Obesity is a pro-inflammatory state that contributes to the development of metabolic disorders such as hyperlipidemia, insulin resistance, type 2 diabetes, non-alcoholic fatty liver, and cardiovascular disease. In this study, we evaluated the inhibition of adipogenesis in 3T3-L1 cells and in high-fat diet (HFD)-induced obese mice by Peucedanum japonicum Thunberg L. water extract (PJT). Lipid accumulation measurement indicates that PJT markedly inhibited adipogenesis in a dose-dependent manner. RT-PCR results demonstrated that the mRNA expression of adipogenic transcription factors such as peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer binding protein- α (C/EBPα) in 3T3-L1 cells were significantly down-regulated by PJT treatment. Oral administration of PJT (100, 300, and 500 ㎎/㎏, b.w/daily for 4 weeks) was conducted in high-fat diet induced obese mice and C57BL/6 mice. The PJT-administered group of HFD-induced mice had a lower body weight gain, along with decreased serum levels of glucose, triglycerides, and total cholesterol compared with the control mice, however, the HDL-cholesterol/total cholesterol ratio was increased. Furthermore, the elevated mRNA expression levels of adipogenesis related genes in the white adipose tissue of obese mice were significantly suppressed by PJT. These results indicate that PJT exhibits anti-obesity effects in obese mice by decreasing in serum lipid levels and lipogenesis related gene.

This study was conducted to evaluate the growth conditions and vegetation of Peucedanum japonicum habitatin uninhabited islands of Incheon Korea. The emergence area of Peucedanum japonicum was 4.9m distance in coastline,and altitude and slope was 7.4m and 47.5%. Soil pH, organic matter and P2O5 of habitat were 7.1, 8.3% and 29.0㎎/㎏, andcontents of K, Ca, Mg and Na were 0.66, 4.9, 3.4 and 1.13cmol+/kg, respectively. Growth plants with Peucedanum japonicumwere total 55 taxa, and Gakeuldo in uninhabited islands was the most as 25 taxa. In growth plants with Peucedanumjaponicum, plants of over 25% frequence per plot were Miscanthus sinensis, Dendranthema boreale, Lilium lancifolium,Hemerocallis middendorffii, Elymus dahuricus, Cirsium japonicum var. maackii, Asparagus schoberioides, Gypsophila oldhamiana,Cocculus trilobus, Silene aprica var. oldhamiana, Artemisia capillaris, Crepidiastrum denticulatum and Sedum oryzifolium.The vegetation of Peucedanum japonicum habitat was classified into Aster spathulifolius community, Miscanthus sinensiscommunity, Dendranthema boreale community, Setaria viridis var. pachystachys community, Cirsium japonicum var. maackiicommunity and Hemerocallis middendorffii community. According to general condition of habitat, suitable growing areaswas determined to slightly acidic from mild alkaline soils.

This study was carried out in order to investigate the photosynthesis response and leaf characteristics of Peucedanum japonicum growing in forest farming. The experiment was performed by leaf mold (pine tree and chestnut tree) and shading levels (0%, 35%, 50% and 75% shading). Light relative intensity was 100% (full sunlight), 60.3% (35% shading), 35.1% (50% shading), and 17.4% (75% shading) respectively. Light response curves of pine-leaf mold and chestnut-leaf mold were the highest in control (full sunlight) and these were getting lower in the higher shading level. Photosynthesis capacity and light saturation point were indicated higher in chestnut-leaf mold within the same shading level. As the shading level increased, maximum photosynthesis rate decreased. And apparent quantum yield was not indicated statistically significant difference from all treatment. Leaf area, leaf length and leaf width were significant higher in 35% shading and control under chestnut-leaf mold in all treatment. As the shading level increased, LAR (leaf area ratio), SLA (specific leaf area) and SPAD value decreased in pine-leaf mold and chestnut-leaf mold. As a result of surveying the whole experiment, P. japonicum is judged better growth and higher yield by maintaining 35% shading (relative light intensity 60%) under chestnut-leaf mold in forest farming.

본 연구에서는 재배조건(하우스재배, 노지재배), 수확시기(어린잎, 성잎) 열처리에 따른 영양성분 및 항산화활성의 변화를 비교 분석하여 국가표준성분표 발간을 위한 기초자료로 활용하고자 하였다. 일반성분 4종(수분, 지질, 회분, 단백질), 무기질 5종(칼슘, 철, 마그네슘, 칼륨, 나트륨), 비타민 3종(비타민 C, 엽산, 베타카로틴)의 영양성분을 분석하였고 폴리페놀 화합물과 DPPH 라디칼 제거능을 각각 측정하였다. 연구결과 일반성분의 경우 시료 간에 유의적인 차이를 나타내지 않았으나 무기질의 경우 Ca, Mg, Na은 하우스 재배가 노지재배 보다 유의적으로 높은 함량을 나타내었다. 비타민함량과 폴리페놀 함량 및 항산화 활성의 경우 수확시기보다는 재배방법과 열처리에 의해 함량변화가 큰 것으로 나타났다. 하우스보다 노지 재배된 갯기름나물의 비타민 C, 엽산, 폴리페놀 화합물과 항산화활성이 증가하였으며 열처리할 경우 비타민 C, 베타카로틴, 폴리페놀 화합물, 항산화활성이 각각 증가하였다. 비타민 C의 기존 보고된 이론과 상이한 결과가 도출되어 다양한 열처리온도와 시간을 적용한 성분 변화 연구가 필요할 것으로 생각된다. 베타카로틴과 폴리페놀 화합물의 열처리에 의한 증가는 산화가수분해 효소의 불활성화와 세포조직의 견고성 저하로 인한 활성물질의 추출 효율증가로 설명될 수 있다. 따라서 나물을 데쳐서 섭취할 경우 미생물 오염방지 등 안전성을 확보할 수 있을 뿐만 아니라 나물 세포조직이 연해져 베타카로틴과 같은 우리 몸에 유익한 성분의 소화흡수율이 증가될 것으로 생각된다.

This study was carried out in order to investigate the early growth and root development characteristics of Peucedanum japonicum container seedlings. The experiment was performed by containers (128 and 200 cavities containers) and shading levels (0%, 35%, 50% and 75% shading). Germination rate of P. japonicum seeds was getting higher in the lower shading level and the highest in the full sunlight (71.9%). Plant height was the highest under 50% shading of all containers. Fresh weight and dry weight of the shoot (leaves + stem) were the highest under 50% shading of all containers, too. Meanwhile, fresh weight and dry weight of the root per plant were the highest under the full sunlight of 128 cavities container such as 0.34 g and 0.03 g, respectively. Total root length, root project area and root surface area were higher under the full sunlight of 128 cavities container such as 234.5 cm, 6.6cm2 and 20.8cm2, respectively and the next higher was under 35% shading such as 201.7 cm, 5.9cm2 and 18.4cm2, respectively. A case of root volume was the highest with 0.15cm3 under the full sunlight. As a result of the surveying the whole experiment, it is concluded that the shoot and root of P. japonicum seedling grow nicely by maintaining 35% shading.

This study was conducted to investigate the changes in the physicochemical properties of Peucedanum japonicum Thunb. after blanching. After blanching treatment, the crude protein content decreased but did not affect the crude fat, crude ash and carbohydrate contents of P. japonicum. All the detected free sugar contents decreased after blanching, but the total free sugar content decreased by 14.8% from that of the raw P. japonicum after blanching. The total amino acid and essential amino acid contents of the blanched P. japonicum. decreased by 10.75% and 15.22% from those of the raw P. japonicum. There were no differences in the contents of the total fatty acid between the raw and blanched P. japonicum. The total organic acid content decreased by 37.03% from that of the raw P. japonicum. after the blanching, and the reduction of the acetic acid was largest in the organic acid. The vitamin A, C and E contents decreased by 20.20%, 8.23% and 35.59% after the blanching. The total mineral content of the blanched P. japonicum. decreased by 21.84% after the blanching. The nutrients in the P. japonicum were essential amino acid, vitamin C, minerals, but these were reduced after the blanching. Therefore, the blanching conditions that can reduce nutrient loss of P. japonicum must be established.

This study was conducted to evaluate the vegetation structure of the Peucedanum japonicum community by the phytosociological method of floristic composition table and cluster analysis on the southern coast of Korea. The vegetation of the Peucedanum japonicum community was classified mainly into 2 communities such as the Miscanthus sinensis community and the Lysimachia mauritiana-Rosa wichuraiana community. The Carex boottiana and Sedum oryzifolium community were classified as the lower rank of Miscanthus sinensis community. On level 1 of the cluster analysis of plant species, they were classified into Lysimachia mauritiana and Rosa wichuraiana group, also Miscanthus sinensis, Carex boottiana and Sedum oryzifolium in Peucedanum japonicum community, which is similar to the community classification shown in the synoptic table. On level 1 of the cluster analysis of relev, inland coast with Jejudo was Lysimachia mauritiana and Rosa wichuraiana of group such as level 1 of the cluster analysis of plant species, and island coast with Geomundo was Miscanthus sinensis Carex boottiana and Sedum oryzifolium of group such as cluster analysis of plant species.

This study was conducted to evaluate the vegetation structure and ordination of the Peucedanum japonicum Thunb. community by the Phytosociological method, floristic composition table on east coast of South Korea. The vegetation of Peucedanum japonicum Thunb. community was classified into 2 communities such as the Aster Spathulifolius community and the calystegia soldanella-Artemisia princeps community. Vegetation of the Peucedanum japonicum Thunb. community with the Aster spathulifolius community was shown southward, whereas vegetation of Peucedanum japonicum Thunb. community with the calystegia soldanella-Artemisia princeps community was shown northward from the base line of Pohang at North latitude of 36" 05'. Accordingly, the Peucedanum japonicum Thunb. community was grown commonly with the Aster spathulifolius community southward of the base line of Pohang, whereas it was grown commonly with the calystegia soldanella-Artemisia princeps community northward on the east coast. All environmental conditions of habitat taken together, the optimum habitat of Peucedanum japonicum Thunb. was at Ulsan geographically and the middle region of sea cliff topography, and was suitable for alkali. sandyloam.

Five coumarins, psoralen (1), scopoletin (2), isoimperatorin (4), (+)-marmesin (5) and xanthotoxin (6), three chromones, cimifugin (3), hamaudol (7) and sec-O-glucosylhamaudol (10), one sterol, daucosterol (8) and one aliphatic alcohol, galactitol (9) were isolated from the root of Peucedanum japonicum. Their chemical structures were identified by the physicochemical and spectroscopic data by comparing literature values. Among them, compounds 9 and 10 were isolated for the first time from this plant. The anti-inflammatory effects of isolated compounds were examined on cyclooxygenase (COX), compounds 1, 2 and 7 showed inhibitory activity on COX-1 with IC50 values of 0.88, 0.27 and 0.30 mM, respectively. In the test for COX-2 activity, only compound 7 showed significant inhibitory activity with the IC50 value of 0.57 mM. The other compounds exhibited weak inhibitory or no inhibitory activity.

The objective of this study is to investigate morphological characteristics of seeds and effects of environmental factors such as light and temperature on the germination and seedling growth of Peucedanum japonicum Thunberct, Which belongs to Umbelliferae and useful for on oriental medicine materials. Seed shape of Peucedanum japonicum Thunberct is ovate. Color of seed coats varied from yellow to dark brown. Seed size is 5.3mm in length, 4.2mm in width. One thousand-seed weight of Peucedanum japonicum Thunberct is 4,010mg. Optimum temperatures for seed germination and seedling growth range from 20 to 25℃.

한약재로 이용되고 있는 육묘한 식방풍을 공시하여 육묘이식 백색비닐피복, 육묘이식 흑색비닐피복, 육표이식 무피복 처리와 노지직파 무피복처리가 생육과 수량에 미치는 영향에 관하여 시험을 수행한 결과는 다음과 같다. 출아기간은 폿트육묘시 8일이었고 노지직파는 40일이었으며 출아율은 폿트육묘시 74%였으나 노지직파에서는 17%로 크게 저조하였다. 초장, 엽수, 경직경 등 지상부생육량은 육묘이식 백색비닐피복〉육묘이식 흑색비닐피복 〉육묘이식 무피복〉노지직파 무피복처리구 순으로 많은 경향이었다. 주근의 길이는 육묘이식 무피복구가 유의하게 짧았으나, 피복처리구와 노지직파 무피복구는 비슷하게 긴편이었다. 주근의 굵기는 비닐피복구가 무피복구에 비하여 유의하게 굵었고 노지직파 무피복구가 가장 가늘었다. 지근의 수는 육묘이식한 것이 직파한 것보다 4.7~7.1개 많았다 건근비율은 노지직파 무피복구에서 30.5%로 가장 높고 육묘이식구에서는 무피복구가 높았으머 비닐피복구에서 낮은 경향이었다. 10a당 건근수량은 육묘이식 백색비닐피복 542kg, 육묘이식 흑색비닐피복 506kg, 육묘이식 무피복 419kg으로 노지직파 7kg에 비하여 각각 52, 42, 17%증가하였으며 식방풍의 재배는 육묘이식하되 백색비닐로 피복하여 재배하는 것이 가장 유리하였다.

1998년 6~9월의 강수량이 1,042mm, 강수일수가 60일이나 되어 배수조건이 나쁜 포장의 밭작물에 습해가 심하게 발생되었던 안동지역에서 습해정도에 따른 식방풍과 지황의 생육특성 및 수량을 농가포장에서 조사하였다. 식방풍은 습해가 심할수륵 지상부보다 지하부의 생육장해가 더 심하였으며, 주근의 신장생장이 비대생장보다 현저하게 억제되었다. 습해 정도별로 ha당 생근수량은 정상생육에 비하여 21~80% 감소되었다. 지황은 습해 정도별로 고사주율이 52%였으며, 습해에 의하여 근경의 형성 및 발육이 장해를 받아 습해가 심할수록 주당 근경수와 근경의 직경이 크게 감소되었다. 따라서 습해가 심할수록 상품성이 높은 근경의 수량이 현저하게 감소되었으며, 습해 정도별로 ha당 근경의 생체수량은 정상생육에 비하여 19~83%감소되었다.

식방풍 1호는 1990년에서 1995년까지 6개년간 전국에서 수집한 11개 식방풍재래종 중 영덕종 등 4계통을 순계분리한 후 생산력 검정시험에 공시한 다음 그 중 생육특성이 우수한 영덕종과 봉화종에 대해 지역적응시험 및 농가실증시험을 실시한 결과 영덕종이 다수성 순계임이 확인되어 1995년도에 농촌진흥청 우량종자선정 심의회에서 우량품종으로 결정되어 식방풍 1호로 명명되었기에 육성 과정에서 나타난 주유특성과 수량성을 요약하면 다음과 같다. 1. 식방풍 1호는 봉화식방풍과 초장은 비슷하고 주근장은 짧았으나 엽수가 많고 경자과 근두직경은 굵었고 종실량도 증수되었다. 2. 식방풍 1호는 근피색이 황갈색이고 근 형태는 식립원추형으로서 양질의 생약 재 생산에 유리한 품종이었다. 3. 식방풍 1호는 근중 엑스함량과 회분 및 산불용성 회분 함량으로 보아 대한 약전 규격집에 규정된 함량치에 적합하여 생약으로 이용시 유효성을 갖추었다. 4. 식방풍 1호는 토양선층에 비교적 강한 편이었고 내병성, 내습성, 내서성 및 내도복성도 강한 특성을 나타내었다. 5. 식방풍 1호의 건근수량은 생산력 검정시험, 지역적응시험 및 농가실증시험 결과 모두 봉화 식방풍보다 ha당 10~28% 증수되었다.

본 연구는 1991년부터 1992년까지 2년간 경상북도 농촌진흥원 특용작물 시험포장에서 봉화 지방종의 식방풍을 공시하여 무피복 파종시기 및 피복 재료와 흑색비닐피복 파종기, 재식밀도를 구명키 위해 시험한 결과를 요약하면 다음과 같다. 1. 무피복 파종기는 관행 3월 30일 파종보다 3월 20일 파종이 지상부 및 지하부 생육이 양호하였고 건근수량이 8%나 증수되었다. 2. 흑색비닐피복이 무피복에 비해 출현기간이 단축되고 주근장, 근직경, 건근비율이 높아 건근 수량이 10%나 증수되었다. 3. 흑색비닐 파종적기는 관행 3월 30일의 10a당 건근수량이 548kg인데 비해 3월 20일 파종에서 6% 증수되었다. 4. 식방풍의 재식밀도는 관행 30×20cm의 10a당 건근수량이 388kg인데 비해 20×15cm가 50% 증수되었으며 10a당 재식주수는 3.333주이었다.