Background : Ginseng seeds are one of short-lived seeds species which loose their viability easily in the condition of conventional storage. Cryopreservation using liquid nitrogen (LN) has been recommended as a alternative storage for this kind of germplasm short lived or dessiccation-sensitive. This study was performed to find out whether cryopreservation could assure initial viability not only for seeds with high germination rate but also for seeds with low germination rate (in aging process). Methods and Results : In this work, 3 cultivars of dehisced ginseng seeds were artificial aged in the condition of 40℃, 95% RH during 6 hours with 3 hours-interval. The germination rate of ginseng seeds was decreased to the range of 71 - 94% of initial viability by artificial aging treatment. After 24 hours of vapor-LN exposure on both of artificial aged and non treated seeds as a cryopreservation, germination rate for each cultivar was decreased with the range of 76 - 95% of initial viability. While the decreasing patterns of germination rate for each cultivar showed similar curves between before and after vapor-LN exposure, the aging effects could be slightly little by cryopreservation for 3 cultivars of ginseng seeds. Conclusion : From the above results, we may suggest that cryopreservation could be recommened for storage tool of dehisced ginseng seeds even with low viability also and expected to make slower seed aging process during preservation period through further study.

Background : Alpha-linolenic acid is a type of omega-3 fatty acid and has been reported to be found at a remarkably high content in seeds of perilla (Perilla frutescens). The purpose of this study was to investigate the fatty acid compositions in 45 perilla accessions collected from Russia and recommend the potential genetic resources related to their fatty acid compositions. Methods and Results : The 45 accessions of perilla seeds which were collected from Russia were used for the study. Perilla seed oil was recovered using hexane in a soxhlet extraction method. The fatty acid compositions were analyzed using gas chromatography. The total oil content was ranged between 28.39 and 46.89%. The compositions of palmitic, stearic, oleic, linoleic and linolenic acid ranged from 5.57 to 7.09%, 1.16 to 2.27%, 11.83 to 19.55%, 11.92 to 16.71%, and 59.19 to 67.28%, respectively. Cultivars 'Dayu', 'Daeyu' and 'Anyu' showed lower linolenic acid composition compared to the average value of linoleic acid in perilla germplasms collected from Russia. Cluster analysis based on the fatty acid composition of the 45 perilla accessions segregated into three groups. Group Ⅰ characterized as higher palmitic, stearic and oleic acid compositions compared to other groups. Group Ⅱ which contained 12 accessions had high total oil and linoleic acid composition. Group Ⅲ characterized as a higher linolenic acid composition compared to other groups (p < 0.001). Oleic acid showed a significant negative correlation (r = -0.825) with linolenic acid. Principal component analysis (PCA) revealed that the first two principal components together explained 74.4% total variation. Conclusion : Our results indicated that accessions IT235818, IT235820, IT226739 which exhibited high linolenic acid composition could be useful to develop new functional vegetable oil materials.

Background : Polygala tenuifolia is mainly distributed in Korea and China. It is known to be mainly helpful for brain function and has been used medicinally for a long time. Currently, most of that used in domestic are imported from China. We want to identify the basic characteristics to replace imports and grow into new domestic income crops. Previous studies have examined the storage conditions of seeds and the areas suitable for cultivation. In this study, seed harvesting, germination conditions and seedling production methods are explained. Methods and Results : Seed harvest method - in early June, we have covered the funnel, paper bag and mesh bag. Seed harvested at the end of June were sorted by wind speed of 3.5 m/s – 6.1 m/s and 1 ㎜ - 4 ㎜ mesh size. The germination rate was measured at 20℃ for 10 days. Suit of the germination temperature - the germination rate was measured by 100 seeds, three replicates with a temperature gradient of 15℃ to 35℃ and 2℃ intervals. In order to confirm the conditions for seedling production, the emergence rate was examined at 20℃, 25℃, 30℃ and 35℃ in growth chamber. The seed harvesting method was 1.88 g of mesh bag the highest yield. The best sorting method was 2 ㎜ mesh size and 5.7 m/s wind speed. The germination temperature was the highest at 89.7 - 90.3% at the temperature range of 1 9℃ - 20℃ and the germination rate was decreased to 21.0% at 27℃. The seedling production rate was highest at 20℃. There was no difference in growth according to the pot size, but the survival rate was the highest in 105 size Conclusion : As a result of this study, Polygala tenuifolia was confirmed that harvesting by mesh bag and sorting by size and wind speed were effective for seed quality and yield. The seed germination was observed at relatively low temperature. These results can be used as basic data for cultivation.

The aim of the study was to confirm whether the coriander seeds ethanol extract (CSEE) exhibited effective antioxidant activity and oxidative stability in corn oil. The results showed that the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) cation radical scavenging activity were 24.4, 55.0, and 81.0, and 8.9, 16.8, and 34.3% at the concentrations of 0.25, 0.5, and 1.0 mg/mL, respectively. The ferric reducing antioxidant power (FRAP) reduction power was 284.1 μM ascorbic acid equivalent/g extract, and the total phenol content (TPC) was 31.9 μM tannic acid equivalent/g extract. Furthermore, the TPC showed positive correlations with the DPPH radical scavenging activity, ABTS cation radical scavenging, and FRAP value (p<0.01). oxygen radical absorbance by fluorescein (ORAC) analysis showed that the antioxidant activities of trolox 50 μM and CSEE 100 μg/mL were 3.1 and 4.4 times higher than those of blank AUC, respectively. In addition, CSEE reduced the amounts of conjugated diene and ρ-anisidine by 8.3 and 40.8%, respectively, in the oxidized corn oil. Thus, the coriander seeds ethanol extract is confirmed to have effective antioxidant activity and oxidative stability in corn oil, and it can be used as a natural antioxidant for preservation in food processing.

Backgoound : Sprout vegetables are rich source of nutrients for human health. The safflower seeds contained various functional compounds and nutritions, and can be used as food material without restriction. Therefore, this study was performed to compare the antioxidant properties of sprouts from safflower seeds according to germination periods. Methods and Results : The safflower seeds were sterilized and steeped with distilled water, then incubated at 25℃ for 1 - 7 days. These sprouts and seeds were extracted with methanol, and used as samples for antioxidant properties (total phenolics, total flavonoids contents, DPPH, and ABTS radical scavenging activities). Total phenolics, total flavonoids contents, and antioxidant activities were higher in safflower seed than sprouts. Among sprouts with various germination periods, the sprouts incubated 4 - 7 days were higher in total phenolics, total flavonoids contents, and antioxidant activities than other sprouts. The levels of antioxidant activities were similar in sprouts incubated for 4 - 7 days. Conclusion : These results indicated that the antioxidant properties of sprouts differed according to germination periods, and the optimal germination period of safflower seed as sprout vegetables are 4 days.

Background : Perilla (Perilla frutescens) seed oil is known to contain high omega-3 fatty acid than other plant oils. This study was aimed to investigate the fatty acid composition in seeds of 255 perilla accessions from South Korea and recommend the potential genetic resources rel ated to their fatty acid composition. Method and Results : Total oil was extracted by soxhlet extraction apparatus and the fatty acid compositions were analyzed by gas chromatography (GCMS QP2010 ULTRA, SHIMADZU, JP). Total oil contents ranged from 22.41 and 47.62% with an average content of 34.20%. Palmitic, stearic, oleic, linoleic, and linolenic acid contributed 4.46 to 7.67%, 1.64 to 4.11%, 9.15 to 26.39%, 11.89 to 28.76%, and 50.24 to 64.16% to the total oil content, respectively. Seeds from Jeollabuk-do showed the highest average total oil content (41.14%) compared to other regions (p < 0.05), while the linolenic acid composition was the highest in the samples from Chungcheongnam-do (59.48%) (p < 0.05). The cluster analysis segregated the perilla seed accessions into two major clusters. ANOVA revealed that there was a significant difference between group Ⅰ and group Ⅱ (p < 0.05). Group Ⅰ (86 accessions) characterized as higher palmitic, stearic, oleic, and linolenic acid compositions than group Ⅱ (169 accessions). The highest content of linolenic acid were recorded in accessions K126190, K135903, and IT283646 from group Ⅰ, and accessions IT108680, IT208894, and IT111050 from group Ⅱ had high total oil content. Linoleic acid content showed a strong negative correlation with palmitic acid (r = -0.726*) and oleic acid (r = -0.678*) content. Principal component analysis (PCA) revealed that the first two principal components together explained 68.75% total variation. Conclusion : Our results showed that accessions K126190, K135903, IT283646 which exhibited high linolenic acid composition and accessions IT108680, IT208894, IT111050 which exhibited high total oil composition, could be useful to develop new functional oil materials.

Background : Licorice (Glycyrrhiza uralensis F.) is one of the most widely used raw materials for food and pharmaceuticals, and is currently the No. 1 imported medicinal plant in Korea. Efforts to settle licorice cultivation in Korea have been carried out for hundreds of years but have not succeeded, and many people suspect that the reason is due to the inadequate cultivation environment. However, even until recently, most licorice has been procured by wild harvesting, so studies on licorice cultivation technique and cultivation environment have not been conducted much in the world. This experiment was conducted to investigate the germination characteristics of licorice by temperature and salt concentration in order to find the optimal cultivation environment conditions of licorice. Methods and Results : The germination rate of licorice seeds was high 77.5 - 90.5% at 10 - 3 6℃, but it started to drop from 66.5 - 68.0% at 38 - 40℃ and it decreased sharply from 9.0 - 15.0% at 42 - 46℃. No longer germinated at temperatures above 48℃. The average number of days of germination was 12.3 days at 10℃, 5.7 days at 20℃, 3.5 days at 30℃ and 4.1 days at 40℃. The germination start date was 2 to 4 days in the 18℃ - 46℃ range. The average germination rate was the highest at 32℃. The germination time of 40% was 2 - 4 days at 18 - 46℃. The germination of more than 80% took 3 - 5 days at 20 - 40℃, 6 - 8 days at 14 - 1 8℃ and 10 days at 10 - 12℃. Licorice was able to germinate even in the presence of salt. Seed germination rate was 78.5 - 85.5% at 0 - 1% salt concentration and 63% at 1.5% and 18% at 2.0%. In addition, 8.5% seeds germinated successfully even at 3.0% salt concentration. Conclusion : Licorice has been known as a low temperature crop, but germination was possible in a wide range of temperature and salt concentration. Especially, the germination characteristic was higher in the high temperature range of 30 - 36℃, which corresponds to the domestic summer temperature. Therefore, the results of this study are expected to be useful reference for exploring suitable cultivation area in Korea.

The aim of this study was to determine the suitability of surfactant to extract higher phenolic compound, flavonoid and antioxidant activity from Tartary buckwheat and evaluate the potentiality of surfactant as a screening agent for breeding purpose. Primarily, we employed two types of surfactant (Hydrophilic: Tween 20 and Lipophilic: Span 80) to select the suitable surfactant agent for the extraction of optimum bioactive compounds. Between two surfactants, Tween 20 showed highest efficiency at 4 mM concentration to extract total phenolic content (TP), total flavonoid (TF) and antioxidant activity (AA). Tween 20 at 4 mM concentration was fixed for further analysis along with hot water (90℃) treatment as a control. In our findings, highest TP (118 ㎎/g), TF (38 ㎎/g) and AA (76%) was achieved in KW21 and KW22 among the fifteen accessions of Tartary buckwheat. In other way, TP, TF and AA was 200%, 120% and 110% higher in surfactant formulation compared with control treatment, respectively.

Persimmon seeds contain considerable amounts of minerals, amino and organic acids, natural antioxidants and phenolic compounds. The objective of this study was to investigate quality characteristics and antioxidant potential of Korean persimmon seeds. The pH (4.88-4.94), color values, contents of minerals, free amino acids, organic acids, and phenolic compounds and DPPH free radical scavenging potentials of persimmon seed extracts significantly (p < 0.05) varied with the genotypes. This study showed that the seeds could be used as a source of different mineral elements (47.14-85.07 ㎎/㎏) without any measureable amount of heavy metals such as arsenic, cadmium, lead and mercury. Similarly, considerable amounts of organic (1550.13-2413.08 ㎎/㎏) and essential amino (50.85-54.03 ㎎/㎏) acids and total phenolic compounds (1227.91-1307.78 ㎍ gallic acid equivalent/g) were also found in the seed extracts, indicating their potential food value as a natural antioxidant. Results of the present study imply that prethanol-A, a food preservative, can be used as an effective extraction to obtain the minerals, organic and free amino acids, and phenolic compounds from the persimmon seeds, which possess a big potential to be commercially used in food, cosmetic and pharmaceutical industries.

본 연구는 고 에너지 X-선을 조사한 새싹식물 종자를 파종 및 재배하여 산도, 염도, 당도, 당산비의 변화 를 분석하고 식물의 생장에 미치는 영향을 알아보고자 하였다. 새싹식물 6종의 종자에 각 종자별로 대조군 1개군과 실험군 4개군을 구성하여 6MV X-선을 0, 2, 5, 8, 10 Gy 각각 조사하였다. 조사된 종자들을 파종하고 재배하여 식물의 액을 추출 및 성분분석을 실시하였다. 무 순과 적무의 산도는 모두 증가하였고, 청경채와 메밀은 산도가 평균적으로 감소하였다. 다른 종자의 실험 군은 대조군과 비슷한 산도로 분석 되었다 새싹식물의 염도, 당도는 평균적으로 감소하였으나, 적무의 염도는 2배 증가하였고, 수분 함량이 높은 콩 나물은 당도가 모두 증가하였다. 따라서 고 에너지 X선은 식물의 성분에서 염기성 이온을 생성하고, 수분 함량이 높은 식물은 방사선 감수성이 높아 생장에 영향을 받아 과산화물이 생성되어 식물의 성분 변화에 영향을 미친다고 판단된다. 향후 고 에너지 방사선의 조사에 따른 식물성분의 변화 원인을 알아내기 위한 지속적인 유전학적 연구가 필요할 것이다.

This study was conducted to develop a predictive model for the growth of Escherichia coli strain RC-4-D isolated from red kohlrabi sprout seeds. We collected E. coli kinetic growth data during red kohlrabi seed sprouting under isothermal conditions (10, 15, 20, 25, and 30°C). Baranyi model was used as a primary order model for growth data. The maximum growth rate (μmax) and lag-phase duration (LPD) for each temperature (except for 10°C LPD) were determined. Three kinds of secondary models (suboptimal Ratkowsky square-root, Huang model, and Arrhenius-type model) were compared to elucidate the influence of temperature on E. coli growth rate. The model performance measures for three secondary models showed that the suboptimal Huang square-root model was more suitable in the accuracy (1.223) and the suboptimal Ratkowsky square-root model was less in the bias (0.999), respectively. Among three secondary order model used in this study, the suboptimal Ratkowsky square-root model showed best fit for the secondary model for describing the effect of temperature. This model can be utilized to predict E. coli behavior in red kohlrabi sprout production and to conduct microbial risk assessments.

This research was performed to develop mass propagation method of Alpine modest primrose (Primula modesta var. hannasanensis T.Yamaz.) for improving the conservation and utilization of the species. Seeds were collected on August 2011 in Jeju-city and well-selected seeds were dry-stored at 4 ± 1.0℃. Seed size ranged 0.44 ± 0.07 × 0.61 ± 0.04 ㎜, and weight of 1,000 seeds was 51.78 ± 0.021 ㎎. Thus the seed was classified as ‘dwarf seeds’. As the result of dormancy characteristics, moisture content of freshly matured seeds increased rapidly by water-soaking treatment and seeds did not germinate at 20℃ for 4 weeks under the light condition. Therefore, seeds were estimated to have physiological dormancy. Germination conditions of dry-stored seeds were found to be 20℃ and light condition, and seeds did not germinated under dark condition regardless of all temperature regimes. Therefore, Alpine modest primrose seed was considered as photoblastic type. Percent germination (PG) and germination energy (GE) were greatly improved by soaking the seeds in GA3 and kinetin for 24 hours. Especially, 200~500 ㎎/L GA3 treatment resulted in the highest PG (95.5%) and GE (98.3%). So, chemical treatment such as GA3 was thought to be a useful method for raising seedling uniformly.

이 연구는 유기농 수수의 곡물과 새싹채소의 주요 기능성 성분인 항산화성분 및 항산화 활성, 일반성분을 알아봄으로써, 수수의 유기농 농산물로써의 다양한 활용방안에 대한 기초 자료로 알아보고자 실시하였다. 수수의 유광 새싹채소는 종자에 비해 조단백질 함량이 2.5배 증가한 것으로 나타났으며, 무기성분에서도 새싹채소에서 1.6~9.87배의 높은 것으로 나타났 다. 항산화 성분 중 총 polyphenol 함량은 유광 새싹채소에서 평균 4,135 mg GAE g-1으로 높 았으나, flavonoid 함량은 종자에서 평균 3.86 mg GAE g-1으로 함유량이 높은 것으로 조사되 었다. ABTS 라디칼 소거활성은 종자에서 평균 15.78 mg TE g-1으로 높게 나타났으며, 유광 새싹채소와 무광 새싹채소에서는 평균간 차이는 나타나지 않았다. DPPH 라디칼 소거활성에 서는 종자에서 평균 15.79 mg TE g-1으로 새싹채소에 비해 11배 이상의 높은 활성이 나타났 다. 특히, ‘동안메’는 황금찰, 남풍찰 및 소담찰에 비해 종자와 새싹채소에서 가장 높은 활성 이 나타내었다. 항산화성분 및 항산화활성은 품종과는 유의성이 없었으나, 광처리와는 매우 높은 유의성을 보였다. 따라서 유기농 항산화 식품 및 천연 항산화제 공급원의 이용시 종자 (‘동안메’)를 이용하는 것이 좋은 것으로 생각된다. 그리고 수수 새싹채소의 영양학적인 유 기농 식품으로써의 이용에 대한 추가연구가 이루어져야 할 것으로 사료된다.

Seed storage proteins are used as carbon and nitrogen sources for the nutritional improvement of seeds. Since the composition of proteins from the Korean cultivars of proso millet is unknown, this study was conducted to obtain a reference map of millet seed proteins and identify the functional characteristics of the identified proteins. Proteins extracted from proso millet seeds of various cultivars were investigated using proteomic techniques such as 2-D electrophoresis coupled with mass fingerprinting; 1152 (differentially expressed) protein spots were detected on the 2-D gels. Among them, 26 reproducible protein spots were analyzed using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. Out of the 26 proteins, 2 proteins were upregulated in all the millet cultivars, while 13 proteins were upregulated and 11 proteins were downregulated in 2 cultivars. Abundance of most of the identified protein species associated with polysaccharide and starch metabolism, transcription, and pathogenesis was significantly enhanced, while that of other protein species involved in glycolysis, stress response, and transduction was severely reduced. Taken together, the results suggest that the differential expression of the proteins from the four millet cultivars may be cultivar-specific. By conducting a proteomic investigation of millet seeds from different cultivars, we sought to better understand the functional categorization of individual proteins on the basis of their molecular functions. We believe that the identified proteins may help in investigating genetic variations in millet cultivars.

Germination characteristics, seedling emergence, and early seedling growth of iron-coated rice seeds, cultivars Daebo and Samdeokbyeo, under different water depths were compared with those of non-coated seeds (control) and the results evaluated to obtain basic information for establishing stable seedlings in direct water seeding. The total germination percentage of the two seed treatments was similar, but iron-coated seeds had slightly faster germination and shorter mean germination time than non-coated seeds. Water absorption rates of iron-coated seeds were lower than that of non-coated seeds during seed germination. The germination percentage of the two iron-coated rice seed cultivars showed a significant decline of 15-22% after one year of storage under natural conditions. The seedling emergence percentage and uniformity of the two rice cultivars were significantly higher in the iron-coated seeds at 1-13 cm water depths but the percentage of floating seedlings was lower in iron-coated seeds than in non-coated seeds. The iron-coated seeds had a high seedling emergence percentage of 91.3-93.3% at all flooding depths whereas the non-coated seeds had a significantly low seedling emergence percentage of 57.7-71.7% at a water depth of 13 cm. Moreover, the shoot dry weight and seedling health score of iron-coated seeds were significantly higher than those of non-coated seeds, while root dry weights were similar in iron-coated and non-coated seeds, regardless of water depth. These results suggest that iron-coated seeds are more appropriate for stable seedling establishment in direct water seeding than are non-coated seeds.

This study aimed to investigate the morphological characteristics and optimal germination conditions in seeds of Arabis pendula L., a traditionally edible and medicinal plant. The external seed shape was circular-obovate with narrow wings and dark brown. The seed length and width were 1.25 ㎜ and 1.47 ㎜, respectively. The seeds were exalbuminous and the embryo was a bent type. Seed germination was the highest (49.7%) at 20°C under dark conditions among the various temperature and light conditions applied. However, under the dark condition, the seedling was weak, overgrown, and the cotyledons were small and folded. To improve the germination and growth of seedlings, the seeds were pre-treated with GA3 solutions of varying concentrations (0, 200, 500, and 1,000 ㎎/L). The seed germination and seedling growth were effectively improved by GA3 pre-treatment. The germination rate was the highest (97.3%), mean germination time was the shortest (8.1 days), and a vigorous growth of seedlings was observed upon pre-soaking the seeds in 500 ㎎/L GA3 solution. In conclusion, the best method for germination was pre-soaking in 500 ㎎/L GA3 (4℃, dark, 24 h) and incubating the seeds at 20℃ for 15 days.

We compared the germination rate of dehisced ginseng (Panax ginseng) seeds that were dried under two different conditions, slowly at 15℃ [relative humidity (RH) 10-12%] and rapidly under a laminar airflow cabinet at 25℃ (RH 22-25%). The measurements showed that drying rate and seed moisture content (SMC) play important roles in storage ability and vigor. The seeds that were dried rapidly at 25℃ showed high GR compared with the seeds that were dried at 15℃ after 6 and 12 months of storage at -80℃ irrespective of MC. Seeds dried slowly at 15℃ with MC higher than 7.0% showed high GR maintenance after storage at -18℃ and at 4℃ in comparison with rapidly dried seeds. However, the GR of the slowly desiccated seeds decreased as mean SMC was reduced to less than 5.0%, whereas the rapidly dried seeds were distinguished by significantly high GR irrespective of the storage conditions. The ginseng seeds desiccated under different conditions showed differences in storage performance. Seeds with 7-9% MC that were dried slowly at 15℃ for 5-7 days showed high GR after 4℃ and -18℃ storage; however, longer periods of desiccation decreased the germination level remarkably compared with that of rapidly dried seeds.

This research was performed to develop mass propagation method of round-leaved sundew (Drosera rotundifolia L.) for improving horticultural usefulness. Seeds were collected around Hwaseong-si, Gyeonggi-do in October of 2011 and experimented upon while being dry-stored at 4 ± 1.0℃ (darkness). Seed length of a major and minor axis were 1.58 ± 0.060 and 0.21 ± 0.016 ㎜, respecrively, and weight of 1,000 seeds was 6.24 ± 0.172 ㎎. Seeds were thus classified as ‘dwarf seeds’. Regarding the dormancy type, since round-leaved sundew seeds were dormancy broken and germinated at 20~30℃ under the light condition after wet-chilling treatment for 12 weeks, seeds were estimated to have physiological dormancy. Germination conditions of dormancy broken seeds were found to be 20℃ and light condition (54.7%), but germination decreased at higher temperature. Percent germination (PG), germination energy (GE), mean germination time (MGT) and T50 were effectively improved by chemical treatment such as GA3 200 ㎎/L + kinetin 20 ㎎/L and wet-chilling treatment for 14 weeks. In conclusion these optimal conditions were thought to be a useful method for raising seedling uniformly.

Background : Yellowhorn (Xanthoceras sorbifolium Bunge) is distributed in northeastern region in China. The seeds are oil-rich and used as an edible and/or medicinal additives in China. We investigated genetic indices and molecular variance using ISSR markers and oil contents variance by analyzing fatty acid composition in several artificial populations in China. Methods and Results : Seeds were collected from four discontiguous artificial populations in four area in China : two in Inner Mongolia (IM), one in Liaoning (LN) and one in Shandong (SD). Farm in SD showed the highest number of effective alleles (Ne), Shannon index (I), and expected heterozygosity (He), i.e., 1.598, 0.470, and 0.325, respectively. Crude fat contents in kernel observed as 54.5 g 100 g-1 from SD. In contrast it was observed the lowest contents as 46.5 g 100 g-1 from LN . The fatty acid composition was determined to those of oleic acid (31.3%) from SD. And linoleic acid was determined as 38.1% from LN. These artificial populations have relatively high genetic variation, and within-population variation (23%) was higher than among populations. The artificial populations were divided into two groups, revealing these was little correlation between genetic and geographic distance. Conclusion : This study can provide the important information on genetic variation and contents characteristics. It may be responsible for the programs of improvement and germplasm conservation in the future.