Five insecticides (Acrinathrin, Dinotefuran, Emamectin benzoate, Chlorfenapyr and fluxametamide) approved for tomato cultivation were evaluated in Frankliniella occidentalis populations collected from Chungcheong province (Cheongyang, Chungju and Gongju). Leaf dip bioassay was used to evaluate resistance levels (LC50). Bioassays on Acrinathrin demonstrated higher LC50 concentration in evaluated populations. In particular, the Chungju population was 745.61 times the recommended concentration of the insecticide. Other remarkable resistance levels were recorded for the Dinotefuran with 435.06 times and 196.29 times the recommended concentrations for the populations from Chungju and Gongju, respectively. Bioassays for Emamectin benzoate, Chlorfenapyr and Fluxametamide showed low resistance to insecticides in the evaluated populations.
Yellow flower thrips (WFT), Frankliniella occidentalis is mainly controlled using chemical control methods. But the continuous use of chemical pesticides in greenhouse may contribute to development of insecticide resistance. Therefore, in this study, we evaluated the insecticidal activity of eleven insecticides against the WFT occurring in greenhouse pepper cultivation in the Gyeonggi province. The results showed no resistance in treatments with emamectin benzoate, fluxametamide, and flometoquin while high levels of resistance were recorded in treatments with acrinathrin, acetamiprid, and dinotefuran. The Anseong and Yeoju population was more resistant against spinetoram and chlorfluazuron, respectively, than populations from other regions.
Radiation dose rates for spent fuel storage casks and storage facilities of them are typically calculated using Monte Carlo calculation codes. In particular, Monte Carlo computer code has the advantage of being able to analyze radiation transport very similar to the actual situation and accurately simulate complex structures. However, to evaluate the radiation dose rate for models such as ISFSI (Independent Spent Fuel Storage Installation) with a lot of spent fuel storage casks using Monte Carlo computational techniques has a disadvantage that it takes considerable computational time. This is because the radiation dose rate from the cask located at the outermost part of the storage facility to hundreds of meters must be calculated. In addition, if a building is considered in addition to many storage casks, more analysis time is required. Therefore, it is necessary to improve the efficiency of the computational techniques in order to evaluate the radiation dose rate for the ISFSI using Monte Carlo computational codes. The radiation dose rate evaluation of storage facilities using evaluation techniques for improving calculation efficiency is performed in the following steps. (1) simplified change in detailed analysis model for single storage cask, (2) create source term for the outermost side and top surface of the storage cask, (3) full modeling for storage facilities using casks with surface sources, (4) evaluation of radiation dose rate by distance corresponding to the dose rate limit. Using this calculation method, the dose rate according to the distance was evaluated by assuming that the concrete storage cask (KORAD21C) and the horizontal storage module (NUHOMS-HSM) were stored in the storage facility. As a result of calculation, the distance to boundary of the radiation control area and restricted area of the storage facility is respectively 75 m / 530 m (KORAD21C case), and 20 m / 350 m (NUHOMS-HSM case).
Human body and head lice are obligatory human ectoparasites. Although both body and head lice belong to a single species, Pediculus humanus, only body lice are known to be a vector of several bacterial diseases. The higher vector competence of body lice is assumed to be due to their weaker immune response than that of head lice. To test this hypothesis, immune reactions were compared between body and head lice following infections by two model bacteria, Staphylococcus aureus and Escherichia coli, and a human pathogen, Bartonella quintana. Following dermal or oral challenge, the number of these bacteria increased both in hemocoel and alimentary tract of body lice but not in head lice and the viability of the B. quintana was significantly higher in body louse feces, the major route of infection to human. In addition, body lice showed the lower basal/induced transcription level of major immune genes, cytotoxic reactive oxygen species and phagocytosis activity compared with head lice. These findings suggest that a reduced immune response may be responsible, in part, for the increased proliferation and excretion of viable bacteria which are associated with the high level of human infectivity seen in body versus head lice.
The giant water bug, Lethocerus deyrollei which is the largest aquatic insect in Korea, has been designated as an endangered species by the Ministry of Environment of Korea since 1997 and is also recorded as “Vulnerable” on red Data Book of Japan (Japan Environment Agency, 2000). The populations of L. deyrollei have decreased dramatically in Korea because of loss of wetland habitats, road kill and artificial lighting. Since 2007 Holoce Ecosystem Conservation Research Institution (HECRI) has been reared L. deyrollei through breeding in captivity and 90 pairs individuals were released from 2012 to 2013. On the basis of previous field monitoring which was considered sufficient food such as fish, tadpoles and frogs and emergent plants such as Phragmites communis, Phragmites japonica, Typha angustifolia and Acorus calamus, Hyeon-cheon(E128°09’38“, N37°30’23“, 556m) was selected as a release site. After release on July 16, 2013 and carried out monitoring twice. We observed several egg clusters on July 25 and 1st larvae on July 30 respectively. Hyeon-cheon seems to be appropriate and expected to be as a major natural wet habitats for L. deyrollei.
The centipede Scolopendra subspinipes mutilans has been a medically important arthropod species by using it as a traditional medicine for the treatment of various diseases. In this study, we derived a novel lactoferricin B like peptide (LBLP) from the whole bodies of adult centipedes, S. s. mutilans, and investigated the antifungal effect of LBLP. LBLP exerted an antifungal and fungicidal activity without hemolysis. To investigate the antifungal mechanism of LBLP, a membrane study with propidium iodide was first conducted against Candida albicans. The result showed that LBLP caused fungal membrane permeabilization. The assays of the three dimensional flow cytometric contour plot and membrane potential further showed cell shrinkage and membrane depolarization by the membrane damage. Finally, we confirmed the membrane-active mechanism of LBLP by synthesizing model membranes, calcein and FITC-dextran loaded large unilamellar vesicles. These results showed that the antifungal effect of LBLP on membrane was due to the formation of pores with radii between 0.74 nm and 1.4 nm. In conclusion, this study suggests that LBLP exerts a potent antifungal activity by pore formation in the membrane, eventually leading to fungal cell death.
This study was carried out to investigate whether giant water bug, designated as an endangered species by the Ministry of Environment of Korea since 1997, can control American bull frog tadpole which has been designated as an Invasive Alien Species(IAS) by the Ministry of Environment of Korea since 1998. The experiment was conducted through food preference between fish (Crucian carp, Carassius auratus) and American bull frog tadpole(Rana catesbeiana) in laboratory condition (25±1°C) in HECRI. Potential food such as fish(weight: 8.2±0.6, length: 87.4± 1.6mm) and American bull frog tadpole(weight: 6.8±0.4g, length: 92.3±1.6mm) were inserted 5 individuals respectively in the nineteen acrylate cages with one pair of L. deyrollei simultaneously. Twice(10:00, 18:00) per a day was monitored. Once we confirmed prey was consumed by L. deyrollei, removed and replaced with same species to maintain same number immediately. Preference for American bull frog tadpole was significantly higher(93.6%) than fish(6.4%) (F=30.65, df=1, 18, P<0.0001).
Actias artemis is a members of the family Saturniidae, also known as wild silkmoths, have impressive color and size. In 2012, estimation of Actias artemis (Lepidoptera: Saturniidae) abundance in HECRI was conducted using the mark-release-recapture (MRR) method (Jolly, 1965) from mid to late May. Seven sampling events were accomplished from 19 May, 21 May, 22 May, 24 May, 26 May, 28 May and on 30 May, during the main flight of the species. Marking was made by writing numbers in the hind wing of each individual moths. Most collections were undertaken by a team of experienced four or six researches of HECRI using light trap (mercury lamp: 250W). Seven female and 58 male moths were captured in study site. The effective population size of Actias artemis was 24.9 and heterozygosity was more than 97%. Seven marked moths were recaptured, resulting in 9.7% of recapture rate. The estimated population size of A. artemis showed a peak by 133 individuals on 22 May and then declined. The estimated adult numbers of A. artemis using MRR method from minimum 168 to maximum 5,332 (p<0.05).
Damages by Tyrophagus spp. (Acari: Acaridae) on horticultural crops were newly observed in recent years especially in organic culture greenhouses in Korea. The crops damaged by the mite species were Spinacia oleracea L. (spinach), Cucumis sativus (cucumber), and Phalaenopsis schilleriana (orchid). The spinach inner leaf growth was halted affected by the feeding of Tyrophagus similis at the early stage of the shoot emergence. The young leaves of the spinach bud had small holes and the leaves were deformed as they grew up. The surface of young cucumber fruits became shiny and soft at the early damage stage and the damaged surfaces became corky as the plants grew up. The mite from cucumber was identified as Tyrophagus neiswanderi Johnston & Bruce. In phalaenopsis orchid, the flower buds became yellow and dropped and the lower side of the leaves had white spots. The damages were caused by Tyrophagus putrescentiae.
Our society is changing rapidly to a multicultural society, but if we disrespect migrant workers, married immigrants and multicultural family children, who came from foreign countries and have been incorporated as our society members, only because of their different skin color, appearance and language and treat their culture with prejudice, such racial discrimination will cause cultural conflicts and undermine the stability of Korea society. In order for them to be the true members of our society, we need to give much consideration not only to national policies but also to social consensus and school education for minimizing their social and psychological conflicts with our culture and reduce social consumption resulting from such conflicts. Culture refers to internal ways of thinking, external behaviors, etc. shared by a specific group through a long process of life experience. Traditional culture is formed and developed collectively by a life community, and reflects their production activities and sentiments, so it has unique characteristics distinguished from the culture of other groups. Traditional culture defines the order and sentiment of life in the group members, and gives a sense of belonging to the community so that the members have identity. Multi‐culture originally meant the coexistence of diverse native cultures retained by minority races and people with their originality preserved rather than being integrated into the dominant culture in multi‐racial and multi‐ethnic countries like the U.S. These days, however, it has an expanded meaning, that is, respecting the originality of minority cultures and pursuing the coexistence of such cultures in the native culture of the mainstream society. In the process that a nation develops its culture, it may receive foreign cultures and change its traditional culture. What is important in this process is developing new culture without losing traditional culture. If traditional culture declines and fades away, overwhelmed by powerful foreign culture, it cannot be desirable succession and development of culture. Multicultural education originated from education for understanding the national background and cultural contributions of American immigrants in the late 1920s, and is also related to the term ‘national studies’ for enhancing the self‐identity of minority peoples in the early 1960s. From the late 1960s, the term ‘multiethnic education’ was used to mean that all children should learn various cultures in the U.S., and in the 1970s, the term ‘multicultural education’ began to be used as its meaning today. Banks, a representative scholar in multicultural education, defined multicultural education as “A reform movement for giving peoples from diverse genders, languages, social classes, races and groups equal opportunities for learning,” and Bennett explained it as “an approach to teach and learn based on democratic values and beliefs in order to promote cultural diversity in a culturally diverse society or an independent world.” Moreover, Morrison said that multicultural education is education for preparing students to understand, perceive correctly and respect others from different races, genders, socio‐economic statuses, languages and cultural backgrounds, and ultimately to live, communicate, and act for common goals in multicultural society.In this way, multicultural education is a concept based on diversity and pluralism as well as on democracy, equality, human rights, social justice, etc. Furthermore, targeting not only children from migrant worker, married immigrant and multicultural families with minority culture but also all students from mainstream culture, it aims to help them recognize that our society is now entering into a multiethnic and multicultural society and be able to develop harmonious relations in the multiethnic and multicultural society. In other words, multicultural education is to emphasize and teach that, in the present age of globalization, groups of different culture, race and society should maintain their own native language and culture and, at the same time, coexist with the mainstream culture and make various contributions to the society. Multicultural education can be divided into ‘multicultural education for mutual understanding among cultures,’ ‘education responding culturally,’ ‘cross‐cultural education,’ ‘cultural pluralism of education,’ ‘multicultural education as human experience,’ etc. Moreover, multicultural education has been developed through processes such as multiple culture education, international understanding education, anti‐prejudice education, and globalization education, and may share conceptual denotations with human right education, anti‐war peace education, and ecological environment education. In other words, multicultural education intends to help students understand the diversity of people with various backgrounds in terms of culture, race, gender and social class, have positive attitude and perception toward the diversity, admit cultural differences from the viewpoint of cultural relativism, and acknowledge values in other cultures. It was from the 7th National Curriculum that Korea began to take interest in multicultural education. At that time, people began to recognize the importance of international understanding education and global citizen education and the necessity to understand culture diversity in other countries. In response to these demands, the 7th National Curriculum introduced some contents for international understanding and multicultural understanding mainly in subjects related to foreign languages and social studies. Later the 2007 Revised National Curriculum reflected multicultural education in all the subjects. That is, the Generals of the 2007 Revised National Curriculum suggest multicultural education as one of 35 interdisciplinary themes of learning, and furthermore, direct that it should be reflected in the development of textbooks. The Korean government is executing various multiculturalist policies to cope with transition to multicultural society and resultant problems. That is, as the number of multicultural family children attending elementary and secondary schools is increasing with the expansion of married immigrants and migrant workers and there should be strengthened educational support to them, the Ministry of Education and Human Resources Development made Plans to Provide Educational Support to Multicultural Family Children but most of the contents aim to help foreign workers and married immigrants adjust themselves to racially homogeneous Korean society by learning Korean language and traditional culture. This suggests that while our society is changing rapidly to a multicultural society but multiculturalist policies and multicultural education are still insisting on Koreanization through infusing the myth of homogeneous race and culture and forcing assimilation to Korean national culture. What is more, through its Study on Teaching‐Learning Support for Multicultural Education, Korea Institute of Curriculum and Evaluation suggested the objectives of multicultural education as follows: first, to understand cultural diversity and differences and strengthen cultural identity; second, to develop tolerance of understanding and respective native culture as well as other cultures and the attitude of contributing to the recreation of culture; and third, to promote a sense of community, the qualities of democratic citizen, peaceful settlement of conflicts, cooperative attitude, respect for human rights, and social justice. In order to achieve these goals, in addition, it proposed programs: first, to provide opportunities to experience other cultures; second, to understand the own traditional culture; and third, to promote international understanding. However, these proposals focus on education for multicultural family children, so are not sufficient for giving multicultural education to ordinary students. In multicultural society resulting from globalization, our education should seek mutual understanding and coexistence of different languages and cultures of diverse races and people rather than the transfer of traditional culture based on our homogeneous language and race. Moreover, education in multicultural society should place emphasis more on the majority’s understanding and tolerance of minorities’ race, people, and culture than on assimilation of immigrants from foreign countries to the mainstream society. Accordingly, though already late, Korean people should also admit ‘multiculturalism’ as a basic attribute of the society instead of clinging to the purity of language and people, and receive and respect cultural diversity for the coexistence of multiple cultures in one society or one country. That is, school education should include not only education for multicultural family students to understand our language and culture as the mainstream but also education for the majority of our society to understand and tolerate the cultures of minorities including immigrants from foreign countries, half‐blooded children from them, and North Korean defectors. The fundamental objective of Korean education is “to build up the people’s character under the ideology of Hongikingan (devotion to the benefits of mankind) and to equip individuals with abilities for independent life and qualities as a democratic citizen so that they may be able to lead a noble life and to contribute to the development of democratic state and the realization of the co‐prosperity of mankind.”
Co‐prosperity of mankind is impossible without overcoming nationalism, which gives the top priority to the superiority and benefits of my own country. That is,the true co‐prosperity of mankind is possible only when we acknowledge and respect differences in history, culture, religion, language and customs between my people and others. This means that, in order to educate people required in the age of globalization, even the education of traditional culture should concentrate on raising global citizens from the viewpoint of multiculturalism.
Background : Ginseng berry(GB) is useful not only just in growing source but also in functional food source. The ingredients of crops varies with the maturity. So, GB ingredients need to be analyse for optimal harvesting stage of GB against appropriate use. Methods and Results : This study was carried out to determine optimal harvesting stage of GB. GB was harvested 5 day periods from July 12, started harvesting when pollination was 50 days old, until August 1. GB was analysed color, ginsenosides and fatty acids using colorimeter, LC and GC, respectively. As the majority of GB increase, color of freeeze drying GB powder were changed that lightness and yellowness was increased, redness was decreased. Ginsenoside Re, Rb1 and Rb2, major ginsenoside in GB, were increased and Ginsenoside F1, Rk1 and Rg5, minor ginsenoside, were increased for a time and then decreased. Oleic acid, the main fatty acid in GB, was decreased, and linoleic acid and total fatty acid content was increased to July 27 and then decreased. Conclusion : Total ginsenosides content was the highest on August 1 and total fatty acid content was the highest July 27. As the majority of GB increase, ratio of oleic acid on total fatty acid was decreased and linoleic acid was increased. Thus, GB is that the longer a harvest period and the more useful for food source.
Mannitol is commonly used to reduce intracranial and intraocular pressures and to prevent dialysis-disequilibrium syndrome. However, intravenous mannitol infusion in various cases has the potential to result in acute kidney injury (AKI). We present a case of mannitol-induced AKI that developed after low dose mannitol infusion and resulted in recovery after hemodialysis. A 66-year-old woman was admitted to the hospital with a diagnosis of left middle cerebral artery infarction. On hospital day 5, cerebral edema was observed on a follow-up MRI. D-mannitol 35 g was given intravenously every 8 hours. Four days later, serum creatinine levels were elevated from 1.2 mg/dL to 3.5 mg/dL. The serum osmolal gap was found to be 52.4 mosm/kg H2O and urine output was reduced from 2.78 mL/kg/h to 0.69 mL/kg/h over three days. Hemodialysis over 2 hours was performed and renal function subsequently improved to baseline function. A potential risk of AKI exists even with low dose mannitol infusion in patients with advanced age, underlying renal impairment, and concomitant use of nephrotoxic agents. Mannitol-induced AKI may be rapidly reversed by short-term hemodialysis.
Mungbean (Vigna radiata) is a fast-growing, warm-season legume crop that is primarily cultivated in developing countries of Asia. We constructed a draft genome sequence of mungbean to facilitate genome research into the subgenus Ceratotropis and to enable a better understanding of the evolution of leguminous species. The draft genome sequence covers 80% of the estimated genome, of which 50.1% consists of repetitive sequences. In total, 22,427 high confidence protein-coding genes were predicted. Based on the de novo assembly of additional wild mungbean species, the divergence of what was eventually domesticated and the sampled wild mungbean species appears to have predated domestication. Moreover, the de novo assembly of a tetraploid Vigna species (Vigna reflexo-pilosa var. glabra) provided genomic evidence of a recent allopolyploid event. To further study speciation, we compared de novo RNA-seq assemblies of 22 accessions of 18 Vigna species and protein sets of Glycine max and Cajanus cajan. The species tree was constructed by a Bayesian Markov chain Monte Carlo method using highly confident orthologs shared by all 24 accessions. The present assembly of V. radiata var. radiata will facilitate genome research and accelerate molecular breeding of the subgenus Ceratotropis.
Rice blast (Magnaporthe oryza B.) is one of the most widespread and devastating diseases of rice. Screening of valuable genetic resources harboring resistance genes is one of the most efficient approaches against blast disease. Because the bioassay to rice blast in the field shows high variations, this study has performed to provide DNA profiles in the accessions of diverse countries using major blast resistant genes linked markers, identified and mapped in different genotypes of rice. Because durable resistance to blast is controlled by a combination of major resistance genes, we surveyed the distribution of blast resistant genes in the 1,500 accessions using major 12 blast resistance genes linked markers. These resistant genes found that the frequency distribution of Pi-39 (66.9%), Pik-m (41.9%), Pit (40.5%), Pii (21%), Pib (19.3%), Pi-d(t)2 (12.7%), but Pita, Pita/Pita-2, Pik, Piz-t, Pi5 genes were identified in less than 10% frequency. Most of accessions contain from 1 to 4 different resistant genes. Pi39 and Pik-m genes amplified in the 69.1% and 51.7% among 356 Korean accessions, Pi39 (79.6%) and Pib (55.8%) in 113 China, Pit (80.6%) and Pib (32%) in 103 Philippines, respectively. In this study, we evaluated the blast resistance degree and the information about the distribution of rice blast resistant genes in rice germplasm. This study will help to develop effective strategies for managing rice blast disease in rice germplasm.