We experimented to see if the tolerance of Agaricus bisporus to Pseudomonas sp. causing the bacterial brown blotch disease which is causing great damage in the button mushroom cultivation was inherited. There was no correlation between mycelial growth rate and mushroom tolerance to pathogens at each temperature. In the mycelial stage, the strains tolerance to Pseudomonas sp. were not as strong as those of their parents, but they were generally stronger or more tolerance than those of weaker strain (ASI1321). In fruiting body, tolerance was decreased compared to mycelial. In fruiting body, later generations of two strains had similar tolerance, unlike strong or weak tolerance of parental generation. Therefore, browning of fruit body is thought to be caused not only by tolerance but also by various other factors. Especially, the future generation of the strains which were tolerance to Pseudomonas sp. were very weak in the mycelial stage, indicating that the tolerance of the parental generation was inherited to the later generations. The damage of each pathogen was different in mycelium and fruiting body. P. tolaasii caused higher browning than P. agarici in fruit body. P. reactans did not have a significant effect on the mycelium but affected the browning of the fruit bodies. P. agarici had higher mycelial growth inhibitory ability than fruiting body.

Information and communication technology(ICT) around the world in the 21st century presents a new vision of agriculture. Time, place, and the high-tech industry to overcome barriers to the fusion of the so-called “smart agriculture” is changing the landscape of agriculture. Precision Agriculture’s core container production for the mushroom cultivation temperature, humidity, irradiation, self-regulation, such as carbon dioxide, the optimal environment for mushroom cultivation was implemented. Auricularia auricula-judae, known as the Jew's ear, wood ear, jelly ear or by a number of other common names, is a species of edible Auriculariales fungus found worldwide. The fruiting body is distinguished by its noticeably ear-like shape and brown colouration. The fungus can be found throughout the year in temperate regions worldwide, where it grows upon both dead and living wood. We controlled different light source (Blue-Red-White combined LED, blue LED, red LED and fluorescent light) with different intensity of LED irradiation (1.5, 10.5 and 20.5 μmol/m2s for LEDs) to compare growth and development. Lights were treated with 12 hour on/ 12hour off cycle maintained in a controlled room with 19 ~ 21oC temperature, 85~95% humidity, and 1000ppm CO2 atmosphere concentration for 30 days. Growth and development differed from the LED color source and intensity of LED irradiation. Growth and development was most effective in 1.5 μmol/m2s irradiation for blue LED. All LED light sources showed less growth and development in highest intensity of irradiation, which indicates that higher than 20.5 μmol/m2s for LED is not effective. After harvesting fruit bodies, we measured their weight, length, width, thickness and chromaticity of fruit bodies. 1.5 μmol/m2s blue LED group was the best result of harvest with average individual weight (5.38g), length (65.37mm), width (56.87mm) and thickness (13.51mm) with fine chromaticity. This results show us that 1.5 μmol/m2s blue LED was the best effect on growth and development of Auricularia auricula-judae mushroom’s ICT system container type environment.

This work aimed to discriminate the geographical origins of Domestic and Chinese Wolfiporia extensa. subjected to of oxygen (45.32 to 48.07%), carbon (38.09 to 40.12%), hydrogen (6.05 to 6.78%), and nitrogen (0.16 to 0.23%). Antioxidant activity was examined by DPPH free radical scavenging activity. According to the results of the experiment, no significant differences were found between domestic (IC50, 7.25 mg/ml) and Chinese (IC50, 8.35 mg/ml) Poria cocos wolf. However, as determined by the inorganic mineral contents were significantly different between domestic and Chinese W. extensa. The amount of potassium in domestic and Chinese W. extensa was 47.60 ± 8.78% and 33.14 ± 17.27%, respectively. The results of this study suggested that the analysis of inorganic components by ED-XRF should be useful for origin identification of Poria cocos wolf.

This study is carried out to compare ingredients and antioxidant activity of Bokryeong, Poria cocos wolf, cultivated in the landfill and facility. Comparisons of the composition of the landfill and facility Bokryeong were conducted in the first and second year. As a result, there was no statistically significant difference in all four components (oxygen, carbon, hydrogen, nitrogen). In the second year, GC-MS analysis was carried out using the extracts containing ginseng epidermis, using the samples taken from the Gangwon-do area in the first year and the second year. As a result, 1,3-Dihydroxyacetone dimer, 2-Furancarboxaldehyde, and Acetaldehyde were detected in the landfill Bokryeong. 2-Furancarboxaldehyde and Acetaldehyde contents were higher in facility Bokryeng. In biological activity assay, The anti-inflammatory of the landfill Bokryeng and facility Bokryeng were compared by using Raw 267.4 cell lines. As a result, it was confirmed that both of them had anti-inflammatory, but no difference was found.

The aim of the study was carried out to investigate a single oral toxicity of a 50% ethanol extract derived from fruiting body of Poria cocus (PCE) using male and female SD rats. Groups consisted of five male and female rats were treated with a single dose of the test substance intragastrically at 0, 5, 50, 300 and 2,000 mg/kg, respectively. Animals were monitored for the mortality and changes in the body weight, clinical signs and gross observation during the 14 days after dosing, upon necropsy. As the results, we could not find any mortality. Compared with the control group, significant weight change was not observed in the experimental group. Also there was no difference in water and food consumption or gross pathological findings at terminal sacrifice among the groups of rat treated with different doses of the test substance. The results suggested that the approximate lethal dose of ACM in both female and male rats were considered as over 5000 mg/kg.

In order to examine the hypolipidemic and hepatic steatosis preventing activities, we investigated mechanism of Auricularia auricula-judae 70% ethanol extract (AAE) in vivo and in vitro. Normal diet (ND) and high fat diet (HFD) with or without 0.1% (w/w), 0.3% (w/w) and 1% (w/w) AAE were given to male C57BL/6 mice. Plasma lipids and liver enzymes were measured and tissue sections of liver were examined. Further mechanistic studies in mouse 3T3-L1 adipocytes were performed in vitro by verifying triglyceride, glycerol, GPDH (glycerol-3-phosphate dehydrogenase) activity and mRNA expression of adipogenic and lipogenic genes using RT-PCR amplification. Body weight and adipose tissue mass were significantly reduced in ND and (HFD plus AAE) fed mice compared to HFD mice. These findings suggest that AAE may reduce the risk of hepatic steatosis by modulating plasma lipids via the regulation of adipogenic/lipogenic transcriptional factors. AAE may have interesting applications to improve plasma lipids and liver enzymes (A part of results have been published in Int J Med Mushrooms).

This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and 35oC, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Other minor components for optimal growth were thiamine-HCl and nicotinamide as vitamins, acetic and lactic acid as organic acids, and MgSO4·7H2O and FeSO4·7H2O as mineral salts. Wolfiporia cocos is a well-known traditional medicine in China, Japan, Korea, and other Asian countries owing to its numerous therapeutic properties. With the aim to determine the morphology and genetic characteristics of W. cocosten strains of W. cocos were cultivated in vitro, and subsequently, rapid amplification of polymorphic DNA was performed. To the best of our knowledge, this is the first study to examine the morphology of fruit bodies of W. cocos in Korea. W. cocos were cultured on PDA agar at different temperatures (12, 16, 20, 24, and 28oC) under 12-hour light (600 Lux) / 12-hour dark photoperiod condition for 1 month. Appearance of fruit body was the highest at 28°C condition in all the strains investigated. Honeycomb-like structure on sclerotia was observed in Andong 01, Andong 02, Andong 03, KFRI 1104, KFRI 1105, KFRI 1106, KFRI 1107, KFRI 1108, and ASI 13007 strains. The KFRI 1103 strain formed cosmos petal-like structure on sclerotia. The average size of basidiospores was recorded as 7.55 μm in height and 3.35 μ in width. This study was carried out to discriminate the geographical origin from Korea and Chinese Wolfiporia extensa. By proximate composition analysis, both were identified as similar, showed oxygen was 45.32-48.07%, carbon was 38.09-40.12%, hydrogen was 6.05~6.78% and nitrogen was 0.16-0.23%. Antioxidant activity was examined by DPPH free radical scavenging activity. No significant differences were found as well for the antioxidant activity between Korean and Chinese product. However, the contents of inorganic components ED-XRF (X-Ray Fluorescence Spectrometer) were significant different in Chinese and domestic W. extensa. The Potassium and iron in Chinese and domestic W. extensa were 47.60±8.78% and 14.5±3.86% as well as 33.14±17.27% and 9.13±4.83%, respectively. From the above resuls, the analysis of inorganic components by ED-XRF may be used for discrimination of the geographical origin of W. extensa.

Diversification to develop oak pruned neck utilization technology reduction of operation cost of cheonma( Gastrodia elata) farmers is the purpose of this research, but the results of the experiment are as follows. The soil chemistry of the plantation was very weak in 2015 when the pH was 5.7, weakly acidic and EC was 0.41 (ds/m), but the faux spring area was very sunny, but at the end of May, early June, late July And so on were at a maximum temperature of 25oC or more, and underground temperature fell below freezing in late January 2016. In the content of the survey of the harvested volume, treatment with a diameter of 6 to 10 cm appeared in 1,366 g total weight and in treated plots with a diameter of 20 cm or more, appeared in 1,542 g, confirming that the number and weight of the interspaces from the small neck is higher than the practice.

Shiitake culture in sawdust is a widely applied method, which can supplement the disadvantages of costly and time consuming oak log cultivation. In sawdust cultivation, browning of surface mycelia is an important stage for the productivity and longevity of sawdust media. Surface browning forms protection coat for the substrate, which can block the invasion of outer pathogens and suppress water evaporation in the substrate. We controlled different light source (red LED, white LED, blue LED, and fluorescent light) with different intensity of illumination (1.5, 10.5, 20.5 μmol/m2s for LEDs and 10, 100, 300 lux for fluorescent light) to induce browning. Lights were treated with 1 hour on/ 1 hour off cycle maintained in a controlled room with 20℃ temperature, 60% humidity, and 1200 ppm CO2 atmosphere concentration for 60 days. Browning effect differed from the source and intensity of illumination. Browning was most effective in 1.5 μmol/m2s for red and blue LED. All light sources showed less browning in highest intensity of illumination, which indicates that higher than 20.5 μmol/m2s for LEDs or 300 lux for fluorescent light are not effective. After harvesting fruit bodies, we measured their weight, length and width of pileus and stipe, chromaticity, and hardness. Treatment with 1.5 μmol/m2s blue LED produced the best harvest with highest average individual weight (21.2g), stipe length (30.8 mm), and hardness (377.9 g) with fine length and width of pileus, and chromaticity. This results indicate that 1.5 μmol/m2s blue LED showed the best browning effect which resulted in the best harvest yield.

Wolfiporia cocos is a widely known traditional medicine in China, Japan, Korea, and other Asian countries due to its various medicinal effect. Aiming to determine the optimum condition for W. cocos fruit body induction, we cultured 10 strains of W. cocos on potato dextrose agar medium (PDA) in different temperature conditions (12, 16, 20, 24, 28oC). KFRI 1105 exceptively formed fuitbody in low temperatures (12 and 16oC), but in other strains, fruit body induction was restricted. in 12 and 16oC. Fruit body formation started 20oC, and formation rate increased proportionally with the temperature increase. 5 strains induced fruit body in 20oC, 7 strains in 24oC, and 9 strains in 28oC. This is the first research to identify a fruit body formation in vitro cultured W. cocos in Korea. This investigation will enable further studies of W. cocos physiology and breeding. In summary : 1. We identified the optimal conditions for W. cocos fruit body induction in the PDA plate. 2. The fruit body was best induced when it was cultured in 28 oC for 32 days. 3. The fruit bodies showed a honeycomb-like structure, and the average spore size was 7.55 μm in height and the average width was 3.35 μm.

Wolfiporia cocos is a well-known traditional medicine in China, Japan, Korea, and other Asian countries owing to its numerous therapeutic properties. With the aim to determine the morphology and genetic characteristics of W. cocosten strains of W. cocos were cultivated in vitro, and subsequently, rapid amplification of polymorphic DNA was performed. To the best of our knowledge, this is the first study to examine the morphology of fruit bodies of W. cocos in Korea. W. cocos were cultured on PDA agar at different temperatures (12, 16, 20, 24, and 28oC) under 12-hour light (600 Lux) / 12-hour dark photoperiod condition for 1 month. Appearance of fruit body was the highest at 28°C condition in all the strains investigated. Honeycomb-like structure on sclerotia was observed in Andong 01, Andong 02, Andong 03, KFRI 1104, KFRI 1105, KFRI 1106, KFRI 1107, KFRI 1108, and ASI 13007 strains of . The KFRI 1103 strain formed cosmos petal-like structure on sclerotia. The average size of basidiospores was recorded as 7.55 μm in height and 3.35 μ in width.

Timing, place, and the high-tech industry to overcome barriers to the introduct of the so-called “smart agriculture”. The core factors of precision agriculture, including temperature, humidity, location information, and real summary information, are all significant for ICT controlling technique. The system is a four-season container allowing mushroom product, humidity, illumination, and factors such as carbon dioxide were regulated to create the optimal environment for mushroom cultivation. Hericium erinaceum (also called Lion's Mane Mushroom, Bearded Tooth Mushroom, or Bearded Tooth Fungus) is an edible and medicinal mushroom in the tooth fungus group. During approximately 40 days of incubation, 1.4 kg of oak sawdust was used as the test medium. The humidity, temperature, and CO2 density were maintained at >85–95%, 18–20oC, and 700–1,000 ppm, respectively, in the container type culture system. The time for primordium formation was observed to be 4–7 days. The amount of fruitbody production was 74–95 g per bag. This technique will be used to establish a new community support program, in conjunction with international movement, domestic sales and exports are also planned.

This study aimed to discriminate the geographical origins of domestic and chinese Wolfiporia extensa. They were subjected to oxygen (45.32 to 48.07%), carbon (38.09 to 40.12%), hydrogen (6.05 to 6.78%), and nitrogen (0.16 to 0.23%). Antioxidant activity was examined by DPPH free radical scavenging activity. According to the results of the experiment, no significant differences were found between domestic (IC50, 7.25 mg/ml) and Chinese (IC50, 8.35 mg/ml) W. extensa. However, as determined by the inorganic mineral contents were significantly different between domestic and Chinese W. extensa. The amount of potassium in domestic and Chinese W. extensa was and 33.14 ± 17.27%, 47.60 ± 8.78%, respectively. The results of this study suggested that the analysis of inorganic components by ED-XRF should be useful for origin identification of W. extensa

Time, place, and the high-tech industry to overcome barriers to the fusion of the so-called "smart agriculture" is changing the landscape of agriculture. Precision Agriculture's core temperature, humidity, location information, and summary information in real time, all significant ICT controlling technique. The system is a four-season container production for the mushroom cultivation temperature, humidity, illumination, self-regulation, such as carbon dioxide, the optimal environment for mushroom cultivation was implemented. Hericium erinaceum (also called Lion's Mane Mushroom, Bearded Tooth Mushroom, or Bearded Tooth Fungus) is an edible mushroom and medicinal mushroom in the tooth fungus group. Native to North America, Europe and Asia it can be identified by its tendency to grow all the spines out from one group (rather than branches), long spines (greater than 1 cm length) and its appearance on hardwoods. About 40 days of incubation 1.5kg oak sawdust was used as the test medium bag. The humidity was maintained more than 85~95% and temperature was 18~20°C, and CO2 density was 500~1,000ppm. The days for primordium formation showed us 6~12 days. The amount of fruitbody production was 110~260g per bag. This technique to establish new community support program in conjunction with the international movement, domestic sales and exports are also planned.

White button mushroom (Agaricus bisporus) is the most widely consumed mushroom in the world, and domestic consumption is increasing owing to expansion of the westernized diet in recent years. Before 2000, most Korean mushroom farmers cultivated foreign varieties, but recently, Korean breeders are developing hybrid strains using molecular breeding tools. To produce a better mushroom cultivar, we evaluated some traits of button mushroom Agaricus bisporus strains. Mycelial growth rate at different culture temperatures was investigated by 25>20>15>30>10oC. Recently developed domestic varieties had superior mycelial growth rate compared to the traditionally grown species. In particular, the ASI1338 strain showed excellent mycelial growth rate at different temperatures. Further, we confirmed that ASI1007, ASI1085, ASI1310, ASI1339 were strongly resistant to bacterial brown blotch, whereas ASI1053, ASI1103, ASI1140, ASI1146, ASI1177, ASI1183, ASI1195, ASI1321, ASI1331, ASI1336 were relatively weakly resistant. The average number of days for 16.5, and that for harvest was 5.7; the average yield was 142.7 g/2-kg bag. Mycelial growth rates at 10, 15, 20, 25oC were similar, but that at 30? was lower. The mushroom yield was highly correlated with the number of days for pinheading and harvest.

This study was carried out to determine the basic mycelial culture conditions for Poria cocos growth. According to colony diameter and mycelial density, suitable media for mycelial growth were Malt yeast extract, Potato dextrose agar, Yeast extract agar, and Yeast malt agar. The optimum temperature for mycelial growth was between 25 and 35oC, and the optimum pH value was between 4 and 7. Carbon and nitrogen sources were fructose and yeast extract. The optimum C/N ratio was about 10 to 1 with 2% glucose. Other minor components for optimal growth were thiamine-HCl and nicotinamide as vitamins, acetic and lactic acid as organic acids, and MgSO4·7H2O and FeSO4·7H2O as mineral salts.

The purpose of this study is to address a current mushroom export situation in Gyeongbuk area for establishingexports strategy, and policy of mushrooms export. Mushroom industry has made a rapid growth over the past 20 years in Korea.Gyeongbuk province exports volume of enoki and king oyster mushrooms account for about half of the country, enoki and totalexports in 2009, and 81%, increased to 14% in 2010 and 2010, the maximum exports (9,415 tons) and the maximum exports(14,840 grand)was recorded. But, enoki and total exports in 2011, and -15%, decreased to -19% in 2012, and increased to 32%in 2013, 1% in 2014. King oyster mushroom to represent the Netherlands in Europe, exports were higher exports to China isnegligible. In the case of China in 2001, 5 spots automated mushroom factory, production, but only 8,000 tons in 2011, 652mushroom factory, production of 1,100,000 tons and in 2012, 788 mushroom factory, production of 1,520,000 tons quicklygrew.

Information and communication technology(ICT) around the world in the 21st century presents a new vision of agriculture. Time, place, and the high-tech industry to overcome barriers to the fusion of the so-called "smart agriculture" is changing the landscape of agriculture. Precision Agriculture's core temperature, humidity, location information, and summary information in real time, all significant ICT controlling technique. The system is a four-season container production for the mushroom cultivation temperature, humidity, illumination, self-regulation, such as carbon dioxide, the optimal environment for mushroom cultivation was implemented. Hericium erinaceum (also called Lion's Mane Mushroom, Bearded Tooth Mushroom, Hedgehog Mushroom, Satyr's Beard, Bearded Hedgehog Mushroom, pom pom mushroom, or Bearded Tooth Fungus) is an edible mushroom and medicinal mushroom in the tooth fungus group. Native to North America, Europe and Asia it can be identified by its tendency to grow all the spines out from one group (rather than branches), long spines (greater than 1 cm length) and its appearance on hardwoods. The humidity was maintained more than 85~90% and temperature was 16~18℃, and CO2 density was 500ppm. The days for primordium formation showed us 7~12 days. The width of pileus was 77~115mm. The length of pileus was 43~72mm. The spine was 11~14mm. The amount of fruitbody production was 125~240g per bag. This technique to establish the agricultural sector of Official Development Assistance (ODA) projects and new community support program in conjunction with the international movement, domestic sales and exports are also planned.

Auricularia auricula-judae is an edible mushroom, which is known as wood ear, free ear, black ear mushroom, and free jelly fish. This study was carried out to obtain the basic information for mycelial culture conditions of Auricularia auriculajudae. According to colony diameter and mycelial density, the media for suitable mycelial growth were PDA and MCM. The optimum temperature for mycelial growth was 25~30oC. Carbon and nitrogen sources were mannose and malt extract, respectively. The optimum C/N ratio was in the range of 10 to 1 with 2% glucose. Other minor components for the optimal growth were thiamine-HCl and biotin as vitamins, succinic acid and lactic acid as organic acids, and MgSO4·7H2O and KH2PO4 as mineral salts.

한국에서의 복령연구는 형태학적 관찰 및 특징, 채집기, 재배방법, 복령균의 배양학적 특성연구 (홍과이, 1990), 1994년 농촌진흥청에서 복령의 인공재배법이 개발되면서 드링크제, 제빵제재 등의 식품화연구도 2000년대 초까지 진행되었다. 복령의 에탄올추출물은 폐암, 난소암, 피부암, 중추신경암, 직장암 세포 성장에 높은 저해활성을 나타내는 등 항암효과 등의 기능성이 입증되었다(권 등, 1998). 하지만 현재까지도 복령의 수요를 천연채집 및 수입에 의존하고 있어서 재배시 고품질, 다수확을 위한 인공재배방법을개선해야할 필요가 있다. 최근에는 경북농업기술원과신농버섯연구소 등에서 지상에서 재배법을 시도하기도 하였다. 또한 최근의 재배사례에서는 부적절한 재배시기를 선택하는 등의 사유로 실패사례를 간혹 볼수 있다. 최근 복령의 주요 수출국인 중국이 수출을제한하여서 지난 4년간 복령가격이 113% 인상되는등 복령의 국내공급량이 절대적으로 부족한 실정이다. 따라서 현재까지 개발된 복령의 지상재배법과 지하재배법의 장점만을 이용한 새로운 방법의 복령인공재배기술의 확립이 요청된다.