The objective of this study was to investigate the effects of combinations of tofu paste and non-starch polysaccharides (NSP) on the oil uptake reduction (OTR) of deep-fat fried cake doughnuts. OTR agents were tofu paste (from grinding tofu with deionized water, followed by passage through a 60 mesh sieve), and five neutral and nine anionic NSPs. A control doughnut (without tofu paste or NSP), tofu doughnut (with tofu paste) and NSP-tofu doughnut (with tofu paste and NSP) were prepared. The moisture and total lipid (TL) content, cross-section image, color characteristic, and specific volume were measured. The tofu and NSP-tofu doughnuts exhibited higher moisture and lower TL content than the control. OTR was 10.8% for the tofu doughnut, and between 13.2% and 41.2% for the NSP-tofu doughnut. The highest OTR (41.2%) was found in the NSP-tofu doughnut with a combination of tofu paste and sodium alginate (NaA). The specific volume of the NSP-tofu doughnuts with combinations of tofu paste with NaA (2.5 mL/g), locust bean gum (2.5 mL/g), and κ-carrageenan (2.4 mL/g) was very close to that of the control (2.6 mL/g). Considering the OTR and specific volume of doughnuts, the combination of tofu paste and NaA would be most effective in reducing the oil uptake of doughnuts during deep-fat frying.

This study investigated the effect of high concentration of free ammonia on microalgal growth and substrate removal by applying real wastewater nitrogen ratio. To test of this, the conditions of free ammonia 1, 3, 6, 9, 12, 15 mg-N/L are compared. After 3 days of incubation, algal growth of Chlorella vulgaris and carbon removal rate are respectively lower in the reactors of FA 12, 15 mg-N/L compared to the others. This indicates that the high concentration of free ammonia, in this case, above 12 mg-N/L, has negative effect on algal growth and metabolic activity. Also, high concentration of free ammonia causes the proton imbalance, ammonium accumulation in algae and has toxicity for these reasons. So, we have to consider free ammonia in applying the microalgae to wastewater treatment system by the way of diluting wastewater or controlling pH and temperature.

The objective of the research was to identify the presence of adiponectin receptors and to study adiponectin action on glucose uptake and growth in mouse mammary epithelial cells. These cells expressed adiponectin receptors, AdipoR1 and AdipoR2. Insulin (10 ng/ml) or insulin like growth factor-I (IGF-I, 10 ng/ml) alone did not alter the degree of AdipoR1 and AdipoR2 genes expression from 0 to 4 h incubation. Prolactin (10 ng/ml) or epidermal growth factor (EGF, 10 ng/ml) alone also did not induce the two genes’ mRNA in the incubation time. Adiponectin (1 μg/ml) alone or pre-incubation of insulin alone (100 ng/ml) for 2 h prior to adiponectin stimulation did not increase 2-deoxy-D-glucose,[1,2-3H] uptake but adiponectin+pre-incubation of insulin significantly increased glucose uptake compare to control (p<0.05). In a similar way, insulin alone or pre-incubation of adiponectin alone (2 h) did not increase glucose uptake but insulin+pre-incubation of adiponectin increased glucose uptake compare to control (p<0.05). Insulin sensitization for 2 h prior to adiponectin stimulation tended to increase glucose uptake response by the following adiponectin stimulation showing small interaction effect between insulin and adiponectin (p<0.1). However, adiponectin sensitization for 2 hours prior to insulin stimulation did not shown interaction effect between adiponectin and insulin (p>0.1). The glucose uptake by both of hormones seems to be not interactive but additive (p<0.05). Adiponectin in the presence of 2% FBS decreased DNA synthesis of mammary epithelia (p<0.05). AICAR (100 or 200 μM), AMPK activator, decreased mammary epithelial cell growth in the presence of 2% FBS. These results indicate that adiponectin pathway has inhibitory effect on mammary epithelial cell growth.

Task-based language instruction has been increasingly promoted in second language (L2) classrooms under the assumption that it can provide an ultimate condition for L2 learning when combined with form-focused interventions such as teacher corrective feedback (CF). The present research investigated the amount, type, and learner uptake of teacher CF in relation to the following task-related variables: (1) task vs. nontask work, (2) focused vs. unfocused tasks, and (3) open vs. closed tasks. Data were collected from 8 intermediate adult EFL classrooms. It was found that the amount of teacher CF provided during tasks versus nontasks and focused versus unfocused tasks was not significantly different. However, the teachers used different strategies depending on the focus of tasks. The study did not relate the matter of open versus closed tasks to the amount and types of teacher CF. When considering learner uptake, the study found that the teachers tended to offer more uptake opportunities during relatively form-oriented activities, and the learners’ uptake was more successful when they were working in relatively form-oriented activities compared to the cases where they were in meaning-based activities. Learner uptake was not associated with whether or not tasks were open and closed.

In order to understand the contribution of seaweed aquaculture to nutrients and carbon cycles in coastal environments, we measured the nutrients & carbon uptake rates of Porphyra yezoensis Ueda sampled at Nakdong-River Estuary using a chamber incubation method from November 2011 to April 2012. It was observed that the production rate of dissolved oxygen by P. yezoensis (n=30~40) was about 68.8±46.0 μmol gFW -1 h-1 and uptake rate of nitrate, phosphate and dissolved inorganic carbon (DIC) was found to be 2.5±1.8 μmol gFW -1 h-1, 0.18±0.11 μmol gFW -1 h-1 and 87.1±57.3 μmol gFW -1 h-1, respectively. There was a positive linear correlation existed between the production rate of dissolved oxygen and the consumption rates of nitrate, phosphate and DIC, respectively, suggesting that these factors may serve as good indicators of P. yezoensis photosynthesis. Further, there was a negative logarithmic relationship between fresh weight of thallus and uptake rates of nutrients and CO2, which suggested that younger specimens (0.1~0.3 g) were much more efficient at nutrients and CO2 uptake than old specimens. It means that the early culturing stage than harvesting season might have more possibilities to be developed chlorosis by high rates of nitrogen uptake. However, N & C demanding rates of Busan and Jeollabuk-do, calculated by monthly mass production and culturing area, were much higher than those of Jeollanam-do, the highest harvesting area in Korea. Chlorosis events at Jeollabuk-do recently might have developed by the reason that heavily culture in narrow area and insufficient nutrients in maximum yield season (Dec.~Jan.) due mostly to shortage of land discharge and weak water circulation. The annual DIC uptake by P. yezoensis in Nakdong-River Estuary was estimated about 5.6×103 CO2 ton, which was about 0.03% of annual carbon dioxide emission of Busan City. Taken together, we suggest more research would be helpful to gain deep insight to evaluate the roles of seaweed aquacul-ture to the coastal nutrients cycles and global carbon cycle.

본 연구에서는 천안시 두정공원을 대상으로 도시공원의 식재유형별 수목의 탄소저장량, 연간 탄소흡수량, 연간 토양호흡량을 측정하여 비교하였다. 두정공원에서 소나무식재림, 상수리나무군락, 상수리나무-아까시나무식재림, 아까시나무식재림을 선정하여 2012년 3월부터 2013년 2월까지 각 식재림을 분석하였다. 탄소저장량과 연간 탄소흡수량은 수목의 흉고직경을 이용한 상대생장식을 활용하여 산정하였고 토양호흡량은 Li-6400을 이용하여 측정하였다. 소나무식재림, 상수리나무군락, 상수리나무-아까시나무식재림, 아까시나무식재림에서의 탄소저장량은 각각 17.36, 88.63, 115.38, 49.88 ton C ㏊⁻¹ 였고, 연간 탄소흡수량은 각각 1.04, 2.12, 6.47, 3.67 ton C ㏊⁻¹ yr⁻¹로 산정되었다. 개체목당 평균 연간 탄소흡수량은 소나무, 상수리나무, 아까시나무에서 각각 1.81, 17.86, 9.14 ㎏ C·tree yr⁻¹로 상수리나무가 가장 높았다. 평균 토양호흡량은 각 식재유형별로 2.20, 1.90, 2.47, 2.51 μ㏖ CO2 m⁻² s⁻¹로 측정되었고 연간 토양호흡량은 각각 6.66, 5.33, 7.20, 7.25 ton C ㏊⁻¹ yr⁻¹로 추정되었다. 본 조사지의 식재유형 중 상수리나무-아까시나무식재림이 탄소저장량과 연간 탄소흡수량이 가장 많아 공원의 탄소흡수원 역할에 크게 기여했고, 소나무식재림은 가장 적게 평가되었다. 본 연구결과는 이산화탄소 흡수원의 역할을 하는 도시공원 수목의 식재와 관리에 필요한 자료로 활용될 수 있다.

Nanotechnology has become one of the fastest developing technologies and recently applied to a variety of industries. Thus, increasing number of nano materials including various nanoparticles would be discharged into wastewater and consequently entering a biological wastewater treatment process. However, the impact of the nano particles on biological wastewater treatment has not been estimated intensively. In this research, we investigated the effect of silica nanoparticle on the oxygen uptake rates (OURs) of activated sludge used in a conventional wastewater treatment process. The inhibition (%) values were estimated from the results of OURs experiments for the silica nanoparticles with various sizes of 10-15, 45-50, and 70-100 nm and concentrations of 50, 250, and 500 ppm. As results, the inhibition value was increased as the size of silica nano particles decreased and the injected concentration increased. The maximum inhibition value was investigated as 37.4 % for the silica nanoparticles with the size of 45-50 nm and concentration of 50 ppm. Additionally, the effect of size and concentration on the inhibition should be considered cautiously in case that the aggregation of particles occurred seriously so that the size of individual particles was increased in aquatic solution.

As a replication study of Hanaoka and Izumi (2012), the present research investigated the process of learners’ noticing of problems in their interlanguage (IL) while they were producing the second language (L2) and their process of relevant input provided in the form of two types of feedback (i.e., reformulation and a model text) in L2 writing. The data were collected from twenty-five university students in Korea, and they engaged in a four-staged picture description task consisting of the writing stage (Stage 1), the comparison stage (Stage 2), the immediate revision stage (Stage 3), and the delayed revision stage (Stage 4). It was found that output triggered learners’ noticing of problems; however, the problems did not always show up overtly in their writing. Some problems were manifested in writing (i.e., overt problems), while some were hidden (i.e., covert problems). A model text and reformulation played somewhat different roles in offering the solutions to the problems: a model text provided more solutions to covert problems while reformulation provided more solutions to overt problems. And, the extent of learners’ noticing was associated with the types of problems (overt vs. covert problems) and feedback (i.e., models vs. reformulation). However, the types of problems and feedback were not related to the rate of incorporation of feedback into subsequent writing.

본 연구는 짚신나물 열수 추출물의 α-glucosidase 저해 활 성을 측정하고, 분화된 근육세포에서 glucose 이용과 인슐린 신호전달에 미치는 영향을 분석하였다. 짚신나물 열수 추출 물(10 ㎎/㎖)은 α-glucosidase 활성을 67% 저해하였으며, 같은 농도의 양성대조구인 acarbose(63%)와 유사한 저해 효과를 보였다. 짚신나물 열수 추출물이 α-glucosidase에 의한 단당 류 생성을 저해함으로 식사 후 혈당이 급격히 상승하는 것을 억제하는데 효과적인 소재로 이용 가능성을 확인하였다. 또한 근육세포에서 인슐린 저항성을 유발하기 위해 지방산(1 mM, palmitic acid)를 처리하였고, glucose의 세포내 유입이 감소 되는 것을 확인하였다. 지방산 처리 세포 모델에서 짚신나 물 열수 추출물(10 ㎍/㎖)은 glucose 이용을 유의적으로 회복 시켜 주었다. Normal 상태의 배양조건에서 근육세포의 포도 당 이용능은 짚신나물 열수 추출물(100 ㎍/㎖) 처리에 의해 유의적으로 증가하였다. 근육세포 내로 glucose 유입은 운반 단백질인 Glut4를 통해 이루어지며, 이것은 인슐린이 신호전 달을 통해 조절한다. 짚신나물 열수 추출물의 세포 내 glucose 이용 증가 효과는 인슐린 신호전달 관련 분자인 Akt 유전자 와 단백질 발현을 증가시킨 것과 관련되는 것으로 추정된다. 결론적으로, 짚신나물 열수 추출물은 소화기관에서의 탄수화 물 흡수 저해와 근육세포 내 glucose 이용 증가를 통해 혈당 조절 및 당 대사 개선에 긍정적인 영향을 미치고 있음을 확인 하였다.

본 연구는 도시의 주요 상록 조경수종인 소나무와 잣나무를 대상으로, 직접수확법을 통해 탄소저장 및 흡수를 용이하게 추정하는 회귀모델을 제시하고 도시수목의 탄소저감 효과를 계량화하는데 필요한 기반정보를 구축하였다. 수종별로 유목에서 성목에 이르는 일정 간격의 흉고직경 크기를 고려하여 개방 생장하는 조경수목을 구입한 후, 근굴취를 포함하는 직접수확법에 의해 개체당 부위별 및 전체 생체량을 측정하고 탄소저장량을 산출하였다. 또한, 흉고 부위의 수간원판을 채취하여 직경생장을 분석하고 탄소흡수량을 산정하였다. 흉고직경을 독립변수로 생장에 따른 수종별 단목의 탄소저장 및 흡수를 계량화하는 활용 용이한 회귀모델을 유도하였다. 이들 회귀식의 r2는 0.98 이상으로서 적합도가 상당히 높았다. 동일 직경의 탄소저장 및 흡수량은 유목의 경우 소나무가 잣나무보다 더 많았으나, 20cm 이상 성목의 경우 생장량 차이에 기인하여 그 반대인 경향을 보였다. 흉고직경 25cm인 소나무와 잣나무는 각각 115.6kg, 130.0kg의 탄소를 저장하고, 연간 9.4kg, 14.6kg의 탄소를 흡수하는 것으로 나타났다. 본 연구는 조경수목의 직접 벌목 및 근굴취의 난이성에 기인하여, 생체량 확장계수, 지하부/지상부 비율, 직경생장 등 산림수목의 계수를 대용하여 대상수종의 탄소저감을 계량화한 기존 연구의 한계성을 극복할 새로운 초석을 마련하였다.

본 연구는 도시의 주요 상록 조경수종인 소나무와 잣나무를 대상으로, 직접수확법을 통해 탄소저장 및 흡수를 용이하게 추정하는 회귀모델을 제시하고 도시수목의 탄소저감 효과를 계량화하는데 필요한 기반정보를 구축하였다. 수종별로 유목에서 성목에 이르는 일정 간격의 흉고직경 크기를 고려하여 개방 생장하는 조경수목을 구입한 후, 근굴취를 포함하는 직접수확법에 의해 개체당 부위별 및 전체 생체량을 측정하고 탄소저장량을 산출하였다. 또한, 흉고 부위의 수간원판을 채취하여 직경생장을 분석하고 탄소흡수량을 산정하였다. 흉고직경을 독립변수로 생장에 따른 수종별 단목의 탄소저장 및 흡수를 계량화하는 활용 용이한 회귀모델을 유도하였다. 이들 회귀식의 r2는 0.98 이상으로서 적합도가 상당히 높았다. 동일 직경의 탄소저장 및 흡수량은 유목의 경우 소나무가 잣나무보다 더 많았으나, 20㎝ 이상 성목의 경우 생장량 차이에 기인하여 그 반대인 경향을 보였다. 흉고직경 25㎝인 소나무와 잣나무는 각각 115.6㎏, 130.0㎏의 탄소를 저장하고, 연간 9.4㎏, 14.6㎏의 탄소를 흡수하는 것으로 나타났다. 본 연구는 조경수목의 직접 벌목 및 근굴취의난이성에 기인하여, 생체량 확장계수, 지하부/지상부 비율, 직경생장 등 산림수목의 계수를 대용하여 대상수종의 탄소저감을 계량화한 기존 연구의 한계성을 극복할 새로운 초석을 마련하였다.

Despite a growing interest in the role of written corrective feedback (CF) in L2development, not enough research has examined how learners actually process and understand linguistic information delivered in CF. Acknowledging the importance and necessity of process-oriented research into written CF, the present study investigated the extent to which Korean learners of English understood written CF they received from the teacher in writing classes. In addition, the study looked into whether the presence and quality of understanding of CF was related to learners’immediate uptake and retention of CF. Two intact writing classes and one native English teacher participated in the present research. The study found that a third of CF targeting grammar errors and a half of CF targeting vocabulary errors were correctly understood. The quality of the learners’ understanding of CF was closely related to their immediate uptake in terms of grammar but not in vocabulary; and the quality of their understanding was strongly related to retention in grammar and vocabulary.

In this study, we observed anti-diabetic effects of acid hydrolyzed silk peptides, where the amount of peptides in the total amino acid mixture was strictly regulated. Using in vitro diabetes models, silk peptide-containing amino acid mixtures of 5.60% (G5), 11.30% (G10), 14.50% (G15), and 20.50% (G20) were examined separately in order to determine whether they have biological activities. According to our results, a cytoprotective effect was observed following treatment of interleukin-1β in RINm5f pancreas β-cells. As a consequence, Bax, a pro-apoptotic gene, was down-regulated, while Bcl-2, a pro-survival gene, was retained at normal level. Results of the 4’,6-diamidino-2-phentylindole (DAPI) staining assay confirmed that G20 has a better cytoprotective effect. Insulin release from RINm5f cells showed a significant increase following treatment with G5-G20, suggesting that silk peptide effectively regulated and induced insulin production. Single treatment with G5-G20 resulted in enhanced glucose uptake in L6 skeletal muscle cells. In addition, a higher amount of each group inhibited the activity of α-glucosidase. In summary, these data suggest that silk peptide may have an anti-diabetic effect through protection of pancreas β-cells and enhancement of insulin release, which showed a close association with Type 1 diabetes mellitus (DM), and can improve glucose uptake, which was the major target for therapy of Type 2 diabetes. Taken together, we concluded that acid hydrolyzed silk peptides can be used effectively for control of blood sugar metabolism via improvement of the problematic indices of Type 1 and Type 2 DM.

본 연구는 탄소 : 질소(탄질)비가 다른 유기자재를 시용하였을 때 시기별 양분의 동태와 유기재배 수수*수단그라스의 비료이용율에 미치는 영향을 조사하였다. 처리구는 질소함량 210kg/ha 수준으로 축분퇴비, 알팔파, 유박, 화학비료를 포함하였다. 유박과 알팔파는 가장 낮은 탄질비를 나타내었고 축분퇴비는 33.7 : 1의 가장 높은 탄질비를 보였다. 축분퇴비는 시용량이 가장 많았고 이에 따라 토양내 양분농도를 증가시켰다. 탄질비가 낮은 유박과 알팔파는 축분퇴비에 비해서 질소의 무기화율을 촉진시켰다. 질소이용효율은 화학비료구에 비해서 알팔파와 유박이 약 60% 전후를 나타내었고, 축분퇴비는 20% 이하의 효율이 관찰되었다. 인산의 이용효율은 알팔파가 가장 높았고 축분퇴비가 가장 낮았다. 이에 따라 탄질비를 고려한 유기자재를 선택해서 밭토양에 시비를 해야 할 것으로 판단되었다.

가뭄 스트레스 조건에서 마이코라이자의 접종이 질소의 흡수와 동화에 미치는 영향을 구명하기 위해 마이코라이자를 접종한 처리구와 접종하지 않은 처리구에서 수분처리 7일간 총 , 흡수된 로부터 아미노산과 단백질로 합성된 15N 함량을 각각 분석하였다. 정상 관수구에서는 전 조사항목에서 마이코라이자 접종에 대한 유의적인 효과가 나타나지 않았다. 총 함량은 가뭄 스트레스에 의해 마이코라이자 접종구 및 비접종구에서 각각 13.8%, 28.5% 감소하였다. 함량은

Learner immediate response (i.e., uptake) to recasts has been discussed in a considerable amount of research in the field of second language acquisition (SLA). Many different interpretations of learner uptake have been proposed. One of them defines uptake as an indication of learner noticing of recasts. However, only a limited amount of research has actually examined how learner uptake is associated with learner perception of recasts. The current study examined how learners actually respond to recasts provided in the classroom. In addition, it investigated whether or not learner uptake was related to learner perception of recasts and how the quality of learner uptake was associated with the depth of learner awareness. Nine intact English classrooms were observed and video-taped. Eighty-eight students participated in either oral or written stimulated recall sessions. They viewed the video clips of their own classes and recalled the moments when they received recasts. The analyses of classroom interaction and students’ comments indicated that they responded to recasts to a considerable extent when they had an opportunity for uptake. Learner uptake of recasts was found to be related to learner perception of recasts. It was also revealed that the students much more frequently perceived the gap when they showed successful uptake compared to the cases where they did not.

유채 (Brassica napus L.)에서 황 공급수준에 따른 글루타치온 함량의 변화가 황 흡수 및 동화관련 효소 활력에 미치는 영향을 규명하고자, 농도를 4수준 (0, 0.1, 1.0 및 2.0 mM)으로 25시간 처리 한 후 식물조직 내 글루타치온 함량을 측정하고, 흡수, ATP sulfurylase (ATPS) 및 O-acteylserine (thiol) lyase (OASTL) 효소 활력과의 상관관계를 분석하였다. 흡수는 황 공급수준에 따라 평

본 연구는 저토심 옥상녹화에 적용 가능한 초본류 및 지피식물을 중심으로 식물의 광합성작용을 통한 CO2 흡수량과 증발산량을 정량화하여 도시미기후 관점에서의 옥상녹화식물의 환경성능을 평가하고자 하였다. 이를 위해 옥상녹화용 초본류 7종을 대상으로 적외선 CO2 가스 분석기에 의한 CO2 교환 속도 분석을 통해 각 식물의 CO2 흡수량과 증발산량을 측정하였다. 실험기간은 생장이 활발해지기 시작하는 5월부터 11월까지 매월 2반복 측정하였고, 주변 환경에 예민한 초본류의 특성상, 환경변수가 고정된 실내에서 광도의 변화를 주어 실시하였다. CO2 흡수량과 증발산량을 산출한 결과 단위엽면적당 CO2 흡수량은 초본류 중 구절초가 21.47×10-6g/cm2/s, 매발톱꽃이 12.74 g×10-6g/cm2/s로 높은 흡수율을 보였고, 켄터키블루그래스도 16.20×10-6g/cm2/s로 비교적 높았다. 단위엽면적당 증발산량은 켄터키블루그래스가8.75×10-5g/cm2/s로 가장 많았고, 다음은 매발톱꽃 8.66×10-5g/cm2/s, 구절초 8.58×10-5g/cm2/s 순으로 나타났다.

Ganoderma lucidum has long been used as a potent medicinal plant. In addition, recent studies showed that G. lucidum is used to prevent or treat various human diseases such as allergy, hepatopathy, hypertension, cancer and diabetes. In this study showed that ethanol extract from G. lucidum have potential inhibitory effects of glycerol 3-phosphat acyltransferase (GPAT) activity and increase of glucose uptake on skeletal muscle cells. Inhibition of GPAT, which catalyze the first step in de novo TAG synthesis, has been proposed as one of the drug targets for insulin resistance and type-2 diabetes. G. lucidum extract inhibited GPAT activity. Bioassay-guided fractionation and isolation of bio-active substances from ethanol extracts of G. lucidum were carried out by using chromatographic techniques and in vitro GPAT enzyme assay. One of bioactive molecules, ergosterol peroxide, was isolated and identified by physical and spectral properties. In this study showed that ethanol extract from G. lucidum and ergosterol peroxide have potential anti- diabetes effects through the increase of glucose uptake. This was as sociated with increased activity AMP-activated protein kinase(AMPK). AMPK is another regulatory protein in the glucose uptake pathway and energy metabolism. The result was that the increase of glucose uptake by G. lucidum extract might be mediated by AMPK.