Although piglets have been delivered by embryo transfer (ET) with in vitro produced (IVP) embryos and blastocysts, a success rate has still remained lower level. Unlike mouse, human, and bovine, it is difficult to a production of piglets by in vitro fertilization (IVF) because of an inappropriate in vitro culture (IVC) system in pig. Therefore, the present study was conducted to investigate whether minimized exposure time in IVC can improve the pregnancy and delivery rates of piglets. Immediately after IVM, the oocytes were denuded and co-incubated with freshly ejaculated boar semen for 3.5 to 4 hours at 38.5 ˚C under 5% CO2 in air. To avoid long-term exposure to in vitro state, we emitted IVC step after IVF. After that the presumptive zygotes were transferred into both oviducts of the surrogate on the same day or 1 day after the onset of estrus. Pregnancy was diagnosed on day 28 after ET and then was checked regularly every month by ultrasound examination. The 3 out of 4 surrogates were determined as pregnant (75%) and a total of 5 piglets (2 females and 3 males) were delivered at 118.3 ± 2.5 days of pregnancy period. In conclusion, a short-term exposure time may be an important factor in the production of IVP-derived piglets. It can be apply to the in vitro production system of transgenic pig by IVF, cloning, and pronuclear microinjection methods.

A response surface methodology (RSM) was used to evaluate how pH and ionic strength (IS) affect the fate (i.e. size and colloidal stability) of an SC formulation containing the pyrethroid β-cyfluthrin. The response surfaces determined under a range of environmentally relevant conditions were then used to assess the toxicity of the SC formulation of β-cyfluthrin to D. magna. The changes in hydrodynamic diameter (HDD) and colloidal stability as determined by zeta potential measurement were closely related to either or both of the change in pH and IS with the linear factor of IS being the most significant factor affecting those changes. Thus, the concentration of SC formulation of β-cyfluthrin remaining in the water column was dependent on the pH and IS conditions and highest when the colloidal suspension contained small particles or a lack of agglomeration leading to sedimentation of the particles. The toxicity results show correspondingly higher toxicity to D. magna when exposed to the SC formulation of β-cyfluthrin when pH and IS conditions favor formation of either the smallest HDD or most stable colloidal suspensions.

The aim of this study was to set up the optimal exposure condition according to detector type considering image quality (IQ) with radiation dose in chest digital radiography. We used three detector type such as flat-panel detector (FP) and computed radiography (CR), and charge-coupled device (CCD). Entrance surface dose (ESD) was measured at each exposure condition combined tube voltage with tube current using dosimeter, after attaching on human phantom, it was repeated 3 times. Phantom images were evaluated independently by three chest radiologists after blinding image informations. Standard exposure condition using each institution was 117 kVp-AEC at FP and 117 kVp-8 mAs at CR, and 117 kVp-8 mAs at CCD. Statistical analysis was performed by One way ANOVA (Dunnett T3 test) using SPSS ver. 19.0. In FP, IQ scores were not significant difference between 102 kVp-4 mAs and 117 kVp-AEC (28.4 vs. 31.1, p=1.000), even though ESD was decreased up to 50% (62.3 μGy vs. 125.1 μGy). In CR, ESD was greatly decreased from 117 kVp-8 mAs to 90 kVp-8 mAs without significant difference of IQ score (p=1.000, 24.6 vs. 19.5). In CCD, IQ score of 117 kVp-8 mAs was similar with 109 kVp-8 mAs (29.6 vs. 29.0), with decreasing from 320.8 μGy to 284.7 μGy (about 11%). We conclude that optimal x-ray exposure condition for chest digital radiography is 102 kVp-4 mAs in FP and 90 kVp-8 mAs in CR, and 109 kVp-8 mAs in CCD.

This study analyzed the total number of 19,636 patients and radiation technologists, 11,433 of male and 8,203 of female by examined body parts, age, types of detectors, the using contrast enhancement and working condition of the technologists, regular staffs or rotation-duty staffs, based on the K-DOS program distributed by FDA with the DLP value of diagnostic evaluation. The result shows that the effective radiation dose was 0.7mSv~41.7mSv for each region and male patients had more radiation exposure than females. And the amount of exposure was also affected by the types and the method of detectors. Furthermore, the regular staffs took the role of helping the patient to get reduced amount of radiation exposure than rotation duty-staffs. Computed tomography (CT) use has increased dramatically over the past several decades. In this reason, to support the patients and the workers’ health in the field, the hospitals should apply specialized regular working radiation technologist system and manufacturing companies of those CTs should develop low medical radiation exposure devices.

The effects of coating thickness on the delamination and fracture behavior of thermal barrier coating (TBC) systems were investigated with cyclic flame thermal fatigue (FTF) and thermal shock (TS) tests. The top and bond coats of the TBCs were prepared by electron beam-physical vapor deposition and low pressure plasma spray methods, respectively, with a thickness ratio of 2:1 in the top and bond coats. The thicknesses of the top coat were 200 and 500 μm, and those of the bond coat were 100 and 250 μm. FTF tests were performed until 1140 cycles at a surface temperature of 1100 oC for a dwell time of 5 min. TS tests were also done until more than 50 % delamination or 1140 cycles with a dwell time of 60 min. After the FTF for 1140 cycles, the interface microstructures of each TBC exhibited a sound condition without cracking or delamination. In the TS, the TBCs of 200 and 500 μm were fully delaminated (> 50 %) within 171 and 440 cycles, respectively. These results enabled us to control the thickness of TBC systems and to propose an efficient coating in protecting the substrate in cyclic thermal exposure environments.

본 연구에서는 적설하중 산정을 위한 노출계수를 결정 하는데 필요한 기초자료를 제공하기 위하여 각국의 온실 구조설계기준에서 제시된 노출계수들을 비교분석하였고 우리나라의 각 지역별 노출계수를 결정하고 결정방법에 대하여 개선방안을 분석하였으며 연구결과를 요약하면 다음과 같다. 각국의 노출계수 기준을 비교분석한 결과 노출계수에 영향을 미치는 주요인자는 노풍도, 풍속, 바람막이의 유무인 것으로 분석되었다. 또한 일본을 제외한 각국의 기 준을 종합하면 노출계수는 3가지 단계로 구분되며 바람에 완전히 노출되고 바람이 센 지역의 노출계수는 0.8(0.9), 바람에 부분적으로 노출된 지역은 1.0(1.1), 바람 막이가 조밀하게 설치된 지역은 1.2로 나타낼 수 있다. 따라서 온실의 적설하중 산정을 위한 노출계수는 적용의 용이성을 고려한다면 3단계로 구분하여 제시하는 것이 바람직할 것으로 판단된다. ISO 4355기준에 따라 우리 나라 94개 지역에 대한 노출계수를 산정한 결과 대관령 (0.5)과 여수(0.6)를 제외한 모든 지역의 노출계수가 1.0 과 0.8 두 가지로 대별되었다. 우리나라의 내륙지역이 해안지역에 비해 상대적으로 더 큰 강설 확률을 가지며 최대풍속이 5m·s-1 이상인 일수가 더 작은 것으로 분석되 었다. 우리나라의 노출계수는 3단계로 구분하여 해안 지역을 중심으로 한 바람이 강한 지역을 0.8로 하고 내륙 지역은 1.0으로 하며 촘촘한 바람막이가 있는 경우는 일본을 제외한 각국에서 적용하고 있는 값인 1.2로 결정하 는 것이 바람직할 것으로 판단되며, 임계풍속 5.0m·s-1 이상 일 수에 따른 지역별 구체적인 노출계수는 추가적인 연구를 통해 결정할 필요가 있다.

The objective of this study is to investigate the effect of ELF (Extremely Low Frequency) exposure on the health of elementary school students. A total of 103 students 12~13 years old were chosen for the study. The experimental group consisted of 56 students who went to school near an overhead powerline. The ELF exposure and environmental hazard factors were evaluated during a 24-hour exposure period. The body and dwelling characteristics of the students as well as disease occurrence related to the respiratory system and allergies were investigated through a questionnaire. The brain wave and electrocardiogram were also inspected. The exposure of the group exposed while at a school located near a powerline was 6.8 mG (p<0.01). Based on the questionnaire results, neither the body or dwelling characteristics of the two groups were affected by the ELF emitted from powerline. There was no significant difference between the two groups in relation to the rate of occurrence of respiratory diseases such as wheezing, asthma and bronchitis. Although brain waves of the group exposed to ELF were lower than that of the group not exposed to it in terms of absolute power of gamma and beta, there is no significant difference between the health status of the two groups. According to our study, school students who lived near a power line were exposed to higher ELF levels than those away from a power line. The two groups have significant differences inBrain wave and ECG, but this change doesn't mean there is a difference in health status. Finally, our study has a limitation in terms of the number of study subjects and the restricted area examined.

The study conducted statistical analysis of survey on the 239 workers participating in national Tower Crane installation/dismantlement in order to identify work environment and health hazard exposure of Tower Crane workers. Consequently for work related safety status of Tower Crane workers, the number of installation/dismantlement work was the highest at 15-20 times per month, and safety equipments were not arranged at work and they felt highly anxious when boarding on the Tower Crane. Furthermore, it is found that they feel anxious working in the influence of the weather, noise, vibration, wind, the sun ray, and structure characteristic. They have very low satisfaction in the insecureness of workplace and the work environment, and recognizes that their work affect highly on the health. 81.6% of workers have experienced accident at work, the cause of accidents were mainly due to poor work environment, and the health abnormality they have experienced were mostly fatigue and concentration reduction. For job stress, job demand and conflict in relationship were the highest sections. Total 66.5% of workers complained of musculoskeletal disorder related subjective symptom, and back pain patients were the highest of all groups.

PURPOSES: This study investigates the mechanical performance of carbon-capturing concrete that mainly contains blast furnace slag. METHODS: The mixture variables were considered; these included Portland cement content, which was varied from 10% to 40% of the blast furnace slag by weight. The specimens were exposed to different conditions such as high N2 and O2 concentrations, laboratory conditions and high CO2 conditions. Mechanical performances, including compressive and flexural strengths and carbon-capturing depth, were evaluated. RESULTS : The slump, air content and unit weight were not affected significantly by the variation in cement content. The strength development when the specimens were exposed to high purity air was slightly greater than that when exposed to high CO2. As the cement content increased the compressive and flexural strength increased but not considerably. The carbon-capturing capacity decreased as the cement content increased. The specimens exposed in the field for 70 days had flexural strength greater than 3 MPa. CONCLUSIONS : The results indicate that cement content is not an important parameter in the development of compressive and flexural strengths. However, the carbon-capturing depth was higher for less cement content. Even after field exposure for 70 days, neither any significant damage on the surface nor any decrease in strength was observed.

Chironomous is aquatic insect belonging to order Diptera, family Chironomidae. Their larval stage can be found mainly in aquatic benthic environment, hence good model organism to study environmental toxicology assessments and consider as useful bio indicators of contamination of the aquatic environment. In this study, Chironomus Heat Shock Proteins, Cytochrome 450, Glutathione S-transferase, Serine-type endopeptidase gene expressions were compared between polluted field areas (Chironomus plumosus) and under laboratory conditions (Chironomus riparious) to investigate molecular indicators for environmental contaminant stress assessment. Heavy metal (Al, Fe, Mn, Cu, Cr, Zn, Se, Pb, As, Cd) concentrations in sediments collected from three study areas exceeded the reference values. Moreover, HSPs, CYP450 and GST gene expression except SP for C. plumosus showed higher expression than C. riparious gene expression. Similar gene expression pattern was observed in C. riparious that exposed environment waters up to 96 h when compared to C. plumosus exposed to waters that grown in lab conditions. In summary, this comparative gene expression analysis in Chironomous between field and laboratory condition gave useful information to select candidate molecular indicators in heavy metal contaminations in the environment.

The study measured the volatile organic compounds consistency of the child tympanitis patient family. Benzene, the result which analyzes toluene, ethylbenzene, xylene and TVOC consistency, case of personal exposure they were 0.518 μg/m3, 0.909 μg/m3, 1.299 μg/m3, 0.960 μg/m3 and 273.718 μg/m3 respectively, case it appeared with benzene the interior 0.539 μg/m3 and toluene 1.433 μg/m3 and ethylbenzene 1.253 μg/m3 and xylene 1.899 μg/m3 and TVOC 262.132 μg/m3. The consistency of the outdoor benzene, toluene, ethylbenzene, xylene and TVOC appeared respectively with 0.512 μg/m3, 0.474 μg/m3, 1.177 μg/m3, 0.862 μg/m3 and 22.306 μg/m3. Personal exposure, the residential interior, interrelationship analytical result Benzene of outdoor consistency and Ethyl benzene, Xylene and TVOC personal exposures with the interior and outdoor consistency showed the interrelationship which considers(p < 0.01). VOCs consistency which it follows in residential type the detached home > the multi generation house > the consistency price came out with the apartment order, VOCs consistency which it follows construction year possibility the year valence which is built 4 years at once rises the consistency appeared more highly the case where the case is above 4 years than.

To examine the relationship between competition and environmental factors, the germlings of Ascophyllum nodosum (L) Le Jolis and Fucus vesiculosus L. were cultured in monocultures and mixtures of the two species under two different exposure and nutrient levels. Both intra- and inter-specific competition were examined in comparison of the mortality and growth of germlings in monocultures and mixtures of the two species. The mortality of germlings increased with increasing density and emergence periods both in the monoculture and mixtures of the two species, and the mortality of Ascophyllum was significantly higher than that of Fucus both in submerged and emerged treatments. The growth of germlings of both species reduced with increasing density but F. vesiculosus always grew faster than Ascophyllum. The values of log output ratio were more than 0.1, indicating that Fucus 'won' in the competitive battles with Ascophyllum under two nutrient- and air-exposure levels. Log output ratio was greater in high than in low nutrients, indicating that the growth of Fucus is more enhanced than that of Ascophyllum in high nutrients. In the present study, the outcome of inter specific competition between germlings of Fucus vesiculosus and Ascophyllum nodosum was slightly altered by duration of emergence and nutrient concentration, but not to such an extent as to change the outcome.

The objectives of this study were to characterize the factors affecting exposure to the VOCs and NO2 in the vicinity of Gwangyang industrial complex. The VOCs and NO2 levels were measured for residents of an exposure group (industrial area within 5 km) and a control group (15 km farther), respectively using the VOCs and NO2 filter badge as a passive sampler from August to September 2006. The means of indoor, outdoor, workplace and personal exposure levels of benzene were 1.10 ppb, 0.94 ppb, 1.85 ppb and 2.35 ppb respectively in the exposure group. The means regarding toluene for the exposure group were 9.29 ppb indoor, 8.09 ppb outdoor, 14.5 ppb workplace, 14.2 ppb personal exposure. The means regarding ethylbenzene were 4.96 ppb(indoor), 4.45 ppb(outdoor), 6.84 ppb (workplace), 6.10 ppb(personal exposure), and the means regarding xylene were 0.10 ppb(indoor, outdoor), 0.18 ppb(workplace) 0.17 ppb(personal exposure). The means for the indoor, outdoor, workplace and personal exposure level of NO2 were 18.40 ppb, 18.51 ppb, 18.59 ppb, 18.80 ppb respectively in the exposure group. Correlations between personal exposures and workplace concentrations of individual VOCs and NO2 exposures, and each of the microenvironment was statistically significant.

The objective of this study is to safety assessment for human body on the guided missile combustion products. This study is to verify the safety assessment when operating the interior missile ejection take on verify the safety of the human body. During the missile ejection of combustion products, this study is analyzed combustion products. Result are accepted NIOSH and KOSHA of the safe guideline, and 6 exposure gas to the specified values 42% (CO), 22% (CO2), not detected (others) are within minimal exposures criteria of the reference value respectively. Contribution of these results supported that interior missile ejection during combustion products may have been ensured human safely. Therefore, the future for improving the environmental safety of the shooting projectile steel plate round, dust collector, ventilation and other facilities is to improve environmental safety and efficient renovated design needed by target focused areas.

The purpose of this study is to validate and verify a head nose exposure inhalation system for nano particle inhalation toxicity studies. Carbon nano tube(CNT) particles were generated by a chemical vapor deposition(CVD) generator. And purchased single wall carbon nanotubes(SWCNT) and multi wall carbon nanotubes(MWCNT) were generated by an atomizer. CNT particle distribution was measured by Scanning Nano-Particle Spectrometer(SNPS) and Condensation Particle Counter(CPC). Diameter and length of MWCNT generated by CVD were 10~40 nm and 220~20 μm respectively. Particle number concentration of MWCNT generated by CVD were 1.3×105, 4.1×104, 5.6×103#/cc in high, middle, low chamber respectively. Distribution of particles which were less than 100 nm was 45%. Particle number concentration of SWCNT generated by atomizer after magnetic stirring were 8.5×106, 5.3×105, 1.1×104#/cc and after sonication 6.7×106, 4.1×105, 9.5×103#/cc in high, middle, low chambers respectively. Particle number concentration of SWCNT generated by atomizer after magnetic stirring were 6.7×106, 4.6×105, 8.6×103#/cc and after sonication 7.7×106, 5.1×105, 1.3×104#/cc in high, middle, low chambers respectively. We set up head nose exposure inhalation system to conduct a study on nano particle inhalation toxicity. There were sufficient particle number concentrations of nano particles generated in each chamber.