산화적 스트레스는 세포 및 조직 손상을 통해 피부의 탄력 및 보습 기능 저하, 피부 노화 촉진 을 비롯한 다양한 피부질환을 일으킨다. 본 연구의 목적은 인간 피부각질세포 (HaCaT keratinocyte)에서 산화적 스트레스에 대한 붉은 토끼풀 추출물의 효능을 검토하여, 피부에 효과적으로 사용할 수 있는 기능 성 소재로서의 활용 여부를 확인하고자 하였다. 본 연구에서는 붉은 토끼풀 추출물이 인간 피부각질세포에 서 산화적 스트레스에 따른 세포사를 억제시키는 것을 확인하여, 이를 조절하는 보호기전을 규명하였다. 이는 붉은 토끼풀 추출물이 Caspase-3 비활성, 세포사 촉진단백질 Bax 발현 억제, 세포생존 촉진단백질 Bcl-2 발현 증가 및 MAPK 신호전달계 단백질의 인산화 억제를 통해 H2O2에 의해 유도된 산화적 스트레 스를 보호할 수 있다는 것을 확인하였다. 따라서 붉은 토끼풀 추출물은 피부의 산화적 손상을 감소시키는 유용한 소재로 평가되며, 이는 피부보호 및 미용을 위한 다양한 제품 및 산업에 활용 가능성이 높은 것으로 판단된다.

This study evaluated the nutritional composition and quality traits of rabbit meat as compared to chicken meat. Samples of loin (M. longissimus dorsi) and breast meats were collected from rabbit and chicken carcasses, respectively. The meats were then analyzed for the proximate composition, collagen and energy contents, fatty acid composition, myoglobin and heme iron contents, pH value, water-holding capacity (WHC), cooking loss, meat color, Warner-Bratzler shear force (WBSF) value, and texture profile. Compared to chicken breast meat, lower (p<0.05) protein content and higher (p<0.05) ash and collagen contents were obtained in rabbit loin meat. Rabbit meat remarkably had higher (p<0.05) total polyunsaturated fatty acids (PUFA) and linolenic acid contents and lower (p<0.05) n-6/n-3 PUFA ratio as compared to chicken meat. The pH value and WHC were lower (p<0.05) in rabbit meat than in chicken meat (p<0.05). Rabbit meat exhibited lower (p<0.05) L* value and higher (p<0.05) a* and b* values compared to chicken meat (p<0.05). The WBSF value, hardness, and gumminess were higher (p<0.05) in rabbit meat than in chicken meat (p<0.05). These findings suggest that rabbit meat has higher essential n-3 PUFA, darker color, and firmer texture as compared to chicken meat.

We observed MMPs expression in all sperm groups, with pro-MMP showing lower expression than active MMPs. According to the results from each freezing extender, the sperm membrane integrity (HOST: Hypoosmotic Swelling Test) analysis in TCGGD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Glycerol 3%, Dimethylsulpoxide 3.5 M) is 59.8 ± 0.7, TCGSD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Sucrose 0.1 M, Dimethylsulpoxide 3.5 M) is 59.3 ± 0.5 were significantly higher (p < 0.05) among the experimental groups. And MMPs analysis result, we observed MMPs expression in all sperm groups, with pro-MMP showing lower expression than active MMPs. The expression of active MMP-2 was the highest in sperms frozen in TCGSD and TCGD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Dimethylsulpoxide 3.5 M), Meanwhile, sperms from the TCGGD and TCGED (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Ethylene glycol 3%, Dimethylsulpoxide 3.5 M) group showed lower level of active MMP-2 expression. Together, these results indicate that adding glycerol or sucrose to the sperm freezing buffer would not only suppress MMPs expression but also minimize DNA fragmentation, providing a mean to improve the success rate in the in vitro manipulation of rabbit sperms. Therefore, these results suggest that TCGGD or TCGSD extender method for freezing-thawing of rabbit sperm increased the viability after thawing.

온대지역에 분포하는 박쥐가 생존을 위하여 선택하는 동면은 에너지가 고갈되는 시기에 직면하는 에너지 문제해결을 위한 적응현상이다. 본 연구에서 온대지역에 분포하는 토끼박쥐의 온도선호도와 동면전략 (동면기간)에 대한 연구를 수행하였다. 박쥐의 온도선호도와 동면전략과의 연관성을 알아보기 위하여 박쥐의 온도선호도는 동면기간에 영향을 준다는 가설 검증을 하였다. 이를 위하여 토끼박쥐의 분포를 확인하였고 동면처의 환경특성 및 토끼박쥐의 온도선호도를 도출하였다. 또한 토끼박쥐는 외부 최저 기온이 온도선호도보다 낮아지는 시기에 동면처에 도착하여 외부 최저 기온이 온도선호도보다 높아지는 시기에 동면처를 떠날 것으로 예측하였다. 동면중인 토끼박쥐의 평균 체온은 3.03±1.30℃ (range 0.1~7.6℃, n=179)로 대기온도 (Ta)에 비해 암벽온도 (Tr)와 밀접하게 상관되었다. 토끼박쥐는 외부 기온이 온도선호도보다 낮아지는 11월 중순 이후에 동면처에 도착하여 115~120일 동안 동면처에 머물렀다. 또한 외부 기온이 온도선호도보다 높아지는 시기인 3월 중순 이후에 동면처를 떠났다. 본 연구의 결과는 토끼박쥐의 동면기간은 대상 종의 온도선호도와 외부기온과의 상호작용에 의한 것임을 시사하며 또한 대상종의 온도선호도 및 동면기간 같은 동면전략은 대상 종의 분포 제한 요인으로 작용될 수 있다.

캐나다 시의 정원에 뿌려진 씨앗 중에서 예이츠가 1차세계대전 이후의 캐나다의 대표적 시인들과 문학운동에 미친 직간접적 영향은 무시할 수 없다. 이 논문 은 아직 예이츠 연구분야에서 잘 다루어지지 않은 예이츠의 북미 강연여행(1903-4년)의 흔적을 찾고자 한다. 그리고 캐나다 본연의 목소리를 내고자 하는 작가들에게 캐나 다의 몇몇 도시에서 행한 예이츠의 강연이 어떤 영감을 주는 지 연구할 것이다. G.D. 로버츠와 블리스 카먼의 작품에서처럼 예이츠와 그의 작품에 대한 수많은 비유와 비 교점은 캐나다의 시에 깊이 베어있다. 시의 요람으로서 캐나다 남부와 몬트리올의 맥 길대은 캐나다의 모더니스트 시인들을 생산하는데, 스미스를 대표로 하는 맥길그룹은 A.J.M 스미스와 F.R. 스콧이 편집하는 􋺷맥길 격주 리뷰􋺸와 같은 군소 문예지를 통해 서 캐나다의 시에 체계적인 모더니즘 운동을 소개한다. 특히 스미스는 이전의 캐나다 의 시 기법에 아일랜드의 모더니스트적 기법을 녹여서 시의 혁신을 가져온다. 스미스 자신의 작품은 시작법과 강한 어조는 쉽게 예이츠의 시를 연상시킨다. 이 논문은 예이 츠와 아일랜드 문예부흥운동이 캐나다의 모더니스트 시 운동에 미친 가닥을 찾아서 문학의 직물을 짜고자 한다.

X-ray diffraction studies have been made to investigate the effects of binding of ADP, ADP+Vi, ADP+AIF4, ADP+BeF3 on the structure of glycerinated rabbit skeletal muscle in the rigor state. Although these phosphate analogs are known to bind actively cycling myosin heads, it is not clear whether they can bind to the attached heads in the rigor muscle. We have found that these analogs can bind to the myosin heads attached to actin filaments in the rigor state. The present results indicate that (1) bound myosin heads altered their conformation in the proximal end toward the plane perpendicular to the fiber axis when MgADP bound to them, and (2) myosin heads were dissociated substantially (up to 50%) from actin filaments but still remained in the vicinity of actin filaments when MgADP and metallofluorides (AIF4 and BeF3) or vanadate bound to them. We detected new conformations of myosin heads attached to actin filaments when they had MgADP or ADP.Pi analogs. We report here these findings on the effects of MgADP and MgADP+phosphate analogs to the rigor crossbridges.

본 연구는 New Zealand White 수토끼에서 춘기발동 기간 동안에 혈청내 IGF-I(insulin-like growth factor-I)과 GH(growth hormone) 수준의 변화, 정자 형성에 따른 정소내 세포 구성 변화 및 이들 측정치들 간에 관계를 조사하기 위하여 실시되었다. 주령과 관련된 정소내 세포의 DNA 함량 변화 조사를 위하여 10~28주령 수토끼 정소 조직의 fine-needle biopsy를 flow cytometry(FCM)로 분석하였다. 생체중은 12~20주령 때 크게 증가되었고(P<0.05), 28주령 체중은 3.4kg이었다. 혈청 IGF-I 수준(451.3ng/mL)은 20주령에서 가장 높았으며(P<0.05), 그 후 낮은 수준으로 유지되었다. 혈청 GH 수준은 183.3pg/mL으로 다른 주령 때보다 현저히 높았으며(P<0.05), 상승시기가 IGF-I보다는 다소 빨랐다. 정소 조직세포 중 1C-세포의 상대적 비율은 18주령 때 48.2%로 16주령보다 크게 상승되었고(P<0.05), 주령 증가와 더불어 68%로 증가되었다. 2C-세포 비율은 18주령 때 26.8%로 16주령의 54.3%보다 현저히 낮았다(P<0.05). 4C-세포 비율은 18주령 때 9.9%를 제외하고 2~6%를 유지하였다. 이상 결과에서 토끼는 춘기 발동 개시가 약 18주령에 일어나고 이 기간 중 IGF-I과 GH 수준의 변화가 나이 또는 체성장과 관계가 있었으며 그 영향이 정자 형성과 관련이 있음을 알 수 있었다. Fine-needle biopsy와 연계된 FCM은 춘기 발동 개시와 관련된 정자의 형성 과정을 평가하는데 매우 정확한 방법임을 확인할 수 있었다.

MT-GHR(Growth hormone receptor)와 MT-IGF-IR(IGF-1 receptor)유전자를 구축하고 micromani-pulator를 이용하여 토끼 수정란에 유전자를 주입하여 형질전환토끼를 생산하였다. 본 연구에서의 형질전환토끼의 생산효율은 약 3%를 나타내었고 Growth Hormone receptor(GHR)를 가진 형질전환 토끼와 IGF-1 receptor(IGF-lR)를 가진 형질전환토끼를 10마리 이상씩 생산하였다. 또한 정상 토끼와 교배시켜 F₁ 새끼를 얻어 유전자가 다음세대에도 전달되는 것을 확인하였다. GHR 이나 IGF-1R 형질전환토끼의 성장률은 정상토끼보다는 약15∼25% 정도 빠른 경향을 나타냈고 특히 GHR 형질전환토끼의 성장률이 더 높은 것으로 드러나 GHR 및 IGF-lR유전자가 형질전환토끼에서 성장에 영향을 주었다는 것을 확인할 수 있었다.

The purpose of this research was to determine the effects on the healing of fibular fractures in rabbits of low-intensity pulsed ultrasound (50 and 500 ) applied for periods of 4, 14 and 24 days following fibular osteotomy. Thirty-six male Japanese white rabbits were randomly divided into three groups of twelve for three treatment protocols: (1) ultrasound treatment at intensities of 50 and 500 until the 4th day following fibular osteotomy, (2) ultrasound treatment at intensities of 50 and 500 until the 14th day following fibular osteotomy, and (3) ultrasound treatment at intensities of 50 and 500 until the 24th day following fibular osteotomy. The low-intensity pulsed ultrasound was applied to only one fibula of each rabbit (these served as the experimental group). The other fibula of each rabbit served as the control group. The selection of which fibula was to be treated was made randomly. The animals were sacrificed on the 4th, 14th and 24th day after the start of ultrasound treatments. Percent of trabecular bone area and fibular radiography were carried out to compare the degree of fibular bone healing. A microscope was also used to determine any histologic changes. For statistical differences in radiological changes due to length of treatment period (4, 14 and 24 days respectively), the Wilcoxon signed-ranks test was used to compare the experimental and control groups. For statistical differences in fracture healing due to differences in ultrasound intensity, radiological studies were compared using the Mann-Whitney Test. And, to compute percentage differences in areas of trabecular bone, Two-way analysis of variance (ultrasound intensity x each group) was used. Experiment results were as follows: 1. In animals sacrificed on the 4th day, no difference was found in the radiological studies of the fibulae in the experimental and control groups (p>.05). However, experimental groups showed more rapid bone repair than control group. 2. Both radiographic and percent of trabecular bone area studies showed significant differences in rabbits sacrificed after 14 days. Fracture healing was significantly increased in the experimental group (p<.05) 3. In the animals sacrificed on the 24th day, histologic study showed rapid bone repair but fibular radiologic studies did not show statistical differences between the two groups (p>.05). 4. On the 14th day, bone union on radiograph was significantly more rapid in the treatment group with pulsed ultrasound of 50 than the group with 500 (p<.05). Histologic studies showed that both the 14 and 24 days groups had more rapid bone repair in animals treated with 50 ultrasound intensity than those treated with 500 intensity. In conclusion, it has been shown that the low-intensity pulsed ultrasound has a positive effect on bone fracture healing in the early stage and the range of pulse ultrasound from 50 to 500 is effective for fracture healing. Further study is needed to investigate the influence of pulsed ultrasound on delayed union and non-union in bone fractures and also for the clinical use of low-intensity pulsed ultrasound for bone healing in humans.

Transgenic rabbits were produced by DNA microinjection using growth hormone receptor (GHR) and IGF-1 receptor (IGF-1R) genes. Overall efficiencies for production of transgenic rabbits were 3.2% and 3.1% in GHR and IGF-1R genes, respectively. Founder rabbits transmitted transgenes to their progenies through medelian fashion. Growth rate in GHR and ICF-1R transgenic rabbits was faster than non-transgenic rabbits. Transgenic rabbits grew larger (25% and 15% increase in body weight of GHR and IGF-1R transgenic rabbits, respectively) than non-transgenic rabbits and organ weight of transgenic rabbits increased, suggesting that GHR and IGF-1 genes affects growth rates in transgenic rabbits.

The principal objective of this study was to clone transgenic embryos in order to improve the efficiency of transgenic animal production by the combination of microinjection and nuclear transplantation techniques. Mature female New Zealand White rabbits were superovulated by eCG and hCG treatments, fllowed by natural mating. Zygotes were collected from the oviducts at 18∼22 h after hCG injection by flushing with D-PBS containing 5% fetal calf serum(FCS). Two to three picoliters of green fluorescent protein(GFP) gene wa microinjected into male pronucleus. The foreign gene-injected zygotes were cultured in TCM-199 or RD medium containing 10% FCS with a monolayer of rabbit oviductal epithelial cells in a 5% CO2 incubator. The morulae expressing GFP gene were selected and their blastomeres were separated for the use of nuclear donor. Following nuclear transplantation of fluorescence-positive morula stage blastomeres, 13 (21.3%) out of 61 fused oocytes developed to blastocyst stage and all of the cloned blastocysts expressed GFP. The results indicate that the screening of transgene in rabbit embryos by GFP detection could be a promisible method for the preselection of transgenic embryos. Also the cloning of preselected transgenic embryos by nuclear transplantatin could be efficiently applied to the multiple production of transgenic animals.

To investigate the effects of bacterial endotoxin on lipids and cytokines, and to explain the relationships between their changes, we carried out this study using experimentally Escherichia coli (E. coli) endotoxin-induced septicemic rabbits. The triglyceride and cholesterol concentrations in endotoxin groups were generally high (p$lt;0.01 or 0.05) at all sampling time points (from 3hr to 24 hr), while phospholipid concentrations in endotoxin groups elevated in dose-dependent fashion at 3hr and 6hr after endotoxin injection compared to control group (p$lt;0.05). There were significant negative correlations between lipids changes and cytokines (TNF-α and IL-1β) each other (p$lt;0.05), and endotoxic rabbits having higher triglyceride of cholesterol levels shown relatively better conditions compared with others. As a result, the alterations in the lipid compositions caused by endotoxin may imply an active exhibition of the host defense mechanism rather than the disturbances of lipid metabolism.