숙주나물과 콩나물은 blanching 후 변질 속도가 크게 차이가 나는 바 이들이 Bacillus subtilis, Bacillus cereus, Pseudomonas fluorescens, Listeria monocytogenes 등 4종의 부패성 및 식중독 미생물의 중식에 미치는 영향을 조사하였다. 숙주나물과 콩나물 조리과정 중 blanching 조건이 달라 각각의 조건에서 두 시료 모두 blanching하여 생균수를 측정한 결과 blanching 한 후 미생물수는 저장시간에 따라 증가하나 그 차이가 없었다. 최소배지에 숙주나물과 콩나물 농축물 및 부패성 미생물을 접종한 후 생균수를 측정한 결과 숙주나물에 접종한 모든 균들의 초기 균수가 높았으나 24시간 이후 비슷한 증식양상을 나타내었다. 그러나 숙주나물과 콩나물의 여액을 배지로 사용하여 실험한 결과 미생물의 증식 양상은 균주마다 다른 것으로 나타났다. 저장기간 동안 색도를 측정한 결과 숙주나물의 경우 24시간에는 색차가 거의 없다가 48시간 이후 현저한 차이를 보였고, 콩나물의 경우 24시간에 다른 계통의 색으로 변하다가 48시간 이후 현저하게 많은 차이를 보여 다른 양상을 나타내었다.

This study was conducted to standardize the cooking methods of Omija-Pyuns and to investigate the effect of different starch densities (6% 7%, 8%, 9%) on the texture and taste of Omija-Pyuns. The results of these studies were as follows: 1) It was better for Omija to soak for 18 hours. The most favorite Mungbean starch density of 7% of Omija-Pyuns has the degree of lightness of 18.90, the degree of redness of 19.18, and the degree of yellowness of 3.98. 2) The Brix of syrup used for Omija-Pyuns was 49.4%. 3) As a result of the measurement of texture through Instron, if Mungbean starch density is increased, its hardness also increase. Each of the Omija-Pyuns (6%, 7%, 8%, 9%, in density) has showed its effection (p<0.05). 4) The result of the sensory evaluation showed that the Omija-Pyuns produced with Mungbean starch density of 7% was most preferred in appearance and overall taste, but it did not show the statistical effection (p<0.05) from the Omija-Pyuns produced with Mungbean starch density of 8%. The flavour was highest in Omija-Pyuns of 6%, and as the density was increased, the flavour decreased.

Soybean and mungbean sprouts are vegetables indigenous to Korea which have been grown throughout the year from the most remote age. They had been called interchangeably as duchaeah or duah. Control method of environmental conditions for soybean or mungbean sprouts growing had been already appreciated from long time ago. A growing method once developed might have been handed over considerable period. Siroo (시루) and yongsoo (용수) had been the most common containers and the watering skill is of the utmost importance for soybean sprouts growing. As the demand of soybean sprouts in the market increased, a pit has been used for bean sprouts growing, and transformed into regular soybean sprouts factory. Now bean sprouts are international food and the demand of bean sprouts seems permanent in Korea. Accordingly the long-term policy concerned with the production of soybean sprouts should be prepared, placing emphasis on improved quality and safety.

A mungbean variety ‘Suhyeon’ (Vigna radiata (L.) Wilczek) was derived from the cross between ‘Jeonnam14’ and ‘IT208777’ at the Jeollanamdo Agricultural Research and Extension Services (JARES) in 2010. ‘Suhyeon’ has erect growth habit, heart leaflet, green hypocotyl, black and straight pod when matured, and dull green seed surface. The stem length of ‘Suhyeon’ was 66 ㎝, which was 5 ㎝ less than check variety ‘Owool’. The pods number per plant of ‘Suhyeon’ was 24, which was 5 more than check variety. The sprout yield ratio of ‘Suhyeon’ was 8.67 times, which was 16% more than check variety. The contents of starch, protein and fatty acid of seed were 62.7, 27.3, 1.89%, respectively. The average yield of ‘Suhyeon’ was 1.74 ton/㏊, which increased 8% more than that of check variety. (Variety registration number: 5107)

A mungbean cultivar ‘Sanpo’ (Vigna radiata (L.) Wilczek) was derived from the cross between SM9710-3B-7 and Jangannogdu at the Jeollanamdo Agricultural Research and Extension Services (JARES) in 2012. ‘Sanpo’ has erect growth habit, green hypocotyls, heart leaflet, dull green seed surface, and black and straight pod when matured. The stem length of ‘Sanpo’ were 71㎝, which was 5㎝ longer than check cultivar ‘Owool’. The pods number per plant, the seeds number per pod, and the seed weight of ‘Sanpo’ are similar to check cultivar. It has field resistance to cercospora leaf spot, mungbean mottle virus, and powdery mildew. In addition, ‘Sanpo’ has high lodging resistance. The hard seed rate of ‘Sanpo’ was 2.4%, which was 0.8%point lower than check cultivar. The sprout yield ratio of ‘Sanpo’ was 8.26 times, which was 10% (77%point) more than check cultivar. The average yield of ‘Sanpo’ was 1.62ton/㏊, which was 14% more than the check cultivar ‘Owool’. (Cultivar registration number: 5250)

Mungbean (Vigna radiata) is a fast-growing, warm-season legume crop that is primarily cultivated in developing countries of Asia. We constructed a draft genome sequence of mungbean to facilitate genome research into the subgenus Ceratotropis and to enable a better understanding of the evolution of leguminous species. The draft genome sequence covers 80% of the estimated genome, of which 50.1% consists of repetitive sequences. In total, 22,427 high confidence protein-coding genes were predicted. Based on the de novo assembly of additional wild mungbean species, the divergence of what was eventually domesticated and the sampled wild mungbean species appears to have predated domestication. Moreover, the de novo assembly of a tetraploid Vigna species (Vigna reflexo-pilosa var. glabra) provided genomic evidence of a recent allopolyploid event. To further study speciation, we compared de novo RNA-seq assemblies of 22 accessions of 18 Vigna species and protein sets of Glycine max and Cajanus cajan. The species tree was constructed by a Bayesian Markov chain Monte Carlo method using highly confident orthologs shared by all 24 accessions. The present assembly of V. radiata var. radiata will facilitate genome research and accelerate molecular breeding of the subgenus Ceratotropis.

The ubiquitin conjugating enzyme E2 (UBC E2) mediates selective ubiquitination, acting with E1 and E3 enzymes to designate specific proteins for subsequent degradation. In the present study, we characterized the function of the mung bean VrUBC1 gene (Vigna radiata UBC 1). RNA gel-blot analysis showed that VrUBC1 mRNA expression was induced by either dehydration, high salinity or by the exogenous abscisic acid (ABA), but not by low temperature or wounding. Biochemical studies of VrUBC1 recombinant protein and complementation of yeast ubc4/5 by VrUBC1 revealed that VrUBC1 encodes a functional UBC E2. To understand the function of this gene in development and plant responses to osmotic stresses, we overexpressed VrUBC1 in Arabidopsis (Arabidopsis thaliana). The VrUBC1-overexpressing plants displayed highly sensitive responses to ABA and osmotic stress during germination, enhanced ABA- or salt-induced stomatal closing, and increased drought stress tolerance. The expression levels of a number of key ABA signaling genes were increased in VrUBC1-overexpressing plants compared to the wild-type plants. Yeast two-hybrid and bimolecular fluorescence complementation demonstrated that VrUBC1 interacts with AtVBP1 (A. thaliana VrUBC1 Binding Partner 1), a C3HC4-type RING E3 ligase. Overall, these results demonstrate that VrUBC1 plays a positive role in osmotic stress tolerance through transcriptional regulation of ABA-related genes and possibly through interaction with a novel RING E3 ligase.

The objective of this study was to evaluate the quality characteristics of mungbean starch gels that were prepared with different concentrations (0, 0.5, 1.0 and 1.5%) of peach seed powders (PSP), which exhibit antioxidant characteristics. Mungbean starch gel samples were prepared and subjected to quality characteristics such as moisture content, general components, color, texture profile analysis, total polyphenol contents, DPPH radical scavenging activity, and sensory qualities. The L-value and the a-value for color decreased significantly, whereas the b-value increased significantly as the PSP concentration increased (p<0.05). In the texture profile analysis, the mungbean starch gel with 1.5% PSP showed significantly lower degrees of hardness, cohesiveness, springiness, gumminess and brittleness (p<0.05). The total polyphenol content was highest in the 1.5% PSP to which mungbean starch gel was added, which also showed a higher than 70% DPPH radical scavenging activity level in a dose-dependent manner (p<0.05). In the sensory test of the mungbean starch gel, its sensory scores for flavor and taste were highest in the 0.5% PSP to which mungbean starch gel was added.

A low temperature-inducible cDNA designated as VrUBC1 from mungbean (Vigna radiata) was isolated by subtractive hybridization method. By rapid amplification of cDNA end technique, the full-length cDNA of VrUBC1 was obtained. The full-length cDNA of VrUBC1 contains an open reading frame of 444 nucleotides in length and capable of specifying a 16.5-kDa protein of 148 amino acids (aa) with an isoelectric point of 7.72. VrUBC1 mRNA was induced by NaCl and ABA, but not by wounding and low temperature stress. It was shown that VrUBC1-GFP was localized to the cytoplasm in tobacco cell. To examine the function of VrUBC1, VrUBC1 was expressed in Escherichia coli as His-fusion protein. Purified VrUBC1-His recombinant protein was shown to have ubiquitination activity in vitro. For the in vivo functional analysis of VrUBC1, VrUBC1 was expressed in yeast ubc4/5 double mutant. Stress tolerance was tested in the VrUBC1 overexpressing Arabidopsis transgenic plants. We propose that VrUBC1 play an important role in protein degradation processes during abiotic stress in plants.

본 연구는 남부지역에서 생력재배로 일시수확 및 관행재배로 생산한 녹두의 품질을 구명하고자 수행하였으며 결과는 다음과 같다. 생력재배는 7월 23일에 파종하여 10월 28일 일시에 수확하였고, 관행재배는 6월 13일에 파종하여 8월 중순부터 3차례 손으로 수확하였다. 1. 종실의 전분, 조단백질, vitexin 및 isovitexin 함량은 재배방식과 수확기에 따른 유의차가 없었다. 종실의 조지방 함량은 생력재배, 관행재배 3차, 2차, 1차 수확한 종실 순으로 많았다. 2. 종실의 포화지방산과 불포화지방산의 조성비는 재배방식 및 수확기에 따른 차이가 없었다. 지방산 종류는 관행재배로 2차 수확한 종실에서 17종으로 가장 많았고, 관행재배 3차나 생력재배로 일시에 수확한 종실은 12종으로 가장 적었다. 주요 포화지방산 중에서 palmitic acid와 arachidic acid는 관행재배 1차, 2차, 3차 및 생력재배 수확 순으로 높은 비율인 반면 stearic acid는 반대의 경향이었다. 주요 불포화지방산 중에서 linoleic acid는 관행재배 1차, 2차, 3차 수확 및 생력재배 순으로 조성 비율이 높았다. 3. 종실의 아밀로그램 특성은 재배방식 및 수확기에 따라 큰 차이를 나타냈다. 관행재배로 1차에 수확한 종실은 호화개시온도, 최고점도, 최종점도, 최저점도 및 강하점도가, 관행재배 3차에 수확한 종실은 치반점도가 유의하게 높았다. 반면에 생력재배로 수확된 종실은 모든 아밀로그램 측정값이 현저하게 낮았다. 4. 녹두나물을 생산하고 나물의 수율과 색도 및 아미노산 함량을 측정한 결과, 생력재배로 생산한 녹두는 나물의 생산수율이 낮으나 색도와 아미노산 함량은 관행재배로 수확한 종실을 이용하여 생산한 나물과 비슷하였다.

나물용 녹두 신품종 '주현'은 금성녹두와 VC1834-4-B-2-B-3B-B를 인공교배하여 2009년에 육성되었다. '주현'의 생육습성은 직립, 잎 모양은 심장형, 백축색은 녹색, 성숙기에 꼬투리는 검고 곧으며, 종피는 광택이 없는 녹색이다. 개체당 협수는 26개로 표준품종인 '어울녹두'보다 8개 많고, 녹두나물 수율은 표준품종보다 18% 많다. '주현'은 바이러스, 갈색무늬병, 흰가루병 및 도복에 대한 포장저항성은 강하고, 평균수량은 1.84 MT/h