Environmental stresses caused by climate change, such as high temperature, drought and salinity severely impact plant growth and productivity. Among these factors, high temperature stress will become more severe during summer. In this study, we examined physiological and molecular responses of maize plants to high temperature stress during summer. Highest level of H2O2 was observed in maize leaves collected July 26 compared with June 25 and July 12. Results indicated that high temperature stress triggers production of reactive oxygen species (ROS) in maize leaves. In addition, photosynthetic efficiency (Fv/Fm) sharply decreased in leaves with increasing air temperatures during the day in the field. RT-PCR analysis of maize plants exposed to high temperatures of during the day in field revealed increased accumulation of mitochondrial and chloroplastic small heat shock protein (HSP) transcripts. Results demonstrate that Fv/Fm values and organelle-localized small HSP gene could be used as physiological and molecular indicators of plants impacted by environmental stresses.

울금(Curcuma longa L.)의 사포닌 함량과, 증류수(distilled water, DW), 70% 에탄올 및 노르말 부탄올의 3가지 용매를 사용하여 산화억제 효과를 통한 기능성 식품 소재로서의 가치를 확인한 결과, 총 사포닌 함량은 7.506±0.349 mg SE/g dry weight으로 확인되었다. 추출 수율은 DW (17.11%), 70% 에탄올(15.26%), 노르말 부탄올(4.12%)로 DW에서 가장 높게 나타났으며, 노르말 부탄올 추출물에서 낮은 수율을 보였다. 항산화 활성은 농도가 높아질수록 활성이 증가되는 것으로 관찰되었다. 총 페놀 함량은 DW, 70% 에탄올, 노르말 부탄올 순으로 각각 8.57, 41.01, 119.11 mg GAE/g의 함량으로 나타났으며, 노르말 부탄올 추출물에서 유의적인 차이를 보이며 높게 관찰되었다(p<0.05). DPPH 라디칼 소거활성은 DW, 70% 에탄올, 노르말 부탄올 순으로 확인되었다. ABTS 라디칼 소거활성, 환원력 및 ferric reducing antioxidant power (FRAP) 또한 DPPH 라디칼 소거활성과 비슷한 양상으로 나타났지만, ferrous ion 킬레이트 능은 노르말 부탄올(15.87~21.17%), 70% 에탄올(26.10~50.76%), DW(44.47~84.24%) 순으로 측정되었다. 이에, 울금은 산화억제 및 생리활성을 가지는 것으로 규명되었으며 기능성 식품 소재로서의 활용 가능성이 높은 것으로 사료된다.

본 연구의 목적은 온도와 토양수분에 따른 마늘의 생육, 생리장해 및 수량에 미치는 영향을 구명하고자 실시 하였다. 실험은 온도가 6℃ 차이가 나는 온실에서 수행 되었는데, 정식부터 수확까지 외기(A)보다 3℃(A+3℃), 6℃(A+6℃) 고온조건, 토양수분은 적습(OI) 대비 수확기 무렵의 다습(EI) 처리를 하였다. 그 결과, 마늘 생육특성은 온도와 토양수분 조건(0.34m3/m3)에 따라 고도의 유의성을 보여 고온일수록 그리고 적습 처리구일수록 컸다. 초장은 외기보다 A+6℃-OI 처리구에서 47.4cm로 가장 컸으며, 엽장과 엽폭 역시 외기보다 A+6℃-OI 처리구가 각각 16.1cm, 2.4cm로 가장 컸다. 마늘재배 기간 중 외기보다 3℃, 6℃ 고온조건이 되면 스펀지마늘 발생율이 높아져 A+6℃-OI 처리구는 12.9% 발생하였고, A-EI 처리구에서는 전혀 발생하지 않았으며, 인편무게와 1쪽당 무게는 A+6℃ 고온구에서 크게 감소하여 수량이 외기 대비 A+6℃ 처리구는 평균 51%, A+3℃ 처리구는 평균 22% 감소하였다. 따라서, 마늘재배시 외기보다 6℃ 고온조건과 다습조건이 되면 상품수량 감소하고, 생리장해 발생이 많아지는 것으로 나타났다.

Products are successfully designed only when they are in accord with the users’ emotional needs. A systematic research approach is aimed to propose that physiological metrics can be effectively used to assess user emotion and behavior intention based on an eye tracker and neurophysiological approach. Forty participants (20 males and 20 females, mean age=35.6, SD=6.38, range 21-48 years), were recruited from college campuses and communities to conduct an eye tracker and electroencephalography (EEG) experiment with the presented stimuli (images of SUVs). The study uses partial least squares structural equation modeling (PLS-SEM) to test the model hypotheses. The results show a strong and significant relationship between eye tracker metrics, neurophysiological metrics, user affective responses, and behavior intention. These findings could enable industrial counselors, professional product designers, and academics to categorize users’ emotional needs that can be subsequently incorporated into final product design.

Aronia is difficult to eat because it has a bitter taste, bitter taste, and sour taste. Because processing is essential for eating, quality information suitable for processing is needed. This experiment was carried out to confirm the step suitable for processing. Four grades of ripening were collected from 3 varieties (Viking, Nero, McKenzie) based on color, and compared with samples stored for 2 weeks after harvesting. Physicochemical properties such as color, hardness, sugar content, pH and acidity, functional components such as total polyphenols and flavonoids, and antioxidative activities such as DPPH and ABTS were investigated. When compared according to varieties, the hardness of the first degree was highest in other degrees, Depending on harvest time, first degree was higher than other degrees and it decreased with harvest time. Varieties was not significant different lightness, redness and yellowness value. compared according to harvest time, redness was the highest in second degree and lightness, yellowness decreased with harvest time. sugar content was highest in Nero and Mckenzie, birx value increased significantly of ripening and increased with harvesting time. pH was found to be highest in Nero, acidity value was the highest in viking. DPPH, ABTS radical scavening were Nero varieties had the highest radial scavening and increased significantly as the ripening degree increased. Total phenol and flavonoid contents were found to be highest in Nero, the highest content was showed at the first degree of harvesting. The most suitable stage for the processing was considered to be the best after post harvest treatments considering brix, hardness and acidity.

본 연구는 NaCl 농도가 수리취의 무기이온 함량, 효소활성, 광합성 반응 및 생장특성에 미치는 영향을 알아보기 위하여 수행되었으며, NaCl 농도는 4가지 처리로 조절하였다(0, 50mM, 100mM, 200mM). NaCl 처리농도가 증가할수록 최대광합성속도, 순양자수율, 탄소고정효율과 같은 광합성 활성이 감소하 였으나 수분이용효율은 증가하였다. 또한 엽면적, 엽건중, 엽면적비, 줄기길이 및 T/R율이 감소하였는 데 특히 100mM NaCl 농도조건에서 크게 감소하였다. 염분스트레스 처리시 이온유출량, H2O2 함량 및 지질과산화(MDA)가 증가하여 세포막의 피해가 커졌으나 염분스트레스에 대응하기 위해 삼투조절물질인 proline 함량도 유의적으로 증가하였고, 산화적 스트레스를 소거하기 위해 APX와 GR의 활성 역시 증가하였다. NaCl 농도가 증가할수록 엽과 뿌리의 Na 함량도 증가하였으나, Ca, K 및 Mg 함량은 이와 반대되는 경향을 보였다. 결과적으로 수리취는 비교적 염분 스트레스에 민감한 식물로 특히 NaCl 100mM에서 산화피해가 유의적으로 증가하는 것을 알 수 있었다.

A prostaglandin (PG), PGE2, mediates both cellular and humoral immune responses of insects. Spodoptera exigua (Lepidoptera: Noctuidae) biosynthesizes PGE2 by an unknown enzyme. This study predicted the enzyme, which is a novel membrane-bound prostaglandin E synthase-2 (mPGES-2). The transcriptional expression analysis showed the mPGES-2 is inducible to immune challenge. After RNA interference (RNAi), the hemocyte nodulation and expression of eleven antimicrobial peptides were significantly impaired. The RNAi effect was rescued by addition of PGE2 suggesting that mPGES-2 is crucial for immune responses mediated by PGE2.

감성은 복잡하고 다양한 요인들에 의해 영향을 받기 때문에 다각적인 측면에서 고려되어야 한다. 본 연구에서는 심리 평가 척도의 하나인 각성(arousal) 지표와 다중 생체신호에서 추출된 생체지표 반응을 이용하여 중립 및 부정감성(슬픔, 공포, 놀람)의 분류하였다. 이를 위하여 감성에 따른 생체지표 반응의 차이를 확인하였고, 다중 신경망 알고리즘 기반의 감성 인식기를 적용하여 이들 감성이 얼마나 정확하게 분류되는가를 확인하였다. 총 146명의 실험 참가자(평균 연령 20.1±4.0, 남성 41%)를 대상으로 감성 유발 자극을 제시하고 동시에 생체신호(심전도, 혈류맥파, 피부전기활동)를 측정하였다. 또한 감성 유발 자극에 대한 심리 반응을 감성 평가 척도로 평가하였다. 측정된 생체신호에서 심박률(HR), NN 간격의 표준편차(SDNN), 혈류량(BVP), 맥파전달시간(PTT), 피부전도수준(SCL), 피부전도반응 (SCR)을 추출하였다. 결과 분석을 위하여 감성 자극에 대한 각성도와 안정 상태와 감성 상태의 생체지표 반응을 활용하였다. 또한 감성 분류를 위하여 다중 신경망 기반의 감성 인식기를 활용하였다. 그 결과, 감성에 따른 생체지표 반응의 차이를 확인하였고, 이들 감성의 분류 성능은 각성도와 모든 생체지표 특징들을 조합하였을 때 정확도가 가장 높음(86.9%)을 확인하였다. 본 연구는 심리 및 생체지표 추출과 기계학습 기술의 적용을 통하여 부정 감성을 분류할 수 있음을 제안하며, 이는 인간의 감성을 탐지하는 감성 인식 기술을 확립하는데 기여할 것으로 예상한다.

Moringa oleifera leaves, seeds, pods, roots, and flowers have been widely used for their medicinal and nutritional properties. Many studies have been conducted on the chemical composition and effectiveness of M. oleifera. In fact, almost every part of M. oleifera has been found to contain essential nutrients and medicinal value. Especially, the leaves of M. oleifera are known to have various nutrients and diverse efficacy. Several studies have assessed the potential toxicity of the leaves when prepared by various methods. The results showed that the M. oleifera leaves when prepared differently were safe in locally used doses and amounts. Moreover, M. oleifera is known to contain various physiological efficacies, including antioxidant, anti-inflammatory, antidiabetic, and anticancer effects and so on. In the latest research, many attempts are being made to utilize the diverse effects of M. oleifera. This research seems to be bringing a promising view of M. oleifera as a therapeutic functional food for various diseases.

Shrimps infected with WSSV (White Spot Syndrome Virus) generally exhibit white spots in their inner space of carapaces as an acute clinical sign. In an effort to identify the correlation between this acute clinical sign and the condition, the index factors (RNA/DNA concentration and ratio, trypsin activity) were analyzed. A total 580 farmed Fenneropenaeus chinensis and 130 Lithopenaeus vannamei were collected from western and southern fifteen outdoor ponds in Korea. The status of the white spot pathology was divided into four stages (stage 0, stage I, stage II, and stage III), in accordance with the clinical signs as to the size and area of white spots. A significant decrease in RNA concentration and RNA/DNA ratio for multi-infected fleshy prawn (WSSV and vibrio sp.) occurred during the stage III (the whole carapace is covered with a white spot). In particular, RNA/DNA ratio was significantly lower as 1.47±0.04 than other groups. A similar trend was also found in the single infection (WSSV), but the decrease was less than the multi-infection. In the species comparison, both species were vulnerable to the multi-infection, but L. vannamei was more sensitive than F. chinensis (ANOVA, p<0.05): A significant decrease in RNA concentration and RNA/DNA ratio was first found in stage II for the former species, while it was found in stage III for the latter species. Trypsin activity was also showed a similar tendency with nucleic acid variation. Multi-infected shrimp showed drastically decrease of trypsin activity. According to the results, clinical signs of the white spot under carapace have an only physiological effect on shrimp if they covered entirely with white spots.

Physiological responses and activities of antioxidative enzymes were investigated in pepper(Capsicum annuum) seedlings subjected to low temperature stress. The seedlings were exposed to 7℃, 11℃, or 15℃ for 4 h during the early seedling stage of pepper plants. The results showed that plant height and chlorophyll content were unaffected by the treatments. Polyphenol content in seedlings exposed to 7℃ was higher than that at 11℃ and 15℃ for 2 h. After 3 h of treatment, the flavonoid content was higher in pepper seedlings exposed to 7℃ than 11℃ and 15℃, which was slightly higher than that detected in the control. The H2O2 content increased remarkably with increasing exposure time to low temperature(i.e., 7℃) as compared to that at 11℃ and 15℃. The levels of antioxidant enzymes superoxide dismutase(SOD), catalase(CAT), ascorbate peroxidase(APX), and peroxidase(POD) fluctuated. These results provide basic information that can be utilized to maintain efficient temperature in greenhouses for sustainable growth of pepper under severe low temperature conditions.

To enhance the physiological activity of the Rhynchosia volubilis (RV), R. volubilis (RVHE-A) and R. volubilis-added herbal powder (RVHE-B) were fermented with a solid state culture of Hericium erinaceum mycelia (HE). The total isoflavone contents of the non-fermented RV-A (489.9 μg/g) and RV-B (571.1 μg/g) were remarkably increased in fermented RVHE-A (1,836.4 μg/g) and RVHE-B (1,276.7 μg/g). In particular, aglycone isoflavones such as daidzein and genistein were significantly higher in the RVHE-A than any other sample. When hot-water (HW) and EtOH extracts (E) were fractionated from the RV and RVHE, both extracts from the RVHE-A were higher than those from the RV-A in total polyphenol and flavonoid contents. However, the RVHE-B-HW showed a lower polyphenol and flavonoid content level than did RV-B-HW. RVHE-A-HW and -E also had more potent ABTS radical scavenging activity than any extract from the non-fermented RV and other ferments (RVHE-B). In the meanwhile, RVHE-A-HW potently stimulated the production of macrophage activation-related cytokines such as TNF-α, IL-6 and IL-12 (841.7±71.3 pg/mL, 3.9±0.1 ng/mL, 179.3±30.2 pg/mL) from peritoneal macrophage more than RV-A-HW (92.5±1.5 pg/mL, 0.1±0.0 ng/mL, 37.4±5.4 pg/mL) as well as RVHE-B-HW (557.0±21.3 pg/mL, 1.8±0.0 ng/mL, 90.0±10.0 pg/mL). However, all the EtOH extracts did not show significant activity. In addition, the RVHE-A-HW showed a significantly higher intestinal immune system modulating activity through Peyer’s patch and GM-CSF production than did any other extract from RV and RVHE-B. In conclusion, these results suggest that the fermented R. volubilis with H. erinaceum mycelia possesses a possible use as an industrial application as functional food or material.

본 연구는 마치종 황색 알곡 품종으로 등록된 강일옥 옥수수 알곡의 처리별 시료 및 속대의 일반성분, 생리활성 검정, 색소 함량을 분석하여 기초자료로 제공하고자 수행되었다. 강일옥 옥수수를 알곡, 거피된 알곡, 알곡 껍질 및 속대로 분리하여 일반성분 분석 결과, 수분함량은 거피된 알곡이 12.04%로 가장 높았고 알곡 껍질의 조회분, 조지방, 조단백질 함량은 각각 3.19%, 8.59%, 12.01%로 강일옥 옥수수 처리별 시료 중 가장 높은 함량을 나타내었다. 강일옥 옥수수 처리별 시료의 무기성분 함량을 분석한 결과, 칼슘, 칼륨, 마그네슘, 철, 망간 및 인의 6종이 검출되었으며 공통적으로 검출된 주요 무기성분은 칼륨과 인이었다. 강일옥 옥수수 속대의 칼륨 함량이 114.33 mg/ 100 g으로 가장 높았으며 알곡 껍질의 인 함량이 2412.40 mg/100 g으로 가장 높게 측정되었다. 강일옥 옥수수 처리 별 시료의 항산화 활성 검정 결과, 강일옥 옥수수 속대 추 출물의 DPPH radical 소거능은 93.05%, ABTS radical 소 거능은 79.88%로 처리별 시료 중 가장 높게 측정되었으며 알곡, 거피된 알곡 및 알곡껍질 추출물의 DPPH 소거 능은 모두 70% 이상으로 우수한 항산화활성을 나타내었다. 강일옥 옥수수 처리별 시료의 항염 활성 검정 결과, 강일옥 옥수수 처리별 시료 추출물은 Raw264.7 대식세포 의 생존율에 영향을 미치지 않은 것으로 나타났으며 NO 생성량은 모든 시료 처리군에서 LPS 단독 처리군(con(+)) 에 비하여 유의적으로 NO 생성을 억제 하는 것으로 나타났다. 강일옥 옥수수 처리별 건조분말시료의 카로티노이드 색소 함량을 분석한 결과, 모든 시료에서 β-carotene은 검출되지 않았고, lutein과 zeaxantin은 알곡과 알곡 껍질 에서 검출되었으며 거피된 알곡과 속대에서는 검출되지 않았다. 강일옥 옥수수 알곡의 lutein과 zeaxantin의 함량은 각각 115.30 μg/100 g, 50.92 μg/100 g이었으며 알곡껍질의 lutein과 zeaxantin의 함량은 각각 37.98 μg/100 g, 19.27 μg/100 g이었다.

본 연구에서 국내에서 개발된 제초제저항성 GM 벼인 밀양 204호와 익산 483호가 비표적 곤충과 거미에 미치는 영향을 평가하였다. GM 벼와 non-GM 벼에 대한 보리수염진딧물과 벼멸구의 기주선호도에는 차이가 없었으며, GM 벼를 섭식한 벼멸구를 포식한 황산적거미의 체중 변화에도 통계적 유의성이 없었다. 밀양 204호와 그 대조 모본 non-GM 벼에서 서식한 벼멸구를 포식한 황산적거미의 생존율에는 차이가 없었 으나, 벼멸구를 통해 익산 483호에 노출된 황산적거미의 생존율은 대조 모본 non-GM 벼에 비해 통계적으로 낮은 생존율을 나타내었다. 또한 익 산 483호의 화분을 섭식한 꿀벌 유충은 밀양 204호와 다른 non-GM 벼 화분을 제공하였을 때 보다 현저하게 긴 용기간을 보였다. 농업생태계에 서 중요한 포식 천적인 황산적거미와 양봉 산업으로서 중요한 기능을 하는 꿀벌에 GM 벼가 부정적인 영향을 보임에 따라 국내에서 개발된 제초 제저항성 GM 벼의 농업 환경 노출 이전에 충분한 추가 연구와 안전성 평가가 선행되어야 할 것으로 사료된다.

Plant physiological indices for Euryale ferox Salisb. an endangered aquatic plant from Gyeongsan Province, were analyzed in this study. The NDVI (Normalized Difference Vegetation Index) was highest at 0.820 in Dongguji and lowest at 0.592 in Dongjaji; the SR (Simple Ratio) index was highest at 10.240 in Dongguji and lowest at 4.312 in Dongjaji; and the PRI (Photochemical Reflectance Index) was highest at 0.039 in Dongguji and lowest at −0.036 in Dongjaji. The CRI1 (Carotenoid Reflectance Index 1) index was in Sahwaji and Waji at 8.881 and 12.988, respectively, and the CRI2 (Carotenoid Reflectance Index 2) index was highest at 7.889 in Sahwaji and lowest at 11.889 in Dongjaji.

We studied growth and physiological responses of Ambrosia trifida L. under the different light condition. Ambrosia trifida L. is Korean invasive species. For study, we selected the two spot (T1 and T2) in the Kongju National University. In each spots, we investigated growth and physiological responses in the more light environment (L) and in the less light environment (S). it makes 4 research spot (T1-L, T1-S, T2-L and T2-S). and first, we estimate light intensity (Lux) in each research spots. then take data on the growth responses which are a shoot height, a number of leaves, a leaf width and a leaf length. Also, we get some physiological data which are a photosynthetic rate, a transpiration rate, a stomata conductance, water use efficiency, chlorophyll content, photochemical efficiency of photosystem II (Fv/Fm). And, analysed them by using one-way ANOVA. As a results, the shoot height, No. of leaves, Leaf width and Leaf length were recorded relatively high in the spot of the most light quantity and in the spot of the least light quantity. In the physiological responses, the more light quantity was increased, the more photosynthetic rate, transpiration rate and stomatal conductance were increased. Fv/Fm, the photochemical efficiency of photosystem II, was the least recorded in the spot of the most light quantity. These results show Ambrosia trifida grow well in the spot of the most and the least light quantity but it grown in the shade has the less environmental stress.l

There are three kinds of Pleurotus eryngii : P. eryngii var. eryngii (P. eryngii), P. eryngii var. ferula (P. ferula : A-Wei-Mo) P. eryngii var. nebrodensis (P. nebrodensis : Bai-Ling-Gu). We bred P. ferula using Di-Mon mating and physiological assay and selected as follow (1) ‘Beesan No.1’ produced high yields, (2) ‘Beesan No.2’ was excellent morphological shape and anti-adipogenic Activit, (3) ‘Ergo’ included high ergothioneine such as anto-oxidant material, (4) ‘GW10-45’ included highest ergothioneine such as anto-oxidant material, (5) ‘Maeksong’ included high ergothioneine and effect of anti-inflammatory. We suggest that there are able to food-medicine materials.