치자 껍질의 70% methanol, ethyl acetate (EA) 및 distilled water (DW)의 용매 별 추출물 의 total phenol 함량 및 질소 산화물 소거능, 환원력, β-carotene 탈색을 이용한 항산화력 및 지질과 산화 저해능 측정을 통하여 치자의 기능성 식품 소재로서의 가치를 검토한 결과, anthocyanin 함량은 3.519±0.635 mg/100 g DW로 나타났으며, 용매 별 추출 수율은 DW (39.87%), 70% methanol (36.26%), EA (2.88%) 로 관찰되었다. 추출 용매 별 항산화 활성은 농도(0.2, 0.4, 0.6 mg/mL)가 증가 할수록 유의적으로 증가하였으며 control로 사용된 ascorbic acid, BHA, trolox 보다는 낮은 활성이 확 인되었다. 치자 껍질의 total phenol 함량은 EA, 70% methanol, DW 추출물 순으로 EA 추출물에서 26.59±0.20 CAE (caffeic acid equivalents) mg/g으로 가장 높았으며, Nitric oxide (NO) radical 소거 능에서는 70% methanol (70.32~76.15%), DW (52.66~59.31%), EA (34.65~46.98%) 추출물 순으로 나타났다. Nitrite (NO2) 소거능은 70% methanol (34.57~39.33%), DW (32.53~38.47%), EA (27.59~32.62%) 순으로 관찰되었다. β-carotene 탈색 저해능은 DW (41.55~50.97%), 70% methanol (23.37~44.80%), EA (13.37~25.24%) 순으로 동정되었다. Reducing power (optical density)는 70% methanol (0.044~0.127), DW (0.033~0.099), EA (0.026~0.097) 순으로 확인되었다. 지질과산화 저해 능은 껍질 추출물 중 70% methanol (56.51~76.21%), EA (54.59~63.34%), DW (47.92~61.11%) 순 으로 관찰되었다(p<0.05). 이에, 치자 껍질은 기능성 식품 및 천연 항산화제로서의 가치가 매우 높을 것 으로 판단된다.

이 연구는 최근 37년(1977-2013) 동안 여름철 동중국해에 영향을 준 태풍빈도와 북서태평양 몬순지수와의 상관 을 분석하였다. 두 변수 사이에는 뚜렷한 양의 상관관계가 존재하였으며, 엘니뇨-남방진동 해를 제외하여도 높은 양의 상관관계는 변하지 않았다. 이러한 두 변수 사이에 양의 상관관계의 원인을 알아보기 위해 가장 높은 북서태평양 몬순 지수를 갖는 11개 해(양의 북서태평양 몬순지수 위상)와 가장 낮은 북서태평양 몬순지수를 갖는 11개 해(음의 북서태평 양 몬순지수 위상)를 선정하여 두 위상 사이에 평균 차를 분석하였다. 양의 북서태평양 몬순지수 위상에는 태풍들이 열대 및 아열대 서태평양의 동쪽해역으로부터 동중국해를 지나 한국 및 일본을 향해 북상하는 경향을 나타내었다. 음의 북서태평양 몬순지수 위상에는 태풍들이 남중국해를 지나 중국 남부지역을 향해 서진하는 패턴을 보였다. 따라서 동아 시아 중위도까지 먼 거리를 이동하면서 바다로부터 충분한 에너지를 얻을 수 있는 양의 북서태평양 몬순지수 위상에의 태풍강도가 더 강하였다. 또한 양의 북서태평양 몬순지수 위상에 태풍들이 더 많이 발생하는 특성을 보였다. 850 hPa과 500 hPa에서의 수평 대기순환에 대한 두 위상 사이에 차에서는 열대 및 아열대 서태평양에서 저기압 아노말리가, 동아 시아 중위도 지역에는 고기압 아노말리가 강화되었다. 이 두 기압계 아노말리로 인해 동중국해에서는 남동풍 아노말리 가 발달하였으며, 이 남동풍 아노말리가 태풍들을 동중국해로 향하게 하는 지향류 아노말리의 역할을 하였다. 또한 열 대 및 아열대 서태평양에서 발달한 저기압 아노말리로 인해 양의 북서태평양 몬순지수 위상에 태풍들이 좀 더 많이 발 생할 수 있었다.

본연구는 콜라비 추출물의 항산화 및 항염 효능을 조사하기 위하여 수행하였다. 콜라비는 70% 에탄올을 이용하여 조추출한 후 헥산, 에틸아세테이트, 부탄올을 이용하여 용매 극성에 따라 순 차적으로 분획하였다. 항산화 활성은 총폴리페놀 함량 측정과 ABTS 라디칼 소거활성을 측정하여 평 가하였다. 에틸아세테이트 분획물이 총폴리페놀 함량(27.33±0.26 mg GAE/g)과 ABTS 라디칼 소거활 성(IC50 172.9±1.6 ㎍/mL)이 가장 높게 측정되었다. 항염 활성은 RAW 264.7 세포를 이용하였으며, 에 틸아세테이트 분획물이 가장 높은 항염 활성을 보였다. 에틸아세테이트 분획물의 LPS로 유도된 RAW 264.7 세포에서 전염증성 매개인자들에 대한 저해효과를 농도별로 측정하여 확인하였다. 콜라비 에틸 아세테이트 분획물은 NO, PGE2 생성과 iNOS와 COX-2 및 TNF-α, IL-6, IL-1β와 같은 전염증 성 사이토카인들의 단백질 발현을 농도의존적으로 저해하였다. 이러한 결과들은 콜라비가 항산화 및 항염 효능을 가지는 소재로서의 개발 가능성이 있음을 시사한다.

Information about time spent in microenvironments plays a critical role in exposure assessment of the person concerned, considering that personal exposure relies on the characterization of time-activity patterns of the population at risk as human activities impact the timing, location, and level of personal pollutant exposure. The purpose of this paper was to present the time-activity patterns of Korean adults from a population-based study, and to determine the time spent each day in microenvironments. The population-based study collected time-activity data of about 46,000 adults for two consecutive days on weekdays (n=28,152) and weekends (n=18,800). The mean times spent at one’s own house, workplace or school, other’s house house, restaurant or bar, other places, and transportation related to the whole sample of 28,152 people were 14.90 hrs (62.08%), 4.28 hrs (17.81%), 0.24 hrs (1.01%), 0.51 hrs (2.14%), 2.45 hrs (10.21%), and 1.62 hrs (6.74%) on weekdays, respectively. Looking at the total time spent indoors by adults in different countries, the following four countries were ranked as follows, from lowest to highest: USA > Canada > Korea > Hong Kong. Due to cultural, socioeconomic and climatic differences, it may not be appropriate to directly apply statistical data of other countries to the Korean situation. This paper provides information on how the proportion of persons in different locations changes by time of day, on weekdays and weekends. Here, we can see that over 90% of respondents were in a residence from about 11 PM to 5 AM, and the largest proportion of respondents in workplaces or schools and other places is found between 8 AM and 5 PM.

This study was carried out to investigate antimicrobial activity and characteristics of Asparagus cochinchinenesis which was steamed and fermented with lactic acid bacteria. A. cochinchinensis was prepared to steaming process which was washed and freeze dried. A. cochinchinensis was steamed at 95oC for 12 h and dried by hot air at 50oC for 24 h. After steaming process, A. cochinchinensis was fermented with lactic acid bacteria (Leuconostoc mesenteroides 4395, Lactobacillus sakei 383 and Lactobacillus plantarum KCCM 11322). Ethyl acetate extracts of fermented A. cochinchinensis had antimicrobial activities for the respiratory disease bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli). A. cochinchinensis had highest antimicrobial activity for the P. aeruginosa which fermented with L. mesenteroides 4395. The minimum inhibition concentration (MIC) of A. cochinchinensis fermented with L. mesenteroides 4395 was 10 mg/mL for S. aureus, S. epidermidis, E. coli and 5 mg/mL for P. aeruginosa. The MIC of A. cochinchinensis fermented with L. sakei 383 and A. cochinchinensis fermented with L. plantarum KCCM 11322 were the same. Total sugar was decreased from 863.33±17.47 mg/mL to 722.67±5.51 mg/mL during the steaming process. But reducing sugar was increased from 99.36±1.32 mg/mL to 109.29±2.71 mg/mL during the steaming process. Total sugar was decreased to 301.50-361.42 mg/mL and reducing sugar was decreased to 27.39-62.20 mg/mL during the fermentation process.

This study was carried out to investigate antimicrobial activity and characteristics of Asparagus cochinchinenesis which was steamed and fermented with lactic acid bacteria. A. cochinchinensis was prepared to steaming process which was washed and freeze dried. A. cochinchinensis was steamed at 95oC for 12 h and dried by hot air at 50oC for 24 h. After steaming process, A. cochinchinensis was fermented with lactic acid bacteria (Leuconostoc mesenteroides 4395, Lactobacillus sakei 383 and Lactobacillus plantarum KCCM 11322). Ethyl acetate extracts of fermented A. cochinchinensis had antimicrobial activities for the respiratory disease bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli). A. cochinchinensis had highest antimicrobial activity for the P. aeruginosa which fermented with L. mesenteroides 4395. The minimum inhibition concentration (MIC) of A. cochinchinensis fermented with L. mesenteroides 4395 was 10 mg/mL for S. aureus, S. epidermidis, E. coli and 5 mg/mL for P. aeruginosa. The MIC of A. cochinchinensis fermented with L. sakei 383 and A. cochinchinensis fermented with L. plantarum KCCM 11322 were the same. Total sugar was decreased from 863.33±17.47 mg/mL to 722.67±5.51 mg/mL during the steaming process. But reducing sugar was increased from 99.36±1.32 mg/mL to 109.29±2.71 mg/mL during the steaming process. Total sugar was decreased to 301.50-361.42 mg/mL and reducing sugar was decreased to 27.39-62.20 mg/mL during the fermentation process.

Basic dyes such as malachite green and methylene blue have been used as disinfectants to control water fungal infections since the 1930s. However, after succeeding reports of carcinogenicity and bioaccumulation of the dye, their use was forbidden in lieu of public health. This study undertook to evaluate the therapeutic effect of sulfur solution processed by effective microorganisms (EM-PSS) against Saprolegnia parasitica infection, and its safety in fish. In vitro antifungal evaluation of EM-PSS inhibited the growth of S. parasitica mycelia at concentrations of 50 ppm or higher. The acute toxicity test of EM-PSS to the mud fish (Misgurnus mizolepis) measured a no effect concentration (NOEC) at 100 ppm, the lowest effect concentration (LOEC) at 125 ppm, and the half-lethal concentration (LC50) at 125 ppm in juvenile and 250 ppm in the immature stage. In addition, the ecotoxicity test of EM-PSS using Daphnia magna inhibited swimming of D. magna at concentrations of 100 ppm or less. Lastly, the EM-PSS prevented infection of S. parasitica to mud fish, at concentrations of 50 ppm. Furthermore, at 100 ppm concentration, the EM-PSS showed no acute toxicity on mud fish, nor any eco-toxic effects on D. magnano. Therefore, we conclude that carcinogenic disinfectants such as malachite green and methylene blue could be replaced by EM-PSS to remove S. parasitica in mud fish farming, and might be a potential eco-friendly disinfectant in aquaculture.

Alginate lyase from Streptomyces violaceoruber was purified by DEAE sephacel chromatography and SP sepharose chromatography. The specific activity of the purified enzyme was 14.6 units/mg protein, representing a 40.6-fold purification of the crude extract. The final preparation thus obtained showed a single band on Tricine-SDS polyacrylamide gel electrophoresis whose molecular weight was determined to be 23.3 kDa. The polyMG block of sodium alginate was hydrolyzed by the purified alginate lyase and then separated by activated carbon column chromatography and bio gel P-2 gel filtration. The main hydrolysates were composed of hetero type M/G-oligosaccharides with the degrees of polymerization (D.P.) being 6 and 8. To investigate the effects of hetero type M/Goligosaccharides from the sodium alginate on the growth of some intestinal bacteria, cells were cultivated individually on the modified-MRS medium containing D.P. 6 and 8 M/G-oligosaccharides. B. longumgrew 4.25-fold and 6.44-fold more effectively by the treatment of D.P. 6 and 8 M/G-oligosaccharides compared with those of standard MRS medium. In addition, B. bifidumgrew 3.3-fold and 5.4-fold more effectively by the treatment of D.P. 6 and 8 M/G-oligosaccharides. In conclusion, D.P. 8 was more effective than D.P. 6 hetero M/G-oligosaccharides as regards the growth of Bifidobacteriumspp. and Lactobacillus spp

Alginate lyase from Streptomyces violaceoruber was purified by DEAE sephacel chromatography and SP sepharose chromatography. The specific activity of the purified enzyme was 14.6 units/mg protein, representing a 40.6-fold purification of the crude extract. The final preparation thus obtained showed a single band on Tricine-SDS polyacrylamide gel electrophoresis whose molecular weight was determined to be 23.3 kDa. The polyMG block of sodium alginate was hydrolyzed by the purified alginate lyase and then separated by activated carbon column chromatography and bio gel P-2 gel filtration. The main hydrolysates were composed of hetero type M/G-oligosaccharides with the degrees of polymerization (D.P.) being 6 and 8. To investigate the effects of hetero type M/Goligosaccharides from the sodium alginate on the growth of some intestinal bacteria, cells were cultivated individually on the modified-MRS medium containing D.P. 6 and 8 M/G-oligosaccharides. B. longumgrew 4.25-fold and 6.44-fold more effectively by the treatment of D.P. 6 and 8 M/G-oligosaccharides compared with those of standard MRS medium. In addition, B. bifidumgrew 3.3-fold and 5.4-fold more effectively by the treatment of D.P. 6 and 8 M/G-oligosaccharides. In conclusion, D.P. 8 was more effective than D.P. 6 hetero M/G-oligosaccharides as regards the growth of Bifidobacteriumspp. and Lactobacillus spp. Key words: hetero M/G-oligosaccharides, Streptomyces violaceoruber

본고에서는 1688년 57세의 나이로 사제 서품을 받고 上海에서 10년간 선교활동(‘十年海上’) 을 수행한 예수회 신부 오어산의 삶을 통해 동질적인 가치의 보편성을 추구하는 가톨릭과 유 교문화를 중심으로 하는 중국 고유문화 사이에서 발생하는 긴장을 해결하고, 두 문화를 융합 하기 위한 그의 노력을 살펴보고자 하였다. 이를 위해 오어산의 三餘集 속에 수록된 시 가운데 상해에서 창작한 기독교문화와 예수회 신부들의 활동을 담아낸 광의의 天學詩를 분석 대상으로 하였다. 본문에서는 먼저 상해지역의 기독교 전교기지였던 老天主堂을 중심으로 한 예수회 오어산 신부의 생활을 살펴보고, 예수회원의 중국 선교활동에 있어 중요한 역할을 담 당했던 서양의 천문학과 자연과학지식에 대한 그의 태도를 밝히고자 하였다. 이를 통해 예수 회 신부가 되면서 자연스럽게 바티칸 포교성의 지휘와 감독을 받게 된 중국 지식인의 글로벌한 종교 활동을 살펴볼 수 있었다. 다음으로 오어산 신부가 상해지역의 선교활동에서 보여준 목자로서의 노력, 사람 낚는 어부가 되고자 한 선교사로서의 모습을 살펴보고자 하였다. 이를 통해 기독교와 중국 고유문화를 융합하고자 노력한 예수회 신부 오어산의 사목활동이 갖는 로컬적인 의미를 확인할 수 있었다.

본 연구에서는 약용식물 추출물을 함유한 숙취해소 음료가 항산화 및 알코올 분해에 미치는 영향을 조사하기 위하여, 약용식물 추출물의 총 페놀성화합물 함량, 총 플라보노이드 함량 및 DPPH radical 소거활성 및 ALDH(aldehyde dehydrogenase) 활성을 측정하였고, 약용식물 추출물을 함유한 숙취해소 음료의 복용이 음주 후 호흡 중 알코올 농도에 미치는 영향을 조사하였다. 항산화활성은 빼빼목 추출물 에서 높은 수치(total phenolic compound 97.5.6mg/g, total flavonoid 306.5mg/g, DPPH radical 소거능 80.1%)를 나타냈으며 Acetaldehyde(ALDH)에 대한 활성은 헛개 및 울금 추출물에서 306% 및 292%로 가장 높은 활성을 나타내어, 헛개 및 울금 추출물이 ALDH 제거에 효과적인 것으로 나타났다. 헛개 및 울금 추출물이 함유된 숙취해소 음료를 이용하여, 임상실험을 실시한 결과, 시간경과에 따라 대조군에 비해 숙취해소음료 섭취군에서 알코올 농도가 감소되는 경향을 나타냈으며 흡연자에 비해 비 흡연자가, 남성에 비해 여성에게 숙취해소 음료의 섭취가 더 효과적인 것으로 확인되었다.

The purpose of this study was to investigate how the parent’s game addiction has an effect on children’s physical activity, physical self-conception was used by medium. Participants were 230 mothers with children between 5 and 7 years old. One-hundred eighty surveys, excluding those with omitted or odd answers, were analyzed. The data were analyzed with descriptive statistics, bivariate correlation analysis, path analysis using WEB-R 3.0(LAVAAN). The first, as the result indicated, parent’s game addiction affected the children’s physical activity directly. The second, parent’s game addiction affected the children’s physical self-conception. The third, children’s physical self-conception. affected the children’s physical activity. Therefore the effort to increase the children’s physical self-conception may contribute to developing children’s physical activity.

To determine the medicinal properties of Pleurotus cornucopiae and Safflower Oil (PS), the osteoblast effect was investigated. PS can prevent estrogen deficiency-induced bone loss. It was taken the measurements of biochemical factors such as serum osteocalcin, alkaline phosphatase, IGF-1, and bone status. Forty adult female Sprague-Dawley rats were randomly assigned to four equal groups for 12 weeks of treatment: (1) sham operation (SHAM) + vehicle; (2) ovariectomy (OVX) + vehicle; (3) OVX + 17β-estradiol (25 μg/kg); (4) OVX + PS (300 mg/kg/d, PO). At the end of the experiment, bone turnover, and trabecular microarchitecture were assessed by micro-computed tomography (micro-CT), and histopathology, respectively. Ovariectomized rats experienced remarkable increases in global volumetric bone density, and trabecular microarchitecture deterioration. These OVX-induced pathological changes are reversible in that most of them could be mostly corrected upon 17β-estradiol treatment. PNS treatment significantly prevented bone mass loss and microarchitecture deterioration. Moreover, PS enhanced osteoblast activity but suppressed osteoclast turnover, as evidenced by decreased levels of serum C-terminal telopeptides of type I collagen and elevated levels of alkaline phosphatase. PS mitigates estrogen deficiency-induced deterioration of trabecular microarchitecture.

2015 교육과정은 핵심역량 중심의 교육과정이다. 본고는 학습자들의 핵심역량을 기르기 위해, 한 문 교과서 한시 단원이 무슨 내용을 어떤 방식으로 기술하여야 할지에 대해 살펴보았다. 먼저 2015 개정 한문과 교육과정에 나타난 한시 학습 내용과 방법을 분석하였다. 이어, 2009 개 정 교육과정에 따른 중학교 한시 단원을 분석하되, 교과서에 제시된 학습목표, 학습활동을 중심으로 살펴보았다. 셋째, 앞의 두 자료 분석을 바탕으로, 핵심역량 중심의 교과서 한시 단원 집필을 위해 몇 가지 제안을 하였다. 학습 내용 면에서는 한시 형식 및 한시 풀이하기 내용 진술이 추가되어야 한다고 하였다. 한시 교과서 기술 방식에 있어서, 학습자들이 학습 과정에 참여할 수 있도록 교과서 학습목표 설정과 위계화, 학습자들이 직접 사고하여 채울 수 있는 적절한 여백. 교수-학습 단계별 적절한 활동의 효과적 설정과 배치가 필요하다고 제안하였다. 마지막으로, 한시 교과서 구성 안을 제시하였다.

As the hangover relieving drink market has been expanded, there have been efforts to produce traditional drinks that can be easily taken through reducing material costs after finding materials with high alcohol-degrading activity from agricultural products and manufacturing drinks in a way to produce sikhye. Studies were made to know quality, the ability to relieve hangover and preference of drinks for which seven kinds of agricultural products, including shiitake mushrooms, were added in the forms of fresh juice, extracts and powder. Farm products showed the highest acetaldehyde dehydrogenase (ALDH) when they were added in the form of powder before saccharification and shiitake mushrooms showed the highest enzyme activity when they were added in the form of powder. When added in the form of powder, people showed the lowest preference except when radish and hericium erinaceum were added. When bear puree was added to a mixture of powdered shiitake mushrooms, radish, bean sprouts and dadagi cucumber, enzyme activity got higher and people showed higher preference. An experiment of animal behaviors showed that the mixture had a high ability to relieve hangover in one or two hours after 10% or 40% alcohol was orally administered to mice due to long traveling distance of mice.