Oral verruciform xanthoma (OVX) is an uncommon benign lesion of the oral mucosa, clinically mimicking various verrucous lesions. This study aimed to describe the clinicopathological characteristics of OVX. Twenty-one patients diagnosed with OVX were collected. The patients’ age, sex, medical history, clinical features, and treatment were reviewed. Archived slides were reviewed, and periodic acid-Schiff (PAS) and immunohistochemical (IHC) staining with CD68, CD1a, and S-100 were performed. OVXs affected 13 males and 8 females (M: F=1.6:1) with a mean age of 52 ± 21 years. The most prevalent site was the gingiva (57.1%), followed by the hard palate (28.5%). The lesions presented as an asymptomatic solitary lesion with verrucous or granular surface, with exception of one case with multiple papules on the hard palate. PAS-positive granules were found in xanthoma cells in 14 (66%) specimens. IHC revealed that xanthoma cells were positive for CD68 and negative for CD1a and S-100 in all specimens. Most lesions were successfully excised, and none of the lesions have recurred. Clinicians should include OVX in the differential diagnosis of various verrucous lesions in the oral cavity to provide appropriate management.

Langerhans cell histiocytosis (LCH) is a rare disease that arises from an abnormal increase in histiocytes. Due to its rare occurrence, the diagnosis of LCH is often delayed or missed. This report presents a case of LCH in a 16-month-old girl. After biopsy, the patient’s previous medical records were obtained. The records described the presence of a yellowish plaque on the forehead since birth, a characteristic feature of LCH. Earlier knowledge of this medical history would have helped facilitate the diagnosis of LCH. This report aims to inform clinicians of the clinical and histopathological features of LCH in order to aid in the early diagnosis of this disease, which can occur in infancy.

연구는 갈치 Trichiurus lepturus의 성 성숙과 생식생물학적 기초 정보를 제공하기 위해 수행하 였다. 난자형성과정 동안 난모세포와 핵의 크기는 증가하였으나 핵에 대한 인의 비율은 감소 하였다. H-E 염색 결과, 세포질의 염색성은 호염기성에서 호산성으로 변하였다. 난황형성개시 기 난모세포의 난경은 약 63.2 (±12.7) μm였다. 세포질에서는 호산성의 난황핵이 관찰되었다. 성숙기 난모세포의 난경은 216.6 (±24.7) μm였으며, GVBD (germinal vesicle breakdown)가 관 찰되었다. 완숙기 난모세포의 난경은 317.9 (±80.9) μm였으며, 방사대의 두께는 4.2 (±1.7) μm 였다. 난모세포의 발달형태는 난군동기발달형에 속하며, 난황 축적은 대부분의 경골어류와 마 찬가지로 외재적 방법과 내재적 방법에 의한 것으로 판단되었다.

A 210-day experiment was conducted to examine the effects of starvation on survival, the gonadosomatic index (GSI), hepatosomatic index (HSI), and the intestinosomatic index (ISI), and histological changes in the renal tubule epithelium, midgut epithelium, and hepatocytes in Far Eastern catfish (Silurus asotus). The survival rate decreased to 92.2±0.47% in the fed group and 74.4±2.59% in the starved group during the 210-day experimental period. GSI, HSI, and ISI were lowest in the starved group (p<0.05). The hepatocyte nuclear area, hepatocyte cell area, the nuclear height of the midgut epithelium, and the nuclear height of the kidney were highest in the fed group (p<0.05). The hepatocyte nuclear area, nuclear height of the midgut epithelium, and nuclear height of the kidney were lowest in the starved group (p<0.05). The numbers of melano-macrophages (MMs) found in the kidney cells increased during starvation in this species. This suggests that thinner body cavity regions, the contraction of hepatocyte nuclear sites, and the spreading of kidney cell MMs in this species could be used as alternative indicators for identifying starvation conditions. Therefore, the results from our study provide accurate indications of the nutritional status of Far Eastern catfish.

A 57 years old female complained of severe pain on the right temporomandibular joint (TMJ) area. Her right condyle had been partly resected under surgical operation 13 years ago due to condyle hypertrophy, thereafter she felt dull pain on TMJ area and recently the lesion became severely swelled and painful leading to cancer phobia. The present radiological views showed slightly enlarged and sclerosed condyle with increased radiopacity, but its articular sliding function was almost disable during mouth opening. The patient’s TMJ lesion was carefully managed with conservative physiotherapy and pain treatment. The microsection of condyle head obtained from the previous operation was re-evaluated histologically, and it was finally diagnosed as osteochondrosis dissecans (OCD), exhibiting hyperplastic proliferation of cartilage in condyle head and marked vascular dilatation in epiphyseal zone. This abnormal cartilage tissue was distinguishable from normal cartilage tissue found in the peripheral cartilaginous cap of the same microsection. The involved cartilage cap showed thick hypertrophic chondrocyte zone with horizontal and vertical clefts accompanying diffuse hyaline degeneration. The superficial fibrous zone of cartilage cap was thickened and frequently peeled off, while lower hypertrophic zone of cartilage cap was highly cellular and proliferative. Consequently, the endochondral ossification became aberrant and resulted pre-mature apoptosis of many hypertrophic chondrocytes, followed by diffuse and mild inflammatory reaction in the underlying marrow tissue. Therefore, it was suggested that this hypertrophic condyle lesion, OCD, be differentiated depending on radiological and histological features from ordinary condyle hyperplasia, osteochondroma, and osteoarthritis, and that the pathological confirmation of OCD may provide a reliable modality for dental and medical treatment of chronic and painful TMJ lesion.

This study is for the consideration of the existence tendency of Kudoa septempunctata in olive flounder. In general, muscle has shown a strong PCR positive reaction in spores containing tissues rather than non-containing tissues. However, blood PCR results showed opposed tendency. In various organs of the tested fish containing spores in muscle tissue, heart had shown positive reaction along with muscle at PCR analysis. Muscle fiber necrosis was observed at the histological observation, and this degeneration was common in both samples. The one sample was the PCR positive muscle containing spore and the other was the PCR positive muscle non-containing spore. Both of muscle tissues indicated a positive reaction at ISH (in-situ hybridization) against K. septempunctata.

This study investigated the stress-related metabolites and hormones in blood and compared the muscle structure to identify the reason for blood splash in Hanwoo beef. Five slaughter houses were selected based on the region (Seoul, Gimhae, Jungbu, Naju, and Goryung) and a previous blood splash record. In total, three-hundred eighty blood samples (n=380) and forty-two muscle tissues (n=42) of control and blood splash Hanwoo beef were collected during the slaughter process and beef grading. Blood metabolites were analyzed including glucose, lactate, creatinine, urea-N, and hormones such as cortisol and thyroxin. Muscle fiber, fiber bundle, and capillary wall thickness were measured under microscope. The concentrations of blood glucose, lactate, and urea-N were not significantly different between the control and the blood splash samples. Cortisol and thyroxin levels were not significantly different in both samples. In contrast, the creatinine level was significantly increased (p<0.05) in the blood splash samples. There were also no significant differences observed in muscle fiber, bundles, and capillary wall thickness between the control and the blood splash tissues. In conclusion, blood metabolites, hormones, and muscle fiber showed no differences between the control and the blood splash animal. However, increased creatinine levels may be used as an indicator for identifying blood splash prior to slaughter in Hanwoo.

With the multiple practices of bone graft using different artificial bone regenerative substitutes, the bone graft procedures have been widely performed to increase the bony stabilization of dental implant. Xenogenic bone graft materials have been well developed because of their good biocompatibility and abundant source of bone materials. The present study demonstrated the histological findings from excellent bony remodeling in xenogenic bone graft biopsies compared to those findings in autogenous bone graft. For the graft bone biopsies which were usually done in 5-9 months after graft bone insertion, five types of histological grades including excellent, favorable, partial, degenerative, and poor bony remodeling could be assessed to give prognostic information for dental implant. However, recently the xenograft bone materials have been much improved and produced strong osteogenic effect. Among 239 cases of trephine bur-supported core bone biopsy the excellent bony remodeling was found in 20 cases (13.1%) out of 153 xenogenic bone grafts and in 13 cases (43.3%) out of 30 autogenous bone grafts. They produced abundant new bones on the surface of the graft bones in 5–9 months, and the graft bones were partly resorbed and also surrounded by the repetitive deposition of new bone. The osteophytic new bones showed strong birefringence under polarizing microscope, and were gradually elongated and anastomosed with each other to form trabecular bony networks which became proper stress-baring structures for dental implant. Their marrow stromal tissues were composed of loose connective tissue which was well vascularized but rarely infiltrated with inflammatory cells. The present study compared the histological features of excellent bony remodeling between xenogenic and autogenous bone grafts. Although the ratio of excellent bony remodeling in xenogenic bone graft was still low, 13.1%, the recent advance of xenogeic bone products was remarkable in biological aspect and almost comparable to the autogenous bones. Therefore, it was suggested that the xenogenic bone graft will be applicable to the bone regeneration procedures for dental implant with beneficial output in the near future.

A poor prognosis of oral squamous cell carcinoma (SCC) is partly due to the invasiveness and metastasis of the tumor. One of key elements in tumor invasion and metastasis in the degradation of extracellular matrix is tissue inhibitors of metalloproteinases (TIMPs). This study was performed to determine the expression of TIMP-1 and TIMP-2 of oral SCCs with regard to the histologic invasiveness and differentiation in 5 normal oral mucosa and 36 oral SCCs. The histologic invasiveness of oral SCCs were classified into 4 grades. The differentiation of oral SCCs was divided into 3 grades. The StreptAvidin-Biotin immunohistochemical process, using TIMP-1 and TIMP-2 monoclonal antibodies, was performed to determine the expression of TIMP-1 and TIMP-2. The expression of TIMP-1 was positive in 5 of 17 oral SCCs with weak invasiveness and was positive in 8 of 19 oral SCCs with strong invasiveness. The TIMP-1 expression did not increase significantly with respect to the invasiveness of oral SCCs (P>0.05). The expression of TIMP-2 was strongly positive in 5 out of 17 SCCs with weak invasiveness and was strongly positive in 15 of 19 SCCs with strong invasiveness. The TIMP-2 expression increased significantly with respect to the invasiveness of oral SCCs; the stronger the expression, the stronger the invasiveness (P<0.05). The expression of TIMP-1 and TIMP-2 did not increase significantly with respect to the histologic differentiation. We concluded that with respect to the invasiveness, the TIMP-2 expression increases significantly in oral SCCs but the TIMP-1 expression does not; and that with respect to the histologic differentiation, their expressions do not increase significantly. These results suggested that TIMP-2 can be used as a tool to evaluate the invasiveness of oral SCCs.

수온조절 (6, 9, 12, 15, 18, 21, 24, 27􀆆C)에 의해 유도된 멍게의 물렁증 진행에 따른 생리학적 특성을 연구하였 다. 수온조절에 의한 물렁증 유도율은 수온 15􀆆C에서 가 장 높았으며, 24􀆆C에서 가장 낮았다. 물렁증은 피낭 색깔 및 탄성을 기준으로 S0, S1, S2, S3의 4단계로 구분하였 다. 정상개체와 물렁증을 가진 개체들 사이에서 산소소비 율과 여수율은 유의한 차이를 보였다. S0에 비해 S3단계 에서 혈구 구성비의 가장 뚜렷한 변화는 multi-vacuole cell은 약 1/2 감소하였으며, morula cell은 약 10배 증가 하였다. 기관계 구조의 변화는 피낭, 수관, 새낭, 육질부, 소화선에서 상피세포의 변성, 미세섬유의 변성, 혈구의 증가 및 근섬유의 변형이 확인 되었다. 이러한 모든 특 징들은 멍게의 정상적인 생리학적 기능에 영향을 미칠 수 있다.

Oral squamous cell carcinoma (OSCC) develops through multistep process, that is, from normal mucosa to hyperplastic area and progressed to dysplasia, carcinoma in situ, and finally to invasive carcinoma. The purpose of this study is to investigate the histological types of the transitional area from normal oral mucosa to invasive carcinoma for the baseline data to search intermediate end point markers for early detection of OSCC. For this purpose, we reviewed the 85 patients who were diagnosed as OSCC in the Department of Oral Pathology, Yonsei University College of Dentistry, from 2002 to 2008. We classified these histopathologic findings by light-microscopy, according to the histologic pattern of transitional areas. As results, stepwise transformation from normal oral mucosa, to dysplasia and to OSCC was shown in 47 patients. Intermittent lesions were seen in 16 patients, in which normal oral mucosa, dysplasia, and OSCC were alternately arranged. Twenty two patients showed abruptly transformed to OSCC from normal oral mucosa. These preliminary data will be used for searching biomarkers for early detection of OSCC.

본 연구는 3년생 ‘Fuji Mishima’/M.9의 주간에서 직접 발생한 3년생 주지를 강하게 하수유인한 후 상처를 받았을 것으로 예상되는 만곡부위를 채취하여 하수유인 1, 3, 5, 15일 후의 모습을 관찰하였다. 하수유인 1일 후에는 각 조직이 아직 상처받지 않고 잘 발달된 모습이 관찰되었다. 하지만 3일 후 검경한 결과에서는 하수유인 처리된 하부에서 체관부와 물관부가 다소 눌림 현상이 관찰되었다. 유인 후 5일 후에 검경한 결과, 가지의 상부와 하부 모두에서 체관이 일정한 위치에서 이탈된 모습이 관찰되었다. 유인 15일 후에 하수유인 된 가지의 하부 조직에서 수피조직(주피)까지 파괴된 모습이 관찰되었으며, 체관부의 피해도 특히 심하였고, 이 부위에 새로 형성된 조직이 있는 것이 발견되었다. 유인하지 않은 나무에 대하여 에틸렌 발생량을 측정하였으나 에틸렌은 검출되지 않았고 유인한 가지에서는 처리 3일 후에 에틸렌 발생이 증가하였고 6일 후에는 절반으로 감소하였다.

Bone morphogenetic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway evaluating the ability of BMPs to promote bone formation in grafting procedures and fracture healing. Some studies, have independently reported that sulfated polysaccharides particularly heparin, enhance the osteoblastic differentiation induced by BMPs in vitro, and another study demonstrated that heparin enhanced the bone formation induced by BMP‐2 in vivo. This study was performed to examine adipose stem cell responses to rhBMP‐2 alone and rhBMP‐2 with heparin at 0.25, and 25 μg/㎖ concentrations, respectively, in culture media. Adipose stem cells were cultured for 2, 4, and 8 days toward the osteoblastic differentiation in rhBMP‐2 alone and rhBMP‐2 with heparin at 0.25, and 25 μg/㎖ concentrations, respectively, in culture media. Verification of the stem cell lineage was performed in two ways. The first method was a continuous sequential culture until 5th generation. The second method was using monoclonal antibodies for STRO‐1 and CD 90. Naphthol AS phosphate‐fast blue BB staining for alkaline phosphatase was used for verifying osteoblastic differentiation because Alkaline phosphatase activity had been used as an osteoblastic differentiation marker and degree of osteoblastic activity. Alizarin red staining was also used as an osteoblastic differentiation marker because it quantifies the calcium levels in cells or tissues. During the 5th generation culture, cultured cells actively proliferated, and these cultured cells showed a positive reaction to STRO‐1 and CD90 cell surface molecules. Naphthol AS phosphate‐fast blue BB staining and Alizarin red staining were positive in most samples of each group at 2, and 4 days and positive reaction was proportioned to degree of morphological differentiation. In the concentration of 25 μg/ml of heparin, the ALP activity was highest at the 2nd day in the culture, and then the activities of ALP were decreased significantly at 4, and 8 days. The ALP activity was greatest at the 4th day of the culture, and then decreased significantly at the 8th day in 0 μ g/ml and 0.25 μg/ml of heparin concentrations, Adipose stem cells could be differentiated in rhBMP‐2 in culture media, and the addition of heparin to BMP‐2 promoted differentiation of osteoblasts. Moreover, morphological differentiation was associated with the activity of osteoblasts. This study was shown that, when heparin concentration increases, the early differentiation of the cells was brought about, but the early differentiated cells were rapidly progressed to degenerative changes

The purpose of this study was to evaluate the biological characteristic of deproteinized freeze dried bovine bone(DFDBB) through grafting to maxillary sinus as following time lapsed. Nine patients who were needed of sinus elevation procedure because of severe resorption of maxillary edentulous alveolar bone were selected. patients were divided into three group. Firstly sinus lifting procedure was performed and then the implantation procedure was performed after 6 months in first group, 12 months in second group and 18 months in third group and simutaneously tissues of sinus were obtained by trephine. 18 sections are made from every obtained tissue. 9 sections were stained by Masson's trichrome method and were taken a photo at 100 times of magnification. Relative area of newly formed bone were obtained by IPTK(image processing tool kit) version 5.0 program and mean values and standard deviations were produced from obtained data by using SPSS version 17 program and significance tests were conducted by ANOVA method. This study revealed that DFDBB stimulated new bone formation in maxillary sinus and did not have osteoinductive capacity but osteoconductive capacity, and DFDBB was exceedingly slowly resorbed.

This study was designed to investigate the effects of treadmill exercise of low-intensity and moderate- intensity on the functional recovery and histological change in spinal cord injury (SCI) rats. SCI was induced by the spinal cord impactor dropped after laminectomy. Experimental groups were divided into the Group I (normal control), Group II (non-treatment after SCI induction), Group III (low-intensity treadmill exercise after SCI induction), Group IV (moderate-intensity treadmill exercise after SCI induction). After operation, rats were tested at modified Tarlov scale at 2 days with divided into 4 groups, and motor behavior test (BBB locomotor rating scale, Grid walk test) was examined at 3, 7, 14, and 21 days. For the observation of damage change and size of the organized surface in spinal cord, histopathological studies were performed at 21 days by H & E, and BDNF(brain-derived neutrophic factor) & Trk-b immunohistochemistry studies were performed at 1, 3, 7, 14, 21 days. According to the results, treadmill exercise can play a role in facilitating recovery of locomotion following spinal cord injury. Specially, moderate-intensity treadmill exercise after SCI induction was most improvement in functional recovery and histological change.

Nowaday many researches has proved that glutaradehyde(GA) is more excell ent medicament in vital p띠 potomy practice than formocresol (FC) . But a number of dental practitioner prefer to use formocresol in vital pulpotomy procedure todays And thus author reeva luate proper ties of gluteraldehyde and formocresol through implantation into epidermis and trypsin digestion after f ixation at 2% buffered glutar aldehyde and Burkley's formocresol solution.. And then prepared t issues were s tained by H&E a nd Masson-TI’ ichome method. GA showed definite fixative zone and γery low diffusi ble property in epi dermis and pulp t issue as glutaraldehyde and formocresol were compared. GA r epresented exceedingly lower antigenici ty than FC GA fixation exhibited more resistibility to trypsin digestion than FC. As considering these results‘ it concluded that GA would be extremely supe ri 이 . medicament to formocresol in vital pulpotomy proce

The purpose of this study was to evaluate the effects of pulsed ultrasound on wound healing and observe during the wound healing process the distribution of mast cells according to histopathologic findings. Eighty Sprague Dawley rats which were divided into 4 groups received full thickness skin wounds on the back. Each of the 5 animals was sacrificed immediately and then sacrificed again 1, 3, 6, and 12 days after injury. Specimens from the wounds were removed during healing and routinely processed with a hematoxylin-eosin stain and a toluidine blue stain. The authors then observed the distribution of mast cells under a light microscope. The results of this study were as follows: The rate of wound healing and the length of the wounds of the pulsed ultrasound group II was significantly faster than group I on day 6 and day 12 (p<.001). Group III showed the most significant effect after12 days (p<.001). Group IV also showed a significant effect at 12 days (p<.01). A low-intensity ultrasound .5 resulted in a fast healing rate. During the wound healing process mast cells had a tendency to decrease in the acute inflammatory phase. During the wound healing process mast cells were thought to contribute to the healing of the wound.