This study evaluates the gastroprotective effect of cabbage extract with sulforaphane content of 5.19 mg/L and Smethylmethionine content of 469.28 μg/L. In vitro, the lipopolysaccharide (LPS)-treated group had an increased NO activity compared to the normal group, and the concentration of NO was reduced when the cabbage extract was treated in the dose manner. The level of IL-6 induced by LPS was dose-dependently reduced when the extract was treated. The cabbage extract concentration was orally administered in rats at 5.75 mg/kg, 11.5 mg/kg, and 23 mg/ kg, and the inhibitory effect on gastric damage by HCl-ethanol was observed. Histological analysis exhibited mucosal erosion in the gastritis model compared to the normal group, while the ameliorating effect of the generated erosion was observed in the cabbage-treated group. The histamine concentration was significantly increased in the gastritis-induced animal model, and the histamine concentration was decreased in the 23 mg/L-treated group of cabbage extract. In conclusion, the results of this study suggest that cabbage extract not only down-regulates cytokines in vitro, but is also directly involved in histamine secretion in an animal model of gastritis; therefore, cabbage extract can help inhibit gastrointestinal disorders by improving the protective barrier.

Traditionally, mistletoe is known as an effective anti-cancer medicinal plant, and lectin is recognized as a major component with cytotoxic and immuno-stimulant activity in mistletoe. A Korean mistletoe lectin (KML) has specificity to galactose and galactosamine and is distinguish from European mistletoe lectin (EML). When we examined the concentration of lectin in mistletoe originated from five different types of host trees, the result indicate that the lectin concentration is variable depending on the host tree. Noticeably, mistletoe from chestnut tree contains ten folds higher lectins than that of an oak tree. We also tested the concentration of KML and crude extract (KM-110) of Korean mistletoe that shows 90% cytotoxicity in L5178Y-ML25 lymphoma cell. In addition, the cells show 90% and 70% viability by the treatment of two neutralizing antibodies of KML, 9H7-D10 and 8B11-2C5 neutralization effect with two monoclonal antibodies of KML, 9H7-D10 and 8B11-2C5. Therefore, the result expected that the mistletoe contain some other cytotoxic components except lectin. Finally, the production of TNF-α and IL-6 by RAW 264.7 cells stimulated with lectin free-crude extract (LFKM-110) following neutralization by 9H7-D10 monoclonal antibody shows higher than that of lectin containing-crude extract (KM-110). These results suggest that the Korean mistletoe lectin ha a great potential to be developed as therapeutic agent of cancer.

본 연구에서는 겨우살이 열수 추출물인 미슬로 C의 안전성을 검토하고자 유전 독성 및 실험동물을 이용한 안전성 검사를 실시하였다. 미슬로 C의 미생물 돌연변이 실험을 S. typhimurium의 히스티딘 요구성 균주와 E. coli의 트립토판 요구성 균주를 이용하여 대사 활성계 적용 및 비적용 하에서 복귀돌연변이 시험을 실시한 바, 5,000 ㎍/plate의 처리 농도까지 복귀돌연변이 집락은 나타나지 않았다. ICR 마우스에게 500, 1,000 및 2,000 ㎎/㎏를 경구 투여하고, 골수세포를 수집하여 소핵을 측정한 결과, 정상마우스의 경우와 비교하여 유의한 소핵은 관찰되지 않았기에 미슬로 C는 유전독성을 유발하지 않는 것으로 판단되었다. 식품의약안전청의 의약품 등의 독성시험기준에 따라 암․수 SD 계열의 랫드에 시험물질을 0, 500, 1,000 및 2,000 ㎎/㎏/day의 용량으로 1회 경구 투여한 후, 14일간의 체중 변화 및 사망률을 조사한 결과, 대조군과 비교하여 유의한 체중 변화는 없었으며, LD50은 2,000 ㎎/㎏ 이상인 것으로 사료된다. 또한 0, 250, 500 및 1,000 ㎎/㎏/day의 용량으로 13주간 반복 투여하면서 실험동물의 일반증상, 체중변화, 혈액 및 혈액생화학적 변화, 부검소견, 조직학적인 변화를 관찰하였다. 시험기간 중 암․수 모든 군에서 시험물질 투여에 기인한 일반적인 증상 변화는 관찰되지 않았고, 시험물질의 반복 투여로 인한 사망 마우스 역시 관찰되지 않았다. 따라서 미슬로 C를 13주간의 랫드에 대한 13주 반복 경구투여 결과, 무독성량은 최소한 1,000 ㎎/㎏ 이하인 결과를 나타냈으며, 이 농도에서 독성을 유발하는 표적장기는 관찰되지 않았다.

The present study is designed to explore an anti-tumor activity on crude extracts of Oplopanax elatus. Water extractions of Oplopanax elatus were performed at 100℃(OeE-100). OeE-100 doses up to 62.5 ㎍/㎖ had no cytotoxicity on the tumor cell lines in vitro. In experimental lung metastasis of colon26-M3.1 carcinoma or B16-BL6 melanoma, the prophylactic intravenous (4~100 ㎍/mouse) or oral (2 ㎎/mouse) administration of OeE-100 significantly inhibited tumor metastasis as compared with tumor controls. Peritoneal macrophages stimulated with OeE-100 produced various cytokines such as TNF-α, IL-6 and IL-12. In an analysis of NK-cell activities, i.v. administration of OeE-100 (10~100 ㎍/mouse) significantly augmented the cytotoxicity to YAC-1 tumor cells. Vaccination of mice with boiling-treated tumor cells (BT-vaccine) in combination with OeE-100 (100 ㎍/mouse) showed higher inhibitions in tumor metastasis when compared with the mice of BT-vaccine treatment. In addition, the splenocytes from OeE-100 admixed BT-vaccine immunized mice secreted a higher concentration of Th1 type cytokine such as IFN-γ. These results suggested that the OeE-100 stimulated immune system and was a good candidate adjuvant of anti-tumor immune responses.

The purpose of this study was to identify the effectiveness of a nutrition education program of reducing children`s sugar intake based on social cognitive theory. This education program composed of 6 units was conducted in discretional activity class of the 3rd and 4th grade elementary school students. The results are as follows: First, the rate of correct answers and score of nutrition knowledge significantly increased after nutrition education. The scores of nutrition education in male students, students whose mothers have no job, and students with skinny physiques were much improved after nutrition education(p<0.05, p<0.01, p<0.001, respectively). Second, the dietary attitudes toward reducing sugar intake and checking nutrition labels significantly improved(p<0.05). And also, it tended to decrease in preference and intake of sweet foods. Third, the consumption of candy·caramel·jelly(p<0.05), yogurt(p<0.05), jam·honey(p<0.05), and ice cream(p<0.001) were reduced after nutrition education. Besides, obese students` consumption of isotonic beverages, carbonated beverages, and fruit juice was reduced. Therefore, a positive change in dietary behavior appeared. Based on the above results, we confirmed that the nutrition education program focusing on reducing sugar intake applied with social cognitive theory was effective for improving the nutrition knowledge, dietary attitude and dietary behavior in sugar intake in elementary school students. Especially, this program showed the improvement in nutrition knowledge as well as dietary attitude and behavior in sugar intake among obese children. Since nutrition education during childhood significantly influences lifetime-health and disease prevention, it is necessary to develop theory-based nutrition education program and practice systematic and constant nutrition education in elementary schools.

Early diagnoses of pregnancy for animal such as swine and bovine is extremely important to increase income of a farmhouse and for the management of farm. For the development of immunoasaay system of pregnancy in swine, we report a competitive heterologous enzyme linked immunosorbent assay (ELISA) for the direct measurement of oestrone sulfate (E1S) in diluted urine using anti-E1G (glucuronide) monoclonal antibody which cross react with E1S. The principle of assay was based on the typical solid-phase competitive ELISA methods using E1G-HRP (horseradish peroxidase) as a tracer and E1S for standard. The method had a reasonable sensitivity for the detection of E1S with 0.15 ng/ml as a detection limit. The intra-assay and inter-assay precisions were raging coefficient of from 8.50～9.67% and 8.50～9.87%, respectively, which were quite acceptable. In a field trial with a group 37 sows (18 non-pregnancy and 19 pregnancy sows) after day 29～30 post service, the concentration of E1S were determined to be below 30 ng/ml in all non-pregnancy group and over 48 ng/ml in pregnancy group except one sample. The method described here, heterologous ELISA for the measurement of E1S in urine is good enough for monitoring the early pregnancy test of swine.

5% 에탄올 용액에 인삼, 홍삼, 인삼박, 홍삼박을 가하여 Aceotbacter aceti 3281로 35℃에서 26일간 발효시켜서 인삼식초를 제조하였다. 인삼식초와 홍삼식초의 총당은 0.236㎎/ml 환원당은 0.236㎎/ml, 비중 1.005, 에탄올 함량 0.05%, 균체수 8058CFU/ml, pH .024, 산도 5011을 나타냈다. 물추출 홍삼박과 에탄올 추출 홍삼박의 총당은 1.27㎎/ml 및 1.60㎎/ml, 환원당은 0.077㎎/ml 및 0.727㎎/ml, 비중은 1.001 및 1.004, 에탄올은 모두 0.03%, 균체수는 8051 및 8.1CFU/ml, pH는 2.81 및 2.89%, 산도5.18 및 5.32, 물추출 홍삼박이 4.46, 인삼 및 홍삼은 4.20을 나타냈다. 침출식 식초는 10% 화이트 식초에 인삼, 홍삼, 홍삼박을 단계적으로 가하여 30일간 침출하여 제조하였다. 인삼 추출식초는 0.4∼1.6%, 홍삼 추출식초 0.8% 이상에서, 물추출 홍삼박 식초는 0.8∼1.6%, 에탄올 추출 홍삼박 식초는 0.4∼1.6% 사이에서 기호도가 가장 높았다.

술의 종류에 따른 핵산관련물질의 함량을 비교하기 위해서 HPLC로서 그 함량을 조사하였다. Cystosine의 함량은 앵두술>자두술>동동주>청하>파인애플술>소주의 순서였다. Guanine의 함량은 동동주>청하>파인애플술>자두술>소주의 순서였다. Uridine의 함량은 동동주>청하>앵두술>파인애플술>자두술>소주의 순서였다. Adenine의 함량은 동동주>청하>앵두술>파인애플술>자두술>소주의 순서였다. Guanosine의 함량은 동동주>앵두술>청하>자두술>파인애플술>소주의 순서였다. Adenosine의 함량은 동동주>청하>앵두술>자두술>파인애플술>소주의 순서였다.

Arthrobacter sp. L-3이 생성하는 glucose isomerase를 DEAE-cellulose column chromatography법으로 2단계 NaCl농도 구배로 용출함으로서 순수분리하였다. 이것이 SDS-acrylamide gel electrophoresis상에서 단일띠를 보임으로서 매우 잘 분리되었음을 알 수 있었다. Glucose isomerase의 K_m값과 V_max값이 각각 0.175M, 0.29(㎎/㎖/min)로 얻어졌다. 한편, SDS-acrylamide gel electrophoresis와 Sephadex-G100-50에 의한 gel filtration으로부터 분자량이 각각 42, 000과 180, 000으로 얻어져, 이 효소는 분자량이 42, 500인 4개의 subunit로 구성되었음을 알 수 있었다.

The objective of this study was to propse the way that could enlarge the course in life for the graduates of Department of Food and Nutrition in Junior College. This study was carried out through the analysis of statistical data and questionaries gathered from professors of Department of Food and Nutrition in Junior College and from dieticians of industry, school and hospital. The results of this study could be summarized as follows. 1. The cultivation number of dieticians is too excessive in comparison with the need, and so it is necessary for the colleges to restrict the new establishment of Department of Food and Nutrition for a time, and for the government to tighten the employment system of dieticians. 2. The employment of dieticians will be extended to such fields as school, hospital, public health center and counsel office of nutrition to act as a nutritional educator or a medical server in the future, and consequently the colleges which cultivate dieticians should improve the curriculum to agree with this. 3. It is necessary for the government to renovate the system of dietician arrangement to such a way as establishes a new rule in which additional dietician should be employed in accordance with the feeding number in industry and hospital, as restricts the position-combine of a dietician with a cook in a large feeding institute, as gives the qualification of a nutritional instructor only to the diatician at public health center, as gives the qualification of a nutrition -teacher to the dieticians who completed the course of study for the teaching profession and then allows them to act as a nutrition-teacher in school. 4. The way to give a higher grade of educational opportunity should be given to the graduates of Department of Food and Nutrition in Jonior College for the supplement of dietician`s faculty.