This study evaluated the microbiological quality of fresh-cut bell pepper and ginger, as well as the inactivation effects of intense pulsed light (IPL) on E. coli ATCC 25922 inoculated in the fresh-cut samples by varying the treatment voltages (1,200-2,400 V) and time (1–7 min). The contamination levels of mesophilic bacteria, psychrophilic bacteria, yeast and mold for bell pepper and ginger were 6.64±0.81 and 6.35±1.96 log CFU/g, 6.75±1.13 and 5.63±1.89 log CFU/g, and 4.68±0.43 and 4.57±1.25 log CFU/g respectively. Through the IPL treatment at 2,400 V for 7 min, 2.04 and 2.11 log of E. coli ATCC 25922 inoculated in bell pepper and ginger were reduced, respectively, with a negligible temperature rise (< 2.2oC). Although the reduction rate varied, the bactericidal effect of E. coli ATCC 25922 showed an increase as treatment time and voltage increased. Under the same treatment conditions, temperature increased by 1.71oC and 2.13oC for bell pepper and ginger, respectively. These results demonstrate that IPL is a suitable device for inactivating E. coli ATCC 25922 on fresh-cut bell pepper and ginger.
Because of many benefits from raw seed sprouts, consumers have consumed them largely. However, despite of many benefits, raw sprouts has been implicated in food-borne diseases. The source of food-borne disease related to raw seed sprouts is thought to originate from seeds contaminated by pathogenic bacteria. So, Intense Pulsed Light (IPL), a non-thermal processing method, is an effective device for seeds to maintain microbial safety without loss of seed viability. The objective of this research was to determine the effects on microbial inactivation and quality in radish and wheat seed by IPL treatment and to figure out the correlation between inactivation of seeds and surface roughness value (Ra). At 5th day of germination, the average germination rate and shoot length of radish sprouts by IPL at total fluences of 121 J/cm2 were 95% and 5.8 cm. It was not significant compared to the control group. And log reductions of radish and wheat seeds by IPL showed 1.0 and 1.2, respectively. The results showed radish seeds have higher tolerance to IPL treatment than wheat seed. Also, radish seed had the rougher surface (Ra=2.85 μm) than wheat seed (Ra=0.55 μm). Therefore, IPL can decontaminate microbial population on seeds, but the effectiveness of IPL is dependent on the surface morphology of seeds.
Intense pulsed light(IPL) has been highlighted as an innovative nonthermal sterilization technology that can kill spoilage or pathogenic microorganisms by using short-duration pulses of intense broad-spectrum electromagnetic radiation. This paper examines the inactivation effects of IPL on Listeria monocytogenes, Escherichia coli O157:H7, and Pseudomonas aeruginosa inoculated on seafood products such as salmon, flatfish, and shrimps and evaluates the possibility of extending the shelf-life of seafood products. The results indicate that the inactivation of microorganisms increased with an increase in IPL energy density(J/㎠) and a decrease in the distance between the sample surface and the lamp. In addition, temperature increases on the fish fillets during the treatments were well controlled within the range of 5.7~9.8℃. The IPL treatment had a significant positive effect on the storage stability of seafood products at the storage temperature of 4℃ for 12 days. These results suggest that the storage period for fish fillets can be extended from 4 days to 6~8 days through the IPL treatment.