This study demonstrated that hyaluronic acid (HA) accelerated peripheral nerve regeneration after crush injury to the common peroneal nerve in an experimental rabbit model. Ten male New Zealand White rabbits, weighing 1.8 to 2.0 kg, were used in this study. After creating the nerve crush model in every right leg, rabbits were divided into two groups. Animals in group A received application of HA into the area surrounding the crushed nerve, and group B was the sham control. Electrophysiological assessment was performed every week. After 10 weeks, nerve histological examination, muscle weight and muscle histology were used to evaluate regeneration of the injured common peroneal nerve. No differences in electrophysiological assessment were observed between the two groups. In peripheral nerve histology, myelinated nerve fibers were observed more frequently and less connective tissue was observed in the crushed nerve of group A. Fewer muscle degenerative changes, such as fibrosis, atrophy, and centrally located myonuclei, were detected in group A than in group B. In conclusion, HA could become a potential neuroprotective agent for improvement of peripheral nerve regeneration after crush injury.

본 연구는 가지(Solanum melongena L.) 과실의 수확 후 품질보전을 위한 기술개발을 목적으로 MAP 저장 중 내 외부 저온장해의 발생에 미치는 1-MCP 및 이산화탄소의 영향을 구명하기 위하여 실시하였다. 가지의 저온장해 증상은 1-MCP 처리 후 4 및 8℃ 저장구에서 과실의 저온저장기간 경과에 따른 장해의 진전을 현저히 늦추었고 1.0μL·L-1 처리구는 8℃ 저장구에서 10일간 저온장해가 전혀 발생하지 않았고 4℃ 저장구에서는 현저히 장해의 진전을 늦추었으나 1℃ 저장 처리구에서의 장해경감 효과는 보이지 않았다. 또한 1-MCP 0.1~1.0μL·L-1 처리구의 경우, 4 및 8℃에서 15일간 저온저장 후 무처리구에 비해 감모율이 낮고 경도가 높게 유지되는 것으로 조사되어 품질적 측면에서 긍정적 효과를 보였다. 드라이아이스(5g/3과)를 이용하여 MAP 가지에 이산화탄소를 처리하였던 경우, 처리 3일 후에 저온장해가 급격히 증가하였고 1-MCP 처리는 장해의 진전을 막지 못하는 것으로 조사되었다. 반면, 가지 과실의 MAP 저장 시 이산화탄소 흡수제(5g/3과)의 단용처리 및 1.0μL·L-1 농도의 1-MCP를 복합처리 하였던 경우, 4 MAP 저장 중 과실의 외부장해는 6일간 전혀 발생하지 않았고 내부장해는 복합처리의 경우 15일간 방지할 수 있었다. 종합적으로 본 연구 결과, 가지 과실에 대한 1-MCP 및 이산화탄소흡수제 처리는 가지 과실의 품질 악화를 지연시키며 유통기간 연장을 위한 저장온도의 범위를 넓혀주는데 실용성이 높다고 판단된다.

Several vertebrate species are able to epimorphically regenerate tissue of appendages or whole appendages such as fingertips, limbs, fins, tails, antlers, and ear tissue via the formation of a blastema of proliferating cells. For structure such as mammalian ear tissue and fingertips and antlers, the origin of the cells for regeneration is uncertain, but in others, such as fish fin regeneration and amphibian limb and tail regeneration, studies revealed that the blastema is formed by the dedifferentiation of mature cells local to the region of injury. Moreover, regeneration requires specification of the identity of new tissues to be made either in lower or higher vertebrates. Whether this process relies only on intrinsic regulative properties of regenerating tissues or whether wound signaling provides input into tissue repatterning is not known. In this review, authors have made efforts to put emphasis on signaling events, importance of polarity during regeneration and put forth how the limitations of regeneration could be overcome in higher vertebrates such as animals and humans.

 ,  , This study was conducted to develop economic injury level (EIL) of onion thrips, Thrips tabaci, on welsh onion (Allium fistulosum L. var) in the early transplanting stage. The changes of welsh onion biomass, yield loss, and T. tabaci density were investigated according to the inoculation periods of T. tabaci. In the early transplanting stage of welsh onion, the yield loss (%) increased with increasing inoculation periods: 17.0, 53.3, 38.4, and 80.8% yield loss in 5, 10, 15, and 20 d inoculation periods, respectively. The relationship between Cumulative Insect Days (CID) of T. tabaci and yield loss (%) of welsh onion was well described by a nonlinear logistic equation. Using the estimated equation, EIL of T. tabaci on welsh onion was estimated to 30 CID per plant based on the yield loss 12% (an empirical gain threshold 5% + marketable rate 93% of welsh onion). ET was calculated to 24 CID, which corresponds to 80% of EIL. Until a more defined EIL-model is developed, the present results should be useful for T. tabaci management in early growth stage of welsh onion. The effect of T. tabaci attack on the yield of welsh onion in late growing season (120 days after transplanting) was also examined. The yield of welsh onion increased at a low population density of T. tabaci and decreased at higher densities, showing a typical over-compensatory response.

목적 : 컴퓨터에 기초한 인지·지각 검사 도구인 CPAD를 이용하여 뇌손상 환자에서 합격군과 경계선군, 불합격군의 특성을 알아보고 안전 운전을 위해 필요한 기준을 제시하는데 기초 자료로 삼고자 한다. 연구방법 : 국립재활원 장애인 운전 평가 클리닉에 의뢰된 뇌병변 환자 187명을 대상으로 하여 운전경력 및 사고경험, 기저질환 유무, 시야 검사, 편무시, 신체 검진상 건측 및 환측에 있어서 근력, 구축, 경직, 뇌졸중 운동회복단계(brunnstrom stage), 감각 및 균형유지 능력과 자립 보행 가능과 보조기 사용 여부를 포함한 계단 오르내리기 시행가능 유무 및 CPAD를 이용한 인지·지각검사가 시행되었고 결측값을 제외하고 자료를 분석하였다. 결과 : 187명의 참가자 중에서 65명(34.8%)은 CPAD에서 통과하였고 41명(21.9%)은 실패하였으며 5명(2.7%) 은 경계선이었다. CPAD를 이용하여 뇌손상 환자에서 인지기능 관련 검사 결과 34.8%의 합격률을 보였고 나이가 어리고 뇌줄중 운동회복단계(brunnstrom stage)의 점수가 높을수록 유의하게 합격률이 높았다. 결론 : 본 연구를 통해 CPAD를 이용한 뇌손상 장애인의 인지·지각능력은 운전 가능성 여부를 결정할 수 있지만 안전한 운전능력을 파악하기 위해 더 많은 평가와 지속적인 연구가 필요할 것이다.

This research was attempted to seek for a positive approach within the framework of physical therapy instead of the drug treatment in the past, with regard to the ischemic brain injury in the early stage. Accordingly, the aim of this research is to observe the change of HSP27 and HSP70, the genes that are expressed in the early stage of brain injury and to investigate the effects of needle electrode electrical stimulation(NEES), upon applying NEES after ischemia. The experimental method is to give rise to global ischemia and apply NEES to 27 SD-Pat rats with the particulars of being eight-week-old, male, around 300g, and adapted to laboratory environment for more than a week, and divide them into three groups, that is, GV20 NEES group(n=9), L14 NEES group(n=9), no applied NEES global ischemia(GI) group(n=9), and then observe their changes of HSP27 and HSP70 at the time lapse of 6, 9 hr and 12 hr after ischemia, using immunohistochemistry methods. Upon observing through the immunohistochemistry method, it was noticed that there was a significant difference between the GV20 NEES group and the L14 NEES group as for HSP27 and there were significant differences among all groups as for HSP70(p<.05). Accordingly, it is supposed that the application of NEES after the outbreak of cerebral ischemia delay the apoptosis in the early ischemic part of forebrain or protect neurons against apoptosis.

Cell transplantation therapy using adult stem cells has recently been identified as a potential treatment for spinal cord injury (SCI). But, recovery after traumatic SCI is very limited. As dogs are physiologically much more similar to human compared with other traditional mammalian models in disease presentation and clinical responses, a number of researches demonstrated canis familiaris is a suitable model for human diseases. This study investigated the effect of transplantation of canine Mesenchymal Stem Cells (cMSC) and neural-induced cMSC (nMSC) to understand how these cells improve neurological function in canine SCI model. The differentiation of cMSC into neural precursor cells was induced in dulbecco’s modified eagle’s medium supplemented with N2-supplement, dibutyryl cyclic adenosine monophosphate, and butylated hydroxyanisole. SCI was induced between T1 and T2 by surgical hemi-section in adult dogs, and then assigned to two groups according to the applied cell types (cMSC vs nMSC). Pelleted cMSC or nMSC were transplanted directly into the injured site after SCI, respectively. Analysis of motor function after transplantation was evaluated by modified Olby score. Magnetic resonance imaging (MRI), histological and immunohistichemical analysis were also performed. Functional recovery in group of cMSC was increasing gradually after transplantation and was higher than nMSC. In MRI, we could not confirm any difference between the cMSC and nMSC experimental groups. Immunohistochemically, beta3-tubuline and nestin were observed in injury site of two experimental groups with the expression level close to non-injured groups. Transplantation of mesenchymal stem cells could promote neuronal reconstruction and repair motor function in SCI. These showed mesenchymal stem cells could be a great candidate as a therapeutic tools in degeneration disease, and dogs could be used to explore human regenerative medicine as a promising animal model. This research was supported by iPET (Grants 110056032CG000), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

목적 : 본 연구는 척수손상 환자를 대상으로 RPS Form을 적용하여 작업치료에서의 임상적 평가의 효용성을 알아보고, 적용 사례를 단계적으로 설명하여 실제 사용 방안을 제시하고자 하였다. 연구방법 : 31세의 척수손상 남성 환자를 대상으로 RPS Form을 적용한 평가와 중재를 제공하였다. 또한 임상전문 가 6인과 RPS Form의 효용적 측면에 대하여 논의하였다. 결과 : RPS Form은 척수손상 환자의 신체구조 및 기능, 활동 및 참여 이외의 환경적 요소까지 효율적으로 평가할수 있었고, 논리적인 결정을 내릴 수 있어 임상에서 적용하기 유용하였다. 환자의 중재에 대한 반응, 또한 긍정적이었다. 결론 : RPS Form은 건강에 대한 ICF의 재활관리 서비스를 한 장의 평가지로 시각화하여 사용이 간편하고, 그 내용 이 분명하여 임상적 추론을 주도하기에 용이하였다. 또한 타 재활 전문영역과의 원활한 팀 접근과 함께 근거기반의 작업치료를 제공할 수 있었다. 따라서 RPS Form의 폭넓은 적용을 위해서는 임상의 각 질환별 특징을 반영한 ICF Core sets의 첨부가 필요할 것으로 사료된다.

Peripheral nerve injuries are very common in clinics and often result in severe functional deficits. The aim of this study was to evaluate the effect of treadmill running and electro-acupuncture on nerve regeneration and functional recovery of muscle activity following sciatic nerve crush injury in a rat model. A comparative study was conducted over 30 days on 60 adult male Sprague-Dawley rats grouped into sham control (C), electro-acupuncture (EA), treadmill (T), and treadmill plus electro-acupuncture (TEA). The left sciatic nerve was crushed for 30 sec using a hemostatic forceps and functional activity was evaluated with sciatic functional tests, nerve conduct velocity, muscle weight, and histology at 10, 20, and 30 days after injury. Muscle weight was significantly (P<0.05) increased between days 10 and 30 in the TEA group. In histology, the degree of damage was scored as C > TEA > T > EA, although necrosis and fibrosis of muscle was observed only in the TEA group. The EA and TEA groups showed rapid recovery with better myelinated axons on day 10. These results suggest that application of the TEA method with balanced exercise is a useful treatment option for peripheral nerve injury regeneration and muscle activity.

Economic injury levels (EILs) and economic control threshold (ET) were estimated for the Tea red spider mite, Tetranychus kanzawai Kishida(Acari, Tetranychidae) in Rubus coreanus Miquel. T. kanzawai density increased until the early-July and thereafter decreased in all plots except the non-innoculation plot where initial density of the mite were different each 0，5, 10, 20 and 40 adults per plant branch on May 7 in 2008. And the occurrence of the densities were increased higher innoculated density than different innoculation density. The yield was decreased with increasing initial mite density and thereby the rates of yield loss was increased with increasing initial mite density. And T. kanzawai occurrence density, yields and the rates of yield loss, where initial density of the mite were different each 0,2, 5, 10 and 20 adults per plant branch on May 8 in 2009 were similar tendency to 2008 year results. The relationship between initial T. kanzawai densities and the yield losses was well described by a linear regression, Y = 0.6545X + 3.0425 (R<SUP>2</SUP> = 0.93) in 2008, Y = 0.9031X + 2.0899(R<SUP>2</SUP> = 0.96) in 2009. Based on the relationship, the number of adults per plant branch(EILs) which can cause 5% loss of yield was estimated to be approximately 3.0 in 2008 and 3.2 in 2009. And the ET was estimated to be approximately 2.4 in 2008 and 2.6 in 2009. The relationship between initial T. kanzawai densities and occurrence density of mid-May considering the best spray timing against T. kanzawai was well described by a linear regression, Y = 0.471X + 2.495(R<SUP>2</SUP> = 0.95) in 2008, Y = 0.9938X + 3.1858(R<SUP>2</SUP> 二 0.96) in 2009. Based on the relationship, the number of adults per Ieaf(ET) in mid-May which can cause 5% loss of yield was estimated to be approximately 3.6 in 2008 and 5.8 in 2009.

The neck injury occupies the most of injury that happened by the rear impact car accident. This study was analyzed about influence of the neck injury in low speed rear impact and car crash accident investigation. There is no neck injury in low speed side rear impact. On the other hand, there is initial neck injury symptom of 10 % but no long-term neck injury symptom in low speed offset rear impact. It appeared that the possibility of neck injury in low speed rear impact is low. For the more study about the neck injury, it should be evaluate the effects of the car body structure, frame structure and rear crash pattern.

This study was implemented to verify the feasibility of motor function recovery and the appropriate period for therapy. The research began with spinal laminectomy of 40 white rats of Sprague-Dawley breed and induced them spinal crush injury. Following results were obtained by using the modified Tarlov test (MTT), Basso, Beattle, Bresnahan locomotor rating scale (EBB scale) and modified inclined plate test (MIPT). First, the measurement using the MTT confirm that the most severe aggravation and degeneration of functions are observed two days after induced injury, and no sign of neuromotor function recovery. Second, better scores were achieved by open-ground movement group on BBB locomotor rating scale test, and weight-bearing on inclined plate group show better performance on MIPT. Third, both BBB and MIPT scale manifested the peak of motor function recovery during 16th day after the injury and turn into gradual recovery gradient during 16th to 24th. Fourth, the control group showed functional recovery, however, the level of recovery was less significant when compared with group open-ground movement group and weight-bearing on inclined plate group. Hence, it was clearly manifested that the lumbar region of the spinal cord had shown the best performance when its functions were measured after the execution of specific physical training; therefore it indicated the possibility of learning specific task even in damaged lumbar regions. Thus it is expected to come out with better and more effective functional recovery if concentrated physical therapy was applied starting 4 days after the injury till 16 days, which is the period of the most active recovery.

Soybean seed injury was analyzed in the experiments in which the bean bug, Riptortus pedestris(Hemiptera: Alydidae), was released into screen-caged pots containing full seed stage(R6) of soybean. When the different stages of insects, from the 3rd instar nymphs to adults, were released into pots during 8 days in soybean R6 stage, soybean seeds with injury marks (B-type seeds) increased. The weight reduction ratio in B-type seeds was highest in the injury by the 5th instar nymphs, while the daily-produced number ratio of B-type seeds was small in the treatment. In the injury by the different number of adult released into pots, 4, 8, 16 adults caused a significant increase of B-type seeds, while 16 adults caused the significant increase of the deformed (C-type) seeds. In the injury by the different release period of adults, the total number of pods was not significantly different among treatments, while the total seed number harvested was significantly small in the release for 48 days. The release for 8 and 16 days caused a significant increase of B-type seeds, while the release for 48 days caused the increase of C-type seeds. The results indicated that injury of soybean R6 stage by the bean bug produced soybean seeds with distinct injury marks at relatively low density and during short term attack period, while it produced deformed seeds at high density and during long attack period.

The majority of strokes are caused by ischemia and result in brain tissue damage, leading to problems of the central nervous system including hemiparesis, dysfunction of language and consciousness, and dysfunction of perception. The purpose of this study was to investigate the effects of Poly(ADP-ribose) polymerase(PARP) on necrosis in neuronal cells that have undergone needle electrode electrical stimulation(NEES) prior to induction of ischemia. Ischemia was induced in male SD rats(body weight 300g) by occlusion of the common carotid artery for 5 min, after which the blood was reperfused. After induction of brain ischemia, NEES was applied to Zusanli(ST 36), at 12, 24 and 48 hours. Protein expression was investigated using immuno-reactive cells, which react to PARP antibodies in cerebral nerve cells, and Western blotting. The results were as follows: In the cerebral cortex, the number of PARP reactive cells after 24 hours significantly decreased(p<.05) in the NEES group compared to the GI group. PARP expression after 24 hours significantly decreased(p<.05) in the NEES group compared to the GI group. As a result, NEES showed the greatest effect on necrosis- related PARP immuno-reactive cells 24 hours after ischemia, indicating necrosis inhibition, blocking of neural cell death, and protection of neural cells. Based on the results of this study, NEES can be an effective method of treating dysfunction and improving function of neuronal cells in brain damage caused by ischemia.

This study is intended to examine the motor skill learning and treadmill exercise on motor performance and synaptic plasticity in the cerebellar injured rats by harmaline. Experiment groups were divided into four groups and assigned 15 rats to each group. GroupⅠ was a normal control group(induced by saline); GroupⅡ was a experimental control group(cerebellar injured by harmaline); GroupⅢ was a group of motor skill learning after cerebellar injured by harmaline; GroupⅣ was a group of treadmill exercise after cerebellar injured by harmaline. In motor performance test, the outcome of groupⅡ was significantly lower than the groupⅢ, Ⅳ(especially groupⅢ)(p<.001). In histological finding, the experimental groups were destroy of dendrities and nucleus of cerebellar neurons. GroupⅢ, Ⅳ were decreased in degeneration of cerebellar neurons(especially groupⅢ). In immunohistochemistric response of synaptophysin in cerebellar cortex, experimental groups were decreased than groupⅠ. GroupⅢ's expression of synaptophysin was more increased than groupⅡ, Ⅳ. In electron microscopy finding, the experimental groups were degenerated of Purkinje cell. These result suggest that improved motor performance by motor skill learning after harmaline induced is associated with dynamically altered expression of synaptophysin in cerebellar cortex and that is related with synaptic plasticity.

This study was estimated for cabbage aphid, Brevicoryne brassicae in Brassica campestris L. var. rapa (L.) Hartm. in order to institute of Economic injury levels(EILs). B. brassicae was innoculated on April 29, in differently 0, 5, 10, 20 and 40 adults per ten plant, respectively. After inoculated of B. brassicae, the density was increased until harvest ing gradually in all plots except non innoculate plot. and Higher inoculation density were increased higher than lower inoculation density. Percentage of damage leaf was higher in plots with higher initial aphid density than in plots with lower initial aphid density. And the leaf weight of commodity were decreased in higher initial aphid density. The decreasing rates of leaf weight of commodity was increased with increasing initial aphid density. The relationship between initial B. brassicae densities and the decreasing rates of leaf weight of commodity was well described by a linear regression, Y=0.8416X-3.5147, R2=0.94. Based on the relationship, the number of adults per 10 plant which can cause 5% loss of yield was estimated to be approximately 10.1. And EILs was estimated to be approximately 1.0 adults/plant in late April.

목적 : 척수손상으로 인한 지체장애인에게 보조공학 중재로써 컴퓨터접근 보조기구를 적용하고 그 효과를 알아보는 것이다. 연구방법 : 컴퓨터접근 보조기구 무료보급 사업을 통해 1명의 성인 척수손상 지체장애인에게 최적의 컴퓨터접근 보조기구를 보급, 적용하였다. 보조공학 중재 효과를 파악하기 위해 보조기구 적용 후 주관적 수행 향상 및 보조공학기기의 심리사회적 변화 척도(Psychosocial Impact of Assistive Devices Scale, PIADS)를 실시하여 결과를 분석하였다. 결과 : 대상자는 컴퓨터접근 영역의 보조공학 중재 후 컴퓨터 사용의 주요 불편함이 해결되었고, 컴퓨터 작업 수행 능력이 증가 되었다고 보고하였으며 삶의 질에서도 긍정적인 변화가 나타났다고 하였다. 결론 : 컴퓨터접근 보조기구의 적용은 척수손상 중도 지체장애인의 컴퓨터 작업 수행을 개선시키고 삶의 질을 향상시키는 것으로 나타났다. 이에 따라 컴퓨터접근 보조기구의 적합한 적용은 중증장애인의 삶의 질 향상에 큰 역할을 할 수 있을 것으로 기대한다.

This study was conducted to develop economic injury level (EIL) and economic threshold (ET) of onion thrips, Thrips tabaci on welsh onion (Allium fistulosum L. var). The changes of welsh onion biomass and T. tabacci density were investigated after introductions of T. tabacci at different densities and days: By the results of the experiment in the early welsh onion cultivation periods (30d after transplanting), the biomass of welsh onion significantly decreased with increasing the initial inoculated density of T. tabacci. The relationship between cumulative insect days (CID) and yield loss (%) of welsh onion was well described by a nonlinear logistic equation. Using the estimated equation, EIL and ET of T. tabacci on welsh onion were estimated at 24 and 20 CID per plant, respectively. By the results of the experiment in the late cultivation periods of welsh onion (about 120d after transplanting), the welsh onion biomass was also significantly different inoculation thrips densities to welsh onion. The EIL and ET were calculated as 35 and 28 thrips per welsh onion plant. Until a more elaborate EIL-model is developed, the present result may be useful for T. tabacci management during the cultivations of welsh onion.

Economic injury level was estimated for the sweetpotato whitefly, Bemisia tabaci on greenhouse tomato (Lycopersicon esculantum cultivar pinktop). In the greenhouse, seedling tomato transplanted at mid-April and inoculated B. tabaci at late-April with the densities of 0, 1, 3, 9, 27, 54, 108 and 216 per plant. Only 108 and 216 inoculation densities increased until mid-July. Total weight of fruits was not showed the differences by initial whitefly density; however, the total weight of marketable fruits decreased significantly among plots by higher initial whitefly density. The rates of yield loss increased with increasing whitefly density, resulting in 8.3, 14.0, 29.7, 29.7, 25.7, 40.4, and 69.4% reduction in each of the plots, respectively. The relationship between initial whitefly densities and yield losses was well described by a linear regression, Y= 18.09X+6026.5, R2=0.8504. Based on the relationship, the number of adults per plant which can cause 5% loss of yield was estimated to be approximately 17.