본 연구는 Acacia baileyana와 A. dealbata 종자의 휴면타 파와 수분흡수를 토대로 발아특성을 구명하여 대량으로 번식 시킬 수 있는 체계를 확립하고자 수행하였다. 휴면을 타파시키기 위하여 nicking 처리 및 열탕 처리를 하였다.또한 각 처리 별로 염료추적을 통하여 수분흡수 부위의 양상을 알아보았다. A. baileyana의 경우, nicking 처리는 무처리구(20.0%)에 비해 85.3%의 가장 높은 발아율을 보였으며, 80℃의 60분 열 탕 처리도 무처리구(18.0%)에 비해 76.0%의 높은 발아율을 보였다. Nicking 처리 후 절단면에만 vaseline 을 도포한 처리 와절단면을 포함한 종피 전체에 vaseline 을 도포한 처리 모두에서는 전혀 발아가 이루어지지 않았으며 열탕 처리 전이나 후에 종피 전체에 vaseline 을 도포한 처리에서도 전혀 발아하지 않았다. 높은 발아율을 보인 nicking 처리와 80℃의 60분 열탕 처리에서 수분흡수율이 높게 나타났고 흡수 초기에 급격하게 수분이 흡수되는 경향을 보였다. A. dealbata에 있어서 가장 높은 발아율은 80℃의 5분 열탕 처리에서 100%로 나타났으며 nicking 처리에서도 96.5%의 높은 발아율을 보였다. 대조구, nicking 처리 후 절단면과 종피 전체에 vaseline 을 도포한 처리 등의 종자는 발아하지 않았다.

산형과 식물의 종자는 형태생리학적 휴면을 하고 있는 것으로 알려져 있다. 형태생리학적 휴면은 배가 미숙하고 생리적으로 휴면상태인 종자휴면중의 하나이다. 본 연구는 산형과의 세 종, 갯기름나물, 고본, 갯방풍 종자의 휴면타파와 발아에 미치는 온도, 광, 화학물질의 효과에 대하여 조사하였으며 그 결 과는 다음과 같다. 1. 본 시험에 사용된 종자의 크기는 길이 5.57 - 9.7 mm, 폭 3 - 7 mm 범위였고, 천립중은 갯기름나물 0.50 g, 고본 0.21 g, 갯방풍 17.53 g 이었다. 2. 예냉, 광, GA3, KNO3, 그리고 온도조건을 달리하여 전처리를 실시한 결과, 갯기름나물, 고본, 갯방풍의 최대 발아율은 각각 62.6%, 43.3%, 36.4%였다. 3. 갯기름나물은 4oC에서 7일 동안 예냉처리 하였을때 휴면 타파와 발아에 가장 효과적이었고 GA3의 저온 대체효과는 없었으며 20oC 항온조건에서 발아에 적합하였다. 4. 고본의 경우도 갯기름나물과 유사하게 4oC에서 7일간 예냉처리 하였을때 효과적이었으며 GA3와 KNO3 처리는 휴면타파에 효과가 없었다. 갯기름나물과 고본 두 종은 휴면타파와 발아를 위해 저온을 필요로 하였다. 5. 갯방풍은 GA3 200 mgL−1 처리가 발아에 효과적이었고 15/20oC(16/8h) 변온조건과 20oC 암조건에서 비교적 잘 발아하였다.

본 연구는 기내에서 재생된 오리엔탈나리 ‘Casa Blanca’ 와 ‘Siberia’ 소인경의 새로운 휴면 타파방법을 모색하고 자 생장조절물질을 첨가한 온탕처리 조건을 검토하였다. 오리엔탈나리 ‘Casa Blanca’와 ‘Siberia’ 두 품종 모두 30oC 증류수, 120분 온탕처리에서 맹아소요일수는 단축되 었으나 맹아율이 낮았다. GA4+7 100mg·L−1용액을 35oC 에서 1시간 온탕 처리에 의해서 ‘Casa Blanca’와 ‘Siberia’의 맹아율은 각각 76.7%으로 크게 촉진되었고 정식 2년차의 생장과 구근 비대가 촉진되었으며, 휴면에 따른 호흡정도를 반영하는 미토콘드리아 단백질 함량과 fumarase 활성도 높아 휴면타파의 생리적 원인도 구명 하였다. 생장조절물질(GA4+7)을 첨가한 온탕처리에 의해 기내에서 재생된 오리엔탈나리 소인경의 휴면을 효과 적으로 타파할 수 있었다.

포도 시설재배시 년 2기작 재배를 하려면 가장 문제가 되는 것이 여름의 휴면타파에 의한 2차 생장의 유도이다. 본 연구에서는 근권환경조절에 의하여 토양수분조절과 휴면타파제 처리에 의하여 발아율을 높이기 위한 연구를 수행하였다. 근권수분조절에 의하여 신초의 등숙을 7, 8월에 유도할 수 있었다. 근권환경조절에 의하여 1차 생장은 일반 시설재배의 신초생육과 차이가 없었다. 2기작 재배를 위한 휴면타파 처리제는 시아나미드화합물에 메리트청을 혼합한 구가 75% 이상의 높은 발아율을 나타내었다. 신초 등숙 유도를 위한 수분중단구가 수분공급구 보다 발아율이 높았다. 결실 신초율은 처리간 타이가 없었으나 신초발아 시기가 균일하지 않았다

Liatris spicata 구경(球莖)의 휴면도입기(休眠導入期) 및 휴면각성기(休眠覺醒期)를 조사(調査)함과 동시(同時)에 GA처리(處理)에 의(依)한 휴면타파효과(休眠打破效果)를 조사(調査)한 결과(結果)는 다음과 같다. 1. 맹아율(萌芽率), shooting율(率) 및 발근율(發根率)은 8월(月) 30일(日) 이후(以後) 서서히 저하(低下)되어 11월(月) 14일경(日頃) 가장 낮은 경향(傾向)을 보이다가 이후(以後) 서서히 증가(增加)하여 1월(月) 29일(日) 이후(以後)에는 모두 100%로써 매우 양호(良好)하였다. 2. 출아소요일수(出芽所要日數)에 있어서도 11월(月) 14일(日) 무처리구(無處理區)에 있어서 10.1일(日)로써 가장 길었으며 1월(月) 29일(日) 이후(以後)에는 급격(急激)히 단축(短縮)되었다. 3. 맹아(萌芽) 및 shooting 상태(狀態)를 봐서 대구지방(大邱地方)의 노지(露地)에서 Liatris spicata 구경(球莖)의 휴면도입기(休眠導入期)는 9월(月) 하순경(下旬頃), 휴면기(休眠期)는 11월(月) 중순경(中旬頃), 휴면각성기(休眠覺醒期)는 1월(月) 하(下)~2월(月) 초순경(初旬頃)이라고 사료(思料)된다. 4. 구경(球莖)의 휴면타파효과(休眠打破效果)는 GA 200ppm 1시간(時間) 침지처리(浸漬處理)로 효과(效果)가 인정(認定)되었으며 특히(特)히 휴면(休眠)이 깊을수록 그 효과(效果)는 더욱 증가(增加)되는 경향(傾向)을 보였다.

This study was conducted to determine the number of days required to break a plant’s dormancy and promote subsequent crop growth in new varieties of Gomchwi through the 4℃ treatment. Three new varieties of Gomchwi namely, ‘Sammany’, ‘Gommany’, and ‘Damogy’ were observed in this study. The rate of leaf emergence of ‘Sammany’ after 15-day of 4℃ treatment was 100%, while ‘Gommany’, and ‘Damogy’ took 20-days and 10-days, respectively to reach to 97.9% rate of leaf emergence. After 10-days of 4℃ treatment, ‘Damogy’ grew faster than the other varieties. and Harvest time for ‘Damogy’ was on January 18th, after 5-days of 4℃ treatment and yield was observed to be the highest at 15-days of 4℃ treatment. ‘Sammany’ was next with a minimum of 10-days of 4℃ treatment, although 15-days is more preferred for better harvest. ‘Gommany’ on the other hand, did not grow enough for harvest by January 18th, and its harvest time was delayed to January 31st. It needed a minimum of 15-days and preferentially 20-days of 4℃ treatment to grow normally and be ready for harvest. The plant height, leaf length and leaf petiole length appeared to grow better by extending duration of the 4℃ treatment. The number of leaves of ‘Sammany’ and ‘Gommany’ varieties was three leaves for the 5-days treatment which may be due to the incomplete breaking of dormancy. Regarding the yield per plant, ‘Sammany’ yielded 112.3 grams (g) in 15-days treatment, and ‘Gommany’ yielded 106.5 g in 25-days treatment. In the case of ‘Damogy’, it yielded 123.5 g and 183 g in the 10-days and 25-days treatment respectively. It is concluded that ‘Damogy’, ‘Sammany’ and ‘Gommany requires 10, 15, and 20 days of 4℃ treatment to break the plant’s dormancy and promote better plant growth.

This study was investigated the effect of immature rhizome production according to harvest times-based treatment method for seed production of Gastrodia elata. The results revealed that when the tuber weight of G. elata harvested in spring (GEHS) was ≥ 100 g, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was 90.9%–94.8%, 3.1%–5.4%, and 10.1%–15.3%, respectively. When G. elata harvested in fall (GEHF) was treated at a low temperature for 4 weeks or more, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was 70.4%–87.6%, 2.2%–2.6%, 8.7%–9.5%, respectively. Therefore, to produce seeds and immature rhizomes, GEHS must have tubers of more than 100 g, whereas GEHF requires breaking dormancy by low-temperature treatment for 4 weeks or more. Compared with those of GEHS, the rate of artificial fertilization, protocorm formation, and immature rhizome formation was lower in GEHF; however, it was higher than those in the natural germination state. Thus, it can be expected that G. elata can be produced throughout the year by ensuring that the seeds and immature rhizomes of G. elata are produced using a constant tuber weight and by breaking dormancy with low temperature treatment.

Background: Developing new ginseng cultivars is a significant time-consuming process owing to the three years of growth required for ginseng to flower. To shorten the ginseng breeding process, it is necessary to establish rapid progression through each generation. In this study, we examined it was possible to rapidly break ginseng seed dormancy using gibberellic acid (GA3) treatment and alternating temperature. Methods and Results: Seeds were obtained from local variety. Seeds were treated with either GA3 at a concentration of 100 ㎎/ℓ, constant temperature (−2℃ and 2℃), alternating temperature (2℃ followed by −2℃, followed by 2℃) or a combination GA3 and temperature treatment. Following experimental treatment, seeds were sown into trays and placed in a greenhouse. Low germination rates were observed in seeds that did not receive GA3 treatment, which were similar following 2℃ and −2℃ constant temperature treatment. Germination rates increased in proportion to GA3 and more so when combined with alternating temperature treatment. In additon, stem and leaf lengths of the resulting ginseng plants were increased following GA3 treatment, although no synergistic effect was observed with alternating temperature treatment. Conclusions: These results suggest that a combination GA3 and alternating temperature treatment enhances ginseng seed germination, which can contribute to shortening the time required to progress through a single ginseng generation for breeding.

This study was carried out to develop an effective seed propagation method for Thalictrum rochebrunianum var. grandisepalum (H. Lev.) Nakai by analyzing seed dormancy types and germination characteristics. Seeds were collected between September to October at Gangwon province, and well-selected seeds were used while being dry-stored at 4±1℃. The seed size ranged 4.52 × 1.58 ㎜ and the weight of thousand seeds were 1,603.5 ± 0.02 ㎎. The moisture content was 7.2%. Seeds were achene type, and morphology characters showed an elliptical shape and rough texture, and light brown in color. Moist-chilling treatment was conducted for dormancy breaking because the seeds had an undeveloped embryo of liner type. The embryo had developed during a moist-chilling period, constantly, and fully developed in 10 weeks. Consequently, it seemed to be non-deep complex or intermediate complex type of morphophysiological dormancy, and embryo dormancy was broken by wet-chilling for 10 weeks. After 10 weeks of wet-chilling treatment, seed germination began. Germination percentage was higher in dark condition raher than light condition and recorded the maximum at 25℃ in the dark (16.3%). A pre-soaking treatment with a combined plant growth hormones promoted germination and shortened T50. Specifically, seed germination of 84.5% was achieved by pre-soaking of seeds with a combined solution of 500 ㎎/L GA3 and 10 ㎎/L kinetin for 24 h after a wet-chilling treatment for 10 weeks. Thus the effect of plant growth hormones coupled with chilling temperature on seed breaking dormancy provide asubsequent growth of seedlings for successful plantation.

Background : When ginseng seeds were gathered, the seeds were unripe. To grow immature embryo definitely, special treatment called dehiscence must be performed. Even though dehiscence is completed, most ginseng seeds are on enforced dormancy. The breaking seed dormancy is generally achieved using cold treatment. Also it is reported that gibberellin treatment can replace the treatment. It is very time consuming process in order to develop new ginseng cultivar because ginseng flowers after 3 years of growth. To shorten the ginseng breeding period, it is necessary to establish fast generation progress. Therefore, this study examined the possibility of breaking seed dormancy of ginseng using GA3 treatment and alternating temperature. Methods and Results : Seeds were obtained from local variety fruit which is not inbred. Gibberellin of 100 ppm was treated at seeds for 24 hours. Fixed cold condition was treated on both –2℃ and 2℃. Alternating cold condition was treated on 2℃ and then –2℃, finally 2℃. Fixed and alternating temperature was continued for 15, 30, 45, 60, 90 days that 15 days of alternating temperature is first 2℃ for 5days and then -2℃ for 5days, finally 2℃ for 5days. The other treatment periods such as 30, 45, 60, 90 days mean 10, 15, 20, 30 days respectively. Each of 48 seeds were sowed on tray in greenhouse at 3 replication. Experimental plot was completely randomized. Conclusion : Seeds untreated with GA3 were germinated little and there is no difference between 2℃ and –2℃. Alternating temperature until 60days made no difference with fixed temperature but germination rate increased up to 70.8% when seeds were treated for 90days. Germination of seeds treated with GA3 is much higher than untreated seeds especially combined with alternating temperature.

Lespedeza species are mainly used for wildlife food and cover and for erosion control. The germination of these species can be enhanced after a fire occurrence in forest, which is known as fire-activated seeds to germinate. While the heat treatment could break seed dormancy of Lespedeza, its germination rate was quite low. We investigated that chemical scarification could promote germination of L. tomentosa. Seeds were soaked in 100% sulfuric acid (H2SO4) for 0, 1, 3, 6, 12, 24, 48, 96, 192, and 384 min, and then washed in distilled water for 24 h. Very few seeds were germinated in control (H2SO4 for 0 min). More than 90% of seeds were germinated in H2SO4 for 24, 48, and 92 min. However, some damage was observed in roots and cotyledons of seedling dipped in H2SO4 for a long time. To search the optimal soaking time in H2SO4 without defects, seeds scarified in H2SO4 for 30, 60, 90, 120, 150, 180, and 300 min were sown the commercial soil medium. Seeds treated with H2SO4 for 90 min and 150 min emerged by about 92% and 84%, respectively. Therefore, H2SO4 treatment could break the seed dormancy of Lespedeza species, and especially in case of L. tomentosa the optimal treatment time in sulfuric acid was one to two hours. Germination of L. tomentosa began promptly following the scarification and was completed within about one month, indicating that seeds has no physiological dormancy, just has physical dormancy.

Background : Considerable time and effort is required to develop new Panax ginseng varieties. Ginseng breeders have been developing techniques to shorten the breeding cycle to resolve this problem. In this study, we investigated the effects of adding GA3 and alternating temperature (AT, 2℃→ −2℃ → 2℃) on breaking bud dormancy in the varieties (Chungsun and Sunun) of ginseng root. Methods and Results : The GA3 soaking treatment and AT were applied to one year old roots, which greatly accelerated the emergence of new buds. In one year old roots, new buds emerged from the 4th day post transplanting and after breaking dormancy with GA3 and AT treatments. The emergence of new buds was completed within two weeks. The rate of bud emergence for Chungsun was 60% - 98% over 15 - 60 days after the AT and GA3 treatments. The emergence rate of Sunun was 46% - 92%. Normal growth of the ginseng seedling was observed in spite of the early breaking of bud dormancy by combined GA3 and AT treatments. Conclusions : GA3 and AT treatments shortened the dormancy period and facilitated the stable emergence of ginseng seedlings. However, some plants suffered deformities and early sprouting owing to the combined GA3 and AT treatments. Early sprouting was free from dormancy after leaf fall from the of aerial part of the plant.