1) 본 보고는 1965년 10월초 수도백엽고병이 격발한 전라남도내 담양읍, 대전면을 위시하여 천변인 영산포, 해안지대인 수포등 현재에서 피해상황과 그 원인 등을 조사해석하여 방제의 자료로 하고자 한 것이다. (2) 이앙기의 한발과 7월의 호우 등으로 인한 일부답의 침수는 본병이 조기다발하기에 알맞는 기상조건이었다. (3) 담양지방에서는 7월초순 호우가 있은 후, 일부 침수답에서 7월중순에 초발을 보게 되었으며, 농민들이 새로운 큰 병해라고 인식이 높아졌다. (4) 이앙기가 빠른 답일수록 피해가 많았다. (5) 침수지구의 피해가 심하였다. (6) 품종간의 차이가 확연하였으며, 특히, 전남풍, 천본욱이 약하고 농림 6호는 강한 편이었다. (7) 평야부가 고지대보다 피해가 많았다. (8) 휴한답이 이모작답에 비하여 피해가 심하였다. (9) 습답, 반습답에 피해가 심한 편이며 건답이 적은 편이었다. (10) 물고부근의 피해가 내부보다 많은 편이다. (11) 점형발생답을 많이 볼 수 있었다. (12) 발병하였을 때의 낙수가 병세를 조장시켰다는 농민이 다수였다.

This study aimed to investigate the effects of soil amendment (heat-expanded clay and active carbon) and planting of Dendranthema zawadskii var. latilobum on the remediation of salt-affected soil and the plant growth under high calcium chloride (CaCl2) concentration. The experimental group comprised treatments including Non treatment (Cont.), heat-expanded clay (H), active carbon (AC), planting (P), heat-expanded clay+planting (H+P), active carbon+planting (AC+P). A 200 mL solution of CaCl2 at a concentration of 10 g·L-1 was applied as irrigation once every 2 weeks. Compared to the Cont., the incorporation of the ‘heat-expanded clay’ amendment decreased electrical conductivity of the soil leachate and cation exchange capacity, whereas the growth of Dendranthema zawadskii var. latilobum was relatively increased. These results suggest that the combination of ‘heat-expanded clay’ amendment and planting will mitigate negative effect of de-icing salts and improve plant growth in salt-contaminated roadside soils.

The study investigates the impact of inflation rate on economic growth to find the best-fit model for economic growth in Vietnam. The study applied Vector Autoregressive (VAR), cointegration models, and unit root test for the time-series data from 1996 to 2018 to test the inflation impact on the economic growth in the short and long term. The study showed that the two variables are stationary at lag first difference I(1) with 1%, 5% and 10%; trace test indicates two cointegrating equations at the 0.05 level, the INF does not granger cause GDP, the optimal lag I(1) and the variables are closely related as R2 is 72%. It finds that the VAR model’s results are the basis to perform economic growth; besides, the inflation rate is positively related to economic growth. The results support the monetary policy. This study identifies issues for Government to consider: have a comprehensive solution among macroeconomic policies, monetary policy, fiscal policy and other policies to control and maintain the inflation and stimulate growth; set a priority goal for sustainable economic growth; not pursue economic growth by maintaining the inflation rate in the long term, but take appropriate measures to stabilize the inflation at the best-fitted VAR forecast model.

The role of money in the modern economy highly determines the intensity and the development of the macroeconomy. The money supply is assumed to be as much as money demand, which reflects the economic character of a country and indicates the growth and development of macroeconomy. In Indonesia, the money supply (M1) is related to the economic dynamics in either the monetary market or the goods market. This research aims at analyzing factors that influence the money supply and to what extent the economic factors affect the money supply in Indonesia. The analysis method used in this research was Vector Autoregressive (VAR) with some variables, such as money supply (M1), interest rate, and Gross Domestic Product (GDP) from the 1st quarter of 2001 until the 1st quarter of 2013. The data collection method was in the form of data compilation from credible sources, such as Bank of Indonesia (BI), Central Bureau of Statistics (CBS), and International Financial Statistics (IFS). To obtain adequate analysis results, several tests were taken, such as unit-root test, Granger causality test, and optimal lag. VAR analysis formulates the correlation among independent variables, so it also sees the study of impulse response and matrix decomposition.

Alcoholic fatty liver disorder has become a frequent health concern worldwide. To investigate the effects of Brassica oleracea (B. oleracea) sprout extract (BOE), the present study was designed with alcoholic fatty liver in the rat. Initially, the effects of BOE on liver parameters were examined. Male rats were divided into five groups. The normal control group was fed the normal diet, and the BOE group was fed the high fat diet and ethanol with/without BOE for 4 weeks. After 4 weeks feeding period, rats were sacrificed and their livers and blood were used for fatty liver-related biomarkers analyses. As a result, BOE ameliorated fatty liver-related enzymes profiles in liver tissues and also reduced blood alcohol concentration in rat model. We demonstrated that BOE protected the high fat diet and alcohol-induced fatty liver in rat model. Furthermore, BOE increased detoxificative abilities against alcohol.

세계적인 장기경기침체 속에서 보다 정확한 물동량 예측은 항만정책 수행에 중요하다. 따라서, 본 연구에서는 부산항 컨테이너 물 동량(수출입화물과 환적화물)을 단변량 모형인 ARIMA 뿐만 아니라 인과관계가 있을 것으로 예상되는 경제규모(한국, 중국, 미국의 국내총생산), 금리수준 그리고 경기변동을 고려한 벡터자기회귀모형과 벡터오차수정모형을 활용하여 추정하고 비교하였다. 측정자료는 2014년 1월부터 2019년 8월까지 월별 부산항 컨테이너 물동량이다. 분석결과에 의하면, 수출입물동량 시계열은 비교적 안정적(stationary)이어서 VAR에 의해 추정하였고 환적화물은 불안정적(non-stationary)하지만, 경제규모, 금리 및 경기변동과 공적분(장기적인 균형관계)를 띠고 있어 VEC모형으로 추정하였다. 추정결과, 안정적인 수출입화물 추정에서는 단변량 모형인 ARIMA가 우수하고 추세가 있는 환적화물은 다변량모형인 VEC모형이 보다 예측력이 우수한 것으로 나타나고 있다. 특히 수출입화물은 우리나라 경제규모와 관련이 있고, 환적화물은 중국과 미국 경제규모와 밀접한 관련이 있다. 또한 중국 경제규모가 미국에 비하여 더 밀접하게 나타나고 있어 환적화물 증대전략에 시사점을 주고 있다.

본 연구는 국내 특산식물이며 신 관상식물로 기대되는 산꼬리풀[Veronica rotunda var. subintegra (Nakai) T.Yamaz.]의 효과적인 재배법을 구명하고자 수행되었다. 플러그 육묘는 파종용기와 파종량을 달리하였다. 재배는 묘의 소질, 식재용기, 토양종류 및 차광정도를 달리하여 처리하였다. 묘의 소질은 162, 200 및 288구 트레이를 이용하여 육묘한 묘를 사용였으며, 파종량은 1, 2, 4 및 6립을 파종하여 생산된 묘를 사용하였다. 본 연구에서 산꼬리풀의 육묘 시 트레이 종류는 162구가 적절하였으며, 셀 당 1립씩 파종할 경우 각 개체의 생육이 증가하였으나 4립 파종 시에는 전체 식물의 생육에 유리하였다. 묘의 소질에 따른 실험은 162구 트레이에서 생산된 묘와 4립씩 파종하여 생산된 묘에서 각기 우수한 생육을 보였다. 산꼬리풀 재배 시 식재용기의 용적량이 커질수록 지상부 및 지하부의 생육이 증가 하는 경향이었으며, 용기의 재질에 따른 유의적인 차이는 나타나지 않았다. 토양조건별 생육은 원예상토에서 가장 왕성하였으며, 혼용토에서는 피트모스에 비해 마사토의 함량이 높은 조건에서 양호한 생육을 나타냈다.

마이크로웨이브 균일분산 시스템을 이용하여 자연생태계에 영향을 주지 않고 잡초종자발아를 제어함으로써 토양 및 수질 오염에 영향을 주지 않는 새로운 개념의 제초제 대안을 제시하고자 한다. 명아주(Chenopodium album var. centrorubrum), 쇠비름(Portulaca oleracea)과 한련초(Eclipta prostrata)의 식물에 대한 마이크로웨이브의 토양 함수량별, 깊이별 조사한 결과 균일 분사된 마이크로웨이브를 이용한 잡초 종자의 발아율 측정에서 마이크로웨이브는 토양 함수량에 따른 종자의 발아 조절에는 토양 함수량 20%, 30%에서 최고의 효율이 나타났으며, 토양의 깊이에는 별 영향을 받지 않는 것으로 나타났다. 마이크로웨이브 조사시간은 30초 이상 조사시 잡초 종자의 발아를 억제 할 수 있는 것으로 나타나 적절한 토양 함수량(20~30%) 및 이에 따르는 30초 이상 조사한 마이크로웨이브의 균일분산 시스템을 통해 제초제를 대신하여 잡초종자발아를 조절할 수 있을 것으로 생각된다.

Background: This study was conducted to investigate the changes in the photosynthetic parameters, chlorophyll content, chlorophyll fluorescence, and growth characteristics of Aruncus dioicus var. kamtschaticus seedlings under different shading treatments. Methods and Results: The shading treatment was regulated with the shading level (non-shaded, 35%, 55%, and 75% shading). Photosynthetic activities, such as net photosynthetic rate, stomatal conductance, stomatal transpiration rate, and performance index on absorption basis (PIABS)were the highest under 35% shading (4.36 μ㏖ CO2·m−2·s−1, 54.2 m㏖ H2O·m2·s−1, 0.66 m㏖ H2O·m−2·s−1, and 1.3, respectively), and the lowest under 75% shading. This implies that the decrease in net photosynthetic rate may be due to an inability to regulate water and CO2 exchanged through the stomata. Thechlorophylla, b, and a + b contents were increased with elevating shading level and the chlorophyll a/b ratio showed non-significant differences. It was found that the dry weight (leaf, shoot, and whole) was the highest (1.14 g, 0.49 g, and 2.31 g, respectively) under 35% shading and the t/R ratio was the highest under 75% shading. Conclusions: It is concluded that 75% shading exhibited a strong reduction of photosynthetic activity, and 35% shading showed the best conditions for the early growth and cultivation of A. dioicus var. kamtschaticus.

본 연구에서는 당근 지상부 추출물 및 용매 분획물의 항염, 항균 활성을 확인하고 유효성분을 분리하여 화학구조를 동정하였다. 당근 지상부 에탄올 추출물 및 용매 분획물의 항염 활성을 측정하기 위해 LPS로 자극된 RAW 264.7 세포를 이용하여 NO 생성 억제 활성을 확인하였다. 그 중 에틸아세테이트 분획물이 NO의 생성을 농도 의존적으로 감소시키는 효과가 나타났고, 추가적인 항염 기전 연구를 위해 에틸아세테이트 분획물에 대해 PGE2, 전염증성 사이토카인의 생성량 및 iNOS, COX-2 단백질의 발현량을 측정하였다. 그 결과, 에틸아세테이트 분획물이 PGE2, IL-1β, IL-6의 생성을 감소시키고, iNOS, COX-2 단백질의 발현도 억제 시키는 효과가 있음을 확인하였다. 또한 표피포도상구균과 여드름균을 이용한 활성 실험 결과, 헥산 및 에틸아세테이트 분획물에서 항균 활성이 나타났다. 이와 같은 결과를 바탕으로 에틸아세테이트 분획물에 대해 컬럼 크로마토그래피를 수행하여 3개의 화합물을 분리하였으며, 1H 및 13C NMR 데이터 분석과 문헌을 통하여 화학구조를 동정하였다; diosmetin (1), disomin (2), cynaroside (3). 분리된 화합물 1-3에 대해 항염 및 항균 활성을 측정하였으며, 그 결과 화합물 1-3 모두 NO의 생성을 저해시키는 효과가 있음을 확인하였다. 또한 화합물 3은 여드름균에 대해 항균 활성이 우수하였다. 이상의 연구 결과를 바탕으로 당근 지상부 추출물을 이용한 항염 및 항균 효과를 갖는 천연 화장품 소재로의 개발이 가능할 것이라 사료된다.

Background : Adenophora triphylla var japonica is a perennial herb that belongs to Campanulaceae. Radix Adenophorae is a dried rhizome of A. triphylla and same genus plant. It has contains chemicals such as cycloartenyl acetate, lupenone, β-sitosterol, taraxerone, octacosanoic acid, and praeruptorin A. Radix Adenophorae considered to be effective regulating humoral and cellular immunity, antimutation, restraining adenocarcinoma cell, strengthening cardiac function, allaying a fever, and easing pain and cough. In this study, we tried to establish a mass production system of A. triphylla which has high economic value as a medicinal herb by plant tissue culture in order to cultivate standard varieties. Methods and Results : In this study, A. triphylla internode was used as a explant and it was surface sterilized by 1% sodium hypochlorite for 5 minutes, consequently several times washed with ddH2O. Further it was placed in to MS medium including with axillary bud. The 1/2MS, B5, SH was used in this research. And the plant growth regulator of 0.1 - 2 ㎎/ℓ auxins (NAA, IBA) and cytokines (BA) were used respectively to achieve multiple shoots. The whole study was carried out in the department of Herbal crop research, Eumseong, RDA. Conclusion : In this study we obtained, 6.2 multi-shoots per an explant, and the shoot growth was also favorable in the presence of 1.0 ㎎/ℓ BA and 1.0 ㎎/ℓ IBA.

Background : Acanthopanax divaricatus var. is a Korean woody natural plant which can grow in the field placed in low altitude. Cutting is more efficient for propagation than seedling since it takes 3-years to harvest seeds and get seedling. This study was carried out to find out efficient cutting propagation method. Methods and Results : Hardwood cutting (April 10) and semihardwood cutting (September 7) was performed to accelerate cutting propagation rate on 5 type nursery box soil composed of Sand, vermiculite, peat moss, perlite, and perlite + peat moss. In order to verify growth regulators on cutting propagation, rooton, IBA 2,000 ppm, IBA 3,000 ppm, NAA 500 ppm, NAA 1,000 ppm were treated and cutting performed in sand nursery box soil on March 30 and September 7 with 3 replications of 50 plants. In terms of rooting traits of cutting in April 10 according to nursery box soil, rooting rate was higher as 67% in sand and lower as 11% in peat moss. Root length, root number, and root weight was also higher in sand. In terms of rooting traits of cutting in September 7, rooting rate was higher as 60% in sand and root number was higher in sand and perlite. Root weight was higher in perlite. The result of growth regulator effect on cuttings was as follows. Rooton increased rooting rate higher as 68.7% in cutting on March 30 and IBA 3,000 ppm also increased higher root length, root number, and root weight as 5.4 ㎝, 12.3 ea/plant, 3.13 g/10plant respectively. rooton increased rooting rate higher as 67.3% in cutting on September 7 as same as cutting on March 30 with no significant difference and root length, root number, and root weight were all higher in rooton treatment too. Conclusion : The result of cutting treatment in 5 type nursery box soil on April 10 and September 7 to accelerate cutting propagation rate of A. divaricatus var. shows that sand was most efficient with higher root length, root weight, and rooting rate. Most efficient growth regulator for rooting was rooton with higher rooting rate and better rooting traits altogether same in cuttings on March 30 and September 7.

In this study, a preliminary evaluation of the antioxidant and anti-inflammatory activity of the Ficus erecta var. sieboldii (Miq.) King (FES) leaf extract has been performed to assess its potential as a natural resource for food and medicinal materials. FES was extracted using 70% EtOH and then fractionated sequentially using n-hexane, CH2Cl2, EtOAc, and n-BuOH. To screen for antioxidant and anti-inflammatory agents effectively, the inhibitory effect of the FES extracts on the production of oxidant stresses (DPPH, xanthine oxidase, and superoxide) and pro-inflammatory factors (NO, iNOS, COX-2, PGE2, IL-6, and IL-β) in the murine macrophage cell line RAW 264.7 activated with lipopolysaccharide (LPS) was examined. Among the sequential solvent fractions of FES, the CH2Cl2 and EtOAc fractions showed decreased production of oxidant stresses (DPPH, xanthine oxidase and superoxide), and the hexane and CH2Cl2 fractions of FES inhibited the production of pro-inflammatory factors (NO, iNOS, COX-2, and PGE2). The CH2Cl2 fraction also inhibited the production of pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β). These results suggest that FES has a significant effects on the production of oxidant stresses and pro-inflammatory factors and may be used a natural resource for antioxidant and anti-inflammatory agents.

Background : Spiraea prunifolia var. simpliciflora (Rosaceae) called “Brial wreath” is a deciduous latifoliate shrub that is widely distributed throughout in Northeast Asia. Phytochemical and biological investigation of S. prunifolia have led to the discovery of biologically active compounds. Pharmacological studies revealed that the extract of the root of S. prunifolia possess antioxidant, antipyretic and anti-inflammatory activities. Some chemical constituents such as sterols, phenolics, terpene and fatty acid, as well as ethanolic extracts from the roots of S. prunifolia, have previously been reported to modulate the deleterious effects of diabetes, to prevent high-fat diet-induced obesity, and to prevent cisplatin-induced nephrotoxicity. Our continuing research was carried out to search for other phytochemical constituents from the leavess of S. prunifolia. The chemical structures of compounds were determined by NMR and FAB/MS spectroscopic data interpretation. Methods and Results : Multiple-preparative liquid chromatography (MPLC) purifications were carried out on YMC LC-forte/R instrument (YMC Kyoto, Japan) equipped with YMC-Pack ODS-A columns (ODS gel : 5 ㎛, 10 ㎜ × 250 ㎜). High-performance liquid chromatography (HPLC) was performed on Agilent Technologies instrument (Aglient Tec., Santa clara, CA, USA) equipped with YMC–Pack Pro C18 columns (ODS gel : 5 ㎛, 4.6 ㎜ × 250 ㎜). Next, quantitative analysis was carried out on UPLC-QqQ/MS 3200 Q-TRAP instrument (AB SCIEX Toronto, Canada) using a ACQUITY UPLC (waters corp.) with an ACQUITY BEH C18 column (2.1 ㎜ × 100 ㎜, 1.7 ㎛). The metabolite samples was applied to preparative reversed-phase HPLC and UPLC using gradient method, solvent A [water + 0.1% formic acid (v/v)] and solvent B [acetonitrile + 0.1% formic acid (v/v)]. Conclusion : In this study, we isolated the major metabolites from the stem of Spiraea prunifolia var. simpliciflora by using MPLC and HPLC. UPLC-QqQ/MS was also used to quantify target compounds. Finally, we established methodology and performed the quantitative analysis on target compounds from the stem of Spiraea prunifolia var. simpliciflora.

Background : Osteoarthritis is an inflammatory disorder related with oxidative stress and apoptosis leading to cartilage damage. Recently, Cirsium japonicum var. maackii (CJM) was reported to play a protective role in various inflammatory response. However, the role of CJM in cartilage degeneration and osteoarthritis progression is still unknown. Therefore, this study aims to investigate the protective effect of CJM in a animal model of osteoarthritis and cartilage degradation. Methods and Results : First, in order to determine active ingredient contents of CJM, we were carried out total polyphenolic content and total flavonoid content analyses. As a result, dried aerial parts of CJM were found to contain 149.2 ± 24.1 ㎎·GAE/g dry weight and 27.9 ± 2.0 ㎎·NE/g dry weight in boiling water extraction. Also, the HPLC analysis of CJM showed major compounds identified as cirsimarin and cirsimaritin. In addition, CJM protected against osteoarthritic cartilage destruction in an osteoarthritis mouse model induced by destabilization of the medial meniscus, as demonstrated by histopathological analysis. Conclusion : The results of this study demonstrate that CJM may protect against the osteoarthritis and cartilage destruction. Further study is needed to identify the mechanism for their improvement effects of osteoarthritis and cartilage destruction.

Background : Seed germination for Adenophora triphylla var. japonica Hara. is not good when grown in direct seeding. This experiment was conducted to identify seedling box suitable for seedling transplantation and cultivation to improve farm income and public health. Methods and Result : To investigate the effect on growth, sizes of seedling box used were as follows (width × length × height, in ㎝); 2.5 × 2.5 × 4 (custom), 4 × 4 × 4, 4.5 × 4.5 × 6, 5.0 × 5 × 9.6, 5 × 5 × 11.2, 5 × 5 × 17, and for sowing in narrow strips; 33.5 × 55 × 13, 33.5 × 49 × 23, 33.5 × 49 × 30. The weight of the ground part decreased as box height became lower, highest at 17 ㎝ tall (0.65 g) and lowest at 4 ㎝ (0.23 g). In terms of root length of the seedlings, the longest was 9.0 - 9.3 ㎝ at box height of 17 ㎝ and decreased as height decreased. Root weight increased as box height decreased, the highest at 4 ㎝. After the seedlings were planted, more than 90% survived in seedling box compared to the 50% rooting rate on sown in narrow strips where root net formation did not occur. During harvesting period, plant height was 15.8 - 17.5 ㎝ with a shoot diameter of 13.3 - 20.7 ㎜, leaf length of 5.6 - 7.9 ㎝ and leaf numbers 15.6 - 22.3. Moreover, leaf width was the widest at 8.6 ㎝ at 6 ㎝ box height while dry weight was the most at 6.7 g at 11.2 ㎝. Survival rate and growth characteristics of the ground part were lower in seedling box for sowing in narrow strips. For the underground characteristics, root number increased as box height decreased while dry weight increased as box height increased, with 1.7 - 3.5 and 6.2 - 6.8 less in seedling box for sowing in narrow strips, respectively. The yield in a 17 ㎝ high seedling box was 152 ㎏ per 10 a, 16% higher than conventional level, and decreased as box height decreased. Conclusion : These results suggest that the optimum seedling box height is 11.2 - 17 ㎝ to have lower root numbers and higher dry weight and yield.

This study was conducted to investigate the growth and physiologycal activitis of Aruncus dioicus var. kamtschaticus (Maxim.) H. Hara under the NaCl treatment conditions (0, 100, 200, 300 mM). After 30 days treatment, the growth and physiologycal activitis were investigated. In the growth of plants, the plant height, leaf width, leaf length and ion level were reduced at NaCl treatments of more than 100 mM. The total polyphenol content was decreased by NaCl in a concentrationdependent manner compared to the control group. The contents of total flavonoids did not show any difference at the concentration of 200 mM and 300 mM. However, the content of total flavonoid decreased compared with that of control. In antioxidant activity, the DPPH and ABTS radical scavenging activity and reducing power activity were decreased by NaCl concentration compared to the control. When changes in the content of NO production was monitored by ELISA, production inhibitory effect was 94.5%, 70%, 63%, 56.9% in NaCl concentration of 0, 100, 200, 300 mM, respectively. The growth, ion level, antioxidant and anti-inflammation activity of Aruncus dioicus var. kamtschaticus was reduced at NaCl treatments of more than 100 mM.

Background: Adenophora triphylla var. japonica (Regel) H. Hara shows vegetative growth with radical leaves during the first year and shows reproductive growth with cauline leaves and bolting during the second year. In addition, the shape of the plant varies within the same species. For this reason, there are limitations to classifying the species by visual examination. However, there is not sufficient genetic information or molecular tools to analyze the genetic diversity of the plant. Methods and Results: Approximately 34.59 Gbp of raw data containing 342,487,502 reads was obtained from next generation sequencing (NGS) and these reads were assembled into 357,211 scaffolds. A total of 84,106 simple sequence repeat (SSR) regions were identified and 14,133 primer sets were designed. From the designed primer sets, 95 were randomly selected and were applied to the genomic DNA which was extracted from five plants and pooled. Thirty-nine primer sets showing more than two bands were finally selected as SSR markers, and were used for the genetic relationship analysis. Conclusions: The 39 novel SSR markers developed in this study could be used for the genetic diversity analysis, variety identification, new variety development and molecular breeding of A. triphylla.

Background: In this study, we evaluated the anti-cancer activity and potential molecular mechanism of 70% ethanol extracts of the root of Aralia cordata var. continentalis (Kitagawa) Y. C. Chu (RAc-E70) against human colorectal cancer cells. Methods and Results: RAc-E70 suppressed the proliferation of the human colorectal cancer cell lines, HCT116 and SW480. Although RAc-E70 reduction cyclin D1 expression at the protein and mRNA levels, RAc-E70-induced reduction in cyclin D1 protein level occurred more dramatically than that of cyclin D1 mRNA. The RAc-E70-induced downregulation of cyclin D1 expression was attenuated in the presence of MG132. Additionally, RAc-E70 reduced HA-cyclin D1 levels in HCT116 cells transfected with HA-tagged wild type-cyclin D1 expression vector. RAc-E70-mediated cyclin D1 degradation was blocked in the presence of LiCl, a GSK3β inhibitorbut, but not PD98059, an ERK1/2 inhibitor and SB203580, a p38 inhibitor. Furthermore, RAc-E70 phosphorylated cyclin D1 at threonine-286 (T286), and LiCl-induced GSK3β inhibition reduced the RAc-E70-mediated phosphorylation of cyclin D1 at T286. Conclusions: Our results suggested that RAc-E70 may downregulate cyclin D1 expression as a potential anti-cancer target through GSK3β-dependent cyclin D1 degradation. Based on these findings, RAc-E70 maybe a potential candidate for the development of chemopreventive or therapeutic agents for human colorectal cancer.