Background : Hair care products are mainly prepared by mixing chemicals and natural extracts, such as those obtained from medicinal plants. The purpose of this study was to investigate the effects of 70% ethanolic extracts from the whole plant of Houttuynia cordata and tymosin β4 powder mixtures on the growth of HaCat cells, hair follicle dermal papilla cells (HFDPC), and 3T3-L1 cells. Methods and Results : In the ratio of tymosin β4 more than 10% concentrations, the cell viability of HaCat and HFDPC cell were increased by higher tymosin β4 concentrations. The mixtures of 70% ethanolic extract of H. cordata and tymosin β4 had no toxicity potential to 3T3-L1 cell viability. In this test, the content of thymosin β4 was higher concentration, as the anti-inflammatory effect was increased. The lipid differentiation of 3T3-L1 cells adipogenesis rate significantly increased in a treatment concentration-dependent manner. Conclusion : These results suggest that a optimal mixture ratio of hair growth effect was 70% ethanolic extract of H. cordata solution 50% and tymosin β4 solution 50%. This mixture solution could be used in the development of hair care products.

Background : Lentinula edodes contain a variety of biosurgical substances and a large amount of beta-glucan, which is known for immune-boosting effects. L. edodes was cultivated in nation wide of Korea. And, almost farmhouse using imported strains from Japan and China. Therefore, this study was conducted to prove the excellence of domestic strains to replace imported strains. Methods and Results : In this study, beta-glucan content, which is a unique immune component of domestic and imported strains, was compared to the native immune component of domestic L. edodes strains and imported L. edodes strains. In this test, the Korean strains such as Baekwahyang, Sanjo303ho, Suhyanggo, Cheonbaekgo, and the imported L. edodes strains is Mori290, Moriyujiro. The analysis results are as followings. The proximate compositions were ranged 3.74 - 5.50% in ash, 15.22 - 25.84% in crude protein, 0.93 - 1.43% in crude fat, 6.95 - 9.89% in crude fiber of Korean L. edodes strains and imported L. edodes strains, respectively. Major minerals of Korean L. edodes strains and imported L. edodes strains were potassium (684 - 904.05 ㎎%), calcium (0.46 - 0.65 ㎎%), sodium (8.77 - 11.01 ㎎%), magnesium (30.45 - 33.93 ㎎%). The content of beta-glucan from Korean L. edodes strains and imported L. edodes strains were ranged from 35.69% to 38.68%. Conclusion : As the results of chemical analysis were showed the no difference between Korean L. edodes strains and imported L. edodes strains. These results are meaning that the Korean L. edodes strains quality of the components is not inferior to imported strains. We expected that the Korean L. edodes strains could replace imported L. edodes strains.

Background : Lentinus edodes are highly utilized as food and medicine, and are known to be suitable for preventing diabetes, high blood pressure and arteriosclerosis. L. edodes were known for prevent obesity metabolisms. Also, present consumers persue the healthy and thin condition. Our research perpose is determined the prevent obesity using L. edodes extracts. Methods and Results : The effect on adipocyte differentiation and the accumulation of fat in the L. edodes extract on 3T3-L1 cell preadipocyte and cell toxicity were tested. We measurement to evaluate the inhibition effects of L. edodes extracts on the growth of 3T3-L1 cell adipocytes. The tested cells were treated with L. edodes extracts by ethanolic extracts (concetrations at 10, 50, 100 ㎍/㎖) and hot water extracts (temperature at 50℃, 60℃, 70℃, 80℃, 90℃), respectively. Cell toxicity did not affect the growth of cells growth in hot water extracts. Intracellular lipid accumulation was measured by Oil red O method. The inhibitory effect on 3T3-L1 cell by treatment different ethanolic extract concentrations and temperature of hot-waters. The optimal inhibitory conditions of lipid accumulation by ethanoic extracts and hot water extracts were determined the 32.5% ethanol extracts and 33.3% 50℃ hot water extracts. Conclusion : The results of this study showed that L. edodes extracts had an potentials for reduce lipid accumulation activities. Further more we will examine about the mechanisms of anti obesity activities from L. edodes extracts. This results provides that the basis for antiobesity foods or medicines using Lentinus edodes extracts.

Background : Domestic use of cosmetics has largely been dependent on imports for the opening of cosmetics raw materials, but research has been progressing actively on natural ingredients to develop new materials. Methods and Results : The extracts of hot–water and ethanol extracts from Stachys sieboldii were tested cytotoxicity, inhibition of melanin biosynthesis and NO production activity. The cytotoxicity of the hot water extract of S. sieboldii were 98.4, 95.0, 93.9 and 90.6%, respectively, when treated with 10, 50, 100, 500 ㎍/㎖. Cytotoxicity of S. sieboldii ethanol extract was 97.1, 95.3, 94.6 and 94.4%, respectively, at the concentrations of 10, 50, 100 and 500 ㎍/㎖. Melanin biosynthesis inhibitory effect of α-MSH at 100 μM increased to 118.1% in melanin, but decreased to 92.3% in S. sieboldii ethanol extract. NO production inhibition increased to 109.2% when treated with LPS and was 103.3% at 500 ㎍/㎖ of hot water extract and 105.9% at 500 ㎍/㎖ of ethanol extract of S. sieboldii. Conclusion : The ethanol extract of S. sieboldii did not showed the cytotoxicity, and reduce NO production. Therefore, ethanol extract of S. sieboldii had an potentials for developing whitening cosmetics.

Background : Lentinula edodes (Shiitake mushroom) is a common edible mushroom with a number of potential therapeutic and nutritional applications. However, the growth of Lentinula edodes were classified in accordance with nutrients have no differences in seemingly. The growth characteristics of L. edodes were difficult to find out influenced about between oak and medium. Quantitative real-time polymerase chain reaction (qRT-PCR) is a powerful tool to analyze the mechanisms underlying the biosynthetic pathways of these substances. Methods and Results : A gene encoding amylase AMY was successfully isolated from the L. edodes using RT-PCR. The putative amino acid sequence encoded by AMY showed the highest the homology with the sequence of glycoside hydrolase family 13. We compared the amylase activity and levels of gene expression in L. edodes grown on different breeding materials (oak, and medium), strains from oak (Chunbaegko, and Mori 290), and strains from medium (Tanong, and Carrefour), respectively. Quantitative reverse transcription PCR utilizing pairs of primers specific for AMY gene expression shows that the expression of AMY was induced polysaccharide, and increased during the process of fruiting body formation in L. edodes by medium compositions. Conclusion : This result indicates that amylase may play an important role of growth in morphogenesis of medium condition growth mushroom. The present work will contribute to RT-PCR studies in L. edodes.

Background : Angelica acutiloba is a perennial herb from the family Umbelliferous. The root was used as a substitute for the crude drug. As a by-product of making cheese from whey, it has many proteins and nutritional components, but it has a drawback in that it must be stored in a refrigerator because the storage facility is short. In order to solve such problems, an attempt is made to solve the environmental and economic problems by producing a liquid fertilizer fermented with a large amount of whey. Methods and Results : In this study, we studied the fermentation of wheat germ with lactic acid bacteria (Lactobacillus brevis) and yeast (Saccharomyces cerevisiae). The preliminary experiment was conducted to determine the test concentration of 300 fold dilution and 500 fold dilution. As a result, the application of 300 times dilution liquid prepared by fermentation of whey using lactic acid bacteria showed higher growth and higher growth rate with higher number of leaves and branches. Growth response of the 500 fold dilution was higher than that of the control, but the growth of 300 fold dilution showed the highest. Conclusion : Therefore, it is considered that the 300 fold dilution of Lactobacillus can be used as a substitute for compost when cultivating medicinal plants.

This study was performed to analysis of chemical constituent in Polygonum multiflorum root (PMR) by different dry methods (hot-air dry, shade dry, and freeze dry). The results are summarized as followings; major free sugar were detected fructose, glucose, and sucrose in dried PMR based on various dry methods. The highest content of free sugars was found in freeze dried PMR. The four organic acids were detected in dried PMR by HPLC analysis. The content of oxalic acid in shade dried PMR was higher than the dried PMR by different dry methods. The content of total amino acid and essential amino acids were high in the orders of freeze drying > shade drying > hot-air drying. The potassium and magnesium levels of freeze dried PMR was significantly higher than the other drying method of PMR. Whereas the calcium and sodium levels were higher in hot-air dried PMR. The major fatty acids were determined the linoleic acid in PMR by different dry methods.

Background: Hair loss related syndromes are increasing due to environmental pollution and stress. Hair care products are mainly prepared by mixing chemicals and natural extracts, such as those obtained from medicinal plants. The purpose of this study was to investigate the effects of 70% ethanol extracts from the flowers of Calendula officinalis, fruit body of Phellinus linteus, and the whole plant of Houttuynia cordata on the growth of CCD-986 cells, hair follicle dermal papilla cells (HFDPC), and 3T3-L1 cells. Methods and Results: All sample extracts at all concentrations, except for that from P. linteus fruit body at 500㎍/㎖, were cytotoxic to CCD-986 cells. However, none of the sample extracts were cytotoxic to HFDPC. The lipid differentiation of 3T3-L1 cells regulates hair regeneration via secretion of platelet derived growth factor. The 70% ethanol extract of H. cordata whole plant promoted hair growth. Adipogenesis rate significantly increased in a treatment concentration-dependent manner. Conclusions: These results suggest that 70% ethanol extracts of C. officinalis flower, P. linteus fruit body and H. cordata could be used for the development of hair care products.

In this study, we investigated the variation in free sugars, organic acids, antioxidant activity and anti-inflammatory effect of Solanum nigrum Linne fruits according to harvest time. Four kinds of free sugars (fructose, glucose, sucrose, maltose) were detected in S. nigrum fruit, and the free sugar contents varied significantly with harvest time. Organic acid content of S. nigrum fruit showed the highest in malic acid and acetic acid, and the highest content of total organic acids was found in S. nigrum fruit harvested on October 18th and October 25th. For the total polyphenol content, S. nigrum fruit harvested on October 18th was the highest. The strongest DPPH and ABTS radical scavenging activity was showed in S. nigrum fruit harvested on October 11th and October 18th. The anti-inflammatory activity and antioxidant effects were the highest in the ethanol extract from S. nigrum fruit collected on October 18th and October 11th. Thus, it seems the best to harvest of S. nigrum fruit harvested on October 11th and October 18th.

Background : Recently, hair loss, which has been regarded as a mere means of middle-aged men due to stress and environmental pollution. The market for hair loss in Korea is about four trillion won and it is growing continuously. It is mainly made by mixing natural extracts such as medicinal plant. The purpose of this study was to investigate the effects of ethanol extracts of Houttuynia cordata Thunb. whole plant and Calendula officinalis L. flower extracts on the growth of fibroblasts, dermal papilla cells and lipid precursors, I want to try to make a materialization. Methods and Results : The cytotoxicity of each sample extracts treated with 50%, 100%, and 500 μg to fibroblasts, cell-viability were 107.3%, 109.6%, and 128.2%, respectively. The cytotoxicity of each sample to the dermal papilla cells was not observed. And the lipid differentiation of the lipogenic precursor cells which regulates the hairegeneration by secretion of the platelet derived growth factor. The 70% ethanol extracts of H. cordata whole plant and C. officinalis flower were showed promotes the hair growth activity. The lipolysis rate was significantly increased with increasing treatment concentration Conclusion : As a result of this study, in-vitro hair growth activity of herbal medicines for hair treatment material development was not shown to be toxic to each cell. And 70% ethanol extract of H. cordata whole plant stimulated lipid precursor cells inducing differentiation. As a result, the 70% ethanol extracts of H. cordata whole plant and C. officinalis flower have potential to developing hair-related product.

Background : Polygonum multiflorum Thunb. is a herbaceous perennial belonging to the Polygonaceae family. And is an herbal medicine which can be used as a raw material for food, which is excellent in immunity enhancement, vocalization and blood transfusion. The purpose of this study was to expand the utility of the P. multiflorum. Also, we fermented P. multiflorum by mushroom mycelial, and analyzed for general components and amino acids before and after fermentation Methods and Results : The moisture content of P. multiflorum and fermented P. multiflorum by mushroom mycelial (FPM) were 7.35% and 59%, respectively. The crude protein content did not show a significant difference between the two samples, crude fat, ash and crude fiber content of FPM were lower than P. multiflorum. The content of soluble nitrogen free extract of P. multiflorum (79.78%) was significantly higher than FPM (31.05%). Sixteen kinds of amino acids were detected in P. multiflorum, and the major amino acid was determined the arginine. The content of arginine and glutamic acid were 586.67 ㎎%, and 283.78 ㎎%, respectively. Sixteen kinds of amino acids were detected in FPM, and the major amino acids were determined the arginine (654.68 ㎎%) and threonine (591.18 ㎎%). The total amino acid contents of P. multiflorum and FPM were 3,469.03 ㎎%, and 3,630 ㎎%, respectively. Conclusion : The content of crude fat, ash, crude fiber, and soluble nitrogen free extract of FPM were lower than the P. multiflorum, and the major amino acids were different in two samples. Total amino acid content of FPM was higher than the P. multiflorum. As the mushroom fermentation progresses, it is confirmed that the amino acid content is increased, and it is expected to develop the product using the P. multiflorum fermented with mushroom mycelial.

In this study, we investigated the variation in free sugars, amino acids, antioxidant activity, and anti-inflammatory activity of Solanum nigrum Linne based on harvest time. Major amino acids identified by HPLC analysis were proline, histidine, and serine. The highest content of total amino acids were found in S. nigrum aerial parts and roots harvested on July 10th and August 10th. Four kinds of free sugars (fructose, glucose, sucrose, maltose) were detected in S. nigrum, and the free sugar content varied significantly with harvest time. The fructose content of S. nigrum decreased with as harvest time increased. The total polyphenol content of S. nigrum was highest in those harvested on August 30th. The antioxidant activity of ethanol extract from S. nigrum collected at different harvest times were measured by DPPH and ABTS radical scavenging assays. The anti-inflammatory activity of these extracts were assayed via nitric oxide suppression in C6 glioma cells with a lipopolysaccharide (LPS)-induced inflammatory response. The anti-inflammatory activity and antioxidant effects were the highest in the extract from S. nigrum collected on August 30th. Good correlations were observed between antioxidant and anti-inflammatory activities in ethanol extract of S. nigrum roots harvested on August 30th.

토마토 억제 재배 시 생산비를 절감하고 토마토의 수확시기를 앞당기며 잦은 경운에 의한 토양환경을 보호하고자 가지대목(EG203)을 활용한 딸기후작 토마토 무경운 재배법 연구를 실시하였다. 가지대목(EG203)을 사용하여 토마토 재배를 실시한 결과 풋마름병 발생은 무경운 재배에서 실생은 30%, 가지대목(EG203)은 0%였으며 경운 재배에서는 실생은 25%, 가지대목(EG203)은 0%였다. 가지대목(EG203)을 이용한 경운 및 무경운 재배에서 상품수량은 각각 2,693, 2,657 kg/10a로 유사하였으며 당도 및 경도도 차이를 보이지는 않았다. 따라서 딸기 후작 토마토 재배에서 풋마름병에 대한 안전성을 높이기 위해 가지대목(EG203)을 활용하여 접목을 실시하여야하며 가지대목(EG203)을 이용하여 재배할 경우는 경운과 무경운 재배의 차이가 나타나지 않으므로 경제성 및 환경적으로 무경운 재배가 유리할 것으로 판단된다. 토마토 정식 당일, 10, 20, 30일 후 전작물인 딸기를 제거한 결과 20, 30일 후 제거에서 초장과 절간장은 길었으나 다른 생육에서는 차이를 보이지 않았다. 상품 수량은 정식 당일 제거한 것이 1,885 kg/10a로 30일 후 제거 1,678 kg/10a보다 12% 많았으나 전작물인 딸기의 제거 시기는 딸기의 생육과 가격을 토마토의 기대소득과 비교 경제성을 분석하여 결정하여야 할 것으로 생각된다.

매화의 품종과 건조방법에 따른 향기성분을 분석하여 매화차 제조에 맞는 재료와 처리방법을 찾고, 매화의 첨가량 및 침출 온도, 녹차 첨가 비율 등을 관능검사를 통하여 살펴본 결과, 매화의 휘발성 향기성분의 분석결과 총 17종이 검출되었으며 그 중 생화의 경우 총 15종이 검출되었다. 동결건조 시료에서는 건조과정중 1가지 성분이 소실되었고 1가지 성분이 다르게 검출되었으며, 음건의 경우4가지 성분이 소실되었고 1가지 성분이 다르게 검출되었다. 3가지

새로운 식품개발 및 천연 보존료 개발의 일환으로 물, 에탄올, 에칠아세테이트로 추출한 항균활성 물질을 몇 종의 병원균과 식중독균 식품과 관련이 있는 세균, 젖산균 및 효모 등 10개 균주에 대하여 항균활성 검색을 하였으며, 항균력이 강한 밤잎과 수피 에탄올 추출물을 용매 계통분획하여 각 분획별 항균활성을 조사하였다. 또한 에탄올 추출물의 최소저해농도, 추출물에 함유된 항균성물질의 열안정성 pH 등을 조사하였다. 밤 잎과 수피의 항균활성은 에탄올

본 연구에서는 마늘을 여러 가지 방법으로 열처리해 새로운 형태의 식품으로 개발하기 위한 기초자료로 사용하기 위해 생마늘과 열처리한 마늘의 성분함량을 분석하였고 그 결과는 다음과 같다. 각각의 마늘의 일반성분의 분석결과 수분, 조회분, 조섬유 및 조지방 함량은 비슷하게 나타났으나 조단백질의 함량은 열처리 함에 따라 감소하였다. 생마늘의 총 구성아미노산 함량은 였고 생마늘에서 arginine이 로 가장 높았고 열처리함에 있어서는 총 구성아미노산 차이

밤의 일반성분, 유리당, 유리아미노산, 구성아미노산, 무기질, catechin 및 total polypenol을 분석한 결과는 다음과 같다. 밤의 수분 함량은 밤의 외피, 내피, 깐밤, 밤, 밤나무 수피 및 밤나무 잎 중 밤나무 잎이 로 가장 높았고, 회분 함량은 밤의 각각의 부위가 비슷하게 나타났으나 내피가 로 가장 많은 함량을 보였다. 조단백질 함량은 각각의 부위중 간밤에서 로 가장 많은 함량을 보였고 조지방 함량은 외피에서 로 가장 많은 함

조리방법을 달리한 마늘을 대상으로 새로운 식품개발 및 천연 보존료 개발의 일환으로 물, 에탄올로 추출한 항균활성 물질을 몇 종의 병원균과 식중독균 식품과 관련이 있는세균, 젖산균 및 효모등 10개 균주에 대한 항균활성 검색을하였으며, 항균력이 강한 마늘 에탄을 추출물을 용매 계통분획하여 각 분획별 항균활성을 조사하였다. 또한 에탄을안정성, pH 등을 조사하였다. 생마늘과 마늘장아찌 추출물의 항균활성은 에탄을 추출물과 물 추출물이 10균주 중 세균

This study was carried out to investigate the effect of soil-born microorganism, vitabio on growth of leaf lettuce in the vinyl house. Total weights of leaf lettuce treated with vitabio showed difference compared with untreated leaf lettuce. Sugar content was also increased. Mineral contents of leaf lettuce showed no difference between treated with vitabio and untreated with vitabio. Exchange Capacity (EC) and Organic Matter (OM) in chemical properties of soil treated with vitabio showed higher than soil in untreated vitabio. Vitabio treated soil contained much more microorganisms such as Bacteria, Actinomycetes, Hyphomycetes, Bacillus sp. Pseudomonas sp. after harvest.