본 연구에서는 연안으로 유출 위험이 있는 에마멕틴 벤조에이트(emamectin benzoate, EB) 성분의 농약에 대한 넙치의 폐사, 혈액학적 변화, 조직학적 변화를 확인하였다. EB 성분 농약을 96-well plate에서 48시간 동안 0.215, 2.15, 21.5, 215ppm의 농도로 EPC 세포에 노출한 후 세포 독성을 확인한 결과, 21.5ppm과 215ppm의 농도에서 세포 박리가 확인되었고 215ppm에서는 4.12±1.8%의 세포 생존율이 확인되었다. 넙치를 polypropylene수조(용량 300L)에 순치하고, EB 성분 농약을 침지법으로 48시간 동안 노출한 결과, 2.15ppm 21.5ppm 농도에서는 넙치가 모두 폐사했지만, 0.0215ppm과 0.215ppm 농도에서는 폐사가 관찰되지 않았다. 혈액학적 분석 결과, 혈장 glucose 농도는 21.5ppm 노출 넙치에서 control 대비 1.52배 증가하였고, total protein 농도는 2.15ppm, 21.5ppm 농도에서 control 대비 각각 0.71배, 0.74배 증가하였다. Cholesterol, alkaline phosphatase은 21.5ppm 농도에서 control 대비 각각 2.84배, 2.44배, 3.8배 증가하는 것을 확인하였다. Aspartate aminotransferase와 alanine aminotransferase는 21.5ppm 농도에서 control 대비 각각 0.4배 및 0.5배 증가하는 것을 확인하였다. 이상의 결과로, 21.5ppm 이상의 농도는 넙치의 급성 폐사를 유발하며, gluscos수치 증가로 인해 스트레스 증가 및 간과 신장 손상에 의한 혈액학적 변화를 유발하는 것을 확인할 수 있었다. 한편 EB 성분 농약을 48시간 동안 단기간 처리하는 것에 의해서는 간 조직에 병변을 유발하지는 않았다.
본 연구는 8종의 생약 복합추출물인 Derma Genie™-DLP121의 피부 건강 개선 효능을 확인하 고자 하였다. 이를 위해, in vitro model에서 Derma Genie™의 생리 활성 정도를 평가했으며, 4종의 피부 세포 내 항산화, 항염, 보습 증진, 주름 개선 및 미백 효능을 분석하였다. 실험 결과, Derma Genie™에는 22.577 mg GAE/g의 폴리페놀이 함유되었고, DPPH assay에서는 최대 74.947%의 radical 소거능을 보였 다. Lipopolysaccharide를 처리하여 과염증 반응을 유도한 RAW 264.7 세포에서는 Derma Genie™가 산 화 질소(NO) 뿐만 아니라, 3종의 염증성 사이토카인(Il-6, Il-1β, Tnf-α)의 농도 의존적인 감소 효과를 매개하여 상당한 항염 효능이 있음을 입증하였다. 또한 Derma Genie™는 히알루론산을 생성하는 효소인 HAS1 및 HAS2의 유전자 발현은 증가시키고, 히알루론산 분해 효소 HYAL1의 발현은 저해시켜 세포 내 보습 증진 효능을 나타내었다. 뿐만 아니라, ultraviolet B (UVB)에 의한 세포 생존율 감소 및 주름 연관 유전자(COL1A1, COL3A1)의 발현 감소가 Derma Genie™ 처리 시 개선됨을 확인하였다. 특히 최대 농도 의 Derma Genie™ (1 mg/mL)와 UVB를 병용 처리한 군에서 주름 연관 인자인 COL1A1 및 COL3A1의 발현량이 각각 55.080 및 57.103% 개선됨을 보였다. 마지막으로, α-MSH로 멜라닌 합성을 유도시킨 B16F10에서 Derma Genie™의 병용 처리는 세포 내 멜라닌 함량을 최대 95.159% 감소시켰다. 이는 Derma Genie™가 세포 내 tyrosinase 활성을 억제함으로써 미백 효능을 가지는 것으로 판단되었다. 따라 서 이러한 결과는 Derma Genie™가 피부세포 내 항산화, 항염, 보습 증진, 주름 개선 및 미백 효능이 존재 함을 보여주며, 피부건강 증진을 위한 신규 기능성 화장품 원료로의 활용 가능성을 제고하였다.
Food waste is a critical problem for many countries. Food producers and groceries often discard imperfect foods or food by-products that still contain nutritional value. To address this problem, some food manufacturers have turned to upcycling, that is, to convert otherwise discarded ingredients into new food products (e.g., cacao fruit pulp into crunch bites). Consumers’ acceptance of sustainable products is generally lower than that of conventional products due to quality concerns. We speculate that for upcycled food products, consumers’ perception of product quality may vary when different percentages of imperfect ingredients are integrated into the products. Drawing from schema congruity theory, this research examines how the usage of imperfect ingredients can impact the perceived quality of upcycled food products. The implications for marketing upcycled foods are discussed.
Inaccurate amounts of active ingredients in disinfectants can result in inefficiency or in potential toxicity to the environment and living organisms. This may lead to disinfection failure, and consequently, biosecurity failure. To ensure the efficiency of disinfectants and their ongoing compliance with safety and quality requirements, continuous and systematic post-market surveillance studies are needed. Herein, the content of ten commercial disinfectants purchased in 2021 was analyzed. Selective analytical techniques, such as automated titration, colorimetry, and high-performance liquid chromatography, were used to evaluate the content of several active ingredients present in disinfectants, such as potassium peroxymonosulfate, benzalkonium chloride, glutaraldehyde, phosphoric acid, citric acid, and malic acid. The obtained values were then compared with the label claims; the active ingredient contents of all disinfectants were within the acceptable range of 90–120% of the label claim.
This study aimed to evaluate the changes in the content of functional components and antioxidant activity of rice and barley according to the milling process used. A considerable amount of γ-oryzanol was observed in unmilled rice and barley. However, γ-oryzanol was not detected in the rice and barley after the milling process. The highest content of γ-aminobutyric acid (GABA) was observed in the unmilled Keunalbori-1-ho barley. The contents of biotin in all samples also decreased in the milled grains compared to the unmilled grains. The highest content of total polyphenols and flavonoids was observed in the Heuksujeongchal barley, and the highest radical scavenging activity was also found in this grain. The milling process led to a decrease in the content of functional components, including γ-oryzanol, GABA, biotin, polyphenols, and flavonoids in both rice and barley. These results may be useful in the development of processed foods using cereal grains.
이 연구는 열역학적으로 불안정한 활성성분을 안정하게 봉입하기위해 초임계 상태에서 다중층 의 리포좀을 생성하고 유효성분을 봉입하는 것에 관한 것이다. 초임계 상태에서 원활하게 리포좀을 형성시 키기 위하여 식물성유래의 하이드로제네이티드 포스파티딜콜린과 그 유도체, 하이드로제네이티드 수크로 오스다이스테아레이트를 포함하는 혼합 계면활성제를 고순도로 합성하였다. 이것을 반응조에 이산화탄소 를 주입하여 초임계상태를 만들고 교반함으로써 거대 리포좀을 생성시키고, 여기에 제니스테인(genistein) 과 쿼세틴(quercetin)을 첨가하여 봉입하는 제조방법에 대하여 기술하였다. 혼합지질계면활성제(SC-Lipid Complex)의 HLB는 12.50이었으며, 아주 낮은 농도에서도 다중층의 리포좀 소포체가 형성되었다. 이 계면 활성제의 외관은 엷은 황색의 페이스트로 특이취가 있었으며, 비중은 0.972이었고, 산가는 0.12로 고순도 로 합성이 되었음을 알 수 있었다. SC-Lipid Complex를 사용하여 20 wt%의 카플릭/카프릭 트리글리세라 이드와 트리에칠헥사노인을 사용한 유화력 실험결과 96.2 %의 유화력을 가지고 있음을 알 수 있었다. 제 니스테인을 봉입한 초임계 리포좀에 대하여 투과전자현미경(Cryo-TEM)을 통해 다중층의 리포좀 소포체 가 형성되었다는 것을 확인하였다. 제니스테인이 봉입된 1차 리포좀화한 입자 크기는 253.9 nm이었고, 2 차 캡슐의 크기는 18.2 ㎛ 이었다. 제니스테인을 표준물질로 하여 초임계 상태 리포좀의 봉입효율은 99.5 %이었고, 일반적인 리포좀은 93.6 %의 효율을 가지는 것으로 나타났다. 또한 쿼세틴을 봉입한 항산화력 실험은 DPPH법으로 확인한 결과 초임계리포좀에서 유의성 있게 우수한 항산화력을 유지하고 있음을 알 수 있었다. 이러한 결과를 바탕으로 효과는 우수하나 열역학적으로 불안정한 원료를 유기용매를 사용하지 않고 초임계 상태에서 리포좀에 봉입하고, 고기능성의 스킨케어 화장품, 메이크업 화장품, 두피보호용 화장 품 등 다양한 제형에 응용이 가능할 것으로 기대한다.
The main purpose of this study was to examine the correlation between the consumption of red ginseng-based ‘SSR’ for 30 days and the reduction in human fatigue, blood component changes, and immune cell activity in 35 human subjects. ‘SSR’ is composed of zinc oxide, folic acid, and D-α-tocopherol with red ginseng as the main component. According to the protocol criteria of the study, 35 subjects who understood the purpose of the study and signed an informed consent form were selected. The fatigue survey was conducted through a questionnaire, and after taking ‘SSR', a decreased tendency of physical, mental, and neurosensory fatigue was observed. In hematological analysis, no significant changes were observed in the levels of WBC, RBC, and hemoglobin; however, AST (SGOT) and ALT (SGPT) levels were statistically significantly decreased. In immunological analysis, it was observed that the proliferative effect of T cells (CD3+CD4+) was greater than that of NK cells (CD16+CD56+). The collected data were subjected to t-test analysis using the SPSS 25.0 statistical program. The result from this study proposes that ‘SSR’ can be used as a functional food material as it reduces human fatigue and enhances immune function.
하수오(Polygonum multiflorum Thunb)는 다년생 덩굴성 식물로, 지하부인 뿌리(tuber)는 한의학에서 약재로 다양한 질병의 치료에 사용되고 있다. 하수오를 한약재로 사용하기 위하여 약용물질의 증진 또는 식물의 독성을 줄이기 위해 1차 가공 및 포제를 한다. 본 연구에서는 하수오 포제에 있어 다양한 보료처리를 통해 주요물질의 유효성분 함량을 확인 해 보고, 하수오 포제에 적합한 보료를 검증하는 실험을 진행하였다. 또한, 실험에 두 가지 타입의 하수오를 활용하여 기존 유통품 하수오와 조직배양 연구를 통해 개발된 KIOM 특허 기반 하수오를 비교하는 실험을 진행하였다. 하수오의 주요 지표물질인 emodin의 함량이 참깨추출물을 보료로 사용한 처리구를 제외한 나머지 보료 처리구(흑두즙, 쌀뜨물, 감두즙, 로즈마리추출물)에서 KIOM품이 유통품보다 높게 측정되는 것을 확인하였다. 특히 KIOM품은 기존에 알려진 하수오 보료인 흑두즙보다 감두즙을 보료로 사용해 포제 하였을 때 0.29 ug/mg 더 높은 emodin의 함량이 확인 되었다. 본 연구결과를 통해 하수오의 약용자원으로 사용함에 있어 앞으로 KIOM하수오의 생산과 포제에 관련된 연구가 더 이루어 져야 할 것이며, 연구 결과는 하수오의 포제에 있어 표준화와 현대화를 위한 기초 지식 및 결과로 사용 될 것이라고 사료된다.
Barley is an important cereal gain which is traditionally used in some nations of Asia and North Africa, and there has been growing interest in using barley as an ingredient in food due to their nutritional value and high content of phyto-constituents. However, no study report on comparative feed value between sprouted barley, cornflake and alfalfa hay. Therefore, in this study we aimed to evaluate the chemical composition, amino acid profile and mineral content of 6 day sprouted barley fodder (SBF) compared with cornflake and alfalfa hay using by AOAC method, as an alternative feed ingredient. Results showed that SBF had higher content of crude protein, acid detergent insoluble crude proteins and neutral detergent insoluble crude protein than alfalfa hay and cornflake; cornflake had higher crude fiber, neutral detergent fiber content than SBF and alfalfa hay; alfalfa hay had higher crude fiber, crude ash, acid detergent fiber, neutral detergent fiber and lignin level than SBF and cornflake. Also, significant differences were found on amino acid content among them (p<0.01). The most abundant amino acid in SBF was glutamate (123 g/kg DM), which is higher than in alfalfa hay (1.27%) or cornflake (1.58%). However, methionine (1.33%) and cysteine (1.53%) were the least abundant amino acids in SBF compared with cornflake or alfalfa hay. Furthermore, our study results exhibited that SBE comprise a good sources of minerals including ferrous (90.01 mg/kg) followed by zinc (20.50 mg/kg), magnesium (0.20 mg/kg) and sodium (0.03 mg/kg) as compared to cornflake and alfalfa hay. The present research findings, confirmed that the nutritional values of SBF are comparable to those of cornflake and alfalfa hay. Hence, SBF can be a better alternative feed ingredient for cornflake or alfalfa hay. However, feeding trials will be required to determine acceptability of SBF for ruminant production.
This study aims to explore the motivations and effects of country of ingredient authenticity on product judgement and willingness to buy or/and recommend. Country of ingredient authenticity can be defined as set of consumers’ generic characteristics or traits of seeking/pursuing/consuming raw materials/goods and/or artisan skills that are deemed to be superior and synonymous to a specific location in the pursuits of one’s self image and social standing (Cheah, Zainol and Phau, 2017). The jewellery brands used in this study are Swarovski, Pandora and Monora. The 4X1 exploratory research design looks at four different conditions applied to the three jewellery brands. AMOS and SPSS were used to analyse the data and determine model fit. Managerial implications include the positive recognition of the ingredient authenticity of Swarovski crystals incorporated with other products/brands.
Theoretical Development
Country-of-origin acts as a quality indicator (Bilkey & Nes, 1982). Consumers rely on their perceptions of product quality from a specific country to evaluate other products from the same country. The home country of a brand and its image can serve as a source of competitive advantage or disadvantage (Cuervo-Cazurra & Un, 2015). In more recent times, product collaborations and ingredient branding techniques have been adopted in the marketplace. As a first for its brand, Louis Vuitton partnered with Supreme to launch a streetwear line in 2017. In 2011, Prada launched a ‘made in’ campaign to highlight the authenticity of the raw materials and artisan skills used to create the products. Country-of-ingredient-authenticity aimed at exploring how countries can capitalize on their expertise to improve product and country image perceptions. The incorporation of materials and skills from the country-of-ingredientauthenticity can be a strategic decision that allows brands to alleviate the product judgement and willingness to buy or recommend (Suter et al., 2017). This decision can prove to be advantageous for the firm (Suter et al., 2018). This study aims to uncover whether Swarovski crystals, as both a raw material and artisan skill, can affect consumers’ perception of the product judgement and willingness to buy and recommend. The study also profiled the motivations of consumers’ seeking these ingredient-authentic products. In this research, statusseeking consumption and fashion knowledge act as the underpinning background variable to the model (Flynn et al, 2000; Eastman et al 1999). Luxury consumption is driven by the need for status in conspicuous luxury consumers whereas fashion knowledge in inconspicuous luxury consumers (Jaikumar, Singh and Sarin, 2018; Koo and Im, 2017). The study aims to discover whether status-seeking consumption and fashion knowledge has a significant impact on consumers’ need for seeking authentic ingredients in jewellery.
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Research Design
In order to test our hypotheses, an online Qualtrics survey was created and administered to an Indonesian sample aged between 18 to 55 years old. Four sets of surveys were created to reflect the 4 different stimulus (Table 1). The study aims to look at whether the different brands paired with different ingredient authentic materials would result in a different perception of product judgement and willingness to buy/recommend (Maxham and Netemeyer, 2003; Bower and Landreth, 2001; Wood and Darling, 1993). The researchers were also interested to uncover the motivations behind the need for ingredient authenticity in consumers’ decision making to buy jewellery. The brands chosen were Swarovski, Pandora and Monora. Swarovski serves as the control to measure consumers’ response to the actual brand with the incorporated Swarovski crystals. Pandora was used as it is a well-known jewellery brand with a potential collaboration with Swarovski and Monora is a lesserknown jewellery brand whose brand and product quality could improve with the incorporation of Swarovski crystals. Respondents chosen had to be interested in or aware of luxury branded goods as it is important to possess some knowledge to seek for authenticity in luxury branded products. 600 completed surveys were collected in a span of 2 months. AMOS 21 software was used to run SEM model and test for model fit. SPSS 22 was used to run a median split and independent sample T-test to ascertain the motivations of ingredient-authenticity behaviour between low and high status seeking and fashion knowledge consumers.
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Results
Four models were tested on AMOS to determine the model fit and its significant interactions.
Study One – Swarovski jewellery with Swarovski crystals
The conceptual model has achieved a good model fit with indices of χ2(94) = 128.937, p = .01. The other indices suggest adequate fit, SRMR = .0303, GFI = .910, IFI = .988, TLI = .985, CFI = .988, RMSEA = .049, PCLOSE= .510.
Study Two – Pandora jewellery with Swarovski crystals
The conceptual model has achieved a good model fit with indices, of χ2(34) = 63.490, p = .002. The other indices suggest adequate fit, SRMR = .0415, GFI = .933, IFI = .980, TLI = .968, CFI = .980, RMSEA = .076, PCLOSE= .071.
Study Three – Pandora jewellery with real crystals
The conceptual model has achieved a good model fit with indices, of χ2(54) = 56.332, p = .388. The other indices suggest adequate fit, SRMR = .0233, GFI = .945, IFI = .999, TLI = .998, CFI = .999, RMSEA = .017, PCLOSE= .900.
Study Four – Monora jewellery with Swarovski crystals
The conceptual model has achieved a good model fit with indices, of χ2(109) = 182.549, p = .000. The other indices suggest adequate fit, SRMR = .0464, GFI = .893, IFI = .975, TLI = .968, CFI = .974, RMSEA = .066, PCLOSE= .06.
A median split and independent sample T-test were carried out on low and high status-seeking and fashion knowledge consumers and their means compared to the ingredient-authentic seeking behaviours. Status-seeking behaviour is the main driver of consumers acting on the need for authenticity of raw materials and artisan skills on all 4 stimulus. This behaviour could be attributed by consumers’ hedonic need to own jewellery that signal good quality and design. Fashion knowledge consumers seek authenticity of raw materials and artisan skills in both Pandora stimulus and looked for authenticity of artisan skills in Monora stimulus. This indicates that owning a Pandora jewellery can be attributed to being a trendy fashionista following the current trend of collecting charms. As for the lesser known brand, Monora, the incorporation of the ingredient authentic Swarovski crystal helped consumers to identify the authentic artisan skill that goes into making the jewellery.
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Conclusion
Swarovski jewellery commands high interest in consumers seeking for ingredient authentic products as authenticity of artisan skills positively influences product judgement and authenticity of raw materials positively influences willingness to recommend. There was no ingredient-authenticity effect in consumers when exposed to Pandora jewellery with Swarovski crystals. Pandora has always been associated and known for the novelty of collecting the charm trinkets. Consumers are more likely to buy Pandora for their charms rather than the fact it is embedded with a Swarovski crystal. Pandora jewellery with real crystals was perceived positively by consumers to increase product judgement and willingness to recommend and buy. Again, the collectors’ mindset plays a role in this behaviour. The strong brand effect also plays well into Pandora’s popularity in the marketplace. Monora jewellery with Swarovski crystals piqued consumers’ interest in the ingredient authentic product with authenticity of artisan skills positively influencing product judgment and authenticity of raw materials positively influencing willingness to recommend. The results support the idea that when Swarovski crystal is incorporated into a lesser known brand’s product, it increases the quality perception and word of mouth value. On all four studies, consumers’ product judgment positively influences willingness to recommend indicating consumers are more willing to spread word-of-mouth when they believe the product is of good quality. This willingness to recommend then positively leads to willingness to buy the product. Status-seeking behaviour was seen as a main factor for consumers when searching for the ingredient-authenticity of the jewellery. For Swarovski and Monora, the ingredient played a part in inducing the ingredient-authenticity behaviour whereas owning a Pandora jewellery signifies some sort of in-class group reference and trend. The Pandora results are supported when consumers who have fashion knowledge also sought the ingredient-authenticity of the Pandora product.
Ingredients are the component parts or elements of something. Brands often portray a product’s ingredients, components, and parts as humanlike messengers in advertising and packaging. Nature’s Way, for example, humanizes a strawberry for their Vita Gummies by portraying it with eyes and a mouth. This study explores whether imbuing ingredients or components with humanlike features influences consumer perceptions and decisions. The results of Study 1 demonstrated that anthropomorphizing an ingredient successfully intensified consumer purchase decision in a coffee shop context. However, not all humanized ingredients were effective. The data from Study 2 revealed that anthropomorphizing a primary ingredient was more likely to increase purchase intentions than anthropomorphizing a secondary ingredient. This effect was mediated by quality perceptions. While prior research investigated the effect of product anthropomorphization on consumer responses, our study mainly focused on humanizing the ingredients that constitute a product, an important topic that has not yet been investigated in the marketing and consumer research.
This study attempted to establish an ultra performance liquid chromatography (UPLC) analysis method for standard determination of silymarin as a health functional food material in Silybum marianum extraxt (milk thistle). UPLC was performed on a Waters Acquity BEH C18 (50×2.1 mm, 1.7 μm) column using a gradient elution of distilled water and methanol at a flow rate of 0.21 mL/min and detection wavelength of 288 nm. The UPLC method showed high linearity in the calibration curve at a coefficient of determination (r2) of >0.9999, and limit of detection and quantitation for 6 flavonolignans were 0.0167-0.2469 and 0.1648-1.2931 μg/mL, respectively. The recovery of each flavnolignan was in the range of 99.96-100.81%, and the relative standard deviation for precision of each flavonolignan was less than 1.0%. The UPLC method established in this study was more specific for the quantitative determination of silymarin than the HPLC method. Also, since the UPLC method is shorter in the equipment operation time and smaller in the amount of used solvent than the HPLC method, UPLC is expected to have higher energy efficiency and lower environmental impact compared with HPLC.
The purpose of this study is to investigate the effects of oxides (caryophyllene oxide, eucalyptol and linalool oxide), one of the major ingredients of essential oils, on odor intensity, electroencephalography (EEG) response and emotional response. The subjects were women in their twenties. Inhalation concentrations of oxides were varied from 0.01 to 10%, and EEGs were collected by Quantitative EEG. The odor intensity and average preference index (API) of the oxides were ranked in the order of eucalyptol > linalool oxide > caryophyllene oxide. Eucalyptol is a refreshing and pleasant substance that activates the brain (activation of β and γ waves and spectral edge frequencies (SEF50 and SEF90)). Linalool oxide has a calm and soft substance (activation of θ, inactivation of α, β, and γ waves) at a low concentration (<0.1%), and a fresh smell at high concentration (inactivation β and activation of θ and γ). Caryophyllene oxides were evaluated as a substance that is comfortable, soft, calm and sedating, which deactivates the brain (activation of α, inactivation of β and γ). Oxide materials showed a positive correlation between the odor intensity and the SEFs. Each of the oxides showed unique characteristics in the areas of odor intensity, emotional response, API, and EEG. These results give us a better understanding of the properties of the pure materials that make up a fragrance, and provide useful information for the manufacture of fragrance products or aroma oil blends with enhanced specific functions.