Salmonella spp.는 식중독의 주요 원인균으로, 신속하고 정확한 검출 방법이 요구된다. 본 연구에서는 간소화된 direct multiplex real-time PCR 방법인 FS Finder SL키트 의 분석법 활용 가능성을 평가하고, 평판배지법과의 검출 성능을 비교하였다. 또한, real-time PCR의 검출 효율을 평 가하기 위해 FS Finder SL 키트에서 제공하는 세 가지 전 처리 방법(Method 1, 2, 3)을 비교 분석하였다. 실험 결과, 세 가지 전처리 방법을 이용한 direct multiplex real-time PCR 방법은 Salmonella spp.를 100% 검출할 수 있었으며, Ct 값 비교를 통한 통계적 분석에서도 세 방법 간 유의한 차이가 없는 것으로 나타났다(P>0.05). 반면, 선택 배지를 이용한 검출에서는 2 log CFU/g 이상으로 접종된 샘플에 서만 Salmonella spp.가 검출되었으나 real-time PCR법의 경우 0-3 log CFU/g 범위의 샘플에서 모두 검출이 가능 하였다. 또한, 실험에 사용된 세 가지 즉석섭취식품군(알 가공품, 닭가슴살 제품, 편의점 도시락)에서 자연균총의 영 향을 평가한 결과, 도시락 샘플의 일반세균수가 3.56±0.18 log CFU/g으로 가장 높았으며, 알가공품과 닭가슴살 제품 에서는 검출되지 않았다. 결론적으로 FS Finder SL 키트 를 활용한 real-time PCR 방법은 기존의 평판배지법보다 높은 검출 감도를 보였으며, 검출까지의 소요 시간을 대 폭 단축할 수 있었다. 특히, 복합적인 식품 매트릭스에서 도 신속하고 정밀한 검출이 가능함을 확인하였다. 본 연 구는 즉석섭취식품 중 Salmonella spp. 검출을 위한 효율 적인 direct multiplex real-time PCR 분석법의 적용 가능 성을 제시하며, 향후 식품안전 관리 시스템에서 활용될 수 있는 기초 자료를 제공한다.

산업 발전에 따라 도로 연장이 지속적으로 증가하면서 폐 아스팔트 발생량이 늘어나자, 국내에서는 순환골재를 의무적으로 사 용하도록 관련 규정을 마련하였다. 현장 플랜트에서 순환 아스팔트 혼합물을 생산할 때 재생첨가제를 투입해야 하는 경우, 국 토교통부 “아스팔트 콘크리트 포장 시공 지침“에서는 재생첨가제와 아스팔트를 동시에 투입하도록 명시하고 있다. 그러나 혼합 시간이 부족하거나 혼합 과정이 불량할 경우, 재생첨가제가 균일하게 분산되지 않아 혼합물 품질이 저하될 우려가 있다. 이에 본 연구에서는 기존의 건식 혼합 방식에서 발생하는 문제점을 해소하고자, 아스팔트 플랜트의 Pre-Mixer를 활용하여 아스팔트 바인더와 첨가제를 사전에 혼합하고, 혼합 시간을 달리한 시료를 추출하여 DSR(Dynamic Shear Rheometer) 장비를 통한 MSCR 시험을 수행하였다. 그 결과, 혼합시간이 증가함에 따라 Jnr 값은 증가하고, %Recovery 값은 감소하는 경향이 확인되었 으며, 혼합 시간이 바인더 특성 변화에 유의미한 영향을 미치는 것을 확인하였다. 추가적으로 t-검정을 통해 이러한 경향의 통 계적 유의성을 검증한 결과, Jnr 값은 3-5분, 7-9분, %Recovery 값은 1-3분, 3-5분, 7-9분 구간에서 p값이 0.05 이상으로 나타 나 통계적으로 유의미한 차이가 없었다.

The seismic performance of lead-rubber bearings (LRBs) is significantly affected by both the axial force and loading rate they experience. Accurate assessment of LRBs’ seismic performance, therefore, requires realistic simulation of these forces and rates, as well as of the response of the isolated structure during seismic events. This study conducted a series of real-time hybrid simulations (RTHS) to evaluate the seismic behavior of LRBs in such conditions. The simulations focused on a two-span continuous bridge isolated by LRBs atop the central pier, exposed to horizontal and vertical ground motions. In the RTHS framework, the LRBs were physically tested in the laboratory, while the remainder of the bridge was numerically modeled. Findings from these simulations indicated that the vertical ground motion had a minimal effect on the lateral response of the bridge when isolated by LRBs.

This study proposes a method to evaluate the publicity of real-time, demand-responsive, autonomous public-transportation systems. By analyzing real-time data collected based on publicity evaluation indicators suggested in previous research studies, this study seeks to establish a system that objectively assesses the publicity of public transportation. Thus, the introduction of autonomous public transportation systems is expected to contribute to solving problems in underserved transportation areas and enable more sophisticated public transportation operations. We reviewed evaluation indicators proposed in previous studies. Based on this review, publicity evaluation indicators were derived and specific criteria were selected to assess systematically the publicity of autonomous public transportation. An AHP analysis was conducted to assess the relative importance of each indicator by analyzing the importance of the selected indicators. Additionally, to score the indicators, minimum and maximum target values were established, and a method for assigning scores to each indicator was examined. The most important factor in the publicity evaluation of autonomous demand-responsive transport (DRT) was the “success rate of allocation to weak public transportation service areas,” with a significance level p of 0.204. This was analyzed as a key evaluation criterion because of the importance of service provision in areas with low-public-transportation accessibility. Subsequently, “Accessing distance to a virtual station” (p = 0.145) was evaluated as an important factor representing the convenience of the service. “Waiting time after allocation” (p = 0.134) also appeared as an important evaluation factor, as reducing waiting time considerably affected service quality. Conversely, “compliance rate of velocity” yielded the lowest significance (p = 0.017), as speed compliance was typically guaranteed owing to autonomous driving technology. This study proposed a specific evaluation method based on publicity indicators to provide a strategic direction for improving services and enhancing the publicity of autonomous DRT systems. These results can serve as a foundational resource for improving transportation services in underserved areas and for enhancing the overall quality of public transportation services. However, the study’s limitation was its inability to use real-time autonomous public transportation data, relying instead on I-MoD data from Incheon. This limitation constrained the ability to establish universal benchmarks because data from various municipalities were not included. Future research should collect and analyze data from diverse regions to establish more reliable evaluation indicators.

Water utilities are making various efforts to reduce water losses from water networks, and an essential part of them is to recognize the moment when a pipe burst occurs during operation quickly. Several physics-based methods and data-driven analysis are applied using real-time flow and pressure data measured through a SCADA system or smart meters, and methodologies based on machining learning are currently widely studied. Water utilities should apply various approaches together to increase pipe burst detection. The most intuitive and explainable water balance method and its procedure were presented in this study, and the applicability and detection performance were evaluated by applying this approach to water supply pipelines. Based on these results, water utilities can establish a mass balance-based pipe burst detection system, give a guideline for installing new flow meters, and set the detection parameters with expected performance. The performance of the water balance analysis method is affected by the water network operation conditions, the characteristics of the installed flow meter, and event data, so there is a limit to the general use of the results in all sites. Therefore, water utilities should accumulate experience by applying the water balance method in more fields.

Decommissioning plan of nuclear facilities require the radiological characterizations and the establishment of a decommissioning process that can ensure the safety and efficiency of the decommissioning workers. By utilizing the rapidly developed ICT technology, we have developed a technology that can acquire, analyze, and deliver information from the decommissioning work area to ensure the safety of decommissioning workers, optimize the decommissioning process, and actively respond to various decommissioning situations. The established a surveillance system that monitors nuclide inventory and radiation dose distribution at dismantling work area in real time and wireless transmits data for evaluation. Developed an evaluation program based on an evaluation model for optimizing the dismantling process by linking real-time measurement information. We developed a technology that can detect the location of dismantling workers in real time using stereovision cameras and artificial intelligence technology. The developed technology can be used for safety evaluation of dismantling workers and process optimization evaluation by linking the radionuclides inventory and dose distribution in dismantling work space of decommissioning nuclear power plant in the future.

Timely detection of nuclear activity is important for the management and supervision of nuclear materials, and inspections on North Korea as a method of safety measures for this monitoring system seem to be a not far future in the rapidly changing North Korea relationship. However, a simpler and more reliable monitoring device is required since the inspection period is limited and the possibility of revisiting is unclear. The seal is a simple but easily used security device for monitoring forgery and falsification in the IAEA. IAEA presents matters related to 1) project engineering, 2) remote monitoring, and 3) seal development as the three major tasks of the Department of safeguards and Division of Technical Support. The importance of development, modernization, and application of new seal devices is emphasized, and advanced sealing and identification system development is in progress at many research institutes such as JRC, ISCN, and JAEA. Since the existing seal devices used by IAEA and KINAC can only be confirmed through on-site inspections for damage, it is difficult to respond immediately in the event of similar situations such as theft of nuclear materials and loss of continuity of knowledge. Unlike facilities that comply with the requirements for safety measures, such as domestic nuclear facilities, in the case of facilities subject to denuclearization, it is very likely that various hazardous environments will exist that make it difficult to apply safety measures. Hence, a real-time seal device has developed through prior research due to the high possibility of situations in which Continuity of Knowledge (COK) is not maintained, such as damage, malfunction, and power loss of sealing and monitoring equipment. Through previous studies, the real-time seal device was loaded with server-based operating software and improved its performance by utilizing feedback from real users (KINAC) after use. In this study, the effectiveness of the previously developed sealing system was verified through performance evaluation, and the authentication of the equipment was secured through environmental tests.

It is essential to determine a proper earthquake time history as a seismic load in a seismic design for a critical structure. In the code, a seismic load should satisfy a design response spectrum and include the characteristic of a target fault. The characteristic of a fault can be represented by a definition of a type of possible earthquake time history shape that occurred in a target fault. In this paper, the pseudo-basis function is proposed to be used to construct a specific type of earthquake, including the characteristic of a target fault. The pseudo-basis function is derived from analyzing the earthquake time history of specific fault harmonic wavelet transform. To show the feasibility of this method, the proposed method was applied to the faults causing the Gyeong-Ju ML5.8 and Pohang ML5.3 earthquakes.

This study evaluates the ammonia (NH3) reduction effect of Bio-curtains (hereinafter referred to as curtains) utilized for odor control in pig farms based on the distance outside the curtain and the spraying condition. The curtain (total area: 37.9m3) was constructed with two layers of light-shielding screens stretched over a rectangular parallelepiped structure installed around a ventilation fan (630 mm) on the side wall of a pig barn where 48 finishing pigs were reared. The real-time NH3 concentration was measured by using a photoacoustic spectrometer. In the first part of this study, the NH3 measurement position for each side of the curtain was selected based on the lowest standard deviation among 4 to 8 initial sampling points on the surface of the curtain and utilized for both experiments of distance and spraying. In the experiment concerning the distance outside the curtain, ammonia concentration decreased by 17.45% at 2m compared to the distance at 5 cm and by 6.94% at 4m compared to 2m on average. In contrast, the NH3 reduction rate for each distance compared to the ventilation fan was the lowest at the 100% operating rate in which the exhausted NH3 concentration from the ventilation fan was low. At this time, the spraying mist on the inside of the curtain increased the NH3 reduction rate by 4.98 to 10.36% compared to the non-spraying condition. Consequently, the NH3 be reduced as distance outside the curtain increases due to the diffusion effect caused by the surrounding wind and the spraying mist on the inside curtain on the dissolution of NH3.

PURPOSES : Recently, increasing number of local governments are introducing on-demand public transportation service in real time to improve the management efficiency of public transportation. In preparation for the autonomous driving era, the “autonomous public transportation mobility on-demand service in real time” is being developed in the public transportation sector to introduce on-demand public transportation. For this service to become the public transportation of the future, it must receive publicity. Therefore, in this study, indicators were selected to evaluate the publicity of the autonomous public transportation mobility on-demand service in real time, and a survey was conducted among the general public to examine the validity of the indicators. METHODS : To secure the publicity of on-demand public transportation in real time, the goal of the service was first set. Second, the keywords of the service were analyzed to define the publicity requirements of this service. Based on the analyzed keywords and definition of publicity, service indicator types were classified, and the indicators were defined by type. A user satisfaction survey was conducted on the final selected indicators to improve the degree of completion of indicator development. The user survey was conducted by presenting selected publicity indicators to respondents responding on a five-point scale to determine whether each indicator could satisfactorily evaluate publicity. RESULTS : When examining various previous research cases and the contents required for autonomous public transportation mobility on-demand service in real time, the required items of publicity indicators were found to be “safety” in terms of “autonomous driving” and “convenience” in terms of “demand response.” Publicity indicators were developed according to these three items. Thirty-one indicators were developed, and a satisfaction survey was conducted on the general public for each indicator. In the survey, most of the indicators scored 3.5 points or higher, indicating that the indicators were generally well defined. Users gave the highest score to “fairness” among the three items, and “reasonable fairness” was found to be a necessary item as a publicity indicator. CONCLUSIONS : In this study, evaluation indicators were selected to secure the publicity of autonomous public transportation mobility on-demand service in real time, and a satisfaction survey was conducted for each indicator. Most of the indicators showed a score of 3.5 or higher, indicating that they were generally well defined. However, this study has a limitation in that it surveys the general population. In future, experts should be included in the survey to increase the degree of completeness of the public indicators.

HIC refers to a radwaste packaging container that can maintain integrity for more than 300 years in the general underground environment and disposal conditions in Korea. For HIC, the integrity of containers is verified according to the HIC regulation guideline for LLW and ILW disposal. Existing material tests include mechanical stability, permeability resistance, corrosion properties, chemical durability and biological resistance. In this study, a chemical durability test was conducted to prove the suitability of the HIC material by measuring the degree of chemical influence other than corrosion from the disposal environment. The chemical resistance evaluation method was used to simulate the disposal environment in the underground repository, and the amount of change in the physical properties of the degraded polymer concrete specimens according to the test time was confirmed. The technical standards considered leaching of material components, sulfation attack, acid attack, alkali, carbonate, and salt crystallization. The compressive strength and weight change of the specimens with time were checked by immersing them in a chemical solution that could leak major hazardous substances and wastes in the groundwater of the repository for several months. In addition, in order to evaluate the integrity in condition severe than the disposal environment, a flow was applied to a chemical solution having a concentration twice that of the basic chemical resistance test conditions, and the test period was extended twice to accelerate the deterioration of the specimen.

PURPOSES : The purpose of this study was to evaluate the newly developed Guss mastic asphalt mixtures, called EQ-mastic asphalt mixtures, which contain melted additives for decreasing cooking time. METHODS : A series of experiments were performed to investigate the effectiveness of the melted additives in EQ-mastic asphalt mixtures. Both the existing Guss mastic asphalt mixture and the EQ-mastic asphalt mixture were produced with the same amounts of asphalt binders, aggregates, and fillers, but the existing Guss mastic asphalt mixture contained 3% Trinidad lake asphalt (TLA). The EQ-mastic asphalt mixture contained 3% of additives, including TLA and polyolefin. The physical material performances of both mastic asphalt mixtures were obtained by conducting the Luer fluidity test, penetration test, dynamic stability test, and low-temperature bending test. The results of the tests for the existing Guss mastic and EQ-mastic asphalt mixtures were compared. RESULTS : The fluidity, penetration, dynamic stability, and low-temperature bending strains of both the existing Guss mastic and EQmastic asphalt mixtures all satisfied the standard values provided in the production and construction guides of the Guss mastic asphalt pavement. CONCLUSIONS : When melted additives containing polyolefin are used in the production of Guss mastic asphalt mixtures, the cooking time decreases, so that the corresponding energy consumption and asphalt fume amount can be reduced. Therefore, an EQ-mastic asphalt mixture is proposed for use as an eco-friendly pavement material.

본 연구에서는 동맥스핀라벨링을 이용하는 비 조영증강 기법인 4D TRANCE으로 동정맥기형 환자를 진단함에 있어 기존에 사용하고 있는 4D TRAK과 조영증강 3D TOF으로 얻는 진단적 정보의 재현성과 유용성을 알아보고자 하였다. 연구방법 은 동정맥기형 추적검사를 하는 환자 14명을 대상(남자 4명, 여자 10명)으로 하여 뇌혈관 영상을 각각 4D TRANCE, 4D TRAK, 조영증강 3D TOF를 순서로 획득하였다. 영상의학과 전문의 2명이 3 영상의 진단적 평가와 영양동맥(feeding artery, FA)과 배출정맥(draining vein, DV)을 비교 평가하였고, 동일한 부위의 병소(nidus)와 내경동맥에 대한 대조도 잡음비를 비교 분석하였다. 정성평가는 4D TRANCE는 진단적 정확도 3.93(3.89~4.04), FA 3.75(3.55~3.95), DV 1.96(1.77~2.16) 으로 모든 구간에서 4D TRAK과 비교하여 통계적으로 유의하였으나(p＜0.001), 유일하게 DV에서 유의하지 않았다. 정량 분석에서는 4D TRANCE와 3D TOF의 대조도 잡음비에 따른 독립표본 T검정 결과, 조영증강되는 병소에서는 4D TRANCE가 3D TOF에 비해 15.50의 차이로 높았으며, 통계적으로 유의하였다. 본 연구는 동정맥기형 추적검사에 대해 4D TRANCE는 4D TRAK보다 다소 저하된 영상품질을 보여주었으나, 3D TOF와 비교했을 때 혈관 해부학 및 혈류역학 정보를 제공하기 때문에 더 신뢰할 수 있는 정보를 제공하여 임상 영상 평가에 유용하게 사용할 수 있다.

PURPOSES: This paper presents the development and evaluation of the smart hardware-in-the-loop systems (SMART-HILS) that evaluate traffic signal operations of a new real-time traffic signal control system called SMART SIGNAL at the traffic management center (TMC) level. METHODS: The layouts of the hardware and software components of the SMART-HILS were introduced in this study and its performance was tested using real-time traffic signal operation algorithms embedded in the SMART SIGNAL control server by utilizing the VISSIM simulation model. In this study, the SMART-HILS management software was developed using .NET programming language. Fewer random seed numbers were used for the test scenarios by conducting statistical tests to address the shortcomings of a longer time due to the adoption of the simulation time as the real-time by the TMC server. RESULTS : It was determined that SMART-HILS can communicate with TMC and VISSIM for both upload and download directions within acceptable time constraints and evaluate new design algorithms for traffic signal timing. CONCLUSIONS : In practice, traffic engineers can utilize SMART-HILS for testing the traffic signal operation alternatives before their selection and implementation. This application could increase the productivity of traffic signal operation.

본 연구는 국내에서 육성된 중생종 동양배 ‘신화’의 과실 생장 및 성숙기간 중의 품질요인을 평가하고 수확시기별 1-methylcyclopropene (1-MCP) 훈증처리 효과가 25℃ 유통 중 과실품질 및 생리장해 발생에 미치는 영향을 구명하고자 실시하였다. 만개 후 125일부터 153일까지 1주일 간격으로 품질요인을 조사한 결과, 경도는 경시적으로 저하되어 만개 후 146일인 9월 6일에 29.9N이었고, 만개 후 153일에는 21.5N으로 급격히 경도가 저하되었다. 과실성숙의 진행과 더불어 알콜불용성물질 함량은 지속적으로 낮아짐과 동시에 가용성고형물함량이 증가하여 만개 후 146일에 각각 8.13mg·g FW-1 및 11.2oBrix 로 조사되었다. 성숙기간 중 과당이 우점하고 있었고, 과실성숙도가 높아질수록 자당의 비율이 유의하게 증가하는 경향이었는데 만개 후 153일에 수확한 과실에서도 자당이 과당을 넘어서 우점하지는 않았다. 수확 시기별 1.0μL·L-1 1-MCP 훈증 처리가 유통 중 무처리구 과실에 비해 높은 경도를 유지하였다. 수확시기 간에는 만개 후 146일에 수확한 과실에 대한 1-MCP 처리가 만개 후 153일 수확 과실에 비하여 유통 중 경도 유지 효과가 높았다. 바람들이 장해의 발생은 만개 후 146일에 수확한 과실은 유 통 28일 이후 증가하기 시작하였고, 만개 후 153일 수확 과실의 경우에는 유통 21일부터 급증하였는데 1-MCP 처리는 ‘신화’ 배의 생리장해 발생을 경감하지 못하였다.

본 연구는 2012년 1월부터 2019년 1월까지 과천경마공원에서 경주한 더러브렛, 6,762두의 주파기록, 총 82,796개를 이용하여 동기군의 구성을 조사하기 위하여 수행되었다. 모든 경주거리에서 우승마의 주 파기록이 1초 감소하면 해당 경주의 평균 주파기록은 0.89초에서 0.99초 감소하였다. 주파기록에 대한 동기군의 효과는 총변이의 약 41%를 차지하였으며, 참가경주의 효과는 일괄적으로 모든 경주거리에서 가장 크게 나타났다. 동기군을 포함한 모형(Ⅰ)과 우승마 기록을 포함한 모형(Ⅱ)에서 추정된 주파기록에 대한 유전분산은 각각 0.4760과 0.4148, 유전력과 반복력은 각각 0.26, 0.47과 0.22, 0.40이었다. 모형 Ⅱ에서 추정된 유전분산과 유전력은 모형 1에 비하여 각각 13%와 15% 감소하는 것으로 나타났으며, 우승마의 주파기록을 환경효과로 모형에 포함하는 것보다는 동기군을 포함하는 것이 주파기록을 량하는데 있어서 더 유리할 것으로 판단되었다.

Angiotensin receptor blockers, such as telmisartan, are considered effective in the treatment of hypertension and proteinuria due to chronic kidney disease (CKD) in cats. It selectively blocks the AT1 receptor and does not affect the AT2 receptor, thus effectively blocking the activity of the renin angiotensin aldosterone system. This study aims to compare over time the changes in various indicators, including systemic hypertension and proteinuria, before and after the administration of telmisartan in cats with CKD. Decrease in blood pressure (BP) (p<0.001) and urine protein to creatinine (UP/C) ratio (p<0.001) were found to be statistically significant over time after the administration of telmisartan. BP and the UP/C ratio were 160 ± 22.2 and 0.50 ± 0.647 before telmisartan administration (Day 0), 150 ± 21.0 and 0.27 ± 0.487 on the 30th day (Day 30), 150 ± 17.0 and 0.25 ± 0.376 on the 60th day (Day 60), and 140 ± 17.8 and 0.15 ± 0.233 on the 90th day (Day 90) after administration, respectively. BP and UP/C were statistically significantly lower in cats with CKD over time at each time point from Day 0 to Day 90 at 30 day intervals. Especially after 90 days of telmisartan administration, the improvement of BP and UP/C were estimated to be about 20 mmHg and 0.35, respectively. In conclusion, the oral administration of telmisartan to cats with CKD is effective in improving BP and proteinuria, which has a positive effect on long-term survival in cats with CKD.