This study was carried out to identify how a self-stretching exercise program affects pain for each body area, pain relief and job satisfaction for care workers. 20 of 40 care workers with musculoskeletal symptom were randomly selected and participated a self-stretching exercise program consisting of 15 motions. The intervention was done five times or more per weeks for 8 weeks and 1 session lasted within 15 minutes. 'Musculoskeletal symptom survey table' of the Korea Occupational Safety and Health Agency(KOSHA) and JDI(Job Descriptive Index) was used for pain on the musculoskeletal symptom and job satisfaction. Survey were done twice before and after the program. The result of this study showed that self-stretching exercise program group(SSPG) relieved from pain significantly in the shoulders(p<.01) and lumbar(p<.05), comparing to the non selfstretching exercise program group(NSPG). Although no significant difference on variations in the JDI appeared in SSPG, the significant reduction appeared from the colleague relationship and organization in NSPG(p<.05). SSPG showed the significant increase on variations in JDI from the job and organization comparing to NSPG. Especially, the improvement on satisfaction for the organization was shown(p<.05). Accordingly, the self-stretching exercise program for care workers can be said to positively affect the overall pain relief and increase on the JDI.

The purpose of this study was to determine the difference of the pinch strength according to testing posture(standing and sitting) and elbow flexion degree(0。, 45。, 90。and 135。). Forty normal young adults(male: 20, female: 20, mean age: 22.68±2.91 years) participated in this study. The methods of this study were categorized as follows: 1) One set of measurement was performed on four elbow flexion degrees(0。, 45。, 90。and 135。) in two testing postures(standing and sitting) and all subjects were measured for 3 sets testing procedures in every experimental sessions. 2) Pinch strength in various elbow flexion degree was measured after 2 min rest time, and then each test set was repeatedly performed with 5 min rest time to prevent fatigue of muscles involved in the elbow joint. The result was obtained as follow: 1) In standing posture, there was statistically significant difference at 0。and 45。, 0。and 90。, 0。and 135。, 45。and 90。, 45。and 135。, 90。and 135。. 2) In sitting posture, there was statistically significant difference at 0。and 45。, 0。and 90。, 0。and 135。, 45。and 90。, 45。and 135。, 90。and 135。. 3) Statistically, there was no significant difference between standing and sitting posture in same elbow flexion degree, however pinch strength in standing posture was higher than sitting posture.

In organic agriculture, various cover-crops have been used to control weeds. In this study, we have investigated the effect of Vicia tetrasperma (L.) Schred. (Eolchigiwandu) which is native to on major insect pests of pepper. Redpepper seedlings at 8 leaves stage were transplanted in 20th May 2009 into experimental field located in the farm of the department of agricultural biology, Suwon, Korea. A cover-crop cultivation plot was compared with a control plot mulched by black plastic-film. Density of aphids and damaged fruits by oriental tobacco budworm, Helicoverpa assulta, were surveyed ten times from 21 days after transplanting (DAT) to 82 DAT. Fifty and forty pepper plants were sampled to count the density of aphids and damaged fruits by oriental tobacco budworm per a plant, respectively. In current study, three aphid species namely, cotton aphid, Aphis gossypii, green peach aphid, Myzus persicae, potato aphid, Macrosiphum euphorbiae were collected. Overally, the density of aphids on pepper in the control plot was higher than the cover-crop plot. Especially, aphid density increased up to 67.44±26.8 in early stage of control plot, whereas aphid was not found in cover-crop plot. The rate of fruit damage by oriental tobacco budworm was significantly higher in the cover-crop plot than the control plot in early stage of pepper, however damaged fruit rate of pepper in the control plot was significantly higher since the middle of July.

We assessed the environmental risk of herbicide resistant transgenic rice (Protox) on non-target herbivore, grasshoppers (Oxya japonica japonica Thunberg). We conducted life-history experiments of grasshoppers with measuring their body weight, body length, eating amount, and feces amount between non-transgenic rice (nTR; Dongjin rice) and transgenic rice (TR; Protox rice) under laboratory conditions (Temp. 25Ð, R.H. 50-70%, Photoperiod L16:D8) in 2007. The growth of grasshoppers appeared to increase at each measuring date. We also compared the growth rate of grasshoppers between nTR and TR to examine the transgenic impact on the herbivore and we found there was no statistically signifi cant difference between the two plant types (P>0.05). We found that body weight and body length for grasshoppers were highly correlated at each of the two types of plants, nTR (0.962) and TR (0.960). The correlation of eating amount and feces amount of grasshoppers were higher nTR (0.830) than TR (0.782). The energy effi ciency of the grasshopper was not a signifi cant between nTR and TR (P> 0.05). But the molt timing of the grasshoppers for TR difference was faster than for nTR. Conclusively life-history of the grasshoppers but molt timing was not a signifi cant difference between nTR and TR. Therefore, we could conclude there was not any environment risk on herbivore from our result.

Cellular imrnortali zation is thought to be an ear ly event during tumorigenesis. Telomerase reacLi vaLion by ecLopic hTERT expression is widely used for cellular imrnortali zation. This study was a imed Lo a na lyze establi sh immortalized human ora l epithelial cells(IOEC) and to reconstruct oral precancerous lesion by Lhree dimens iona l cultures. Telomerase activity was analyzed by Telomerase assays and Telomere longLh W:lS dcLccLcd by Termina l restri ction I"ragment analysis. bTERT gene was assayecl by tbe RT-PCR. p16lNK4 a‘ pHb. CDK2. P21CIP1. p27 and p53 were examined by western blotting. Three dimensiona l cu1ture using air - liquicl inLe rl"ace was pe rl"ormed. As results. IOEC was establi shed by ectopic ex pression of catalytic subunit• of telomerase‘ h1'EH1'. which is con tinuously maintained for more tban 120 population doublings(PDs) . IOEC showecl the expression 01" h1'ER1' and h1'H mHNA‘ elongated telomere length and higher telomerase activity. These cel ls showed no ex pression of p16lNK4a with retention 01" pRb and CDK2. Expression of p21CIP1. p27 a nd p53 may have no relation to immorta li ze oraJ epithelial cell s. Three dimensional culture of IOEC showed dysplastic strat ilïed epithelia l cell s. These results may serve as a useful moclel system for the study of oral carcinogenesis.

The auroral observation has been started at Jang Bogo Station (JBS), Antarctica by using a visible All-sky camera (v-ASC) in 2018 to routinely monitor the aurora in association with the simultaneous observations of the ionosphere, thermosphere and magnetosphere at the station. In this article, the auroral observations are introduced with the analysis procedure to recognize the aurora from the v-ASC image data and to compute the auroral occurrences and the initial results on their spatial and temporal distributions are presented. The auroral occurrences are mostly confined to the northern horizon in the evening sector and extend to the zenith from the northwest to cover almost the entire sky disk over JBS at around 08 MLT (magnetic local time; 03 LT) and then retract to the northeast in the morning sector. At near the magnetic local noon, the occurrences are horizontally distributed in the northern sky disk, which shows the auroral occurrences in the cusp region. The results of the auroral occurrences indicate that JBS is located most of the time in the polar cap near the poleward boundary of the auroral oval in the nightside and approaches closer to the oval in the morning sector. At around 08 MLT (03 LT), JBS is located within the auroral oval and then moves away from it, finally being located in the cusp region at the magnetic local noon, which indicates that the location of JBS turns out to be ideal to investigate the variabilities of the poleward boundary of the auroral oval from long-term observations of the auroral occurrences. The future plan for the ground auroral observations near JBS is presented.

Recently, a heavy rainfall with high spatial variation occurred frequently in the Korean Peninsula. The meteorological event that occurred in Busan on 3 May 2016 is characterized by heavy rain in a limited area. In order to clarify the reason of large spatial variation associated with mountain height and location of low level jet, several numerical experiments were carried out using the dynamic meteorological Weather Research and Forecasting (WRF) model. In this case study, the raised topography of Mount Geumjeong increased a barrier effect and air uplifting due to topographic forcing on the windward side. As a result, wind speed reduced and precipitation increased. In contrast, on the downwind side, the wind speed was slightly faster and since the total amount of water vapor is limited, the precipitation on the downwind side reduced. Numerical experiments on shifting the location of the lower jet demonstrated that if the lower jet is close to the mountain, its core becomes higher due to the effect of friction. Additionally, the water vapor convergence around the mountain increased and eventually the precipitation also increased in the area near the mountain. Hence, the location information of the lower jet is an important factor for accurately predicting precipitation.

조류 소재에 관한 연구는 꾸준히 진행되고 있다. 본 연구에서는 미역, 다시마, 불등가사리 등 해조류를 혼합 한 발효추출물의 항산화, 미백, 보습의 활성 실험을 진행하였다. 또한 유산균으로는 김치유산균 Lactobacillus sakei균주를 이용하였다. 복합해조류 발효추출물의 생리활성 실험을 진행하였으며, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) 라디칼 소거능에서는 양성대조군 보다 높은 저해효능을 보였다. 미백효능 실험에서는 3,4-dihydroxy-L-phenylalanine (L-DOPA), mushroom tyrosinase 실험을 수행하였으며, 발효 전보다 발효 후의 미백활성이 높게 나타나는 것을 확인할 수 있었다. 한편, 해조류 소재의 유효성분인 fucose에 대한 HPLC분석을 수행하였으며, 용매조건에 대한 표준화 방법을 새로이 정립하였다. 이 연구는 복합해조류 추출물이 항산화, 미백 등 화장품 소재로서 가능성을 제안할 수 있다.

Background : Methicillin-Resistant Staphylococcus aureus (MRSA) is a multidrug-resistant (MDR) strain. Especially, MRSA is developing resistance to available antibacterial agents and causing complications in the treatment of infections related to skin, soft tissue, respiratory, bone, joint, and endovascular disorders. Therefore, antibacterial agent combination therapy appears to be a useful option, particularly in developing countries where antibiotic availability is limited. (+)-Usnic acid (UA) is uniquely found in lichens, and is especially abundant in genera such as Usnea and Cladonia. UA has antimicrobial activity against human and plant pathogens. Therefore, UA may be a good antibacterial drug candidate for clinical development. Methods and Results : In search of a natural products capable of inhibiting this multidrug-resistant bacteria, we have investigated the antimicrobial activity of UA against MRSA. In this study, the effects of a combination of UA and permeable agents against MRSA were investigated. For the measurement of cell wall permeability, UA with concentration of Ethylenediaminetetraacetic acid (EDTA) was used. In the other hand, Sodium azide (NaN3) was used as inhibitors of ATPase. These results suggest that the antibacterial effect of UA was potentiated by membrane-binding agents and ABC transporter-inhibiting agents, implying that antibacterial activity is associated with damage of the cell wall and inhibition of ATPase function by UA. Conclusion : UA and in combination with EDTA and NaN3 could lead to the development of new combination antibiotics against MRSA infection. The results of this study appear to be promising, and they are expected to enhance the use of natural products as drugs.

Background : The Production of high quality ginseng seedling become increasingly important in the cultivation of ginseng because the quality of ginseng seedling is closely related to yield of ginseng. However, it has become difficult to produce high quality ginseng seedling due to environmental instability such as an increase in the occurrence of abnormal climate and pests, and the difficulty in obtain new place to cultivate ginseng seedling. This study was carried out to analyze the growth characteristics of ginseng seedling according to application of fermented compost during ginseng seedling cultivation Methods and Results : In order to evaluate the effect of fermented compost on the growth of ginseng seedling, fermented compost containing antagonistic microorganism was prepared and applied 300kg per 10a to planned place for ginseng cultivation. After seeding of the ginseng seeds, the growth of ginseng was analysed. The pH, EC, organic matter, P2O5 and NO3-N of the compost treated group were 6.1, 2.75 ds/m, 18.97 g/㎏, 1,202 ㎎/㎏ and 287.4 ㎎/㎏, respectively, while those of non-treated group were 6.6, 0.25 ds/m, 14.67 g/㎏, 1,055 ㎎/㎏ and 8.77 ㎎/㎏, respectively. As a result of analyzing the growth characteristics of ginseng seedling, the growth of aboveground part was not significantly different between the treated and non-treated group. The root length and root diameter were 13.0 ± 1.50 ㎝ and 5.5 ± 0.27 ㎜, respectively, which were not significantly different from non-treated group. However, root weight was 1.20 ± 0.14 g, which is 1.4 times higher than that of control. Conclusion : It was confirmed that the quality of ginseng seedling was improved by compost application. These results suggested that fermented compost could be used as a material for nutrients management in ginseng seedling cultivation.

한의학에서 승마(Cimicifuge dahurica, C. dahurica), 황련(Coptis chinensis, C. chinensis), 황백 (Phellodendri amurense, P. amurense) 추출물은 해독작용을 하며 후박(Magnol obovata, M. obovata) 추출 물은 항균 효과가 있다고 알려져 있다. 본 연구에서는 승마, 황련, 황백, 후박 추출물을 혼합하여 기능성 화장품 소재로써의 이용 가능성을 검토하였다. MTT assay를 이용한 세포 독성평가와 DPPH와 ABTS를 이용한 항산 화 효과를 측정한 결과, 단일 추출물보다 추출혼합물이 더 안전함을 확인 하였으며 농도 의존적으로 항산화 활성 이 증가됨을 확인하였다. 뿐만 아니라 NO 생성을 억제함으로써 우수한 항염증 효과가 있을 것으로 예상되며, 섬유아세포에서 UVB로 유도된 세포 손상을 보호하면서 type 1 pro-collagen 합성량이 증가되는 것을 관찰 하였다. 또한 추출 혼합물은 melanin 합성과 tyrosinase 활성 및 melanin 합성 관련 인자 및 효소들(MITF, tyrosinase, TRP-1, TRP-2)의 발현을 효과적으로 억제하였다. 이러한 연구결과로 보아 승마, 황련, 황백, 후박 추출 혼합물은 항노화 및 미백 등 기능성 화장품 소재로써 개발 가능성이 높을 것으로 사료된다.

Fas ligand (FasL) and its receptor Fas have been implicated in granulosa cell apoptosis during follicular atresia. Although interferon-gamma (IFN-γ) is believed to be involved in the regulation Fas expression in differentiated granulosa or granulosa-luteal cells, the expression of this cytokine and its role in the regulation of the granulosa cell Fas/FasL system and apoptosis during follicular maturation have not been thoroughly investigated. In the present study, we have examined the presence of IFN-γ in ovarian follicles at different stage of development by immunohistochemistry and related their relative intensities with follicular expression of Fas and FasL, and with differences in granulosa cell sensitivity to Fas activation by exogenous agonistic Anti-Fas monoclonal antibody (Fas mAb). Although IFN-γ immunostaining was detectable in oocyte and granulosa cells in antral follicles, most intense immunoreactivity for the cytokine was observed in these cells of preantral follicles. Intense immunoreactivity for IFN-γ was most evident in granulosa cells of atretic early antral follicles where increased Fas and FasL expression and apoptosis were also observed. Whereas low concentrations of IFN-γ (10-100 U/mL) significantly increased Fas expression in undifferentiated granulosa cells (from preantral or very early antral follicles) in vitro, very higher concentrations (≥ 1,000 U/mL) were required to up-regulate of Fas in differentiated cells isolated from eCGprimed (antral) follicles. Addition of agonistic Fas mAb to cultures of granulosa cells at the two stages of differentiation and pretreated with IFN-γ (100 U/mL) elicited morphological and biochemical apoptotic features which were more prominent in cells not previously exposed to the gonadotropin in vivo. These findings suggested that IFN-γ is an important physiologic intra-ovarian regulator of follicular atresia and plays a pivotal role in regulation of expression of Fas receptor and subsequent apoptotic response in undifferentiated (or poorly differentiated) granulosa cells at an early (penultimate) stage of follicular development.

Biological nitrogen removal is generally accomplished by aerobic nitrification coupled with anoxic denitrification. Many commercial wastewater treatment plants (WWTPs) use external carbon source, such as methanol, to support heterotrophic denitrification process. Using organic wastes as an alternative to commercial carbon sources could thus be beneficial by saving the expense as well as reducing the environmental footprint. Here we report a full-scale (treating 2300 m3 wastewater/d) WWTP that previously utilized a butanediol-based organic waste as the sole external carbon source, which diversified the carbon sources by using a second organic waste generated from food waste recycling. Process parameters were extensively monitored for seven months at all biological unit processes, the aerobic and anoxic tanks, as well as the recirculation flow. Bacterial community structures were analyzed at anoxic tank using next-generation sequencing. The WWTP showed a stable nitrogen-removing performance over the seven months period. The estimated COD/N utilization ratio for food waste-recycling wastewater (FRW) was near 30. The bacterial populations significantly shifted during the operation. Lactobacillaceae and Prevotellaceae were the major bacterial families in the FRW, whereas the denitrification tank was populated by many families including Saprospiraceae, Nannocystaceae, Chitinophagaceae, Eubacteriaceae, and Rhodocyclaceae. Detailed discussion of the results will be presented at the conference.

Background : Ginseng, one of most famous traditional oriental medicines, has been known for a number of pharmacological properties including anti-tumor, anti-diabetic, anti-fatigue, anti-stress, anti-oxidative, and anti-aging effects. 20(S)-Protopanaxadiol (PPD), a intestinal metabolite of ginsenosides, is one of the active ingredients in ginseng. In this study, we investigated the synergistic anticancer effect of 20(S)-PPD and temozolomide (TMZ) and the mechanism of 20(S)-PPD on glioblastoma cells. Methods and Results : We examined cell viability and the morphological changes of C6 cells after treatment of 20(S)-PPD and TMZ. 20(S)-PPD showed a potent antiproliferative activity against C6 cells by triggering apoptosis. 20(S)-PPD-induced apoptosis was characterized by a dose-dependent mitochondrial damage. 20(S)-PPD and TMZ had a synergistic effect in increasing mitochondrial damage via caspase 3 activation. Conclusion : These results revealed an unexpected mechanism of 20(S)-PPD and TMZ, triggering a mitochondrial-mediated apoptosis in C6 cells. Our findings encourage further studies of 20(S)-PPD as a promising chemopreventive agent against glioblastoma.

Background : Recently, ginseng (Panax ginseng C.A. Meyer.) berry has been used as a health-promoting supplements. Also, Mulberries (Morus alba L.) fruit have been used in traditional herbal medicine to treat and prevent diabetes. In this study, we measured the cytotoxicity after fermentation of the extracts in Panax Ginseng Berry and Mulberry Fruit. Methods and Results : The extracts were prepared by decoction for 3 hours in distilled water (100 g/L). The dried extract was then dissolved in phosphate-buffered saline (PBS) in preparation for use. Cell viability was examined by an MTT assay. RAW 264.7 cells were seeded at 1 × 104/mL densities in 96-well plates. Each grouping had a non-treated group as the control. The extracts were added to each well and incubated for 24 hours at 37°C and 5% CO2. The MTT solutions (5 mg/mL) were added to each well, and the cells were cultured for another 2 hours. The supernatant was then discarded, and 100 μL of dimethyl sulfoxide was added to each well. The optical density was read at 540 nm. Conclusion : Probiotics and prebiotics modulate the composition of human and domestic animal gut microbiota. The beneficial effects may result from suppression of harmful microorganisms or stimulation of organisms which contribute in a positive way to the nutrition and health of human and domestic animal. Recently, fermentation using microorganisms for the production of more effective compounds has been extensively studied. In particular, the novel pharmacological effects of a new compound generated by fermentation have been reported. Some previous studies have demonstrated that Fermented herbal medicine extract showed better bioactivity than normal herbal Plants extract when used at the same concentration.

Background : Schisandra chinensis is being weighted difficulties in stable production, there is increasing drought damage caused by climate change as shallow rooted crops. Therefore, the study was performed for water supply capacity and growth characteristics analysis by setting the irrigation method for the drought damage reduction. Methods and Results : Test material was used sophomore V-shaped planting Schisandra chinensis. Irrigation method were surface watering, underground watering, sprinkler and untreated. Underground irrigation was irrigation that buried hose and then dug up the 15㎝. Soil moisture tension was the irrigation after fixed at -30 ㎪(23%). Irrigation timing was performed in June-July that high drought damage and made the most fruit enlargement. The main investigating items were investigated fruit growth, normal fault rate, soil moisture and EC content according to the irrigation method. Normal fruit rate according to irrigation method were appeared in sprinkler(81, 74 %)>underground irrigation(76, 69 %)>surface irrigation(76, 67%)>untreated(66, 52 %). Cluster length of yield component was determined to effective irrigation method in fruit growth the highest in sprinkler. Soil moisture contents was maintained at appropriate level with significant -30㎪(23 %) in the sprinkler. EC content low with a downward trend in underground irrigation and sprinkler. Water supply capacity according to Irrigation Method were sprinkler 40 tons, underground irrigation 85 tons, surface irrigation 138 tons. Conclusion : Appropriate watering methods for drought damage reduction of Schisandra chinensis caused by climate change was determined in the most efficient irrigation method in sprinkler that high fruit growth and normal fruit rate, lower the required of water supply capacity.

We present proton-induced single event effects (SEEs) and γ-ray-induced total ionizing dose (TID) data for 1 Gbit lowpower double data rate synchronous dynamic random access memory (LPDDR SDRAM) fabricated on a 5 μm epitaxial layer (54 nm complementary metal-oxide-semiconductor (CMOS) technology). We compare our radiation tolerance data for LPDDR SDRAM with those of general DDR SDRAM. The data confirms that our devices under test (DUTs) are potential candidates for space flight applications.

히솝(Hyssopus officinalis)은 통화식물목 꿀풀과의 여러해살이풀로 방부제, 기침, 거담제 등 일반적으 로 방향족 허브와 약용식물로 사용되어왔다. 본 연구에서는 항산화 및 미백소재의 개발을 위해 히솝을 열수와 주정으로 추출하여 각 추출물의 in vitro 상에서의 항산화 효과 및 B16F10 melanoma 세포에서 멜라닌 생성 저해효과를 평가하였다. DPPH와 ABTS assay 시험법을 이용하여 항산화 효과를 측정한 결과, 히솝 추출물의 항산화 활성이 모두 농도 의존적으로 증가하였다. 또한 히솝 추출물은 tyrosianse 활성을 저해시켰으며, B16F10 세포에서 UVB로 증가된 활성산소와 α-MSH로 유도된 멜라닌 또한 감소시키는 효과를 보였다. 멜라 닌 생합성에 관여하는 유전자의 발현에 미치는 영향을 알아보기 위해 RT-PCR을 실시한 결과 히솝 추출물에 의해 MITF, tyrosinase, TRP-2의 발현이 감소되는 것을 확인할 수 있었다. 이러한 결과에 따라 히솝 추출물은 미백개선을 위한 화장품 소재로서 이용 가능성이 높을 것으로 사료된다.

In this study, we evaluated the antidiabetic effect of a submerged culture of Ceriporia lacerata mycelium (CL01) on hematological indices, as well as protein and mRNA expression of the insulin-signaling pathway, in db/db mice. After CL01 was administrated for 4 weeks, blood glucose levels decreased consistently, and plasma insulin and c-peptide levels each decreased by roughly 55.8%, 40% of those in the negative control (p<0.05). With regard to HOMA-IR, an insulin resistance index, insulin resistance of the CL01-fed group improved over that of the negative control group by about 62% (p<0.05). In addition, we demonstrated that the protein expression levels of pIR, pAkt, pAMPK, and GLUT4 and the mRNA expression levels of Akt2, IRS1, and GLUT4 in the muscle cells of db/db mice increased in the CL01-fed group compared to the corresponding levels in the control group. These results demonstrate that CL01 affects glucose metabolism, upregulates protein and gene expression in the insulin-signaling pathway, and decreases blood glucose levels effectively by improving insulin sensitivity. More than 90% of those who suffer from type 2 diabetes are more likely to suffer from hyperinsulinemia, hypertension, obesity, and other comorbidities because of insulin resistance. Therefore, it is possible that CL01 intake could be used as a fundamental treatment for type 2 diabetes by lowering insulin resistance, and these results may prove be useful as basic evidence for further research into the mechanisms of a cure for type 2 diabetes.