloramination methods and oxidative pretreatment on the NDMA formation potential (FP) were assessed. Concentration levels of NDMA in the WWTPs waters ranged from 2.5 (detection limit) to 72.6 ng/L, while RNT values ranged from 1.32 to 186.9 ng/L. Further study indicated that the NDMA-FPs from chloraminated wastewaters varied between 36.2 and 227.8 ng/L. Nonetheless, chloramination methods and oxidative pretreatment significantly impacted the NDMA-FP levels. For example, breakpoint chlorination and stepwise chloramination promoted NDMA-FP when compared to preformed chloramination, which could be attributed to the formation of dichloramine and chlorine species. In contrast, prechlorination was found to effectively mitigate NDMA-FP, based on integrated ultraviolet (UV) irradiation. Notably, UV irradiation with free chlorine (UV/Cl2) or permanganate (UV/MnO4 -) reduced NDMA-FP by up to 70%. This study suggests that UV/MnO4 - and UV/Cl2 may be used as alternative mitigation strategies for reducing nitrosamine-FP in the water treatment process.

본 연구에서는 남자 실업팀 핸드볼 선수들을 대상으로 플라이휠 운동을 적용한 웨이트 트레이 닝 프로그램을 개발하여 상해예방과 경기력 향상을 위한 기초자료를 제공하는데 그 목적이 있다. 12주간 복합 플라이휠 운동 프로그램을 적용하여 신체조성, 체력요인, 무산소성파워, 등속성근기능에 미치는 영향 을 분석한 결과는 다음과 같다. 팔굽혀펴기는 그룹 간 유의한 차이가 나타났다. 체지방률, 무산소성 파워는 시기별 상호작용에서 유의한 차이가 나타났다. 제자리멀리뛰기, 제자리높이뛰기, 악력, 배근력, 무산소성평 균파워 그리고 등속성근기능검사는 좌굴근과 우굴근에서 시기별로 유의한 차이가 나타났다. 따라서 12주 간 플라이휠 운동을 활용한 웨이트 트레이닝 결과는 상해예방과 경기력 향상을 위한 기초자료로 제공할 수 있을 것으로 판단된다.

Airborne bacteria are expected to float in the mushroom cultivation house, as it is a special environment with high humidity and high temperatures. Their concentration and diversity in the indoor air of the cultivation house could effect the health of farmers and the quality of mushrooms. To examine whether microbiota of airborne bacteria change from year to year, we measured the indoor temperature, humidity, and airborne bacterial concentration from mushroom cultivation houses located in six regions in Korea from 2020 to 2021, and isolated and identified airborne bacteria. The surveyed data were compared and the bacterial diversity of the 1st year and the 2nd year were determined. Based on the average temperature and humidity data surveyed, it can be seen that the temperature and humidity environment in the cultivation houses is such that bacteria can easily reproduce. It was observed that the temperature inside the cultivation houses tends to be higher or lower depending on the season and correlates with the temperature outside the cultivation houses. In the first year survey, 32 species of 20 genera were identified, and in the second year survey, bacteria belonging to 29 species of 22 genera were identified. Among them, the most detected species were all species belonging to the genus Bacillus. There were only three species (Bacillus altitudinis, Brevibacterium frigoritolerans, and Staphylococcus epidermidis) that were continuously isolated in common. Our results showed that the species of floating bacteria greatly vary from year to year even for the same cultivation houses.

본 연구의 목적은 운전상황에서 운전자의 스트레스가 유발되었을 시, 다감각 자극이 운전자의 스트레스 저감 효과에 미치는 영향을 분석하였다. 본 연구에서는 운전상황의 운전자의 스트레스를 저감을 목적으로 조명과 사운드 및 향의 조합을 통한 다감각 자극을 제시함으로써, 스트레스 저감에 대한 효과를 확인하고자 하였다. 운전 시 스트레스 상황을 정의하고, 두 종류의 스트레스 유발 시나리오를 구성하였다. 실험은 신체 건강한 20-50대 남녀 30명을 대상으로, 10분간 의 운전 상황을 연출하였다. 스트레스 저감에 효과적인 자극조합을 확인하기 위해 시각, 청각, 후각의 자극을 이용하였 으며, 시각 자극의 경우 LED 조명을 이용하여 블루, 그린, 옐로우 색상을 제시하였으며, 청각 자극은 화이트, 핑크, 브라운노이즈 사운드를, 후각 자극의 경우는 로즈마리, 라벤더, 레몬향을 각각 사용하였다. 또한, 스트레스 저감에 대한 효과를 살펴보기 위해, 다감각 자극 제시 전후로 주관평가 및 심박변이도를 측정하였다. 심장 반응의 결과, 그린(조 명), 핑크노이즈(사운드), 로즈마리(향)의 자극 조합에서 HF(%) 증가 및 LF(%), LF/HF(%)의 감소를 확인할 수 있었으 며, 이는 부교감반응의 활성화가 이루어짐을 알 수 있었다. 주관평가 또한 다감각 자극에 노출되었을 때, 다감각 자극이 없는 경우보다 스트레스 저감 효과가 있는 것으로 확인되었다. 이러한 결과는 다감각 자극에 노출되기 전보다 다감각 자극을 경험할 때 운전시 스트레스 저감에 효과를 보이는 것으로 사료된다. 향후 연령별이나 성별에 대한 차이나 개인이 선호하는 자극에 대한 연구를 통해 맞춤형 스트레스 저감 자극에 대한 효과를 확인하는 것이 필요하다.

우리나라 국립공원 내 조림지 비율이 높은 6개 국립공원을 대상으로 일본잎갈나무림의 식생구조를 분석하고자 일본 잎갈나무가 우점하는 지역을 대상으로 우점비율, 흉고직경, 조림지역 등을 고려하여 조사구를 설정하였다. 이에 본 연구는 조림지 비율이 높은 국립공원을 대상으로 조림지중 가장 높은 비율을 차지하고 있는 일본잎갈나무림의 식생구조 를 분석하고, 식생유형구분을 통해 선정된 6개 국립공원의 일본잎갈나무림의 생물다양성 증진을 위한 생태적관리의 기초자료 제공을 목적으로 수행하였다. 조사구는 방형구 400㎡를 기준으로 총 51개소를 설정하였다. 군락분류는 TWINSPAN을 활용하였으며 분류된 군락별로 상대우점치, 종다양도, 종수 및 개체수 등을 분석하였다. 군락분류 결과 총 7개 군락으로 구분되었으며 7개 군락 모두 교목층에서 일본잎갈나무가 우점하는 군락으로서 아교목층과 관목층의 출현수종에 의해 구분되었다. 군락 Ⅰ, Ⅱ, Ⅶ은 계곡부에 위치하며 교목층에 일본잎갈나무가 우점하면서 느티나무, 산뽕나무, 물푸레나무 등이 출현하였고 아교목층에서 물푸레나무, 산뽕나무 등의 습지성 낙엽활엽수종들의 출현빈도가 높았다. 군락 Ⅲ~Ⅵ은 사면부에 위치하며 교목층을 중심으로 일본잎갈나무가 우점하면서 신갈나무와 상수리나무 등의 참나무류가 주요 출현수종이었으며 아교목층과 관목층에서는 교목성상의 참나무류와 습지성 낙엽활엽수종들의 출현빈도가 높았다. 6개 국립공원 일본잎갈나무림의 평균 종다양도는 1.2090으로 군락 Ⅲ이 1.5413으로 가장 높았고 군락 Ⅵ이 0.7042로 가장 낮았다. 평균 종수와 개체수는 9.35±2.90종, 226.05±89.98개체였다.

Institutional foodservice is a type of meal provided to workers in industry and affects the health and psychological wellbeing of workers and productivity. Thus, research on customer satisfaction with institutional foodservice is important. In addition to food industry and food culture developments, the requirements of institutional foodservice customers are diversifying due to COVID-19. Therefore, the purpose of this study was to identify factors that affect customer satisfaction with institutional foodservice using a user-based approach. In this study, the quality of institutional foodservice was defined using customer satisfaction or dissatisfaction, and by using in-depth interview and open coding (a qualitative research method), we derived qualities of institutional foodservice from the user’s perspective and compared these with those of previous studies. This study is meaningful as the quality of institutional foodservice was analyzed using a user-based approach, in-depth interview, and open coding and compares results with those of previous studies.

PURPOSES : The actual service life of repair methods applied to cement concrete pavement is analyzed based on de-icing agent usage. METHODS : Highway PMS data pertaining to de-icing agent usage are classified into three grades: low (1~5 ton/lane/year), medium (5~8 ton/lane/year), and high (greater than 8 ton/lane/year). The repair methods considered include diamond grinding, patching, joint repair, partial depth repair, and asphalt overlay on five major highways. The service life of each repair method is analyzed based on the usage level of the de-icing agent. RESULTS : The service lives of the applied repair methods are much shorter than expected. It is confirmed that the service life afforded by diamond grinding, patching, and joint repair methods are not significantly affected by the use of de-icing agents, whereas that afforded by asphalt overlay and partial depth repair methods is affected significantly. The service life afforded by the asphalt overlay and partial depth repair methods decreases at high usage levels of the de-icing agent (greater than 8 ton/lane/year). CONCLUSIONS : Among the repair methods considered, the service life afforded by partial depth repair and asphalt overlay is affected significantly by the amount of de-icing agent used. Additionally, the differences between the expected and actual analyzed service lives should be considered in the next-generation maintenance strategy for cement concrete pavements.