To investigate the quality characteristics of domestic rosé wines marketed in Korea, 11 kinds of wines were purchased at the Korea Wine Festival in 2016. The color, physiological activity, aroma component and sensory evaluations were conducted. The hue value of rosé wines ranged from 0.592 to 1.990, with the Ro7 of Delaware having the highest value. The brightness of rosé wines ranged from 42.96 to 94.99, the redness from 3.20 to 59.37, and the yellowness from 8.43 to 24.83. Of the 11 rosé wines evaluated, 1 was a dry wine and 10 were sweet wines. The average sugar content of the sweet wines was 73.89 mg/mL. Among the organic acid contents, malic acid ranged from 0.214 to 2.903 mg/mL, and lactic acid content ranged from 0 to 3.423 mg/mL. Malolactic fermentation differed, depending on the source. The total polyphenol content of rosé wines ranged from 50.55 to 99.55 mg%, the anthocyanin content was 2.12 to 213.30 mg/L, and the DPPH radical scavenging activity of rosé wines was between 73.75 to 90.41%. A total of 41 volatile components were detected, including 7 alcohols, 22 esters, 4 terpenes, 3 acids and 5 other compounds. Of these, 9 compounds had odor activity value(OAV) greater than 1; these were identified as 1-propanol (alcohol, pungent), 3-methyl-1-butanol (harsh, nail polish), ethyl butanoate (strawberry, lactic), isopentyl acetate (fresh, banana), ethyl hexanoate (green apple, fruity), ethyl octanoate (pineapple, pear, floral), ethyl decanoate (fruity, fatty, pleasant), linalool (flower, lavender) and limonene (lemone, orange). As a result of the sensory evaluation, the Ro5 wine made from Campbell Early grape, and having a good color, flavor and taste, was the most preferred.

To investigate the aroma and quality characteristics of wine bestowed by the degree of ripening of Cheongporang grapes, the general quality composition, color, organic acid, physiological activity, aroma component and sensory evaluation were analyzed. The general characteristics of the wine assessed were pH 3.34~3.50, total acidity 0.46~0.67%, color intensity 0.074~0.116, lightness 98.99~99.74, redness －0.39~0.01, and yellowness was 1.52~3.10. Malolactic fermentation was not relative to the contents of malic acid and lactic acid. The total polyphenol content and antioxidant activity were lowest in C3 wines made with ripe grapes and highest in C4 wines made with mixed grapes. Aroma analysis identified 6 alcohols, 15 esters and 5 miscellaneous compounds. Compounds with higher odor activity value (OAV) were 3-methyl-1-butanol, ethyl butanoate, isopentyl acetate, ethyl hexanoate, ethyl octanoate, ethyl decanoate and octanoic acid. The C4 wine, a mixture of grapes with different degrees of ripening, had the highest ester content and ratio. The lowest values were found in the C3 wine made with ripe grapes. Results of the sensory evaluation related the C4 wine, containing a large amount of ester compounds having high OAV values as the aroma component, as the best and most preferred wine.

This study investigated the quality characteristics and volatile flavor components of aronia wine (Aronia melanocarpa (0~100%)). After 12 days of fermentation, the alcohol contents of aronia wines ranged between 9.0~12.0%. The pH level and total acidity of aronia wines were 3.20~3.68 and 0.57~0.76 g/100 mL, respectively. The organic acid analysis of wine containing 100% aronia, revealed malic acid content at 3.70 mg/mL, followed by tartaric acid, lactic acid, and citric acid. As the aronia content increased, both the total polyphenol content and the antioxidant activity (the DPPH radical scavenging activity) also significantly increased. The total polyphenol content was the highest in the wine with 100% aronia (461.33 mg%), and the antioxidant activity showed the highest values in the wine with 100% aronia (91.91%). Volatile flavor component analysis of aronia wines identified 8 alcohols, 12 esters, 4 ketones, and 7 other compounds. In the sensory evaluation, the color, flavor, and taste of wine with 20% aronia showed higher values than other aronia wines. Based on the results of the present study, we suggest that 20% aronia is most beneficial in improving the quality as well as sensory characteristics of the wine.

This study was carried out to evaluate the quality characteristics of Korean domestic commercial white wines. Four sample wines were analyzed in terms of their pH, total acidity, volatile acidity, alcohol content, soluble solid, free SO2, total SO2, color, free sugar content, organic acid, volatile compounds, total polyphenol content, DPPH radical scavenging activity and sensory characteristics. The pH of wines ranged from 3.06 to 3.76 and their total acidity from 0.43 to 0.83% (v/v). The alcohol content of samples ranged from 9.4 to 12.8% (v/v) and their soluble solid contents from 9.9 to 12.3 °Brix. The free SO2 and total SO2 contents of the W1 wine were lower than those of the other wines, while the color intensity, a (redness) and b (yellowness) of W1 wine were higher than the values of the other wines. The malic acid contents of the four wines indicated that complete malolactic fermentation (MLF) occurred in W1, incomplete MLF in W3, and was absent in W2 and W4. Aroma analysis resulted in identification of 10 alcohols, 11 esters, 2 ketones and 6 miscellaneous compounds. 2,3-Epoxybutane and 1-(ethenyloxy)-pentane levels in were significantly higher W1 than the other wines, while several types of ester predominated in W2, W3 and W4. W1 had the lowest sensory score among the four wines. Therefore, the quality characteristics of domestic white wines are considered to be acceptable.

The recovery of epididymal sperm in animals is considered as one of the important tools to preserve high value or endangered species. However, there are no appropriate castrating indicators such as months of age in bull, sperm morphology, and motility, particularly in young Korean native bull (Hanwoo). Therefore, this study aimed to investigate sperm number, morphology, and motility of sperm in the epididymis tail of young Hanwoo bulls at 8 and 15 months of age. After castration, epididymal tails were collected and minced with blades to recover sperm. In experiments 1 and 2, sperm number, morphology, and motility were examined. Total number of sperm and percentage of normal sperm from bulls at 8 months of age was lower than that of bulls at 15 months of age after collection (P<0.05). Percentage of abnormal head, tail, proximal cytoplasmic droplet, dead and damaged acrosome of sperm from bulls at 8 months of age were higher than those of bulls at 15 months of age (P<0.05). In experiment 3, sperm motility from bulls at 8 and 15 months of age were examined before freezing and after thawing. Frozen-thawed sperm at 8 months of age showed low total motility and motile sperm with ≥ 25 μm/sec compared to those at 15 months of age and commercially-used sperm (P<0.05). In conclusion, sperm derived from the epididymal tail of bulls at 8 months of age showed high abnormal morphology and poor motility, which are not adequate for AI and IVF. On the other hand, sperm derived from the epididymal tail of bulls at 15 months of age showed high normal morphology and motility.

Data on primal cuts were collected from 1,829 steers of Hanwoo progeny testing programs, between 2010 and 2015 for the ssGWAS. SNP data were analyzed by using Illumina Bovine 50K Beadchip. The SNP data that matches with phenotype data was 674 animals. As a first step, the genomic estimated breeding value(GEBV) of the loin and rib cuts were estimated, which was used in the estimation of SNP marker effects and their variances related to the traits. Then, the estimated variance explained by each marker was expressed as a proportion to the total genetic variance. Finally, the SNP loci and their significance to any possible QTL were examined. Among the 20 best SNP loci explaining a larger proportion of SNP variance to the total genetic variance for tender loin yield, the region between 12,812,193 ~ 12,922,313bp on BTA 10 harbored a cluster of SNPs that explained about 7.32 to 7.34% of the total genetic variance. For strip loin yield, a peak for higher effects for multiple SNPs was found in BTA24, between 38,158,543 and 38,347,278bp distances, which explained about 8.36 to 8.56% of the observed variance for this trait. For loin yield had relatively smaller effects in terms of the total genetic variance. Therefore, loin yield might be affected by a few loci with moderate effects and many other loci with smaller effects across the genome.

The aim of this study was to compare ultrasonic measured traits (Eye muscle area : EMA, backfat thickness : BF and marbling score : MS) of Hanwoo cows by months of age and periods around 1st calving. The data used in this study were taken 2,636 cows which were measured from 20 months old to 28 months old and within 5 months around 1st calving. According to comparison of cows' month of age, ultrasonic measured EMA, BF and MS were not significantly different between 21 months old and 26 months old, 21 months old and 25 months old and 23 months old and 27 months old, respectively. According to comparison of periods around 1st calving, ultrasonic measured EMA, BF and MS were not significantly different between -45 days and 30 days, -15 days and 75 days, -105 days and 90 days around 1st calving, respectively. The selection of cows could be possible based on the phenotypic ultrasonic measurement performance if the traits are measured between 23-month old and 25-month old and between - 15 days and 30 days around 1st calving.

본 연구에서는 현재 국가단위 개량체계에서 이용하는 도체형질 및 12개월령 체중과 부분육간의 유전 모수추정을 통하여 각 형질별 상관관계를 조사하였다. 분석에 이용된 형질은 1996년도부터 2013년까지 당·후대 검정한 한우 수소 및 거세우 총 10,351두의 부분육 중량 및 수율, 도체형질 그리고 12개월령 체중측정 자료이다. 유전모수 추정을 위하여 부분육 형질에는 차수, 검정지, 도축일의 고정효과와 도체 중을 공변량으로 포함하였으며, 도체형질은 동일한 고정효과에 도축일령을 공변량으로 하였다. 유전모 수 추정 결과 부분육 수율(%)의 유전력은 안심, 등심 및 채끝이 각각 0.53, 0.58 및 0.65였다. 등심수 율은 12개월령 체중, 도체중, 등심단면적, 등지방두께 그리고 근내지방도와 유전상관이 각각 0.13, 0.15, 0.61, -0.44 그리고 0.37이었다. 따라서 등심단면적이 크면 10가지 대분할육 중 경제적가치가 가장 높은 형질인 등심의 비율이 증가 할 것으로 사료되며, 도체의 육질을 결정하는데 주요한 형질인 근내지방도의 등급도 다소 높아질 것으로 사료된다.

The aim of the study was to investigate the ability of sperm derived from the epididymis in regard to sperm motility, sperm penetration to oocyte and subsequent development of the embryo. Frozen-thawed sperm from epididymis showed similar percentage of motile sperm (VSL ≥ 25 μm/sec) as compared to that of commercial sperm (control). Sperm penetration of frozen-thawed epididymal and commercial sperm was not significantly different. Moreover, cleavage and blastocyst rates were similar in both epididymal and control. Sperm derived from the epididymis also showed fertilizability and subsequent embryonic development

The objective of this study was to estimate the effects of test seasons on backfat thickness and age at 90 kg body weight in Duroc pig populations. Data of a total of 40,228 Duroc pigs performance tested from 2005 to 2014 were used. The effects of sexes, years and seasons of the tests were significant (p<0.01) for the both traits. The least squares mean of the age at 90 kg body weight of the pigs tested in 2014 was significantly less than that of the pigs tested in the previous years. And the pigs tested in spring reached 90 kg body weight faster than the pigs tested in the other seasons. The least squares mean of backfat thickness of the pigs tested in autumn was thicker than that of the pigs tested in the other seasons. Pigs tested in spring had the thinnest backfat. There were seasonal variations in the least squares mean estimates of the age at 90 ㎏ both in male pigs (134.06 to 134.36 days), and in female pigs (139.47 to 139.65 days). Seasonal variations were also detected in least sqaures means of the backfat thicknesses in male pigs (11.31-11.34 ㎜) and in female pigs (13.05-13.07 ㎜). The simple and rank correlation coefficients between breeding values estimated using the trait values unadjusted for seasonal effects and those using the trait values pre-adjusted for seasonal effects trait values were all unity, for the both traits. These results indicate that the adjustment of the trait values with regards to seasonal variation had no effects on the estimates from genetic evaluations.

The objective of this study was to estimate genetic parameters on days to 90 kg body weight and ultrasound measured traits (back fat thickness, eye muscle area, retail cut percentage) and predict genetic progress when the weights for selection on the paternal traits of Duroc, which can be incorporated into sire line selection indexes. A simulation program, ZPLAN+, was used for the simulation. Genetic parameters of days to 90 kg body weight (D90KG), back fat thickness (BFT), eye muscle area (EMA) and retail cut percentage (RCP) used in the simulation were estimated from 27,972 heads of farm tested Duroc pigs from 2005 to July, 2012. Parameters of simulation used genetic parameters with parturition rates, ratios of test pigs and replacement rates that were dependent on selection protocols of simulated farms. Different weights of selection for traits of days to 90 kg and backfat thickness were compared. Estimates of heritability were 0.406, 0.355, 0.159 and 0.379 for D90KG, BFT, EMA and RCP, respectively. Genetic correlation of D90KG with BFT, EMA and RCP were –0.043, 0.049 and 0.021, respectively. Genetic correlation of BFT with EMA and RCP were –0.394 and –0.937. And genetic correlation between EMA with RCP were 0.524. Simulation result showed that index weight of 3:1 on D90KG and BFT was effective to shorten days to 90 kg without any significant changes in backfat thickness. Generally the genetic changes were greater as parturition rate and ratios of test pigs increased. Increase in replacement rate had a small effect on genetic changes in negative way. Thus, we recommend that index weight of 3:1 on days to 90 kg and backfat thickness could be utilized for sire line selection.

Embryo transfer (ET) technology is of high importance in modern cattle breeding programs. ET is one step in the process of removing one or more embryos from the reproductive tract of an outstanding donor female and transferring them to one or more recipient females. Embryos also can be produced in the laboratory via techniques such as in vitro fertilization (IVF). But the actual transfer of an embryo is only one step in a series of processes that may include some or all of the following: superovulation and insemination of donors, collection of embryos, isolation, evaluation and short-term storage of embryos, micromanipulation and genetic testing of embryos, freezing of embryos and embryo transfer. Cryopreservation and direct transfer of frozen-thawed embryos is common-place with pregnancy rates near that of fresh embryos. Polymerase chain reaction (PCR) technology is currently being used for sexing embryos, and this technology will be used for “embryo diagnostics” and “embryo genomics” in the future. Although, many limitations and problems remain to overcome, these and other new technologies promise to change livestock breeding drastically in the next decade.

Economic traits are quantitative traits and are mostly controlled by a large number of genes. Some these genes tend to have a large effect on quantitative traits in cattle and are known as major genes primarily located at quantitative traits loci (QTL). However, in practice, QTL is linked to allele associates of the gene controlling traits of interest. It is hypothesized that if QTL explaining a part of genetic differences between animals are detected, the effect of the genes located at QTL could assist in estimating an animal’s true genetic value. Therefore, QTL information could probably provides accuracy of breeding value estimation as well as more genetic gain through selection of animals at relatively younger age. Marker assisted selection (MAS) is the indirect selection process where a quantitative trait of economic importance is selected not just based on the trait itself but also on the basis of marker linked to QTL. MAS could be useful for traits that are difficult to measure, exhibit low heritability, and are expressed late in development. Major genes which are responsible for QTL could possibly be identified first by using different techniques such as gene expression analysis and QTL mapping. Thereafter, the information generated could be implemented for MAS in estimating breeding value. In this review we focused on delivering genome information into Hanwoo breeding program.