Mongolian herders rely significantly on grazing their animals, such as goats, sheep, cattle, horses, yaks, and camels, in broad rangelands throughout the year. The availability of appropriate forage, the amount of hay and forage to be kept, and whether the animals will acquire physical strength from the pasture to make it through the impending cold season are all determined by the meteorological conditions of the year. Herders' principal source of income is animals, therefore preventing mortality is a top priority. In Mongolia, meadows are a major element determining cattle live weight. However, in the summer of 2022, Mongolia faced a drought, which resulted in inadequate pastures and starved cattle. Livestock might lose weight in these situations due to a lack of supplemental feeding.

This study presents a novel method for addressing the issue of high-concentration contaminants (ammonium, phosphate, antibiotics) in leachate arising from decomposing livestock carcasses. Antibiotics, developed to eliminate microorganisms, often have low biodegradability and can persist in the ecosystem. This research proposes design elements to prevent contamination spread from carcass burial sites. The adsorbents used were low-grade charcoal (an industrial by-product), Alum-based Adsorbent (ABA), and Zeolite, a natural substance. These effectively removed the main leachate contaminants: low-grade charcoal for antibiotics (initial concentration 1.05 mg/L, removal rate 73.4%), ABA for phosphate (initial concentration 2.53 mg/L, removal rate 99.9%), and zeolite for ammonium (initial concentration 38.92 mg/L, removal rate 100.0%). The optimal mix ratio for purifying leachate is 1:1:10 of low-grade charcoal, ABA, and zeolite. The average adsorbent usage per burial site was 1,800 kg, costing KRW 2,000,000 per ton. The cost for the minimum leachate volume (about 12.4 m3) per site is KRW 2,880,000, and for the maximum volume (about 19.7 m3) is KRW 4,620,000. These findings contribute to resolving issues related to livestock carcass burial sites and suggest post-management strategies by advocating for the effective use of adsorbents in leachate purification.

This review paper aimed to comprehensively assess the ventilation methods and ventilation rates of livestock sheds, various livestock odor mitigation technologies, and the design flow rate of odor mitigation devices. The most efficient ventilation method for livestock odor control was found to be mechanical ventilation. When livestock odor is at its most severe during summer, ventilation systems are operated at the maximum ventilation rate, which is 5-25 times higher than the ventilation rate in winter. Therefore, the mitigation facilities of livestock odor must be designed while considering the maximum ventilation rate. There is a significant amount of research data on various livestock odor control technologies using various physical, chemical, biological, and complex technologies applied to livestock farms. Biofiltration and photocatalytic oxidation are considered the most promising methods due to their cost-effectiveness and simplicity. Biofiltration is effective for removing hydrophilic odors, but requires improvement for the efficient removal of hydrophobic odors and the control of accumulated excess biomass. The advantages of the photocatalytic oxidation method include its excellent hydrogen sulfide and ammonia removal rates and relatively low ozone emissions. However, it requires technology to reduce nitrous oxide emissions. Investment in installing and operating these odor mitigation technologies is only realistic for large-sized farms. Therefore, it is imperative for small and medium-sized livestock farms to develop odor mitigation technology that is inexpensive and has low installation, operation, and maintenance costs.

축산물 중 잔류허용기준이 설정되어 관리하고 있는 농약 azocyclotin, cyhexatin, fenbutatin oxide는 대표적인 유 기주석계 살비제이다. 기존 시험법은 가스크로마토그래피 를 사용하여 정량한계가 높고 분석 시 재현성이 떨어져 이에 대한 개선이 필요한 실정으로 본 연구에서는 비교적 간편하며 시간이 적게 소요되는 QuEChERS법을 활용하여 azocyclotin, cyhexatin, fenbutatin oxide의 시험법을 마련하 고자 하였다. 1% 아세트산을 함유한 아세트산에틸:아세토 니트릴(1:1) 혼합액을 이용하여 진탕 추출 후 d-SPE로 정 제하고 이를 농축 후 LC-MS/MS를 이용한 시험법을 개발 하였다. Azocyclotin, cyhexatin 및 fenbutatin oxide의 결정 계수(R2)는 0.99 이상으로 높은 직선성을 확인하였으며 정 량한계는 0.01 mg/kg으로 높은 감도를 나타내었다. 대표 축산물 5종(소, 돼지, 닭, 계란, 우유)에서 LOQ(0.01 mg/ kg), MRL(0.05 mg/kg), MRL 10배(0.5 mg/kg)의 농도에서 회수율 실험을 한 결과 평균 회수율이 76.4-115.3% 및 84.4-110.8%이었으며, 상대표준편차는 25.3% 이하로 나타 났다. 본 연구는 Codex 가이드라인(CAC/GL 40-1993, 2003) 및 ‘식품의약품안전처 식품의약품안전평가원의 식 품등 시험법 마련 표준절차에 관한 가이드라인(2016)’에 적합한 수준임을 확인하였다. 따라서 본 연구에서 확립한 시험법은 축산물 중 잔류할 수 있는 azocyclotin, cyhexatin, fenbutatin oxide의 안전관리를 위한 공정시험법으로 활용 가능할 것으로 판단된다.

This study examines the correlation between livestock odor civil petitions and the establishment of malodor control areas in Jeju Special Self-Governing Province, focusing on swine farms where numerous civil petitions regarding malodors have been received. After the designation of the malodor control areas, high odor concentrations occurred in Aewoleup and Jocheon-eup, and the odor concentration decreased in other areas. The number of civil petitions shows a consistent annual trend, with increased petitions from March, peaking during summer (July and August), and decreasing from September into winter. In Jeju-si, there were many civil petitions in Hallim-eup and Aewol-eup where there were many malodor control areas. However, in Seogwipo-si, there were also many civil petitions in Pyoseonmyeon, where there is no malodor control area. Additionally, we compared the average multiple of compound malodors and the rate of exceeding the maximum allowable emission level for compound malodors with the number of livestock malodor civil petitions to assess the actual state of malodors. The results reveal a stronger correlation between the number of civil petitions and the rate of exceeding the compound malodors allowable emission level than the average multiple for compound malodors. These findings provide valuable insights into addressing livestock odor concerns and enhancing malodor control measures in Jeju Island.

We conducted an on-site application study at the livestock cooperative fertilizer plant to compare the composting period, temperature change, moisture content, and chemical properties between livestock manure compost using sawdust as a moisture regulator with those using spent oyster mushroom substrate. The composting period, moisture content, and fertilizer composition of compost containing spent oyster mushroom substrate did not differ from that of conventional compost mixed with sawdust after the first and second fermentation and post-maturation stages, it was suitable as a material for manufacturing livestock manure compost. The spent oyster mushroom substrate also lower the production cost of livestock manure compost by replacing the more expensive sawdust. The developed technology is expected to contribute towards the utilization of by-products of the oyster mushroom harvest while simultaneously producing high quality livestock manure compost.

이 논문의 목적은 축사시설현대화 사업에 참여한 농가들이 사업 참여에 따라 생산성에 얼마나 향상을 가져왔는지를 계측하는 것이다. 분석 방법으로는 이중차분법을 사용하였다. 실험군 농가에 해당하는 사업참여농가들은 농협경제지주에서 보유한 사업참여농가들의 자료를 사용하였다. 대조군 농가에 해당하는 자료들은 통계청의 축산물생산비통계 조사를 위해 선정된 표본 농가들의 자료를 사용하였다. 이중차분법을 이용한 사업참여 의 순효과를 계측한 결과, 한우는 1등급 이상 출현율이 10.7%p 만큼 상승하였다. 젖소 농가들의 두당 착유량은 2.8% 증가하였다. 돼지 농가들의 PSY는 5.8% 증가하였다. 육계 농가들의 일당증체량은 4.5% 증가하였다. 산란계 농가들의 산란율은 5.2%p 상승한 것으로 나타났다. 이러한 결과는 선행연구들과 비교할 때 정도의 차이는 있지만 축사시설현대화 사업이 생산성에 긍정적인 영향을 미친다는 점에서 동일하다.

This review comprehensively summarizes the livestock odor reduction method by dietary manipulation, in-housing management, and manure management. The gut microbial metabolism of animals can be stimulated by low-crude protein feeding and the addition of probiotics, enzymes, plant extracts, and/or organic acids to their feed. These methods can result in reduced odor emissions from manure. For in-housing management, it is important to maintain the proper breeding density in the barn facilities, regularly remove dust and manure, and periodically clean the barn facilities. A barn using litter on the floor can reduce odor at a relatively low cost by adding adsorbents such as zeolite, biochar, etc. Although masking agent spraying can be the simplest and quickest way to control odors, it is not a fundamental odor mitigation strategy. Odor emissions can be reduced by installing covers on manure slurry storage facilities or by acidifying the manure slurry. It is necessary to install a solid-liquid separator in an enclosed facility to minimize odor emissions. Other methods for reducing odor emissions include covering manure composting plants with semi-permeable membranes or using reactor composting technology. In order to minimize odor emissions in the liquid manure composting, sufficient oxygen must be supplied during the fermentation process. Furthermore, the odor reduction effect can be achieved through the liquid manure pit recharge system which supplies matured liquid manure fertilizer to the slurry pit in the pig house.

식량 생산량은 지난 반세기 동안 세 배로 증가했다. 세계의 인구는 30억에서 70억으로 증가했고, 기대수명은 46세에서 65세로 증가했다. 전 세계 가축 생산량이 56% 증가한 것은 가축 개량 덕분이다. 2050년에 세계 인구는 98억 명으로 추정되므로, 생산량은 현재 두 배 이상 증가해야 한다. 경제 동물의 수가 증가하고 생산자의 수가 감소함에 따라, 생산자는 농장을 관리하고 통제해야 할 동물이 더 많아졌다. 농장의 사육밀도가 높으면 개체 간 질병 발생과 성장 능력 저하를 가속화할 수 있으며, 이는 제품의 질을 해칠 뿐만 아니라 동물 복지에 대한 심각한 우려를 불러일으킬 수 있다. 1980년대 후반에 기술을 통해 토양 사용을 극대화하는 동시에 환경 영향을 줄이기 위해 작물 시스템에 정밀 농업(PA)이 도입되었다. 이해해야 할 중요한 것은 PA가 효율성을 극대화하고 환경 피해를 최소화한다는 목표와 함께 동물 산업과 같은 모든 농업 생산 시스템에 적용된다는 것이다. 동물 산업에서 PA의 예로는 생산과 동물 관리의 효율성을 향상시키고, 동물 복지 및 건강에 대한 우려를 해결하며, 환경 손상과 자원 사용을 최소화하는 데 전념하는 정밀 축산 농업이 있다. 특히 널리 보급된 것은 이미지 분석, 동물 실시간 모니터링, 센서, 이미지 및 기록으로부터의 데이터 수집과 같은 고급 기술의 사용이다. 본 연구의 목적은 농장의 생산성과 관리를 향상시키면서, 동물 육종 시스템에 적용가능한 신기술을 살펴보는 것이다.

In this review paper, the sources of odor, major odor compounds, and emission characteristics from livestock farms are summarized. The main sources of odor on livestock farms are barn facilities, manure storage facilities, manure composting facilities, and wastewater treatment facilities. High concentrations of odor are emitted during the manure removal process, and livestock odor tends to be the most severe in summer. There was a remarkable difference in odor intensity depending on the farm size and the cleaning condition, and odor intensity varied greatly depending on the weather parameters such as wind direction and speed. The concentrations of ammonia and hydrogen sulfide were high among the odor compounds emitted from livestock farms, and these compounds also contributed to odor intensity. The odor intensity in poultry and swine farms was higher than in cattle farms. Information on livestock odor emission is very useful for managing livestock odor complaints and designing odor abatement technologies.

The livestock industry continues to grow around the world, but livestock odor is becoming an environmental problem that is difficult to solve. In this review paper, the current status of the domestic livestock industry, livestock odor complaints, mediation cases involving environmental disputes related to livestock odor, livestock odor management policies and standards, livestock odor sources, major odor compounds, and emission characteristics are summarized. Domestic meat supply and meat consumption per capita are increasing, and livestock farms are becoming large-scale and intensive. Livestock odor complaints increased 4.5 times over the last five years (2014-2019), and its proportion to total odor complaints was 19%-30%. Livestock facilities larger than a certain size are classified as odor emission facilities and are managed based on the Odor Prevention Act. The information presented in this paper can be used to establish strategies to promote the sustainable development of the livestock industry while resolving air quality deterioration and public health problems caused by odor emissions from livestock farms.

본 연구는 네팔의 지체 및 시각장애인의 직업재활(축산창업지원)사업을 통해 축산에 대한 지식과 소득에 얼마나 영향을 미쳤는지 분석하고 OECD DAC 평가기준을 적용하여 분석함으로써, 장애인의 빈곤퇴치에 어떠한 영향을 미쳤는지 확인하고자 하였다. 이를 위해 현지 관계자 및 대상자와의 설문자료와 모니터링 자료를 활용하여 축산창업지원이 어떠 한 결과로 나타났는가를 세 가지 측면에서 분석하였다. 먼저 훈련 및 가 축 지원에 대한 만족도를 분석하였고, 두 번째로 2018년과 2020년 시행 했던 사업의 후속 모니터링을 분석하였으며, 마지막으로 OECD DAC 평 가기준을 적용하여 분석하였다. 결과를 살펴보면, 장애인지원사업이 효과 적으로 개발도상국 장애인의 소득증대에 도움이 되었음을 확인하였고, 전문적 지식 습득 및 가축 사육으로 인해 경제적으로 빈곤에서 벗어날 수 있는 기회가 제공되었으며, 인식의 긍정적 전환까지 나타나는 모범사 례로 적합한 사업이었음을 보여주었다. 이는 개발도상국의 빈곤퇴치를 위해서 선진화된 ODA 사업인 장애인지원사업이 필요함을 시사하며, 이 를 위한 사회적 관심과 정책적 가이드라인에 대한 적극적인 방안을 고려 할 필요가 있다.

Climate change has severely impacted food security and agricultural productivity in Africa. The scarcity of rains and the severe drought affecting the Sub-Saharan and the horn of Africa have impoverished the soil, and water resources, and have caused the death of livestock in countries like Sudan, Ethiopia, Kenya, Niger, and etc. On the other hand, the usage of chemical fertilizers for soil and crop nutrients is becoming an economic and environmental burden for African farmers. Thus, the necessity to implement sustainable agricultural technics to maintain and improve soil fertility by applying natural nutrients obtained from livestock manure while livestock is fed with crop residues in a single agricultural area. Although this farming system offers environmental and economic benefits to farmers, particularly in the rural and semi-urban areas, its implementation is very low in Africa because of: 1) constant migration of herders in the search of green pasture for livestock, making the collection of manure difficult, 2) religious and traditional beliefs considering animal waste as not being pure to be applied on crops, 3) conflicts between herders and farmers over the control of water resources and arable lands, making the cohabitation of livestock and crops in one farmland unlikely, and 4) the habit of crop residues burning, which is not just harmful to the environment but also a waste of natural livestock fed. Based on the reviewed literature, the Korea-Africa Food and Agriculture Cooperation Initiative (KAFACI) plans to develop and implement an integrated crop-livestock project in KAFACI member countries with the mission 1) to diffuse the importance of using natural nutrients for improving soil fertility and enhancing crop and livestock productivity in Africa, 2) to train researchers and farmers on new technologies for manure treatments, 3) to demonstrate the benefits of using livestock manure for soil fertility, crops nutrients, while using crop residues as livestock nutrients, and 4) contribute to enhancing sustainable agriculture in Africa through the reduction of greenhouse gas by reducing livestock’s waste and crop residues burning.

The research aims to develop a rapid and easy analytical method for methoprene using liquid chromatography- tandem mass spectrometry (LC-MS/MS). A simple, highly sensitive, and specific analytical method for the determination of methoprene in livestock products (beef, pork, chicken, milk, eggs, and fat) was developed. Methoprene was effectively extracted with 1% acetic acid in acetonitrile and acetone (1:1), followed by the addition of anhydrous magnesium sulfate (MgSO4) and anhydrous sodium acetate. Subsequently, the lipids in the livestock sample were extracted by freezing them at -20oC. The extracts were cleaned using MgSO4, primary secondary amine (PSA), and octadecyl (C18), which were then centrifuged to separate the supernatant. Nitrogen gas was used to evaporate the supernatant, which was then dissolved in methanol. The matrix-matched calibration curves were constructed using 8 levels (1, 2.5, 5, 10, 25, 50, 100, 150 ng/mL) and the coefficient of determination (R2) was above 0.9964. Average recoveries spiked at three levels (0.01, 0.1, and 0.5 mg/kg), and ranged from 79.5-105.1%, with relative standard deviations (RSDs) smaller than 14.2%, as required by the Codex guideline (CODEX CAC/GL 40). This study could be useful for residue safety management in livestock products.

Three-dimensional (3D) organoids act as model systems because they mimic in vivo tissue morphology. Recent advancements in the field have demonstrated that organoids derived from various organs have assisted in understanding the underlying mechanisms of disease modeling and expanded our knowledge of organ development in vitro. Furthermore, these organoids have become a promising biomaterial in regenerative medicine for therapeutic purposes as well as in nutritional research for feed efficiency measurement in livestock. Intestinal organoids of livestock, including pigs, cattle, chickens, and horses, have been developed. These could be used to examine host-pathogen interactions, such as interaction between enteric viruses and epithelial cells, and are potential alternatives to in vivo systems. However, there are very limited studies regarding species-specific medium to cultivate and establish intestinal organoids of livestock. Species-specific medium is applied differently between species for the cultivation of intestinal organoids, and its modification is important for the maintenance of specific cell types or genes from the cellular diversity of the intestinal epithelium. In this study, we introduce the histological development of a 3D culture system and a species-specific medium for the cultivation of intestinal organoids in livestock. Finally, we discuss the importance and future perspectives of intestinal organoids in the fields of agriculture and biotechnology for various purposes.

The sampling bag is used as a storage container for odor gas samples. It is known that the substances recovery rate of odor bags decreases during storage time, and the degree of recovery varies depending on the characteristics of the gas sample and the material of the bag. This study investigated the recovery rate of VFA (ACA, PPA, BTA, VLA) in PEA bags during storage time. In addition, a model was developed to estimate the recovery rate of each substance as a function of time. Standard gas (ACA, PPA, BTA, VLA mixed) recovery rate was used for the model development. The concentration of the compound in the bag was measured by SIFT-MS at intervals of 1 to 2 hours. The recovery rate according to the storage time was calculated as the ratio to the initial concentration. The recovery rate of each substance according to the storage period (12h, 24h, 36h, 48h) was ACA (66.2%, 62.8%, 55.6%, 52.0%), PPA (77.6%, 72.1%, 63.0%, 58.1%, 86.6%), BTA (86.6%, 81.3%, 71.6%, 66.9%), VLA (94.8%, 89.0%, 76.6%, 71.7%). The recovery rate continued to decrease over the course of 48 hours of storage time. ACA, PPA, and BTA showed the greatest decrease within the initial 12 hours, which is form of exponential decrease. Therefore, we considered a 1~3 degree polynomial regression model and a 1~2 degree exponential decay model. Each developed model was evaluated by r², RMSE, MAPE, AIC, and then a model for each substance was selected. Selected models were tested with recovery rate data from swine farm odor samples. Only the ACA model exhibited a good performance (r² = 0.76).

본 조사는 국내에서 작물재배용으로 생산 및 시판되는 복합 비료와 축산분뇨 퇴비에 함유된 CO2와 CH4의 양을 개략적으로 알아보고자 실험적으로 검토하였다. 그 결과를 요약하면 다음과 같다. 복합비료의 한 포대에서 계측된 CO2의 농도는 계측한 날짜와 관계없이 평균 약 1,733.3ppm 정도로 일정하게 나타났다. 비료 한 포대당 함유된 양은 0.067kg 정도였고, 비료의 단위 무게는 3.35×10 -3 kg·kg -1 정도였다. 국내에서 농업용으로 출하된 복합비료는 평균 885,750t·yr -1 이었다. 이 양을 기준으로 복합비료 자체에 함유된 CO2의 양은 약 2.9백 만 t·yr -1 정도로 추정할 수 있었다. CH4의 경우, 측정 개시일에 한 포대에 76.8ppm(포대당 2.949×10 -3 kg, 단위 무게당 0.15× 10 -3 kg·kg -1 ) 정도 나타낸 후, 그 이후에는 측정되지 않았다. 퇴비에 함유된 CO2의 양은 구멍의 폐쇄 및 개방 여부에 관계없이 측정 일이나 측정구에 따라 계측기의 최대 측정범위인 10,000ppm을 초과하는 경우도 있었지만, 최대인 10,000ppm 이라고 가정하고 검토하였다. 따라서 최대인 10,000ppm이라고 가정하고 검토한 결과 한 포대당 함유된 양은 0.506kg 정도 였다. 퇴비 구멍의 폐쇄 및 개방 여부에 따라 CH4의 농도가 증감하는 것으로 나타났다. 퇴비 한 포대에 함유된 CH4의 양은 약 2.53kg 정도였다. 이 양을 국내에서 생산된 고형퇴비의 평균 생산량과 비교하면, 약 4.7백만 t·yr -1 이상이 될 것으로 추 정할 수 있었다. 고추재배 포장에서 배출되는 CO2의 양은 측 정 종료 때까지 평균적으로 8,040ppm 정도였다. 나지의 경우, 시간의 경과와 함께 CH4의 농도는 점차 감소하였고, 최대 1,700ppm에서 거의 제로 상태까지 약 50일이 소요되었다.

본 논문은 과학논문을 통해 30년 후인 2050년까지 가축과 동물성식품의 동향을 예측하면서 미래 동물생명산업 발전 전략으로써 ICT-기반 스마트축산 기술의 필요성을 검토하였다. 전 세계적으로 가축사육과 동물성식품 소비는 인구증가, 고령화, 농촌인구 감소, 도시화 및 소득증가에 대한 반응으로 빠르게 변화하고 있다. 기후변화는 가축 환경, 생산성과 번식효율성을 바꿀 수 있다. 가축생산은 온실가스 배출 증가, 토지 황폐화, 수질오염, 동물복지 및 인간의 건강 문제로 이어질 것이다. 이러한 문제를 해결하기 위해 동물생명산업의 다양한 측면에서 4차 산업혁명과 융합된 ICT-기반 스마트축산을 활용하여 기후변화 대응, 생산성 향상, 동물복지, 동물성식품 영양품질 개선, 동물의 질병예방을 위한 선제적인 미래 대응전략이 필요하다. 미래 동물생명산업은 지속 가능성과 생산효율성을 향상시키기 위해 자동화를 통합해야 한다. 디지털 시대에 IoT와 빅 데이터를 사용하는 지능형 정밀가축사양, ICT-기반 스 마트축산은 동물생명산업의 다양한 소스로부터 데이터를 수집, 처리 및 분석할 수 있다. 축사 내부와 외부의 환경 매개 변수를 정밀하게 원격 제어할 수 있는 디지털 시스템으로 구성되어있다. ICT-기반 스마트축산은 인터넷과 휴대폰을 통한 원격 제어를 위해 센싱 기술을 사용하여 동물의 행동복지 및 사양 관리를 모니터링 할 수 있다. 농가가 필요로 하는 광범위한 정보의 수집, 저장, 검색 및 보급에 도움이 될 수 있고 새로운 정보서비스를 제공할 수 있다.

The purpose of this study is to obtain data on the zones formed by the movement of livestock vehicles and to determine if such areas can be used to establish quarantine activities and quarantine policies for livestock epidemics. For this purpose, this study used mobile data on poultry-related livestock vehicles generated in 2019. InfoMap, a community detection method, was used for regional classification, and the results of the analysis were visualized on a map using GIS. The study results confirmed that the zone of the administrative unit can be classified based on the movement of livestock vehicles. In addition, the zones created by the vehicle movement could be seen to change depending on the purpose and timing of the operation of livestock vehicles. Some areas form relatively stable zones, such as Jeolla-do and Gyeongsang-do, while others change depending on the situation, such as Chungcheong-do, Gyeonggi-do, and Gangwon-do. Further, the zones derived for poultry differed from those derived for cattle and pigs in previous studies.

The status of probiotics is increasing worldwide and the range of application of probiotics is also being extended to the livestock industry. The use of probiotics in the swine industry aims to improve animal health and productivity by forming a healthy gut microbiota. Intensive pig breeding, a common breeding method in modern society, causes physiological stress in pigs, resulting in imbalance of the intestinal microbiota and dysfunction of the intestinal barrier. We conducted a scientific research on the properties of probiotics for multi-strain probiotics (MSP) and observed the economic benefits and efficiency of culturing MSP through a self-cultivation system in livestock. Initially, we observed alteration occurred by MSP application in the gut microbiota of pig. MSP were characterized to have resistance to digestive juices such as gastric acid and bile, followed by colonization in the target organ, the gastro-intestinal tract (GIT). In addition, MSP was also confirmed to have antibacterial ability against pathogenicity that most frequently infected in the swine industry and showed low resistance to antibiotics which means guaranteed stability when added to feed. The growth rate of probiotics in the optimized medium used in the self-cultivation system was suitable for efficiently and economically culturing and feeding pigs with high concentrations of probiotics, considering the ingredient price and the growth efficiency. We observed the significant alteration of gut microbiota of pig by application of MSP. Importantly, the MSP supplementation significantly increased the beneficial bacteria genus of Bifidobacterium that confer a health benefit to the host in pig GIT, whereas decreased the number of harmful bacteria including coliforms. In addition, MSP influenced on the uniformity of gut microbiota at both of sow and weaning pigs. Taken together, the application of MSP with high concentration of probiotics using self-cultivation system may critically improve the pig health by regulation intestinal microbiota.