Porcine beta casein promoter를 이용하여 형질전환 동물에서 유선특이적으로 목적 단 백질을 발현시킬 수 있는 pPBC 벡터를 구축하였으며 이 벡터를 이용하여 보다 높은 농 도의 목적 단백질을 발현 시킬 수 있도록 pPBC 벡터 개량을 시도하였다. pPBC 벡터의 5'arm 부위를 5428 bp, 4419 bp, 3378 bp의 길이로 잘라 서로 다른 크기의 5’arm을 갖 게 하였으며 5’arm의 5’ 쪽으로 CMV enhancer를 삽입하였다. 또한, porcine beta casein promoter 조절 하에 hGH(human growth hormone) 유전자를 발현하도록 하는 벡터를 구 축한 후 세포주 및 형질전환 마우스에서 발현 양상을 확인하였다. 개량된 pPBC 벡터를 이용하여 mouse mammary gland epithelial cell line HC11에서 luciferase assay를 통해 각각의 벡터에 대한 활성을 확인한 결과, 5’arm 길이가 가장 짧고 CMV enhancer가 삽입 된 CMV-pPBC p-3378 벡터에서 활성이 가장 높게 나타났다. 이 벡터를 이용하여 hGH 유전자와 mRNA를 안정화시켜 유전자의 발현을 증가시킨다고 알려진 woodchuch hepatitis virus post-transcriptional regulator element (WPRE)를 함께 삽입하여 CMV-pPBC p-3378-hGH-WPRE 벡터를 구축하였다. 이 벡터로 transgenic mice를 생산하여 유선을 포함한 여러 조직으로부터 hGH의 발현을 RT-PCR 방법을 확인하였다. 그 결과 liver와 lung에서는 아주 약하게 hGH가 발현을 하였으나 유선 조직에서 hGH가 가장 많이 발현 하는 것을 볼 수 있었다. 형질전환 마우스 유즙 내의 hGH 발현을 western과 ELISA를 이 용하여 확인한 결과, 유즙 내에는 약 22 kDa의 hGH가 존재 하였으며 ml 당 최고 50 100 ug의 농도로 hGH를 포함하고 있었다. 이러한 결과들은 개량한 pPBC 벡터가 유선특 이적인 발현을 하며, 유선 세포주에서는 높은 활성을 나타냈지만 형질전환 마우스의 유 즙에서는 개량전의 벡터 보다 낮은 수준의 목적 단백질을 생산하여 서로 상이한 결과를 나타내었다. 따라서 앞으로도 유선특이적 발현 벡터인 pPBC 벡터의 개량 연구를 계속 진행할 것이다.

The Korean Ministry of Education, Science, and Technology has made strenuous efforts to improve the quality of English education in public sector. One such attempt is a long-term, intensive teacher training that began in 2007 to facilitate teachers' ability for teaching English in English (TEE). This initiative has expanded over the years such that the number of training institutions has increased to 22 in 2009 to serve growing numberof trainees. This development is not without problems as there is a lack ofconsistency in curriculum, course content, quality of instructors, and overseas training among these institutions. This study aims to check the current status of various teacher training programs and suggest ideas for enhancing the quality of the programs. For the purpose of the study, questionnaires were administered to 900 English teachers to have themevaluate the training programs they had participated in. The findings from 449 respondents showed that most teachers were satisfied with their training programs. These teachers felt that the training programs helped to promote both English skills and teaching skills. Yet, they expressed some concerns about unbalanced curriculum, lack of follow-up service, inefficiency ofoverseas training, etc.

본 연구는 장소마케팅을 통한 농촌지연산업 활성화 전략을 살펴보기 위함이다. 이를 위해 순창을 사례지역으로 하여, 순창의 장소자산 특성을 통해 순창전통고추장민속마을의 위상과 핵심장소자산을 도출하였다. 이를 토대로 장류산업형․ 장류문화형․전통가치 교류형 장소마케팅 전략을 수립하였다.

본 연구는 농업의 서비스화 현상에 대한 대응 전략으로서 농촌경제 활동을 다각화하는 농촌시장 개발의 방향을 모색하였다. 먼저 농촌시장의 대두 배경을 포함한 농촌시장 형성 조건으로서 향토자원의 중요성에 대해 이론적으로 고찰하였다. 전라북도남원을 사례지역으로 현지 방문조사를 통해 농촌시장개발여건 및 향토자원을 분석하였고, 전문가 집단의 평가를 중심으로농촌시장의 개발 방향을 제시하였다. 농촌시장개발은 지역에서 생산되는 농산물 생산․판매활동을 촉진하는데 본질적인 목적을두고 마케팅 촉진 수단으로 농업․농촌의 공간적 서비스를 활용해야 할 것으로 본다.

This paper aims to study Yeats and Nietzsche through Nietzsche’s “Will to Power” in some of Yeats’s poems. In 1902, Yeats first read Nietzsche’s works; through Nietzsche Yeats’s voice turned into a manly voice. An internal conflict appears as a Mask theory in Yeats's poetry. Self and Anti-self (Mask) are two components in the Mask theory. While Will is an internal and subjective self, Mask is a social and objective self. The internal conflicts between Will and Mask determine the human mind. Yeats’s “Mask” and “Ego Dominus Tuus” exemplify Will to Power as an internal conflict. Nietzsche’s Will to Power is a concept of quantity based upon the law of energy preservation refusing causality, the movement ascending and descending and the eternal recurrence of the same. Similarly, Yeat in his A Vision has rewritten a European history based upon the theory of opposite forces in a gyre. He classifies personalities into 28 types based one phases of the moon. Assigned to Phase 12, the phase of heroic man who overcomes himself, Nietzsche is a forerunner; who is fragmentary, violent, and subjective. Perspectivism, a kind of Will to Power, is a plural and relative point of view that is classified into 4 categories; Will to Power as knowledge ("Leda and the Swan"), art ("Ego Dominus Tuus"), love ("Crazy Jane Grown Old Looks at the Dancers"), and truth ("Demon and Beast"). In conclusion, Yeats’s later poems achieve a creative and powerful voice when he thinks and speaks with Nietzsche; in particular, Nietzsche’s Will to Power, a philosophy of Being and Becoming, is echoed in some of Yeats’s later poems.

The purpose of this study was to examine the relationships of snack intake and ADHD (Attention Deficit Hyperactivity Disorder) among 284 children aged 4~7 years. The degree of ADHD (Attention Deficit Hyperactivity Disorder) were assessed by both children's mother and their teachers using Behavior check list. The subjects who had a higher ADHD (Attention Deficit Hyperactivity Disorder) score ate more pizza, hamburger, chicken, processed milk, carbonated beverage. These results indicate ADHD (Attention Deficit Hyperactivity Disorder) were related positively for snack(pizza, hamburger, chicken, processed milk, carbonated beverage etc.) intake.

With the increasing prevalence of e-learning courses in higher education, it is important to investigate how the courses are being run and examine students’ perceptions of e-learning courses to provide an optimal learning environment. An end-of-course, summative evaluation and log files in an e-learning system were used in order to describe the characteristics and students’ perceptions of three e-learning English courses in a university. The section of background information in the evaluation showed that the majority of the students taking the courses are seniors, majoring in humanities and social sciences. Factors were found to influence students’ satisfaction with the courses such as use of various multimedia, interactions between instructor and students, and among students, and instructors’ evaluation of students’ achievement. A post-hoc analysis showed evidence significant differences existed by course. These findings of the study suggest that quality assurance is an issue in setting and managing e-learning English courses, which are expected to grow rapidly in a near future due to students’ increasing need for e-learning.

This study explores solutions for the problems of the current secondary English teacher selection test in Korea. The study was performed in the form of survey including a total of 557 participants, among whom were professors teaching at the departments of English education, students majoring English education, English teachers who had taken the selection test, test-takers of the test, and public officials in charge of English education in the offices of education. The questionnaire consisted of 29 questions which were designed to elicit the participants' perception or belief about the test itself, the relationships between the test and the curricula of the department, the test-making process, and the first stage of the selectional process. Analyzing the results of the survey, the present study proposes possible solutions for the problems regarding the question types of the test, the proportions of each field of the major, the curricula of the department of English in the college of education, and the test-developing and grading process.

장미의 병해충 방제에 등록되어 있는 34종, 등록되어 있지 않은 5종, 그리고 보조제 3종, 충 42종의 농약에 대한 칠레이리응애(알, 약충, 성충)와 점박이응애(알, 성충)의 선택독성과 칠레이리 응애에 의한 점박이응애 밀도억제효과를 조사하였다. 살비제 중 acequinocyl, bifenazate, fenbutatin oxide, spirodiclofen은 칠레이리응애에 대해서 독성이 낮았으나, 점박이응애에 대해서는 독성이 높았다. 살충제는 acetamiprid, imidacloprid, spinosad, thiamethoxam, acetamiprid＋etofenprox가 칠레이리응애와 점박이응애에 대해서 독성이 없거나 적었다. 살균제 중에는 azoxystrobin, kresoximmethyl, myclobutanil, nuarimol, triadimefon, triflumizole, oxadixyl＋mancozeb이 칠레이리응애와 점박이응애에 대해서 영향이 없었다. 농약보조제인 cover와 siloxane은 칠레이리응애에 대해서 독성이 컸으나, spreader는 영향이 없었고, 점박이응애에 대해서는 독성이 없거나 낮았다. 장미시설하우스에 서 칠레이리응애에 의한 점박이응애 밀도억제효과를 조사하기 위하여, 점박이응애의 밀도가 잎당 65.3마리였을 때, 칠레이리응애를 주당 30마리 방사하였다. 방사 후 11일에 점박이응애 밀도가 잎당 3.8마리로 크게 감소하였고, 방사 후 20일에는 잎당 0마리로 밀도억제효과가 높게 나타났다. 조사기간 동안 흰가루병 방제로 살균제 4회(kresoxim-methyl, myclobutanil, nuarimol, triflumizole)와 총채벌레 방제약제를 1회(spinosad)처리하였으나, 칠레이리응애 밀도에는 영향이 없었다. 이상의 결과로 보아 장미 병해충의 종합관리체계에서 칠레이리응애에 독성이 적은 약제와 칠레이리응애를 함께 이용할 수 있을 것이다. controllability. Based upon these results, it is recommended that the developing nursing interventions to change causal attribution and self-efficacy is necessary. A number of theoretical relationships and empirical finding are confirmed by this data, and future proposals in research is suggested. 따라서 교사들의 관심유형과 실행수준에 따라 수행평가에 대한 지원을 달리함으로써 교사들의 관심도와 실행수준을 향상시켜야 할 것이며 보다 효과적인 수행평가가 실시되도록 해야 할 것이다.. 결론적으로 현재 우리나라에서는 갈색 정도의 차이 문제이지 갈색란을 좋아함을 알 수 있었고, 교육에 의해 조금씩은 고정 관념을 바꿀 수 있는 가능성을 확인할 수 있었다.ique impact at 0.70 km/s and 0.91 km/s were compared with experimental results and Eulerian hydrocode CTH simulation results. The Lagrangian code NET3D is superior to Eulerian code CTH in the computational accuracy. Agreement with the experimentally obtained final deformed cross-sections of the projectile is excellent. (3) Agreement with the experimental ballistic limit data, particularly at the high-obliquity impacts, is reasonably good. (4) The simulation result