최근 천연 유래 미백제에 대한 관심이 증가하고 있다. 천연 유래 미백성분은 ‘자연 유래’라 는 메시지를 소비자에게 호감을 주어 경쟁력을 높인다. 본 연구는 Xanthium strumarium seed 에탄올 추출물의 미백효능을 평가하였다. 그 결과 α-MSH(α-melanocyte stimulating hormone)로 유도한 B16F10에서 티로시나아제와 멜라닌 형성을 억제했다. 또한 핵심조절인자인 티로시나제, MITF (Microphthalmia-associated transcription factor), TRP-1(tyrosinase related protein-1), TRP-2 (tyrosinase related protein-2)에 관여함으로서 최종 멜라닌 생성을 저해하였다. 이러한 결과는 X. strumarium seed 에탄올 추출물의 미백 기능성 성분으로서의 가능성을 시사한다.

본 연구에서는 Chamaecyparis obtusa (Siebold & Zucc.) Endl. (C. obtusa) 잎을 99% 에 탄올 추출(CO99EL)하여 항산화 및 항멜라닌 생성 활성을 평가하여 미백 화장품 성분으로서의 가능성 을 평가하였다. CO99EL은 DPPH 및 ABTS+ 라디칼 소거 활성이 농도 의존적으로 증가하였으며, 티로 시나아제 저해 활성도 유의미하게 나타났다. α-MSH로 유도된 B16F10 세포 모델에서 CO99EL은 12.5 및 25μg/mL 농도에서 세포독성 없이 멜라닌 생성을 유의미하게 억제하고, 세포 내 티로시나아제 활성을 감소시켰다. 또한, qPCR 분석 결과 CO99EL은 MITF, TYRP2, TYR과 같은 주요 멜라닌 생성 관련 유전자의 mRNA 발현을 저해하는 것으로 나타났다. 이러한 결과는 CO99EL이 항산화 및 멜라닌 생성 저해효과를 가진 기능성 화장품 성분으로서의 가능성을 나타낸다.

This study intends to analyze physical and chemical changes using Thermo-Gravimetric Analysis （TGA） of MGO-bioethanol mixed fuel oil. We will analyze the thermal stability and state changes of MGO-Bioethanol mixed fuel oil and conduct and utilize various basic experiments on its applicability as ship fuel oil in the future and eco-friendly alternative fuels. The physical and chemical conditions set through this experiment were set through non-isothermal heating at about 20°C to 933°C, and the heating rate was 100°C/min, the measurement time was 10 minutes, and the amount of samples in each mixed fuel oil was about 18mg-24mg. In the range of pyrolysis temperatures from 235.241°C to 253.320°C, the weight of BE0 was 30.992%, BE10 was 36.199.%, BE20 was 35.879%, and BE30 was 35.725%, indicating that the pyrolysis temperature and weight tended to increase as the bioethanol content increased.

This study investigated the physiological activities of a 70% ethanol extract of jicama by measuring its polyphenol and flavonoid contents, as well as its DPPH and ABTS radical scavenging activities, and α-amylase and α-glucosidase inhibition activities. The polyphenol and flavonoid contents of the extract were found to be 2.45 GAE/g and 3.98 CE/g, respectively. At a concentration of 5 mg/mL, the DPPH and ABTS radical scavenging activities, along with the α-amylase and α-glucosidase inhibition activities, were 52.32%, 45.60%, 47.44%, and 36.96%, respectively.

This experimental study aimed to determine the anti-obesity effects of burdock(Arctium lapp L.) extract and processed (Beopje) burdock extract in a high fat diet-induced obesity model. When burdock extract was orally administered at a concentration of 250 mg/kg BW and the Beopje burdock extract was administered at 250 and 500 mg/kg BW, they significantly decreased body weights increased by a high-fat diet, improved food efficiency ratio, decreased adipose tissue weight by site, and significantly decreased blood triglycerides, total cholesterol, and LDL-cholesterol levels. Blood ALT and AST contents as liver function-related indices increased by the high fat diet were significantly decreased by the Beopje burdock extract. Results of histological analysis of the liver showed that the Beopje burdock extract alleviated fatty liver phenomenon induced by a high-fat diet. In addition, levels of blood TNF-α, IL-6, and IL1-β increased by a high-fat diet were significantly decreased by ALB-L and ALB-H. Therefore, Beopje burdock extract can improve obesity in a high-fat diet-induced obese animal model by improving blood lipids and blood biochemical indices, increasing body water, and decreasing body fat more than the burdock extract.

본 연구는 기능성 화장품 소재로서 생태교란종으로 지정되어있는 가시박 (Sicyos angulatus L., SA)의 이용 가능성을 알아보기 위하여 항산화, 미백, 항균, 항염증 효능 평가를 수행한 후, 에멀션 안정성을 확인하였다. SA는 초순수 (SAW)와 70% EtOH (SAE)로 추출하였다. SAE는 SAW와 비교하 여 DPPH radical 및 ABTS+ radical에 대한 소거 활성과 SOD 유사 활성이 보여 항산화의 효능이 뛰어 나며, tyrosinase의 활성을 저해하여 멜라닌 생성을 억제하였다. SAW는 Escherichia coli (E.c), Staphylococcus epidermidis (S.e), Staphylococcus aureus (S.a), Pseudomonas aeruginosa (P.a), Cutibacterium acnes (C.a) 균주에 대한 항균 효과가 없는 반면, SAE는 S.a를 제외한 E.c, S.e, P.a, C.a 균주에서 clear zone이 생성되어 항균 활성을 나타내었다. LPS를 처리한 RAW 264.7 세포에서 SAE는 SAW보다 iNOS 및 COX-2의 발현량이 억제됨으로써 NO의 생성량을 억제하여 항염증에 대한 효능을 나타내었다. SAE을 첨가한 에멀션을 4℃, 25℃, 50℃의 온도에서 분리 현상이 관찰되지 않고, pH와 점 도는 수치상 큰 변화가 없었으며, 입자크기가 대조군 에멀션의 입자와 유사하므로 제형에 영향을 끼치지 않음을 확인하였다. 본 연구 결과를 기반으로, 기능성 화장품 연구에 새로운 소재로서의 활용 가능성을 제시하고자 한다.

This study intends to use the possibility of an eco-friendly alternative fuel to be applied to ships as a sample manufacturing method for ship MGO and bioethanol mixed fuel oil as basic evidence. The components of the manufactured mixed fuel oil were analyzed using the ISO-8217 standard testing method. As a result of analysis showed that in the lower calorific value decreased to 43030J/g at BE0 fuel and 37010J/g at BE30 fuel. The high calorific value decreased to 46.065MJ/kg at BE0 fuel and 39.460MJ/kg at BE30 fuel. The density decreased to 840.8kg/m3 at BE0 fuel and 837.0kg/m3 at BE30 fuel. In the case of flash point it was 67.5℃ when BE0, and decreased to less than 40.0℃ when BE10 to BE30. Finally the Kinematic Viscosity was 3.011mm2/s at BE0 and decreased to 2.502mm2/s at BE30.

This study investigated major constituents and anti-inflammatory effects of an ethanol extract of Platycodon grandiflorum leaves. Through HPLC analysis, chlorogenic acid and luteolin-7-O-glucoside were identified as predominant constituents in the ethanol extract. Their anti-inflammatory effects were evaluated using murine macrophage (RAW 264.7 cells) and human lung carcinoma cells (NCI-H292 & A549). The ethanol extract significantly (p<0.01) inhibited the production of nitrite, interleukin-6 (IL-6), and prostaglandin E2 (PGE2) induced by lipopolysaccharide (LPS) in RAW 264.7 cells. Furthermore, the ethanol extract suppressed the expression of cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS) proteins in RAW 264.7 cells stimulated with LPS. In NCI-H292 and A549 cells, treatment with the ethanol extract significantly (p<0.05) decreased levels of pro-inflammatory cytokines IL-6 and IL-8 induced by IL-1β. The phosphorylation of ERK rather than JNK in the mitogen-activated protein kinase signaling pathway was observed to be a more important mediator in the down-regulation of pro-inflammatory cytokines in NCI-H292 cells. These findings suggest that the ethanol extract of Platycodon grandiflorum leaves containing luteolin-7-O-glucoside exhibits promising anti-inflammatory properties.

본 연구는 PEBAX/PVDF 복합막을 제조하고 에탄올/물 혼합액에 대한 투과증발 성능을 평가하였다. 또한 PVDF 지지체 표면에 ZIF-8 층을 형성하여 복합막의 투과증발 성능을 향상시키고자 하였고, PEBAX 선택층 두께에 따른 성능 비교 를 통해 최적의 막을 선정하였다. 제작된 복합막을 물과 에탄올이 95/5 중량비로 혼합된 공급액에 대하여 투과증발 실험을 수행하였다. 그 결과 ZIF-8 충이 형성된 PVDF 지지체를 사용한 복합막의 경우 플럭스 1.98 kg/m2h, 분리 계수 3.88로 일반 PVDF 지지체를 사용한 복합막보다 투과량과 선택도가 모두 높은 값을 나타내었다.

This study investigated the anti-obesity effects of Coriandrum sativum L. ethanol extracts in a high fat diet-induced obesity model (DIO). We confirmed the anti-obesity effects by analysing the expression of the related proteins, weight gain, dietary intake, dietary efficiency, blood biochemistry, histological analysis and western blot analysis. After oral administration of Coriandrum sativum L. ethanol extracts at concentrations of 250 and 500 mg/kg, a significant improvement in dietary efficiency, reduction in weight gain, triglycerides, total cholesterol and LDL-cholesterol in blood lipid was observed for 8 weeks. In addition, improvement in blood glucose and metabolism confirmed through glucose tolerance test was observed. Further, the concentration of alanine transaminase (ALT) in blood was significantly decreased, which improved the fatty liver caused by high-fat diet intake as confirmed by liver tissue analysis. This phenomenon was confirmed to decrease the expression of fat accumulation-related PPARγ and FAS protein in the liver tissue. Especially, it is believed that FAS, a liposynthetic enzyme, has a stronger inhibitory effect than PPARγ. Therefore, Coriandrum sativum L. ethanol extract is thought to improve obesity by reducing blood lipids levels, improving glucose metabolism and inhibiting synthesis of the fat that accumulates in the liver in high-fat diet-induced obesity animal models.

‘갑주백목’ (Diospyros kaki Thunb cv. Hachiya) 감은 현재 홍시 또는 건시로만 식용되고 있는데 탈삽된 생과로서의 활용이 기대된다. CO2 및 ethanol 탈삽 처리와 동시에 1-methylcyclopropene (1-MCP)를 처리하여 탈삽과 연화억제에 대한 효과를 보고자 본 연구를 수행하였다. CO2 탈삽은 20L 아크릴 용기를 이용하여 95% CO2를 24시간, ethanol 탈삽은 PE 필름(85.0cm×63.5cm)을 이용하여 99.9% ethanol 20ml를 3일간 상온(23℃)에서 처리하였다. 1-MCP는 탈삽 처리와 동시에 1μL･L-1을 처리하였다. 탈삽 처리 후 상온 모의유통과 3주 저온저장 후 상온 모의유통의 두 가지 조건에서 과실의 품질을 조사하였다. CO2는 처리 종료 후 2일에 ethanol은 5일에 식용이 가능한 상태로 탈삽 되었으며 탈삽이 완료되지 않은 과실은 3주간의 저온 저장에서 완전히 탈삽되었다. 1-MCP는 탈삽속도에는 큰 영향을 미치지 않았으며 CO2와 ethanol 탈삽 모두에서 우수한 연화억제 효과를 나타내었다. 특히, 3주 저온 저장 후 상온 유통에서 1-MCP의 연화억제 효과는 더욱 크게 나타났다. 이상의 결과로 볼 때 1-MCP는 감의 탈삽에 있어 별도의 처리시간을 필요로 하지 않는 효과적인 연화억제 수단으로 판단되며, 이러한 ‘갑주백목’ 탈삽 생과는 향후 국내 유통 및 해외 수출이 기대된다.

본 연구에서는 시엽의 기능성 화장품 소재로서의 활용 가능성을 평가하기 위해 항산화와 인 간섬유아세포인 CCD-986sk 세포에서의 항주름 효과를 알아보았다. 전자공여능 측정, ABTS+ radical 소거능 측정을 통해 항산화 활성을 확인한 결과, 시엽은 1,000 μg/ml 농도에서 대조군인 ascorbic acid 와 농도 의존적으로 유사한 항산화 활성을 보였다. Elastase 저해 활성 측정, Collagenase 저해 활성 측 정을 통해 항주름 효과를 확인한 결과, 시엽은 1,000 μg/ml 농도에서 대조군인 epigallocatechin gallate 와 농도 의존적으로 유사한 항주름 효과를 나타내었다. UVB로 유도된 CCD-986sk 세포 내 pro-collagen type I의 합성률과 MMP-1의 저해률을 측정한 결과 대조군인 EGCG는 20 μg/ml에서 90.2%, 시엽은 30 μg/ml에서 88.5%의 pro-collagen 합성률을 보였다. 또한 EGCG 20 μg/ml와 시엽 30 μg/ml에서 각각 33.0%, 40.8%의 MMP-1 저해율을 확인하였다. Western blot을 통하여 시엽의 pro-collagen type I과 MMP-1의 단백질 발현량을 측정한 결과 UVB 단독군 대비 시엽을 함께 처리했 을 때 pro-collagen type I의 단백질 발현량이 증가하고 MMP-1의 단백질 발현량이 감소하는 것을 확 인하였다. 위 실험결과에 따라 시엽이 UVB 자극에 의한 광노화를 예방하고 항산화, 항주름 효과가 있음 을 확인하여 화장품의 천연물 소재로서의 이용이 기대된다.

To apply UV-C as a non-heating sterilization method to increase the microbiological safety of fresh seedless watermelon products, reductions in E. coli and quality changes by treatment dose (0, 2, 4, 8, 14, 20 kJ/m2) were investigated. The pH, sugar content, and hardness of watermelon inoculated with E. coli were not significantly different according to the UV-C treatment dose, but the polyphenol content was significantly decreased compared to the controls (425.4 GAE μg/g F.W.). When treated with 2 and 4 kJ/m2, the lycopene content was 31.6 and 30.9 μg/g F.W., respectively, which was increased compared to the controls (28.5 μg/g F.W.). The arginine and citrulline content was also significantly increased compared to the controls. The number of E. coli was significantly decreased compared to the controls following UV-C treatment. Considering the degree of E. coli reduction, lycopene content, arginine content, citrulline content, and UV-C irradiation time, subsequent experiments were conducted by selecting a UV-C treatment dose of 2 kJ/m2. The results of confirming the degree of reduction in the number of E. coli colonies by a single treatment and combined treatment with UV-C 2 kJ/m2 and 70% ethanol showed that the combined treatment was most effective as colonies were decreased by 2.3 log CFU/g compared to the controls. Therefore, it is judged that UV-C 2 kJ/m2 radiation and combined treatment with 70% ethanol could be applied as a non-heating sterilization method for fresh watermelon slices.

Diesel engine has the advantages of strong power, low fuel consumption and good durability, so it has been widely used in transportation, automobile, ship and other fields. However, the nitrogen oxides(NOx) and particulate matter(PM) emitted by diesel engines have become one of the main causes of air pollution. Especially during idling, the engine temperature is low, and there are more residual exhaust gases in the combustion chamber, resulting in the formation of more harmful emissions. In this study, performance of a single cylinder, four-stroke, direct injection (DI) diesel engine fueled with diesel–biodiesel mixtures has been experimentally investigated. The findings show that a remarkable improvement in PM–NOx trade-off can be achieved by burning diesel-bioethanol blend fuels.

Diesel engine has the advantages of strong power, low fuel consumption and good durability, so it has been widely used in transportation, automobile, ship and other fields. However, the nitrogen oxides(NOx) and particulate matter(PM) emitted by diesel engines have become one of the main causes of air pollution. Especially during idling, the engine temperature is low, and there are more residual exhaust gases in the combustion chamber, resulting in the formation of more harmful emissions. In this study, performance of a single cylinder, four-stroke, direct injection (DI) diesel engine fueled with diesel–biodiesel mixtures has been experimentally investigated.