Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA), a chronic contagious disease in small ruminants. The prevalence of CLA has been reported to be >50% in Korean black goats. CLA is difficult to control due to a lack of efficient vaccines and treatment methods. Effective disinfection of the farm environment may be an alternative strategy for reducing the spread of C. pseudotuberculosis. The objective of this study was to evaluate the efficacy of commercial disinfectants against CLA. The six commercial disinfectants, largely composed of sodium dichloroisocyanurate, sodium hypochlorite, potassium monopersulfate triple salt, quaternary ammonium, citric acid, and copper sulfate, were tested against five different genotypes of C. pseudotuberculosis isolated from goat farms in Korea. Efficacy tests were performed in accordance with the disinfectant efficacy test guidelines recommended by the Animal and Plant Quarantine Agency of Korea with slight modifications. All disinfectants except for copper sulfate exhibited >99.99% killing efficacy under hard water conditions following 30 min of incubation, which is the recommended standard treatment time according to guidelines. The minimum bactericidal treatment time was evaluated by employing treatments for durations of 1, 5, and 15 min. The most effective compounds under hard water conditions were sodium dichloroisocyanurate, potassium monopersulfate triple salt, and sodium hypochlorite, exhibiting >99.99% killing efficacy after 1 min of treatment. In the aqueous solution forms, citric acid and the quaternary ammonium compound were the most effective, but required at least 5 min to kill >99.99% of the bacteria. The current study characterizes the killing efficacy of six commercial disinfectant active compounds against C. pseudotuberculosis. Thus, this study provides essential information regarding the efficacy of the disinfectants used to control CLA in goat farms.

This study was conducted to evaluate the forage productivity and feed value of Sasa borealis (S. borealis) using growing black goats(S. borealis) in order to improve the utilization of S. borealis and to help mitigate the problem of reduced plant species diversity caused by S. borealis in Hanlla Mountain. One control and three treatments were made by the level of addition of S. borealis silage to the TMR feed. T1(10%), T2(20%), and T3(30%) treatments showed more daily weight gains than control group. Feed conversion ratio of T2 is 4.4g, which is significantly lower than control(P<0.05). The nitrogen retention in the control, which had relatively high dry matter intake, was 12.5g, which was significantly higher than that of T3. Sasa. borealis silage is considered to be able to use as a forage source for black goats, and if it is fed in an appropriate amount, it is considered that it will help improve livestock productivity, such as weight gain and feed conversion ratio.

본 연구는 천연 기능성 소재인 비파잎의 첨가가 흑염소고기 저지방 소시지의 품질특성 및 항산화 활성에 미치는 영향을 알아보기 위하여 실시하였다. 20% 지방을 첨가한 대조구(control), 10% 지방을 첨가한 처리구(LF), 10% 지방과 0.25% 비파잎분말을 첨가한 처리구(LL)로 처리하였다. 저지방 소시지인 LF, LL은 대조구보다 지방함량은 낮고, 수분과 단백질 함량은 높았고, 보수력도 향상되었다(p<0.05). 육색의 경우 비파잎을 첨가한 LL은 적색도와 황색도가 감소하였고(p<0.05), TPA에서 탄력성, 검성, 씹힘성 및 응집성은 LF보다 증가하였고, 대조구와 유사한 조직감을 갖는 것으로 나타났다(p<0.05). TBARS와 DPPH radical 소거능은 비파잎을 첨가한 LL에서 우수한 항산화 활성을 나타냈다. 따라서 기능성 소재인 비파잎을 첨가한 흑염소고기 저지방 소시지는 고단백, 저지방 특성의 흑염소고기 섭취 기회를 늘려줌과 동시에 건강 지향적 육제품 개발의 기초자료로 활용될 수 있다.

The present study aimed to investigate the conceptus-related changes during early pregnancy in the Black Bengal breed of goat. A total of 22 gravid genitalia of the Black Bengal goats were collected from local slaughterhouses. The crown-rump lengths (CRL) of the conceptuses were determined to estimate the gestational age (GA). The length and diameter of uterine horn and amniotic sac were measured, and volume of amniotic and allantoic fluid formed by individual conceptus were recorded. The results reveal that the CRL is positively correlated with GA of the conceptus (R2 = 0.89, p < 0.05); however, CRL was not influenced by number of conceptus. Both the left and the right uterine horn gradually increased in size with the advancement of pregnancy irrespective of conceptus number present in the horn. The size of the amniotic sac of conceptus gradually increased with the conceptus age but maintained spherical shape from 5 to 7 weeks of pregnancy. The amniotic fluid formed by individual conceptus rapidly increased from 5 weeks (3.4 ± 0.3 mL) to 7 weeks (21.0 ± 2.0 mL) and 9 weeks (111.5 ± 4.0 mL). The volume of allantoic fluid formed by individual conceptus was steadily increased until 7 weeks (60.0 ± 5.0 mL) and began to decline slowly thereafter (50.0 ± 5.0 mL at 9 weeks). Notably, there was no effect of conceptus number per pregnancy on individual amniotic and allantoic fluid volume. The cotyledons have first appeared on the allanto-chorionic surface from 4 to 5 weeks of pregnancy. The closed eye, nostril and hooves of the conceptus became visible at 7 weeks of pregnancy. The present study has shown the basic information on conceptus-related developmental changes during early pregnancy up to 9 weeks in Black Bengal goat.

본 연구에 이용되는 국내 재래 흑염소는 유전적 자원을 보존하고 품종을 유지하기 위하여 소규모 집단에서 고도의 근친교배를 수행하고 있어 유전적 자질의 다양성이 저해되고 있을 것으로 우려되었다. 따라서 본 연구는 국내 재래흑염소 경상대계통 집단의 유전체 정보를 활용하여 재래흑염소 집단의 유전적 특성과 유효집단의 크기를 추정하고자 실시하였다. 국내 재래 흑염소의 유전체 정보는 Illumina Goat SNP 50k chip (illumina, inc., San Diego, CA)의 정보를 분석하여 연구에 이용하였다. 각 염색체의 인접 표지인자 와의 연관불평형 (Linkage Disequilibrium)은 0.225로 추정이 되었다. 또한, 표지인자 사이의 거리가 증가함에 따라 연관불평형의 값은 감소되는 것으로 나타났다. 국내 재래 흑염소의 유효집단크기는 최근의 세대로 오는 경우 감소하는 추세를 보였으며, 13세대에서 유효집단의 크기는 29두로 추정되었다. 재래 흑염소 경상대계통은 낮은 연관불평형 값과 유효집단 크기를 보여 유전적 자원의 다양성이 낮을 것으로 보이며, 이를 해결하기 위한 계획적인 교배와 품종 집단의 크기를 키우기 위한 대책이 필요할 것으로 사료된다.

The aim of this study was to assess the levels of genetic diversity and relationships of Korean native black goat (n = 58), compared with the exotic breed, Boer (n = 97). For the analysis of genetic characterization 11 microsatellite markers (MAF065, INRA063, CSRD247, OarFCB20, SRCRSP5, INRA006, ILSTS008, ILSTS011, INRA005, ILSTS087, SRCRSP8) were genotyped. The number of alleles was observed 3 (INRA005) to 10 (SRCRSP8) each markers. The mean expected and observed heterozygosity (Hexp and Hobs) and polymorphism information content (PIC) for the Korean native black goat breed varied from 0.551 to 0.860, 0.517 to 0.948 and 0.464 to 0.835, respectively. Principal Components Analysis (PCoA) and FCA results showed that Korean native black goat breed was confirmed to be clearly separated from bore breed. These results were scientific evidence that Korean native black goat represents a unique and valuable animal genetic resource.

This study was conducted to determine grazing intensity of growing Korean native goats(Capra hircus coreanae) on mountainous pasture. It was carried out to obtain basic information for improvement of mountainous pasture management and establishing feeding system of Korean native goat. A total of 20 goats were grouped by feeding systems [A mountainous pasture grazing group (Concentrated feed of 1.5% body weight, treatment 1, T1, n=10) and a barn feeding group (TMR, treatment 2, T2), n=10] to conduct study from April to September. The average forage productivity of the mountain pasture was 500.9 ± 61.41 kg/ha. The average dry matter intake in T1 was 0.64 and the calculated grazing intensity was 21 head/ha. In productivity, when the two treatments(T1, T2) were compared, the dry matter intake was about two to three times the difference. The average daily gain per day during the experiment was 63.3 in the mountain pasture and 120 g in barn feeding. When grazing, considering mountainous pasture productivity it is necessary to increase the productivity through proper feeding. The feed costs of black goats raised by grazing on the grassland in the same period showed an average 75% reduction compared to barn feeding. As a result of this study, it can be expected that a considerable reduction of feed costs can be expected in the breeding of Korean native black goat using the mountain pasture.

The study was conducted to determine effects on forage productivity, feed value, grazing intensity and livestock productivity in growing Korean native female goat grazing in native pasture. Its with average initial body weight of 14.10±3.6kg and an average age of 4 months were used in this study. Dry matter content of native pasture was the highest at 33.48 ± 2.56% in June, and the content was significantly increased from spring to autumn (p<0.05). Crude protein was maintained between 11% and 12% on average. Nutrient content was maintained at a certain level in native pasture, but there were differences due to the different types of wild grasses produced in each season. The productivity of forage crops increased from June, but decreased after August and showed a characteristic of grassland where productivity decreased rapidly in spring and autumn. The average grazing intensity are 39 head/ha. Black goat average daily gain was 80.2g/d. The stable weight gain in grazing is that the nutrient requirement of the black goat was met by supplementing the concentrated feed during grazing. study, can be expected that the productivity of livestock can be increased through the proper feeding of supplementary feed and maintenance of grazing intensity

The aim of this research was to investigate the anatomical location of muscles and their meat quality traits, including muscle pH, water-holding capacity, meat color, tenderness, and sarcomere length, of Korean native black goat(KNBG). Five male KNBGs of 18 months of age were slaughtered and four major muscles-longissimus dorsi(LD), psoas major(PM), semimembranosus (SM), and gluteus medius(GM)-were sampled to determine proximate analysis and meat quality traits. There were no significant(p>0.05) differences in chemical components among the four muscles. The water-holding capacity(WHC) of the four muscles was significantly(p<0.05) affected by muscle type, although the pHu and meat color were not significantly(p>0.05) different among the four muscles. The Warner-Bratzler shear force of PM(4.07 kg/㎠) was significantly(p<0.05) lower than that of SM(5.57 kg/㎠) muscle. In addition, the sarcomere length of PM(1.99 ㎛) was significantly(p<0.05) longer than that of SM(1.72 ㎛) and LD (1.76 ㎛). Results suggest that the lower shear force of PM muscle is due to its longer sarcomere length.

The objective of this experiment was to investigate amino acids and mineral compositions of the four major muscles (LD: longissimus dorsi, PM: psoas major, SM: semimembranosus, and GM: gluteus medius) from Korean native black goat (KNBG). Five uncastrated male KNBGs of 36 months of age were commercially slaughtered and the four muscles were sampled to determine concentrations of myoglobin, collagen, amino acids and minerals. There were significant differences (p<0.05) in myoglobin and collagen content among the four muscles. Myoglobin content of PM was significantly higher (p<0.05) than those of SM and GM. Collagen content of SM was significantly higher (p<0.05) than that of LD. There were significant differences (p<0.05) in cystine and leucine concentrations among the four muscles, and PM had the highest concentrations of the two amino acids compared to other muscles (p<0.05). Also, there were significant differences (p<0.05) in K, P, Mg and Fe contents among the four muscles from KNBG. PM had the highest mineral content while the lowest mineral content was observed in LD. In addition, Fe contents of PM and GM were higher than those of LD and SM (p<0.05). These results indicated that amino acids and mineral content vary considerably with the anatomical location of muscles of Korean native black goat.

This study was conducted to evaluate the protein requirement for maintenance of fattening Korean black goat (Capra hircus coreanae). Six male goats with average initial body weight (BW) of 31.78±4.54kg and an average age of 8 months were used in this study. The experiment had a replicated duplicated 3×3 Latin square design for balancing carryover effects. In the course of the experiment, each of Black goats were fed three diets that were formulated to contain T1 (13%), T2 (16%) and T3 (19%) levels of crude protein (CP). A 14-day diet adjustment period was followed by a 5-day collection period. Dry matter intake (DMI) of groups fed diets with T2 was 966.67g/d which was higher than group fed diets with T1 and T3 were 925.14g/d and 936.08g/d each. Average daily gains (ADG) of black goats were the highest in T2(167.13g/d) But, there was no significant difference. Dietary protein levels affected the apparent digestibility of CP (p<0.05). A significant difference was found in CP intake among treatments and goats receiving T3, T2, and T1 recorded 181.23, 154.57, and 128.78g CP/d, respectively. This was excepted because CP intake is proportional to CP content of diet, which from highest to lowest was as follows: T3 (19%) > T2 (16%) > T1 (13%). Intercept of the regression equation between CP intake and CP balance indicated that maintenance CP requirement was 1.63g/BW0.75.

This study was conducted to determine grazing intensity of growing Korean native goats (Capra hircus coreanae) on mountainous pasture. It was carried out to obtain basic information for improvement of mountainous pasture management and establishing feeding system of Korean native goats. Castrated, male goats (n=10) with average initial body weight (BW) of 23.33±2.15kg and an average age of 4 months were used in this study. Grazing goats were supplemented by concentrates with 1.0% of BW. The crude protein content of forage was the highest in October (22.71±0.25%) and there were significantly differences (p<0.05) in monthly comparison. The forage productivity of pasture was the highest from May to June (1718.7±207.5~ 1672.0±422.8 kg/ha) but it was decreased in July (1356.0±103.8 kg/ha) because of drought and summer depression. Average daily gains (ADG) of goats were the highest in June (99.5±6.4 d/g). Grazing intensity was calculated by forage productivity and dry matter intake (DMI) and was the highest in May (65 head/ha). As shown in the results of this research, grazing intensity was suggested to average 39 head/ha from May to October. It is desirable that adequate grazing intensity was maintained by adjusting supplemental feed.

본 연구는 흑염소 사육을 활성화하고 경영의 효율성을 높일 수 있는 산지초지의 활용성 제고를 위한 기초자료를 제공하기 위하여 우리나라 흑염소 방목 이용 농가(20호)에 대한 실태조사를 실시하였다. 야초지로 이루어진 방목지의 경우 계절별 초지 생산성이 여름에 가장 높았으며(p<0.05), 봄철과 가을철에는 급격하게 저하되었다. 반면 목초지로 이루어진 방목지의 초지 생산성은 봄철에 가장 높은 생산성을 나타냈으며 가을철에 생산성이 다소 저하(p<0.05)되긴 하였지만, 대체적으로 계절별 생산성의 차이가 야초지 방목지에 비해 완만하여 비교적 안정적인 생산성을 나타내고 있었다. 계절별 방목지 초지의 사료가치 비교 결과 목초지의 경우 계절에 상관없이 안정적인 건물함량을 나타냈고, 야초지는 봄에서 가을로 갈수록 건물함량이 증가하는 경향을 보였다. 조단백질 함량은 목초지의 경우만 가을철에 증가하였고(p<0.05), 야초지의 경우에는 봄에 비해 여름과 가을에 감소하는 경향을 보였다(p<0.05). 목초지의 경우 야초지에 비해 상대적으로 조지방 함량은 높고 조섬유소 함량은 낮은 경향을 나타냈다. 계절에 따른 농장별 흑염소의 일당증체량은 농장별 초지 생산성 및 초지관리 기술에 따라 다양한 차이가 나타났다. 결과적으로 흑염소 사육의 활성화와 경영의 효율을 높이기 위하여 방목 야초지를 우수한 사료가치를 가지고 있을 뿐 아니라 계절별 생산성의 변화도 적은 방목 목초지로 갱신 및 조성을 해 나가야 할 필요성이 있으며, 산지초지의 효율적 이용을 위해서 초지관리 기술의 메뉴얼화와 보급으로 초지 생산성의 개선을 추구할 수 있는 방안이 필요할 것으로 사료된다.

This study was aimed to determine the biometry of genital organs, incidence of gynecological disorders and pregnancy loss in Black Bengal goat (Capra hircus). Genitalia of 118 does were collected from local abattoirs. Biometric parameters of genital organs were measured and gross and histopathological examinations were carried out for detection of abnormalities. For gravid uterus, age of the fetus was determined by measuring crown-rump length. There was no significant difference in the length, width and weight of right and left ovaries (P>0.05). However, the number of follicles between left (5.3 ± 2.3) and right ovaries (7.4 ± 2.7) varied significantly (P<0.05). The mean length of right fallopian tube and uterine horn were not varied with those of left fallopian tube and uterine horn. The length of uterine body, cervix and vagina were 1.3 ± 0.1 cm, 3.3 ± 0.5 cm and 6.8 ± 1.3 cm, respectively. Overall, 29 (24.6%) genitalia had abnormalities. Fifteen genitalia (12.7%) had ovarian abnormalities including ovaro-bursal adhesions (6.8%), parovarian cyst (5.1%) and follicular cyst (0.9%). Uterine abnormalities were found in 12 genitalia (10.2%) and predominant uterine lesion was endometritis (6.8%) followed by adenomyosis (1.7%), hemorrhagic lesion on endometrial surface (0.9%) and cyst in broad ligament (0.9%). In addition, cyst in fallopian tube (0.9%) and vagina (0.9%) were recorded. The proportion of slaughtered pregnant goats was 15.3% (18/118). The pregnancy wastage was highest in the first month (50.0%) followed by second (33.3%) and third (16.7%) month. It can be concluded that ovaro-bursal adhesions, parovarian cyst and endometritis are the gynecological disorders of major concern in Black Bengal goat.

In vitro maturation and activation of oocytes are primary steps towards biotechnological manipulation in embryology. The objectives of the present study were to determine the oocyte recovery rate per ovary, in vitro maturation rates of oocytes and rates of parthenogenetically activation of matured oocytes in Black Bengal goats. All visible follicles were aspirated to recover follicular fluid from individual ovaries (number of ovaries = 456). The immature cumulus oocyte complexes (COCs; n = 1289) were cultured in tissue culture medium (TCM)-199 supplemented with 10% (v/v) fetal bovine serum (FBS) for 27 hours at with 5% in humidified air. The matured oocytes (n = 248) were activated with 5 ionomycin for 5 minutes followed by treatment with 2 mM 6-dimethylaminopurine (6-DMAP) for 4 hours. After activation, oocytes were cultured for another 14 hours in TCM-199 supplemented with bovine serum albumin (BSA) at with 5% in humidified air. The pronucleus formation in activated oocytes was determined by staining with 1% orcein (whole mount technique). Matured oocytes (n = 176) without activation stimuli were used as control. The mean number of oocytes recovered per ovary was . The proportion of oocytes matured in vitro, confirmed by the presence of first polar body, was . Parthenogenetic activation, evidenced by formation of pronucleus, occurred in of matured oocytes. No pronucleus formation was observed in control oocytes. In conclusion, a combination of ionomycin and 6-DMAP induces activation in one third of Black Bengal goats' oocytes.

본 연구는 조사료원으로 청보리 사일리지 급여시 농후사료 급여수준이 육성기 흑염소의 가소화 영양소 섭취량, 영양소 이용율 및 질소축적율에 미치는 영향을 조사하였다. 시험구는 4처리구 (볏짚+농후사료 체중의 2.0%, 청보리사일리지 + 농후사료 체중의 2.0%, 체중의 1.5%, 체중의 1.0%)로 나누어 처리구당 3두씩 라틴방각법으로 시험을 실시하였다. 시험기간 동안의 1일 두당 가소화 조단백질 섭취량은 대조구, 청보리 사일리지에 농후사료를 각각 체중의

본 연구는 조사료원으로 청보리 사일리지 급여시 농후사료 급여수준이 육성기 흑염소의 가소화 영양소 섭취량, 영양소 이용율 및 질소 축적율에 미치는 영향을 조사하였다. 시험구는 4처리구 (볏짚+농후사료 체중의 2.0%, 청보리 사일리지 + 농후사료 체중의 2.0%, 체중의 1.5%, 체중의 1.0%)로 나누어 처리구당 3두씩 라틴방각법으로 시험을 실시하였다. 시험기간 동안의 1일 두당 가소화 조단백질 섭취량은 대조구, 청보리 사일리지에 농후사료를 각각 체중의 2.0과 1.5% 급여구가 농후사료를 1.0% 급여구 보다 유의하게 높았다(p<0.05). 가소화 NFC 섭취량은 청보리 사일리지와 농후사료를 체중의 2.0% 급여구가 가장 높았고, 농후사료를 1.0% 급여구가 가장 낮았다 (p<0.05). 일당 증체량은 청보리 사일리지+농후사료 2.0%, 청보리 사알리지+농후사료 1.5%, 볏짚+농후사료 2.0%, 청보리 사일리지+농후사료 1.0%, 순으로 유의하게 낮아졌다(p< 0.05). 건물소화율은 청보리 사알리지와 농후사료를 1.5% 급여구가 대조구 보다 유의하게 높게 나타났다(p<0.05). 질소축적량은 대조구와 청보리 사일리지에 농후사료를 각각 체중의 2.0과 1.5% 급여구가 농후 사료 1.0% 급여구보다 유의하게 높았다(p<0.05). 이러한 결과를 종합적으로 고려할 때 청보리 사일리지를 조사료원으로 급여시 육성기 흑염소의 생산성과 효율적인 영양소 이용을 위한 농후사료 급여수준은 1.5%가 적정한 것으로 사료된다.