델파이 방법을 사용한 본 연구는 북한의 축산개발을 위한 미래 지원기술을 예측하고 도출하도록 설계되었다. 지원기술은 북한의 축산을 미래의 지원 프로젝트에서 높은 가치를 지닌 핵심전략으로 추진되어 질 것이다. 이러한 맥락에서 설문지를 각 기술 전문가에게 배포하여 각 지원 항목의 중요성과 실현시기를 조사하였다. 이 설문 조사는 또한 남한과 비교했을 때 북한의 이러한 항목들 각각에 대해 연구 개발 수준과 자원분배를 조사하는 것을 목표로 했다. 델파이의 조사는 북한의 축산지원 전문가들에게만 국한되었다. 총 10가지 핵심기술문제가 공식화되었고, 25명의 전문가가 질문에 응답했다. 첫 번째 실현단계는 5년 이후에 26개 항목 중 절반이 47.8%를 기록했으며, 두 번째 실현연도는 평균 6.7년 이었다. 따라서, 장기적인 전략 계획을 수립하고 실행해야 할 필요가 있다. 현재 북한의 가축생산량은 한국에 비해 49% 수준으로 추정되며, 지원 사업이 시급히 필요하다. 정책 수단의 지원 방법은 전문가 인력충원, 인프라 구축 및 자재지원을 합한 총 67.5%를 차지하면서, 이 세 가지 항목에 중점을 두어야한다. 평균 총 사업비는 126.6억 원이었고 전문가는 연간 16.8명, 평균 소요기간은 6.1년으로 각각 추정되었다.

본 연구는 퇴비단 내에서 소리쟁이 종자와 피 종자의 발아력 억제효과를 분석하기 위하여 염소 분 퇴비, 젖소 분 퇴비 그리고 한우 분 퇴비를 대상으로 하여 실험을 수행하였다. 소리쟁이 종자와 피 종자를 거즈에 싸서 퇴비단 내에 묻어놓고 1.5일, 3일, 10일, 20일 경과 후 종자를 회수한 뒤 발아력을 측정하였다. 실험 결과 소리쟁이 종자와 피 종자의 발아율은 가축 분의 종류와 퇴비화 방법에 관계없이 퇴비단의 온도와 직접적인 상관관계를 가지는 것으로 나타났다. 온도가 60∼70°C인 조건의 퇴비단에서 1.5일 동안 머무른 소리쟁이 종자와 피 종자는 발아력을 완전히 상실하였다. 반면에 퇴비단 최고 온도가 51°C 이하인 퇴비단에서는 소리쟁이 종자와 피 종자가 퇴비단 내에서 20일간 머무른 후에도 10 % 정도의 발아력을 유지하였다. 이상의 결과를 종합해보면, 소리쟁이 종자와 피 종자의 발아율을 억제하기 위해서는 가축 분 퇴비화단계에서 최소 55°C 이상의 온도를 3일 이상 유지하는 것이 좋다. 또한 퇴비단 온도가 60∼70°C 정도 상승한다면 약 1.5일 정도 체류하는 것으로도 소리쟁이 종자와 피 종자의 발아력을 억제할 수 있을 것이다. 퇴비화 방법을 기준으로 보면, 호기적 퇴비화 방법이 퇴비단의 온도상승기간이 더 짧았다. 따라서 호기적 퇴비화방법을 적용하는 것이 소리쟁이 종자와 피 종자의 발아력을 억제하는데 더 효율적일 것이다.

본 연구는 축산분뇨를 퇴비화 하는 과정에서 흔히 사용되는 수분조절제인 톱밥, 왕겨 등을 대체하여 사용할 수 있고 또한 재사용 여부를 판단하기 위하여 수분조절제 6종을 대상으로 특성을 분석하였다. 그 결과 본 실험에 사용한 수분조절제의 종류에 관계없이 유해성분의 함량은 0.0~34.1ppm으로써 농촌 진흥청에서 제시하고 있는 유해성분의 최대함량인 5.0~900.0ppm보다 상당히 적은 것으로 나타나 톱밥 이나 왕겨 등을 대체가 가능할 것으로 판단되었다. 그리고 수분조절제 원상태의 함수율은 최소 12.4% 에서 최대 16.6%까지 측정되어 적정 함수율인 60%이하 보다는 낮아 수분조절제로서의 기능이 충분한 것으로 나타났다. 흡수율은 최소 31.9%에서 최대 600.0%까지 나타나 수분조절제의 종류에 따라 큰 차 이를 보였지만, 가장 적절한 수분조절제는 우드펠릿인 것으로 나타났다. 우드브리켓만 보면, A사 제품 이 가장 높게 나타났고 그 다음으로 C사 및 B사 제품 순으로 나타났다. 전단강도는 전단강도를 측정할 수 없었던 재료를 제외한 5가지 종류에서 원상태의 경우, 평균 271.7N정도로서 가장 크게 나타났다. 그 다음으로 건조 및 흡수상태로서 평균 전단강도가 각각 78.0N 및 27.7N정도로 나타났다. 우드브리켓의 경우, A사 제품이 B, C사 제품보다 상대적으로 전단강도가 현저히 작게 나타났다. 전체적으로 볼 때, 전단강도는 흡수율이 작으면 상대적으로 크게 나타나는 경향이 있었다. 특히 소나무 우드칩이나 참나무 절단목의 경우, 흡수상태에서도 전단강도가 다른 재료에 비해 상대적으로 크기 때문에 돈분과의 교반 시 어느 정도의 동력을 견딜 수 있을 것으로 나타났다.

Hermetia illucens is one of many of solider flies and the species was decomposing ability to food waste, livestock feces and so on. Specially, the study was conducted to investigated of artificial feeding using the livestock feces, because of easy to get and see in a rural areas. But, comparing food waste with livestock feces, the growth character of H. illucens was generally small size and the growth period is longer than feeding food waste. In order this problem, the experiment was performed by mixing sugar 1%, 5%, 10%, 20% , which can accelerate the degradation of the pig and cattle feces during the 0, 3, 5, 7 days. As a result, when the width and length of H. illucens were compared by sugar mixing and fermentation period, they was no significant difference. Decomposing ability of a larvae were 0.44g with cattle feces+sugar 5% than 20% and were 0.64g with pig feces+sugar 20% than 5%.

In this study, the odor generated in a livestock farm with 500 heads of finisher breed in 661 m² was monitored during 6 months using a gas sensor, a wired / wireless communication system and database server. Odor unit, ammonia, hydrogen sulfide, and total volatile organic compounds (TVOC) were monitored using the gas sensor. To show the tendency of odorous substances generation, the odor concentration was shown in the graph on a monthly and daily basis. Among the analysis items, the maximum generation of odor was found to be closely related to the generation of hydrogen sulfide. Through observing the daily and monthly trends of odor substances, it was found that each substance was a useful indicator for monitoring odor, because ammonia, hydrogen sulfide, odor and TVOC were increased and decreased in a similar pattern. The odors were highest in the hours of the early morning (00:00-05:00), the evening (18:00-23:00), and the morning (06:00-11:00) in a day. After the use of the microbial agent was discontinued in autumn (October), anaerobic digestion of the manure in a pit proceeded and the amount of hydrogen sulfide increased. Therefore, despite a slight decrease in ammonia production, the odor unit level did not decrease after October but rather was somewhat increased. In the future, the use of the odor monitoring system is expected to improve the efficiency of odor sources management.

The annual total phosphorus load caused by public wastewater, nightsoil and livestock manure treatment facilities in Korea has been examined macroscopically. Annual domestic average phosphorus (P) inflows through the income of phosphate rock for the last five years (2012 - 2016) were analyzed as 76,598 tons/year. As of the year 2015, the total loadings of phosphorus attributed to public wastewater treatment facilities, nightsoil treatment facilities and livestock wastewater were estimated as 30,269 tons/year, 1,909 tons/year and 18,138 tons/year, respectively. Considering the amount of phosphorus imports, the annual phosphorus load from wastewater, livestock wastewater and excretions is equivalent to 39.5%, 23.7%, 2.5% and totally 65.7%(39.5% + 23.7% + 2.5%). Therefore, the introduction of phosphorus recovery and recycling processes for the public wastewater and livestock manure treatment facilities has been found to be effective because it could reduce the import amount of phosphate rock by up to 60% or more.

본 연구에서는 소비자의 식생활 라이프 스타일 유형에 따른 가축질병 인식(우려도)에 대한 결정요인을 분석하였다. 우선 식생활 라이프 스타일과 관련된 23개의 문항들을 이용하여 요인분석을 실시하고, 요인들의 평균값을 기준으로 군집분석을 실시하였다. 또한, 소비자들의 유형적 특성을 규명하기 위해, 인구 사회경제적 요인 및 식생활 관련 특성을 활용하여 교차분석 및 로짓분석을 실시하였다. 분석결과 가축질병이 국내에서 발생하거나 해외에서 감염 환자가 발생하는 등 사회적 이슈가 되었을 때, 소비자들의 불안감은 소비에 부정적으로 작용할 가능성이 높은 것으로 나타났다. 따라서 가축질병에 대한 소비자의 필요 이상의 불안감은 효율적으로 완화시킬 필요가 있으며 이를 위해 효율적인 리스크 커뮤니케이션을 전개가 필요한 것으로 판단된다.

Chlorine dioxide gas is a relatively new sanitizer in the food industry and has more accessibility than its aqueous form. Depending on the generation method of ClO2, there can be byproducts like chlorite and chlorate ions that can decrease the disinfectant efficacy and purity of ClO2. Recently, a new technology has been developed that generates chlorine dioxide without using chlorine gas. This new electrochemical method generates gaseous chlorine dioxide from aqueous sodium chlorite (NaClO2). Unlike earlier methods, there is reduced generation of byproducts, including chlorite and chlorate. Additionally, the purity of ClO2 obtained by this method can be as high as 98%. The aim of this study was to evaluate the effect of ClO2 gas, generated by the electrochemical method, against the foodborne microorganisms occurring on slaughter equipment and livestock carcasses. Using AISI 304 stainless steel in livestock processing equipment, the disinfectant effect of chlorine dioxide gas, in presence of organic matter such as yeast extract and feces, on E. coli and S. typhimurium contamination, was examined. Both E. coli and S. typhimurium counts were reduced by more than 5 log cycles in presence of 2.5% and 5% feces. When beef, pork skin, and chicken wings were treated with chlorine dioxide gas, despite significant differences in comparison with the control group, the microbial count was reduced by less than 2 log cycles. Overall, our results confirmed the applicability of gaseous chlorine dioxide as a disinfectant in livestock processing equipment and livestock products.

The aim of the present work was to develop simultaneous methods of quantification of carazolol, azaperone, and azaperol residues in livestock and fishery products using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Samples were extracted from beef, pork, chicken, egg, milk and shrimp using acetonitrile (ACN); while flat fish and eel were extracted using 80% ACN. For purification, ACN saturated n-hexane was used to remove fat composition. The standard calibration curves showed good linearity as correlation coefficients; r 2 was > 0.99. Average recoveries expressed were within the range of 67.9-105% for samples fortified at three different levels (0.5 × MRL, 1 × MRL and 2 × MRL). The correlation coefficient expressed as precision was within the range of 0.55- 7.93%. The limit of quantification (LOQ) was 0.0002-0.002 mg/kg. The proposed analytical method showed high accuracy and acceptable sensitivity based on Codex guideline requirements (CAC/GL71-2009). This method can be used to analyze the residue of carazolol, azaperone, and azaperol in livestock and fishery products.

As of 2014, 26.4% of the total regulated odor emission facilities are occupied by livestock facilities. The odor of pigs is 10.9 OU·m3 / min per pig, which generates 15-50 times higher odor than other livestock. It is also a major cause of livestock odor complaint. Livestock odor substance is mixed 169 kinds of ingredients, 30 of which can be detected as odor. It contains sulfur, volatile fatty acids, phenols and indoles, ammonia and volatile amines. In particular, odorous substances of phenols and indole derivatives not included in domestic designated odor substances have high odor contribution and are not well decomposed. Therefore, it is known that despite the use of the odor reducing agent having a high removal rate of ammonia and the like, the residue is long and causes continuous discomfort. The odor problem using physical and chemical methods can not be solved because it can not solve the fundamental problem if the animal odor is not decomposed or removed. In the anaerobic environment, the bacteria present in the manure may produce volatile organic compounds, which are the cause of the odor, and the odor may be generated, and some microorganisms decomposing the odor substances may reduce the odor. B. subtillis, Saccharomyces cerevisiae, L. acidophillus, Enterococcus faecium, L. plantarum, B.coagulans, B. fermentum, B. thuringiensis, B. licheniormis, B. subtillis, Enterococcus faecium, Lactobacillus acidophllus, L. fermentum, L. lactis, L. plantarum, L. casei, L. brevis, Streptococcus faecium, Clostridium butyricum, Saccharomyces cerevisiae, Aspergillus niger, A. oryzae, and photosynthetic bacteria are used as odor-reducing microorganisms.

가축질병 바이러스는 매우 빠르게 전파되는 특징을 가지고 있으며 질병에 걸린 동물의 분비물에 접촉하거 나, 사람이나 차량에 의한 바이러스의 이동, 또는 공기를 통하여 전파가 이루어진다. 본 연구에서는 가축 및 분뇨 운반차량이 질병을 전파시키는 요인이 될 수 있다고 판단하고 전염병 발생 시 질병의 이동 경로를 파악 하고 효율적인 방역범위를 설정하는데 기초자료로 활용 하고자 한다. 축산차량의 이동경로를 분석하기 위해 ArcGIS(Ver. 10.3, ESRI, USA)의 Spatial Statistics tool에서 Measuring geographic distribution방법을 활용하여 Directional distribution을 추출하였다. 결과 값으로 위도(Latitude), 경도(Longitude), Rotation, XstdDist, YstdDist을 얻었다. 그 결과 특정한 목적을 가지고 출발지가 일정 지역에 귀속되어 있는 가축 및 분뇨 차량의 경우 출발지점, 도착지점, 목적지가 크게 바뀌지 않을 경우, 이용하는 도로는 한정되어 있었다. 30대의 차량 중 대다수의 차량이 일정한 패턴을 가지고 평상 시 이용하는 경로만 이용하여 분뇨를 운반하는 것을 확인 할 수 있었다. 본 연구를 통해 전염병 발생 시 질병의 이동 경로를 파악하고 효율적인 방역범위를 설정하여 투입 인원과 장비를 줄이면서 현재보다 효율적인 방역 범위를 설정이 가능할 것으로 판단되었다.

In this paper, a mathematical model of regionalization based on graph theory to investigate the patterns induced by movements of livestock vehicles in cities under outbreaks of highly pathogenic avian influenza (HPAI) is proposed. We then compare the results of simulation from the regionalization model to actual HPAI outbreaks in 2016/2017 to evaluate the validity of the model. Specifically, we (1) configured a complex network structure with analytic tools and properties in graph theory to abstract the paths among farms and livestock facilities; (2) employed statistical methods to estimate the possibility of propagation between two clusters; (3) applied the developed method to an actual HPAI outbreak in Korea in 2016 and conducted a simulation to determine if the proposed modeling for regionalization is an effective prediction measure. The clustered regions proposed by the simulation correctly reflected the regional clustering of actual cases, while simultaneously contain the cities exposed to potential damage when separated. Based on these findings, we conclude that our proposed regionalization model is suitable for making policy judgments to establish a preemptive biosecurity system.

It became possible to perform genomic predictions using single nucleotide polymorphism (SNP) with advancements in genomics technology, not only in human but in livestock as well. There are strong interests in improving economical traits in livestock through identifying causative mutation, genes or predicting genomic breeding values. We present the current status of genome prediction studies for phenotype estimation of economic traits in livestock from various perspectives based on the genomic area. First, we introduce theoretical background of genomic prediction methods and newest development on SNP information. Thanks to develop sequencing technology, multi-omics data can be used to predict phenotypes associated with the economic traits. In particular, many studies show that genomic prediction accuracy of genomic partitioning data based on the biological information is higher than that of commercial SNP chip. Therefore, multi-omics data can be useful for genomic prediction studies. It is also important that researchers should consider factors affecting genomic prediction accuracy such as heritability, Quantitative Trait Loci (QTL) and marker density, size and structure of reference population. We also introduce genomic prediction studies based on the integration of multi-omics data that shows improvement of prediction accuracy than typical Genomic Best Linear Unbiased Prediction (GBLUP) models. We concluded that genomic prediction studies can be expanded to apply social issues, new phenotypes, or precision agriculture such as diseases, climate change, and metabolism including economic traits with multi-omics data using high-throughput technologies.

This study evaluated the ecotoxicological properties of livestock waste water treated by a LID (Low Impact Development) system, using a mixture of bio-reeds and bio-ceramics as suitable bed media for a subsequent treatment process of a livestock wastewater treatment plant. The relationship between the pollutant reduction rate and the ecotoxicity was analyzed with the effluents from the inlet pilot plant, with vegetated swale and wetlands and the batch type of an infiltration trench. Each pilot plant consisted of a bio process using bio-reeds and bioceramics as bed media, as well as a general process using general reeds and a bed as a control group. The results indicated that, after applying the HRT 24 hour LID method, the ecotoxicity was considerably lowered and the batch type pilot plant was shown to be effective for toxicity reduction. The LID method is expected to be effective for water quality management, considering ecotoxicity by not only as a nonpoint source pollution abatement facility but also, as a subsequent treatment process linked with a livestock manure purification facility. It is necessary to take the LID technic optimization study further to apply it as a subsequent process for livestock wastewater treatment.

This experiment was conducted to evaluate feed value and usability of soybean varieties as livestock forage. In this study, three soybean cultivars, OT93-26, Geomjeongsaeol, and Pungwon, were harvested at R5 (beginning seed development)- and R6 (full seed)-reproductive stages for analyzing feed value of soybean. Days to R5 stage harvest of OT93-26 among the three soybean cultivars was 55 days and the shortest while Pungwon took 103 days to reach at R6 stage. The R6-harvested soybeans had higher dry matter (DM) yields and crude protein (CP) content than the R5-harvested. However, both DM and CP were the highest in the R6-harvested Geomjeongsaeol. Contents of neutral detergent fiber (nDF) and acid detergent fiber (ADF) of Pungwon harvested at R5 were the highest whereas the R6-harvested Geomjeongsaeol had the lowest. Digestible dry matter (DDM), dry matter intake (DMI), and relative feed value (RFV) of the R6-harvested Geomjeongsaeol and Pungwon were higher than those of the R5-harvested, but in case of OT93-26, those at R6 stage were low rather than those at R5 stage. However, soybean could be used as alternative forage with high feed value for livestock. Taken together, Geomjeongsaeol could be used for developing new forage soybean varieties with high feed value, and R6 would be the optimum harvesting stage for yield and quality of forage soybean.

In attempt to avoid crop damage through wild bird’s picking, this study was designed with aim to evaluate several pre-sowing soybean seed coatings for optimum yield in corn-soybean mixed forage. It was investigated under four cropping treatments, viz. 1) corn sole, 2) corn mixed with soybean without any coating, 3) corn with iron coated soybean and 4) corn with thiram coated soybean. Each treatment had three replicates and corn sole was control treatment. Pioneer (P1184) and crossbred (PI483463 × Hutcheson) seeds were used for corn and soybean, respectively. The trial was conducted under randomized block design from 5th June to 23rd September, 2015. Data were an alyzed through ANOVA technique using SAS9.1.3 software. Results depicted that survivability of soybean against wild birds damage was found better (p<0.05) in thiram coating which was higher than iron coating and control treatment but later on thiram coating had adverse effects on subsequent growth of soybean plants. Corn stalk height was decreased (p<0.05) in thiram coating, whereas corn ear height was reduced in iron coating treatment. Iron coating enhanced (p<0.05) height of soybean plant (p<0.05) better than that of thiram coating. Soybean seed coatings didn’t influence dry matter yield and nutritive value in terms of total digestible nutrients yield in corn soybean mixed forage. Conclusively, although presowing thiram coating enhanced survivability of soybean plants against wild bird damage but had adverse effects on its subsequent growth. However, soybean seed coatings didn’t influence yield and nutritive value of corn soybean intercropping forage

Recently, in Korea various kinds of genetically modified (GM) crops have been imported and used as a raw material to manufacture foods and livestock feeds, but the different social concerns about the benefits and the potential risks of GM crops are being shown with a different reaction from the public. Thus a persistent management is required for the safe utilization of genetically modified organism (GMO). PCR analysis of transgene into crop is generally performed for the efficient post management of GMOs. The most important prerequisite for the application of nucleic acid detections is to decide the effective DNA-extraction methods. Particularly, in the case of processed feeds, the nucleic acids of which may be damaged by heating, high pressure, pH treatments, fermentation, etc. in processing, DNA must be extracted with high sensitivity from the samples to perform the PCR successfully. In this study, seven of DNA-extraction methods used commercially and non-commercially were compared with respect to the yields and quality of DNA extracted from livestock feeds and those crop materials. Amounts of genomic DNA obtained from the extraction methods varies according to feed configurations and crop materials. The DNA yield and uniformity of samples extracted with PG, CTAB, and QF method is greater than that obtained from other extraction methods. In the DNA integrity of the selected extraction methods, PCR analysis showed distinct amplifications and similar patterns in detecting crop endogenous genes and GMO genes. These results would be applicable for the selection of an adequate DNA-extraction method in extracting processed feeds and/or crop materials.