This study was conducted to research the effect of Cryptomeria japonica sawdust and branch on the Production of Fruiting body of Pleurotus eryngii. Quantity of fruit body was lower in 5.2things/ bottle but yield of fruit body was higher in 3.5things/ bottle about big fruit body than Douglas-fir sawdust medium on C. japonica sawdust medium, so yield of fruit body was more 45g than Duglas-fir sawdust medium. But in the C. japonica branch sawdust medium, both quantity of fruit body and yield of fruit body was remarkably lower 13.3things/bottle, 42.8g/bottle respectively than Duglas-fir sawdust medium.

도로주변에 식재되어 있는 프라타너스(버즘나무)는 매년 늦겨울이나 이른 봄에 가지를 전정하는데, 이것은 대부분 땔감 등 활용가치가 적어 본 연구는 프라타너스의 전정가지를 톱밥으로 제조하여 느타리버섯의 새로운 배지재료로 활용가능성을 검토한 결과 배지재료별 균사생장량은 프라타너스톱밥+쌀겨(9 : 1)에서 30일간 배양할 때 12.8cm로 가장 많은 생장을 보였고, 프라타너스톱밥+쌀겨(6 : 4)를 제외한 기타배지들의 균사밀도는 높게 나타났다. 생육 전·후의 배지재료별 무기성분중에서 T-N, P2O5, K2O함량은 모든 배지에서 생육 후 감소 하는 경향이었다. 자실체의 무기성분은 프라타너스톱밥+쌀겨(9 : 1)에서 T-N, K2O, CaO, MgO함량은 다른 배지 보다 높았으나, P2O5함량은 미송톱밥+쌀겨(8:2)에서 0.92%로 가장 많은 함량을 보였다. 그리고 균사배양일은 배지재료에 따라 26∼37일로 큰 차이를 보였고, 프라타너스톱밥+쌀겨(8:2)는 대조구인 미송톱밥+쌀겨(8:2)보다 유효경수, 대직경 및 대길이가 길어 병당(850cc) 88.6g으로 18%증수하였다.

종균의 종류에 따라 중온성 품종의 생산성이 어떻게 달라지는지를 확인하고자 연구를 수행하였다. 버섯의 발생 경향은 세 처리구 모두 비슷했으며, 성형종균과 액체종균 처리구는 2차발생까지 총수확량의 50% 이상, 4차까지 80%이상 발생되었다. 기형버섯조사에서는, 세 처리구 모두 2차 발생까지만 기형버섯이 발생되었다. 하지만 기형버섯 발생률에 있어서는 다른 처리구들에 비해 액체종균 접종구에서의 발생률이 낮았다. 수확량조사에서는 액체종균을 접종했을 때 배지당 411 g으로 처리구들 중 가장 많았고 10 g이 넘는 버섯도 309 g으로 톱밥종균과 성형종균에 비해 많았다. 그리고 발생된 자실체 개수는 톱밥종균과 성형종균 간에 유의성이 있었지만 자실체 개당 무게는 세 처리구 간에 유의성이 없었다.

These experiments were conducted to search for the optimum composition of media material and media size for vinyl bag culture using sawdust. Considering the incubation period and the successful culturing rate, the optimum media size was length 25cm diameter 14cm (weight 2.5kg). The saturation moisture amount of Oak sawdust was 251㎖/100g. It is a half of pine sawdust 495㎖/100g. In 10 treatments of media construction including Oak sawdust 100%, the optimum material mixing rate was Oak sawdust 80%+wheat bran 20% and Oak sawdust 80%+ bran 20% considering the hypha growing speed and hypha density. The optimum moisture amount in media for growing Lentinus edodes was 55%. Injection pipe with 20 holes in media was reduced the incubation periods to 11 days when 20cm length media and 32 days when 30cm length media. The best method for browning media surface was above 10℃ of hematocryal and punching the vinyl bag. This treat could shortened the browning periods to 54 days.

In this study we attempted to analyze the change of protein and mycelial activity during low temperature storage progressed. At first, esterase and peroxidase isozymes were detected at third and fourth subculture in Pleurotus ostreatus and peroxidase isozymes in Pleurotus eryngii were detected at first and second subculture but esterase isozymes showed almost same band patterns subculture advanced. The decolorization ratio of media color including indicator were decreased according to subculture advanced in P. ostreatus and P. eryngii. However there were not significantly difference of mycelial growth, fruit body yield, esterase isozyme band pattern and laccase activity of P. eryngii according to storage periods at low temperature. Therefore, it was possible to store sawdust spawn of P. eryngii at 4℃ for 120days.

This study was carried out to obtain basic data on physiological characteristics for an artificial cultivation of Grifola frondosa. Ten strains of G. frondosa were collected from Korea, China and Japan and those were isolated and tested. PDA medium was selected for the favorable culture medium. The optimal conditions for the mycelial growth of G. frondosa were 25℃ and pH 4∼5 respectively. The carbon and nitrogen sources for the optimum mycelial growth were fructose and peptone, respectively. Highest mycelial growth was observed when the C/N ratio was 10∼20. Significant differences among lines were found for production yield and crop cycle time. KME44009 had a short spawn run time, high yield, good color and quality compare with other strains. To find out the suitable medium composition of G. frondosa, physicochemical conditions were investigated. The T2 formulation (55:25:8:12 mixing ratio of oak sawdust, oak chip, wheat bran and soybean cake) showed the shortest time to complete the crop cycle and the highest yields(weight of fresh mushrooms harvested at maturity). Among the physicochemical factors, pH, crude oil and total nitrogen may affect mushroom yield. Therefore, development of suitable medium would benefit from increased production efficiency of G. frondosa mushrooms and be commercial potential.

These experiments were conducted to search for the optimum composition of media material and media size for vinyl bag culture using sawdust. Considering the incubation period and the successful culturing rate, the optimum media size was length 25cm× diameter 14cm (weight 2.5kg). The saturation moisture amount of Oak sawdust was 251㎖/100g. It is a half of pine sawdust 495㎖/100g. In 10 treatments of media construction including Oak sawdust 100%, the optimum material mixing rate was Oak sawdust 80%+wheat bran 20% and Oak sawdust 80%+ bran 20% considering the hypha growing speed and hypha density. The optimum moisture amount in media for growing Lentinus edodes was 55%. Injection pipe with 20 holes in media was reduced the incubation periods to 11 days when 20cm length media and 32 days when 30cm length media. The best method for browning media surface was above 10℃ of hematocryal and punching the vinyl bag. This treat could shortened the browning periods to 54 days.

In this study we attempted to analyze the change of protein and mycelial activity during low temperature storage progressed. At first, esterase and peroxidase isozymes were detected at third and fourth subculture in Pleurotus ostreatus and peroxidase isozymes in Pleurotus eryngii were detected at first and second subculture but esterase isozymes showed almost same band patterns subculture advanced. The decolorization ratio of media color including indicator were decreased according to subculture advanced in P. ostreatus and P. eryngii. However there were not significantly difference of mycelial growth, fruit body yield, esterase isozyme band pattern and laccase activity of P. eryngii according to storage periods at low temperature. Therefore, it was possible to store sawdust spawn of P. eryngii at 4℃ for 120days.

This study was carried out to obtain basic data on physiological characteristics for an artificial cultivation of Grifola frondosa. Ten strains of G. frondosa were collected from Korea, China and Japan and those were isolated and tested. PDA medium was selected for the favorable culture medium. The optimal conditions for the mycelial growth of G. frondosa were 25℃ and pH 4～5 respectively. The carbon and nitrogen sources for the optimum mycelial growth were fructose and peptone, respectively. Highest mycelial growth was observed when the C/N ratio was 10～20. Significant differences among lines were found for production yield and crop cycle time. KME44009 had a short spawn run time, high yield, good color and quality compare with other strains. To find out the suitable medium composition of G. frondosa, physicochemical conditions were investigated. The T2 formulation(55:25:8:12 mixing ratio of oak sawdust, oak chip, wheat bran and soybean cake) showed the shortest time to complete the crop cycle and the highest yields(weight of fresh mushrooms harvested at maturity). Among the physicochemical factors, pH, crude oil and total nitrogen may affect mushroom yield. Therefore, development of suitable medium would benefit from increased production efficiency of G. frondosa mushrooms and be commercial potential.

본 연구는 가축분뇨의 종류와 시용수준에 따른 사일리지용 옥수수의 생산성과 N과 P의 용탈량을 조사하여 친환경 조사료 생산을 위한 기초자료를 제공하기 위해 수행되었다. 본 시험은 건국대학교 초지 시험포장 내의 사양토로 충전된 Lysimeter(직경 30cm, 깊이 1m)를 이용하여 화학비료, 우분액비 및 톱밥발효돈분에 대하여, N 시용수준을 100kg/ha, 200kg/ha, 400kg/ha씩으로 시험구를 배치 수행하였다. 옥수수 건물 및 질소 생산성은

The yields of the fractions of n-hexane-soluble, ethyl acetate-soluble and methanol-soluble from the P. densiflora sawdust were obtained 1.36%, 2.21% and 4.03% using organic solvents, respectively (Table 1). The antifungal activity of the n-hexane extracts against L. edodes at concentrations 125, 250, 500, 750 and 1000 ppm were 36.5%, 41.2%, 43.1%, 45.4% and 47.6%, respectively (Table 2). The percent of antifungal activity against L. edodes was the greatest for the n-hexane extracts, ranging from 36.5% to 47.6% at a concentrations of 125 ppm to 1000ppm, with the values for all concentrations significantly different from one another. In this experiment, the antifungal activity by the n-hexane extracts was the highest. This result supposed that the n-hexane extracts have not only many more inhibitory compounds against L. edodes than the ethyl acetate and methanol extracts but also the inhibitory compound may be more easily dissolved by n-hexane than by ethyl acetate or methanol.

The yields of the n-hexane-soluble, ethyl acetate-soluble, methanol-soluble and methanol-insoluble fractions from the powdered water extracts were 8.2%, 10.6%, 32.0% and 49.2%, respectively (Table 1). The antifungal activities of water extracts, n-hexane-soluble fraction, ethyl acetate-soluble fraction, methanol-soluble fraction and methanol-insoluble fraction against L. edodes were 23.5%, 26.2%, 41.5%, 25.4% and 2.5%, respectively, at 1000ppm (Figure3). The ethyl acetate-soluble fraction showed much higher antifungal activity than the other fractions. The antifungal activity of the ethyl acetate-soluble fraction against L. edodes at 1000 ppm showed a statistically significant difference in the fractions of n-hexane-soluble, ethyl acetate-soluble, methanol-soluble and methanol-insolub

맛버섯균의 균사 배양을 위한 최적배지는 버섯완전배지 (MCM), 최적균사 생장온도는 25 C, 최적pH는 5.0이었다. 균사생장이 가장 양호한 탄소원은 GLUCOSE, MANNOSE, 질소원은 YEAST EXTRACT, PEPTONE 및 ASPARAGINE, 등 이었다. 톱밥 병재배시(850㎖) 30일 배양후 숙성일수에 따른 초발이소요일, 자실체 생육일수 및 수량을 검토한 결과 숙성하지 않은 배양 30일 처리시 초발이소요일 15∼20일 , 병당 수량 79g인 반면 숙성일수가 가장 긴 배양 70일 처리시 초발이 소요일 6∼9일, 병당 수량 98g으로 숙성 일수가 길수록 초발이소요일이 빠르고, 수량이 증가하는 경향이었다. PP봉지 재배시 배 850g 배지에서 초발이소요일 17∼21일, 갓의 수 39개, 수량 157g으로 배지 회수율이 가장 양호하였다.

꽃송이버섯은 베타글루칸 함량이 높아 버섯재배자들 사이에서 재배를 희망하는 농가들이 부쩍 늘어나고 있다. 하지만 이에 대한 실용적인 재배법이 확립되지 않아 까다로운 버섯으로 인식하여 재배를 기피하고 있어 본 실험에서는 침엽수 톱밥과 소맥분, 물엿을 이용한 간편하고 경제성 있는 배지조성법을 연구하였다. 톱밥 접종원과 액체 접종원을 사용한 경우의 균사 생장에는 뚜렷한 차이가 없었으며, 4℃에서 1일 동안 저온충격을 주는 방식이 원기 형성을 위하여 가장 좋은 방법으로 생각되었다. 또한 자실체 발생용 최적배지는‘미송+소맥분+옥수수+면실박+10% 물엿 수용액’으로 41%의 회수율을 보였으나,‘ 낙엽송+소맥분+옥수수+면실박+10% 물엿 수용액’에서도 회수율이 37%로 자실체 발생량이 많았다. 또한 회수율과 버섯배지 중량 감소율과의 관계를 보면 회수율이 높을수록 중량감소율이 높아 서로 비례한 것으로 조사되었다.

여름철 팽이버섯 병재배시 톱밥배지에서 발생하는 균사생장 억제증상의 원인을 구명하고 그 경감대책을 확립하기 위하여 톱밥배지 배합시 물온도를 24℃(상온수)와 6℃(저온수)로 하고, 배지제조후 살균전 경과시간을 0, 3, 6, 9시간으로 달리하여 팽이버섯의 재배시험을 수행하였다. 배지제조 후 살균전 시간이 경과함에 따라 배지온도가 상승하고, pH는 6.5에서 5.2∼5.6으로 낮아지는 경향이었다. 이때 톱밥배지 내에서 세균밀도가 증가하였으며, 물온도 24℃에서는 6℃보다 6시간 이후의 세균밀도가 1.9∼4.1배 높았다. 톱밥배지에서 분리한 세균과 대치배양시 팽이버섯균의 생장이 억제되었다. 또한 배지제조후 살균전 시간이 경과함에 따라 배지내의 전질소 함량이 감소하였으며 유리당의 함량도 종류에 따라서 증가 또는 감소하였다. 배지배합시 24℃물 처리구는 6℃물 처리구에 비하여 팽이버섯 병재배사에서 균배양기간 및 버섯발생기간이 각각 1∼2일 지연되는 경향이었으며, 자실체 수량도 저온수 처리구에서 안정적이었다. 그리고 9시간 경과한 배지에서는 균사생장이 정지되었다. 배지제조후 경과시간에 따라 팽이버섯 발생기간은 1∼3일이 지연이 되었으며, 자실체수량은 6시간이 경과된 배지에서 7∼12% 감소하는 경향이었다. 본 시험은 여름철 고온기의 팽이버섯 재배시 배지제조에 6℃이하의 저온수를 사용하여 배지내 미생물 증식을 억제시키고, 입병작업을 마치는 즉시 고압살균 함으로써 배지의 성분을 안정화시켜 버섯의 안정생산에 기여할 것으로 기대된다.

After having inoculated the mycelium of L. edodes on the block medium, the P. densiflora sawdust of the B and C groups were the slowest of their respective groups on day 169.4 and day 169.9 harvesting times. The first harvest of the fruiting body from the Q. variabilis sawdust of the control medium was obtained on day 162.9

여름 사료작물 피에 대해 톱밥 발효돈분의 비료효과를 구명하기 위하여 1998년 5월부터 10월까지 제주대학교 생명자원과학대학 사료작물 포장에서 시험이 수행되었다. 실험은 난괴법 6처리 3반복으로써 처리 1: 화학비료 정량, 처리 2: 화학비료 정량의 +톱밥 발효돈분 정량의 1/2 (N 100kg/ha), 처리 3: 톱밥 발효돈분 정량의 1/2(N 100kg/ha), 처리 4: 톱밥 발효돈분 정량(N 200kg/ha), 처리 5: 톱밥 발효돈분 정량의 2