This study was carried out to select optimal shade materials among four-layered polyethylene (PE) net (FLPN), aluminium-coated PE sheet (APSS), and blue PE sheet (BPSS) in condition of paddy field cultivated 6-year-old ginseng. The order of light-penetrated ratio and air temperature by shade materials was BPSS 〉 APSS 〉 FLPN. Light-penetrated ratio of BPSS before two fold shade was more 3 times and 2 times than that of FLPN and APSS, respectively. Air temperature of BPSS was also higher 1.6℃ and 1.4℃ than that of FLPN and APSS, respectively. BPSS showed good cultural environment because all of light-penetrated ratio and air temperature were become higher in spring and fall season but lower in summer season by additional shade with two-layered PE net. Survived-leaf ratio was highest in BPSS and lowest in FLPN causing a little water leak on a rainy day. Rusty-root ratio was also highest in FLPN because soil moisture content was increased by water leak. The order of root yield was BPSS 〉 APSS 〉 FLPN, and the cause of highest yield in BPSS was higher light-penetrated ratio during spring and fall season, higher survived-leaf ratio, and lower rusty-root ratio than that of APSS and FLPN. BPSS showed highest total ginsenoside content because of high light-penetrated ratio, blue light effect, and the difference in dry matter partitioning ratio such as low taproot ratio, and high lateral root ratio.

본 연구는 인삼 기내에서 생산된 재분화 식물체의 토양 순화를 위해 순화과정을 단축하고 생존율을 높일 수 있는 방법을 개발하기 위하여 실시하였다. GA3를 0.4mg l-1로 9시간을 처리하였을 때 토양에서 생존율은 59.6%로 가장 높게 나타났으며, 그 다음이 1mg l-1에서 9시간을 처리하였 때 43.7%의 생존율을 나타냈다. 재분화 식물체의 뿌리길이가 4 cm 이상일 때는 48개체가 생존하여 생존율은 53.3%로 처리 중 가장 높게 나타났으며, 뿌리 무게는 4 g 이상일때는 46.7%가 생존하여 가장 높은 비율을 나타냈다. 4년생 때 지상부 생육 특성을 조사한 결과, 초장은 35.3 cm, 경장은 18.3 cm, 엽장은 12.1 cm, 엽폭은 4.8 cm로 이식 재배한 연풍의 생육 특성과 비교해 보면 재분화 식물체의 생육이 약간 떨어짐을 볼 수 있었다. 3년생 때 지하부 특성을 보면 근장은 15.3 cm, 동장은 4.5 cm, 동직경은 2.1 cm, 그리고 근중은 15.4 g으로 지하부도 지상부와 마찬가지로 대조구인 묘삼을 이식하여 재배한 처리구에 비하여 생육이 약간 떨어졌다.

This study was carried out to know the substitute effect of Yacto (leaf mold compost) on popped rice hulls compost (PRHC) in Yang-jik nursery bed of Panax ginseng. PRHC was mixed with Yacto as 50:50 ratio, and 1 ~ 2% of the mixed oil cake, rice bran and urea were also added to promote decaying the mixed compost. The mixed compost made by PRHC and Yacto was showed that positive effect on the growth of ginseng seedling when it was mixed with 1% of oil cake and rice bran, and 2% of mixed oil cake. But addition to the 2% of urea in the mixture of PRHC and Yacto was not positive effect on the growth of ginseng seedling. Root yield of the mixed compost was similar to that of conventional compost by made 100% of Yacto. Therefore, the mixed compost can substitute for Yacto when PRHC and Yacto were mixed by 50:50 ratio and added 1% of oil cake and rice bran.

This study was conducted to select plant growth regulators effective at ginseng berry set inhibition to help root growth in Korean ginseng (Panax ginseng C. A. Meyer). PGRs (ethephon, gibberellic acid, maleic hydrazide, coumarin) were applied to field grown 5-year-old Korean ginseng between one and two times, before and during bloom in 2009, 2010. The number of treatment was more effective in ginseng berry set inhibition when used two times compared with one time in GA 1,000 ppm, MH (5,000, 10,000 ppm), coumarin (5,000, 10,000 ppm) treatment. According to treatment period of plant growth regulator, ginseng berry set inhibition rate from 20days before flowering date to 5days after blooming was the highest in MH 5000 ppm showing 99.9% and the lowest in GA 100 ppm showing 32.8%. The spray treatments of Ethephon (50, 150 ppm) and MH (5,000, 10,000 ppm) from 20 days before the flowering bloom up to 5 days before, and coumarin (5,000, 10,000 ppm) from 20 days to 6 days and before blooming that induced the inhibitory effect more than 90% after 12 weeks. Considering ginseng berry set inhibition characteristics and treatment period ethephon and coumarin was important about applied period but, MH treatment appeared to effective ginseng berry set inhibition regardless of treatment period.

This study was performed to enhance contents of low molecular weight ginsenoside Rh2 and Rg3 using an ultra high pressure and steaming process in wild cultured-Root in wild ginseng. For selective increase in contents of Rg3 and Rh2 in cultured wild ginseng roots, an ultra high extraction was applied at 500MPa for 20 min which was followed by steaming process at 90℃ for 12 hr. It was revealed that contents of ginsenosides, Rb1, Rb2, Rc and Rd, were decreased with the complex process described above, whereas contents of ginsenoside Rh2 and Rg3 were increased up to 4.918 mg/g and 6.115 mg/g, respectively. In addition, concentration of benzo[α]pyrene in extracts of the cultured wild ginseng roots treated by the complex process was 0.64 ppm but it was 0.78 ppm when it was treated with the steaming process. From the results, it was strongly suggested that low molecular weight ginsenosides, Rh2 and Rg3, are converted from Rb1, Rb2, Rc, and Rd which are easily broken down by an ultra high pressure and steaming process. This results indicate that an ultra high pressure and steaming process can selectively increase in contents of Rg3 and Rh2 in cultured wild ginseng roots and this process might enhance the utilization and values of cultured wild ginseng roots.

The management and control of mineral nutrients is one of most important techniques to increase the productivity and the quality of Korean ginseng. The mineral nutrients are measured with different plant tissues and different growth stages of 2-year-old ginseng grown under hydroponic culture with two different temperatures. The content of N, P, Ca, and Mg were higher at low temperature in both leaves and roots than those at high temperature. However, the content of K was high in leaves at low temperature compared to that of high temperature, while it was not significantly different in roots. The uptake amounts of N and K was higher throughout the experimental period at low temperature in both leaves and roots than those at high temperature. However, the uptake amount of P was not clearly different between two different temperatures and among six different growth stages. The uptake amount of N, P, K was generally decreased in leaves from June to August, while it was increased in roots. The relationship between dry weight and mineral nutrients in leaves was appeared positive with N, K, Ca, and Mg, but negative P. In roots, N, K, Ca, and Mg were negative, showing that was positive with only P. Comparing the correlation coefficients among mineral nutrients in leaves, N and K were significantly positive correlation each other. P was significantly positive correlation with Na and Zn. In case of roots, N was highly significant positive correlation with K, Mg, and Mn, but P was negatively correlated with Ca, Cu, Na, Fe, and Zn.

The management and the use of major mineral nutriments such as nitrogen, phosphorous, and potassium, etc have been practiced and improved in various cultivating methods of Panax ginseng C. A. Meyer. The purposes of this study were to examine the content of major mineral nutrients on different ginseng aging from 1 to 6 years old, to analyze their uptake and utilization in tissues of ginseng, and to find out their proper managing techniques throughout the cultivation of ginseng. In case of the leaves, the N content was not clearly different from 1 to 6 years old, while the content of P and K was generally decreased throughout the cultivating years. In case of the roots, the content of N and K was gradually decreased from 1 to 6 years old, while the P content was increased until 3 years old, decreased at 4 years old, increased again at 5 years old, and decreased again at 6 years old. The uptake amount of N was increased in root of ginseng from 1 to 6 years old, 0.02 to 2.79kg/10a based on dry weight, respectively. Other minerals of P, K, Ca, and Mg were increased for the cultivating year. Comparing the uptake amounts of N, P, K with different cultivating year, they were the highest uptake amount at 4 years old and then were decreased. The management techniques of major mineral in cultivation of ginseng would be studied and evaluated more in order to have better ginseng production.

This study was carried out to have the basic and applied informations relating to increase the productivity and quality of ginseng. 2 years-old ginseng was cultivated under hydroponic culture with the controlled environment conditions in a greenhouse. Major growth characters and yields were investigated with two different temperatures and several growth stages. The plant height and stem diameter were higher at low temperature than those at high temperature. They were not clearly different with six different growth stages. The root length was not clearly different between two temperatures; however it was continuously grown from June until August. The root diameter was higher at low temperature than that at high temperature. It was rapidly increased from June until August. The length, width, and area of leaf were higher at low temperature than those at high temperature. The fresh and dry weights of different plant tissues were also heavier at low temperature than those at high temperature. The moisture content of ginseng root was continuously decreased from June until August. The yield of ginseng was higher at low temperature compared to that at high temperature. The cultivating conditions in hydroponic culture of ginseng, especially temperature, would be an important factor to have better growth and production.

To develop the practical cultivation for paddy field, we investigated the properties of paddy soil, growth characteristics and ginsenoside content of 6-year-old ginseng, Cheonpung variety between poor drainage class (PDC) and imperfect drainage class (IDC). Groundwater level in PDC showed monthly small changes of 20~30 cm, while IDC showed monthly great changes of 28~71 cm depending on rainfall. Soil moisture content in PDC and IDC was 17.2%, 22.5%, respectively. Air temperature in IDC was lower than 0.3℃, while soil temperature was higher than 0.8℃ compare to PDC, respectively. Main soil color of PDC was grayish olive, while IDC was brownish olive. PDC showed yellowish mottles only at underground of 20~40 cm, while IDC showed that at underground of 30~90 cm. IDC showed lower pH, EC, potassium, calcium and magnesium content, but higher organic matter, phosphate, and iron content than that of PDC, respectively. All of EC, organic matter, potassium, calcium, and magnesium content were decreased, but iron content was increased at the subsoil layers of PDC. All of EC, organic matter, phosphorus, and potassium content were decreased, but calcium and magnesium content were increased at the subsoil layers of IDC. Root yield in IDC was more increased by 33% than that of PDC. The moisture content and rusty ratio of ginseng root in IDC were lower than that of PDC. Ginsenoside content in IDC was higher than that of PDC because the ratio of lateral and fine root showing relatively high content of ginsenoside was higher in IDC than that of PDC.

본 연구는 CO2 농도와 온도가 상승함에 따라 인삼의 생육반응 및 광합성 특성의 변화를 알아보기 위해 실험하였다. 유리 온실 안에 대조구(대기중 CO2 농도)와 CO2 + 온도상승구(750-800 ppm, 2℃ 상승)로 나누어 비교하였으며 다음과 같다. 1. 인삼의 개엽율은 1년생 인삼을 이식한 후 1주일 이후인 4월 6일에 CO2 + 온도상승구의 개엽율이 대조구보다 더 높았다. 그러나 최종 개엽율은 CO2 + 온도상승구간 차이가 없었다. 2. 꽃이 나오는 시기는 대조구보다 CO2 + 온도상승구가 3일 빨랐고, 열매관찰시기와 열매 성숙시기는 대조구와 CO2 + 온도상승구간 차이가 없었다. 3. 인삼의 줄기길이는 대조구보다 CO2 + 온도상승구에서 길었고, 잎수는 대조구보다 CO2 + 온도상승구에서 많았다. 4. 지하부 생량무게는 대조구와 CO2 + 온도상승구간 차이가 없었다. 5. 광합성은 모두 대조구보다 CO2 + 온도상승구가 높았다. 대조구 내에서는 년도별 광합성이 차이가 있었다. 하지만 CO2 + 온도상승구에는 1년생과 2년생 간 차이가 없었다. 6. 증산률은 대조구와 CO2 + 온도상승구에서 모두 년생간 차이가 있었다. 1년생은 대조구보다 CO2 + 온도상승구가 높았고, 2년생은 대조구와 CO2 + 온도상승구간 차이가 없었다. 7. 수분이용효율은 대조구와 CO2 + 온도상승구에서 모두 1년생과 2년생간 차이가 있었다. 1년생은 대조구와 CO2 + 온도상승구간 차이가 없었고, 2년생은 대조구 보다 CO2 + 온도상승구가 높았다. 이상으로 볼 때 지구온난화는 인삼의 생육과 생리 생태학적 반응에 다소 긍정적인 영향을 준다.

An important factor in the production of organic ginseng is the control of Alternaria blight and anthracnose, which mostly affect the leaves in the summer. We compared the effects of a lime-bordeaux mixture (LBM) and agricultural chemicals on the growth characteristics and ginsenoside content in 4-year-old ginseng plants when they were sprayed at 15-day intervals from mid-June to the end of September. The increases in leaf length, and survive-leaf ratio in plants sprayed with LBM were greater than the increases of the control plants, but less than those of agricultural chemicals treatment. The root weight per plant in the plants sprayed with LBM increased more distinctly than that in the control plants, while it was significantly lower than that in plants sprayed with agricultural chemicals. The root yield in plants sprayed with LBM increased by 21% compared to the root yield in the control plants, but decreased by 7% compared to that in plants sprayed with agricultural chemicals because of the decreases in leaf area and survive-leaf ratio. Spraying of LBM had a significant effect on the ginsenoside contents. The total ginsenoside content was highest in the control plants and lowest in the plants sprayed with agricultural chemicals and total ginsenoside contents was great relative to survive-leaf ratio and root weight.

The present study examined the effects of Korean white ginseng (WG, Panax ginseng C. A. Meyer) on the learning and memory function and the neural activity in rats with trimethyltin (TMT)-induced memory deficits. The rats were administered with saline or WG (WG 100 or 300 mg/kg, p.o.) daily for 21 days. The cognitive improving efficacy of WG on the amnesic rats, which was induced by TMT, was investigated by assessing the Morris water maze test and by performing immunohistochemistries on choline acetyltransferase (ChAT), acetylcholinesterase (AchE), cAMP responsive element binding protein (CREB) and brain derived neurotrophic factor (BDNF). The rats treated with TMT injection (control group) showed impaired learning and memory of the tasks, but the rats treated with TMT injection and WG administration produced significant improvement of the escape latency to find the platform in the Morris water maze at the 2nd and 4th days compared to that of the control group. In the retention test, the WG 100 and WG 300 groups showed significantly increased crossing number around the platform compared to that of the control group (p < 0.001). Consistently with the behavioral data, result of immunohistochemistry analysis showed that WG 100 mg/kg significantly alleviated the loss of BDNF-ir neurons in the hippocampus compared to that of the control group (p < 0.01). Also, treatment with WG has a trend to be increased the cholinergic neurons in the hippocampal CA1 and CA3 areas as compared to that of the control group. These results suggest that WG may be useful for improving the cognitive function via regulation of neurotrophic activity.

병결점 부근의 저장온도에 따른 수삼의 내적 품질 변화 연구로 수삼을 각각 , 및 에서 16주간 저장하면서 저장온도에 따른 호흡률, 경도, 가용성 고형물함량, 전분함량, pH, 사포닌함량과 관능적 품질의 변화를 조사하였다. 수삼의 호흡률은 저장온도가 낮을수록 낮았으나 그 차이는 미미하였다. 수삼 표피층의 정도는 저장기간이 경과됨에 따라 증가하였고, 내부 조직의 경도는 감소하는 경향을 보였으나 저장온도에 따라 경도 변화의 차이는 뚜렷하지 않았다.

The principal objective of this study was to develop a discrimination method using SSR markers in Korean ginseng cultivars. Five cultivars--Chunpoong, Yunpoong, Gopoong, Sunpoong, and Kumpoong--were evaluated by nine markers out of 22 SSR markers. A total of 23 alleles were detected, ranging from 1 to 4, with an average of 2.6 alleles per locus, and an averages of gene diversity (GD) of 0.480. Nine markers were tested in order to distinguish among five Korean ginseng cultivars. Two markers out of nine SSR markers, GB-PG-065 and GB-PG-142, were selected as key markers for discrimination among Korean ginseng cultivars. Two genotypes were detected in GB-PG-065. Chunpoong and Kumpoong shared the same allele type, and Yunpoong, Gopoong, and Sunpoong shared another identical allele type. In the case of GB-PG-142, a specific allele type differentiated from those of other four cultivars was observed only in Sunpoong cultivar. Consequently, the SSR markers developed in this study may prove useful for the identification of Korean ginseng cultivars and the development of ginseng seed management systems, as well as tests to guarantee the purity of ginseng seeds.

Growth characteristics, root yield and ginsenoside contents of 3-year-old ginseng in greenhouse shaded by 30˚ sloped-curtain made of aluminum were compared to traditional shade facility in order to develop cultural practice for organic ginseng. Light transmittance ratio in greenhouse with 30˚ sloped-curtain shade was distinctly lower than that of traditional shade from sunrise to 9 a.m., while its ratio in greenhouse was higher than traditional shade since 9 a.m. due to the reflection of light. Air temperature of greenhouse was 1.3℃ higher than that of traditional shade on the first ten days of August due to more reflected light. Root yield of greenhouse was 44% higher than that of traditional cultivation because of the inflow of reflected light and the decrease of disease of Alternaria and Anthracnose by blocking rainfall. Dry matter partitioning ratio of rhizome and lateral root were increased in ginseng cultivated at greenhouse due to longer survival time in leaf than traditional cultivation. Total ginsenoside contents cultivated at greenhouse was decreased in the part of taproot, while it was increased in the part of lateral and fine root compare to traditional cultivation. Individual ginsenoside contents between greenhouse and traditional cultivation showed significant difference more frequent in fine root than taproot and lateral root. Total ginsenoside contents including Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, and Rg2 in whole root of 3-year-old ginseng did not showed significant difference by greenhouse and traditional cultivation.

This study was conducted to examine major growth responses, the production and partitioning of dry matter on different growth stages, and yields and to select the optimal shading material in both quality and productivity of ginseng. Two cultivars of ginseng, Cheonpoong and Geumpoong, were cultivated in the paddy soil with three different shading materials; three-layered blue and one-layered black polyethylene (TBP), blue polyethylene sheet (BPS), and aluminium-coated polyethylene sheet (APS). Plant heights were linearly increased until June 24 and then maintained with showing higher height in Cheonpoong than that in Geumpoong cultivar. Root lengths were gradually increased until October 16. They were longer in Cheonpoong than that in Geumpoong cultivar, showing slightly longer with APS compared to TBP and BPS. The ability of producing dry matter of leaves was much higher from April to June compared to those of other growth periods, whereas its ability of root was concentrated from the end of June to the end of August. Among the shading materials, the ability of producing dry matter of shoot was higher with TBP than those with BPS and APS, while its ability of root was not appeared certain tendency unlike the shoot. The yield of ginseng roots was the highest with TBP among three shading materials and it was higher in Cheonpoong than that of Geumpoong cultivar. The shading materials which affect the light intensity and the temperature would be considered as an important factor to get better quality and productivity of Korean ginseng.

국내에서 수집된 인삼 자원 중에서 작물학적 특성이 우수하다고 조사된 54점(6~7년 근)을 대상으로 UPOV 조사 기준에 따라 주요 형태적인 특성들을 조사한 결과 경수는 1~4개 범위로 1개인 것이 42.6%이고, 2개인 것이 38.6%로 경수가 1~2개인 것이 전체 81.2%로 나타났으며, 경수가 5개인 것은 없었다. 장엽수는 3~6엽 범위로 5엽이 55.6%였고 4엽인 것은 25.9%였다. 경색은 자색계열이 전체 81.5%로 자색이 46.3%, 연자